Canadian journal of microbiology最新文献_第8页

Distribution of corrosive microbial communities in rust layers of steel immersed in seawater. 海水浸泡钢锈蚀层中腐蚀性微生物群落的分布。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-08-01 Epub Date: 2023-05-08 DOI: 10.1139/cjm-2023-0015

Qijing Ren, Yimeng Zhang, Jizhou Duan, Wangqiang Li, Xucheng Dong, Yingying Pei, Ruiyong Zhang, Qingjun Zhu

{"title":"Distribution of corrosive microbial communities in rust layers of steel immersed in seawater.","authors":"Qijing Ren, Yimeng Zhang, Jizhou Duan, Wangqiang Li, Xucheng Dong, Yingying Pei, Ruiyong Zhang, Qingjun Zhu","doi":"10.1139/cjm-2023-0015","DOIUrl":"10.1139/cjm-2023-0015","url":null,"abstract":"In this paper, high-throughput sequencing technology was adopted to visualize the microbial communities on the surfaces of two types of carbon steel immersed in Sea Area Ⅰ. The results showed that different microbial communities were formed on different carbon steel surfaces, in which the genus with the highest abundance on the surface of Q235 was Escherichia-Shigella, while anaerobic Desulfovibrio on the surface of 921a was the most abundant, and the dominant genus varied with the depth of the rust layer. In addition, the distribution of sulfate-reducing bacteria (SRB) on the surface of Q235 submerged in Sea Area Ⅱ was compared with the sulfate-reducing bacteria's distribution in Sea Area Ⅰ, using the environmental factors correlation analysis. The results showed that the concentrations of Ca2+, Na+, K+, Mg2+, and Al3+ were positively correlated with the distribution of SRB, while the concentrations of Cu2+, Zn2+, SO4 2-, Cl-, NO3 -, and organic carbon were negatively correlated with it. Furthermore, there was a highly significant correlation between each geochemical factor and Desulfotomaculum (p < 0.01).","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9891858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An endophytic fungus Schizophyllum commune isolated from Panax ginseng enhances hairy roots growth and ginsenoside biosynthesis. 从人参中分离出一种内生真菌裂褶菌群落，可促进毛状根的生长和人参皂苷的生物合成。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-08-01 Epub Date: 2023-04-21 DOI: 10.1139/cjm-2022-0194

Xing-Guang Xie, Zhen-Zhen Zhang, Ling Chen, Qian-Liang Ming, Ke-Xin Sheng, Xi Chen, Khalid Rahman, Kun-Miao Feng, Juan Su, Ting Han

{"title":"An endophytic fungus Schizophyllum commune isolated from Panax ginseng enhances hairy roots growth and ginsenoside biosynthesis.","authors":"Xing-Guang Xie, Zhen-Zhen Zhang, Ling Chen, Qian-Liang Ming, Ke-Xin Sheng, Xi Chen, Khalid Rahman, Kun-Miao Feng, Juan Su, Ting Han","doi":"10.1139/cjm-2022-0194","DOIUrl":"10.1139/cjm-2022-0194","url":null,"abstract":"Using endophytic fungal elicitors to increase the accumulation of valuable secondary metabolites in plant tissue culture is an effective biotechnology strategy. In this study, a collection of 56 strains of endophytic fungi were isolated from different organs of cultivated Panax ginseng, of which seven strains can be symbiotically co-cultured with the hairy roots of P. ginseng. Further experiments observed that strain 3R-2, identified as endophytic fungus Schizophyllum commune, can not only infect hairy roots but also promote the accumulation of specific ginsenosides. This was further verified because S. commune colonization significantly affected the overall metabolic profile of ginseng hairy roots. By comparing the effects of S. commune mycelia and its mycelia extract (EM) on ginsenoside production in P. ginseng hairy roots, the EM was confirmed to be a relatively better stimulus elicitor. Additionally, the introduction of EM elicitor can significantly enhance the expressions of key enzyme genes of pgHMGR, pgSS, pgSE, and pgSD involved in the biosynthetic pathway of ginsenosides, which was deemed the most relevant factor for promoting ginsenosides production during the elicitation period. In conclusion, this study is the first to show that the EM of endophytic fungus S. commune can be considered as an effective endophytic fungal elicitor for increasing the biosynthesis of ginsenosides in hairy root cultures of P. ginseng.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9903188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of bacterioplankton genes in an impaired Great Lakes harbour reveals seasonal metabolic shifts and a previously undetected cyanobacterium. 对五大湖港口受损浮游细菌基因的分析揭示了季节性代谢变化和以前未发现的蓝藻。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-08-01 DOI: 10.1139/cjm-2022-0252

