Canadian journal of microbiology最新文献

Host-specific soil oomycete communities are spatially repeatable across natural grasslands. 寄主特有的土壤卵菌群落在自然草原上具有空间可重复性。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-09-04 DOI: 10.1139/cjm-2026-0106

Jack Walter Lewis Royle, Itaii Applebaum, John Paul Wasan, Sean R Asselin, Jonathan A Bennett

{"title":"Host-specific soil oomycete communities are spatially repeatable across natural grasslands.","authors":"Jack Walter Lewis Royle, Itaii Applebaum, John Paul Wasan, Sean R Asselin, Jonathan A Bennett","doi":"10.1139/cjm-2026-0106","DOIUrl":"https://doi.org/10.1139/cjm-2026-0106","url":null,"abstract":"Soil oomycetes contain some of the most devastating plant pathogens, and are widespread within the soil microbiome, yet how individual plant species structure oomycete communities in natural soils, and whether any such structuring is consistent across space, remains poorly resolved. Because host-specific pathogen accumulation is a central prediction of plant-soil feedback theory but is rarely tested outside greenhouse conditions, we focused on whether the host-specific fraction of these communities is preferentially pathogenic. Using oomycete-specific ITS amplicons, we sequenced rhizosphere-associated and paired bulk soils from four grassland species across 25 sites on the Canadian Prairies spanning more than 200,000 km², totalling 1800 samples. Each host supported a distinctive core of taxa, with two species more likely to host pathogenic taxa unique to their rhizosphere. This enrichment was decoupled from richness of oomycete taxa in the rhizosphere, indicating selective accumulation rather than a by-product of resource-rich environments. Community composition also differed among hosts after accounting for site level edaphic variation. Host plant identity therefore leaves a detectable, spatially repeatable imprint on soil oomycete communities, one expressed most strongly through the selective accumulation of pathogenic taxa.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The blue light receptor gene PoWC-1 plays a role in the formation of Pleurotus ostreatus primordia. 蓝光受体基因PoWC-1在平菇原基形成中起作用。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-31 DOI: 10.1139/cjm-2026-0052

Xiaojuan Hou, Wenfeng Xie, Ruyi Huang, Mingyu Jia, Fengqin Wang, Qing Wen, Yanru Hu, Qing Liu, Jinwen Shen, Yuancheng Qi

{"title":"The blue light receptor gene PoWC-1 plays a role in the formation of Pleurotus ostreatus primordia.","authors":"Xiaojuan Hou, Wenfeng Xie, Ruyi Huang, Mingyu Jia, Fengqin Wang, Qing Wen, Yanru Hu, Qing Liu, Jinwen Shen, Yuancheng Qi","doi":"10.1139/cjm-2026-0052","DOIUrl":"https://doi.org/10.1139/cjm-2026-0052","url":null,"abstract":"Blue light is a critical environmental signal that triggers fruiting body initiation in mushroom-forming fungi. Pleurotus ostreatus is a widely cultivated model mushroom, yet the molecular mechanisms linking light perception to primordia formation remain poorly understood. Here, we functionally characterized the blue light receptor gene PoWC-1, a homolog of Neurospora crassa white collar 1, during early morphogenesis. Antisense-mediated silencing of PoWC-1 caused a marked delay in primordia formation under light/dark cycles and complete arrest at the undifferentiated mycelial stage under continuous darkness, underscoring its role in developmental progression. Even in darkness, PoWC-1 silencing downregulated key developmental transcription factors, including hom1, hom2, Pofst3, and fst4, suggesting that PoWC-1 may contribute to developmental competence independently of light. Comparative transcriptomics further revealed that PoWC-1 influences a range of metabolic pathways-such as glycolysis/gluconeogenesis, the pentose phosphate pathway, and amino sugar metabolism-that may support the energetic and biosynthetic demands of fruiting initiation. In addition, PoWC-1 appears to affect upstream signaling components, including the MAPK pathway and the two-component system, along with a suite of downstream transcription factors. These findings suggest that PoWC-1 functions as a key regulator integrating blue light signals into a multi-layered network involved in metabolic reprogramming, signal transduction, and transcriptional regulation during primordia formation in P. ostreatus. Collectively, this study provides a molecular framework for understanding photomorphogenesis in edible mushrooms.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting growth responses of early- and late-successional native grasses to AMF-rich whole soil inocula under greenhouse conditions. 温室条件下早、晚演替原生禾草对富amf全土接种物的生长响应对比

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-27 DOI: 10.1139/cjm-2026-0099

