Canadian journal of microbiology最新文献_第6页

Three-dimensional buckling model reveals the evolution of energy-driven biofilm wrinkle morphologies. 三维屈曲模型揭示了能量驱动生物膜皱折形态的演化过程。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0196

Jin Wu, Jin Li, Jiankun Wang, Xiaoling Wang

引用次数: 0

Lavandula angustifolia oil induces oxidative stress, stiffening of membranes, and cell wall in Cryptococcus spp. 薰衣草精油诱导隐球菌氧化应激、膜硬化和细胞壁硬化。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-12-02 DOI: 10.1139/cjm-2024-0084

Yohana Porto Calegari-Alves, Rafael Lopes da Rosa, Renata Pereira Costa, Camila Innocente-Alves, Aline Martins Faustino, John R Yates, Walter Orlando Beys-da-Silva, Lucélia Santi

{"title":"Lavandula angustifolia oil induces oxidative stress, stiffening of membranes, and cell wall in Cryptococcus spp.","authors":"Yohana Porto Calegari-Alves, Rafael Lopes da Rosa, Renata Pereira Costa, Camila Innocente-Alves, Aline Martins Faustino, John R Yates, Walter Orlando Beys-da-Silva, Lucélia Santi","doi":"10.1139/cjm-2024-0084","DOIUrl":"10.1139/cjm-2024-0084","url":null,"abstract":"The Cryptococcus neoformans and Cryptococcus gattii species complexes are the etiological agents of cryptococcosis, a disease responsible for 181 000 deaths annually worldwide due to late diagnosis and limited treatment options. Studies focusing on the identification of new substances with antifungal activity, such as essential oils (EOs), are urgently needed. While the antifungal effects of EO have already been suggested, their mechanism of action at the molecular level still requires evaluation. In this work, we assessed the molecular changes induced by the exposure of Cryptococus neoformans (H99) and Cryptococcus deuterogatti (R265) to lavender essential oil (LEO) using a morphological and proteomics approach. The identified proteins were categorized by Gene Ontology according to biological processes and molecular functions, and Kyoto Encyclopedia of Genes and Genomes pathway analysis was also conducted. Our findings indicate that LEO creates a stressful environment in both strains; however, the response to this stimulus differs between the two species. In C. neoformans, changes were observed in energy metabolism and pathways related to alternative sources of energy and oxidative stress response. In C. deuterogatti, changes were identified in pathways related to cellular architecture, implying that the cell underwent morphological changes such as membrane and cell wall stiffening.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cecal microbiome in broiler chicken related to antimicrobial feeding and bird's sex. 肉鸡盲肠微生物组与抗菌饲料和鸟类性别的关系。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0190

Philip H W Mak, Xianhua Yin, Lindsey Clairmont, Lisa Bean-Hodgins, Elijah G Kiarie, Joshua Tang, Dion Lepp, Moussa S Diarra

{"title":"Cecal microbiome in broiler chicken related to antimicrobial feeding and bird's sex.","authors":"Philip H W Mak, Xianhua Yin, Lindsey Clairmont, Lisa Bean-Hodgins, Elijah G Kiarie, Joshua Tang, Dion Lepp, Moussa S Diarra","doi":"10.1139/cjm-2024-0190","DOIUrl":"10.1139/cjm-2024-0190","url":null,"abstract":"This study investigated the cecal microbiome of broilers raised under specific antimicrobial feeding programs (AFPs). A total of 2304 day-old Ross-708 male (M, n = 1152) and female (F, n = 1152) chicks were distributed into 48 floor pens which were allocated to one of three AFPs: Conventional, raised without medically important antibiotics (RWMIA), and raised without antibiotics (RWA). At 28 (D28) and 41 (D41) days of age, cecal contents were collected for culture dependent and independent analyses. At both 28 and 41 days, Enterococcus was more abundant in RWA-raised broilers than other groups with the most abundance of this bacterium being found in female birds (P < 0.05). At D41, the most abundant Eimeria tenella counts was observed in RWA-raised broiler ceca (P < 0.05). Sex effects were observed on the abundances of four of the 248 identified antimicrobial resistance genes while abundances of 10 were modulated by AFPs (P < 0.05). Ceca of females birds showed more tssB than males, and ceca of RWMIA-raised birds contained the highest abundance of chuY genes regardless of sex. This study showed that in a specific feeding program, cecal resistome can be affected by chicken's sex contributing to understand the AMR related to the AMU.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-19"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morchella capensis sp. nov., the Fynbos morel-the first description of an endemic Morchella species from Africa. 羊肚菌capensis sp. nov.， Fynbos羊肚菌-非洲特有羊肚菌的首次描述。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2023-0224

