Canadian journal of microbiology最新文献_第7页

Polymerase chain reaction-based typing methods and protein profiling analysis of Acinetobacter baumannii isolated from environmental and clinical sources from South India. 基于聚合酶链式反应的分型方法和从南印度环境和临床来源分离的鲍曼不动杆菌的蛋白质谱分析。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-11-01 Epub Date: 2023-06-26 DOI: 10.1139/cjm-2023-0010

Srajana Nayak, D'Almeida Aanice, Dsouza Andria, Archana Pai, Biswajit Maiti

{"title":"Polymerase chain reaction-based typing methods and protein profiling analysis of Acinetobacter baumannii isolated from environmental and clinical sources from South India.","authors":"Srajana Nayak, D'Almeida Aanice, Dsouza Andria, Archana Pai, Biswajit Maiti","doi":"10.1139/cjm-2023-0010","DOIUrl":"10.1139/cjm-2023-0010","url":null,"abstract":"Acinetobacter baumannii is an opportunistic pathogen known for causing hospital-acquired infections. The natural habitat includes soil, water, sewage, and drains, but it is also detected in infected individuals' blood, pus, and respiratory pathways. Due to its resilient nature, it is known to be a causative agent for outbreaks. Therefore, it is crucial to understand the genetic similarity between clinical and environmental isolates. The study aimed to find the genetic relationships between clinical and environmental isolates using PCR-based typing methods such as enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR), random amplified polymorphic DNA (RAPD), and repetitive extragenic palindromic sequence-based PCR (Rep-PCR). Additionally, outer membrane protein (OMP) and whole cell protein (WCP) profiles were also used. The PCR-based methods, ERIC-PCR and Rep-PCR, showed decreased genetic similarity between clinical and environmental isolates (66% and 58%, respectively). However, RAPD showed relatively higher genetic similarity (91%). The OMP and WCP profiles showed varied banding patterns between the clinical and environmental isolates in the 29-43 kDa region. The PCR-based methods proved to be a reliable and reproducible technique. The OMP and WCP profiles, though not as discriminatory as the molecular typing methods, could help identify the most and least commonly occurring protein bands and thus help in typing clinical and environmental A. baumannii isolates.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10036546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on. 科学革命揭示了Deinococcaceae惊人的DNA修复能力：40年过去了。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-10-01 Epub Date: 2023-06-02 DOI: 10.1139/cjm-2023-0059

Michael J Daly

{"title":"The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on.","authors":"Michael J Daly","doi":"10.1139/cjm-2023-0059","DOIUrl":"10.1139/cjm-2023-0059","url":null,"abstract":"The family Deinococcaceae exhibits exceptional radiation resistance and possesses all the necessary traits for surviving in radiation-exposed environments. Their survival strategy involves the coupling of metabolic and DNA repair functions, resulting in an extraordinarily efficient homologous repair of DNA double-strand breaks (DSBs) caused by radiation or desiccation. The keys to their survival lie in the hyperaccumulation of manganous (Mn2+)-metabolite antioxidants that protect their DNA repair proteins under extreme oxidative stress and the persistent structural linkage by Holliday junctions of their multiple genome copies per cell that facilitates DSB repair. This coupling of metabolic and DNA repair functions has made polyploid Deinococcus bacteria a useful tool in environmental biotechnology, radiobiology, aging, and planetary protection. The review highlights the groundbreaking contributions of the late Robert G.E. Murray to the field of Deinococcus research and the emergent paradigm-shifting discoveries that revolutionized our understanding of radiation survivability and oxidative stress defense, demonstrating that the proteome, rather than the genome, is the primary target responsible for survivability. These discoveries have led to the commercial development of irradiated vaccines using Deinococcus Mn-peptide antioxidants and have significant implications for various fields.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on the genus Robertmurraya: a bacterial genus honoring Dr. Robert G.E. Murray (with some personal reminiscences). Robertmurraya属的更新：一个纪念Robert G.E.Murray博士的细菌属（带有一些个人回忆）。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-10-01 Epub Date: 2023-08-09 DOI: 10.1139/cjm-2023-0070

