Canadian journal of microbiology最新文献_第8页

Characterization of hydrocarbon degraders from Northwest Passage beach sediments and assessment of their ability for bioremediation. 西北通道海滩沉积物中碳氢化合物降解剂的特征及其生物修复能力评估。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-05-01 Epub Date: 2024-02-13 DOI: 10.1139/cjm-2023-0093

Antoine-O Lirette, Ya-Jou Chen, Nastasia J Freyria, Esteban Góngora, Charles W Greer, Lyle G Whyte

{"title":"Characterization of hydrocarbon degraders from Northwest Passage beach sediments and assessment of their ability for bioremediation.","authors":"Antoine-O Lirette, Ya-Jou Chen, Nastasia J Freyria, Esteban Góngora, Charles W Greer, Lyle G Whyte","doi":"10.1139/cjm-2023-0093","DOIUrl":"10.1139/cjm-2023-0093","url":null,"abstract":"Global warming-induced sea ice loss in the Canadian Northwest Passage (NWP) will result in more shipping traffic, increasing the risk of oil spills. Microorganisms inhabiting NWP beach sediments may degrade hydrocarbons, offering a potential bioremediation strategy. In this study, the characterization and genomic analyses of 22 hydrocarbon-biodegradative bacterial isolates revealed that they contained a diverse range of key alkane and aromatic hydrocarbon-degradative genes, as well as cold and salt tolerance genes indicating they are highly adapted to the extreme Arctic environment. Some isolates successfully degraded Ultra Low Sulfur Fuel Oil (ULSFO) at temperatures as low as -5 °C and high salinities (3%-10%). Three isolates were grown in liquid medium containing ULSFO as sole carbon source over 3 months and variation of hydrocarbon concentration was measured at three time points to determine their rate of hydrocarbon biodegradation. Our results demonstrate that two isolates (Rhodococcus sp. R1B_2T and Pseudarthrobacter sp. R2D_1T) possess complete degradation pathways and can grow on alkane and aromatic components of ULSFO under Arctic conditions. Overall, these results demonstrate that diverse hydrocarbon-degrading microorganisms exist in the NWP beach sediments, offering a potential bioremediation strategy in the events of a marine fuel spill reaching the shores of the NWP.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"163-177"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139729058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial arrangement and density variations in the cell envelope of Deinococcus radiodurans. 放射虫细胞包膜的空间排列和密度变化。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-05-01 Epub Date: 2024-03-25 DOI: 10.1139/cjm-2023-0163

Domenica Farci, Dario Piano

引用次数: 0

Inhibition of the type IV secretion system from antibiotic-resistant Helicobacter pylori clinical isolates supports the potential of Cagα as an anti-virulence target. 抑制抗生素耐药幽门螺旋杆菌临床分离株的 IV 型分泌系统支持了 Cagα 作为抗病毒靶点的潜力。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-04 DOI: 10.1139/cjm-2023-0168

Flore Oudouhou, Claire Morin, Mickael Bouin, Christiane Gaudreau, Christian Baron

{"title":"Inhibition of the type IV secretion system from antibiotic-resistant Helicobacter pylori clinical isolates supports the potential of Cagα as an anti-virulence target.","authors":"Flore Oudouhou, Claire Morin, Mickael Bouin, Christiane Gaudreau, Christian Baron","doi":"10.1139/cjm-2023-0168","DOIUrl":"10.1139/cjm-2023-0168","url":null,"abstract":"Helicobacter pylori resistance to antibiotics is a growing problem and it increasingly leads to treatment failure. While the bacterium is present worldwide, the severity of clinical outcomes is highly dependent on the geographical origin and genetic characteristics of the strains. One of the major virulence factors identified in H. pylori is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) used to translocate effectors into human cells. Here, we investigated the genetic variability of the cagPAI among 13 antibiotic-resistant H. pylori strains that were isolated from patient biopsies in Québec. Seven of the clinical strains carried the cagPAI, but only four could be readily cultivated under laboratory conditions. We observed variability of the sequences of CagA and CagL proteins that are encoded by the cagPAI. All clinical isolates induce interleukin-8 secretion and morphological changes upon co-incubation with gastric cancer cells and two of them produce extracellular T4SS pili. Finally, we demonstrate that molecule 1G2, a small molecule inhibitor of the Cagα protein from the model strain H. pylori 26695, reduces interleukin-8 secretion in one of the clinical isolates. Co-incubation with 1G2 also inhibits the assembly of T4SS pili, suggesting a mechanism for its action on T4SS function.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"119-127"},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the fungal color palette: pigment analysis of Fusarium solani species complex and Curvularia verruculosa clinical isolates. 揭开真菌调色板的神秘面纱：Fusarium solani 种复合菌和 Curvularia verruculosa 临床分离菌的色素分析。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-17 DOI: 10.1139/cjm-2023-0181

