Canadian journal of microbiology最新文献_第5页

Host-specific soil oomycete communities are spatially repeatable across natural grasslands. 寄主特有的土壤卵菌群落在自然草原上具有空间可重复性。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2026-0106

Jack Royle, Itaii Applebaum, John Paul Wasan, Sean R Asselin, Jonathan Bennett

{"title":"Host-specific soil oomycete communities are spatially repeatable across natural grasslands.","authors":"Jack Royle, Itaii Applebaum, John Paul Wasan, Sean R Asselin, Jonathan Bennett","doi":"10.1139/cjm-2026-0106","DOIUrl":"10.1139/cjm-2026-0106","url":null,"abstract":"Soil oomycetes contain some of the most devastating plant pathogens, and are widespread within the soil microbiome, yet how individual plant species structure oomycete communities in natural soils, and whether any such structuring is consistent across space, remains poorly resolved. Because host-specific pathogen accumulation is a central prediction of plant-soil feedback theory but is rarely tested outside greenhouse conditions, we focused on whether the host-specific fraction of these communities is preferentially pathogenic. Using oomycete-specific internal transcribed spacer amplicons, we sequenced rhizosphere-associated and paired bulk soils from four grassland species across 25 sites on the Canadian Prairies spanning more than 200 000 km2, totalling 1800 samples. Each host supported a distinctive core of taxa, with two species more likely to host pathogenic taxa unique to their rhizosphere. This enrichment was decoupled from richness of oomycete taxa in the rhizosphere, indicating selective accumulation rather than a by-product of resource-rich environments. Community composition also differed among hosts after accounting for site level edaphic variation. Host plant identity therefore leaves a detectable, spatially repeatable imprint on soil oomycete communities, one expressed most strongly through the selective accumulation of pathogenic taxa.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Shiga toxin-producing Escherichia coli O157:H7 attachment to human intestinal cells by single or combined lytic bacteriophages. 单一或联合噬菌体对产志贺毒素大肠杆菌O157:H7附着人肠细胞的抑制作用

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0172

Akeel Faizal, Yan Dong Niu

{"title":"Inhibition of Shiga toxin-producing Escherichia coli O157:H7 attachment to human intestinal cells by single or combined lytic bacteriophages.","authors":"Akeel Faizal, Yan Dong Niu","doi":"10.1139/cjm-2025-0172","DOIUrl":"10.1139/cjm-2025-0172","url":null,"abstract":"We reported phage cocktails of AHP24 (T1), wV7 (T4), AKFV33 (T5), and AHP24S (rV5) had superior efficacy against STEC O157:H7 strains in broth culture and beef matrices, but it is unknown if they can lyse the pathogens in the context of intestinal epithelial cells, which may reduce Shiga toxin-producing Escherichia coli (STEC) attachment, an initial step for STEC invasion. The objective of this study was to compare efficacy of lytic phages T1, T4, T5, and rV5 as individuals or cocktails in preventing STEC attachment to human intestinal epithelial cells. Two intestinal epithelial cell lines, Caco2 and T84, that are susceptible to STEC attachment were used. There were ∼2-3 log10 colony forming units/mL reductions (P < 0.0001) in STEC attachment when these epithelial cells were exposed to individual or cocktails of phages 1 h before inoculation. The phage cocktail (T5 + T1 + rV5 + T4) significantly reduced STEC attachment onto T84 cells when compared to individual phage treatments T4 and T1 (P < 0.0001). Notably, applying three- (excluding T5) or four-phage cocktails concurrent with STEC inoculation did not significantly different from phage pre-exposure. Phages may be a viable approach for preventing and treating STEC infection in human.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145721261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of marine bacteria through a "bait" approach. 用“诱饵”法分离海洋细菌。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0037

Bahar Pakseresht, Zachary Schiffman, Susan McLatchie, Pascale Coulombe, Safiya Soullane, Anic Imfeld, Yves Gélinas, David A Walsh, Brandon L Findlay

