Canadian journal of microbiology最新文献_第4页

High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water. 高通量测序揭示了两种油田采出水处理系统中的微生物群落动态。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0202

Yi Li, Shuo Han, Xiangwei He

{"title":"High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water.","authors":"Yi Li, Shuo Han, Xiangwei He","doi":"10.1139/cjm-2024-0202","DOIUrl":"10.1139/cjm-2024-0202","url":null,"abstract":"Petroleum-associated water harbors diverse microbial communities, including hydrocarbon-degrading bacteria, sulfate-reducing bacteria, and methanogenic archaea. The growth and metabolism of these organisms, as well as their community composition, can affect various aspects of oil field development and oil produced water treatment. In this study, Illumina-based 16S rRNA gene sequencing was used to analyze the microbial community structures of oilfield produced water processed at two treatment stations and subjected to different treatment protocols. Significant differences in microbial community α-diversity and richness resulted from the different treatment protocols. The treatment of oil produced water effectively reduced the oil content, accompanied by the a reduction in Desulfobacterota. Proteobacteria was the dominant phylum in oil produced water; its core presence, along with Patescibacteria and Desulfobacterota, was identified in a co-occurrence network analysis of the microbial community. Redundancy analysis showed significant positive correlations between microbial community diversity and the oil and suspended solids contents of the oil produced water, highlighting the role of treatment protocols in shaping both microbial composition and water characteristics. Thus, this study provides potential insights into the processes of souring in oil fields and contributes to the theoretical understanding of oil-produced water treatment, which may inform future optimization of treatment protocols.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the multifaceted roles of Trichoderma secondary metabolites. 探索木霉次生代谢产物的多方面作用。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2025-0045

Kavita Yadav, Prashant Khare

引用次数: 0

Note of appreciation. 表示感谢。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0228

引用次数: 0

Mechanisms and implications of phenotypic switching in bacterial pathogens. 细菌病原体表型转换的机制和影响

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-03 DOI: 10.1139/cjm-2024-0116

Alexander Stephen Byrne, Nathalie Bissonnette, Kapil Tahlan

{"title":"Mechanisms and implications of phenotypic switching in bacterial pathogens.","authors":"Alexander Stephen Byrne, Nathalie Bissonnette, Kapil Tahlan","doi":"10.1139/cjm-2024-0116","DOIUrl":"10.1139/cjm-2024-0116","url":null,"abstract":"Bacteria encounter various stressful conditions within a variety of dynamic environments, which they must overcome for survival. One way they achieve this is by developing phenotypic heterogeneity to introduce diversity within their population. Such distinct subpopulations can arise through endogenous fluctuations in regulatory components, wherein bacteria can express diverse phenotypes and switch between them, sometimes in a heritable and reversible manner. This switching may also lead to antigenic variation, enabling pathogenic bacteria to evade the host immune response. Therefore, phenotypic heterogeneity plays a significant role in microbial pathogenesis, immune evasion, antibiotic resistance, host niche tissue establishment, and environmental persistence. This heterogeneity can result from stochastic and responsive switches, as well as various genetic and epigenetic mechanisms. The development of phenotypic heterogeneity may create clonal populations that differ in their level of virulence, contribute to the formation of biofilms, and allow for antibiotic persistence within select morphological variants. This review delves into the current understanding of the molecular switching mechanisms underlying phenotypic heterogeneity, highlighting their roles in establishing infections caused by select bacterial pathogens.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-19"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surveillance of Clostridioides difficile in Canadian retail meat and genomic linkages to community-associated human clinical infections in Canada. 加拿大零售肉类中艰难梭菌的监测以及与加拿大社区相关人类临床感染的基因组联系。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0193

Paula E Pidsadny, Tim Du, Romeo Hizon, Sean Ahmed, Derek Tan, George G Zhanel, Denice C Bay, Richard J Reid-Smith, Audrey Charlebois, George R Golding

{"title":"Surveillance of Clostridioides difficile in Canadian retail meat and genomic linkages to community-associated human clinical infections in Canada.","authors":"Paula E Pidsadny, Tim Du, Romeo Hizon, Sean Ahmed, Derek Tan, George G Zhanel, Denice C Bay, Richard J Reid-Smith, Audrey Charlebois, George R Golding","doi":"10.1139/cjm-2024-0193","DOIUrl":"10.1139/cjm-2024-0193","url":null,"abstract":"Community-associated Clostridioides difficile infections (CA-CDI) remain a concern in Canada, comprising a quarter of cases previously reported through the Canadian Nosocomial Infection Surveillance Program. Previous Canadian studies have reported toxigenic C. difficile isolated from Canadian retail meat, suggesting that it may be a source of exposure for CA-CDI in Canada. In this study, 3/219 (1.4%) of retail pork and 0/99 (0%) of retail beef samples tested positive for toxigenic C. difficile, which were molecularly characterized by PCR ribotyping and whole-genome sequencing. All three isolates were obtained from pork and belonged to sequence types (STs)/ribotypes (RTs) that have previously been isolated from human clinical CA-CDI cases in Canada: ST1/RT027, ST8/RT002, and ST10/RT015. Retail meat isolates were susceptible to the antimicrobials tested, save one isolate with intermediate resistance to clindamycin. Genomic comparison to Canadian human clinical CA-CDI isolates with the same corresponding ST/RT types showed two of the three pork isolates clustered with CA-CDI isolates via core-genome multilocus sequencing typing, with single nucleotide variant (SNV) analysis showing further genomic relatedness of 2-11 SNVs. Retail meat may therefore be a low source of CA-CDI exposure in Canada, with the potential for foodborne transmission of select clones.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-7"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus suis serovar 9 responses to elevated temperature and co-culture with Glaesserella parasuis. 猪链球菌血清型9对高温和副猪小芽孢杆菌共培养的反应。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0180

