Canadian journal of microbiology最新文献

A One Health genomic assessment of Salmonella enterica across dairy, poultry, and environmental reservoirs in British Columbia, Canada. 加拿大不列颠哥伦比亚省乳制品、家禽和环境水库中肠沙门氏菌的One Health基因组评估。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-10-06 DOI: 10.1139/cjm-2026-0143

Sandeep Tamber, Shawn Hoogstra, Cassandra Klaas, Annika Flint, Kelly Weedmark, Mary Rao, David Mahoney, Daniella Rizzo, Richard Reid-Smith, Rhiannon Louise Wallace

{"title":"A One Health genomic assessment of Salmonella enterica across dairy, poultry, and environmental reservoirs in British Columbia, Canada.","authors":"Sandeep Tamber, Shawn Hoogstra, Cassandra Klaas, Annika Flint, Kelly Weedmark, Mary Rao, David Mahoney, Daniella Rizzo, Richard Reid-Smith, Rhiannon Louise Wallace","doi":"10.1139/cjm-2026-0143","DOIUrl":"https://doi.org/10.1139/cjm-2026-0143","url":null,"abstract":"Salmonella enterica is an important zoonotic pathogen affecting animal and human health. Using a One Health approach, we conducted a comparative genomic analysis of S. enterica recovered within a restricted peri-urban region in Western Canada consisting of six dairy farms, an organic poultry farm, surrounding environments, and municipal wastewater. Overall, Salmonella prevalence was low, with 34 of 1,004 samples (3.4%) testing positive and representing 11 serovars. Antimicrobial resistance was limited: all isolates were susceptible to 12 of 14 antimicrobials tested, with resistance detected only to streptomycin (35%) and tetracycline (12%). Although all isolates carried the cryptic aminoglycoside resistance gene aac(6')-Iaa and 65% harboured the parC T57S mutation, neither determinant was associated with phenotypic resistance. Serovar Kentucky was the most widely distributed, occurring in dairy, poultry, and environmental samples. Plasmid profiles indicated genomic linkages among isolates from dairy farms, poultry, and the environment. Phylogenomic analyses revealed genetic similarities across animal and environmental sectors, while comparison with publicly available S. enterica ser.Enteritidis genomes identified a potential linkages between poultry and dairy production systems. These findings highlight the low prevalence of Salmonella in the study region and the value of One Health surveillance for identifying reservoirs, transmission pathways, and opportunities to strengthen biosecurity.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coincidental observation of a parvovirus during a targeted bacterial survey of cattle suffering from septic arthritis. 在对患败血性关节炎的牛进行有针对性的细菌调查时，偶然观察到一种细小病毒。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-09-24 DOI: 10.1139/cjm-2026-0033

Daniel Kos, Ashton N Sies, Murray Jelinski, Andrew D S Cameron, Antonio Ruzzini

{"title":"Coincidental observation of a parvovirus during a targeted bacterial survey of cattle suffering from septic arthritis.","authors":"Daniel Kos, Ashton N Sies, Murray Jelinski, Andrew D S Cameron, Antonio Ruzzini","doi":"10.1139/cjm-2026-0033","DOIUrl":"https://doi.org/10.1139/cjm-2026-0033","url":null,"abstract":"DNA capture-based sequencing by hybridization probe enrichment (CapSeq) affords high-throughput and targeted gene-to-organism detection. Despite the specificity designed into CapSeq platforms, untargeted DNA represent additional genes and organisms of interest. To this end, de novo metagenome assemblies of CapSeq datasets present opportunities for discovery. We report an example here. CapSeq of samples that originated from cattle suffering from septic arthritis resulted in detection and assembly of two ungulate tetraparvovirus 1 (UTPV1) genomes. These coincidental detections and assemblies were obtained from a platform designed to characterize bacterial, not viral, pathogens. Access to a sample matched shotgun metagenomic dataset corroborated the result. Importantly, septic arthritis is a significant source of lameness in beef cattle that lacks effective and approved treatments, and the list of aetiological agents is incomplete. This metagenomic observation calls attention to the possible implication of viral contributors to bovine septic arthritis, which is treated as a bacterial disease. The results exemplify the potential application of unbiased CapSeq data mining to support clinical discoveries.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting growth responses of early- and late-successional native grasses to AMF-rich whole soil inocula under greenhouse conditions. 温室条件下早、晚演替原生禾草对富amf全土接种物的生长响应对比