{"title":"Isolation of marine bacteria through a \"bait\" approach.","authors":"Bahar Pakseresht, Zachary Schiffman, Susan McLatchie, Pascale Coulombe, Safiya Soullane, Anic Imfeld, Yves Gélinas, David A Walsh, Brandon L Findlay","doi":"10.1139/cjm-2025-0037","DOIUrl":"10.1139/cjm-2025-0037","url":null,"abstract":"There is a great divide between the microbes active in natural environments and the organisms that may be grown in a laboratory setting. In this work we set out to cultivate representatives of the marine myxobacterial clade, a highly diverse, largely uncultured group of Gram-negative bacteria believed to have extensive biosynthetic potential. Sediment samples were collected from the St. Lawrence Estuary and Gulf and the presence of active marine myxobacteria was established through qPCR analysis of 16S rRNA gene and transcript abundances. In the expectation that the marine myxobacteria would exhibit predatory behaviour like their terrestrial counterparts, the sediment samples were then streaked on agar plates that contained common marine bacteria as the sole carbon source. Unexpectedly, in place of myxobacteria we isolated Pseudomonas, Bacillus, and Stenotropomonas spp., among others, revealing a generalized ability for these strains to break down living organic matter and suggesting that \"bait\" bacteria may be an effective approach for the cultivation of novel marine saprophytes.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145630387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of soil microbiome throughout the cultivation life cycle of Phallus rubrovolvatus. 黄花阳栽培全生命周期土壤微生物组动态。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0279

Xudong Huang, Kairui Deng, Guosheng Zhu, Wanbing Huang, Guanglu Gong, Hongyu Liu, Tongjin Yang, Yang Gui, Wenqiang Li

{"title":"Dynamics of soil microbiome throughout the cultivation life cycle of Phallus rubrovolvatus.","authors":"Xudong Huang, Kairui Deng, Guosheng Zhu, Wanbing Huang, Guanglu Gong, Hongyu Liu, Tongjin Yang, Yang Gui, Wenqiang Li","doi":"10.1139/cjm-2025-0279","DOIUrl":"10.1139/cjm-2025-0279","url":null,"abstract":"Phallus rubrovolvatus is a valuable edible fungus extensively cultivated in Guizhou Province, China. However, the changes in the soil microbiome throughout its growth cycle remain poorly understood. In this study, we collected 35 casing soil samples across five growth stages covering the entire 120-day cultivation cycle of Phallus rubrovolvatus and conducted metagenomic sequencing to examine alterations in soil microbial composition, diversity, key biomarkers, and functional potential. Our analyses revealed significant stage-dependent shifts in microbial community structure, with alpha diversity reaching its lowest at the primordium stage (Shannon of 5.12) and network complexity peaking at harvest stage (1.8-fold increase in connectivity). Through LEfSe analysis, we identified 37 stage-specific microbial biomarkers primarily affiliated with Actinomycetota and Acidobacteriota. Notably, Acidobacteriota biomarkers dominated at the primordium stage, while Nitrospirota enrichment characterized the harvest stage. Functional analyses revealed that membrane transport and energy metabolism pathways were enriched during early mycelial colonization, whereas secondary metabolite biosynthesis and signaling pathways became prominent during fruiting body maturation. Correlation analyses identified available nitrogen as the primary soil variable associated with microbial community composition. These findings provide foundational knowledge of microbiome dynamics during Phallus rubrovolvatus cultivation and suggest that microbiome-based management strategies may benefit from stage-specific interventions synchronized with fungal developmental transitions.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance comparison of commercially available kits for wild birds RNA extraction. 市售野生鸟类RNA提取试剂盒的性能比较。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0213

Ane López-Morales, Vega Alvarez, Xeider Gerrikagoitia, Jose Luis Lavín, Marta Barral

{"title":"Performance comparison of commercially available kits for wild birds RNA extraction.","authors":"Ane López-Morales, Vega Alvarez, Xeider Gerrikagoitia, Jose Luis Lavín, Marta Barral","doi":"10.1139/cjm-2025-0213","DOIUrl":"10.1139/cjm-2025-0213","url":null,"abstract":"RNA extraction demands careful sample handling and processing due to the lack of stability of RNA in the environment. However, these conditions are sometimes challenging to achieve when working with free-ranging animals. The main objective of this study was to evaluate the efficiency of three commercial RNA extraction kits for biological samples obtained from six wild bird species (black-headed gull, griffon vulture, house sparrow, mallard, song thrush, and common starling). We extracted 24 samples (faeces, oropharyngeal swabs, and cloacal swabs) using RNeasy PowerMicrobiome Kit, RNeasy PowerFecal Pro Kit, and RNeasy Mini Kit (Qiagen). The extraction performance was assessed based on total RNA yield, RNA integrity, and purity ratios (260/280 and 260/230). Moreover, an analysis to determine the inhibitory effect of the extracted samples and a multiple linear regression model were performed. RNA extraction performance varied considerably among species, sample types, and extraction kits. Overall, the RNeasy PowerFecal Pro Kit yielded higher RNA integrity and purity values and showed lower quantitative reverse transcription polymerase chain reaction inhibition than the other kits, particularly in faecal samples. Our findings highlight the importance of selecting extraction methods according to both species and sample type when working with wildlife samples.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptidylarginine deiminase inhibitor treatment is associated with increased phage susceptibility in Erwinia amylovora. 肽精氨酸脱亚胺酶抑制剂治疗与淀粉状欧文菌噬菌体敏感性增加有关。