Radhey S Gupta

{"title":"Update on the genus Robertmurraya: a bacterial genus honoring Dr. Robert G.E. Murray (with some personal reminiscences).","authors":"Radhey S Gupta","doi":"10.1139/cjm-2023-0070","DOIUrl":"10.1139/cjm-2023-0070","url":null,"abstract":"The genus Robertmurraya was created by my group in 2020 to recognize the contributions of Dr. Robert G.E. Murray to the field of prokaryotic taxonomy. This manuscript updates the information regarding this genus. In addition to the seven Robertmurraya species with validly published names, the work presented here shows that two species with effectively published names, \"Bacillus yapensis\" and \"Bacillus dakarensis\", and an uncharacterized Bacillus sp. Y1 are also affiliated with this genus. Based on these results, reclassification of \"Bacillus yapensis\" as a novel species Robertmurraya yapensis sp. nov. is proposed. It is also suggested that \"Bacillus dakarensis\", for which strains are not available from culture collections, should also be recognized as \"Robertmurraya dakarensis\". This article also reflects on the serendipitous way I came to know Dr. Murray and his extensive interactions with me and strong support for our work for more than 10 years. Dr. Murray also introduced me and our work to his friend and contemporary Dr. Peter Sneath, who like him also contributed extensively to the field of prokaryotic taxonomy. This introduction led to a fruitful collaboration with Dr. Sneath leading to a joint publication describing the use of the Character Compatibility approach to molecular sequence data.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10193436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of an EPS-producing bifidobacterial strain based on integration of phenotypic and complete genome sequencing data. 基于表型和全基因组测序数据整合的产EPS双歧杆菌菌株的特征。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-10-01 Epub Date: 2023-06-23 DOI: 10.1139/cjm-2023-0025

Dianbin Liu, Haohan Huang, Jinzhi Han, Qiong Wu, Yaoyao Xiang, Yan Liu, Yanxia Wei

{"title":"Characterization of an EPS-producing bifidobacterial strain based on integration of phenotypic and complete genome sequencing data.","authors":"Dianbin Liu, Haohan Huang, Jinzhi Han, Qiong Wu, Yaoyao Xiang, Yan Liu, Yanxia Wei","doi":"10.1139/cjm-2023-0025","DOIUrl":"10.1139/cjm-2023-0025","url":null,"abstract":"Bifidobacterium and Lactobacillus are known to be common members of the human intestinal microbiota, which play important roles in maintaining the homeostasis of host gut microenvironment. Several bifidobacterial and lactobacilli strains have been used as probiotics for health benefits. The exopolysaccharides (EPSs) produced by strains from Bifidobacterium and Lactobacillus are considered as beneficial traits mediating these beneficial effects. In this study, 21 strains belonging to Bifidobacterium and Lactobacillus were isolated from healthy infants' stool and were screened for EPS-producing ability. Among these strains, Bifidobacterium longum XZM1 showed the highest EPS productivity, which was further confirmed and characterized. The complete genome of strain XZM1 was sequenced, which revealed the presence of a gene cluster for EPS production. Furthermore, comparative genome analysis was performed among XZM1 and other strains from B. longum species. Following purification, the molecular weight (Mw) of EPS from XZM1 was determined as 4023 Da (Mw) through gel permeation chromatography. Analysis of the EPS hydrolysates revealed that the EPS was composed of mannose, glucose, galactose, arabinose, and fucose. Additionally, the EPS exhibited higher scavenging abilities toward hydroxyl than 1,1-diphenyl-2-picrylhydrazyl free radical. Overall, these results suggest that XZM1 from B. longum species may be a promising probiotic candidate.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10029392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The VxrAB two-component system is important for the polymyxin B-dependent activation of the type VI secretion system in Vibrio cholerae O1 strain A1552. VxrAB双组分系统对于O1霍乱弧菌菌株A152中VI型分泌系统的多粘菌素B依赖性激活是重要的。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-10-01 Epub Date: 2023-06-21 DOI: 10.1139/cjm-2023-0026

