Experimental gerontology最新文献

{"title":"Young bone marrow transplantation delays bone aging in old mice","authors":"Lina Abu-Nada ,&nbsp;Younan Liu ,&nbsp;Faez Saleh Al-Hamed ,&nbsp;Bouchra Ouliass ,&nbsp;Magali Millecamps ,&nbsp;Simon D. Tran ,&nbsp;Guylaine Ferland ,&nbsp;Vahab D. Soleimani ,&nbsp;Faleh Tamimi Marino ,&nbsp;Monzur Murshed","doi":"10.1016/j.exger.2025.112704","DOIUrl":"10.1016/j.exger.2025.112704","url":null,"abstract":"<div><div>Recent discoveries have shown that systemic manipulations, such as parabiosis, blood exchange, and young plasma transfer, can counteract many hallmarks of aging. This rejuvenation effect has been attributed to circulatory factors produced by cells from both hematopoietic and non-hematopoietic lineages. However, the specific involvement of bone marrow (BM) or hematopoietic cells in producing such factors and their effects on aging is still unclear. We developed a model of aged mice with transplanted young or old BM cells and assessed the impact on the aging process, specifically on energy metabolism and bone remodeling parameters. The donor BM cell engraftment in the aged mice was confirmed by flow cytometry using a transplanted cell-specific marker (green fluorescent protein). Energy metabolism was assessed using Oxymax indirect calorimetry system after 3 months of transplantation. Tibiae and L3-L4 vertebrae were analyzed using micro-CT, a three-point bending test and bone histomorphometry. Moreover, bone marrow proteome was assessed using proteomics, and blood serum/plasma was collected and analyzed using the Luminex assay. Our results showed that while the effect on energy metabolism was insignificant, rejuvenating the BM through young bone marrow transplantation reversed age-associated low bone mass traits in old mice. Specifically, young bone marrow transplantation improved bone trabecular microarchitecture both in tibiae and vertebrae of old mice and increased the number of osteoblasts and osteoclasts compared to old bone marrow transplantation. In conclusion, young bone marrow cells may represent a future therapeutic strategy for age-related diseases such as osteoporosis. The findings of this study provide important insights into our understanding of aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112704"},"PeriodicalIF":3.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhythms and shifts of chemokines and cytokines interplay in a decade lifespan: The longitudinal community-based Bambuí health and aging study","authors":"Joaquim Pedro Brito-de-Sousa ,&nbsp;Maria Luiza Lima-Silva ,&nbsp;Ismael Artur Costa-Rocha ,&nbsp;Ana Carolina Campi-Azevedo ,&nbsp;Juliana Vaz de Melo Mambrini ,&nbsp;Ana Maria Caetano Faria ,&nbsp;Maria Fernanda Lima-Costa ,&nbsp;Sérgio Viana Peixoto ,&nbsp;Andréa Teixeira-Carvalho ,&nbsp;Karen Cecília Lima Torres ,&nbsp;Olindo Assis Martins-Filho","doi":"10.1016/j.exger.2025.112700","DOIUrl":"10.1016/j.exger.2025.112700","url":null,"abstract":"<div><div>Aging is associated with several physiological changes, including a remarkable remodeling of the immune system. Herein, the rhythms and shifts in serum immune mediators were characterized in a decade lifespan as a longitudinal community-based prospective investigation from Bambuí Health and Aging Study. The study population included paired samples from 713 subjects survivors from the original BHAS cohort and at 10-years Follow-up, categorized into 5-years age range intervals (60-64^Yrs towards 90 + ^Yrs). Quantification of soluble mediators were carried out by Cytometric Bead Array. The results demonstrated a rhythmic increase in serum immune mediators, especially CXCL9, CXCL10, IL-1β, IL-6 and TNF following the aging process, particularly at age intervals 70-74^Yrs and 85-89^Yrs. More prominent fold change magnitudes were observed for TNF (27.64×), CXCL9 (2.40×), IL-1β (2.20×), IL-6 (1.47×), and CXCL10 (1.26×). On the other hand, analysis of integrative networks showed a waning in the correlation numbers between immune mediators in a decade lifespan and a shift of connectivity from chemokines at Enrollment towards cytokines at 10-years Follow-up. Cross-correlation approaches revealed that CXCL9, CXCL10, IL-1β, IL-6, and IL-10 were placed in the innermost position, underscoring the higher contribution of these mediators along aging. Overall, these findings re-emphasize the impact of aging in the dynamic profile of serum immune mediators, highlighting the shift of selective mediators and their rhythmic signatures across chronological aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112700"},"PeriodicalIF":3.