Canadian journal of microbiology最新文献_第9页

Responses of bacterial and three sub-microeukaryote communities in the water of white shrimp Penaeus vannamei aquaculture ponds in two polyculture models. 两种混养模式下凡纳滨对虾养殖池水中细菌和三种亚微真核生物群落的响应

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2022-0178

Limin Fan, Fajun Li, Xi Chen, Lihong Shen, Yuyao Chu, Liping Qiu, Gengdong Hu, Chao Song, Dandan Li, Shunlong Meng, Jiazhang Chen

{"title":"Responses of bacterial and three sub-microeukaryote communities in the water of white shrimp Penaeus vannamei aquaculture ponds in two polyculture models.","authors":"Limin Fan, Fajun Li, Xi Chen, Lihong Shen, Yuyao Chu, Liping Qiu, Gengdong Hu, Chao Song, Dandan Li, Shunlong Meng, Jiazhang Chen","doi":"10.1139/cjm-2022-0178","DOIUrl":"https://doi.org/10.1139/cjm-2022-0178","url":null,"abstract":"Polyculture operations in freshwater aquaculture ponds can disrupt microbial communities. High-throughput sequencing was used to assess the impact of polyculture operations on bacterial and three sub-microeukaryote communities (fungi, zooplankton, and eukaryotic phytoplankton) in Penaeus vannamei aquaculture ponds containing oriental river prawns and giant freshwater prawns, respectively. The results showed that the bacterial community was less sensitive than the microeukaryote communities to both the polyculture activity and environmental variations. The polyculture of giant freshwater prawns rather than oriental river prawns was the primary factor affecting the beta diversity of the three sub-microeukaryote communities. This may be due to the larger biomass of the polyculture varieties of giant freshwater prawns compared with oriental river prawns. The polyculture activity of giant freshwater prawns with a higher density and that of oriental river prawns with a lower density increased the stochasticity of the community assembly of the three sub-microeukaryote communities. It also affected the topological properties of the microbial communities, including greater correlations between ecosystem elements, and reducing the correlations among zooplanktons. The eukaryotic phytoplankton was the only microbial community that could also be explained by nutrient variation (mainly the total nitrogen). This highlights the potential role of the eukaryotic phytoplankton as a suitable indicator of the effects of nutrient input into ecosystems.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 7","pages":"242-250"},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9736615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A graduate student's tribute to Dr. R.G.E. Murray. 一名研究生向R.G.E.默里博士致敬。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2023-0057

Ronald S Flannagan

引用次数: 0

The formation mechanism of Bacillus subtilis biofilm surface morphology under competitive environment. 竞争环境下枯草芽孢杆菌生物膜表面形态的形成机制。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2023-0014

Xianyong Li, Rui Kong, Jiankun Wang, Jin Wu, Ketai He, Xiaoling Wang

{"title":"The formation mechanism of Bacillus subtilis biofilm surface morphology under competitive environment.","authors":"Xianyong Li, Rui Kong, Jiankun Wang, Jin Wu, Ketai He, Xiaoling Wang","doi":"10.1139/cjm-2023-0014","DOIUrl":"https://doi.org/10.1139/cjm-2023-0014","url":null,"abstract":"Material properties and growth environments affect the surface morphology of biofilms. Taken the biofilm growing in competitive environments as the object, which is compared with the single biofilm, we find that the competitive environment has an impact on the biofilm thickness and wrinkle patterns. Through diffusion-limited growth theoretical model analysis, it shows that the competitive environment is caused by cells competing for nutrition, and the competitive environment reacts on biofilms, which affect the phenotypic differentiation, causing changes in the stiffness of the biofilm. Using the theoretical and finite element simulation, we compare these results of bi-layer and tri-layer film-substrate models with experimental observations, and find that tri-layer film-substrate model is in line with the reality, which means that the layer between the biofilm and substrate plays an import role for wrinkle formation. Based on the above analysis, we further study effects of biofilm stiffness and interlayer thickness on wrinkles under competitive environment.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 7","pages":"251-261"},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10092853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-performance liquid chromatography-tandem mass spectrometry revealed the significantly different metabolic profiles of Auricularia cornea growing on weakly acidic and weakly alkaline substrates. 超高效液相色谱-串联质谱分析显示，生长在弱酸性和弱碱性基质上的黑木耳代谢谱存在显著差异。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2022-0189

