B Van der Merwe, J Williams, E Le Roux, A Van Staden, G Lawrence, K Jacobs
{"title":"<i>Morchella capensis</i> sp. nov., the Fynbos morel-the first description of an endemic <i>Morchella</i> species from Africa.","authors":"B Van der Merwe, J Williams, E Le Roux, A Van Staden, G Lawrence, K Jacobs","doi":"10.1139/cjm-2023-0224","DOIUrl":"https://doi.org/10.1139/cjm-2023-0224","url":null,"abstract":"<p><p>A species of <i>Morchella</i> was observed growing in spring, under a vulnerable member of the <i>Proteaceae</i>, in the Cape Floristic Region of South Africa. These fungi shared many of the cryptic characteristics common in the genus <i>Morchella</i> and displayed a wide range of phenotypic expression. The unique ecology of these fungi and the fact that no endemic <i>Morchella</i> species have been described from Africa lead to suspicions that this could be a novel species. Sequencing of key genetic regions, phylogenetics, and morphological studies confirmed that this was indeed a previously unknown species of <i>Morchella</i>. Roots collected underneath the fruiting bodies displayed a range of root-associated activities, alluding to a possible relationship. Further, this <i>Morchella</i> species has a history of traditional use on the Cape Peninsula of South Africa. The traditional use of fungi is rarely recorded in Africa. In this study, we introduce <b><i>Morchella capensis</i> sp. nov.</b>, the first endemic African morel.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"71 ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barney A Geddes, Riley Williamson, Jake Schumacher, Ahmad Ardi, Garrett Levin, Emily Červenka, Rui Huang, George C diCenzo
{"title":"BEVA2.0: modular assembly of golden gate-compatible vectors with expanded utility for genetic engineering.","authors":"Barney A Geddes, Riley Williamson, Jake Schumacher, Ahmad Ardi, Garrett Levin, Emily Červenka, Rui Huang, George C diCenzo","doi":"10.1139/cjm-2024-0246","DOIUrl":"10.1139/cjm-2024-0246","url":null,"abstract":"<p><p>This expansion for the modular vector assembly platform BEVA (Bacterial Expression Vector Archive) introduces 11 new BEVA parts including two new cloning site variants, two new antibiotic resistance modules, three new origins of replication, and four new accessary modules. As a result, the modular system is now doubled in size and expanded in its capacity to produce diverse replicating plasmids. Furthermore, it is now amenable to genetic engineering methods involving genome-manipulation of target strains through deletions or integrations. In addition to introducing the new modules, we provide several BEVA-derived Golden Gate cloning plasmids that are used to validate parts and that may be useful for genetic engineering of proteobacteria and other bacteria. We also introduce new parts to allow compatibility with the CIDAR MoClo parts libraries.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoyun Qi, Suhui Hou, Wenge Hu, Cheng Ding, Yang Li, Jie Xiong
{"title":"Diversity and distribution characteristics of myxobacteria in the rhizosphere and nonrhizosphere soils of the halophyte <i>Haloxylon ammodendron</i> in the high saline-alkaline Ebinur Lake Wetland.","authors":"Xiaoyun Qi, Suhui Hou, Wenge Hu, Cheng Ding, Yang Li, Jie Xiong","doi":"10.1139/cjm-2024-0045","DOIUrl":"https://doi.org/10.1139/cjm-2024-0045","url":null,"abstract":"<p><p>This study employed Illumina HiSeq high-throughput sequencing technology to analyze the V4-V5 regions of myxobacterial 16S rRNA in rhizosphere and nonrhizosphere soils of <i>Haloxylon ammodendron</i> in the saline-alkaline wetland of Ebinur Lake, with the aim of investigating the community structure and diversity of myxobacteria. Results indicated that myxobacterial communities in rhizosphere soils exhibited greater diversity and richness compared to nonrhizosphere soils. Soil physicochemical properties, particularly moisture content, were identified as key environmental factors influencing myxobacterial diversity. The halotolerant genus <i>Haliangium</i> was found to be predominant under saline-alkaline conditions. Additionally, myxobacteria demonstrated distinct