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2026-0025

Nassereldin Ibrahim, Antonet M Svircev, Hany Anany, Joel T Weadge

引用次数: 0

Examining the competitive exclusion and pathogenic potential of Pseudomonadota isolated from healthy chickens. 健康鸡假单胞菌竞争排斥及其致病潜力的研究。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0179

Zhixuan Feng, Jennifer Ronholm

{"title":"Examining the competitive exclusion and pathogenic potential of Pseudomonadota isolated from healthy chickens.","authors":"Zhixuan Feng, Jennifer Ronholm","doi":"10.1139/cjm-2025-0179","DOIUrl":"10.1139/cjm-2025-0179","url":null,"abstract":"The chicken intestine presents a complex environment for microbial survival due to high interbacterial competition, high bile salt concentrations, a low pH, and microaerophilic conditions. While most probiotics contain members of the Bacillota phylum, members of the Pseudomonadota phylum are known to be more important in competitive exclusion-which may be an important consideration in the formulation of future probiotics. Little is known about commensal Pseudomonadota in healthy chickens, or what benefits members of this phylum may offer the host; most studies on Pseudomonadota focus on aspects of opportunistic pathogenesis and dysbiosis. In this study, we use an in silico approach to evaluate the pathogenic potential, competition strategies, and potential host benefits of Pseudomonadota isolates from healthy chickens. We analyzed the draft genomes of 29 representative isolates of Pseudomonadota using Bagle4, AntiSMash, SeCreT6, KEGG mapper, and Virsorter2 to identify key interbacterial competition strategies including secondary metabolite biosynthesis, secretion systems, quorum sensing, and prophages. Our results revealed that each isolate exhibits distinct interbacterial competitive strategies, often independent of their taxonomic affiliation. Including Pseudomonadota in future poultry probiotics may be critical to improving colonization resistance in industrially raised poultry.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145470724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal infection and antibody response to highly pathogenic H5N1 influenza A virus in ducks in the Mississippi and Central Flyways, United States. 美国密西西比和中部飞道鸭的季节性感染和对高致病性H5N1甲型流感病毒的抗体反应

IF 2.4 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0274

David E Stallknecht, Deborah L Carter, Lyndon Sullivan-Brügger, Zijing Cao, Paul Link, Kacie Rome, Mackenzie Kleinpeter, Reid Viegut, Jeffrey Edwards, Jillian Murphy, Ciara McCarty, Bruce Davis, Lynda Knutsen, James Graham, Rebecca L Poulson

{"title":"Seasonal infection and antibody response to highly pathogenic H5N1 influenza A virus in ducks in the Mississippi and Central Flyways, United States.","authors":"David E Stallknecht, Deborah L Carter, Lyndon Sullivan-Brügger, Zijing Cao, Paul Link, Kacie Rome, Mackenzie Kleinpeter, Reid Viegut, Jeffrey Edwards, Jillian Murphy, Ciara McCarty, Bruce Davis, Lynda Knutsen, James Graham, Rebecca L Poulson","doi":"10.1139/cjm-2025-0274","DOIUrl":"10.1139/cjm-2025-0274","url":null,"abstract":"Clade 2.3.4.4b A/goose/Guangdong/1/1996 (Gs/GD) H5N1 highly pathogenic (HP) influenza A virus (IAV) (HP H5N1) infection in Blue-winged Teal in the Mississippi Flyway in the United States during the 2022/2023 and 2023/2024 migratory seasons followed a seasonal pattern similar to low pathogenic IAV, with infection peaks in late summer and fall. To determine if this seasonal pattern persisted during the 2024/2025 migration season, we repeated testing of Blue-winged Teal from September 2024 to April 2025 in the Mississippi Flyway. Results from other dabbling duck species sampled concurrently with Blue-winged Teal during the 2023/2024 and 2024/2025 migratory seasons in the Mississippi and Central Flyways also were compared. For Blue-winged Teal in the Mississippi Flyway, a seasonal peak in infection was detected during late fall (November/December 2024) on wintering grounds in Louisiana and Texas corresponding to low H5 and N1 antibody prevalence in juvenile birds. Estimated antibody prevalence increased rapidly following this detection, and from January to April 2025, HP H5N1 infections were not detected. Virologic and serologic results in Mallard, Northern Pintail, American Wigeon, Northern Shoveler, and Green-winged Teal mirrored virologic and serologic results observed in Blue-winged Teal and were consistent between flyways.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retention of entire genomes in Plasmodiophora brassica represents a previously unrecognized mechanism of balancing selection. 在芸苔Plasmodiophora brassica中，整个基因组的保留代表了一种以前未被认识到的平衡选择机制。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0249