Christine N Palermo, Roberta R Fulthorpe, Rosemary Saati, Steven M Short

{"title":"Analysis of bacterioplankton genes in an impaired Great Lakes harbour reveals seasonal metabolic shifts and a previously undetected cyanobacterium.","authors":"Christine N Palermo, Roberta R Fulthorpe, Rosemary Saati, Steven M Short","doi":"10.1139/cjm-2022-0252","DOIUrl":"https://doi.org/10.1139/cjm-2022-0252","url":null,"abstract":"Hamilton Harbour is an impaired embayment of Lake Ontario that experiences seasonal algal blooms despite decades of remedial efforts. To study the harbour's cyanobacterial and heterotrophic bacterial communities, we extracted and sequenced community DNA from surface water samples collected biweekly from different sites during summer and fall. Assembled contigs were annotated at the phylum level, and Cyanobacteria were further characterized at order and species levels. Actinobacteria were most abundant in early summer, while Cyanobacteria were dominant in mid-summer. Microcystis aeruginosa and Limnoraphis robusta were most abundant throughout the sampling period, expanding the documented diversity of Cyanobacteria in Hamilton Harbour. Functional annotations were performed using the MG-RAST pipeline and SEED database, revealing that genes for photosynthesis, nitrogen metabolism, and aromatic compound metabolism varied in relative abundances over the season, while phosphorus metabolism was consistent, suggesting that these genes remained essential despite fluctuating environmental conditions and community succession. We observed seasonal shifts from anoxygenic to oxygenic phototrophy, and from ammonia assimilation to nitrogen fixation, coupled with decreasing heterotrophic bacteria and increasing Cyanobacteria relative abundances. Our data contribute important insights into bacterial taxa and functional potentials in Hamilton Harbour, revealing seasonal and spatial dynamics that can be used to inform ongoing remediation efforts.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9916504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of bacterial and three sub-microeukaryote communities in the water of white shrimp Penaeus vannamei aquaculture ponds in two polyculture models. 两种混养模式下凡纳滨对虾养殖池水中细菌和三种亚微真核生物群落的响应

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2022-0178

Limin Fan, Fajun Li, Xi Chen, Lihong Shen, Yuyao Chu, Liping Qiu, Gengdong Hu, Chao Song, Dandan Li, Shunlong Meng, Jiazhang Chen

{"title":"Responses of bacterial and three sub-microeukaryote communities in the water of white shrimp Penaeus vannamei aquaculture ponds in two polyculture models.","authors":"Limin Fan, Fajun Li, Xi Chen, Lihong Shen, Yuyao Chu, Liping Qiu, Gengdong Hu, Chao Song, Dandan Li, Shunlong Meng, Jiazhang Chen","doi":"10.1139/cjm-2022-0178","DOIUrl":"https://doi.org/10.1139/cjm-2022-0178","url":null,"abstract":"Polyculture operations in freshwater aquaculture ponds can disrupt microbial communities. High-throughput sequencing was used to assess the impact of polyculture operations on bacterial and three sub-microeukaryote communities (fungi, zooplankton, and eukaryotic phytoplankton) in Penaeus vannamei aquaculture ponds containing oriental river prawns and giant freshwater prawns, respectively. The results showed that the bacterial community was less sensitive than the microeukaryote communities to both the polyculture activity and environmental variations. The polyculture of giant freshwater prawns rather than oriental river prawns was the primary factor affecting the beta diversity of the three sub-microeukaryote communities. This may be due to the larger biomass of the polyculture varieties of giant freshwater prawns compared with oriental river prawns. The polyculture activity of giant freshwater prawns with a higher density and that of oriental river prawns with a lower density increased the stochasticity of the community assembly of the three sub-microeukaryote communities. It also affected the topological properties of the microbial communities, including greater correlations between ecosystem elements, and reducing the correlations among zooplanktons. The eukaryotic phytoplankton was the only microbial community that could also be explained by nutrient variation (mainly the total nitrogen). This highlights the potential role of the eukaryotic phytoplankton as a suitable indicator of the effects of nutrient input into ecosystems.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9736615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A graduate student's tribute to Dr. R.G.E. Murray. 一名研究生向R.G.E.默里博士致敬。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2023-0057

