Lettuce seed pelleting with Pseudomonas sp. 31-12: plant growth promotion under laboratory and greenhouse conditions.

Abstract

Plant growth promotion by Pseudomonas sp. 31-12 incorporated into a lettuce seed pelleting matrix was studied. We examined (1) the effect of five rhizosphere derived bacterial strains on green oak lettuce (Lactuca sativa L.) seed germination, root and shoot growth, as a strain selection step for seed coating and seed pelletizing studies, (2) population stability of Pseudomonas sp. 31-12 incorporated into a pelleting matrix on lettuce seed stored three months at 4 °C, and (3) lettuce growth promotion in the laboratory and greenhouse by Pseudomonas sp. 31-12 coated and pelletized seed. A spontaneous streptomycin mutant of Pseudomonas sp. 31-12 (str) was used to determine population size on seed and roots of 15- and 30-day-old lettuce. The population of Pseudomonas sp. 31-12str on coated and pelleted seed decreased from 10⁴ cfu/seed to 10³ cfu/seed after 3 months storage at 4 °C. However, the population exceeded 10⁴ cfu/g root dry mass and 10⁵/g root dry mass after 15 days and 30 days in the greenhouse. Leaf fresh mass was significantly increased (P ≤ 0.05) with Pseudomonas sp. 31-12 seed treatment as compared to noninoculated seed. In conclusion, pelletized lettuce seed with Pseudomonas sp. 31-12 promoted growth and yield in the greenhouse.