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The causal relationship of inflammation-related factors with osteoporosis: A Mendelian Randomization Analysis","authors":"Xinyue Yang ,&nbsp;Rui Xiao ,&nbsp;Beizhong Liu ,&nbsp;Bo Xie ,&nbsp;Zhao Yang","doi":"10.1016/j.exger.2025.112715","DOIUrl":"10.1016/j.exger.2025.112715","url":null,"abstract":"<div><h3>Background</h3><div>We used Mendelian randomization (MR) approach to examine whether genetically determined inflammation-related risk factors play a role in the onset of osteoporosis (OP) in the European population.</div></div><div><h3>Methods</h3><div>Genome-wide association studies (GWASs) summary statistics of estimated bone mineral density (eBMD) obtained from the public database GEnetic Factors for OSteoporosis Consortium (GEFOS) including 142,487 European people. For exposures, we utilized GWAS data of 9 risk factors including diseases chronic kidney disease (CKD) (41,395 cases and 439,303 controls), type 2 diabetes (T2D) (88,427 cases and 566,778 controls), Alzheimer's disease (AD) (71,880 cases, 383,378 controls) and major depression disorder (MDD) (9240 cases and 9519 controls) and lifestyle behaviors are from different consortiums. Inverse variance weighted (IVW) analysis was principal method in this study and random effect model was applied; MR-Egger method and weighted median method were also performed for reliable results. Cochran's Q test and MR-Egger regression were used to detect heterogeneity and pleiotropy and leave-one-out analysis was performed to find out whether there are influential SNPs.</div></div><div><h3>Results</h3><div>We found that T2D (<em>IVW: β =</em> <em>0.05, P</em> <em>=</em> <em>0.0014</em>), FI (<em>IVW: β = −0.22, P</em> <em>&lt;</em> <em>0.001</em>), CKD (<em>IVW: β =</em> <em>0.02, P</em> <em>=</em> <em>0.009</em>), ALZ (<em>IVW: β =</em> <em>0.06, P</em> <em>=</em> <em>0.005</em>), Coffee consumption (<em>IVW: β = 0.11, P</em> <em>=</em> <em>0.003</em>) were causally associated with OP (<span><math><mi>P</mi><mo>&lt;</mo><mn>0.006</mn><mspace></mspace></math></span>after Bonferroni correction).</div></div><div><h3>Conclusions</h3><div>Our study revealed that T2D, FI, CKD, ALZ and coffee consumption are causally associated with OP. Future interventions targeting factors above could provide new clinical strategies for the personalized prevention and treatment of osteoporosis.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112715"},"PeriodicalIF":3.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Associations of daily steps and step intensity with peripheral arterial disease in Chinese community-dwelling older women” [Exp. Gerontol. 201 (2025) 112706]","authors":"Chenfei Li ,&nbsp;Litao Du ,&nbsp;Xiangli Xue ,&nbsp;Na Zhao ,&nbsp;Qiang He ,&nbsp;Si Chen ,&nbsp;Xianliang Zhang","doi":"10.1016/j.exger.2025.112717","DOIUrl":"10.1016/j.exger.2025.112717","url":null,"abstract":"","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112717"},"PeriodicalIF":3.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular senescence and glaucoma","authors":"Liang Guo ,&nbsp;Na Wang ,&nbsp;Jing Chen ,&nbsp;Rui Zhang ,&nbsp;Dan Li ,&nbsp;Lu Yang","doi":"10.1016/j.exger.2025.112718","DOIUrl":"10.1016/j.exger.2025.112718","url":null,"abstract":"<div><div>Cellular senescence, a characteristic feature of the aging process, is induced by diverse stressors. In recent years, glaucoma has emerged as a blinding ocular disease intricately linked to cellular senescence. The principal pathways implicated are oxidative stress, mitochondrial dysfunction, DNA damage, autophagy impairment, and the secretion of various senescence- associated secretory phenotype factors. Research on glaucoma-associated cellular senescence predominantly centers around the increased resistance of the aqueous humor outflow pathway, which is attributed to the senescence of the trabecular meshwork and Schlemm's canal. Additionally, it focuses on the mechanisms underlying retinal ganglion cell senescence in glaucoma and the corresponding intervention measures. Given that cell senescence represents an irreversible phase preceding cell death, an in-depth investigation into its mechanisms in the pathogenesis and progression of glaucoma, particularly by specifically blocking the signal transduction of cell senescence, holds the potential to decrease the outflow resistance of aqueous humor. This, in turn, could provide a novel avenue for safeguarding the optic nerve in glaucoma.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112718"},"PeriodicalIF":3.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of small needle knife in treating inflammatory factors, Th17 cell function, and magnetic resonance imaging evaluation in osteoporosis complicated by compression fractures in elderly patients","authors":"Jian Wang ,&nbsp;Hua Xiao ,&nbsp;Yuanqiang Liang","doi":"10.1016/j.exger.2025.112719","DOIUrl":"10.1016/j.exger.2025.112719","url":null,"abstract":"<div><h3>Objective</h3><div>The incidence of osteoporosis complicated by vertebral compression fractures (OVCF) has been increasing annually, and percutaneous vertebroplasty (PVP) is the main surgical approach for it. It was to evaluate the use of a small needle knife (SNF) in conjunction with PVP to assess changes in inflammatory response, Th17 cell function, and magnetic resonance imaging (MRI) parameters in OVCF patients.