Martina Cardoni, Janelle Card, Jonathan A Bennett

{"title":"Contrasting growth responses of early- and late-successional native grasses to AMF-rich whole soil inocula under greenhouse conditions.","authors":"Martina Cardoni, Janelle Card, Jonathan A Bennett","doi":"10.1139/cjm-2026-0099","DOIUrl":"https://doi.org/10.1139/cjm-2026-0099","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) form symbioses in terrestrial ecosystems, yet plant responses to AMF-rich soil inocula are variable and do not necessarily reflect root colonization. We investigated how plant successional strategy and AMF-rich soil inocula interact to drive plant performance and root colonization structures. In a greenhouse experiment, four early- and late-successional native grasses were grown with five trap-cultured AMF-rich whole soil inocula. We assessed responses in biomass and height, expressed as log response ratios relative to sterile controls, alongside intraradical hyphal and arbuscular colonization. Biomass responses were generally negative and depended on the interaction between inoculum treatment and successional group. Late-successional species exhibited less negative growth responses than early-successional species. The probability of detecting hyphal colonization differed among inoculum treatments, whereas arbuscular colonization depended on the interaction between inoculum and successional group. However, neither arbuscules nor hyphal colonization predicted biomass responses, showing that greater physical colonization does not necessarily translate into greater plant growth. These findings show that structural colonization alone is insufficient to predict the growth effects of AMF-rich whole soil inocula. For grassland restoration, our results highlight that evaluating soil inocula based solely on physical colonization is insufficient; successful management requires assessing their functional effects on target native grasses.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptidylarginine Deiminase Inhibitor Treatment is Associated with Increased Phage Susceptibility in Erwinia amylovora. 肽精氨酸脱亚胺酶抑制剂治疗与淀粉状欧文菌噬菌体敏感性增加有关。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-26 DOI: 10.1139/cjm-2026-0025

Nassereldin Ibrahim, Antonet M Svircev, Hany Anany, Joel Weadge

引用次数: 0

SynBio 8.0: A Portrait of a Growing Canadian Ecosystem. SynBio 8.0：一个成长中的加拿大生态系统的肖像。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-24 DOI: 10.1139/cjm-2026-0122

Jeremy Gagnon, Caitlin Oh, Aracely Maribel Diaz-Garza

{"title":"SynBio 8.0: A Portrait of a Growing Canadian Ecosystem.","authors":"Jeremy Gagnon, Caitlin Oh, Aracely Maribel Diaz-Garza","doi":"10.1139/cjm-2026-0122","DOIUrl":"https://doi.org/10.1139/cjm-2026-0122","url":null,"abstract":"The 8th edition of the Annual Canadian Synthetic Biology Symposium (SynBio 8.0) was hosted at the University of Waterloo from June 15 to 17th. It brought together around 140 attendees from across Canada, as well as international participants. Organized by the Waterloo Centre for Microbial Research (WCMR) alongside SynBio Canada, SynBio 8.0 captured both the range of the work underway and the energy running through Canada's synthetic biology community. Across the scientific program, topics moved from biomanufacturing and enzyme engineering to environmental microbiology, new tool development, and plant synthetic biology. Several keynote talks and panel discussions on practical issues were the center of the conference. Trainees learned about intellectual property, key aspects of starting companies, and how scientists can better communicate their work. This edition was marked by record-setting poster numbers, serving as a clear signal that the field is accelerating the pace in Canada. Here we gathered the main viewpoints that surfaced over the three days, offering a snapshot of a discipline that is increasingly varied, confident, and established nationwide.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vanillin derivatives as antifungal agents against Aspergillus fumigatus: in vitro activity, in vivo efficacy, and mechanistic insights. 香兰素衍生物作为抗烟曲霉的抗真菌剂：体外活性，体内功效和机制的见解。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-19 DOI: 10.1139/cjm-2025-0295

Orlando Esau Flores Maldonado, Karla De Anda-Mora, Jimena G Jiménez-Barrientos, Jorge Dávila-Aviña, Valeria Muñiz-Bernal, Ana Laura Ríos López

