Canadian journal of microbiology最新文献_第4页

A qualitative scan on the challenges of AMR in Canada and experts' proposed solutions. 对加拿大抗菌素耐药性的挑战和专家提出的解决方案进行定性分析。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0143

Armelle Lorcy, Antoine Garnier, Peter Daley, Ève Dubé

{"title":"A qualitative scan on the challenges of AMR in Canada and experts' proposed solutions.","authors":"Armelle Lorcy, Antoine Garnier, Peter Daley, Ève Dubé","doi":"10.1139/cjm-2025-0143","DOIUrl":"10.1139/cjm-2025-0143","url":null,"abstract":"Antimicrobial resistance (AMR) is a growing public health threat in Canada. In 2022, rising resistance was reported among key pathogens, yet national coordination remains inconsistent, with strategies varying widely by province and territory. This study explored AMR-related challenges in healthcare and the impact of the COVID-19 pandemic on AMR efforts in Canada. Using a qualitative design, researchers conducted semi-structured interviews with 59 experts from fields such as microbiology, public health, and industry. Participants were identified through environmental scanning and snowball sampling. Thematic analysis of transcripts revealed major barriers to a unified AMR response, including inconsistent surveillance, fragmented stewardship efforts, and decentralized health systems. According to participating AMR experts, COVID-19 disrupted AMR control by diverting resources and potentially increasing resistance but also led to improvements in infection prevention, public awareness, and health infrastructure. Participants emphasized the need for stronger political commitment, improved surveillance, a One Health approach, and better funded, coordinated stewardship programs. With the publication of the Pan-Canadian Action Plan in 2023, the Canadian federal government has announced changes. However, effectively addressing AMR will require a unified, multisectoral strategy that bridges political, health, and societal efforts across jurisdictions.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145556250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of transmission metrics in a slow-spreading highly pathogenic avian influenza (HPAI) outbreak in a commercial upland game bird system. 高原商业猎禽系统中一次慢传播高致病性禽流感（HPAI）暴发的传播指标评估。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0203

Kaitlyn M St Charles, Amos Ssematimba, Peter J Bonney, Carol J Cardona

{"title":"Evaluation of transmission metrics in a slow-spreading highly pathogenic avian influenza (HPAI) outbreak in a commercial upland game bird system.","authors":"Kaitlyn M St Charles, Amos Ssematimba, Peter J Bonney, Carol J Cardona","doi":"10.1139/cjm-2025-0203","DOIUrl":"10.1139/cjm-2025-0203","url":null,"abstract":"In 2022, highly pathogenic avian influenza virus (HPAIV) H5N1 clade 2.3.4.4b was detected in United States (US) poultry, quickly escalating into an outbreak that surpassed the 2014/15 HPAI US event in scale and impact. Unlike in 2014/15, the 2022/3/4/5 outbreak has included numerous HPAI detections in previously infrequently affected commodities such as broilers and commercially-raised upland game birds. Here, we describe H5 HPAI detections that occurred between December 2023 and January 2024 in a multipremises, commerical upland game bird system located in the Midwestern US. We used an approximate Bayesian computation algorithm and stochastic within-flock HPAI transmission model to estimate the following: (1) time of HPAI introduction onto each affected premises, (2) number of days between estimated times of introduction and times of detection, and (3) the adequate contact rates and basic reproduction numbers within individual barns. Clinical signs and mortality observed in the infected pheasant flocks were largely consistent with other pheasant H5 clade 2.3.4.4b outbreaks. Across the system, the estimated transmission metrics were noticeably lower than those calculated from outbreaks in other poultry species. However, times to detection were similar to HPAI outbreaks that have occurred in other commodities.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly pathogenic avian influenza (HPAI H5N1, clade 2.3.4.4b, genotype C2.1) in commercial ring-necked pheasants during the 2022 outbreak in the United States. 2022年美国商业环颈雉爆发高致病性禽流感（HPAI H5N1，分支2.3.4.4b，基因型C2.1）

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0198

Kayla R Niel, Kaitlyn M St Charles, Jonathan Y Elissa, Megan E Lighty, Amos Ssematimba, Marie Culhane, Alvin Crespo-Bellido, Nídia S Trovão, Martha I Nelson, Carol Cardona

