Canadian journal of microbiology最新文献_第5页

Prairie soil improves wheat establishment and accelerates the developmental transition to flowering compared to agricultural soils. 与农业土壤相比，草原土壤能改善小麦的生长发育，并加快向开花期的过渡。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-11-01 Epub Date: 2024-08-07 DOI: 10.1139/cjm-2023-0237

Renee H Petipas, Cassidy Peru, Janice M Parks, Maren L Friesen, Chandra N Jack

{"title":"Prairie soil improves wheat establishment and accelerates the developmental transition to flowering compared to agricultural soils.","authors":"Renee H Petipas, Cassidy Peru, Janice M Parks, Maren L Friesen, Chandra N Jack","doi":"10.1139/cjm-2023-0237","DOIUrl":"10.1139/cjm-2023-0237","url":null,"abstract":"Less than 1% of native prairie lands remain in the United States. Located in eastern Washington, the rare habitat called Palouse prairie was largely converted to wheat monocropping. With this conversion came numerous physical, chemical, and biological changes to the soil that may ultimately contribute to reduced wheat yields. Here, we explored how wheat (Tritcum aestivum L.) seedling establishment, plant size, and heading, signifying the developmental transition to flowering, were affected by being planted in prairie soil versus agricultural soils. We then sought to understand whether the observed effects were the result of changes to the soil microbiota due to agricultural intensification. We found that prairie soil enhanced both the probability of wheat seedling survival and heading compared to agricultural soil; however, wheat growth was largely unaffected by soil source. We did not detect effects on wheat developmental transitions or phenotype when inoculated with prairie microbes compared with agricultural microbes, but we did observe general antagonistic effects of microbes on plant size, regardless of soil source. This work indicates that agricultural intensification has affected soils in a way that changes early seedling establishment and the timing of heading for wheat, but these effects may not be caused by microbes, and instead may be caused by soil nutrient conditions.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"482-491"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and characterization of plasmid-encoded qnr genes in quinolone-resistant bacteria across diverse aquatic environments in southern Ontario. 安大略省南部不同水生环境中抗喹诺酮细菌质粒编码 qnr 基因的出现和特征。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-11-01 Epub Date: 2024-07-31 DOI: 10.1139/cjm-2024-0029

Farhan Yusuf, Saher M Ahmed, Danica Dy, Katherine Baney, Hassan Waseem, Kimberley A Gilbride

{"title":"Occurrence and characterization of plasmid-encoded qnr genes in quinolone-resistant bacteria across diverse aquatic environments in southern Ontario.","authors":"Farhan Yusuf, Saher M Ahmed, Danica Dy, Katherine Baney, Hassan Waseem, Kimberley A Gilbride","doi":"10.1139/cjm-2024-0029","DOIUrl":"10.1139/cjm-2024-0029","url":null,"abstract":"Antimicrobial resistance is an ever-increasing threat. The widespread usage of ciprofloxacin has led to the manifestation of resistance due to chromosomal mutations or the acquisition of plasmid-mediated quinolone resistance (PMQR) traits. Some particular PMQR traits, qnr genes, have been identified globally in clinical and environmental isolates. This study aimed to determine the prevalence of ciprofloxacin-resistant bacteria in aquatic environments in southern Ontario and investigate the extent of dissemination of ciprofloxacin resistance traits among the bacterial communities. We surveyed the prevalence of plasmid encoding qnr genes using a multiplex PCR assay of associated PMQR genes, qnrA, qnrB, and qnrS, on 202 isolates. Despite the absence of significant impacts on minimum inhibitory concentration levels, the presence of qnr genes correlates with heightened resistance to quinolones and nalidixic acid in some isolates. Taxonomic analysis highlights distinct differences in the composition and diversity of ciprofloxacin-sensitive (CipS) and ciprofloxacin-resistant (CipR) populations, with Proteobacteria dominating both groups. Importantly, CipR populations exhibit lower genetic diversity but higher prevalence of multiple antibiotic resistances, suggesting co-selection mechanisms. Co-occurrence analysis highlights significant associations between ciprofloxacin resistance and other antibiotic resistances, implicating complex genetic linkages. The results of our study signified the critical role of environmental monitoring in public health.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"492-506"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogeochemical stability of organic covers and mine wastes under climate change simulated mesocosms. 气候变化模拟介观模式下有机覆盖物和矿山废物的生物地球化学稳定性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI: 10.1139/cjm-2024-0064