Ronald S Flannagan

引用次数: 0

The formation mechanism of Bacillus subtilis biofilm surface morphology under competitive environment. 竞争环境下枯草芽孢杆菌生物膜表面形态的形成机制。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2023-0014

Xianyong Li, Rui Kong, Jiankun Wang, Jin Wu, Ketai He, Xiaoling Wang

{"title":"The formation mechanism of Bacillus subtilis biofilm surface morphology under competitive environment.","authors":"Xianyong Li, Rui Kong, Jiankun Wang, Jin Wu, Ketai He, Xiaoling Wang","doi":"10.1139/cjm-2023-0014","DOIUrl":"https://doi.org/10.1139/cjm-2023-0014","url":null,"abstract":"Material properties and growth environments affect the surface morphology of biofilms. Taken the biofilm growing in competitive environments as the object, which is compared with the single biofilm, we find that the competitive environment has an impact on the biofilm thickness and wrinkle patterns. Through diffusion-limited growth theoretical model analysis, it shows that the competitive environment is caused by cells competing for nutrition, and the competitive environment reacts on biofilms, which affect the phenotypic differentiation, causing changes in the stiffness of the biofilm. Using the theoretical and finite element simulation, we compare these results of bi-layer and tri-layer film-substrate models with experimental observations, and find that tri-layer film-substrate model is in line with the reality, which means that the layer between the biofilm and substrate plays an import role for wrinkle formation. Based on the above analysis, we further study effects of biofilm stiffness and interlayer thickness on wrinkles under competitive environment.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10092853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-performance liquid chromatography-tandem mass spectrometry revealed the significantly different metabolic profiles of Auricularia cornea growing on weakly acidic and weakly alkaline substrates. 超高效液相色谱-串联质谱分析显示，生长在弱酸性和弱碱性基质上的黑木耳代谢谱存在显著差异。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-07-01 DOI: 10.1139/cjm-2022-0189

Lei Ye, Bo Zhang, Xuezhen Yang, Xiaolin Li, Wei Tan, Xiaoping Zhang

{"title":"Ultra-performance liquid chromatography-tandem mass spectrometry revealed the significantly different metabolic profiles of Auricularia cornea growing on weakly acidic and weakly alkaline substrates.","authors":"Lei Ye, Bo Zhang, Xuezhen Yang, Xiaolin Li, Wei Tan, Xiaoping Zhang","doi":"10.1139/cjm-2022-0189","DOIUrl":"https://doi.org/10.1139/cjm-2022-0189","url":null,"abstract":"Auricularia cornea is a widely prized basidiomycetous mushroom with culinary and medicine value, which is cultivated artificially on a large scale in China. However, little attention has been paid to the differences in metabolic profiles under different pH growth conditions. In the present study, weakly acidic and weakly alkaline artificial substrates were developed and used for the cultivation of A. cornea, and the metabolic profiles of its fruiting bodies were determined using ultra performance liquid chromatography-tandem mass spectrometry. The results show that the weakly alkaline substrate environment promoted mycelial growth, increased body surface area, and improved yield and transformation efficiency, but attenuated metabolite accumulation by A. cornea. A total of 412 different metabolites were identified in negative and positive ion mode, of which 99 had significantly different amounts and covered 51 metabolic pathways. Principal component analysis and orthogonal patrial least squares discriminant analysis showed clear separation between two treatments, indicating different metabolic profiles. The different metabolites mainly included seven chemical categories, including amino acids and derivatives, nucleotides and derivatives, phenolic acids, organic acids, lipids, flavonoids and alkaloids. This study revealed the biological significance of these metabolites, which could be useful for further unexplored compounds and possible biological functions of A. cornea.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9730767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Patterns of free-living and particle-attached bacteria along environmental gradients in Lake Taihu. 太湖环境梯度上自由生活和颗粒附着细菌的分布规律。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2022-0243