{"title":"Highly pathogenic avian influenza (HPAI H5N1, clade 2.3.4.4b, genotype C2.1) in commercial ring-necked pheasants during the 2022 outbreak in the United States.","authors":"Kayla R Niel, Kaitlyn M St Charles, Jonathan Y Elissa, Megan E Lighty, Amos Ssematimba, Marie Culhane, Alvin Crespo-Bellido, Nídia S Trovão, Martha I Nelson, Carol Cardona","doi":"10.1139/cjm-2025-0198","DOIUrl":"10.1139/cjm-2025-0198","url":null,"abstract":"Highly pathogenic avian influenza (HPAI) virus H5N1 clade 2.3.4.4b was introduced into North America in 2021. In 2022, clade 2.3.4.4b spilled into domestic poultry in the United States (U.S.), resulting in the largest HPAI outbreak in U.S. history. In December 2023, H5N1 2.3.4.4b was detected on an upland game bird farm in Pennsylvania. History, clinical signs, and gross lesions were suggestive of marble spleen disease. However, HPAI was identified by real-time reverse-transcriptase polymerase chain reaction; gene sequencing confirmed genotype C2.1 with phylodynamic analyses providing evidence of a wild bird introduction. Predictive mathematical modeling estimated the time of virus introduction onto the farm (15 days (95% C.I., 11-23) before the date of confirmed detection) being similar or longer than previously described for domestic poultry. Although bioexclusion measures were unable to prevent the initial exposure due to industry rearing practices, biocontainment procedures appeared to prevent spread. The lessons learned from this case may be important for other animal agriculture commodities, especially those species that are infrequently handled or observed and may have inaccurately calculated mortality levels. This well-characterized outbreak and other experiences with natural infection should be taken as opportunities to better understand the field dynamics of this virus.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-17"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal activity of emodin against the Cryptococcus neoformans/Cryptococcus gattii complexes: an effect on the enzyme superoxide dismutase. 大黄素对新型隐球菌/加蒂隐球菌复合物的抗真菌活性：对酶超氧化物歧化酶的影响。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0257

Paulo Ricardo Monteiro Araújo, Marrie da Silva Dutra, Maria Gleiciane Rocha, Vinícius Carvalho Pereira, Raissa Geovanna Pereira Lopes, Andreza Pereira Braga, Lorena Mayana Beserra de Oliveira, Pedro Filho Noronha Souza, Waldemiro Aquino Pereira-Neto, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Glaucia Morgana de Melo Guedes, Raquel Carvalho Montenegro, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha

{"title":"Antifungal activity of emodin against the Cryptococcus neoformans/Cryptococcus gattii complexes: an effect on the enzyme superoxide dismutase.","authors":"Paulo Ricardo Monteiro Araújo, Marrie da Silva Dutra, Maria Gleiciane Rocha, Vinícius Carvalho Pereira, Raissa Geovanna Pereira Lopes, Andreza Pereira Braga, Lorena Mayana Beserra de Oliveira, Pedro Filho Noronha Souza, Waldemiro Aquino Pereira-Neto, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Glaucia Morgana de Melo Guedes, Raquel Carvalho Montenegro, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha","doi":"10.1139/cjm-2025-0257","DOIUrl":"https://doi.org/10.1139/cjm-2025-0257","url":null,"abstract":"The Cryptococcus neoformans/Cryptococcus gattii species complexes include encapsulated yeasts that cause cryptococcosis. Emodin has demonstrated antimicrobial activity. This study evaluated emodin's antifungal activity against both planktonic cells and mature biofilms of the Cryptococcus neoformans/Cryptococcus gattii complexes, and its interaction with superoxide dismutase (SOD). Planktonic susceptibility was assessed by broth microdilution (CLSI; M27-A4). Mature biofilms were treated with emodin (128-1024 µg mL-1) and evaluated by crystal violet staining for biomass and the [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium] (MTT) assay for metabolic activity. Confocal laser microscopy (CLSM) and LIVE/DEAD™ staining were used to analyze viability and structure. Molecular docking assays with AutoDock Vina showed emodin's potential to bind SOD, and this was validated through the nitro blue tetrazolium (NBT) reduction assay. Emodin had inhibitory effects on planktonic cells, with MIC values ranging from 256 to 512 µg mL-1. Against mature biofilms, emodin at 1024 µg mL-1 reduced biomass by 72% and metabolic activity by 99% compared to the drug-free control. Additionally, emodin reduced structural parameters of biofilms and SOD activity, supporting its role in oxidative stress induction. These findings highlight emodin's potential antifungal activity against Cryptococcus neoformans/Cryptococcus gattii complexes biofilms and inhibitory effect on SOD activity as a potential mechanism of action.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"72 ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and antifungal properties against Botrytis cinerea of bacteria isolated from hemp seed oil. 大麻籽油分离细菌的鉴定及对灰霉病菌的抑菌性能。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0241

