Canadian journal of microbiology最新文献_第10页

Salicylic acids and pathogenic bacteria: new perspectives on an old compound. 水杨酸和致病菌：一种古老化合物的新视角。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-01-01 Epub Date: 2023-09-12 DOI: 10.1139/cjm-2023-0123

Ellen M E Sykes, Dawn White, Sydney McLaughlin, Ayush Kumar

{"title":"Salicylic acids and pathogenic bacteria: new perspectives on an old compound.","authors":"Ellen M E Sykes, Dawn White, Sydney McLaughlin, Ayush Kumar","doi":"10.1139/cjm-2023-0123","DOIUrl":"10.1139/cjm-2023-0123","url":null,"abstract":"Salicylic acids have been used in human and veterinary medicine for their anti-pyretic, anti-inflammatory, and analgesic properties for centuries. A key role of salicylic acid-immune modulation in response to microbial infection-was first recognized during studies of their botanical origin. The effects of salicylic acid on bacterial physiology are diverse. In many cases, they impose selective pressures leading to development of cross-resistance to antimicrobial compounds. Initial characterization of these interactions was in Escherichia coli, where salicylic acid activates the multiple antibiotic resistance (mar) operon, resulting in decreased antibiotic susceptibility. Studies suggest that stimulation of the mar phenotype presents similarly in closely related Enterobacteriaceae. Salicylic acids also affect virulence in many opportunistic pathogens by decreasing their ability to form biofilms and increasing persister cell populations. It is imperative to understand the effects of salicylic acid on bacteria of various origins to illuminate potential links between environmental microbes and their clinically relevant antimicrobial-resistant counterparts. This review provides an update on known effects of salicylic acid and key derivatives on a variety of bacterial pathogens, offers insights to possible potentiation of current treatment options, and highlights cellular regulatory networks that have been established during the study of this important class of medicines.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10571708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild fish from a highly urbanized river (Orge, France) as vectors of culturable Enterobacterales resistant to antibiotics 高度城市化河流（法国奥日河）中的野生鱼类是对抗生素有耐药性的可培养肠杆菌的载体

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-08 DOI: 10.1139/cjm-2023-0121

Anne-Laure Vivant, Etienne Marchand, Benjamin Janvier, T. Berthe, E. Guigon, Nathalie Grall, Fabrice Alliot, Aurélie Goutte, F. Petit

引用次数: 0

Genomic characterization of three bacterial isolates antagonistic to the pea root rot pathogen Aphanomyces euteiches 拮抗豌豆根腐病病原体 Aphanomyces euteiches 的三种细菌分离物的基因组特征

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-07 DOI: 10.1139/cjm-2023-0117

Zakir Hossain, Michelle Hubbard

{"title":"Genomic characterization of three bacterial isolates antagonistic to the pea root rot pathogen Aphanomyces euteiches","authors":"Zakir Hossain, Michelle Hubbard","doi":"10.1139/cjm-2023-0117","DOIUrl":"https://doi.org/10.1139/cjm-2023-0117","url":null,"abstract":"Microorganisms living in soil and rhizosphere or inside plants can promote plant growth and health. Genomic characterization of beneficial microbes could shed light on their special features. Through extensive field survey across Saskatchewan, Canada, followed by in vitro and greenhouse characterization, we identified several bacterial isolates antagonistic to pea root rot pathogen Aphanomyces euteiches. In this study, the genomes of three isolates— Pseudomonas sp. rhizo 66 (PD-S66), Pseudomonas synxantha rhizo 25 (Ps-S25), and Serratia sp. root 2 (TS-R2)—were sequenced, assembled, and annotated. Genome size of PD-S66 was 6 279 416 bp with 65 contigs, 59.32% GC content, and 5653 predicted coding sequences (CDS). Genome size of Ps-S25 was 6 058 437 bp with 66 contigs, a GC content of 60.08%, and 5575 predicted CDS. The genome size of TS-R2 was 5 282 152 bp, containing 26 contigs, a GC content of 56.17%, and 4956 predicted CDS. For the identification of the isolates, digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values were determined, which confirmed PD-S66 and TS-R2 as potential new species, belonging to Pseudomonas and Serratia genera, respectively, while Ps-S25 belongs to species Pseudomonas synxantha. Biosynthetic gene clusters were predicted using antiSMASH. The genomic data provided insight into the genetics and biochemical pathways supporting the antagonistic activity against A. euteiches of these isolates.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"37 24","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of short-term exposure to simulated microgravity on the physiology of Bacillus subtilis and multiomic analysis. 短期模拟微重力环境对枯草芽孢杆菌生理的影响及多组学分析。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-01 Epub Date: 2023-07-18 DOI: 10.1139/cjm-2023-0061