Annabelle Mathieu-Denoncourt, Marylise Duperthuy

{"title":"The VxrAB two-component system is important for the polymyxin B-dependent activation of the type VI secretion system in Vibrio cholerae O1 strain A1552.","authors":"Annabelle Mathieu-Denoncourt, Marylise Duperthuy","doi":"10.1139/cjm-2023-0026","DOIUrl":"10.1139/cjm-2023-0026","url":null,"abstract":"The type VI secretion system (T6SS) is used by bacteria for virulence, resistance to grazing, and competition with other bacteria. We previously demonstrated that the role of the T6SS in interbacterial competition and in resistance to grazing is enhanced in Vibrio cholerae in the presence of subinhibitory concentrations of polymyxin B. Here, we performed a global quantitative proteomic analysis and a targeted transcriptomic analysis of the T6SS-known regulators in V. cholerae grown with and without polymyxin B. The proteome of V. cholerae is greatly modified by polymyxin B with more than 39% of the identified cellular proteins displaying a difference in their abundance, including T6SS-related proteins. We identified a regulator whose abundance and expression are increased in the presence of polymyxin B, vxrB, the response regulator of the two-component system VxrAB (VCA0565-66). In vxrAB, vxrA and vxrB deficient mutants, the expression of both hcp copies (VC1415 and VCA0017), although globally reduced, was not modified by polymyxin B. These hcp genes encode an identical protein Hcp, which is the major component of the T6SS syringe. Thus, the upregulation of the T6SS in the presence of polymyxin B appears to be, at least in part, due to the two-component system VxrAB.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9995157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gentamicin resistance to Escherichia coli related to fatty acid metabolism based on transcriptome analysis. 基于转录组分析的庆大霉素对大肠杆菌的耐药性与脂肪酸代谢有关。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-09-01 Epub Date: 2023-05-24 DOI: 10.1139/cjm-2023-0036

Zhiyu Pan, Juan Guo, Yilin Zhong, Lvyuan Fan, Yubin Su

{"title":"Gentamicin resistance to Escherichia coli related to fatty acid metabolism based on transcriptome analysis.","authors":"Zhiyu Pan, Juan Guo, Yilin Zhong, Lvyuan Fan, Yubin Su","doi":"10.1139/cjm-2023-0036","DOIUrl":"10.1139/cjm-2023-0036","url":null,"abstract":"Antibiotic overuse and misuse have promoted the emergence and spread of antibiotic-resistant bacteria. Increasing bacterial resistance to antibiotics is a major healthcare problem, necessitating elucidation of antibiotic resistance mechanisms. In this study, we explored the mechanism of gentamicin resistance by comparing the transcriptomes of antibiotic-sensitive and -resistant Escherichia coli. A total of 410 differentially expressed genes were identified, of which 233 (56.83%) were up-regulated and 177 (43.17%) were down-regulated in the resistant strain compared with the sensitive strain. Gene Ontology (GO) analysis classifies differential gene expression into three main categories: biological processes, cellular components, and molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the up-regulated genes were enriched in eight metabolic pathways, including fatty acid metabolism, which suggests that fatty acid metabolism may be involved in the development of gentamicin resistance in E. coli. This was demonstrated by measuring the acetyl-CoA carboxylase activity, plays a fundamental role in fatty acid metabolism, was increased in gentamicin-resistant E. coli. Treatment of fatty acid synthesis inhibitor, triclosan, promoted gentamicin-mediated killing efficacy to antibiotic-resistant bacteria. We also found that exogenous addition of oleic acid, which involved in fatty acid metabolism, reduced E. coli sensitivity to gentamicin. Overall, our results provide insight into the molecular mechanism of gentamicin resistance development in E. coli.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10497759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into endophytic fungi diversity and their potential correlation with polyphyllin levels of Paris polyphylla var. yunnanensis. 云南多叶桦内生真菌多样性及其与多叶桦素水平相关性的新认识。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-09-01 DOI: 10.1139/cjm-2023-0056