Luis Enrique Chaves-González, Daniela Jaikel-Víquez, Stefany Lozada-Alvarado, Fabio Granados-Chinchilla

{"title":"Unveiling the fungal color palette: pigment analysis of Fusarium solani species complex and Curvularia verruculosa clinical isolates.","authors":"Luis Enrique Chaves-González, Daniela Jaikel-Víquez, Stefany Lozada-Alvarado, Fabio Granados-Chinchilla","doi":"10.1139/cjm-2023-0181","DOIUrl":"10.1139/cjm-2023-0181","url":null,"abstract":"Fungal species in the Nectriaceae, such as Fusarium spp. (Hypocreales: Nectriaceae), are etiologic agents of hyalohyphomycosis capable of producing violaceous or yellowish pigments under certain conditions, while Curvularia spp. (Pleosporales: Pleosporaceae) are agents of phaeohyphomycosis and typically produce melanin in their cell walls. In nectriaceous and pleosporaceous fungi, these pigments are mainly constituted by polyketides (e.g., azaphilones, naphthoquinones, and hydroxyanthraquinones). Considering the importance of pigments synthesized by these genera, this work focused on the selective extraction of pigments produced by eight Fusarium solani species complex and one Curvularia verruculosa isolate recovered from dermatomycosis specimens, their separation, purification, and posterior chemical analysis. The pigments were characterized through spectral and acid-base analysis, and their maximum production time was determined. Moreover, spectral identification of isolates was carried out to approach the taxonomic specificity of pigment production. Herein we describe the isolation and characterization of three acidic pigments, yellowish and pinkish azaphilones (i.e., coaherin A and sclerotiorin), and a purplish xanthone, reported for the first time in the Nectriaceae and Pleosporaceae, which appear to be synthesized in a species-independent manner, in the case of fusaria.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"135-149"},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterococcus faecium inhibits NF-κB/NLRP3/Caspase-1 signaling pathway to antagonize enterotoxigenic Escherichia coli-mediated inflammatory response. 粪肠球菌抑制 NF-κB / NLRP3 / Caspase-1 信号通路，以拮抗肠毒性大肠杆菌介导的炎症反应。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-04-01 Epub Date: 2023-12-22 DOI: 10.1139/cjm-2023-0038

Huibin Zheng, Sicheng Pu, Jiahao Liu, Falong Yang, Dechun Chen

{"title":"Enterococcus faecium inhibits NF-κB/NLRP3/Caspase-1 signaling pathway to antagonize enterotoxigenic Escherichia coli-mediated inflammatory response.","authors":"Huibin Zheng, Sicheng Pu, Jiahao Liu, Falong Yang, Dechun Chen","doi":"10.1139/cjm-2023-0038","DOIUrl":"10.1139/cjm-2023-0038","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) can cause intestinal inflammation and diarrhea in yaks, which has a negative impact on their economic value. In recent years, probiotics have gained increasing attention as a pure, natural, nontoxic, harmless, and residue-free additive. However, the underlying mechanisms by which probiotics safeguard against ETEC are not completely elucidated. This study aimed to investigate the protective effect of Enterococcus faecium (E. faecium) against ETEC infection in mice through oral gavage. Morphological changes were examined through light microscopy. The expressions of inflammatory cytokines (IL-1β, IL-6, TNF-α, IL-10, NF-κB, and NLRP3), tight junction protein (ZO-1, Claudin-1), and pyroptosis (Caspase-1, Caspase-4, and gasdermin D (GSDMD)) were detected using immunohistochemistry and quantitative real-time PCR. The results indicate that ETEC infection triggers the activation of inflammation-related pathways (NF-κB) and NLRP3 inflammasome, leading to the expression of a large number of inflammatory cytokines. Additionally, the activation of NLRP3 leads to the release of GSDMD activation through Caspase-1, ultimately resulting in inflammatory injury and pyroptosis. Feeding mice E. faecium early resulted in an increase in the expression of tight junction protein, a reduction in inflammatory cytokines, and alleviation of inflammatory injury and pyroptosis in intestinal tissues. Our research indicates that E. faecium has the ability to antagonize ETEC and provide protection to the gastrointestinal mucosa in mice.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"109-118"},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138884485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QsvR and OpaR coordinately regulate the transcription of cpsS and cpsR in Vibrio parahaemolyticus. QsvR 和 OpaR 协调调节副溶血性弧菌中 cpsS 和 cpsR 的转录。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-04-01 Epub Date: 2024-02-28 DOI: 10.1139/cjm-2023-0196