Asma Asemaninejad, Ted Mackinnon, Sean Langley

{"title":"Biogeochemical stability of organic covers and mine wastes under climate change simulated mesocosms.","authors":"Asma Asemaninejad, Ted Mackinnon, Sean Langley","doi":"10.1139/cjm-2024-0064","DOIUrl":"10.1139/cjm-2024-0064","url":null,"abstract":"Mine environments in boreal and sub-boreal zones are expected to experience extreme weather events, increases in temperature, and shifts in precipitation patterns. Climate change impacts on geochemical stability of tailings contaminants and reclamation structures have been identified as important climate-related challenges to Canadian mining sector. Adapting current reclamation strategies for climate change will improve long-term efficiency and viability of mine tailings remediation/restoration strategies under a changing climate. Accordingly, mesocosm experiments were conducted to investigate associations of climate-driven shifts in microbial communities and functions with changes in the geochemistry of organic covers and underlying tailings. Our results show that warming appears to significantly reduce C:N of organic cover and promote infiltration of nitrogen into deeper, unoxidized strata of underlying tailings. We also observed an increase in the abundance of some nitrate reducers and sulfide oxidizers in microbial communities in underlying tailings. These results raise the concern that warming might trigger oxidation of sulfide minerals (linked to nitrate reduction) in deeper unoxidized strata where the oxygen has been eliminated. Therefore, it would be necessary to have monitoring programs to track functionality of covers in response to climate change conditions. These findings have implications for development of climate resilient mine tailings remediation/restoration strategies.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"470-481"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical-mediated virulence: the effects of host chemicals on microbial virulence and potential new antivirulence strategies. 化学介导的毒力：宿主化学物质对微生物毒力的影响以及潜在的新型抗病毒策略。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-10-01 Epub Date: 2024-06-21 DOI: 10.1139/cjm-2024-0017

Peri B Moulding, Omar M El-Halfawy

{"title":"Chemical-mediated virulence: the effects of host chemicals on microbial virulence and potential new antivirulence strategies.","authors":"Peri B Moulding, Omar M El-Halfawy","doi":"10.1139/cjm-2024-0017","DOIUrl":"10.1139/cjm-2024-0017","url":null,"abstract":"The rising antimicrobial resistance rates and declining antimicrobial discovery necessitate alternative strategies to combat multidrug-resistant pathogens. Targeting microbial virulence is an emerging area of interest. Traditionally, virulence factors were largely restricted to bacteria-derived toxins, adhesins, capsules, quorum sensing systems, secretion systems, factors required to sense, respond to, acquire, or synthesize, and utilize trace elements (such as iron and other metals) and micronutrients (such as vitamins), and other factors bacteria use to establish infection, form biofilms, or damage the host tissues and regulatory elements thereof. However, this traditional definition overlooks bacterial virulence that may be induced or influenced by host-produced metabolites or other chemicals that bacteria may encounter at the infection site. This review will discuss virulence from a non-traditional perspective, shedding light on chemical-mediated host-pathogen interactions and outlining currently available mechanistic insight into increased bacterial virulence in response to host factors. This review aims to define a possibly underestimated theme of chemically mediated host-pathogen interactions and encourage future validation and characterization of the contribution of host chemicals to microbial virulence in vivo. From this perspective, we discuss proposed antivirulence compounds and suggest new potential targets for antimicrobials that prevent chemical-mediated virulence. We also explore proposed host-targeting therapeutics reducing the level of host chemicals that induce microbial virulence, serving as virulence attenuators. Understanding the host chemical-mediated virulence may enable new antimicrobial solutions to fight multidrug-resistant pathogens.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"405-425"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key drivers of antimicrobial resistance in Enterococcus using machine learning. 利用机器学习识别肠球菌耐药性的关键驱动因素。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-10-01 Epub Date: 2024-07-30 DOI: 10.1139/cjm-2024-0049

