Canadian journal of microbiology最新文献

When DNA Writing is Free: Open Tools and Strategies to Accelerate the Bioeconomy. 当DNA写作是免费的：开放的工具和策略，以加速生物经济。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-18 DOI: 10.1139/cjm-2025-0022

Benjamin Scott, Scott Pownall

{"title":"When DNA Writing is Free: Open Tools and Strategies to Accelerate the Bioeconomy.","authors":"Benjamin Scott, Scott Pownall","doi":"10.1139/cjm-2025-0022","DOIUrl":"https://doi.org/10.1139/cjm-2025-0022","url":null,"abstract":"DNA synthesis and assembly techniques have enabled the creation of validated and standardized DNA parts, used for producing proteins, enzymes, and small molecules. However, most DNA parts are governed by material transfer agreements, which restrict sharing and reuse for commercial purposes even in the absence of patents, bottlenecking innovation. DNA synthesis, crucial for producing new parts, also remains expensive and therefore inaccessible to most researchers. With the breakneck pace of digital innovations for designing and learning from biology, a new and more open approach to the physical building and testing of biology is needed. We propose the establishment of an Open Bio Research Alliance, to create and distribute open collections of DNA and other biological parts, combined with regulated and affordable DNA synthesis services. Focusing on Canada's bioeconomy, establishing domestic DNA synthesis infrastructure would not only secure global competitiveness in engineering biology, but also safeguard biosecurity and national sovereignty over critical resources. By harnessing and supporting existing lab automation resources, the Alliance will also help scale the building and testing of engineered biological systems. Leveraging these tools and strategies, Canada is well-positioned to lead the world in open and innovative biotechnology, paving the way for a thriving bioeconomy.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of aquaculture and genomic surveillance techniques on antimicrobial resistance gene profiles and microbiota detected in marine and freshwater sediments. 水产养殖和基因组监测技术对海洋和淡水沉积物中检测到的抗菌素耐药基因谱和微生物群的影响

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-16 DOI: 10.1139/cjm-2024-0206

Lisa A Johnson, Derek D N Smith, Renuka Subasinghe, Monique Raap, Robin Richter, David Huyben, Calvin Lau, Liam Brown, Jackson Chu, Olena Khomenko, Anthony Manning, Stewart Johnson, Dounia Hamoutene

{"title":"Influence of aquaculture and genomic surveillance techniques on antimicrobial resistance gene profiles and microbiota detected in marine and freshwater sediments.","authors":"Lisa A Johnson, Derek D N Smith, Renuka Subasinghe, Monique Raap, Robin Richter, David Huyben, Calvin Lau, Liam Brown, Jackson Chu, Olena Khomenko, Anthony Manning, Stewart Johnson, Dounia Hamoutene","doi":"10.1139/cjm-2024-0206","DOIUrl":"https://doi.org/10.1139/cjm-2024-0206","url":null,"abstract":"Surveillance methods for antimicrobial resistance genes (ARGs) are needed to assess potential risk of antimicrobial resistance (AMR), especially in complex environmental samples with limited data on ARG distribution. This study employed target-enrichment metagenomics (bait-capture) and a Resistomap qPCR assay to assess the resistome in marine and freshwater sediments associated with active Canadian finfish aquaculture operations. Differences in resistome profiles were considered with distance to the net-pens, concentrations of three aquaculture-associated chemical residues, and microbial communities as detected with 16S rRNA gene amplicon sequencing. With bait-capture, a total of 194 ARGs and 41 replicon types were detected across the sediment samples. Differences due to aquaculture proximity were noted in the composition of the resistome, which was dominated by tetracycline resistance genes. With qPCR, 37 out of 51 ARGs targets were detected, and proximity to net-pens or region did not show changes in resistome composition. Co-occurrence networks revealed significant correlations among genera and the resistome detected with bait-capture, highlighting a potential influence of aquaculture on ARGs in the environment. This study demonstrates the utility of bait-capture and qPCR assays in detection of ARGs in both freshwater and marine sediments from aquaculture sites that will assist further ARG surveillance.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification and community study of root fungal isolates from disturbed and undisturbed arctic and alpine tundra habitats in Northern Quebec, Canada. 加拿大魁北克北部受干扰和未受干扰北极和高山冻土带生境根真菌分离、鉴定和群落研究。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-11 DOI: 10.1139/cjm-2024-0211