Mohannad Mahmoud, Philippe Seguin, Sébastien P Faucher, Suha Jabaji

{"title":"Characterization and antifungal properties against Botrytis cinerea of bacteria isolated from hemp seed oil.","authors":"Mohannad Mahmoud, Philippe Seguin, Sébastien P Faucher, Suha Jabaji","doi":"10.1139/cjm-2025-0241","DOIUrl":"10.1139/cjm-2025-0241","url":null,"abstract":"Botrytis cinerea is a pathogen infecting Cannabis sativa L. plants, causing economic losses, and can develop resistance to chemical fungicides, the use of which is restricted in cannabis production. Thus, developing biocontrol methods is imperative. Seven bacterial strains were isolated from hemp seed oil, characterized, and examined for the potential to control a Botrytis cinerea isolate from cannabis. Three isolates, Bacillus mojavensis HOB3, Paenibacillus sp. HOB6, and Bacillus subtilis HOB7 exhibited significant inhibition of Botrytis cinerea. These isolates were further evaluated for their biosurfactant activity using two liquid media, Lysogeny Broth (LB) and hydrocarbon-amended Bushnell and Haas (BH). The oil-spreading and drop-collapse assays revealed growth-medium-dependent variation in surface activity associated with biosurfactant presence. The BH cell-free extract (BH-CFE) of Bacillus subtilis HOB7 showed the highest estimated biosurfactant presence and antifungal activity against Botrytis cinerea, but both activities were absent when using the LB cell-free extract (LB-CFE) of Bacillus subtilis HOB7. Thus, a potential relationship between antifungal activity and biosurfactant production was suggested. Genome mining of the strains identified gene clusters encoding compounds with antifungal activity, including the biosurfactants polymyxin B, fusaricidin B, fengycin, and surfactin. To our knowledge, this is the first report of the isolation of hemp seed oil bacteria with potential biocontrol properties against fungal phytopathogens.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-15"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and impact of high pathogenicity avian influenza in Europe 2020-2025. 2020 - 2025年欧洲高致病性禽流感流行病学及影响

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0214

Ian H Brown, Chris Neil

{"title":"Epidemiology and impact of high pathogenicity avian influenza in Europe 2020-2025.","authors":"Ian H Brown, Chris Neil","doi":"10.1139/cjm-2025-0214","DOIUrl":"10.1139/cjm-2025-0214","url":null,"abstract":"High pathogenicity avian influenza (HPAI) of H5 subtype was detected year-round for the first time in Europe in 2022 and contributed to the largest European HPAI epizootic. The switch from H5N8 to H5N1 virus subtypes heralded a substantial increase in cases in wild and domestic birds. The impact on domestic poultry production was substantial with more than 120 million birds dying or being culled to control the disease. This was partly attributed to an expansion in the host range of wild birds affected, with infection of orders of birds not previously described as susceptible. The consequence of these transmission events into naive wild bird populations was often large-scale mortality, particularly in seabirds 2022-2023. Relatively high infection pressure provided opportunity for exposure and spillover to wild mammals principally of the order Carnivora, exposed through predatory or scavenging behaviour. Other sporadic infections involved farmed fur animals and a single detection in a sheep in England, but no evidence of infection in other livestock species including dairy cattle. Human infections were rare and asymptomatic, associated with very close contact with infected domestic birds. The epizootic was sustained by continuing evolution in the virus principally through genetic reassortment with selection and fixation of genotypes of high fitness in the avian population.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventive effects of synbiotic supplementation against Salmonella Enteritidis infection in broiler chickens. 复合制剂对肉鸡肠炎沙门氏菌感染的预防作用。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0283