B Van der Merwe, J Williams, E Le Roux, A Van Staden, G Lawrence, K Jacobs

引用次数: 0

BEVA2.0: modular assembly of golden gate-compatible vectors with expanded utility for genetic engineering. BEVA2.0：金门兼容载体的模块化组装与扩展的基因工程实用程序。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0246

Barney A Geddes, Riley Williamson, Jake Schumacher, Ahmad Ardi, Garrett Levin, Emily Červenka, Rui Huang, George C diCenzo

引用次数: 0

Survival of antimicrobial resistant Salmonella Heidelberg inoculated into microcosms of fresh pine wood shavings for broiler litter. 接种到肉鸡粪便新鲜松木刨花微生态系统中的耐抗菌素海德堡沙门氏菌的存活率

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-22 DOI: 10.1139/cjm-2024-0088

Adelumola Oladeinde, Kimberly Cook, Attiq Rehman, Catherine D Carrillo, Reed Woyda, Crystal Wiersma, Zaid Abdo, Jasmine Johnson, Anna Marie Bosch, Michael Rothrock, Moussa S Diarra

引用次数: 0

Tolerance mechanisms and molecular epidemiology of reduced susceptibility to chlorhexidine digluconate in different species of the Acinetobacter baumannii complex. 不同种类鲍曼不动杆菌复合物对二光酸氯己定敏感性降低的耐受性机制和分子流行病学。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-12-20 DOI: 10.1139/cjm-2024-0174

Liqiong Chen, Shixing Liu, Na Huang, Chunquan Xu, Cui Zhou, Lingbo Wang, Xiangkuo Zheng, Tieli Zhou, Pingting Yu

{"title":"Tolerance mechanisms and molecular epidemiology of reduced susceptibility to chlorhexidine digluconate in different species of the Acinetobacter baumannii complex.","authors":"Liqiong Chen, Shixing Liu, Na Huang, Chunquan Xu, Cui Zhou, Lingbo Wang, Xiangkuo Zheng, Tieli Zhou, Pingting Yu","doi":"10.1139/cjm-2024-0174","DOIUrl":"10.1139/cjm-2024-0174","url":null,"abstract":"The objective of this study was to compare chlorhexidine digluconate and other antibiotics susceptibility of four species of theAcinetobacter baumannii complex, and further investigate the chlorhexidine digluconate (CHG) tolerance mechanisms and molecular epidemic characteristics. Of 889 A. baumannii complex isolates, A. baumannii, A. nosocomialis, A. pittii, and A. seifertii accounted for 84.2%, 10.9%, 3.4%, and 1.5%. Acinetobacter baumannii was generally resistant to all tested antibiotics, while other three species were commonly more susceptible; 92.1% (313/340) CHG-tolerant A. baumannii, 19.6% (19/97) CHG-tolerant A. nosocomialis, 3.3% (1/30) CHG-tolerant A. pittii, and 15.4% (2/13) CHG-tolerant A. seifertii were identified. Furthermore, compared to A. baumannii ATCC 19606, upregulated expression was found in qacEΔ1, fabI, and efflux pump encoding genes in CHG-tolerant A. baumannii, but the expression level of oprD was reduced. Additionally, only the expression level of fabI was increased in the CHG-tolerant A. nosocomialis, and the expression level of adeG was increased in the CHG-tolerant A. pittii and A. seifertii. Furthermore, CHG-tolerant A. baumannii may have a relatively high clonal correlation, the predominant sequence type of which was ST208 (90%, 36/40). It is rather necessary to identify specific species members among the A. baumannii complex for clinical treatment options and antibiotics resistance monitoring.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and distribution characteristics of myxobacteria in the rhizosphere and nonrhizosphere soils of the halophyte Haloxylon ammodendron in the high saline-alkaline Ebinur Lake Wetland. 艾比湖高盐碱湿地梭梭根际和非根际土壤黏菌多样性及分布特征

