Canadian journal of microbiology最新文献_第8页

Constraint on boric acid resistance and tolerance evolvability in Candida albicans. 白念珠菌对硼酸抗性和耐受性进化的制约因素

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-31 DOI: 10.1139/cjm-2023-0225

Yana Syvolos, Ola E Salama, Aleeza C Gerstein

{"title":"Constraint on boric acid resistance and tolerance evolvability in Candida albicans.","authors":"Yana Syvolos, Ola E Salama, Aleeza C Gerstein","doi":"10.1139/cjm-2023-0225","DOIUrl":"10.1139/cjm-2023-0225","url":null,"abstract":"Boric acid is a broad-spectrum antimicrobial used to treat vulvovaginal candidiasis when patients relapse on the primary azole drug fluconazole. Candida albicans is the most common cause of vulvovaginal candidiasis, colloquially referred to as a \"vaginal yeast infection\". Little is known about the propensity of C. albicans to develop BA resistance or tolerance (the ability of a subpopulation to grow slowly in high levels of drug). We evolved 96 replicates from eight diverse C. albicans strains to increasing BA concentrations to test the evolvability of BA resistance and tolerance. Replicate growth was individually assessed daily, with replicates passaged when they had reached an optical density consistent with exponential growth. Many replicates went extinct quickly. Although some replicates could grow in much higher levels of BA than the ancestral strains, evolved populations isolated from the highest terminal BA levels (after 11 weeks of passages) surprisingly showed only modest growth improvements and only at low levels of BA. No large increases in resistance or tolerance were observed in the evolved replicates. Overall, our findings illustrate that there may be evolutionary constraints limiting the emergence of BA resistance and tolerance, which could explain why it remains an effective treatment for recurrent yeast infections.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"384-393"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The limit to evolutionary rescue depends on ploidy in yeast exposed to nystatin. 进化拯救的极限取决于暴露于硝酸菌素的酵母的倍性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1139/cjm-2023-0235

Jasmine Ono, Anastasia Kuzmin, Lesley Miller, Sarah P Otto

{"title":"The limit to evolutionary rescue depends on ploidy in yeast exposed to nystatin.","authors":"Jasmine Ono, Anastasia Kuzmin, Lesley Miller, Sarah P Otto","doi":"10.1139/cjm-2023-0235","DOIUrl":"10.1139/cjm-2023-0235","url":null,"abstract":"The number of copies of each chromosome, or ploidy, of an organism is a major genomic factor affecting adaptation. We set out to determine how ploidy can impact the outcome of evolution, as well as the likelihood of evolutionary rescue, using short-term experiments with yeast (Saccharomyces cerevisiae) in a high concentration of the fungicide nystatin. In similar experiments using haploid yeast, the genetic changes underlying evolutionary rescue were highly repeatable, with all rescued lines containing a single mutation in the ergosterol biosynthetic pathway. All of these beneficial mutations were recessive, which led to the expectation that diploids would find alternative genetic routes to adaptation. To test this, we repeated the experiment using both haploid and diploid strains and found that diploid populations did not evolve resistance. Although diploids are able to adapt at the same rate as haploids to a lower, not fully inhibitory, concentration of nystatin, the present study suggests that diploids are limited in their ability to adapt to an inhibitory concentration of nystatin, while haploids may undergo evolutionary rescue. These results demonstrate that ploidy can tip the balance between adaptation and extinction when organisms face an extreme environmental change.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"394-404"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of a broad host range lytic phage vB_SenS_TUMS_E19 against Salmonella enterica and its efficiency evaluation in the liquid egg. 针对肠炎沙门氏菌的宽宿主范围噬菌体 vB_SenS_TUMS_E19 的特征和基因组分析及其在液态蛋中的效率评估。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-04 DOI: 10.1139/cjm-2024-0013

Narges Torkashvand, Haniyeh Kamyab, Ahmad Reza Shahverdi, Mohammad Reza Khoshayand, Mohammad Amir Karimi Tarshizi, Zargham Sepehrizadeh

