Experimental gerontology最新文献

{"title":"Mild cognitive impairment among older adults in outpatient clinics: Awareness and knowledge needs survey","authors":"Hong Wang ,&nbsp;Jiao Li ,&nbsp;Xia-Fen Bai ,&nbsp;Fan Tian ,&nbsp;Ao-Feng Xu ,&nbsp;Lei Huang ,&nbsp;Meng Wang ,&nbsp;Yuan Yang","doi":"10.1016/j.exger.2025.112834","DOIUrl":"10.1016/j.exger.2025.112834","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to assess the current awareness and knowledge requirements related to mild cognitive impairment (MCI) among older adults in hospital outpatient departments, serving as a reference for designing cognitive health intervention strategies tailored to older adults.</div></div><div><h3>Methods</h3><div>A custom-designed questionnaire was administered to survey 132 older adults attending outpatient clinics.</div></div><div><h3>Results</h3><div>The MCI symptom awareness score among older adults in outpatient clinics was 7.45 ± 3.58 points. The average correct identification rate for MCI cases was 55.1 %, while the misclassification rate for non-MCI cases reached 38.8 %. Among participants, 38.6 % would be unwilling to disclose an MCI/dementia diagnosis if they or a family member were affected, and 25.0 % believed that older adults with MCI/dementia would face social contempt. The most preferred source for cognitive impairment prevention methods was hospital consultations (92.4 %), followed by television, radio, newspapers, the internet, and other media channels (57.6 %), and outpatient clinic or community health lectures (56.8 %). Participants sought information primarily on MCI symptoms (64.4 %), etiology, and risk factors (60.6 %), among other topics.</div></div><div><h3>Conclusion</h3><div>Approximately 50 % of older adults in outpatient clinics accurately identified MCI symptoms; however, misconceptions and misjudgments remained. Some older adults were reluctant to disclose their condition. Hospital consultations were the preferred source for obtaining cognitive impairment prevention methods. Enhancing public education on cognitive impairment is essential to help older adults accurately recognize MCI symptoms, seek early screening, and adopt preventive measures, thereby mitigating disease progression and minimizing its impact on their quality of life.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"209 ","pages":"Article 112834"},"PeriodicalIF":3.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cognitive impairment mediates the association between sarcopenic obesity and daily living ability in the frail older population","authors":"Hang Li ,&nbsp;Haifei Shen ,&nbsp;Lei Yu ,&nbsp;Yao Wen ,&nbsp;Dan Ren ,&nbsp;Cong Ding ,&nbsp;Xiaobo Wang ,&nbsp;Wanjun Xie ,&nbsp;Yongwang Miao ,&nbsp;Wenxu Zheng","doi":"10.1016/j.exger.2025.112833","DOIUrl":"10.1016/j.exger.2025.112833","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the relationships between sarcopenic obesity, cognitive function, and daily living ability in a hospitalized frail older population.</div></div><div><h3>Method</h3><div>We enrolled 206 older adults hospitalized frail or prefrail patients aged ≥60 years for body composition analysis, motor and cognitive function assessment, and daily living ability assessment. Cognitive impairment and daily living ability were measured using the Mini-Mental State Examination (MMSE) and Barthel Index (BI) score, respectively. Sarcopenia was diagnosed according to the Asian Working Group for Sarcopenia 2019 consensus. Obesity was defined by body mass index (BMI) (≥ 28 kg/m²) or body fat mass percentage (≥ 60th percentile in each sex group). Participants were divided into normal (N), simple obesity (O), sarcopenia(S), and sarcopenic obesity (SO) groups. We used correlation analysis, multiple logistic regression analysis, and mediation analysis to assess the associations between sarcopenic obesity, cognitive function and daily living ability.