Canadian journal of microbiology最新文献_第10页

Lactoferrin modulates the biofilm formation and bap gene expression of methicillin-resistant Staphylococcus epidermidis. 乳铁蛋白调控耐甲氧西林表皮葡萄球菌生物膜形成和bap基因表达。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-02-01 DOI: 10.1139/cjm-2022-0135

Ramona Khanum, Pooi Yin Chung, Stuart C Clarke, Beek Yoke Chin

{"title":"Lactoferrin modulates the biofilm formation and bap gene expression of methicillin-resistant Staphylococcus epidermidis.","authors":"Ramona Khanum, Pooi Yin Chung, Stuart C Clarke, Beek Yoke Chin","doi":"10.1139/cjm-2022-0135","DOIUrl":"https://doi.org/10.1139/cjm-2022-0135","url":null,"abstract":"Lactoferrin is an innate glycoprotein with broad antibacterial and antibiofilm properties. The autonomous antibiofilm activity of lactoferrin against Gram-positive bacteria is postulated to involve the cell wall and biofilm components. Thus, the prevention of biomass formation and eradication of preformed biofilms by lactoferrin was investigated using a methicillin-resistant Staphylococcus epidermidis (MRSE) strain. Additionally, the ability of lactoferrin to modulate the expression of the biofilm-associated protein gene (bap) was studied. The bap gene regulates the production of biofilm-associated proteins responsible for bacterial adhesion and aggregation. In the in vitro biofilm assays, lactoferrin prevented biofilm formation and eradicated established biofilms for up to 24 and 72 h, respectively. Extensive eradication of MRSE biofilm biomass was accompanied by the significant upregulation of bap gene expression. These data suggest the interaction of lactoferrin with the biofilm components and cell wall of MRSE, including the biofilm-associated protein.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10653345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Neighbours in nodules: the interactions between Frankia sp. ACN10a and non-Frankia nodular endophytes of alder. 根瘤中的邻居:桤木根瘤内生菌Frankia sp. ACN10a与非Frankia根瘤内生菌的相互作用。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-02-01 DOI: 10.1139/cjm-2022-0074

Louis Garneau, Pascale B Beauregard, Sébastien Roy

{"title":"Neighbours in nodules: the interactions between Frankia sp. ACN10a and non-Frankia nodular endophytes of alder.","authors":"Louis Garneau, Pascale B Beauregard, Sébastien Roy","doi":"10.1139/cjm-2022-0074","DOIUrl":"https://doi.org/10.1139/cjm-2022-0074","url":null,"abstract":"In the present study, we report the in vitro interactions between Frankia sp. ACN10a and non-Frankia nodular endophytes (NFNE) isolated from alder. The supernatant of NFNE grown in nitrogen-replete medium had neutral or negative effects on Frankia growth; none had a stimulatory effect. Inhibitory effects were observed for supernatants of some NFNE, notably Micromonospora, Pseudomonas, Serratia and Stenotrophomonas isolates. However, some NFNE-Frankia coculture supernatants could stimulate Frankia growth when used as a culture medium supplement. This was observed for supernatants of Frankia cocultured with Microvirga and Streptomyces isolates. In nitrogen-limited conditions, cocultures of Frankia with some NFNE, including some rhizobia and Cytobacillus, resulted in higher total biomass than Frankia-only cultures, suggesting cooperation, while other NFNE were strongly antagonistic. Microscopic observation of cocultures also revealed compromised Frankia membrane integrity, and some differentiation into stress resistance-associated morphotypes such as sporangia and reproductive torulose hyphae (RTH). Furthermore, the coculture of Frankia with Serratia sp. isolates resulted in higher concentrations of the auxinic plant hormone indole-3-acetic acid and related indolic compounds in the culture supernatant. This study sheds new light on the breadth of microbial interactions that occur amongst bacteria that inhabit the understudied ecological niche of the alder nodule.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10653348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial secondary metabolites: possible mechanism for weed suppression in wheat. 细菌次生代谢物:小麦杂草抑制的可能机制。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-02-01 DOI: 10.1139/cjm-2022-0181

Abubakar Dar, Evans Were, Thomas Hilger, Zahir Ahmad Zahir, Maqshoof Ahmad, Azhar Hussain, Frank Rasche