{"title":"Characterization and genome analysis of a broad host range lytic phage vB_SenS_TUMS_E19 against Salmonella enterica and its efficiency evaluation in the liquid egg.","authors":"Narges Torkashvand, Haniyeh Kamyab, Ahmad Reza Shahverdi, Mohammad Reza Khoshayand, Mohammad Amir Karimi Tarshizi, Zargham Sepehrizadeh","doi":"10.1139/cjm-2024-0013","DOIUrl":"10.1139/cjm-2024-0013","url":null,"abstract":"Salmonella enterica serovars are zoonotic bacterial that cause foodborne enteritis. Due to bacteria's antibiotic resistance, using bacteriophages for biocontrol and treatment is a new therapeutic approach. In this study, we isolated, characterized, and analyzed the genome of vB_SenS_TUMS_E19 (E19), a broad host range Salmonella bacteriophage, and evaluated the influence of E19 on liquid eggs infected with Salmonella enterica serovar Enteritidis. Transmission electron microscopy showed that the isolated bacteriophage had a siphovirus morphotype. E19 showed rapid adsorption (92% in 5 min), a short latent period (18 min), a large burst size (156 PFU per cell), and a broad host range against different Salmonella enterica serovars. Whole-genome sequencing analysis indicated that the isolated phage had a 42 813 bp long genome with 49.8% G + C content. Neither tRNA genes nor those associated with antibiotic resistance, virulence factors, or lysogenic formation were detected in the genome. The efficacy of E19 was evaluated in liquid eggs inoculated with S. Enteritidis at 4 and 25 °C, and results showed that it could effectively eradicate S. Enteritidis in just 30 min and prevented its growth up to 72 h. Our findings indicate that E19 can be an alternative to a preservative to control Salmonella in food samples and help prevent and treat salmonellosis.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"358-369"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive evolution of Clostridium thermocellum ATCC 27405 on alternate carbon sources leads to altered fermentation profiles. 热梭菌 ATCC 27405 在替代碳源上的适应性进化导致了发酵曲线的改变。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-04 DOI: 10.1139/cjm-2024-0004

Steve R Daley, Samantha Kirby, Richard Sparling

{"title":"Adaptive evolution of Clostridium thermocellum ATCC 27405 on alternate carbon sources leads to altered fermentation profiles.","authors":"Steve R Daley, Samantha Kirby, Richard Sparling","doi":"10.1139/cjm-2024-0004","DOIUrl":"10.1139/cjm-2024-0004","url":null,"abstract":"Consolidated bioprocessing candidate, Clostridium thermocellum, is a cellulose hydrolysis specialist, with the ability to ferment the released sugars to produce bioethanol. C. thermocellum is generally studied with model substrates Avicel and cellobiose to understand the metabolic pathway leading to ethanol. In the present study, adaptive laboratory evolution, allowing C. thermocellum DSM 1237 to adapt to growth on glucose, fructose, and sorbitol, with the prospect that some strains will adapt their metabolism to yield more ethanol. Adaptive growth on glucose and sorbitol resulted in an approximately 1 mM and 2 mM increase in ethanol yield per millimolar glucose equivalent, respectively, accompanied by a shift in the production of the other expected fermentation end products. The increase in ethanol yield observed for sorbitol adapted cells was due to the carbon source being more reduced compared to cellobiose. Glucose and cellobiose have similar oxidation states thus the increase in ethanol yield is due to the rerouting of electrons from other reduced metabolic products excluding H2 which did not decrease in yield. There was no increase in ethanol yield observed for fructose adapted cells, but there was an unanticipated elimination of formate production, also observed in sorbitol adapted cells suggesting that fructose has regulatory implications on formate production either at the transcription or protein level.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"370-383"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterococci as a One Health indicator of antimicrobial resistance. 肠球菌是抗菌药耐药性的一种健康指标。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-08-01 Epub Date: 2024-05-02 DOI: 10.1139/cjm-2024-0024