Juan Lan, Ting Liu, Liyun Miao, Ting Pei, Zhe Gan, Aihua Lin, Hong Geng, Peng Zhang

{"title":"New insights into endophytic fungi diversity and their potential correlation with polyphyllin levels of Paris polyphylla var. yunnanensis.","authors":"Juan Lan, Ting Liu, Liyun Miao, Ting Pei, Zhe Gan, Aihua Lin, Hong Geng, Peng Zhang","doi":"10.1139/cjm-2023-0056","DOIUrl":"https://doi.org/10.1139/cjm-2023-0056","url":null,"abstract":"Endophytes confer fitness advantages to host plants. However, the ecological communities of endophytic fungi in the different tissues (rhizomes, stems, and leaves) of Paris polyphylla and the relationship of their endophytic fungi with polyphyllin levels remain unclear. In this study, the community diversity and differences of endophytic fungi in the rhizomes, stems, and leaves of P. polyphylla var. yunnanensis were investigated, and a comprehensively diverse community of endophytic fungi was represented, including 50 genera, 44 families, 30 orders, 12 classes, and 5 phyla. Distributions of endophytic fungi differed greatly across the three tissues, with six genera common to all tissues, and 11, 5, and 4 genera specific to the rhizomes, stems, and leaves, respectively. Seven genera showed a significantly positive correlation to polyphyllin contents, indicating their potential roles in polyphyllin accumulation. This study provides valuable information for further research of the ecological and biological functions of endophytic fungi of P. polyphylla.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential selection and maintenance of manure-originated multi-drug resistant plasmids at sub-clinical concentrations for tetracycline family antibiotics. 粪便源多重耐药质粒亚临床浓度对四环素类抗生素的潜在选择和维持。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-09-01 DOI: 10.1139/cjm-2022-0240

Tam Thanh Tran, Marlena Cole, Emily Tomas, Andrew Scott, Edward Topp

{"title":"Potential selection and maintenance of manure-originated multi-drug resistant plasmids at sub-clinical concentrations for tetracycline family antibiotics.","authors":"Tam Thanh Tran, Marlena Cole, Emily Tomas, Andrew Scott, Edward Topp","doi":"10.1139/cjm-2022-0240","DOIUrl":"https://doi.org/10.1139/cjm-2022-0240","url":null,"abstract":"The goal of this study was to (a) determine the minimum selection concentrations of tetracycline family antibiotics necessary to maintain plasmids carrying tetracycline-resistant genes and (b) correlate these results to environmental hotspot concentrations reported in previous studies. This study used two plasmids (pT295A and pT413A) originating from dairy manure in a surrogate Escherichia coli host CV601. The minimum selection concentrations of antibiotics tested in nutrient-rich medium were determined as follows: 0.1 mg/L for oxytetracycline, 0.45 mg/L for chlortetracycline, and 0.13-0.25 mg/L for tetracycline. Mixing oxytetracycline and chlortetracycline had minimum selection concentration values increased 2-fold compared to those in single antibiotic tests. Minimum selection concentrations found in this study were lower than reported environmental hotspot concentrations, suggesting that tetracycline family antibiotics were likely to be the driver for the selection and maintenance of these plasmids. Relatively high plasmid loss rates (>90%) were observed when culturing a strain carrying a tetracycline-resistant plasmid in antibiotic-free nutrient-rich and nutrient-defined media. Overall, results suggested that these plasmids can be maintained at concentrations environmentally relevant in wastewater treatment plants, sewage, manure, and manured soil; however, they are unstable and easily lost in the absence of antibiotics.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10145159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of 2-methylpentane in fluid fine tailings amended with a mixture of iso-alkanes under sulfate-reducing conditions. 硫酸盐还原条件下异烷烃混合改性流体细尾矿中2-甲基戊烷的生物降解

