Plant seedlings of peas, tomatoes, and cucumbers exude compounds that are needed for growth and chemoattraction of Rhizobium leguminosarum bv. viciae 3841 and Azospirillum brasilense Sp7.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-01 Epub Date: 2024-03-01 DOI:10.1139/cjm-2023-0217

Fatema A Nisha, Janice N A Tagoe, Amanda B Pease, Shelley M Horne, Angel Ugrinov, Barney A Geddes, Birgit M Prüß

{"title":"Plant seedlings of peas, tomatoes, and cucumbers exude compounds that are needed for growth and chemoattraction of Rhizobium leguminosarum bv. viciae 3841 and Azospirillum brasilense Sp7.","authors":"Fatema A Nisha, Janice N A Tagoe, Amanda B Pease, Shelley M Horne, Angel Ugrinov, Barney A Geddes, Birgit M Prüß","doi":"10.1139/cjm-2023-0217","DOIUrl":null,"url":null,"abstract":"This study characterizes seedling exudates of peas, tomatoes, and cucumbers at the level of chemical composition and functionality. A plant experiment confirmed that Rhizobium leguminosarum bv. viciae 3841 enhanced growth of pea shoots, while Azospirillum brasilense Sp7 supported growth of pea, tomato, and cucumber roots. Chemical analysis of exudates after 1 day of seedling incubation in water yielded differences between the exudates of the three plants. Most remarkably, cucumber seedling exudate did not contain detectable sugars. All exudates contained amino acids, nucleobases/nucleosides, and organic acids, among other compounds. Cucumber seedling exudate contained reduced glutathione. Migration on semi solid agar plates containing individual exudate compounds as putative chemoattractants revealed that R. leguminosarum bv. viciae was more selective than A. brasilense, which migrated towards any of the compounds tested. Migration on semi solid agar plates containing 1:1 dilutions of seedling exudate was observed for each of the combinations of bacteria and exudates tested. Likewise, R. leguminosarum bv. viciae and A. brasilense grew on each of the three seedling exudates, though at varying growth rates. We conclude that the seedling exudates of peas, tomatoes, and cucumbers contain everything that is needed for their symbiotic bacteria to migrate and grow on.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1139/cjm-2023-0217","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/1 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

This study characterizes seedling exudates of peas, tomatoes, and cucumbers at the level of chemical composition and functionality. A plant experiment confirmed that Rhizobium leguminosarum bv. viciae 3841 enhanced growth of pea shoots, while Azospirillum brasilense Sp7 supported growth of pea, tomato, and cucumber roots. Chemical analysis of exudates after 1 day of seedling incubation in water yielded differences between the exudates of the three plants. Most remarkably, cucumber seedling exudate did not contain detectable sugars. All exudates contained amino acids, nucleobases/nucleosides, and organic acids, among other compounds. Cucumber seedling exudate contained reduced glutathione. Migration on semi solid agar plates containing individual exudate compounds as putative chemoattractants revealed that R. leguminosarum bv. viciae was more selective than A. brasilense, which migrated towards any of the compounds tested. Migration on semi solid agar plates containing 1:1 dilutions of seedling exudate was observed for each of the combinations of bacteria and exudates tested. Likewise, R. leguminosarum bv. viciae and A. brasilense grew on each of the three seedling exudates, though at varying growth rates. We conclude that the seedling exudates of peas, tomatoes, and cucumbers contain everything that is needed for their symbiotic bacteria to migrate and grow on.

查看原文本刊更多论文

豌豆、西红柿和黄瓜的幼苗渗出的化合物是豆科根瘤菌（Rhizobium leguminosarum bv. viciae 3841）和巴西绿氮菌（Azospirillum brasilense Sp7）生长和趋化所需的。

本研究从化学成分和功能的角度分析了豌豆、番茄和黄瓜幼苗渗出液的特征。一项植物实验证实，豆荚根瘤菌（Rhizobium leguminosarum bv. viciae 3841）能促进豌豆嫩芽的生长，而巴西鹅膏菌（Azospirillum brasilense Sp7）则能促进豌豆、番茄和黄瓜根系的生长。在水中培育幼苗一天后，对渗出物进行化学分析，发现三种植物的渗出物存在差异。最值得注意的是，黄瓜幼苗的渗出液不含有可检测到的糖分。所有渗出物都含有氨基酸、核碱基/核苷和有机酸等化合物。黄瓜幼苗渗出液中含有还原型谷胱甘肽。在含有单个渗出液化合物作为假定趋化诱导剂的半固体琼脂平板上的迁移表明，R. leguminosarum bv. viciae 比 A. brasilense 的选择性更强，后者会向任何一种测试化合物迁移。在含有 1:1 稀释苗木渗出液的半固体琼脂平板上，可以观察到所测试的每种细菌和渗出液组合的迁移情况。同样，R. leguminosarum bv. viciae 和 A. brasilense 也在三种秧苗渗出液中的每一种上生长，但生长速度各不相同。我们的结论是，豌豆、西红柿和黄瓜的幼苗渗出液中含有共生细菌迁移和生长所需的一切物质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.