</div></div><div><h3>Results</h3><div>The SO group had the highest prevalence of cognitive impairment and life activity dependency. Correlation analysis showed that the MMSE score was positively correlated with the BI score (<em>r</em> = 0.61, <em>P</em> &lt; 0.01). The MMSE score was positively correlated with the physical performance and negatively correlated with obesity indicators (<em>P</em> &lt; 0.01). According to our mediation analysis, SO and BI score had significant direct effects (coefficients = 10.13, 95 % CI: 5.76–14.50) and total effects (coefficients = 17.44, 95 % CI: 12.65–22.23). When cognitive impairment (MMSE&lt;27) was added to the model, cognitive impairment still had an indirect (i.e., mediating) effect on the relationship between SO and BI score (coefficients = 7.31, 95 % CI: 4.05–11.11), and 41.92 % of the total effect was attributable to mediation by cognitive impairment.</div></div><div><h3>Conclusion</h3><div>Sarcopenic obesity is closely related to cognitive impairment and decreased daily living ability. Sarcopenic obesity can affect daily living ability through the mediating effect of cognitive impairment. Therefore, more attention should be paid to the evaluation and intervention of cognitive function in order to improve the daily living ability of the older adults population with sarcopenic obesity.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"209 ","pages":"Article 112833"},"PeriodicalIF":3.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chronic low dose of Δ9-tetrahydrocannabinol (3 mg / kg / 21 d) reorganizes the disturbed wound healing process and accelerates wound closure in old female mice","authors":"Melissa Plum,&nbsp;Justus P. Beier,&nbsp;Tim Ruhl","doi":"10.1016/j.exger.2025.112832","DOIUrl":"10.1016/j.exger.2025.112832","url":null,"abstract":"<div><div>Wound healing in old mice is characterized by disturbed tissue homeostasis, manifested by delayed immune cell infiltration and reduced growth factor secretion, leading to a delayed onset and prolonged duration of the inflammatory phase. The endocannabinoid system (ECS) is an important regulator of tissue homeostasis and cell migration and is also considered to be subject to aging processes, which may contribute to observable aging phenomena. Therefore, stimulating the aged ECS could represent a therapeutic option to support tissue regeneration in aging.</div><div>Female old mice received a low-dose of medical THC daily for 3 weeks, before four excisional full skin wounds were created. At day 1, 3 and 7 post-surgery, the wound closure rate was analyzed and wound samples were examined immunohistochemically for the numbers of granulocytes, M1-macrophages and mesenchymal stem cells (MSCs). The concentrations of inflammatory cytokines and regenerative growth factors were determined by ELISA.</div><div>Administration of THC improved the wound healing rate of old mice between day 1 and 7, which was associated with an altered timing and quantity of infiltrating immune cells and decreased levels of inflammatory cytokines in wound tissue on days 1 and 3 post-injury. THC treatment significantly increased MSC infiltration but had no effect on the growth factor release.</div><div>The present study confirmed the anti-inflammatory activity of THC <em>in vivo</em>. The THC-treatment improved wound healing in old mice by coordinating the temporal sequence of immune cell infiltration and cytokine release. Thus, restoration of ECS signaling could be an effective strategy to support age-related skin regeneration.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"208 ","pages":"Article 112832"},"PeriodicalIF":3.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerobic exercise preconditioning suppresses subcutaneous tumorigenesis via PI3K/AKT-driven metabolic epigenetic reprogramming and immune microenvironment remodeling","authors":"Xue Zhang ,&nbsp;Yixue Xia ,&nbsp;Lingbing Zheng ,&nbsp;Lu Cao ,&nbsp;Zhengtang Qi ,&nbsp;Shuzhe Ding","doi":"10.1016/j.exger.2025.112824","DOIUrl":"10.1016/j.exger.2025.112824","url":null,"abstract":"<div><div>It has been widely recognized that aerobic exercise inhibits tumors, and aerobic glycolysis is closely related to tumor development. Whether exercise preconditioning inhibits subcutaneous tumor growth remains unclear. This study aimed to investigate the effect of aerobic exercise preconditioning on subcutaneous tumors in tumor-bearing mice and determine its mechanism. We first subjected the mice to aerobic exercise for 12 weeks, followed by the subcutaneous transplantation of Hepa1–6 cells under the right