Léonie Côté, Godar Sene, Marie-Eve Beaulieu, Stéphane Boudreau, Damase P Khasa

{"title":"Isolation, identification and community study of root fungal isolates from disturbed and undisturbed arctic and alpine tundra habitats in Northern Quebec, Canada.","authors":"Léonie Côté, Godar Sene, Marie-Eve Beaulieu, Stéphane Boudreau, Damase P Khasa","doi":"10.1139/cjm-2024-0211","DOIUrl":"https://doi.org/10.1139/cjm-2024-0211","url":null,"abstract":"The use of root symbionts can enhance the establishment and survival of plant communities on drastically disturbed sites. Here, we are interested in root symbionts associated with plants from the Schefferville mining site in northern Quebec. Very few studies of root fungal communities have been conducted in such a northern environment. The spatial and host plant variation of root fungal isolates was investigated. A total of 456 culturable fungi were isolated, of which 376 were successfully identified and assigned to 106 taxa based on rDNA internal transcribed spacer analysis (ITSF-1 and ITS-4). The most frequently isolated fungi belonged to three genetically related groups: the Rhizoscyphusericae aggregate (REA), the dark septate endophytes and Umbelopsisrammaniana and Mortierella sp., which are known to be genetically very close and often misclassified. These groups were present on both disturbed and natural sites, but it appears that plants from disturbed sites had a greater affinity for dark septate endophytes. This study is the first step in the development of a restoration plan for the Schefferville mining site.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional buckling model reveals the evolution of energy-driven biofilm wrinkle morphologies. 三维屈曲模型揭示了能量驱动生物膜皱折形态的演化过程。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-05-29 DOI: 10.1139/cjm-2024-0196

Jin Wu, Jin Li, Jiankun Wang, Xiaoling Wang

引用次数: 0

A β-Amino-Ketone that Disrupts the Fungal Plasma Membrane Exhibits Potent Activity Against Pathogenic Trichophyton Species. 一种破坏真菌质膜的β-氨基酮显示出对致病性毛癣菌的有效活性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-05-27 DOI: 10.1139/cjm-2025-0009

Jéssica Campos, Diego Oliveira, Bruna da Silva Souza, Lucas de Oliveira, Ezequias Siqueira, Markus Kohlhoff, Anderson Assunção Andrade

{"title":"A β-Amino-Ketone that Disrupts the Fungal Plasma Membrane Exhibits Potent Activity Against Pathogenic Trichophyton Species.","authors":"Jéssica Campos, Diego Oliveira, Bruna da Silva Souza, Lucas de Oliveira, Ezequias Siqueira, Markus Kohlhoff, Anderson Assunção Andrade","doi":"10.1139/cjm-2025-0009","DOIUrl":"https://doi.org/10.1139/cjm-2025-0009","url":null,"abstract":"Superficial fungal infections, mainly caused by dermatophytes, are a global public health issue. We evaluated the antifungal activity of six β-amino-ketones against Trichophyton rubrum, a leading agent of superficial mycoses. Among them, 3-(morpholin-4-yl)-1-phenylpropan-1-one (AB1) showed the most potent effect, with a minimum inhibitory concentration (MIC) of 7.81 µg/ml against the T. rubrum reference strain and fungicidal activity against clinical isolates, as demonstrated by minimum fungicidal concentration (MFC) assays. AB1 was effective against both conidia and hyphae of T. rubrum, while showing limited activity against Candida albicans and the bacteria Escherichia coli and Staphylococcus aureus. Mechanistic studies suggest AB1 targets the fungal plasma membrane, possibly via ergosterol interactions, supported by increased MICs in ergosterol-rich conditions and membrane integrity assays. Confocal microscopy revealed morphological alterations in AB1-treated hyphae, indicative of membrane damage. Transmission electron microscopy confirmed cytoplasmic disorganization and membrane disruption at subinhibitory concentrations. Toxicological assays showed moderate cytotoxicity in human fibroblasts (IC₅₀ = 37.75 µg/ml) and no toxicity in Galleria mellonella larvae at high doses. These findings highlight AB1 as a promising antifungal candidate against Trichophyton spp., with the potential benefit of reduced impact on the host microbiota compared to broad-spectrum antimicrobials.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of inoculation of the sap collection system with three Pseudomonadota strains on maple syrup microbial and organoleptic quality. 接种3株假单胞菌采集系统对枫糖浆微生物及感官品质的影响。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-05-23 DOI: 10.1139/cjm-2024-0225

