Canadian journal of microbiology最新文献_第4页

VeA is involved in anti-tumor activity by regulating adenylosuccinate lyase to mediate the synthesis of Acadesine in endophytic Fusarium solani. VeA通过调节内生镰刀菌中腺苷琥珀酸裂解酶介导乙酰胆碱的合成，参与抗肿瘤活性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0073

Rongfei Liu, Wen Zheng, Lu Cai, Qing Xiao, Guihua Liu, Yuling Jiang, Zhangjiang He, Jichuan Kang

{"title":"VeA is involved in anti-tumor activity by regulating adenylosuccinate lyase to mediate the synthesis of Acadesine in endophytic Fusarium solani.","authors":"Rongfei Liu, Wen Zheng, Lu Cai, Qing Xiao, Guihua Liu, Yuling Jiang, Zhangjiang He, Jichuan Kang","doi":"10.1139/cjm-2024-0073","DOIUrl":"https://doi.org/10.1139/cjm-2024-0073","url":null,"abstract":"Acadesine (AICAR) is a promising candidate for new drugs in Phase III clinical trials. The purpose of this study is to analyse the steps in the biosynthesis pathway of AICAR. Our previous study found that overexpression of veA, a gene encoding a global regulator, significantly increased AICAR production of endophytic Fusarium solani HB1-J1 and the anti-tumor activity of its extracts. Transcriptome and metabolome analysis of FsveAOE14, a veA overexpressing F. solani strain, revealed a 10-step AICAR synthesis pathway, with adenylosuccinate lyase PurB as a key enzyme. Generally, overexpressing purB (the gene encoding adenylosuccinate lyase) enhances AICAR synthesis. However, in FsveAOE14, despite down-regulation of purB, AICAR content increased, which is contradictory. Further studies revealed that expression levels of purB homologs gene, pro06469 and pro10879, were upregulated in FsveAOE14. This suggests that although veA overexpression leads to purB down-regulation, their up-regulation may compensate for the reduction of purB, thus affecting AICAR synthesis. Additionally, compared to the wild type, overexpressing purB significantly enhances the inhibitory activity of the strain's extracts against the nonsmall-cell lung cancer cell line A549. Furthermore, it also increases the metabolic levels of other anti-tumor compounds, including 3-methyladenine, taurine, and others. These results indicate that VeA regulates AICAR biosynthesis via key enzymes like PurB, enhancing AICAR and other anti-tumor compound production, thus increasing the anti-tumor activity of F. solani extracts.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"71 ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Somatic coliphages as bioindicators of contamination in Lake Guamuez, Colombia. 作为哥伦比亚瓜穆埃斯湖污染生物指标的体细胞噬菌体。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-22 DOI: 10.1139/cjm-2023-0147

Mario Andres Pantoja-España, Miller Cerón Gómez, Eddy Patricia López Molano, Raúl Mauricio Insuasty, Eduardo Ibarguen-Mondragón, María Alejandra Mármol Martínez, Deisy Lorena Guerrero-Ceballos, Jenny Dimelza Gómez Arrieta, Juan Camilo Paz García, Pablo Heyman Fernández Izquierdo, Arsenio Hidalgo Troya, Sandra Jaqueline Mena-Huertas, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Edith Mariela Burbano-Rosero

{"title":"Somatic coliphages as bioindicators of contamination in Lake Guamuez, Colombia.","authors":"Mario Andres Pantoja-España, Miller Cerón Gómez, Eddy Patricia López Molano, Raúl Mauricio Insuasty, Eduardo Ibarguen-Mondragón, María Alejandra Mármol Martínez, Deisy Lorena Guerrero-Ceballos, Jenny Dimelza Gómez Arrieta, Juan Camilo Paz García, Pablo Heyman Fernández Izquierdo, Arsenio Hidalgo Troya, Sandra Jaqueline Mena-Huertas, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Edith Mariela Burbano-Rosero","doi":"10.1139/cjm-2023-0147","DOIUrl":"10.1139/cjm-2023-0147","url":null,"abstract":"Lake Guamuez is the second largest lake in Colombia and economically supports hundreds of families in the area. The main activities carried out in the region have focused on tourism, agriculture, livestock, and rainbow trout production; however, these activities have been associated with contamination of the lake. This research aimed to evaluate the water quality of Lake Guamuez using somatic coliphages (SCs) as bioindicators. For this purpose, periodic sampling was carried out for 6 months at nine strategic points of the lake. For the detection of SCs, the method described in 9211 D of the Standard Methods for the Examination of Water and Wastewater was used. The genomic variability and presence of virulence genes in the isolated SCs were determined. Water contamination in the lake is evident, and the SCs titer is greater in areas with a high flow of anthropogenic activities. An important degree of genetic diversity and a high prevalence of virulence genes could be observed among the SCs analyzed. The results when compared with guidelines and water quality standards from various countries showed concentrations of SCs higher than those allowed. The high prevalence of gastrointestinal diseases in the region suggests a link to water contamination.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-14"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative mitogenomics of Leptographium procerum, Leptographium terebrantis, and Leptographium wingfieldii, an invasive fungal species in Canadian forests. 加拿大森林中入侵真菌种细纹细纹、细纹细纹细纹和细纹细纹细纹的有丝分裂基因组学比较。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1139/cjm-2024-0179