armpit. Compared with the control group, aerobic preconditioning significantly inhibited Hepa1–6 tumor growth and reduced the expression of PKM2, GLUT1, LDHA, and HK2 in tumor tissues. RNA-Seq showed that differentiated gene expression in the aerobic exercise group was enriched in the PI3K/AKT signaling pathway. Western blot detection showed that P-PI3K and P-AKT proteins were significantly downregulated in the tumor tissue of the exercise group. Meanwhile, RNA-Seq showed that exercise downregulated the expression of genes related to glucose metabolism. The TISCH2 database single cell population gene cluster map (macrophage population) suggested that these genes were closely related to immune cell infiltration. Immunofluorescence assay also showed that exercise significantly reduced the inflammatory infiltration of M1 macrophages. Moreover, in mice with DEN-induced HCC, the anticancer effects of aerobic exercise were related to liver DNA methylation, and the differential methylation sites were enriched in the PI3K/Akt pathway. Therefore, the antitumor effect of exercise intervention is directly related to the epigenetic reprogramming of tumor metabolism, suggesting that the PI3K/AKT pathway is the central component in the exercise regulation of tumor metabolism.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"209 ","pages":"Article 112824"},"PeriodicalIF":3.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144628325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults","authors":"Michael J. Toth ,&nbsp;Patrick D. Savage ,&nbsp;Deena B. Snoke ,&nbsp;Emma R. Bellefleur ,&nbsp;Michael DeSarno ,&nbsp;Timothy W. Tourville ,&nbsp;Michael Blankstein ,&nbsp;Alexander R. Keeble ,&nbsp;Sara Gonzalez-Velez ,&nbsp;Christopher S. Fry ,&nbsp;Jennifer Stevens-Lapsley ,&nbsp;Nathaniel J. Nelms","doi":"10.1016/j.exger.2025.112831","DOIUrl":"10.1016/j.exger.2025.112831","url":null,"abstract":"<div><div>Knee osteoarthritis (OA) is the leading cause of physical disability in older adults. Total knee arthroplasty (TKA) is a common treatment for advanced stage knee OA that alleviates knee pain, but it is associated with precipitous reductions in physical function early after surgery that can take months or years to recover. Sustaining neuromuscular activation after surgery with neuromuscular electrical stimulation (NMES) can improve recovery of physical function, but the mechanisms underlying its benefits are unclear. To examine the unique effects of NMES on skeletal muscle, we randomized older adult patients (70 % female) to early NMES (<em>n</em> = 11) or no intervention (<em>n</em> = 12) for 5 weeks after surgery. We measured skeletal muscle (vastus lateralis) fiber size, contractility, mitochondrial content, and mRNA abundance pre-surgery and 5 weeks post-surgery. NMES diminished TKA-induced muscle fiber atrophy in fast-twitch, myosin heavy chain (MHC) IIA fibers and improved or preserved single muscle fiber contractility in MHC I and MHC IIA fibers, respectively. In MHC IIA fibers, the beneficial effects of NMES to sustain fiber force production were explained at the molecular level by preservation of strongly bound, myosin-actin crossbridges. Additionally, TKA-induced increases in markers of denervation (<em>CHRNA1</em> and <em>MYOG</em>) in controls were prevented by NMES. Our results identify beneficial effects of sustaining neuromuscular activation early, post-TKA with NMES on skeletal muscle fiber size and function and potential molecular mechanisms underlying these effects.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"209 ","pages":"Article 112831"},"PeriodicalIF":3.