Jee In Kim, Alexander Manuele, Finlay Maguire, Rahat Zaheer, Tim A McAllister, Robert G Beiko

{"title":"Identification of key drivers of antimicrobial resistance in Enterococcus using machine learning.","authors":"Jee In Kim, Alexander Manuele, Finlay Maguire, Rahat Zaheer, Tim A McAllister, Robert G Beiko","doi":"10.1139/cjm-2024-0049","DOIUrl":"10.1139/cjm-2024-0049","url":null,"abstract":"With antimicrobial resistance (AMR) rapidly evolving in pathogens, quick and accurate identification of genetic determinants of phenotypic resistance is essential for improving surveillance, stewardship, and clinical mitigation. Machine learning (ML) models show promise for AMR prediction in diagnostics but require a deep understanding of internal processes to use effectively. Our study utilised AMR gene, pangenomic, and predicted plasmid features from 647 Enterococcus faecium and Enterococcus faecalis genomes across the One Health continuum, along with corresponding resistance phenotypes, to develop interpretive ML classifiers. Vancomycin resistance could be predicted with 99% accuracy with AMR gene features, 98% with pangenome features, and 96% with plasmid clusters. Top pangenome features overlapped with the resistance genes of the vanA operon, which are often laterally transmitted via plasmids. Doxycycline resistance prediction achieved approximately 92% accuracy with pangenome features, with the top feature being elements of Tn916 conjugative transposon, a tet(M) carrier. Erythromycin resistance prediction models achieved about 90% accuracy, but top features were negatively correlated with resistance due to the confounding effect of population structure. This work demonstrates the importance of reviewing ML models' features to discern biological relevance even when achieving high-performance metrics. Our workflow offers the potential to propose hypotheses for experimental testing, enhancing the understanding of AMR mechanisms, which are crucial for combating the AMR crisis.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"446-460"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Totarol exhibits antibacterial effects through antibiofilm and combined interaction against vancomycin-resistant Enterococcus faecalis. 托塔洛尔通过抗生物膜和联合作用对耐万古霉素粪肠球菌产生抗菌效果。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-10-01 Epub Date: 2024-07-24 DOI: 10.1139/cjm-2024-0014

Ga-Eun Hyeon, Yong-Bin Eom

{"title":"Totarol exhibits antibacterial effects through antibiofilm and combined interaction against vancomycin-resistant Enterococcus faecalis.","authors":"Ga-Eun Hyeon, Yong-Bin Eom","doi":"10.1139/cjm-2024-0014","DOIUrl":"10.1139/cjm-2024-0014","url":null,"abstract":"The rise of vancomycin-resistant enterococci (VRE) due to antibiotic overuse poses a significant threat to long-term care patients and those with impaired immune systems. Therefore, it is imperative to seek alternatives to overcome multidrug resistance. This study aimed to evaluate totarol, a natural compound derived from Podocarpus totara, for its antibacterial activity against vancomycin-resistant Enterococcus faecalis (VREF). Totarol exhibited potent antibacterial activity at a very low concentration of 0.25 µg/mL and demonstrated antibiofilm effects through biofilm inhibitory concentration and biofilm eradication concentration assays. Confocal laser scanning microscopy confirmed that totarol inhibited not only biofilm mass but also bacterial cell viability. The combinatorial use of sublethal concentrations of totarol and vancomycin showed antibacterial activity, as observed in the time-kill assay. Quantitative polymerase chain reaction assays revealed a concentration-dependent downregulation of key virulence genes (vanA, ace, asa, efaA, and esp) in VREF when exposed to totarol. In summary, totarol emerges as a promising adjuvant with vancomycin for inhibiting VREF, addressing vancomycin resistance and biofilm formation-critical challenges associated with VRE infection. Since this was an in vitro study, the role of totarol in the clinical implications of VREF treatment remains to be demonstrated.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"426-432"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of herbaceous litter thickness on bacterial community structure and physicochemical properties of aeolian sand. 草本垃圾厚度对风化沙细菌群落结构和理化特性的影响

