Canadian journal of microbiology最新文献

Exploring the Multifaceted Roles of Trichoderma Secondary Metabolites. 探索木霉次生代谢产物的多方面作用。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-07-15 DOI: 10.1139/cjm-2025-0045

Kavita Yadav, Prashant Khare

引用次数: 0

Harnessing Synthetic Biology to Empower a Circular Plastics Economy. 利用合成生物学促进循环塑料经济。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-07-10 DOI: 10.1139/cjm-2025-0053

Aaron Yip, Michael Weldon, Ida Putu Wiweka Dharmasiddhi, Brian Zubrzycki, Christian Euler, Elisabeth Prince, Yilan Liu, Brian Ingalls, Marc Aucoin

引用次数: 0

Through the looking glass: approaches to visualizing the life cycles of Bdellovibrio and like organisms. 透过镜子：可视化蛭弧菌和类似生物的生命周期的方法。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-07-10 DOI: 10.1139/cjm-2025-0046

Greg Higgins, Ryan M Chanyi, Emma Allen-Vercoe, Cezar M Khursigara, Susan F Koval

引用次数: 0

Extracellular DNA enhances Pseudomonas aeruginosa biofilm resistance to sodium hypochlorite stress. 胞外DNA增强铜绿假单胞菌生物膜对次氯酸钠胁迫的抗性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-07-07 DOI: 10.1139/cjm-2025-0051

Waleska Stephanie Da Cruz Nizer, Madison Elisabeth Adams, Kira Noelle Allison, Carole Beaulieu, Joerg Overhage

{"title":"Extracellular DNA enhances Pseudomonas aeruginosa biofilm resistance to sodium hypochlorite stress.","authors":"Waleska Stephanie Da Cruz Nizer, Madison Elisabeth Adams, Kira Noelle Allison, Carole Beaulieu, Joerg Overhage","doi":"10.1139/cjm-2025-0051","DOIUrl":"https://doi.org/10.1139/cjm-2025-0051","url":null,"abstract":"The opportunistic human pathogen Pseudomonas aeruginosa exhibits high pathogenicity and antimicrobial resistance, largely due to its ability to form robust biofilms. In addition to the exopolysaccharides Psl and Pel, extracellular DNA (eDNA) is an important matrix component in P. aeruginosa PAO1 biofilms. It has been shown previously that eDNA is involved in biofilm initiation and integrity as well as antibiotic resistance, however, it's involvement in resistance to oxidative stressors such as the widely used disinfectant sodium hypochlorite (NaOCl) is less explored. Here, we examined the function of eDNA in NaOCl resistance of P. aeruginosa PAO1 biofilms. Using different biofilm assays in combination with a PAO1 ∆pslA pelF double mutant, which lacks the exopolysaccharides Psl and Pel, and a Tn-bfmR mutant, which exhibits increased eDNA amounts in biofilms, we were able to show that eDNA contributes to NaOCl resistance in P. aeruginosa PAO1 biofilms, in particular when exopolysaccharides are absent. Interestingly, NaOCl was more effective after DNAse treatment against ∆pslA pelF biofilms. These findings indicate that the protective function of eDNA in biofilm resistance is matrix composition-dependent and becomes more pronounced in the absence of Psl and Pel.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-seq analysis of transcription patterns during infection of Mesorhizobium japonicum by phage Cp1R7A-A1. Cp1R7A-A1噬菌体侵染日本中根瘤菌转录模式的RNA-seq分析

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-27 DOI: 10.1139/cjm-2025-0061

Damitha Gunathilake, Kara Dawn Loos, Christopher K Yost, Michael F Hynes

引用次数: 0

Requirements and Considerations for Effective Implementation of Integrated One Health Antimicrobial Resistance Research. 有效实施“一体卫生”抗菌素耐药性综合研究的要求与考虑

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-24 DOI: 10.1139/cjm-2024-0194

Dominic Poulin-Laprade, Jordyn Broadbent, Damien Biot-Pelletier, Susanne Kraemer, Emma J Griffiths, Ayush Kumar, Xian-Zhi Li, Catherine D Carrillo, Rahat Zaheer, Tim A McAllister, Sigrun Kullik, Ernesto Liebana, Nicole Ricker, Alexandra Langlois, Richard Reid-Smith, Sebastien P Faucher, Gabriela Flores-Vargas, Émilie Bédard, J Kimberley Summers, Veronica Jarocki, Thanaporn Thongthum, Carolee A Carson