Lei Ye, Bo Zhang, Xuezhen Yang, Xiaolin Li, Wei Tan, Xiaoping Zhang

{"title":"Ultra-performance liquid chromatography-tandem mass spectrometry revealed the significantly different metabolic profiles of Auricularia cornea growing on weakly acidic and weakly alkaline substrates.","authors":"Lei Ye, Bo Zhang, Xuezhen Yang, Xiaolin Li, Wei Tan, Xiaoping Zhang","doi":"10.1139/cjm-2022-0189","DOIUrl":"https://doi.org/10.1139/cjm-2022-0189","url":null,"abstract":"Auricularia cornea is a widely prized basidiomycetous mushroom with culinary and medicine value, which is cultivated artificially on a large scale in China. However, little attention has been paid to the differences in metabolic profiles under different pH growth conditions. In the present study, weakly acidic and weakly alkaline artificial substrates were developed and used for the cultivation of A. cornea, and the metabolic profiles of its fruiting bodies were determined using ultra performance liquid chromatography-tandem mass spectrometry. The results show that the weakly alkaline substrate environment promoted mycelial growth, increased body surface area, and improved yield and transformation efficiency, but attenuated metabolite accumulation by A. cornea. A total of 412 different metabolites were identified in negative and positive ion mode, of which 99 had significantly different amounts and covered 51 metabolic pathways. Principal component analysis and orthogonal patrial least squares discriminant analysis showed clear separation between two treatments, indicating different metabolic profiles. The different metabolites mainly included seven chemical categories, including amino acids and derivatives, nucleotides and derivatives, phenolic acids, organic acids, lipids, flavonoids and alkaloids. This study revealed the biological significance of these metabolites, which could be useful for further unexplored compounds and possible biological functions of A. cornea.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 7","pages":"262-278"},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9730767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Patterns of free-living and particle-attached bacteria along environmental gradients in Lake Taihu. 太湖环境梯度上自由生活和颗粒附着细菌的分布规律。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2022-0243

Guijuan Xie, Robbie M Martin, Changli Liu, Li Zhang, Xiangming Tang

{"title":"Patterns of free-living and particle-attached bacteria along environmental gradients in Lake Taihu.","authors":"Guijuan Xie, Robbie M Martin, Changli Liu, Li Zhang, Xiangming Tang","doi":"10.1139/cjm-2022-0243","DOIUrl":"https://doi.org/10.1139/cjm-2022-0243","url":null,"abstract":"To elucidate the effects of environmental heterogeneity on diversity, composition, and degree of overlap between free-living (FL) and particle-attached (PA) bacteria, we sampled large, shallow, eutrophic Lake Taihu, China across gradients spanning riverine inflow, cyanobacterial blooms, and the open limnetic area. Using high-throughput sequencing of the 16S rRNA gene, we show that (i) bacterial communities near riverine inflow had high α-diversity and a high degree of overlap between FL and PA lifestyles, (ii) communities in cyanobacterial blooms have reduced α-diversity within the PA lifestyle, and (iii) communities from the limnetic area had the lowest bacterial α-diversity within the FL lifestyle and a medium degree of overlap between the FL and PA lifestyles. Redundancy analysis showed that the variation of the FL bacterial community was shaped by suspended solids and total phosphorous, while the variation of the PA bacterial community was shaped by suspended solids, dissolved oxygen, and the percentage of organic matter in suspended solids. This study highlights the importance of environmental heterogeneity, riverine input, cyanobacterial blooms, and nutrient status on the spatial distribution patterns of FL and PA bacterial communities in freshwater lakes.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 6","pages":"228-239"},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dr. RGE Murray: scientist, teacher, and mentor. RGE Murray博士:科学家、教师和导师。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2023-0054

Sharon Walmsley

引用次数: 0

Epidemiology of invasive Haemophilus influenzae disease in northwestern Ontario: comparison of invasive and noninvasive H. influenzae clinical isolates. 安大略省西北部侵袭性流感嗜血杆菌病的流行病学:侵袭性和非侵袭性流感嗜血杆菌临床分离株的比较。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2022-0208

Marina Ulanova, R S W Tsang, E B Nix, L Kelly, M Shuel, B Lance

{"title":"Epidemiology of invasive Haemophilus influenzae disease in northwestern Ontario: comparison of invasive and noninvasive H. influenzae clinical isolates.","authors":"Marina Ulanova, R S W Tsang, E B Nix, L Kelly, M Shuel, B Lance","doi":"10.1139/cjm-2022-0208","DOIUrl":"https://doi.org/10.1139/cjm-2022-0208","url":null,"abstract":"In the post-Haemophilus influenzae type b (Hib) vaccine era, invasive H. influenzae type a (Hia) disease emerged in North American Indigenous populations. The role of Hia in noninvasive disease is uncertain; it is unknown whether noninvasive Hia infections are prevalent in populations with a high incidence of invasive disease, and whether invasive and noninvasive Hia isolates have different characteristics. We analyzed all invasive and noninvasive clinical H. influenzae isolates collected in a northwestern Ontario hospital serving 82% Indigenous population over 5.5 years. Serotyping, clonal analysis, and antimicrobial sensitivity testing were conducted on 233 noninvasive and 20 invasive isolates. Among noninvasive isolates, 91% were nontypeable (NTHi) and 3% were Hia; Hia was the most frequent invasive isolate (60%). Incidence rates of invasive H. influenzae disease (12.5/100 000/year) greatly exceeded average provincial data, with the highest found in <6-year-old children (63.9/100 000/year); the proportion of Hia among invasive isolates was seven times larger than in Ontario. No difference in clonal characteristics between invasive and noninvasive Hia isolates was found. Antibiotic resistance was more common among NTHi than among encapsulated isolates, without differences between invasive and noninvasive isolates. Considering the significance of Hia in Indigenous populations, pediatric immunization against Hia will be useful to prevent serious infections in young Indigenous children.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 6","pages":"219-227"},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know? 机会致病菌热带念珠菌的粘附和生物膜形成:我们知道什么?