Xue Li, Wei Lian, Miaomiao Zhang, Xi Luo, Yiquan Zhang, Renfei Lu

{"title":"QsvR and OpaR coordinately regulate the transcription of cpsS and cpsR in Vibrio parahaemolyticus.","authors":"Xue Li, Wei Lian, Miaomiao Zhang, Xi Luo, Yiquan Zhang, Renfei Lu","doi":"10.1139/cjm-2023-0196","DOIUrl":"10.1139/cjm-2023-0196","url":null,"abstract":"Vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis, has a strong capacity to form biofilms on surfaces, which is strictly regulated by the CpsS-CpsR-CpsQ regulatory cascade. OpaR, a master regulator of quorum sensing, is a global regulator that controls multiple cellular pathways including biofilm formation and virulence. QsvR is an AraC-type regulator that works coordinately with OpaR to control biofilm formation and virulence gene expression of V. parahaemolyticus. QsvR and OpaR activate cpsQ transcription. OpaR also activates cpsR transcription, but lacks the detailed regulatory mechanisms. Furthermore, it is still unknown whether QsvR regulates cpsR transcription, as well as whether QsvR and OpaR regulate cpsS transcription. In this study, the results of quantitative real-time PCR and LacZ fusion assays demonstrated that deletion of qsvR and/or opaR significantly decreased the expression levels of cpsS and cpsR compared to the wild-type strain. However, the results of two-plasmid lacZ reporter and electrophoretic mobility-shift assays showed that both QsvR and OpaR were unable to bind the regulatory DNA regions of cpsS and cpsR. Therefore, transcription of cpsS and cpsR was coordinately and indirectly activated by QsvR and OpaR. This work enriched our knowledge on the regulatory network of biofilm formation in V. parahaemolyticus.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"128-134"},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen sulfide regulates arsenic-induced cell death in yeast cells by modulating the antioxidative system. 硫化氢通过调节抗氧化系统调节砷诱导的酵母细胞死亡。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-03-01 Epub Date: 2023-12-14 DOI: 10.1139/cjm-2023-0068

Lihua Wu, Xia Yao, Haiyan Li, Yanfei Chen

{"title":"Hydrogen sulfide regulates arsenic-induced cell death in yeast cells by modulating the antioxidative system.","authors":"Lihua Wu, Xia Yao, Haiyan Li, Yanfei Chen","doi":"10.1139/cjm-2023-0068","DOIUrl":"10.1139/cjm-2023-0068","url":null,"abstract":"Arsenic (As) is a metal with potentially toxic effects on different organisms. Hydrogen sulfide (H2S) plays a vital role in mitigating heavy metal toxicity by reducing oxidative stress in plants and animals. However, the role of H2S in alleviating arsenic toxicity in yeast cells remains unclear. In this study, the role of NaHS (exogenous physiological H2S) in alleviating As-induced yeast cell death was investigated. Yeast cells in the logarithmic phase were pretreated with 0.05 mmol/L NaHS for 6 h, and then incubated in the YPD medium with or without 1 mmol/L As. After 12 h of treatment, relative survival rate, H2S content, oxidative stress biomarkers, and antioxidant machinery were measured. Our results showed that sodium arsenite-induced yeast cell death and pretreatment with 0.05 mmol/L NaHS significantly alleviated sodium arsenite-induced cell death. Under sodium arsenite conditions, the levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) increased, accompanied by the inhibition of the catalase (CAT) activity and the downregulation of CTT1 expression. However, the activities of the superoxide dismutase (SOD) and glutathion peroxidase (GPX) increased, and the expression of SOD1 and GPX2 was markedly upregulated in the group treated with sodium arsenite. When yeast cells were pretreated with NaHS, the intracellular ROS and MDA levels decreased significantly, and the activities of SOD, CAT, and GPX increased significantly. This was associated with a significant increase in relative survival rate and H2S content compared to the arsenic treatment alone. Our findings indicate that NaHS alleviates sodium arsenite-induced yeast cell death, mainly by enhancing the antioxidant defense system.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"102-108"},"PeriodicalIF":2.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the soil fungal communities of steppe grasslands at varying degradation levels in North China. 华北不同退化程度草原土壤真菌群落的变化。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-03-01 Epub Date: 2023-12-14 DOI: 10.1139/cjm-2023-0105