Xiaolei Su, Tingzheng Fang, Lin Fang, Dapeng Wang, Xuege Jiang, Changting Liu, Honglei Zhang, Rui Guo, Junfeng Wang

{"title":"Effects of short-term exposure to simulated microgravity on the physiology of Bacillus subtilis and multiomic analysis.","authors":"Xiaolei Su, Tingzheng Fang, Lin Fang, Dapeng Wang, Xuege Jiang, Changting Liu, Honglei Zhang, Rui Guo, Junfeng Wang","doi":"10.1139/cjm-2023-0061","DOIUrl":"10.1139/cjm-2023-0061","url":null,"abstract":"In our study, Bacillus subtilis was disposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14 days, while the control group was disposed to the same bioreactors in a normal gravity (NG) environment for 14 days. The B. subtilis strain exposed to the SMG (labeled BSS) showed an enhanced growth ability, increased biofilm formation ability, increased sensitivity to ampicillin sulbactam and cefotaxime, and some metabolic alterations compared with the B. subtilis strain under NG conditions (labeled BSN) and the original strain of B. subtilis (labeled BSO). The differentially expressed proteins (DEPs) associated with an increased growth rate, such as DNA strand exchange activity, oxidoreductase activity, proton-transporting ATP synthase complex, and biosynthetic process, were significantly upregulated in BSS. The enhanced biofilm formation ability may be related with the DEPs of spore germination and protein processing in BSS, and differentially expressed genes involved in protein localization and peptide secretion were also significantly enriched. The results revealed that SMG may increase the level of related functional proteins by upregulating or downregulating affiliated genes to change physiological characteristics and modulate growth ability, biofilm formation ability (epsB, epsC, epsN), antibiotic sensitivity (penP) and metabolism. Our experiment may gives new ideas for the study of space microbiology.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"464-478"},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10198931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphatidylinositol-specific phospholipase C can decrease Müller cell viability and suppress its phagocytic activity by modulating PI3K/AKT signaling pathway. 磷脂酰肌醇特异性磷脂酶C可通过调节PI3K/AKT信号通路降低Müller细胞活力并抑制其吞噬活性。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-01 Epub Date: 2023-09-06 DOI: 10.1139/cjm-2023-0044

Bianjin Sun, Shudan Lin, Mengmeng Zheng, Beijia Zheng, Liping Mao, Yunfeng Gu, Jiabei Cai, Yiran Dai, Meiqin Zheng, Yongliang Lou