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-27 DOI: 10.1139/cjm-2026-0099

Martina Cardoni, Janelle Card, Jonathan A Bennett

{"title":"Contrasting growth responses of early- and late-successional native grasses to AMF-rich whole soil inocula under greenhouse conditions.","authors":"Martina Cardoni, Janelle Card, Jonathan A Bennett","doi":"10.1139/cjm-2026-0099","DOIUrl":"https://doi.org/10.1139/cjm-2026-0099","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) form symbioses in terrestrial ecosystems, yet plant responses to AMF-rich soil inocula are variable and do not necessarily reflect root colonization. We investigated how plant successional strategy and AMF-rich soil inocula interact to drive plant performance and root colonization structures. In a greenhouse experiment, four early- and late-successional native grasses were grown with five trap-cultured AMF-rich whole soil inocula. We assessed responses in biomass and height, expressed as log response ratios relative to sterile controls, alongside intraradical hyphal and arbuscular colonization. Biomass responses were generally negative and depended on the interaction between inoculum treatment and successional group. Late-successional species exhibited less negative growth responses than early-successional species. The probability of detecting hyphal colonization differed among inoculum treatments, whereas arbuscular colonization depended on the interaction between inoculum and successional group. However, neither arbuscules nor hyphal colonization predicted biomass responses, showing that greater physical colonization does not necessarily translate into greater plant growth. These findings show that structural colonization alone is insufficient to predict the growth effects of AMF-rich whole soil inocula. For grassland restoration, our results highlight that evaluating soil inocula based solely on physical colonization is insufficient; successful management requires assessing their functional effects on target native grasses.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Report on High Pathogenicity Avian Influenza in Antarctica and sub-Antarctic Islands. 关于南极洲和亚南极岛屿高致病性禽流感的全球报告。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-06-23 DOI: 10.1139/cjm-2025-0293

Meagan Dewar, Michelle Wille

引用次数: 0

Bacterial changes and quality deterioration of freshwater shellfish Hyriopsis cumingii meat under different temperature storage. 不同贮藏温度下淡水贝三角帆蚌肉的细菌变化及品质劣化

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0056

Yuntao Li, Huan Li, Chaotian Lv, Xinyuan Hu, Bin Zhang

{"title":"Bacterial changes and quality deterioration of freshwater shellfish Hyriopsis cumingii meat under different temperature storage.","authors":"Yuntao Li, Huan Li, Chaotian Lv, Xinyuan Hu, Bin Zhang","doi":"10.1139/cjm-2025-0056","DOIUrl":"10.1139/cjm-2025-0056","url":null,"abstract":"Hyriopsis cumingii is an important economic freshwater shellfish in China and there is a need to understand changes in the microbial community structure resulting in multidimensional quality degradation when the fish is stored at different temperatures. This study integrated 16S rRNA full-length sequencing with multidimensional quality indicators to investigate the temperature-regulated bacterial community shifts and quality deterioration mechanisms in stored H. cumingii meat. The results showed that bacterial richness (Chao1 index) decreased progressively with both refrigerated (4 °C) and room-temperature (25 °C) storage. Community composition underwent significant restructuring, with Bacteroidota decreasing at 25 °C while Bacillota increased compared to 4 °C storage. Additionally, the refrigerated group showed enrichment of Delftia turuhatensis and Chryseobacterium indologenes compared to the room-temperature storage group. Temperature significantly restructured bacterial communities, with notably higher pathogenic bacteria under refrigeration and spoilage bacteria dominance at room temperature. Metagenomic functional profiling revealed temperature-driven metabolic pathway divergence, indicating distinct spoilage mechanism. Predictable quality changes in H. cumingii correlated with temperature-imposed microbial composition.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological control of the soil-borne fungal pathogen Fusarium oxysporum f. sp. lycopersici-a review. 土传真菌病原尖孢镰刀菌的生物防治。番茄——综述。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0232