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0045

Xiaoyun Qi, Suhui Hou, Wenge Hu, Cheng Ding, Yang Li, Jie Xiong

{"title":"Diversity and distribution characteristics of myxobacteria in the rhizosphere and nonrhizosphere soils of the halophyte Haloxylon ammodendron in the high saline-alkaline Ebinur Lake Wetland.","authors":"Xiaoyun Qi, Suhui Hou, Wenge Hu, Cheng Ding, Yang Li, Jie Xiong","doi":"10.1139/cjm-2024-0045","DOIUrl":"10.1139/cjm-2024-0045","url":null,"abstract":"This study employed Illumina HiSeq high-throughput sequencing technology to analyze the V4-V5 regions of myxobacterial 16S rRNA in rhizosphere and nonrhizosphere soils of Haloxylon ammodendron in the saline-alkaline wetland of Ebinur Lake, with the aim of investigating the community structure and diversity of myxobacteria. Results indicated that myxobacterial communities in rhizosphere soils exhibited greater diversity and richness compared to nonrhizosphere soils. Soil physicochemical properties, particularly moisture content, were identified as key environmental factors influencing myxobacterial diversity. The halotolerant genus Haliangium was found to be predominant under saline-alkaline conditions. Additionally, myxobacteria demonstrated distinct ecological specificity and environmental adaptability between rhizosphere and nonrhizosphere soils. For example, the genus Enhygromyxa exhibited a negative correlation with soil moisture content in rhizosphere soils but a positive correlation with soil electrical conductivity in nonrhizosphere soils. Co-occurrence network analysis revealed complex interaction patterns among myxobacterial genera and other bacterial genera, with closer interactions observed in rhizosphere soils. This study highlights the importance of environmental factors in regulating microbial community structure and function in saline-alkaline wetlands, providing new insights into the ecological roles and interaction mechanisms of myxobacteria within the ecosystem.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"71 ","pages":"1-17"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial ecology and evolution. 微生物生态学和进化。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1139/cjm-2024-0192

Rebecca T Doyle, Leanne A Grieves, Aleeza C Gerstein

引用次数: 0

Rapid identification of Fusarium species causing head blight in Canada using MALDI-TOF mass spectrometry. 用MALDI-TOF质谱法快速鉴定加拿大引起头疫病的镰刀菌。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2025-0035

Shimosh Kurera, Bhaktiben Patel, Tehreem Ashfaq, Maria Eckhardt, Janice Bamforth, Niradha Withana Gamage, Gurcharn Singh Brar, Linda J Harris, Maria Antonia Henriquez, Rajagopal Subramaniam, Xiben Wang, Zamir Punja, Curtis Pozniak, Matthew G Bakker, Sean Walkowiak

{"title":"Rapid identification of Fusarium species causing head blight in Canada using MALDI-TOF mass spectrometry.","authors":"Shimosh Kurera, Bhaktiben Patel, Tehreem Ashfaq, Maria Eckhardt, Janice Bamforth, Niradha Withana Gamage, Gurcharn Singh Brar, Linda J Harris, Maria Antonia Henriquez, Rajagopal Subramaniam, Xiben Wang, Zamir Punja, Curtis Pozniak, Matthew G Bakker, Sean Walkowiak","doi":"10.1139/cjm-2025-0035","DOIUrl":"10.1139/cjm-2025-0035","url":null,"abstract":"Fusarium head blight is a limitation to grain production and can be caused by several different Fusarium species. We evaluated the ability of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to perform species identifications. The method generates a unique peptide mass fingerprint (PMF) for each sample that can be matched to a reference library. We first created a reference library of PMF profiles for Fusarium species from across Canada. Then, we tested the library to perform identifications using two validation panels. The first panel consisted of 820 fungal isolates from wheat (2021-2023 harvest years) and the second was 74 fungal isolates from oat and barley (2022 harvest year). The species identity of samples from the validation panels was confirmed with high-throughput quantitative PCR using species-specific DNA markers. The first validation panel was mostly F. graminearum and there was 95% overlap between the MALDI-TOF MS and DNA-based identifications. The second panel was mostly F. poae and the identifications from the two methods had 86% overlap. Our findings indicate that MALDI-TOF MS biotyping is sensitive enough to identify Fusarium strains to their species complexes and certain Fusarium strains to the species level.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0