</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted using the clinical data of 138 patients with OVCF, who were categorized into PVP group (<em>n</em> = 65) and PVP + SNF group (<em>n</em> = 73). MRI was employed to evaluate changes in lumbar spine anatomy and paraspinal muscle parameters. The postoperative thoracic and intercostal pain and joint function recovery were assessed. Blood samples were collected to measure inflammatory factors, Th17 cell proportions, and related cytokine levels.</div></div><div><h3>Results</h3><div>PVP + SNF group showed decreased visual analogue scale (VAS) scores at 3 days, 7 days, and 1 month postoperatively versus PVP group (<em>P</em> &lt; 0.05). MRI examination indicated significant improvements in anterior vertebral height, posterior vertebra height, multifidus cross-sectional area, and erector spinae cross-sectional area, along with a decrease in steatosis proportion in the PVP + SNF group (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>SNF in combination with PVP reduced postoperative pain and improved lumbar spine function in OVCF patients. Furthermore, it can lower inflammatory responses by enhancing Th17 cell function. MRI examinations can serve as valuable tools for predicting and assessing postoperative outcomes in OVCF patients.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112719"},"PeriodicalIF":3.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of 15-PGDH by SW033291 ameliorates age-related heart failure in mice","authors":"Li Zhang ,&nbsp;Qiang Wang ,&nbsp;Wenjun Guan","doi":"10.1016/j.exger.2025.112710","DOIUrl":"10.1016/j.exger.2025.112710","url":null,"abstract":"<div><div>Chronic loss of cardiomyocyte integrity underlies human heart failure associated with aging that often involves progression of acute myocardial infarction and the maladaptive response of cardiomyopathy. SW033291, an inhibitor of 15-prostaglandin dehydrogenase (15-PGDH), has been shown to mitigate fibrosis of mice heart. Whether it has cardioprotective effect remain unknown. Young and aged C57BL/6 J mice were treated with either the vehicle or SW033291 for four weeks. The expression of the target gene was assessed by RT-qPCR, Western blotting, and ELISA. Cardiac function was measured by echocardiography. Our study demonstrated that SW033291 induced a notable upregulation of prostaglandin E2 while concurrently downregulated the expression of both 15-PGDH and troponin I in cardiac tissues, encompassing both young and aged mice. Notably, the administration of SW033291 resulted in a significant improvement in systolic and diastolic function among aged mice, although this effect was not observed in their younger counterparts. Subsequent investigations focusing on exploring the mechanisms, revealed that repetitive administration of SW033291 effectively mitigated age-induced oxidative stress and curtailed chronic inflammation within the cardiac tissues of aged mice. These pivotal findings establish a solid foundation for contemplating the prospective therapeutic application of SW033291 in addressing age-related heart failure.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112710"},"PeriodicalIF":3.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biology of sex differences in frailty and aging: Where are we?","authors":"Beatrice Arosio ,&nbsp;Emanuele Marzetti ,&nbsp;Anna Picca","doi":"10.1016/j.exger.2025.112711","DOIUrl":"10.1016/j.exger.2025.112711","url":null,"abstract":"","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112711"},"PeriodicalIF":3.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statin is associated with higher cortical thickness in early Alzheimer's disease","authors":"Yane Zheng ,&nbsp;Huiying Gu ,&nbsp;Yuming Kong ,&nbsp;Alzheimer's Disease Neuroimaging Initiative (ADNI)","doi":"10.1016/j.exger.2025.112698","DOIUrl":"10.1016/j.exger.2025.112698","url":null,"abstract":"<div><h3>Background</h3><div>The brain is the most cholesterol-rich organ, essential for myelination and neuronal function. Statins, widely used to lower cholesterol, cross the blood-brain barrier and may impact brain cholesterol synthesis. Despite their widespread use, the effects of statins on cortical regions relevant to Alzheimer's disease (AD) are not well understood. This study aimed to compare cortical thickness between statin-exposed and statin-unexposed older adults and evaluate the potential neuroprotective effects of statins.