Mérilie Gagnon, Jessica Houde, Stéphane Corriveau, Carmen Charron, Luc Lagacé

{"title":"Impact of inoculation of the sap collection system with three Pseudomonadota strains on maple syrup microbial and organoleptic quality.","authors":"Mérilie Gagnon, Jessica Houde, Stéphane Corriveau, Carmen Charron, Luc Lagacé","doi":"10.1139/cjm-2024-0225","DOIUrl":"https://doi.org/10.1139/cjm-2024-0225","url":null,"abstract":"As it flows through the collection system, maple sap is likely to be contaminated by microorganisms that colonize the tubing, potentially compromising its quality in terms of physicochemical properties, microbial load, and flavor. This study investigates the effect of microbial inoculation, as protective cultures, on the sap collection system to improve maple syrup quality. The research explored how inoculating collection tubing with specific bacterial strains influences the microbial composition, physicochemical properties (pH, Brix, conductivity, sugars and organic acids content), and sensory attributes of both maple sap and syrup. Three strains selected for their capacity to produce biofilm on plastic tubing and their impact on maple syrup production from inoculated sap, Pseudomonas sp. MSB2019, Janthinobacterium lividum 100-P12-9, and Pseudomonas fluorescens ATCC 17926, were inoculated to independent sap collection system throughout two sugaring seasons. A non-inoculated systems was included. Pseudomonas sp. MSB2019 treatment resulted in a distinct bacterial composition in sap and impact the organoleptic properties of syrup by the end of second flow season, particularly the maple and overall flavor intensity scores were higher. While sap yield and primary microbial load remained unaffected, inoculation treatments corresponded to shifts in flavor attributes of the syrup. These findings indicate that inoculating sap collection systems with targeted strains can positively influence maple syrup quality, particularly in enhancing desirable flavor profiles, suggesting promising applications for syrup production.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of MAPK Signalling and Amino Acid Biosynthesis Underlies the Antifungal Biocontrol Mechanism of Coniochaeta velutina Against Colletotrichum gloeosporioides: A Transcriptome and Metabolome Analysis. MAPK信号传导和氨基酸生物合成的中断是绒毛Coniochaeta对炭疽菌的生物防治机制的基础：转录组和代谢组分析。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-05-21 DOI: 10.1139/cjm-2023-0223

Wei Zheng, Chunling Yang, Chengbo Zhou, Tianxiao Ma, Qingsong Zhu, Zhuoqi Li

{"title":"Disruption of MAPK Signalling and Amino Acid Biosynthesis Underlies the Antifungal Biocontrol Mechanism of Coniochaeta velutina Against Colletotrichum gloeosporioides: A Transcriptome and Metabolome Analysis.","authors":"Wei Zheng, Chunling Yang, Chengbo Zhou, Tianxiao Ma, Qingsong Zhu, Zhuoqi Li","doi":"10.1139/cjm-2023-0223","DOIUrl":"https://doi.org/10.1139/cjm-2023-0223","url":null,"abstract":"This study isolated an endophytic fungus, Coniochaeta velutina, from healthy Camellia oleifera leaves and investigated its inhibitory mechanism on Colletotrichum gloeosporioides using transcriptomics and metabolomics analyses. These helped evaluate the gene expression and metabolite content differences between the treatment and control groups. The transcriptomic analysis detected a total of 15,310 expressed genes, with 3,938 showing significant differential expression (p < 0.05). These genes were primarily enriched in the following pathways: mitogen activated protein kinases (MAPK) signaling, antibiotic biosynthesis, amino acid metabolism, carbon metabolism, and peroxidase pathways. Metabolomics analysis identified 452 metabolites in both groups, with 138 showing significant differences. They were mainly enriched in secondary metabolite biosynthesis, amino acid biosynthesis, and α-linolenic acid metabolism. Transcriptome and metabolome association analysis along with qPCR results significantly increased intracellular pectolinarigenin metabolites in the treatment group, leading to disrupted MAPK signaling and reduced amino acid biosynthesis essential for maintaining normal cell growth. Notably, the decrease in terpenoid compounds was the primary reason for Colletotrichum gloeosporioides being inhibited by the biocontrol fungus Coniochaeta velutina. Therefore, these findings provide valuable insights into the biocontrol mechanisms of Coniochaeta velutina against Colletotrichum gloeosporioides and offer a promising foundation for developing new anthracnose prevention and control strategies.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of a sensitive approach for detection and recovery of third-generation cephalosporin- and carbapenem-resistant Enterobacterales from ready-to-eat frozen stone fruit. 开发和评估从即食冷冻核果中检测和回收耐第三代头孢菌素和碳青霉烯类肠杆菌的灵敏方法。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0210

Adeeb Nasta, Ashley L Cooper, Irelynd V Tackabury, Chloe Anastasiadis, Calvin Ho-Fung Lau, Liam P Brown, Myron L Smith, Sandeep Tamber, Catherine D Carrillo