{"title":"Requirements and Considerations for Effective Implementation of Integrated One Health Antimicrobial Resistance Research.","authors":"Dominic Poulin-Laprade, Jordyn Broadbent, Damien Biot-Pelletier, Susanne Kraemer, Emma J Griffiths, Ayush Kumar, Xian-Zhi Li, Catherine D Carrillo, Rahat Zaheer, Tim A McAllister, Sigrun Kullik, Ernesto Liebana, Nicole Ricker, Alexandra Langlois, Richard Reid-Smith, Sebastien P Faucher, Gabriela Flores-Vargas, Émilie Bédard, J Kimberley Summers, Veronica Jarocki, Thanaporn Thongthum, Carolee A Carson","doi":"10.1139/cjm-2024-0194","DOIUrl":"https://doi.org/10.1139/cjm-2024-0194","url":null,"abstract":"The One Health (OH) approach recognizes the interconnectedness of the health of people, animals, plants/crops and ecosystems, and is central to addressing antimicrobial resistance (AMR). The 7th Environmental Dimension of Antimicrobial Resistance Conference (EDAR7), held in Montreal in May 2024, exemplified this approach by convening international experts and stakeholders to discuss AMR research and policy progress. EDAR7 workshop #8 focused on 1) barriers to establishing effective OH AMR research programs, 2) gaps in OH AMR research priorities, and 3) potential solutions/approaches or 'tools' to ensure programs develop in accordance with OH principles and generate insightful data that maximizes limited resources. Key workshop outcomes included identifying critical principles for OH AMR research programs and highlighting the pivotal role of sustainable data management strategies. Additionally, the importance of considering AMR policy and risk assessment needs when planning and designing research was emphasized. Discussions explored specific tools and approaches that support the standardized and harmonized collection and analysis of data, and associated challenges of integrating genomics data into current risk assessments and models. Synthesis of the workshop's discussions outlined critical considerations that interdisciplinary OH AMR research programs and networks should prioritize to enhance the impact of their outputs.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When DNA Writing is Free: Open Tools and Strategies to Accelerate the Bioeconomy. 当DNA写作是免费的：开放的工具和策略，以加速生物经济。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-18 DOI: 10.1139/cjm-2025-0022

Benjamin Scott, Scott Pownall

{"title":"When DNA Writing is Free: Open Tools and Strategies to Accelerate the Bioeconomy.","authors":"Benjamin Scott, Scott Pownall","doi":"10.1139/cjm-2025-0022","DOIUrl":"https://doi.org/10.1139/cjm-2025-0022","url":null,"abstract":"DNA synthesis and assembly techniques have enabled the creation of validated and standardized DNA parts, used for producing proteins, enzymes, and small molecules. However, most DNA parts are governed by material transfer agreements, which restrict sharing and reuse for commercial purposes even in the absence of patents, bottlenecking innovation. DNA synthesis, crucial for producing new parts, also remains expensive and therefore inaccessible to most researchers. With the breakneck pace of digital innovations for designing and learning from biology, a new and more open approach to the physical building and testing of biology is needed. We propose the establishment of an Open Bio Research Alliance, to create and distribute open collections of DNA and other biological parts, combined with regulated and affordable DNA synthesis services. Focusing on Canada's bioeconomy, establishing domestic DNA synthesis infrastructure would not only secure global competitiveness in engineering biology, but also safeguard biosecurity and national sovereignty over critical resources. By harnessing and supporting existing lab automation resources, the Alliance will also help scale the building and testing of engineered biological systems. Leveraging these tools and strategies, Canada is well-positioned to lead the world in open and innovative biotechnology, paving the way for a thriving bioeconomy.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of aquaculture and genomic surveillance techniques on antimicrobial resistance gene profiles and microbiota detected in marine and freshwater sediments. 水产养殖和基因组监测技术对海洋和淡水沉积物中检测到的抗菌素耐药基因谱和微生物群的影响

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-16 DOI: 10.1139/cjm-2024-0206

Lisa A Johnson, Derek D N Smith, Renuka Subasinghe, Monique Raap, Robin Richter, David Huyben, Calvin Lau, Liam Brown, Jackson Chu, Olena Khomenko, Anthony Manning, Stewart Johnson, Dounia Hamoutene

{"title":"Influence of aquaculture and genomic surveillance techniques on antimicrobial resistance gene profiles and microbiota detected in marine and freshwater sediments.","authors":"Lisa A Johnson, Derek D N Smith, Renuka Subasinghe, Monique Raap, Robin Richter, David Huyben, Calvin Lau, Liam Brown, Jackson Chu, Olena Khomenko, Anthony Manning, Stewart Johnson, Dounia Hamoutene","doi":"10.1139/cjm-2024-0206","DOIUrl":"https://doi.org/10.1139/cjm-2024-0206","url":null,"abstract":"Surveillance methods for antimicrobial resistance genes (ARGs) are needed to assess potential risk of antimicrobial resistance (AMR), especially in complex environmental samples with limited data on ARG distribution. This study employed target-enrichment metagenomics (bait-capture) and a Resistomap qPCR assay to assess the resistome in marine and freshwater sediments associated with active Canadian finfish aquaculture operations. Differences in resistome profiles were considered with distance to the net-pens, concentrations of three aquaculture-associated chemical residues, and microbial communities as detected with 16S rRNA gene amplicon sequencing. With bait-capture, a total of 194 ARGs and 41 replicon types were detected across the sediment samples. Differences due to aquaculture proximity were noted in the composition of the resistome, which was dominated by tetracycline resistance genes. With qPCR, 37 out of 51 ARGs targets were detected, and proximity to net-pens or region did not show changes in resistome composition. Co-occurrence networks revealed significant correlations among genera and the resistome detected with bait-capture, highlighting a potential influence of aquaculture on ARGs in the environment. This study demonstrates the utility of bait-capture and qPCR assays in detection of ARGs in both freshwater and marine sediments from aquaculture sites that will assist further ARG surveillance.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification and community study of root fungal isolates from disturbed and undisturbed arctic and alpine tundra habitats in Northern Quebec, Canada. 加拿大魁北克北部受干扰和未受干扰北极和高山冻土带生境根真菌分离、鉴定和群落研究。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-06-11 DOI: 10.1139/cjm-2024-0211