Abdullah Zubaer, Alvan Wai, Georg Hausner

{"title":"Comparative mitogenomics of Leptographium procerum, Leptographium terebrantis, and Leptographium wingfieldii, an invasive fungal species in Canadian forests.","authors":"Abdullah Zubaer, Alvan Wai, Georg Hausner","doi":"10.1139/cjm-2024-0179","DOIUrl":"10.1139/cjm-2024-0179","url":null,"abstract":"Leptographium wingfieldii is a fungal associate of Tomicus piniperda (the pine shoot beetle) and pathogen of pines and this species is an agent of blue stain in sapwood on infected trees. This fungus was first reported from Europe and has been recently introduced to Canadian forests. Ten new mitogenomes have been sequenced and characterized, including seven strains of L. wingfieldii, two strains of L. procerum and one strain of L. terebrantis. The data were combined with other members of the Ophiostomatales collected from NCBI to gain more insight into the genetic diversity, evolution, and systematics of these fungi. The size of the studied mitogenomes of Leptographium species ranged from 41 to 126 kb with the number of potential mobile introns embedded within these mitogenomes ranging from 13 to 45. These data show that introns generate genetic diversity and confirms the contribution of mobile introns in genome expansion in Ophiostomatales fungi. This study also uncovered complex intron arrangements (twintrons) suggesting the potential of mobile introns generating complex ribozymes that may have implications in gene regulation.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water. 高通量测序揭示了两种油田采出水处理系统中的微生物群落动态。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0202

Yi Li, Shuo Han, Xiangwei He

{"title":"High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water.","authors":"Yi Li, Shuo Han, Xiangwei He","doi":"10.1139/cjm-2024-0202","DOIUrl":"10.1139/cjm-2024-0202","url":null,"abstract":"Petroleum-associated water harbors diverse microbial communities, including hydrocarbon-degrading bacteria, sulfate-reducing bacteria, and methanogenic archaea. The growth and metabolism of these organisms, as well as their community composition, can affect various aspects of oil field development and oil produced water treatment. In this study, Illumina-based 16S rRNA gene sequencing was used to analyze the microbial community structures of oilfield produced water processed at two treatment stations and subjected to different treatment protocols. Significant differences in microbial community α-diversity and richness resulted from the different treatment protocols. The treatment of oil produced water effectively reduced the oil content, accompanied by the a reduction in Desulfobacterota. Proteobacteria was the dominant phylum in oil produced water; its core presence, along with Patescibacteria and Desulfobacterota, was identified in a co-occurrence network analysis of the microbial community. Redundancy analysis showed significant positive correlations between microbial community diversity and the oil and suspended solids contents of the oil produced water, highlighting the role of treatment protocols in shaping both microbial composition and water characteristics. Thus, this study provides potential insights into the processes of souring in oil fields and contributes to the theoretical understanding of oil-produced water treatment, which may inform future optimization of treatment protocols.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Note of appreciation. 表示感谢。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0228

引用次数: 0

Mechanisms and implications of phenotypic switching in bacterial pathogens. 细菌病原体表型转换的机制和影响

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-03 DOI: 10.1139/cjm-2024-0116