ecological specificity and environmental adaptability between rhizosphere and nonrhizosphere soils. For example, the genus <i>Enhygromyxa</i> exhibited a negative correlation with soil moisture content in rhizosphere soils but a positive correlation with soil electrical conductivity in nonrhizosphere soils. Co-occurrence network analysis revealed complex interaction patterns among myxobacterial genera and other bacterial genera, with closer interactions observed in rhizosphere soils. This study highlights the importance of environmental factors in regulating microbial community structure and function in saline-alkaline wetlands, providing new insights into the ecological roles and interaction mechanisms of myxobacteria within the ecosystem.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"71 ","pages":"1-17"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hannah G Braun, Sumudu R Perera, Yannick Dn Tremblay, Jenny-Lee Thomassin
{"title":"Antimicrobial resistance in <i>Klebsiella pneumoniae</i>: an overview of common mechanisms and a current Canadian perspective.","authors":"Hannah G Braun, Sumudu R Perera, Yannick Dn Tremblay, Jenny-Lee Thomassin","doi":"10.1139/cjm-2024-0032","DOIUrl":"10.1139/cjm-2024-0032","url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> is a ubiquitous opportunistic pathogen of the family Enterobacteriaceae. <i>K. pneumoniae</i> is a member of the ESKAPEE pathogens (<i>Enterococcus faecium, Staphylococcus aureus, K. pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter</i> spp., and <i>Escherichia coli</i>), a group of bacteria that cause nosocomial infections and are able to resist killing by commonly relied upon antimicrobial agents. The acquisition of antimicrobial resistance (AMR) genes is increasing among community and clinical isolates of <i>K. pneumoniae,</i> making <i>K. pneumoniae</i> a rising threat to human health. In addition to the increase in AMR, <i>K. pneumoniae</i> is also thought to disseminate AMR genes to other bacterial species. In this review, the known mechanisms of <i>K. pneumoniae</i> AMR will be described and the current state of AMR <i>K. pneumoniae</i> within Canada will be discussed, including the impact of the coronavirus disease-2019 pandemic, current perspectives, and outlook for the future.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"507-528"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jakkrapong Kangsopa, Russell K Hynes, Boonmee Siri
{"title":"Lettuce seed pelleting with <i>Pseudomonas</i> sp. 31-12: plant growth promotion under laboratory and greenhouse conditions.","authors":"Jakkrapong Kangsopa, Russell K Hynes, Boonmee Siri","doi":"10.1139/cjm-2024-0071","DOIUrl":"10.1139/cjm-2024-0071","url":null,"abstract":"<p><p>Plant growth promotion by <i>Pseudomonas</i> sp. 31-12 incorporated into a lettuce seed pelleting matrix was studied. We examined (1) the effect of five rhizosphere derived bacterial strains on green oak lettuce (<i>Lactuca sativa</i> L.) seed germination, root and shoot growth, as a strain selection step for seed coating and seed pelletizing studies, (2) population stability of <i>Pseudomonas</i> sp. 31-12 incorporated into a pelleting matrix on lettuce seed stored three months at 4 °C, and (3) lettuce growth promotion in the laboratory and greenhouse by <i>Pseudomonas</i> sp. 31-12 coated and pelletized seed. A spontaneous streptomycin mutant of <i>Pseudomonas</i> sp. 31-12 (str) was used to determine population size on seed and roots of 15- and 30-day-old lettuce. The population of <i>Pseudomonas</i> sp. 31-12str on coated and pelleted seed decreased from 10<sup>4</sup> cfu/seed to 10<sup>3</sup> cfu/seed after 3 months storage at 4 °C. However, the population exceeded 10<sup>4</sup> cfu/g root dry mass and 10<sup>5</sup>/g root dry mass after 15 days and 30 days in the greenhouse. Leaf fresh mass was significantly increased (<i>P</i> ≤ 0.05) with <i>Pseudomonas</i> sp. 31-12 seed treatment as compared to noninoculated seed. In conclusion, pelletized lettuce seed with <i>Pseudomonas</i> sp. 31-12 promoted growth and yield in the greenhouse.