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2022-0195

Cássia Milena de Souza, Murilo Moreira Dos Santos, Luciana Furlaneto-Maia, Marcia Cristina Furlaneto

{"title":"Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know?","authors":"Cássia Milena de Souza, Murilo Moreira Dos Santos, Luciana Furlaneto-Maia, Marcia Cristina Furlaneto","doi":"10.1139/cjm-2022-0195","DOIUrl":"https://doi.org/10.1139/cjm-2022-0195","url":null,"abstract":"Candida tropicalis is among the most important Candida species in terms of epidemiology, virulence and resistance. Considering the increase in C. tropicalis incidence and high rates of mortality associated with this species, knowledge of its adhesion and biofilm formation abilities is needed. These traits determine the persistence and survival of yeast on different indwelling medical devices and host sites. C. tropicalis is among the most adherent Candida species, and it has been described as a strong biofilm producer. Environmental factors, phenotypic switching and quorum sensing molecules can affect adhesion and biofilm growth. C. tropicalis can form sexual biofilms, which are promoted by mating pheromones. C. tropicalis biofilms are regulated by a wide and complex network of genes and signaling pathways that are currently poorly understood. Morphological studies showed improved biofilm architecture, which was related to the expression of several hypha-specific genes. Based on recent updates, research is still needed to increase our knowledge on the genetic network of adhesion and biofilm formation by C. tropicalis, as well as the protein diversity that mediates interactions with inert materials and biological surfaces. Here, we have reviewed the main aspects related to adhesion and biofilm formation in C. tropicalis and summarized current knowledge on the significance of these virulence factors in this opportunistic species.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 6","pages":"207-218"},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9550365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Robert G.E. Murray: the consummate editor. 罗伯特·g·e·默里:完美的编辑。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-05-01 DOI: 10.1139/cjm-2023-0033

George A O'Toole

引用次数: 0

Role of the nonribosomal peptide synthetase siderophore enzyme (Rfs) of Mucor lusitanicus in controlling the growth of fungal phytopathogens. lusitanicus毛霉非核糖体肽合成酶铁载体酶(Rfs)在控制真菌植物病原体生长中的作用。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-05-01 DOI: 10.1139/cjm-2022-0203

Viridiana Alejandre-Castañeda, J Alberto Patiño-Medina, Juan Gúzman-Pérez, Marco Ivan Valle-Maldonado, Javier Villegas, Cesar R Solorio-Alvarado, León Francisco Ruiz-Herrera, Rafael Ortiz-Alvarado, Karla Macías-Sánchez, Martha Isela Ramaírez-Díaz, Victor Meza-Carmen

{"title":"Role of the nonribosomal peptide synthetase siderophore enzyme (Rfs) of Mucor lusitanicus in controlling the growth of fungal phytopathogens.","authors":"Viridiana Alejandre-Castañeda, J Alberto Patiño-Medina, Juan Gúzman-Pérez, Marco Ivan Valle-Maldonado, Javier Villegas, Cesar R Solorio-Alvarado, León Francisco Ruiz-Herrera, Rafael Ortiz-Alvarado, Karla Macías-Sánchez, Martha Isela Ramaírez-Díaz, Victor Meza-Carmen","doi":"10.1139/cjm-2022-0203","DOIUrl":"https://doi.org/10.1139/cjm-2022-0203","url":null,"abstract":"Dimorphic species of Mucor, which are cosmopolitan fungi belonging to subphylum Mucoromycotina, are metabolically versatile. Some species of Mucor are sources of biotechnological products, such as biodiesel from Mucor circinelloides and expression of heterologous proteins from Mucor lusitanicus. Furthermore, Mucor lusitanicus has been described as a model for understanding mucormycosis infections. However, little is known regarding the relationship between Mucor lusitanicus and other soil inhabitants. In this study, we investigated the potential use of Mucor lusitanicus as a biocontrol agent against fungal phytopathogens, namely Fusarium oxysporum f. sp. lycopersici, Fusarium solani, and Alternaria solani, which destroy economically important crops. Results showed that aerobic cell-free supernatants of the culture broth (SS) from Mucor lusitanicus inhibited the growth of the fungal phytopathogens in culture, soil, and tomato fruits. The SS obtained from a strain of Mucor lusitanicus carrying the deletion of rfs gene, which encodes an enzyme involved in the synthesis of siderophore rhizoferrin, had a decreased inhibitory effect against the growth of the phytopathogens. Contrarily, this inhibitory effect was more evident with the SS from an rfs-overexpressing strain compared to the wild-type. This study provides a framework for the potential biotechnological use of the molecules secreted from Mucor lusitanicus in the biocontrol of fungal phytopathogens.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 5","pages":"185-198"},"PeriodicalIF":2.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0