Sani-E-Zehra Zaidi, Rahat Zaheer, Athanasios Zovoilis, Tim A McAllister

{"title":"Enterococci as a One Health indicator of antimicrobial resistance.","authors":"Sani-E-Zehra Zaidi, Rahat Zaheer, Athanasios Zovoilis, Tim A McAllister","doi":"10.1139/cjm-2024-0024","DOIUrl":"10.1139/cjm-2024-0024","url":null,"abstract":"The rapid increase of antimicrobial-resistant bacteria in humans and livestock is concerning. Antimicrobials are essential for the treatment of disease in modern day medicine, and their misuse in humans and food animals has contributed to an increase in the prevalence of antimicrobial-resistant bacteria. Globally, antimicrobial resistance is recognized as a One Health problem affecting humans, animals, and environment. Enterococcal species are Gram-positive bacteria that are widely distributed in nature. Their occurrence, prevalence, and persistence across the One Health continuum make them an ideal candidate to study antimicrobial resistance from a One Health perspective. The objective of this review was to summarize the role of enterococci as an indicator of antimicrobial resistance across One Health sectors. We also briefly address the prevalence of enterococci in human, animal, and environmental settings. In addition, a 16S RNA gene-based phylogenetic tree was constructed to visualize the evolutionary relationship among enterococcal species and whether they segregate based on host environment. We also review the genomic basis of antimicrobial resistance in enterococcal species across the One Health continuum.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"303-335"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying functional multi-host shuttle plasmids to advance synthetic biology applications in Mesorhizobium and Bradyrhizobium. 鉴定功能性多宿主穿梭质粒，推进中生代和子囊菌的合成生物学应用。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-08-01 Epub Date: 2024-04-02 DOI: 10.1139/cjm-2023-0232

Jordyn S Meaney, Aakanx K Panchal, Aiden J Wilcox, George C diCenzo, Bogumil J Karas

{"title":"Identifying functional multi-host shuttle plasmids to advance synthetic biology applications in Mesorhizobium and Bradyrhizobium.","authors":"Jordyn S Meaney, Aakanx K Panchal, Aiden J Wilcox, George C diCenzo, Bogumil J Karas","doi":"10.1139/cjm-2023-0232","DOIUrl":"10.1139/cjm-2023-0232","url":null,"abstract":"Ammonia availability has a crucial role in agriculture as it ensures healthy plant growth and increased crop yields. Since diazotrophs are the only organisms capable of reducing dinitrogen to ammonia, they have great ecological importance and potential to mitigate the environmental and economic costs of synthetic fertilizer use. Rhizobia are especially valuable being that they can engage in nitrogen-fixing symbiotic relationships with legumes, and they demonstrate great diversity and plasticity in genomic and phenotypic traits. However, few rhizobial species have sufficient genetic tractability for synthetic biology applications. This study established a basic genetic toolbox with antibiotic resistance markers, multi-host shuttle plasmids and a streamlined protocol for biparental conjugation with Mesorhizobium and Bradyrhizobium species. We identified two repABC origins of replication from Sinorhizobium meliloti (pSymB) and Rhizobium etli (p42d) that were stable across all three strains of interest. Furthermore, the NZP2235 genome was sequenced and phylogenetic analysis determined its reclassification to Mesorhizobium huakuii. These tools will enable the use of plasmid-based strategies for more advanced genetic engineering projects and ultimately contribute towards the development of more sustainable agriculture practices by means of novel nitrogen-fixing organelles, elite bioinoculants, or symbiotic association with nonlegumes.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"336-347"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-inoculation with novel nodule-inhabiting bacteria reduces the benefits of legume-rhizobium symbiosis. 与新型结核栖息菌共同接种会降低豆科-根瘤菌共生的益处。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-07-01 Epub Date: 2024-03-20 DOI: 10.1139/cjm-2023-0209