Alexander Stephen Byrne, Nathalie Bissonnette, Kapil Tahlan

{"title":"Mechanisms and implications of phenotypic switching in bacterial pathogens.","authors":"Alexander Stephen Byrne, Nathalie Bissonnette, Kapil Tahlan","doi":"10.1139/cjm-2024-0116","DOIUrl":"10.1139/cjm-2024-0116","url":null,"abstract":"Bacteria encounter various stressful conditions within a variety of dynamic environments, which they must overcome for survival. One way they achieve this is by developing phenotypic heterogeneity to introduce diversity within their population. Such distinct subpopulations can arise through endogenous fluctuations in regulatory components, wherein bacteria can express diverse phenotypes and switch between them, sometimes in a heritable and reversible manner. This switching may also lead to antigenic variation, enabling pathogenic bacteria to evade the host immune response. Therefore, phenotypic heterogeneity plays a significant role in microbial pathogenesis, immune evasion, antibiotic resistance, host niche tissue establishment, and environmental persistence. This heterogeneity can result from stochastic and responsive switches, as well as various genetic and epigenetic mechanisms. The development of phenotypic heterogeneity may create clonal populations that differ in their level of virulence, contribute to the formation of biofilms, and allow for antibiotic persistence within select morphological variants. This review delves into the current understanding of the molecular switching mechanisms underlying phenotypic heterogeneity, highlighting their roles in establishing infections caused by select bacterial pathogens.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-19"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surveillance of Clostridioides difficile in Canadian retail meat and genomic linkages to community-associated human clinical infections in Canada. 加拿大零售肉类中艰难梭菌的监测以及与加拿大社区相关人类临床感染的基因组联系。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0193

Paula E Pidsadny, Tim Du, Romeo Hizon, Sean Ahmed, Derek Tan, George G Zhanel, Denice C Bay, Richard J Reid-Smith, Audrey Charlebois, George R Golding

{"title":"Surveillance of Clostridioides difficile in Canadian retail meat and genomic linkages to community-associated human clinical infections in Canada.","authors":"Paula E Pidsadny, Tim Du, Romeo Hizon, Sean Ahmed, Derek Tan, George G Zhanel, Denice C Bay, Richard J Reid-Smith, Audrey Charlebois, George R Golding","doi":"10.1139/cjm-2024-0193","DOIUrl":"10.1139/cjm-2024-0193","url":null,"abstract":"Community-associated Clostridioides difficile infections (CA-CDI) remain a concern in Canada, comprising a quarter of cases previously reported through the Canadian Nosocomial Infection Surveillance Program. Previous Canadian studies have reported toxigenic C. difficile isolated from Canadian retail meat, suggesting that it may be a source of exposure for CA-CDI in Canada. In this study, 3/219 (1.4%) of retail pork and 0/99 (0%) of retail beef samples tested positive for toxigenic C. difficile, which were molecularly characterized by PCR ribotyping and whole-genome sequencing. All three isolates were obtained from pork and belonged to sequence types (STs)/ribotypes (RTs) that have previously been isolated from human clinical CA-CDI cases in Canada: ST1/RT027, ST8/RT002, and ST10/RT015. Retail meat isolates were susceptible to the antimicrobials tested, save one isolate with intermediate resistance to clindamycin. Genomic comparison to Canadian human clinical CA-CDI isolates with the same corresponding ST/RT types showed two of the three pork isolates clustered with CA-CDI isolates via core-genome multilocus sequencing typing, with single nucleotide variant (SNV) analysis showing further genomic relatedness of 2-11 SNVs. Retail meat may therefore be a low source of CA-CDI exposure in Canada, with the potential for foodborne transmission of select clones.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-7"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus suis serovar 9 responses to elevated temperature and co-culture with Glaesserella parasuis. 猪链球菌血清型9对高温和副猪小芽孢杆菌共培养的反应。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0180