{"title":"Phosphatidylinositol-specific phospholipase C can decrease Müller cell viability and suppress its phagocytic activity by modulating PI3K/AKT signaling pathway.","authors":"Bianjin Sun, Shudan Lin, Mengmeng Zheng, Beijia Zheng, Liping Mao, Yunfeng Gu, Jiabei Cai, Yiran Dai, Meiqin Zheng, Yongliang Lou","doi":"10.1139/cjm-2023-0044","DOIUrl":"10.1139/cjm-2023-0044","url":null,"abstract":"Bacillus cereus endophthalmitis is a devastating eye infection that causes rapid blindness through the release of extracellular tissue-destructive exotoxins. The phagocytic and antibacterial functions of ocular cells are the keys to limiting ocular bacterial infections. In a previous study, we identified a new virulence gene, plcA-2 (different from the original plcA-1 gene), that was strongly associated with the plcA gene of Listeria monocytogenes. This plcA gene had been confirmed to play an important role in phagocytosis. However, how the Bc-phosphatidylinositol-specific phospholipase C (PI-PLC) proteins encoded by the plcA-1/2 genes affect phagocytes remains unclear in B. cereus endophthalmitis. Here, we found that the enzymatic activity of Bc-PI-PLC-A2 was approximately twofold higher than that of Bc-PI-PLC-A1, and both proteins inhibited the viability of Müller cells. In addition, PI-PLC proteins reduced phagocytosis of Müller cells by decreasing the phosphorylation levels of key proteins in the PI3K/AKT signaling pathway. In conclusion, we showed that PI-PLC proteins contribute to inhibit the viability of and suppress the phagocytosis of Müller cells, providing new insights into the pathogenic mechanism of B. cereus endophthalmitis.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"501-511"},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10225882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virulence-associated genes and antimicrobial resistance patterns in bacteria isolated from pregnant and nonpregnant women with urinary tract infections: the risk of neonatal sepsis. 从患有尿路感染的孕妇和非孕妇身上分离的细菌中的毒力相关基因和抗微生物耐药性模式：新生儿败血症的风险。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-01 Epub Date: 2023-10-10 DOI: 10.1139/cjm-2023-0046

Uriel A Angulo-Zamudio, Hector Flores-Villaseñor, Nidia Leon-Sicairos, Dina Zazueta-Armenta, Francisco A Martínez-Villa, Gabriela Tapia-Pastrana, Jorge Angulo-Rocha, Joel Murillo-Llanes, Mario Francisco Barajas-Olivas, Adrian Canizalez-Roman

{"title":"Virulence-associated genes and antimicrobial resistance patterns in bacteria isolated from pregnant and nonpregnant women with urinary tract infections: the risk of neonatal sepsis.","authors":"Uriel A Angulo-Zamudio, Hector Flores-Villaseñor, Nidia Leon-Sicairos, Dina Zazueta-Armenta, Francisco A Martínez-Villa, Gabriela Tapia-Pastrana, Jorge Angulo-Rocha, Joel Murillo-Llanes, Mario Francisco Barajas-Olivas, Adrian Canizalez-Roman","doi":"10.1139/cjm-2023-0046","DOIUrl":"10.1139/cjm-2023-0046","url":null,"abstract":"Uropathogenic Escherichia coli (UPEC) is classified as the major causative agent of urinary tract infections (UTIs). UPEC virulence and antibiotic resistance can lead to complications in pregnant women and (or) newborns. Therefore, the aim of this study was to determine the etiological agents of UTIs, as well as to identify genes related to virulence factors in bacteria isolated from pregnant and nonpregnant women. A total of 4506 urine samples were collected from pregnant and nonpregnant women. Urine cultures were performed, and PCR was used to identify phylogroups and virulence-related genes. Antibiotic resistance profiles were determined. The incidence of UTIs was 6.9% (pregnant women, n = 206 and nonpregnant women, n = 57), and UPEC belonging to phylogroup A was the most prevalent. The presence of genes related to capsular protection, adhesins, iron acquisition, and serum protection in UPEC was associated with not being pregnant, while the presence of genes related to adhesins was associated with pregnancy. Bacteria isolated from nonpregnant women were more resistant to antibiotics; 36.5% were multidrug resistant, and 34.9% were extensively drug resistant. Finally, UTIs were associated with neonatal sepsis risk, particularly in pregnant women who underwent cesarean section while having a UTI caused by E. coli. In conclusion, UPEC isolated from nonpregnant women carried more virulence factors than those isolated from pregnant women, and maternal UTIs were associated with neonatal sepsis risk.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"488-500"},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of biofilm expansion rate of Bacillus subtilis (MTC871) on agar substrates with different stiffness. 枯草芽孢杆菌(Bacillus subtilis, MTC871)在不同硬度琼脂基质上的生物膜膨胀率分析。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-01 Epub Date: 2023-06-28 DOI: 10.1139/cjm-2022-0259