{"title":"Development and evaluation of a sensitive approach for detection and recovery of third-generation cephalosporin- and carbapenem-resistant Enterobacterales from ready-to-eat frozen stone fruit.","authors":"Adeeb Nasta, Ashley L Cooper, Irelynd V Tackabury, Chloe Anastasiadis, Calvin Ho-Fung Lau, Liam P Brown, Myron L Smith, Sandeep Tamber, Catherine D Carrillo","doi":"10.1139/cjm-2024-0210","DOIUrl":"10.1139/cjm-2024-0210","url":null,"abstract":"Antimicrobial resistance (AMR) is a global public health threat, but the role of foods in its dissemination is poorly understood. We examined the incidence of foodborne bacteria carrying AMR genes considered high-priority research targets by the World Health Organization. Frozen, ready-to-eat, avocado, coconut, mango, and peach (n = 161) were tested for bacteria encoding extended-spectrum β-lactamases (ESBLs) and carbapenemases. Over 600 presumptive-positive isolates were recovered and analyzed with a pooled sequencing (Pool-seq) strategy. Coconut samples exhibited the highest bacterial loads and prevalence/diversity of AMR genes. Isolates harbouring the β-lactamase genes blactx-m, blatem, and blashv, identified in 14 coconut and 2 mango samples, were further characterized by whole-genome sequencing and antimicrobial susceptibility testing. The most common gene was blactx-m-15, detected in 20 unique strains. Two carbapenemase-producing strains were isolated from coconut: Enterobacter roggenkampii encoding blandm-1 and Escherichia coli encoding blandm-5. Subsequent quantitative PCR (qPCR) analysis of enrichments for blactx-m/blandm indicated a potentially higher prevalence of these genes than observed by colony screening. This study presents a practical method for recovering ESBL- and carbapenemase-producing bacteria from foods. Mapping their distribution in food products is crucial to assessing the role of foods in the global spread of AMR and developing effective public health interventions.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-18"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between common scab-inducing strains on potato suberin. 马铃薯单宁上常见疮痂诱导菌株之间的相互作用。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0242

Mario Khalil, Iauhenia Isayenka, Rebeca Padilla-Reynaud, Sylvain Lerat, Carole Beaulieu

{"title":"Interactions between common scab-inducing strains on potato suberin.","authors":"Mario Khalil, Iauhenia Isayenka, Rebeca Padilla-Reynaud, Sylvain Lerat, Carole Beaulieu","doi":"10.1139/cjm-2024-0242","DOIUrl":"10.1139/cjm-2024-0242","url":null,"abstract":"Potato tuber periderm is armored with suberin, that consists of two domains, an aliphatic domain composed of fatty acid polyesters and an aromatic domain composed of cinnamic acids. Streptomyces scabies 87.22, a predominant causal agent of potato common scab, was compared for adaptation to tuber suberin with Streptomyces acidiscabies ATCC 49003 and Streptomyces turgidiscabies Car8 belonging to emerging pathogenic species. Streptomyces scabies 87.22 showed higher growth in the suberin supplemented medium than the two other strains. When co-cultured in a rich nutrient medium, S. acidiscabies ATCC 49003 produced the antibiotic oxanthromicin, which inhibited growth and mycelium development of the other strains. Exposure of S. scabies 87.22 and S. acidiscabies ATCC 49003 to suberin was accompanied by the secretion of enzymes degrading cellulose, hemicellulose, fatty acids, and glycerol derivatives. Compared to the two other strains, S. scabies 87.22 showed higher esterase activity in suberin-supplemented medium and strong induction of cellulase gene expression. Both S. acidiscabies ATCC 49003 and S. turgidiscabies Car8 exhibited a poor utilization of trans-ferulic and p-coumaric acids, suggesting almost no ability to degrade the aromatic moiety of suberin. This work suggests that S. scabies 87.22 is better adapted to the potato periderm degradation than the emerging pathogens. The elucidation of pathogenic Streptomyces strains interaction may contribute to the improvement of ecologically oriented agronomic strategies for common scab management.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-15"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Characterization and whole-genome sequencing of an extreme arsenic tolerant Citrobacter freundii SRS1 strain isolated from Savar area in Bangladesh. 更正：从孟加拉国Savar地区分离的一种极端耐砷弗氏柠檬酸杆菌SRS1菌株的鉴定和全基因组测序。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-12-17 DOI: 10.1139/cjm-2024-0195

Mohammed Jafar Uddin, Farhana Haque, Ishrat Jabeen, Sabbir R Shuvo

引用次数: 0