James C Kosmopoulos, Rebecca T Batstone-Doyle, Katy D Heath

{"title":"Co-inoculation with novel nodule-inhabiting bacteria reduces the benefits of legume-rhizobium symbiosis.","authors":"James C Kosmopoulos, Rebecca T Batstone-Doyle, Katy D Heath","doi":"10.1139/cjm-2023-0209","DOIUrl":"10.1139/cjm-2023-0209","url":null,"abstract":"The ecologically and economically vital symbiosis between nitrogen-fixing rhizobia and leguminous plants is often thought of as a bi-partite interaction, yet studies increasingly show the prevalence of non-rhizobial endophytes (NREs) that occupy nodules alongside rhizobia. Yet, what impact these NREs have on plant or rhizobium fitness remains unclear. Here, we investigated four NRE strains found to naturally co-occupy nodules of the legume Medicago truncatula alongside Sinorhizobium meliloti in native soils. Our objectives were to (1) examine the direct and indirect effects of NREs on M. truncatula and S. meliloti fitness, and (2) determine whether NREs can re-colonize root and nodule tissues upon reinoculation. We identified one NRE strain (522) as a novel Paenibacillus species, another strain (717A) as a novel Bacillus species, and the other two (702A and 733B) as novel Pseudomonas species. Additionally, we found that two NREs (Bacillus 717A and Pseudomonas 733B) reduced the fitness benefits obtained from symbiosis for both partners, while the other two (522, 702A) had little effect. Lastly, we found that NREs were able to co-infect host tissues alongside S. meliloti. This study demonstrates that variation of NREs present in natural populations must be considered to better understand legume-rhizobium dynamics in soil communities.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"275-288"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140173779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Year-round monitoring of three water sources in Québec, Canada, reveals site-specific differences in conditions for Cryptosporidium and Giardia contamination. 对加拿大魁北克省三个水源地的全年监测显示，隐孢子虫和贾第鞭毛虫污染的条件因地而异。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-07-01 Epub Date: 2024-05-03 DOI: 10.1139/cjm-2023-0207

Marie-Stéphanie Fradette, Sandrinne L Bourque, Manuel J Rodriguez, Steve J Charette

{"title":"Year-round monitoring of three water sources in Québec, Canada, reveals site-specific differences in conditions for Cryptosporidium and Giardia contamination.","authors":"Marie-Stéphanie Fradette, Sandrinne L Bourque, Manuel J Rodriguez, Steve J Charette","doi":"10.1139/cjm-2023-0207","DOIUrl":"10.1139/cjm-2023-0207","url":null,"abstract":"Cryptosporidium and Giardia are protozoan parasites responsible for gastrointestinal illnesses in humans and in animal species. The main way these parasites are transmitted is by ingestion of their (oo)cysts in drinking water. Monitoring (oo)cysts in water sources is beneficial to evaluate the quality of raw water supplying treatment plants. Currently, the only standardized protocol to enumerate these parasites from water samples is United States Environmental Protection Agency (USEPA) Method 1623.1. With this method, we monitored three major water sources in Quebec over a year to assess temporal and geographical variations of these parasite (oo)cysts. These three water sources have independent watersheds despite being in the same region. We found a general pattern for Giardia, with high concentrations of cysts during cold and transition periods, and significantly lower concentrations during the warm period. Cryptosporidium's concentration was more variable throughout the year. Statistical correlations (Pearson's correlation coefficients) were established between the concentration of each parasite and various environmental parameters. The three study sites each showed unique factors correlating with the presence of both protozoa, supporting the idea that each water source must be seen as a unique entity with its own particular characteristics and therefore, must be monitored independently. Although some environmental parameters could be interesting proxies to the parasitic load, no parameter was strongly correlated throughout the whole sampling year and none of the parameters could be used as a single proxy for all three studies sources.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"262-274"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug repositioning identifies histone deacetylase inhibitors that promote innate immunity in non-tuberculous mycobacterial infection. 药物重新定位发现组蛋白去乙酰化酶抑制剂可促进非结核分枝杆菌感染中的先天免疫。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-07-01 Epub Date: 2024-06-10 DOI: 10.1139/cjm-2023-0127