Vandana Anand, Udit Yadav

{"title":"Biological control of the soil-borne fungal pathogen Fusarium oxysporum f. sp. lycopersici-a review.","authors":"Vandana Anand, Udit Yadav","doi":"10.1139/cjm-2025-0232","DOIUrl":"10.1139/cjm-2025-0232","url":null,"abstract":"Tomato (Solanum lycopersicum) is one of the most widely cultivated vegetables worldwide, yet its productivity is severely constrained by Fusarium wilt caused by FOL. The pathogen invades the root system and vascular tissues, leading to systemic wilting, plant collapse, and significant yield losses. Although chemical fungicides have been extensively used for management, their long-term effectiveness is declining due to the emergence of fungicide-resistant strains and the associated environmental and health hazards. This growing challenge underscores the urgent need for sustainable alternatives. In this review, we critically synthesize recent advances in the biological control of FOL, focusing on antagonistic fungi, beneficial bacteria, and the role of organic amendments in creating suppressive soils. Unlike earlier reviews that address these components separately, we emphasize their integration within holistic disease management frameworks. We also highlight promising directions, including microbial consortia, molecular insights into pathogen-antagonist interactions, and the potential of combining biological control with precision agriculture tools. Collectively, these strategies offer a sustainable pathway for mitigating Fusarium wilt and ensuring resilient tomato production systems.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145573101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome analysis of Staphylococcus caprae indicates potential health risks associated with antimicrobial resistance and virulence factors. 卡普拉葡萄球菌的基因组分析表明与抗菌素耐药性和毒力因素相关的潜在健康风险。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2026-0024

Jingjing Zhang, Xuehai Dong, Zhaoyang Zeng, Yuhui Du

{"title":"Genome analysis of Staphylococcus caprae indicates potential health risks associated with antimicrobial resistance and virulence factors.","authors":"Jingjing Zhang, Xuehai Dong, Zhaoyang Zeng, Yuhui Du","doi":"10.1139/cjm-2026-0024","DOIUrl":"https://doi.org/10.1139/cjm-2026-0024","url":null,"abstract":"Staphylococcus caprae is an emerging coagulase-negative staphylococcal pathogen. This study performed pan-genome analysis to comprehensively characterize the genomic landscape of S. caprae. Phylogenomic reconstruction confirmed that it forms a distinct monophyletic clade from closely related species (Staphylococcus epidermidis and Staphylococcus capitis). Pan-genome analysis revealed an open genome (γ = 0.149 according to Heap's law) comprising 3967 gene families, 53.5% of which constitute the core genome enriched in essential metabolic functions. Cloud gene families showed enrichment in defense mechanisms and traits associated with genomic plasticity. A total of 17 antimicrobial resistance (AMR) genes were identified, most of which were scattered sporadically across S. caprae genomes in the form of cloud genes, which indicates horizontal gene transfer. The coexistence of multiple resistance determinants (e.g., mecA, blaZ, erm(A)) could potentially lead to the development of high-risk multidrug-resistant phenotypes, which would severely limit the available therapeutic options. Virulence genotypic profiling revealed conserved pathogenic mechanisms, including the complete icaADBC operon (involved in biofilm formation), a type VII secretion system and iron acquisition systems (isd). These findings provide a pan-genome-level view of S. caprae and highlight its potential role as a reservoir of AMR genes and conserved virulence-related traits.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"72 ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azospirillum brasilense and tomato exudate or cytidine increase phytopathogen resistance and modulate phyllosphere/rhizosphere. 巴西固氮螺旋菌和番茄胞苷增加植物病原菌抗性，调节根际/根际。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2026-0032