B S Spoja, A R Bujold, J I MacInnes, N Ricker

{"title":"Streptococcus suis serovar 9 responses to elevated temperature and co-culture with Glaesserella parasuis.","authors":"B S Spoja, A R Bujold, J I MacInnes, N Ricker","doi":"10.1139/cjm-2024-0180","DOIUrl":"10.1139/cjm-2024-0180","url":null,"abstract":"Streptococcus suis and Glaesserella parasuis are commensal organisms that can shift from a benign to pathogenic state and cause severe disease in swine. We hypothesized that a change in host temperature and/or interactions with G. parasuis could impact S. suis growth dynamics. We compared phenotypic properties of a clinical S. suis serovar 9 strain (SS9C) with clinical serovar 2 and healthy serovar 9 isolates grown at 37 and 41 °C. We further investigated how co-culturing with G. parasuis affected biofilm formation of SS9C. Crystal violet staining indicated that SS9C produced significantly more biofilm than the other strains when grown at 37 °C; this difference was amplified at 41 °C. However, cell counts did not increase at the higher temperature. Biofilms of SS9C at 37 and 41 °C were unaffected by DNase I digestion, while other strains were both susceptible at 41 °C. All biofilms were susceptible to proteinase K and α-amylase digestion at both temperatures. We showed that growth at 41 °C increased biofilm formation and shifted the phenotype of SS9C; however, neither increased temperature nor co-culture with G. parasuis increased planktonic or sessile cell counts. Our study suggests that increased temperature in the host may be an important factor in understanding S. suis disease development.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the occurrence of antimicrobial resistance in the environment in Canada: a scoping review. 调查加拿大环境中抗菌素耐药性的发生：范围审查。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0189

Manuel Pérez Maldonado, Daniel Ofori-Darko, Vanessa Nichols, Jessica French, Kelsey Spence, Richard J Reid-Smith, E Jane Parmley

{"title":"Investigating the occurrence of antimicrobial resistance in the environment in Canada: a scoping review.","authors":"Manuel Pérez Maldonado, Daniel Ofori-Darko, Vanessa Nichols, Jessica French, Kelsey Spence, Richard J Reid-Smith, E Jane Parmley","doi":"10.1139/cjm-2024-0189","DOIUrl":"10.1139/cjm-2024-0189","url":null,"abstract":"Antimicrobial resistance is an environmental, agricultural, and public health problem that is impacting the health of humans and animals. The role of the environment as a source of and transmission pathway for antibiotic resistant bacteria and antibiotic resistance genes is a topic of increasing interest that, to date, has received limited attention. This study aimed to describe the sources and possible pathways contributing to antimicrobial resistance dissemination through bioaerosols, water, and soil in Canada using a scoping review methodology and systems thinking approach. A systems map was created to describe the occurrence and relationships between sources and pathways for antimicrobial resistance dissemination through water, soil, and bioaerosols. The map guided the development of the scoping review protocol, specifically the keywords searched and what data were extracted from the included studies. In total, 103 studies of antimicrobial resistance in water, 67 in soil, and 12 in air were identified. Studies to detect the presence of antimicrobial resistance genes have mainly been conducted at wastewater treatment plants and commercial animal livestock facilities. We also identified elements in the systems map with little or no data available (e.g., retail) that need to be investigated further to have a better understanding of antimicrobial resistance dissemination through different Canadian environments.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morchella capensis sp. nov., the Fynbos morel-the first description of an endemic Morchella species from Africa. 羊肚菌capensis sp. nov.， Fynbos羊肚菌-非洲特有羊肚菌的首次描述。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2023-0224

B Van der Merwe, J Williams, E Le Roux, A Van Staden, G Lawrence, K Jacobs

引用次数: 0

BEVA2.0: modular assembly of golden gate-compatible vectors with expanded utility for genetic engineering. BEVA2.0：金门兼容载体的模块化组装与扩展的基因工程实用程序。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0246

Barney A Geddes, Riley Williamson, Jake Schumacher, Ahmad Ardi, Garrett Levin, Emily Červenka, Rui Huang, George C diCenzo

引用次数: 0

Survival of antimicrobial resistant Salmonella Heidelberg inoculated into microcosms of fresh pine wood shavings for broiler litter. 接种到肉鸡粪便新鲜松木刨花微生态系统中的耐抗菌素海德堡沙门氏菌的存活率

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-22 DOI: 10.1139/cjm-2024-0088

Adelumola Oladeinde, Kimberly Cook, Attiq Rehman, Catherine D Carrillo, Reed Woyda, Crystal Wiersma, Zaid Abdo, Jasmine Johnson, Anna Marie Bosch, Michael Rothrock, Moussa S Diarra

引用次数: 0