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-10-01 Epub Date: 2024-09-10 DOI: 10.1139/cjm-2023-0219

Jieru Kong, Huili Liu, Ruixia Wang, Jun Wang, Pin Zhai, Ruoshi Ma, Peng Kang, Bingru Liu

{"title":"Influence of herbaceous litter thickness on bacterial community structure and physicochemical properties of aeolian sand.","authors":"Jieru Kong, Huili Liu, Ruixia Wang, Jun Wang, Pin Zhai, Ruoshi Ma, Peng Kang, Bingru Liu","doi":"10.1139/cjm-2023-0219","DOIUrl":"10.1139/cjm-2023-0219","url":null,"abstract":"The change and mechanism of soil and soil bacterial diversity during the change of herbaceous litter thickness in desert areas is crucial to understand. In the study, the dominant herbaceous litter mixture in Baijitan National Nature Reserve was selected as the research material, and an experiment was established by adjusting the litter depth. The results showed that the measured values of soil physicochemical factors (total nitrogen, total protein, total potassium, available phosphorus, available potassium, pH, and soil water content) increased with the increase of herbaceous litter mixture thickness in 0-5 cm soil layer. Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, and Gemmatimonadetes were the dominant bacterial phyla under different thicknesses of herbaceous litter in 0-5 and 5-10 cm soil layers. Balneimonas, Rubrobacter, and Geodermatophilus were the dominant bacterial genera under different thicknesses of herbaceous litter in 0-5 and 5-10 cm soil layers. There was no obvious change in the α-diversity index of bacterial community the same soil layer, but the α-diversity index in the 0-5 cm soil layer was lower compared to the 5-10 cm soil layer. The results of this study revealed that the change of herbaceous litter thickness had no significant effect on soil bacterial community structure in desert areas.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"433-445"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constraint on boric acid resistance and tolerance evolvability in Candida albicans. 白念珠菌对硼酸抗性和耐受性进化的制约因素

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-31 DOI: 10.1139/cjm-2023-0225

Yana Syvolos, Ola E Salama, Aleeza C Gerstein

{"title":"Constraint on boric acid resistance and tolerance evolvability in Candida albicans.","authors":"Yana Syvolos, Ola E Salama, Aleeza C Gerstein","doi":"10.1139/cjm-2023-0225","DOIUrl":"10.1139/cjm-2023-0225","url":null,"abstract":"Boric acid is a broad-spectrum antimicrobial used to treat vulvovaginal candidiasis when patients relapse on the primary azole drug fluconazole. Candida albicans is the most common cause of vulvovaginal candidiasis, colloquially referred to as a \"vaginal yeast infection\". Little is known about the propensity of C. albicans to develop BA resistance or tolerance (the ability of a subpopulation to grow slowly in high levels of drug). We evolved 96 replicates from eight diverse C. albicans strains to increasing BA concentrations to test the evolvability of BA resistance and tolerance. Replicate growth was individually assessed daily, with replicates passaged when they had reached an optical density consistent with exponential growth. Many replicates went extinct quickly. Although some replicates could grow in much higher levels of BA than the ancestral strains, evolved populations isolated from the highest terminal BA levels (after 11 weeks of passages) surprisingly showed only modest growth improvements and only at low levels of BA. No large increases in resistance or tolerance were observed in the evolved replicates. Overall, our findings illustrate that there may be evolutionary constraints limiting the emergence of BA resistance and tolerance, which could explain why it remains an effective treatment for recurrent yeast infections.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"384-393"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The limit to evolutionary rescue depends on ploidy in yeast exposed to nystatin. 进化拯救的极限取决于暴露于硝酸菌素的酵母的倍性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1139/cjm-2023-0235

