M13噬菌体作为基因工程创造新材料的多功能平台。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Julia Maria de Medeiros Dantas, Reefah Fahmida Kabir, Daniel Modafferi, Beyza Ozbaran, Noémie-Manuelle Dorval Courchesne
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引用次数: 0

摘要

M13噬菌体形成自组装纳米棒,具有自组装成具有高阶结构的复杂材料的能力。这些特性使它们成为材料制造的有用模板。它们在软材料、生物纳米系统和生物医学应用中的应用已经得到了很好的确立。对于这些生物界面应用,噬菌体保持生物相容性及其生产的可持续性至关重要。在这里,我们回顾了M13噬菌体的生物加工和在纳米材料或块状材料中保留其天然资产的基因工程策略。具体而言,我们重点介绍了广泛研究的M13噬菌体与大肠杆菌(E. coli)的发酵过程以及适用于材料制造的常见下游加工方法。噬菌体的易于生产有助于其广泛用于噬菌体展示,使大型功能突变体文库的创建成为可能。出于材料目的,基因工程通常针对pIII和pVIII蛋白,从而实现不同的几何形状和片段大小。我们还回顾了噬菌体上显示的常见肽,包括RGD,用于SPR探针,靶向药物,细胞再生或组织支架。我们研究了谷氨酸修饰的噬菌体在金属结合、生物矿化和电子材料中的应用。通过考虑自组装、生物加工和基因工程,材料工程师可以充分利用M13噬菌体制造各种功能和可持续的设备。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
M13 bacteriophage as a versatile platform for the creation of new materials via genetic engineering.

M13 bacteriophages form self-assembled nanorods with the ability to self-assemble into complex materials with higher-order structures. These features make them useful templates for material fabrication. Their use in soft materials, bio-nano systems, and biomedical applications is well established. For these bio-interfacial applications, it is crucial that phages remain biocompatible and their production sustainable. Here, we review the bioprocessing of M13 phages and genetic engineering strategies that retain their natural assets in nanomaterials or bulk materials. Specifically, we highlight the extensively studied fermentation process of M13 phages with Escherichia coli (E. coli) and common downstream processing methods suitable for materials manufacturing. The ease of phage production contributes to its wide use for phage display, enabling the creation of large libraries of functional mutants. For materials purposes, genetic engineering often targets the pIII and pVIII proteins, enabling different geometries and fragment sizes. We also review common peptides displayed on phages, including arginine-glycine-aspartic acid (RGD) peptides, used for surface plasmon resonance (SPR) probes, targeted medicine, cell regeneration, or tissue scaffolding. We study glutamate-modified phages for metal binding, biomineralization, and electronics in bulk materials. By considering self-assembly, bioprocessing, and genetic engineering, material engineers can fully harness M13 phages for diverse functional and sustainable devices.

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来源期刊
CiteScore
4.80
自引率
0.00%
发文量
71
审稿时长
2.5 months
期刊介绍: Published since 1954, the Canadian Journal of Microbiology is a monthly journal that contains new research in the field of microbiology, including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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