Jaime Daniel Babot, Víctor Maximiliano Hidalgo, María Magdalena Fernández, Horacio Raúl Terzolo, Adriana Perez Chaia, María Cristina Apella

{"title":"Preventive effects of synbiotic supplementation against Salmonella Enteritidis infection in broiler chickens.","authors":"Jaime Daniel Babot, Víctor Maximiliano Hidalgo, María Magdalena Fernández, Horacio Raúl Terzolo, Adriana Perez Chaia, María Cristina Apella","doi":"10.1139/cjm-2025-0283","DOIUrl":"10.1139/cjm-2025-0283","url":null,"abstract":"The restriction of antibiotics as growth promoters in poultry has intensified the search for alternative strategies to control enteric pathogens. This study aimed to develop a multi-strain probiotic composed of Enterococcus faecium CRL 1385, Ligilactobacillus salivarius subsp. salivarius CRL 1417, and Lactobacillus crispatus CRL 1453, formulate a synbiotic by combining this mixture with raffinose and molasses, and evaluate their preventive efficacy against Salmonella infection in broiler chicks. Strain compatibility was assessed using a well-diffusion assay with cell-free supernatants, confirming absence of antagonism among strains. In vitro co-culture assays demonstrated a clear bactericidal effect, with inactivation of Salmonella Enteritidis PT4 1222 and Salmonella Gallinarum INTA 91/A1. Ex vivo assays using intestinal explants showed that pre-treatment with probiotic or synbiotic formulations significantly reduced Salmonella adhesion, suggesting that the primary protective mechanism involved inhibition of pathogen attachment to epithelial surfaces. For in vivo evaluation, chicks received daily treatments from day 1 post-hatch and were challenged via crop injection on day 3. Synbiotic administration significantly reduced S. Enteritidis infection rates (30% vs. 100% in controls), while all treatments failed to reduce S. Gallinarum prevalence. These findings highlight the potential of early-life synbiotic administration as a sustainable strategy to prevent S. Enteritidis infections in poultry.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pangenome-GWAS analysis of Clostridioides difficile resistant traits from healthcare- and non-healthcare-associated settings. 医疗保健和非医疗保健相关环境中艰难梭菌抗性性状的泛基因组- gwas分析

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0288

Manuel Jara, Alba Frias-De-Diego, Robert A Petit, Loc Nguyen, Cristina Lanzas

{"title":"Pangenome-GWAS analysis of Clostridioides difficile resistant traits from healthcare- and non-healthcare-associated settings.","authors":"Manuel Jara, Alba Frias-De-Diego, Robert A Petit, Loc Nguyen, Cristina Lanzas","doi":"10.1139/cjm-2025-0288","DOIUrl":"10.1139/cjm-2025-0288","url":null,"abstract":"Clostridioides difficile (C. difficile) is a gastrointestinal pathogen that causes healthcare-associated infections with numerous mechanisms to acquire and confer antimicrobial resistance (AMR) and rapidly adapt to novel environments. These environments extend beyond healthcare, and encompass non-healthcare-related settings, where antimicrobials engender distinct selection pressures. Employing a dataset of 3870 whole-genome sequences of C. difficile from healthcare and non-healthcare environments, this study investigates the selection pressures associated with each setting and its impact on the prevalence of AMR genes of C. difficile. To achieve this, we used pangenome-wide association studies (pan-GWAS) and generalized linear mixed model to identify significant associations between AMR genes and the specific setting from which the isolates were collected. Our findings evidenced significant differences in the AMR gene repertoire between healthcare- and non-healthcare-associated C. difficile with antibiotics such as macrolides and fluoroquinolones being more linked with healthcare. Antibiotics commonly used in agriculture such as aminoglycosides or tetracyclines also presented associations with non-healthcare environments. Our work offers a comprehensive exploration of C. difficile AMR determinants across healthcare- and non-healthcare-related settings shedding light on the selection pressures present in each environment, enhancing our understanding of C. difficile resistome and its influence on the bacterium's opportunistic colonization nature.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel evolutionary trajectories of extensively drug-resistant Klebsiella pneumoniae ST258 and ST307 pandemic clones: a systematic review. 广泛耐药肺炎克雷伯菌ST258和ST307大流行克隆的平行进化轨迹：系统综述