Bruce D Gossen, Afsaneh Sedaghatkish, Mary Ruth McDonald

{"title":"Retention of entire genomes in Plasmodiophora brassica represents a previously unrecognized mechanism of balancing selection.","authors":"Bruce D Gossen, Afsaneh Sedaghatkish, Mary Ruth McDonald","doi":"10.1139/cjm-2025-0249","DOIUrl":"10.1139/cjm-2025-0249","url":null,"abstract":"A novel mechanism of balancing selection, a type of natural selection, in root pathogen Plasmodiophora brassicae, is presented. Recent studies have demonstrated that Plasmodiophora brassicae retains entire genomes in its population over time. The proposed mechanism for this retention is suppression of host resistance during infection by a virulent genotype (pathotype). This suppression of resistance provides an opportunity for infection by avirulent pathotypes, which would otherwise not be able to infect and reproduce in the resistant host cultivar. In this novel mechanism, which we propose to name as beta retention, a virulent genotype (alpha genotype) opens the door for infection by avirulent genotype (beta genotypes). Under other conditions, such as a different resistant cultivar or host, the alpha genotype could function as a beta genotype and vice versa. This novel mechanism of balancing selection is distinct from existing mechanisms in that entire genomes are retained in the population; other mechanisms maintain single genes or gene clusters. Retention of whole genomes has not previously been reported, probably because it occurs only in microbial plant pathogens and the beta genotypes could only be identified using whole-genome sequences. Beta retention is proposed as a separate mechanism of balancing selection.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Movements of poultry product off HPAI-infected premises: using science-based standards for achieving confidence in risk. 家禽产品从感染禽流感的场所转移：使用基于科学的标准实现对风险的信心。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0202

Mickey Leonard, Peter Bonney, Carol Cardona, Amos Ssematimba, Kaitlyn St Charles, Sylvia Wanzala Martin, Catherine Alexander, Rosemary Marusak

{"title":"Movements of poultry product off HPAI-infected premises: using science-based standards for achieving confidence in risk.","authors":"Mickey Leonard, Peter Bonney, Carol Cardona, Amos Ssematimba, Kaitlyn St Charles, Sylvia Wanzala Martin, Catherine Alexander, Rosemary Marusak","doi":"10.1139/cjm-2025-0202","DOIUrl":"10.1139/cjm-2025-0202","url":null,"abstract":"During highly pathogenic avian influenza (HPAI) outbreaks, the United States Department of Agriculture response requires infected egg production premises to discard on-site poultry products, including eggs that have been processed and stored prior to infection of the site. The disposal of these eggs contributes to global food insecurity through market disruptions, industry revenue loss, and federal indemnity paid. Further, rural farming communities are economically destabilized and farmer health and well-being challenged. To support continuity of business movement decisions during HPAI outbreaks, the Secure Poultry Supply team at the University of Minnesota along with an egg sector stakeholder workgroup has been examining the risk of moving to market processed, segregated, and stored eggs from an infected premises that were laid by uninfected flocks (i.e., a group of egg-laying hens kept in one house/barn during their entire lay cycle). Three important criteria for achieving both acceptable risk and confidence for movement were identified: identification and removal of potentially contaminated eggs from the cooler; protection of cooler eggs from recontamination; and science-assessed egg truck disinfection protocols for exiting an infected premises. The science behind biosecurity and biocontainment practices and risk determination for moving eggs off infected premises are discussed. Confidence in movements determined to be low risk may provide a means for farm and food-protein sustainability.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145818146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0