Léonie Côté, Godar Sene, Marie-Eve Beaulieu, Stéphane Boudreau, Damase P Khasa

{"title":"Isolation, identification and community study of root fungal isolates from disturbed and undisturbed arctic and alpine tundra habitats in Northern Quebec, Canada.","authors":"Léonie Côté, Godar Sene, Marie-Eve Beaulieu, Stéphane Boudreau, Damase P Khasa","doi":"10.1139/cjm-2024-0211","DOIUrl":"https://doi.org/10.1139/cjm-2024-0211","url":null,"abstract":"The use of root symbionts can enhance the establishment and survival of plant communities on drastically disturbed sites. Here, we are interested in root symbionts associated with plants from the Schefferville mining site in northern Quebec. Very few studies of root fungal communities have been conducted in such a northern environment. The spatial and host plant variation of root fungal isolates was investigated. A total of 456 culturable fungi were isolated, of which 376 were successfully identified and assigned to 106 taxa based on rDNA internal transcribed spacer analysis (ITSF-1 and ITS-4). The most frequently isolated fungi belonged to three genetically related groups: the Rhizoscyphusericae aggregate (REA), the dark septate endophytes and Umbelopsisrammaniana and Mortierella sp., which are known to be genetically very close and often misclassified. These groups were present on both disturbed and natural sites, but it appears that plants from disturbed sites had a greater affinity for dark septate endophytes. This study is the first step in the development of a restoration plan for the Schefferville mining site.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of MAPK Signalling and Amino Acid Biosynthesis Underlies the Antifungal Biocontrol Mechanism of Coniochaeta velutina Against Colletotrichum gloeosporioides: A Transcriptome and Metabolome Analysis. MAPK信号传导和氨基酸生物合成的中断是绒毛Coniochaeta对炭疽菌的生物防治机制的基础：转录组和代谢组分析。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-05-21 DOI: 10.1139/cjm-2023-0223

Wei Zheng, Chunling Yang, Chengbo Zhou, Tianxiao Ma, Qingsong Zhu, Zhuoqi Li

{"title":"Disruption of MAPK Signalling and Amino Acid Biosynthesis Underlies the Antifungal Biocontrol Mechanism of Coniochaeta velutina Against Colletotrichum gloeosporioides: A Transcriptome and Metabolome Analysis.","authors":"Wei Zheng, Chunling Yang, Chengbo Zhou, Tianxiao Ma, Qingsong Zhu, Zhuoqi Li","doi":"10.1139/cjm-2023-0223","DOIUrl":"https://doi.org/10.1139/cjm-2023-0223","url":null,"abstract":"This study isolated an endophytic fungus, Coniochaeta velutina, from healthy Camellia oleifera leaves and investigated its inhibitory mechanism on Colletotrichum gloeosporioides using transcriptomics and metabolomics analyses. These helped evaluate the gene expression and metabolite content differences between the treatment and control groups. The transcriptomic analysis detected a total of 15,310 expressed genes, with 3,938 showing significant differential expression (p < 0.05). These genes were primarily enriched in the following pathways: mitogen activated protein kinases (MAPK) signaling, antibiotic biosynthesis, amino acid metabolism, carbon metabolism, and peroxidase pathways. Metabolomics analysis identified 452 metabolites in both groups, with 138 showing significant differences. They were mainly enriched in secondary metabolite biosynthesis, amino acid biosynthesis, and α-linolenic acid metabolism. Transcriptome and metabolome association analysis along with qPCR results significantly increased intracellular pectolinarigenin metabolites in the treatment group, leading to disrupted MAPK signaling and reduced amino acid biosynthesis essential for maintaining normal cell growth. Notably, the decrease in terpenoid compounds was the primary reason for Colletotrichum gloeosporioides being inhibited by the biocontrol fungus Coniochaeta velutina. Therefore, these findings provide valuable insights into the biocontrol mechanisms of Coniochaeta velutina against Colletotrichum gloeosporioides and offer a promising foundation for developing new anthracnose prevention and control strategies.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0