{"title":"Vanillin derivatives as antifungal agents against Aspergillus fumigatus: in vitro activity, in vivo efficacy, and mechanistic insights.","authors":"Orlando Esau Flores Maldonado, Karla De Anda-Mora, Jimena G Jiménez-Barrientos, Jorge Dávila-Aviña, Valeria Muñiz-Bernal, Ana Laura Ríos López","doi":"10.1139/cjm-2025-0295","DOIUrl":"https://doi.org/10.1139/cjm-2025-0295","url":null,"abstract":"The World Health Organization includes Aspergillus fumigatus on the list of fungal pathogens of critical priority for the development of new therapeutic options, due to the emergence of antifungal-resistant strains and high mortality rates. This work evaluated the antifungal activity of vanillin, o-vanillin, ethyl vanillin and vanillin acetate against A. fumigatus. Broth microdilution assays were performed to determine the minimum inhibitory concentrations of the phenolic compounds. Moreover, the ability of vanillin and its derivatives to inhibit conidial adhesion and biofilm formation at subinhibitory concentrations was assessed. The possible mechanism of action of the compounds by molecular docking was determined. Finally, the therapeutic efficacy of the molecules was analyzed in an in vivo aspergillosis model. Vanillin and its derivatives exhibited antifungal effects against A. fumigatus at concentrations of 0.25 to 1 mM; notably, o-vanillin showed higher antifungal activity (0.25 mM). Furthermore, vanillin, o-vanillin and vanillin acetate reduced conidial adhesion, and biofilm formation at subinhibitory concentrations. These compounds decreased ergosterol concentration, and in silico analysis showed that phenolic compounds interact with ERG5, ERG6 and ERG11. Vanillin and o-vanillin demonstrated therapeutic efficacy against aspergillosis model. Overall, these results highlight the antifungal activity of vanillin and o-vanillin against A. fumigatus as a potential therapeutic alternative.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic Suppression of Bacillus cereus Virulence: Growth Kinetics, Cereulide Quantification, and PlcR-PapR Pathway Disruption under Lacticaseiibacillus casei, Lactiplantibacillus plantarum, and Consortia Coculture Conditions. 益生菌对蜡样芽孢杆菌毒力的抑制：在干酪乳杆菌、植物乳杆菌和菌群共培养条件下的生长动力学、Cereulide定量和PlcR-PapR通路破坏。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-18 DOI: 10.1139/cjm-2026-0087

Himani Parihar, Priyanka Chaturvedi, Ranu Dayal, Rimjhim Pathak, Prem Saran Tirumalai

{"title":"Probiotic Suppression of Bacillus cereus Virulence: Growth Kinetics, Cereulide Quantification, and PlcR-PapR Pathway Disruption under Lacticaseiibacillus casei, Lactiplantibacillus plantarum, and Consortia Coculture Conditions.","authors":"Himani Parihar, Priyanka Chaturvedi, Ranu Dayal, Rimjhim Pathak, Prem Saran Tirumalai","doi":"10.1139/cjm-2026-0087","DOIUrl":"https://doi.org/10.1139/cjm-2026-0087","url":null,"abstract":"Bacillus cereus emetic poisoning is mediated by cereulide, a thermostable cyclic depsipeptide encoded by the cesA/cesB operon under PlcR-PapR control. Once formed, cereulide resists heat and standard food processing, making transcriptional intervention the only viable biocontrol strategy. Lacticaseibacillus casei NCIM 2125, Lactiplantibacillus plantarum NCIM 2083, and a three-strain consortia additionally containing Bifidobacterium bifidum NCIM 5697 were co-cultured with B. cereus NCIM 2217 in BHI broth (1:1 v/v; OD₆₀₀ = 0.1; 37 °C) over 24-72 h (n = 3). Viable counts were determined by standard plate count, cereulide by LC-MS following liquid-liquid extraction, and cesA/cesB transcripts by RT-qPCR (2⁻ΔΔCT, dual housekeeping-gene normalisation). L. casei inhibited growth at 24 h only (1.97 log CFU/mL; p = 0.021). The consortia achieved 18.0% area-under-curve reduction and 81.6% cereulide suppression at 48 h (p < 0.001; Cohen's d = 29.03). A protein-mRNA paradox emerged: L. plantarum suppressed cesA by 87.9% at 48 h yet cereulide rose 10.5% above control (p = 0.007), attributable to depsipeptide stability post-secretion. Consortium ces silencing at 24 h (cesA FC = 0.0031) preceded peak synthesis, yielding a 77.5 percentage-point synergy bonus. Effective cereulide biocontrol requires ces silencing before the synthesis window closes, a criterion met only by the consortia.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Klebsiella sp. IR6, an endophytic rhizobacteria with potential as a plant growth-promoting agent for Jalapeño pepper (Capsicum annum L.) production. 克雷伯氏菌（Klebsiella sp. IR6）是一种内生根瘤菌，有潜力成为Jalapeño辣椒（Capsicum annum L.）生产的植物生长促进剂。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-08-14 DOI: 10.1139/cjm-2025-0311