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2026-01-01 DOI: 10.1139/cjm-2025-0268

Sampurna Nivedita Sarkar, Surojit Das

{"title":"Parallel evolutionary trajectories of extensively drug-resistant Klebsiella pneumoniae ST258 and ST307 pandemic clones: a systematic review.","authors":"Sampurna Nivedita Sarkar, Surojit Das","doi":"10.1139/cjm-2025-0268","DOIUrl":"10.1139/cjm-2025-0268","url":null,"abstract":"Extensively drug-resistant (XDR) Klebsiella pneumoniae poses a critical threat to global public health. ST258 and ST307 are two pandemic clones that have emerged through distinct evolutionary mechanisms. Understanding their parallel trajectories is essential for clinical management and therapeutic development. This systematic review examined peer-reviewed literature on K. pneumoniae ST258 and ST307 (1995-2025) from PubMed, Scopus, Google Scholar, and Web of Science. We extracted data on resistance genes, virulence factors, geographic distribution, and clinical outcomes. Inclusion criteria: studies with molecular or genomic characterization of ST258/ST307 isolates. Analysis revealed distinct evolutionary pathways. ST258 (emerged 1995, USA) showed recombination driven evolution with stable blaKPC-2/3 resistance, remaining hospital-endemic and declining after 2015. ST307 (emerged 2008, Netherlands) demonstrated plasmid-mediated adaptability with diverse carbapenemases (blaNDM, blaOXA-48, blaKPC), achieving rapid global dissemination across six WHO regions by 2025. Both achieved pandemic status through different mechanisms. ST307 shows emerging hypervirulent sublineages in Europe and South America. Two distinct evolutionary strategies converge to create a unified pandemic threat. ST258's stability contrasts with ST307's adaptability, reflecting different fitness landscapes. Both clones exhibit limited susceptibility to current therapeutic options, with emerging resistance to last-resort agents. ST258 and ST307 represent complementary pandemic threats requiring coordinated global surveillance, antimicrobial development, and research into evolutionary dynamics.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between common scab-inducing strains on potato suberin. 马铃薯单宁上常见疮痂诱导菌株之间的相互作用。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0242

Mario Khalil, Iauhenia Isayenka, Rebeca Padilla-Reynaud, Sylvain Lerat, Carole Beaulieu

{"title":"Interactions between common scab-inducing strains on potato suberin.","authors":"Mario Khalil, Iauhenia Isayenka, Rebeca Padilla-Reynaud, Sylvain Lerat, Carole Beaulieu","doi":"10.1139/cjm-2024-0242","DOIUrl":"10.1139/cjm-2024-0242","url":null,"abstract":"Potato tuber periderm is armored with suberin, that consists of two domains, an aliphatic domain composed of fatty acid polyesters and an aromatic domain composed of cinnamic acids. Streptomyces scabies 87.22, a predominant causal agent of potato common scab, was compared for adaptation to tuber suberin with Streptomyces acidiscabies ATCC 49003 and Streptomyces turgidiscabies Car8 belonging to emerging pathogenic species. Streptomyces scabies 87.22 showed higher growth in the suberin supplemented medium than the two other strains. When co-cultured in a rich nutrient medium, S. acidiscabies ATCC 49003 produced the antibiotic oxanthromicin, which inhibited growth and mycelium development of the other strains. Exposure of S. scabies 87.22 and S. acidiscabies ATCC 49003 to suberin was accompanied by the secretion of enzymes degrading cellulose, hemicellulose, fatty acids, and glycerol derivatives. Compared to the two other strains, S. scabies 87.22 showed higher esterase activity in suberin-supplemented medium and strong induction of cellulase gene expression. Both S. acidiscabies ATCC 49003 and S. turgidiscabies Car8 exhibited a poor utilization of trans-ferulic and p-coumaric acids, suggesting almost no ability to degrade the aromatic moiety of suberin. This work suggests that S. scabies 87.22 is better adapted to the potato periderm degradation than the emerging pathogens. The elucidation of pathogenic Streptomyces strains interaction may contribute to the improvement of ecologically oriented agronomic strategies for common scab management.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-15"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0