Adrián Rodriguez-Carlos, Anguita Raúl, Yolanda M Jacobo-Delgado, Carmen Judith Serrano, Alan Santos-Mena, Luis Adrian De Jesus-Gonzalez, Ester Boix, Bruno Rivas-Santiago

{"title":"Drug repositioning identifies histone deacetylase inhibitors that promote innate immunity in non-tuberculous mycobacterial infection.","authors":"Adrián Rodriguez-Carlos, Anguita Raúl, Yolanda M Jacobo-Delgado, Carmen Judith Serrano, Alan Santos-Mena, Luis Adrian De Jesus-Gonzalez, Ester Boix, Bruno Rivas-Santiago","doi":"10.1139/cjm-2023-0127","DOIUrl":"10.1139/cjm-2023-0127","url":null,"abstract":"Non-tuberculosis infections in immunocompromised patients represent a cause for concern, given the increased risks of infection, and limited treatments available. Herein, we report that molecules for binding to the catalytic site of histone deacetylase (HDAC) inhibit its activity, thus increasing the innate immune response against environmental mycobacteria. The action of HDAC inhibitors (iHDACs) was explored in a model of type II pneumocytes and macrophages infection by Mycobacterium aurum. The results show that the use of 1,3-diphenylurea increases the expression of the TLR-4 in M. aurum infected MDMs, as well as the production of defb4, IL-1β, IL-12, and IL-6. Moreover, we observed that aminoacetanilide upregulates the expression of TLR-4 together with TLR-9, defb4, CAMP, RNase 6, RNase 7, IL-1β, IL-12, and IL-6 in T2P. Results conclude that the tested iHDACs selectively modulate the expression of cytokines and antimicrobial peptides that are associated with reduction of non-tuberculous mycobacteria infection.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"252-261"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial communities change along the 300 km length of the Grand River for extreme high- and low-flow regimes. 大河沿岸 300 公里长的微生物群落在极端高流量和低流量情况下的变化。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-07-01 Epub Date: 2024-05-15 DOI: 10.1139/cjm-2023-0092

Taylor L Virgin, Prinpida Sonthiphand, Sara Coyotzi, Michael W Hall, Jason J Venkiteswaran, Richard J Elgood, Sherry L Schiff, Josh D Neufeld

{"title":"Microbial communities change along the 300 km length of the Grand River for extreme high- and low-flow regimes.","authors":"Taylor L Virgin, Prinpida Sonthiphand, Sara Coyotzi, Michael W Hall, Jason J Venkiteswaran, Richard J Elgood, Sherry L Schiff, Josh D Neufeld","doi":"10.1139/cjm-2023-0092","DOIUrl":"10.1139/cjm-2023-0092","url":null,"abstract":"The Grand River watershed is the largest catchment in southern Ontario. The river's northern and southern sections are influenced by agriculture, whereas central regions receive wastewater effluent and urban runoff. To characterize in-river microbial communities, as they relate to spatial and environmental factors, we conducted two same-day sampling events along the entire 300 km length of the river, representing contrasting flow seasons (high flow spring melt and low flow end of summer). Through high-throughput sequencing of 16S rRNA genes, we assessed the relationship between river microbiota and spatial and physicochemical variables. Flow season had a greater impact on communities than spatial or diel effects and profiles diverged with distance between sites under both flow conditions, but low-flow profiles exhibited higher beta diversity. High-flow profiles showed greater species richness and increased presence of soil and sediment taxa, which may relate to increased input from terrestrial sources. Total suspended solids, dissolved inorganic carbon, and distance from headwaters significantly explained microbial community variation during the low-flow event, whereas conductivity, sulfate, and nitrite were significant explanatory factors for spring melt. This study establishes a baseline for the Grand River's microbial community, serving as a foundation for modeling the microbiology of anthropogenically impacted freshwater systems affected by lotic processes.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"289-302"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0