Qiqi Han, Yuhang Chen, Zichao Li, Zhuo Zhang, Yuao Qin, Zhongkuan Liu, Guixia Liu

{"title":"Changes in the soil fungal communities of steppe grasslands at varying degradation levels in North China.","authors":"Qiqi Han, Yuhang Chen, Zichao Li, Zhuo Zhang, Yuao Qin, Zhongkuan Liu, Guixia Liu","doi":"10.1139/cjm-2023-0105","DOIUrl":"10.1139/cjm-2023-0105","url":null,"abstract":"The grasslands in North China are rich in fungal resources. However, the knowledge of the structure and function of fungal communities and the role of microbial communities in vegetation restoration and succession are limited. Thus, we used an Illumina HiSeq PE250 high-throughput sequencing platform to study the changing characteristics of soil fungal communities in degraded grasslands, which were categorized as non-degraded (ND), lightly degraded, moderately degraded, and severely degraded (SD). Moreover, a correlation analysis between soil physical and chemical properties and fungal communities was completed. The results showed that the number of plant species, vegetation coverage, aboveground biomass, and diversity index decreased significantly with increasing degradation, and there were significant differences in the physical and chemical properties of the soil among the different degraded grasslands. The dominant fungal phyla in the degraded grassland were as follows: Ascomycota, 44.88%-65.03%; Basidiomycota, 12.68%-29.91%; and unclassified, 5.51%-16.91%. The dominant fungi were as follows: Mortierella, 6.50%-11.41%; Chaetomium, 6.71%-11.58%; others, 25.95%-36.14%; and unclassified, 25.56%-53.0%. There were significant differences in the microbial Shannon-Wiener and Chao1 indices between the ND and degraded meadows, and the composition and diversity of the soil fungal community differed significantly as the meadows continued to deteriorate. The results showed that pH was the most critical factor affecting soil microbial and fungal communities in SD grasslands, whereas soil microbial and fungal communities in ND grasslands were mainly affected by water content and other environmental factors.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"70-85"},"PeriodicalIF":2.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism. 一种重要的根瘤菌生物控制真菌毛霉 T4 的基因组和转录组测序揭示了与寄生真菌有关的基因。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI: 10.1139/cjm-2023-0148

Yaping Wang, Jian Wang, Xiaochong Zhu, Wei Wang

{"title":"Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism.","authors":"Yaping Wang, Jian Wang, Xiaochong Zhu, Wei Wang","doi":"10.1139/cjm-2023-0148","DOIUrl":"10.1139/cjm-2023-0148","url":null,"abstract":"Trichoderma harzianum is a well-known biological control strain and a mycoparasite of Rhizoctonia solani. To explore the mechanisms of mycoparasitism, the genome and transcriptome of T. harzianum T4 were both assembled and analyzed in this study. The genome of T. harzianum T4 was assembled into 106 scaffolds, sized 41.25 Mb, and annotated with a total of 8118 predicted genes. We analyzed the transcriptome of T. harzianum T4 against R. solani in a dual culture in three culture periods: before contact (BC), during contact (C), and after contact (AC). Transcriptome sequencing identified 1092, 1222, and 2046 differentially expressed genes (DEGs), respectively. These DEGs, which are involved in pathogen recognition and signal transduction, hydrolase, transporters, antibiosis, and defense-related functional genes, are significantly upregulated in the mycoparasitism process. The results of genome and transcriptome analysis indicated that the mycoparasitism process of T. harzianum T4 was very complex. T. harzianum successfully recognizes and invades host cells and kills plant pathogens by regulating various DEGs at different culture periods. The relative expression levels of the 26 upregulated DEGs were confirmed by RT-qPCR to validate the reliability of the transcriptome data. The results provide insight into the molecular mechanisms underlying T. harzianum T4's mycoparasitic processes, and they provide a potential molecular target for the biological control mechanism of T. harzianum T4.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"86-101"},"PeriodicalIF":2.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Note of appreciation. 感谢信

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-01-01 DOI: 10.1139/cjm-2023-0152

引用次数: 0