Guijuan Xie, Robbie M Martin, Changli Liu, Li Zhang, Xiangming Tang

{"title":"Patterns of free-living and particle-attached bacteria along environmental gradients in Lake Taihu.","authors":"Guijuan Xie, Robbie M Martin, Changli Liu, Li Zhang, Xiangming Tang","doi":"10.1139/cjm-2022-0243","DOIUrl":"https://doi.org/10.1139/cjm-2022-0243","url":null,"abstract":"To elucidate the effects of environmental heterogeneity on diversity, composition, and degree of overlap between free-living (FL) and particle-attached (PA) bacteria, we sampled large, shallow, eutrophic Lake Taihu, China across gradients spanning riverine inflow, cyanobacterial blooms, and the open limnetic area. Using high-throughput sequencing of the 16S rRNA gene, we show that (i) bacterial communities near riverine inflow had high α-diversity and a high degree of overlap between FL and PA lifestyles, (ii) communities in cyanobacterial blooms have reduced α-diversity within the PA lifestyle, and (iii) communities from the limnetic area had the lowest bacterial α-diversity within the FL lifestyle and a medium degree of overlap between the FL and PA lifestyles. Redundancy analysis showed that the variation of the FL bacterial community was shaped by suspended solids and total phosphorous, while the variation of the PA bacterial community was shaped by suspended solids, dissolved oxygen, and the percentage of organic matter in suspended solids. This study highlights the importance of environmental heterogeneity, riverine input, cyanobacterial blooms, and nutrient status on the spatial distribution patterns of FL and PA bacterial communities in freshwater lakes.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dr. RGE Murray: scientist, teacher, and mentor. RGE Murray博士:科学家、教师和导师。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2023-0054

Sharon Walmsley

引用次数: 0

Epidemiology of invasive Haemophilus influenzae disease in northwestern Ontario: comparison of invasive and noninvasive H. influenzae clinical isolates. 安大略省西北部侵袭性流感嗜血杆菌病的流行病学:侵袭性和非侵袭性流感嗜血杆菌临床分离株的比较。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-06-01 DOI: 10.1139/cjm-2022-0208

Marina Ulanova, R S W Tsang, E B Nix, L Kelly, M Shuel, B Lance

{"title":"Epidemiology of invasive Haemophilus influenzae disease in northwestern Ontario: comparison of invasive and noninvasive H. influenzae clinical isolates.","authors":"Marina Ulanova, R S W Tsang, E B Nix, L Kelly, M Shuel, B Lance","doi":"10.1139/cjm-2022-0208","DOIUrl":"https://doi.org/10.1139/cjm-2022-0208","url":null,"abstract":"In the post-Haemophilus influenzae type b (Hib) vaccine era, invasive H. influenzae type a (Hia) disease emerged in North American Indigenous populations. The role of Hia in noninvasive disease is uncertain; it is unknown whether noninvasive Hia infections are prevalent in populations with a high incidence of invasive disease, and whether invasive and noninvasive Hia isolates have different characteristics. We analyzed all invasive and noninvasive clinical H. influenzae isolates collected in a northwestern Ontario hospital serving 82% Indigenous population over 5.5 years. Serotyping, clonal analysis, and antimicrobial sensitivity testing were conducted on 233 noninvasive and 20 invasive isolates. Among noninvasive isolates, 91% were nontypeable (NTHi) and 3% were Hia; Hia was the most frequent invasive isolate (60%). Incidence rates of invasive H. influenzae disease (12.5/100 000/year) greatly exceeded average provincial data, with the highest found in <6-year-old children (63.9/100 000/year); the proportion of Hia among invasive isolates was seven times larger than in Ontario. No difference in clonal characteristics between invasive and noninvasive Hia isolates was found. Antibiotic resistance was more common among NTHi than among encapsulated isolates, without differences between invasive and noninvasive isolates. Considering the significance of Hia in Indigenous populations, pediatric immunization against Hia will be useful to prevent serious infections in young Indigenous children.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3