Damitha Gunathilake, Kara Dawn Loos, Christopher K Yost, Michael F Hynes
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RNA-seq analysis of transcription patterns during infection of Mesorhizobium japonicum by phage Cp1R7A-A1.
RNA-Seq analysis of Mesorhizobium japonicum infected by phage Cp1R7A-A1 (full name vB_MloS_Cp1R7A-A1) revealed the transcription of all 237 putative phage genes, including 27 tRNA and 210 protein-coding genes. All predicted protein coding genes of Cp1R7A_A1 showed expression. The phage tRNA genes also showed a notable expression, indicating a possible tRNA-dependant phage survival strategy. Host genes related to nutrient uptake and energy metabolism were significantly upregulated within 1-2 hours post-infection, likely reflecting the phage's metabolic demands. Notably, peptidoglycan-cleaving enzymes showed elevated expression after 3 hours, suggesting host stress responses. These findings show the transcriptional interplay between phage Cp1R7A-A1 and M. japonicum, providing a foundation for future functional studies.
期刊介绍：
Published since 1954, the Canadian Journal of Microbiology is a monthly journal that contains new research in the field of microbiology, including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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