Janice N A Tagoe, Bidya Ojha, Shelley M Horne, Birgit M Prüß

{"title":"Azospirillum brasilense and tomato exudate or cytidine increase phytopathogen resistance and modulate phyllosphere/rhizosphere.","authors":"Janice N A Tagoe, Bidya Ojha, Shelley M Horne, Birgit M Prüß","doi":"10.1139/cjm-2026-0032","DOIUrl":"10.1139/cjm-2026-0032","url":null,"abstract":"Tomatoes are an important crop worldwide and phytopathogens can cause devastating losses. This study describes a treatment, consisting of Azospirillum brasilense Sp7 and either tomato seedling exudate or the exudate compound cytidine. The combination of A. brasilense Sp7 with cytidine showed a remarkable reduction of 83.4% in disease severity of tomatoes challenged with Pseudomonas syringae pv. tomato DC3000. Replacing cytidine with exudate was less effective at 71%, but the reduction in disease severity was still larger than by A. brasilense Sp7 alone at 55%. This reduction in disease severity was not paralleled by a decrease in P. syringae in leaf homogenates. Cytidine caused a 6.7 fold increase in A. brasilense Sp7 16S rDNA in root homogenates. In phyllosphere and rhizosphere, treatments modulated the microbial composition. In the phyllosphere, specific associations between treatment groups and bacterial orders could be computed. In the rhizosphere, principal component analysis revealed that variation along PC1 was dominated by the presence or absence of A. brasilense. Intriguingly, the inoculant caused an increase in the abundance of other Azospirillales species.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating how infectious bursal disease virus influences influenza A virus transmission in chickens. 评估传染性法氏囊病病毒如何影响甲型流感病毒在鸡中的传播。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0208

P Bonney, M R Culhane, A Broadbent, C Flores-Figueroa, J Munoz-Aguayo, Kristelle Mendoza, C J Cardona

{"title":"Evaluating how infectious bursal disease virus influences influenza A virus transmission in chickens.","authors":"P Bonney, M R Culhane, A Broadbent, C Flores-Figueroa, J Munoz-Aguayo, Kristelle Mendoza, C J Cardona","doi":"10.1139/cjm-2025-0208","DOIUrl":"10.1139/cjm-2025-0208","url":null,"abstract":"Infectious bursal disease virus (IBDV) is a major cause of immunosuppression in chickens that has been shown to affect influenza A virus (avian influenza virus (AIV)) pathogenicity and shedding patterns. In this study, we compared the shedding of a low passage duck origin AIV in two groups of specific- pathogen free chickens: one group that was IBDV-inoculated and one sham-inoculated group. At 3 weeks of age, the birds were divided and intranasally given one of three doses (102, 103, 105) of A/mallard/OH/421/1987 (H7N8) low pathogenicity AIV. Although the bursae of the IBDV-infected chicks had higher lesion scores, the damage was minimal. We observed that this low IBDV exposure increased resistance to AIV challenge. Uninoculated contacts co-housed with the treatment groups given 105 AIV became infected. Additionally, chicks shedding approximately 103-4 EID50/ml AIV (CT of 33 or less) transmitted virus to cage mates. These findings demonstrate that the impacts of immunosuppressive agents on AIV challenge may vary, based on a number of confounding factors.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-7"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crop domestication, selective breeding, and the seed microbiome: a call for further research. 作物驯化、选择性育种和种子微生物组：需要进一步研究。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2026-0018

Gracie C Farago, Rafael D Grajales, Christopher K Yost

{"title":"Crop domestication, selective breeding, and the seed microbiome: a call for further research.","authors":"Gracie C Farago, Rafael D Grajales, Christopher K Yost","doi":"10.1139/cjm-2026-0018","DOIUrl":"10.1139/cjm-2026-0018","url":null,"abstract":"Plants have been domesticated for thousands of years and subject to human-derived selection for desirable traits such as improved yield, disease tolerance, nutrient content, and palatability. Advancements in high-throughput DNA sequencing advancements have allowed researchers to study the diversity of the seed microbiome. We reviewed the literature and identified articles that investigated the impact of domestication on seed microbiome diversity of various plant species. The resultant analysis suggests that the impacts of domestication and selective breeding on seed microbiome diversity are variable and inconclusive due to the low number of independent studies per species and the limited diversity of domesticated species examined. Based upon our analysis we suggest a need for standardized methodology and bioinformatic analysis to accompany further research on seed development of domesticated crop species. Understanding the mechanisms that influence plant-microbe interactions, such as microbial colonization during seed development, and their applications in sustainable crop improvement is the next step towards innovative and scalable advances in agricultural practice.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0