Jasmine Ono, Anastasia Kuzmin, Lesley Miller, Sarah P Otto

{"title":"The limit to evolutionary rescue depends on ploidy in yeast exposed to nystatin.","authors":"Jasmine Ono, Anastasia Kuzmin, Lesley Miller, Sarah P Otto","doi":"10.1139/cjm-2023-0235","DOIUrl":"10.1139/cjm-2023-0235","url":null,"abstract":"The number of copies of each chromosome, or ploidy, of an organism is a major genomic factor affecting adaptation. We set out to determine how ploidy can impact the outcome of evolution, as well as the likelihood of evolutionary rescue, using short-term experiments with yeast (Saccharomyces cerevisiae) in a high concentration of the fungicide nystatin. In similar experiments using haploid yeast, the genetic changes underlying evolutionary rescue were highly repeatable, with all rescued lines containing a single mutation in the ergosterol biosynthetic pathway. All of these beneficial mutations were recessive, which led to the expectation that diploids would find alternative genetic routes to adaptation. To test this, we repeated the experiment using both haploid and diploid strains and found that diploid populations did not evolve resistance. Although diploids are able to adapt at the same rate as haploids to a lower, not fully inhibitory, concentration of nystatin, the present study suggests that diploids are limited in their ability to adapt to an inhibitory concentration of nystatin, while haploids may undergo evolutionary rescue. These results demonstrate that ploidy can tip the balance between adaptation and extinction when organisms face an extreme environmental change.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"394-404"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of a broad host range lytic phage vB_SenS_TUMS_E19 against Salmonella enterica and its efficiency evaluation in the liquid egg. 针对肠炎沙门氏菌的宽宿主范围噬菌体 vB_SenS_TUMS_E19 的特征和基因组分析及其在液态蛋中的效率评估。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-04 DOI: 10.1139/cjm-2024-0013

Narges Torkashvand, Haniyeh Kamyab, Ahmad Reza Shahverdi, Mohammad Reza Khoshayand, Mohammad Amir Karimi Tarshizi, Zargham Sepehrizadeh

{"title":"Characterization and genome analysis of a broad host range lytic phage vB_SenS_TUMS_E19 against Salmonella enterica and its efficiency evaluation in the liquid egg.","authors":"Narges Torkashvand, Haniyeh Kamyab, Ahmad Reza Shahverdi, Mohammad Reza Khoshayand, Mohammad Amir Karimi Tarshizi, Zargham Sepehrizadeh","doi":"10.1139/cjm-2024-0013","DOIUrl":"10.1139/cjm-2024-0013","url":null,"abstract":"Salmonella enterica serovars are zoonotic bacterial that cause foodborne enteritis. Due to bacteria's antibiotic resistance, using bacteriophages for biocontrol and treatment is a new therapeutic approach. In this study, we isolated, characterized, and analyzed the genome of vB_SenS_TUMS_E19 (E19), a broad host range Salmonella bacteriophage, and evaluated the influence of E19 on liquid eggs infected with Salmonella enterica serovar Enteritidis. Transmission electron microscopy showed that the isolated bacteriophage had a siphovirus morphotype. E19 showed rapid adsorption (92% in 5 min), a short latent period (18 min), a large burst size (156 PFU per cell), and a broad host range against different Salmonella enterica serovars. Whole-genome sequencing analysis indicated that the isolated phage had a 42 813 bp long genome with 49.8% G + C content. Neither tRNA genes nor those associated with antibiotic resistance, virulence factors, or lysogenic formation were detected in the genome. The efficacy of E19 was evaluated in liquid eggs inoculated with S. Enteritidis at 4 and 25 °C, and results showed that it could effectively eradicate S. Enteritidis in just 30 min and prevented its growth up to 72 h. Our findings indicate that E19 can be an alternative to a preservative to control Salmonella in food samples and help prevent and treat salmonellosis.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"358-369"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0