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144628326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of chondrocyte senescence in osteoarthritis pathogenesis and therapeutic implications","authors":"Kai Huang ,&nbsp;Haili Cai","doi":"10.1016/j.exger.2025.112828","DOIUrl":"10.1016/j.exger.2025.112828","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a common and debilitating joint disorder, with its pathogenesis significantly influenced by factors such as aging and obesity. A critical aspect of OA development is the senescence of chondrocytes, which is characterized by irreversible cell cycle arrest and the secretion of pro-inflammatory molecules, collectively known as the senescence-associated secretory phenotype (SASP). Senescent chondrocytes compromise the maintenance of the extracellular matrix (ECM) and accelerate cartilage degradation, thereby exacerbating the progression of OA. Contributing factors to chondrocyte senescence include oxidative stress, mechanical overload, and ECM stiffness, while cellular dysfunctions such as mitochondrial impairment and defective autophagy further promote cartilage deterioration. Emerging therapeutic strategies aim to target senescent chondrocytes through the use of senolytic and senomorphic agents, microRNA-based therapies, immunotherapies, and stem cell-based interventions. Although these treatments have demonstrated potential in preclinical studies, additional research is required to enhance their efficacy in the clinical management of OA.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"208 ","pages":"Article 112828"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the effect of ambient temperature, sound type, and noise level on the heart rate of the elderly","authors":"Mohammad Tohidi ,&nbsp;Karen Fatahi ,&nbsp;Maryam Beigi","doi":"10.1016/j.exger.2025.112829","DOIUrl":"10.1016/j.exger.2025.112829","url":null,"abstract":"<div><h3>Background</h3><div>Given the increasing elderly population in the future, attention to the health of older adults is important. Environmental factors, including ambient temperature, sound type, and noise level, greatly affect the health of older adults. This study looks at how these elements influence the heart rate of elderly individuals.</div></div><div><h3>Method</h3><div>This study involves laboratory research with 216 elderly male participants from Ilam province in Iran over four months, from May to August 2024. Participants were exposed to different ambient temperature settings (20 °C, 25 °C, and 30 °C) and four noise levels (55, 65, 75, and 85 dB(A)) using sound types like music, traffic, and crowded noise. They were randomly divided into three equal groups of 72 by ambient temperature and then into subgroups of 24 based on the three sound type; finally, these subgroups were tested under four different noise levels, with each group consisting of 6 participants. The data from participants were analyzed using a three-way ANOVA model.</div></div><div><h3>Results</h3><div>The findings showed that ambient temperature, sound type, and noise level greatly affected older adults' heart rate, with strong statistical significance. There was a notable interaction between ambient temperature and noise level. However, other interactions did not show significant relationships with heart rate.</div></div><div><h3>Conclusion</h3><div>The study highlights important impacts on the health of elderly in noisy urban areas. It shows that long exposure to high noise and heat increases heart rate. This research emphasizes noise control and heat management to protect older adults and informs public health policies and urban planning.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"208 ","pages":"Article 112829"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood-detected mitochondrial biomarker NSUN4: a potential indicator of ovarian aging","authors":"Jianheng Hao ,&nbsp;Liying Liu ,&nbsp;Boya Chang ,&nbsp;Yuemeng Zhao ,&nbsp;Yuanyuan Lai ,&nbsp;Chunhui Tian ,&nbsp;Huichao Xu ,&nbsp;Haijun Wang ,&nbsp;Laixi Ji ,&nbsp;Jie Yang","doi":"10.1016/j.exger.2025.112825","DOIUrl":"10.1016/j.exger.2025.112825","url":null,"abstract":"<div><h3>Background</h3><div>Mitochondrial dysfunction is a key hallmark of aging, and blood-based biomarkers related to mitochondrial genes provide an effective means to assess ovarian aging progression. In this study, we aimed to explore the role of mitochondrial dysfunction-related genetic variations in determining the natural age at menopause (ANM) by applying both Mendelian randomization (MR) and summary data-based Mendelian randomization (SMR) approaches, complemented by experimental validation in animal models.