Jin Wu, Xianyong Li, Rui Kong, Jiankun Wang, Xiaoling Wang

{"title":"Analysis of biofilm expansion rate of Bacillus subtilis (MTC871) on agar substrates with different stiffness.","authors":"Jin Wu, Xianyong Li, Rui Kong, Jiankun Wang, Xiaoling Wang","doi":"10.1139/cjm-2022-0259","DOIUrl":"10.1139/cjm-2022-0259","url":null,"abstract":"The surface morphology of mature biofilms is heterogeneous and can be divided into concentric rings wrinkles (I), labyrinthine networks wrinkles (II), radial ridges wrinkles (III), and branches wrinkles (IV), according to surface wrinkle structure and distribution characteristics. Due to the wrinkle structures, channels are formed between the biofilm and substrate and transport nutrients, water, metabolic products, etc. We find that expansion rate variations of biofilms growing on substrates with high and low agar concentrations (1.5, 2.0, 2.5 wt.%) are not in the same phase. In the first 3 days' growth, the interaction stress between biofilm and each agar substrate increases, which makes the biofilm expansion rate decreases before wrinkle pattern IV (branches) comes up. After 3 days, in the later growth stage after wrinkle pattern IV appears, the biofilm has larger expansion rate growing on 2.0 wt.% agar concentration, which has the larger wrinkle distance in wrinkle pattern IV reducing energy consumption. Our study shows that the stiff substrate does not always inhibit the biofilm expansion, although it does in the earlier stage; after that, mature biofilms acquire larger expansion rate by adjusting the growth mode through the wrinkle evolution even in nutrient extremely depletion.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"479-487"},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10046121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time to treat the climate and nature crisis as one indivisible global health emergency. 是时候将气候和自然危机视为一个不可分割的全球卫生紧急事件。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-12-01 DOI: 10.1139/cjm-2023-0203

引用次数: 0

Role of an antagonistic bacterium, Bacillus subtilis PE7, in growth promotion of netted melon (Cucumis melo L. var. reticulatus Naud.). 拮抗细菌枯草芽孢杆菌PE7在网纹甜瓜生长促进中的作用。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-11-02 DOI: 10.1139/cjm-2023-0083

Seong Eun Han, Jeong-Yong Cho, Kil Yong Kim, Chaw Ei Htwe Maung

{"title":"Role of an antagonistic bacterium, Bacillus subtilis PE7, in growth promotion of netted melon (Cucumis melo L. var. reticulatus Naud.).","authors":"Seong Eun Han, Jeong-Yong Cho, Kil Yong Kim, Chaw Ei Htwe Maung","doi":"10.1139/cjm-2023-0083","DOIUrl":"10.1139/cjm-2023-0083","url":null,"abstract":"The aim of this study was to determine the plant growth-promoting effect of Bacillus subtilis PE7 on growth of melon plants. B. subtilis PE7 isolated from kimchi was identified based on colonial and microscopic morphology along with analyses of 16S rRNA and pycA gene sequences. Strain PE7 showed different levels of inhibition on phytopathogens and was able to grow at variable temperatures and pH values. Strain PE7 had the ability to produce siderophores, indole-3-acetic acid (IAA), ammonia, exopolysaccharides, and 1-aminocyclopropane-1-carboxylic acid deaminase, as well as solubilize insoluble phosphate and zinc. The IAA secretion of strain PE7 showed a concentration-dependent pattern based on the concentration of l-tryptophan supplemented in the fertilizer-based culture medium. The LC-MS analysis indicates the presence of IAA in the culture filtrate of strain PE7. Treatment of the B. subtilis PE7 culture containing different metabolites, mainly IAA, significantly promoted melon growth in terms of higher growth parameters and greater plant nutrient contents compared to treatments with the culture without IAA, fertilizer, and water. The cells of B. subtilis PE7 attached to and firmly colonized the roots of the bacterized melon plants. Based on our results, B. subtilis PE7 can be utilized as a potential microbial fertilizer to substitute chemical fertilizers in sustainable agriculture.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71420910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on. 更正：揭示Deinococcaceae惊人DNA修复能力的科学革命：40 多年过去了。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-11-01 Epub Date: 2023-09-20 DOI: 10.1139/cjm-2023-0128

Michael J Daly

引用次数: 0