</div></div><div><h3>Methods</h3><div>Data were obtained from the Alzheimer's Disease Neuroimaging Initiative (ADNI). The sample included 193 healthy controls (HC), 485 individuals with mild cognitive impairment (MCI), and 169 individuals with Alzheimer's disease (AD). Participants were categorized as statin users if they had used statins for at least two years. MRI data were processed using FreeSurfer software to estimate cortical thickness in 64 regions of interest. ANCOVA models assessed the association between statin use and cortical thickness at baseline, and linear mixed models evaluated longitudinal changes.</div></div><div><h3>Results</h3><div>Statin use was associated with increased cortical thickness in multiple brain regions across HC, MCI, and AD participants. In HC, statin users had greater thickness in the right lateral occipital, left middle temporal, and left parahippocampal regions. MCI participants exhibited additional increases in the right cuneus, right posterior cingulate, and left superior temporal cortex. In AD, statin users had higher thickness in the right cuneus and right superior parietal lobule. Longitudinal analysis revealed no statin-related differences in cortical thickness changes among HC and AD groups, but in MCI, statins slowed cortical thinning in the left medial orbitofrontal cortex.</div></div><div><h3>Conclusion</h3><div>Statin use is associated with greater cortical thickness in older adults, particularly in those with MCI. These findings suggest that statins may have neuroprotective effects, potentially mitigating neurodegenerative changes in early cognitive decline. Further research with larger cohorts and longer follow-up periods is needed to confirm these findings and understand the mechanisms involved.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112698"},"PeriodicalIF":3.9,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between urinary trace elements levels and depressive symptoms among the older population","authors":"Ying Zhang ,&nbsp;Junjiao Ping ,&nbsp;Dong Cui ,&nbsp;Zhenkun Tan ,&nbsp;Jiali Luo ,&nbsp;Chuijia Kong ,&nbsp;Na Xiao ,&nbsp;Haiyan Lv ,&nbsp;Xinxia Liu","doi":"10.1016/j.exger.2025.112709","DOIUrl":"10.1016/j.exger.2025.112709","url":null,"abstract":"<div><h3>Background</h3><div>Late-life depression is a prevalent public health issue among the elderly. Imbalances in trace elements are increasingly recognized as associated with depression; however, the majority of current research has concentrated on examining the link between blood-based trace elements levels and depressive symptoms. Our objective was to determine if a similar correlation is observed between urinary trace elements levels and depressive symptoms.</div></div><div><h3>Methods</h3><div>We employed stratified, multi-stage random sampling to recruit 400 participants, aged 60 years or older, from a community-based population in a city located in southern China. The Patient Health Questionnaire-9 Items (PHQ-9) was utilized to evaluate depressive symptoms. The concentration of trace elements in urine was detected by inductively coupled plasma mass spectrometry. Multiple logistic regression analysis was conducted to assess the association between urinary trace elements levels and depressive symptoms, as well as the interactions between these levels and potential covariates. The Restricted Cubic Spline (RCS) model with four knots to further explore the association between urinary trace elements and depressive symptoms risk after adjusting for the confounders.</div></div><div><h3>Results</h3><div>A total of 391 participants were investigated, including 50 (12.6 %) in depressive symptom group and 341 (87.4 %) in non-depressive symptom group. Urinary copper levels were positively correlated with depressive symptoms. Compared with the lowest tertile of urinary copper, the multivariate adjusted odds ratios of depressive symptom were 2.58 (1.18–5.64) in tertile 3. Furthermore, we found the interactions between urinary copper and gender were <em>p</em> &lt; 0.05. The multivariate correction OR for T3 versus T1 in males was 21.10 (1.79–248.13) (<em>P</em>_{for trend} = 0.002). RCS analysis revealed a positive association between copper levels and depressive symptoms (<em>P</em>-overall association = 0.025, and <em>P</em>-nonlinear = 0.161). No significant difference was observed in the risk of developing depressive symptoms among individuals with urinary copper concentrations below 8.22 μg/g creatinine. However, the risk of depressive symptoms increases progressively as the urinary copper concentration exceeds this threshold.</div></div><div><h3>Conclusion</h3><div>Urinary copper levels are correlated with the development of depressive symptoms, and copper exposure in men is more sensitive to depressive symptoms. Urinary copper, as a non-invasive test, is a promising indicator of depression symptoms in environmental exposure.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112709"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}