Survival of Antimicrobial Resistant Salmonella Heidelberg Inoculated into Microcosms of Fresh Pine Wood Shavings for Broiler Litter.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-22 DOI:10.1139/cjm-2024-0088

Adelumola Oladeinde, Kimberly Cook, Attiq Rehman, Catherine D Carrillo, Reed Woyda, Crystal Wiersma, Zaid Abdo, Jasmine Johnson, Anna Marie Bosch, Michael Rothrock, Moussa Diarra

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Abstract

This study applied genomic characterizations to a cocktail of three Salmonella enterica serovar Heidelberg (S. Heidelberg) strains different antimicrobial resistance (AMR) profile which were inoculated into fresh pine wood shavings (PWS) microcosms. The strains were isolated from feces (SH-AAFC), carcass (SH-ARS) and thigh (SH-FSIS) of broiler chicken. SH-AAFC harbored an antimicrobial resistant gene (ARG) blaCMY-2 on an IncI1 plasmid while SH-FSIS harbored multiple ARGs (floR, cmlA1, tet(A), blaTEM-1B, ant(2'')-Ia, aph(6)-Id, aph(3'')-Ib and sul2) on an IncC plasmid. SH-ARS was pan-susceptible. The die-off of Salmonella was determined at days 0, 1, 7, 14 and 21. Antibiotic susceptibility tests and whole genome sequencing were performed on 77 isolates. At 21 days post-inoculation, Salmonella abundance decreased by 4.4 Log10 CFU/g with the water activity of PWS being correlated with Salmonella survival. SH-AAFC clonal populations survived longer in PWS than SH-FSIS and SH-ARS populations. SH-AAFC clones persisting in litter carried higher copy number of Col plasmids than their ancestors, while some SH-ARS clones acquired a lysogenic bacteriophage from SH-FSIS populations. These results suggests that mobile genetic determinants such as plasmids (which could carry ARGs) and bacteriophage plays roles in the persistence of S. Heidelberg in the PWS used as broiler litter.

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接种到肉鸡粪便新鲜松木刨花微生态系统中的耐抗菌素海德堡沙门氏菌的存活率

本研究对接种到新鲜松木刨花（PWS）微生态系统中的三株海德堡肠炎沙门氏菌（S. Heidelberg）不同抗菌素耐药性（AMR）特征的鸡尾酒菌株进行了基因组特征分析。这些菌株分别从肉鸡的粪便（SH-AAFC）、胴体（SH-ARS）和大腿（SH-FSIS）中分离出来。SH-AAFC在一个IncI1质粒上携带一个抗菌基因（ARG）blaCMY-2，而SH-FSIS在一个IncC质粒上携带多个ARG（floR、cmlA1、tet(A)、blaTEM-1B、ant(2'')-Ia、ahph(6)-Id、ahph(3'')-Ib和sul2）。SH-ARS 具有泛敏感性。沙门氏菌在第 0、1、7、14 和 21 天死亡。对 77 个分离菌株进行了抗生素药敏试验和全基因组测序。接种后 21 天，沙门氏菌的数量减少了 4.4 Log10 CFU/g，PWS 的水活性与沙门氏菌的存活率相关。与 SH-FSIS和SH-ARS相比，SH-AAFC克隆种群在PWS中存活的时间更长。与它们的祖先相比，SH-AAFC克隆种群的Col质粒拷贝数更高，而一些SH-ARS克隆种群则从SH-FSIS种群中获得了溶菌性噬菌体。这些结果表明，质粒（可能携带ARGs）和噬菌体等移动遗传决定因素在海德堡氏菌在用作肉鸡窝料的PWS中的持续存在中发挥了作用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.