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-09-01 DOI: 10.1139/cjm-2023-0022

Mohd Faidz Mohamad Shahimin, Tariq Siddique

{"title":"Biodegradation of 2-methylpentane in fluid fine tailings amended with a mixture of iso-alkanes under sulfate-reducing conditions.","authors":"Mohd Faidz Mohamad Shahimin, Tariq Siddique","doi":"10.1139/cjm-2023-0022","DOIUrl":"https://doi.org/10.1139/cjm-2023-0022","url":null,"abstract":"Anaerobic microorganisms in Canada Natural Upgrading Limited (CNUL) fluid fine tailings (FFT) are sustained by residual solvent hydrocarbons. Although FFT are methanogenic in nature, sulfate-reducing microorganisms represent a significant portion of FFT bacterial community. In this study, we examined biodegradation of three iso-alkanes (2-methylbutane, 2-methylpentane, and 3-methylpentane), representing major iso-alkanes in paraffinic solvent, in CNUL FFT under sulfate-reducing conditions. During ∼1100 days of incubation, only 2-methylpentane was degraded partially, whereas 2-methylbutane and 3-methylpentane were not degraded. During active degradation of 2-methylpentane, the bacterial community was dominated by Anaerolineaceae followed by Syntrophaceae, Peptococcaceae, Desulfobacteraceae, and Desulfobulbaceae. The archaeal community was co-dominated by acetoclastic (Methanosaetaceae) and hydrogenotrophic (Methanobacteriaceae) methanogens. This study underlines the limited capability of the microbial community indigenous to CNUL FFT in degrading recalcitrant iso-alkanes under sulfate-reducing conditions.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10145151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into the composition and diversity of endophytic bacteria in cultivated Huperzia serrata. 栽培沙棘石杉内生细菌组成及多样性新认识。

IF 2.8 4区生物学

Canadian journal of microbiology Pub Date : 2023-08-01 DOI: 10.1139/cjm-2022-0171

Wen-Jing Pan, Li-Yun Miao, Shi-Peng Fan, Pin-Wei Lv, Ai-Hua Lin, Hong Geng, Fa-Jun Song, Peng Zhang

{"title":"New insights into the composition and diversity of endophytic bacteria in cultivated Huperzia serrata.","authors":"Wen-Jing Pan, Li-Yun Miao, Shi-Peng Fan, Pin-Wei Lv, Ai-Hua Lin, Hong Geng, Fa-Jun Song, Peng Zhang","doi":"10.1139/cjm-2022-0171","DOIUrl":"https://doi.org/10.1139/cjm-2022-0171","url":null,"abstract":"Endophytic bacteria play crucial roles in the growth and bioactive compound synthesis of host plants. In this study, the composition and diversity of endophytic bacteria in the roots, stems, and leaves from 3-year-old artificially cultivated Huperzia serrata were investigated using Illumina HiSeq sequencing technology. Total effective reads were assigned to 936 operational taxonomic units (OTUs), belonging to 12 phyla and 289 genera. A total of 28, 3, and 2 OTUs were exclusive to the roots, stems, and leaves, respectively. The bacterial richness and diversity in the roots were significantly lower than those in the leaves and stems. The dominant genera with significant distribution differences among these plant tissue samples were Burkholderia-Caballeronia-Paraburkholderia, Sphingomonas, Acidibacter, Bradyrhizobium, Bryobacter, Methylocella, Nocardioides, Acidothermus, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium. Furthermore, the differences in the bacterial communities associated with these plant tissue samples were visualized using principal coordinate analysis and cluster pedigree diagrams. Linear discriminant analysis effect size explained statistically significant differences among the endophytic bacterial microbiota in these plant tissue samples. Overall, this study provides new insights into the diversity and distribution patterns of endophytic bacteria in the different tissues of H. serrata.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9917315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1