{"title":"Bacterial secondary metabolites: possible mechanism for weed suppression in wheat.","authors":"Abubakar Dar, Evans Were, Thomas Hilger, Zahir Ahmad Zahir, Maqshoof Ahmad, Azhar Hussain, Frank Rasche","doi":"10.1139/cjm-2022-0181","DOIUrl":"https://doi.org/10.1139/cjm-2022-0181","url":null,"abstract":"Chemical weed control is an effective method, but has proved hazardous for humans, environment, and soil biodiversity. Use of allelopathic bacteria may be more efficient and sustainable weed control measure. The bacterial inoculants have never been studied in context of their interaction with weed root exudates and precursor-dependent production of the natural phytotoxins (cyanide, cytolytic enzymes and auxin) by these strains to understand their weed suppression and wheat growth promotion abilities. Therefore, root exudates of Avena fatua, Phalaris minor, Rumex dentatus, and wheat were quantified and their role in microbial root colonization and secondary metabolite production, i.e., cyanide, cytolytic enzymes, phenolics, and elevated auxin concentration, was studied. The results depicted l-tryptophan and glycine as major contributors of elevated cyanide and elevated levels in weed rhizosphere by the studied Pseudomonas strains, through their higher root colonization ability in weeds as compared with wheat. Furthermore, the higher root colonization also enhanced p-coumaric acid (photosynthesis inhibitor by impairing cytochrome c oxidase activity in plants) and cytolytic enzyme (root cell wall degradation) concentration in weed rhizosphere. In conclusion, the differential root colonization of wheat and weeds by these strains is responsible for enhancing weed suppression (enhancing phytotoxic effect) and wheat growth promotion (lowering phytotoxic effect).","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10604502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Deciphering the role of non-Frankia nodular endophytes in alder through in vitro and genomic characterization. 通过体外和基因组表征解读桤木中非法兰克结核内生菌的作用。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-02-01 DOI: 10.1139/cjm-2022-0073

Louis Garneau, Pascale B Beauregard, Sébastien Roy

{"title":"Deciphering the role of non-Frankia nodular endophytes in alder through in vitro and genomic characterization.","authors":"Louis Garneau, Pascale B Beauregard, Sébastien Roy","doi":"10.1139/cjm-2022-0073","DOIUrl":"https://doi.org/10.1139/cjm-2022-0073","url":null,"abstract":"Endophytic bacterial populations are well-positioned to provide benefits to their host plants such as nutrient acquisition and plant hormone level manipulation. Actinorhizal plants such as alders are well known for their microbial symbioses that allow them to colonize harsh environments whether natural or anthropized. Although the nitrogen-fixing actinobacterium Frankia sp. is the main endophyte found in alder root nodules, other bacterial genera, whose roles remain poorly defined, inhabit this niche. In this study, we isolated a diverse panel of non-Frankia nodular endophytes (NFNE). Some NFNE were isolated from alders grown from surface-sterilized seeds and maintained in sterile conditions, suggesting these may have been seed-borne. In vitro testing of 24 NFNE revealed some possessed putative plant growth promotion traits. Their genomes were also sequenced to identify genes related to plant growth promotion traits. This study highlights the complexity of the alder nodular microbial community. It paves the way for further understanding of the biology of nodules and could help improve land reclamation practices that involve alders.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9150200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine-derived antimicrobial molecules from the sponges and their associated bacteria. 来自海绵及其相关细菌的海洋来源的抗菌分子。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2022-0147