</div></div><div><h3>Methods</h3><div>Summary statistics on ANM, gene expression, DNA methylation, and protein abundance quantitative trait loci (eQTL, mQTL, pQTL) were obtained from public databases. Genetic variations associated with mitochondrial dysfunction were selected as instrumental variables, and SMR analysis was performed to investigate causal relationships with ANM. MR methods were also used to evaluate the causal effect of mitochondrial DNA copy number (mtDNA-CN) on ANM, with preliminary validation through animal experiments.</div></div><div><h3>Results</h3><div>SMR and meta-analysis results identified NSUN4 as a critical regulator of ANM at both the gene expression and DNA methylation levels. A preliminary causal relationship between reduced mtDNA-CN and increased ANM risk was found, though further validation with larger datasets is needed. Animal experiments indicated that NSUN4 levels in blood reflect ovarian function decline and may correlate with its expression in ovarian tissue.</div></div><div><h3>Conclusions</h3><div>The findings suggest that NUSN4 levels detected in the blood could serve as a potential biomarker for ovarian aging. This provides new insights into the role of mitochondrial dysfunction in reproductive age-related traits and may inform future targeted interventions to slow ovarian aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"208 ","pages":"Article 112825"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Assessment of muscle function deterioration in aging populations: Insights from the load-velocity relationship during the loaded sit-to-stand test” [Exp. Gerontol. 206 (2025) 112767]","authors":"Daniel Jiménez-Lupión ,&nbsp;Ignacio Chirosa Ríos ,&nbsp;Rodrigo Lizama-Pérez ,&nbsp;Luis Chirosa-Ríos ,&nbsp;Daniel Jerez-Mayorga","doi":"10.1016/j.exger.2025.112827","DOIUrl":"10.1016/j.exger.2025.112827","url":null,"abstract":"","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"208 ","pages":"Article 112827"},"PeriodicalIF":3.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture modulates ovarian senescence through metabolic reprogramming: A multi-omics investigation in chemotherapy - induced POF model","authors":"Wei Dandan ,&nbsp;Li Shanshan ,&nbsp;Zhu Ranpei ,&nbsp;He Zongqing ,&nbsp;Qiu Fan ,&nbsp;Qu Wenwen ,&nbsp;Liu Xiaopeng ,&nbsp;Lou Yujie ,&nbsp;Wei Yurun ,&nbsp;Wang Qingbo","doi":"10.1016/j.exger.2025.112815","DOIUrl":"10.1016/j.exger.2025.112815","url":null,"abstract":"<div><div>Premature ovarian failure (POF) is a common female endocrine disorder in women, and there is currently no effective pharmacological treatment available. Acupuncture, an important component of traditional Chinese medicine, has demonstrated clinical efficacy in improving ovarian function in patients with POF; however, its underlying mechanisms remain unclear. This study systematically investigated the therapeutic mechanisms of acupuncture in cyclophosphamide (CTX)-induced POF through combined analysis of proteomic and metabolomic data. In a rat model of chemotherapy-induced ovarian dysfunction, 28-day acupuncture intervention significantly delayed the pathological alterations in ovarian morphology and slowed the decline of endocrine function, as evidenced by normalized serum FSH, LH, and AMH levels. Metabolomic profiling identified 1683 differential metabolites across positive/negative ion modes, with taurine-hypotaurine metabolism (KEGG map00430) emerging as a key antioxidant axis, correlating with enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. Proteomic analysis revealed acupuncture-mediated reversal of 115 ovarian proteins, particularly PPAR signaling components (FABP5, ADIPOQ and PLIN1), which orchestrated lipid metabolic reprogramming. Combined analysis of proteomic and metabolomic data demonstrated strong Spearman correlations between metabolic regulators (taurine, <span>d</span>-aspartate) and ovarian reserve markers, while PPAR-associated proteins exhibited network connectivity with oxidative stress mediators. These findings establish that acupuncture delays POF through synchronized modulation of metabolic flexibility and PPARγ-driven antioxidant defense, providing a novel systems biology framework for understanding traditional medicine interventions in reproductive aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"209 ","pages":"Article 112815"},"PeriodicalIF":3.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}