B S Spoja, A R Bujold, J I MacInnes, N Ricker

{"title":"Streptococcus suis serovar 9 responses to elevated temperature and co-culture with Glaesserella parasuis.","authors":"B S Spoja, A R Bujold, J I MacInnes, N Ricker","doi":"10.1139/cjm-2024-0180","DOIUrl":"10.1139/cjm-2024-0180","url":null,"abstract":"Streptococcus suis and Glaesserella parasuis are commensal organisms that can shift from a benign to pathogenic state and cause severe disease in swine. We hypothesized that a change in host temperature and/or interactions with G. parasuis could impact S. suis growth dynamics. We compared phenotypic properties of a clinical S. suis serovar 9 strain (SS9C) with clinical serovar 2 and healthy serovar 9 isolates grown at 37 and 41 °C. We further investigated how co-culturing with G. parasuis affected biofilm formation of SS9C. Crystal violet staining indicated that SS9C produced significantly more biofilm than the other strains when grown at 37 °C; this difference was amplified at 41 °C. However, cell counts did not increase at the higher temperature. Biofilms of SS9C at 37 and 41 °C were unaffected by DNase I digestion, while other strains were both susceptible at 41 °C. All biofilms were susceptible to proteinase K and α-amylase digestion at both temperatures. We showed that growth at 41 °C increased biofilm formation and shifted the phenotype of SS9C; however, neither increased temperature nor co-culture with G. parasuis increased planktonic or sessile cell counts. Our study suggests that increased temperature in the host may be an important factor in understanding S. suis disease development.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the occurrence of antimicrobial resistance in the environment in Canada: a scoping review. 调查加拿大环境中抗菌素耐药性的发生：范围审查。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0189

Manuel Pérez Maldonado, Daniel Ofori-Darko, Vanessa Nichols, Jessica French, Kelsey Spence, Richard J Reid-Smith, E Jane Parmley

{"title":"Investigating the occurrence of antimicrobial resistance in the environment in Canada: a scoping review.","authors":"Manuel Pérez Maldonado, Daniel Ofori-Darko, Vanessa Nichols, Jessica French, Kelsey Spence, Richard J Reid-Smith, E Jane Parmley","doi":"10.1139/cjm-2024-0189","DOIUrl":"10.1139/cjm-2024-0189","url":null,"abstract":"Antimicrobial resistance is an environmental, agricultural, and public health problem that is impacting the health of humans and animals. The role of the environment as a source of and transmission pathway for antibiotic resistant bacteria and antibiotic resistance genes is a topic of increasing interest that, to date, has received limited attention. This study aimed to describe the sources and possible pathways contributing to antimicrobial resistance dissemination through bioaerosols, water, and soil in Canada using a scoping review methodology and systems thinking approach. A systems map was created to describe the occurrence and relationships between sources and pathways for antimicrobial resistance dissemination through water, soil, and bioaerosols. The map guided the development of the scoping review protocol, specifically the keywords searched and what data were extracted from the included studies. In total, 103 studies of antimicrobial resistance in water, 67 in soil, and 12 in air were identified. Studies to detect the presence of antimicrobial resistance genes have mainly been conducted at wastewater treatment plants and commercial animal livestock facilities. We also identified elements in the systems map with little or no data available (e.g., retail) that need to be investigated further to have a better understanding of antimicrobial resistance dissemination through different Canadian environments.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome profiling suggests novel endosymbiont associations of insect pests of stored grain. 微生物组分析表明，贮藏谷物的害虫与新的内共生体有关联。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-11-19 DOI: 10.1139/cjm-2024-0095

Janice Fajardo, Brian Harrison, Vincent A D Hervet, Matthew G Bakker

{"title":"Microbiome profiling suggests novel endosymbiont associations of insect pests of stored grain.","authors":"Janice Fajardo, Brian Harrison, Vincent A D Hervet, Matthew G Bakker","doi":"10.1139/cjm-2024-0095","DOIUrl":"10.1139/cjm-2024-0095","url":null,"abstract":"Many arthropods, including economically important pests of stored grains, host intracellular bacterial symbionts. These symbionts can have diverse impacts on host morphology, stress tolerance, and reproductive success. The ability to rapidly determine the infection status of host insects and the identity of intracellular symbionts, if present, is vital to understanding the biology and ecology of these organisms. We used a microbiome profiling method based on amplicon sequencing to rapidly screen 35 captive insect colonies. This method effectively revealed single and mixed infections by intracellular bacterial symbionts, as well as the presence or absence of a dominant symbiont, when that was the case. Because no a priori decisions are required about probable host-symbiont pairing, this method is able to quickly identify novel associations. This work highlights the frequency of endosymbionts, indicates some unexpected pairings that should be investigated further, such as dominant bacterial taxa that are not among the canonical genera of endosymbionts, and reveals different colonies of the same host insect species that differ in the presence and identity of endosymbiotic bacteria.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-6"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0