Heena Devkar, Narsinh L Thakur, Parvinder Kaur

{"title":"Marine-derived antimicrobial molecules from the sponges and their associated bacteria.","authors":"Heena Devkar, Narsinh L Thakur, Parvinder Kaur","doi":"10.1139/cjm-2022-0147","DOIUrl":"https://doi.org/10.1139/cjm-2022-0147","url":null,"abstract":"Antimicrobial resistance (AMR) is one of the leading global health issues that demand urgent attention. Very soon the world will have to bear the consequences of increased drug resistance if new anti-infectives are not pumped into the clinical pipeline in a short period. This presses on the need for novel chemical entities, and the marine environment is one such hotspot to look for. The Ocean harbours a variety of organisms, of which from this aspect, \"Sponges (Phylum Porifera)\" are of particular interest. To tackle the stresses faced due to their sessile and filter-feeding lifestyle, sponges produce various bioactive compounds, which can be tapped for human use. The sponges harbour several microorganisms of different types and in most cases; the microbial symbionts are the actual producers of the bioactive compounds. This review describes the alarming need for the development of new antimicrobials and how marine sponges can contribute to this. Selected antimicrobial compounds from the marine sponges and their associated bacteria have been described. Additionally, measures to tackle the supply problem have been covered, which is the primary obstacle in marine natural product drug discovery.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10498466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast communities before and after spontaneous fermentation of wine grapes: a case study from Nova Scotia. 酿酒葡萄自发发酵前后的酵母群落:来自新斯科舍省的案例研究。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2022-0179

Adele Bunbury-Blanchette, Lihua Fan, Marcia English, Gavin Kernaghan

{"title":"Yeast communities before and after spontaneous fermentation of wine grapes: a case study from Nova Scotia.","authors":"Adele Bunbury-Blanchette, Lihua Fan, Marcia English, Gavin Kernaghan","doi":"10.1139/cjm-2022-0179","DOIUrl":"https://doi.org/10.1139/cjm-2022-0179","url":null,"abstract":"Wine fermentations are generally completed by the domestic yeast Saccharomyces cerevisiae, but many indigenous vineyard yeasts also influence wine flavour and aroma. Despite the flourishing wine industry in Nova Scotia, there has yet to be any systematic evaluation of these yeasts in Atlantic Canada. The yeast communities of pressed L'Acadie blanc grapes sampled from an organic vineyard in the Annapolis Valley in 2018 and 2019 were characterized before and after spontaneous fermentation by both Illumina and PacBio sequencing, to address and compare potential platform biases. Chemical and sensory evaluations were also conducted. Basidiomycete yeasts, including Vishniacozyma carnescens, Filobasidium globisporum, and Curvibasidium cygneicollum, dominated pre-fermentation diversity. Species of Saccharomyces made up ∼0.04% of sequences prior to fermentation, but 85%-100% after fermentation, with some replicates dominated by S. cerevisiae and some by S. uvarum. PacBio sequencing detected high proportions of Hanseniaspora uvarum, while Illumina sequencing did not. A better understanding of Nova Scotia vineyard yeast communities will allow local wine makers to make better use of non-traditional yeasts and spontaneous fermentations to produce high-quality wines unique to the region.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10498465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to teach life sciences students about dual-use research-a view from the field. 如何向生命科学专业的学生讲授军民两用研究——来自实地的观点。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2021-0341

Michèle Gemünden, Svenja Vinke

{"title":"How to teach life sciences students about dual-use research-a view from the field.","authors":"Michèle Gemünden, Svenja Vinke","doi":"10.1139/cjm-2021-0341","DOIUrl":"https://doi.org/10.1139/cjm-2021-0341","url":null,"abstract":"To reduce biological risks, raising awareness for dual-use issues already at the level of university education is essential. Currently, most life sciences education programs do not incorporate biosecurity and dual-use in their regular curricula. Consequently, the responsibility rests with individual lecturers and depends on their initiative to incorporate dual-use topics into teaching activities. Students interested in biosecurity and dual-use topics often only have the option to educate themselves in external or online courses. Here, we provide practical guidance on how to initiate and integrate a dual-use education program within the curriculum and provide a selection of existing teaching materials. In addition, we suggest key learning objectives to guide the planning of dual-use courses. Different course formats like lectures, seminars, or stand-alone events are discussed regarding their advantages, disadvantages, and suitability for conveying the learning objectives to different educational stages and audiences. As a minimum, we recommend the incorporation of dual-use issues into at least one mandatory course. Ideally, students should additionally participate in in-depth seminars, which can be voluntary and offered in cooperation with external organisations.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10851356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Note of appreciation. 表示感谢。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2022-0255