Dulce Hernández-Luna, Ignacio Eduardo Maldonado-Mendoza, Alicia Fierro-Coronado, Francisco Roberto Quiroz-Figueroa, Jesús Eduardo Cazares-Álvarez, Rubén Félix-Gastelum, Rosa Maria Longoria-Espinoza

{"title":"Klebsiella sp. IR6, an endophytic rhizobacteria with potential as a plant growth-promoting agent for Jalapeño pepper (Capsicum annum L.) production.","authors":"Dulce Hernández-Luna, Ignacio Eduardo Maldonado-Mendoza, Alicia Fierro-Coronado, Francisco Roberto Quiroz-Figueroa, Jesús Eduardo Cazares-Álvarez, Rubén Félix-Gastelum, Rosa Maria Longoria-Espinoza","doi":"10.1139/cjm-2025-0311","DOIUrl":"https://doi.org/10.1139/cjm-2025-0311","url":null,"abstract":"Plant growth-promoting rhizobacteria (PGPR) represent a sustainable alternative to reduce excessive agrochemical use and strengthen environmentally responsible agricultural practices. This study aimed to isolate, identify, and molecularly characterize endophytic bacteria associated with jalapeño pepper (Capsicum annuum L.) to evaluate their potential as PGPR and their antagonistic activity against phytopathogenic fungi. In vitro assays were conducted to determine functional traits related to plant growth promotion and biocontrol. Phylogenetic analysis identified the genera Stenotrophomonas, Pseudomonas, Paenibacillus, Klebsiella, Aeromonas, and Enterobacter. Among these isolates, Klebsiella sp. IR6 exhibited the most notable agronomic performance, increasing seedling leaf area (57%), fruit production (89%), and fruit size (15% in length and 31% in diameter) under field conditions. Additionally, IR6 was non-hemolytic, suggesting low risk to human health. This strain demonstrated antagonistic activity against Fusarium oxysporum (15-34%), siderophore production, and phosphate solubilization, confirming its potential as a biocontrol agent and PGPR. Transformation with plasmid pAD4325 carrying the GFP gene, followed by confocal microscopy, revealed its endophytic colonization within root tissues. Further evaluations using different jalapeño hybrids are necessary to validate the biotechnological potential of IR6 for sustainable agriculture.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Report on High Pathogenicity Avian Influenza in Antarctica and sub-Antarctic Islands. 关于南极洲和亚南极岛屿高致病性禽流感的全球报告。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-06-23 DOI: 10.1139/cjm-2025-0293

Meagan Dewar, Michelle Wille

引用次数: 0

Bacterial changes and quality deterioration of freshwater shellfish Hyriopsis cumingii meat under different temperature storage. 不同贮藏温度下淡水贝三角帆蚌肉的细菌变化及品质劣化

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0056

Yuntao Li, Huan Li, Chaotian Lv, Xinyuan Hu, Bin Zhang

{"title":"Bacterial changes and quality deterioration of freshwater shellfish Hyriopsis cumingii meat under different temperature storage.","authors":"Yuntao Li, Huan Li, Chaotian Lv, Xinyuan Hu, Bin Zhang","doi":"10.1139/cjm-2025-0056","DOIUrl":"10.1139/cjm-2025-0056","url":null,"abstract":"Hyriopsis cumingii is an important economic freshwater shellfish in China and there is a need to understand changes in the microbial community structure resulting in multidimensional quality degradation when the fish is stored at different temperatures. This study integrated 16S rRNA full-length sequencing with multidimensional quality indicators to investigate the temperature-regulated bacterial community shifts and quality deterioration mechanisms in stored H. cumingii meat. The results showed that bacterial richness (Chao1 index) decreased progressively with both refrigerated (4 °C) and room-temperature (25 °C) storage. Community composition underwent significant restructuring, with Bacteroidota decreasing at 25 °C while Bacillota increased compared to 4 °C storage. Additionally, the refrigerated group showed enrichment of Delftia turuhatensis and Chryseobacterium indologenes compared to the room-temperature storage group. Temperature significantly restructured bacterial communities, with notably higher pathogenic bacteria under refrigeration and spoilage bacteria dominance at room temperature. Metagenomic functional profiling revealed temperature-driven metabolic pathway divergence, indicating distinct spoilage mechanism. Predictable quality changes in H. cumingii correlated with temperature-imposed microbial composition.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0