引用次数: 0

Prototaxites reinterpreted as mega-rhizomorphs, facilitating nutrient transport in early terrestrial ecosystems. 原生红豆杉被重新解释为巨型根状生物，促进了早期陆地生态系统的营养运输。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2021-0358

Vivi Vajda, Larissa Cavalcante, Kristoffer Palmgren, Ashley Krüger, Magnus Ivarsson

{"title":"Prototaxites reinterpreted as mega-rhizomorphs, facilitating nutrient transport in early terrestrial ecosystems.","authors":"Vivi Vajda, Larissa Cavalcante, Kristoffer Palmgren, Ashley Krüger, Magnus Ivarsson","doi":"10.1139/cjm-2021-0358","DOIUrl":"https://doi.org/10.1139/cjm-2021-0358","url":null,"abstract":"The enigmatic fossil Prototaxites found in successions ranging from the Middle Ordovician to the Upper Devonian was originally described as having conifer affinity. The current debate, however, suggests that they probably represent gigantic algal-fungal symbioses. Our re-investigation of permineralized Prototaxites specimens from two localities, the Heider quarry in Germany and the Bordeaux quarry in Canada, reveals striking anatomical similarities with modern fungal rhizomorphs Armillaria mellea. We analysed extant fungal rhizomorphs and fossil Prototaxites through light microscopy of their anatomy, Fourier transform infrared spectroscopy, X-ray microscopy, and Raman spectroscopy. Based on these comparisons, we interpret the Prototaxites as fungi. The detailed preservation of cell walls and possible organelles seen in transverse sections of Prototaxites reveal that fossilization initiated while the organism was alive, inhibiting the collapse of delicate cellular structures. Prototaxites has been interpreted to grow vertically by many previous workers. Here we propose an alternative view that Prototaxites represents a complex hyphal aggregation (rhizomorph) that may have grown horizontally similar to modern complex aggregated mycelial growth forms, such as cords and rhizomorphs. Their main function was possibly to redistribute water and nutrition from nutrient-rich to nutrient-poor areas facilitating the expansion for early land plant communities.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10499990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis of a new type VI secretion system in Vibrio parahaemolyticus and its implications for environmental adaptation in shrimp ponds. 副溶血性弧菌新型VI型分泌系统的基因组分析及其对虾池环境适应的意义。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-01-01 DOI: 10.1139/cjm-2022-0096

Wang Rui, Jinzhou Xiao, Qingyao Wang, Wenyu Zhao, Xinyue Liu, Ying Liu, Songzhe Fu

{"title":"Genomic analysis of a new type VI secretion system in Vibrio parahaemolyticus and its implications for environmental adaptation in shrimp ponds.","authors":"Wang Rui, Jinzhou Xiao, Qingyao Wang, Wenyu Zhao, Xinyue Liu, Ying Liu, Songzhe Fu","doi":"10.1139/cjm-2022-0096","DOIUrl":"https://doi.org/10.1139/cjm-2022-0096","url":null,"abstract":"The type VI secretion system (T6SS) in Vibrio spp. is often used to kill heteroclonal neighbors by direct injection of toxic effectors, but its strategies in aquacultural environments receive limited attention. In this study, we conducted genomic analysis for a T6SS-harboring plasmid in V. parahaemolyticus strain VP157. Coculture assays were further conducted to verify its antibacterial function. The results showed that strain VP157 harbored a 132-kb plasmid, pVP157-1, which consists of two fragments: an 87.8-kb fragment identical to plasmid pTJ114-1 and a 44.2-kb T6SS gene cluster with only 4% DNA identity to T6SS1 in the V. parahaemolyticus reference genome. Gene-by-gene analysis of six genes representing core T6SS components suggested that each gene has distinct evolutionary origins. In vitro experimental evolution revealed that pVP157-1 can excise from the VP157 genome with an excision rate of 4%. A coculture assay suggested that strain VP157 had significantly higher antibacterial activity against Bacillus pumilus and V. cholerae than the strain without pVP157-1(VP157∆T6SS). In contrast, a rapid decline was observed for the proportion of VP157∆ T6SS in a mock microbial community, which decreased from 10.7% to 2.1% in 5 days. The results highlighted that the acquisition of T6SS fostered the fitness of V . parahaemolyticus in a complex environment.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10500139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1