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"529-537"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tracking the trajectory of frankia research through bibliometrics: trends and future directions.","authors":"Ridha Mhamdi, Maher Gtari","doi":"10.1139/cjm-2024-0030","DOIUrl":"10.1139/cjm-2024-0030","url":null,"abstract":"<p><p>Frankia represent a unique group of filamentous, sporangia-forming bacteria, renowned for their exceptional capacity to establish symbiotic partnerships with actinorhizal plants. The objective of this paper is to offer quantitative insights into the current state of frankia research and its future potential. A comprehensive bibliometric analysis covering the years 2000-2022 was conducted using Scopus and SciVal. A steady increase in both annual publication and international collaboration has been observed, particularly since 2013. Research performance metrics for the last 5 years (2018-2022) indicate China and India as leaders with high Field-Weighted Citation Impact scores. This analysis highlighted prominent authors, research groups, and the evolving research landscape, suggesting an increasing focus on molecular and genomic aspects. The genomic era has transformed our understanding of frankia biology, highlighting their significance in diverse ecological and agricultural contexts. This study comprehensively maps the evolving landscape of frankia research, emphasizing key milestones that have catalysed international interest in frankia-actinorhizal research, expanding our perception of frankia's capabilities beyond its traditional symbiotic role. As research in this field progresses, a deeper comprehension of frankia-plant interactions, symbiotic signalling, and the intricacies of metabolic pathways holds the promise of revealing innovative techniques for optimizing nitrogen fixation and broadening the spectrum of host plants.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"551-564"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Niokhor Bakhoum, Ethan Humm, Noor Khan, Ann M Hirsch
{"title":"Soil microbiomes from the groundnut basin of Senegal contain plant growth-promoting bacteria with potential for crop improvement in arid soils.","authors":"Niokhor Bakhoum, Ethan Humm, Noor Khan, Ann M Hirsch","doi":"10.1139/cjm-2024-0031","DOIUrl":"10.1139/cjm-2024-0031","url":null,"abstract":"<p><p>The principal methods to maintain soil fertility in Sahel soils are largely allowing fields to go fallow and manure addition. These methods are not currently sufficient to improve soil fertility. To promote biological amendments, we aimed to understand the plant-growth promoting traits of various soil microbial isolates. The soils collected in different areas in Senegal exhibited a similar eDNA profile of bacteria; the dominant microbes were Firmicutes, followed by Proteobacteria and Actinobacteria. Of 17 isolates identified and tested, the vast majority solubilized rock phosphate and a large number grew on culture medium containing 6% salt, but very few degraded starches or hydrolysed carboxymethyl cellulose or produced siderophores. Upon single inoculation, <i>Peribacillus asahii</i> RC16 and <i>Dietzia cinnamea</i> 55 significantly increased pearl millet growth and yield parameters. For cowpea, plant shoot length was significantly increased by <i>Pseudarthrobacter phenanthrenivorans</i> MKAG7 co-inoculated with <i>Bradyrhizobium elkanii</i> 20TpCR5, and nearly all rhizobacteria tested significantly improved cowpea dry weight and pod weight. Additionally, the double inoculation of <i>Dietzia cinnamea</i> 55 and MKAG7 significantly increased shoot length, dry weight, and seed head weight of pearl millet. These isolates are promising inoculants because they are ecologically-friendly, cost-effective, sustainable, and have fewer negative effects on the soil and its inhabitants.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"538-550"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The roles of rabies virus structural proteins in immune evasion and implications for vaccine development.","authors":"Pei-Hua Wang, Li Xing","doi":"10.1139/cjm-2024-0023","DOIUrl":"10.1139/cjm-2024-0023","url":null,"abstract":"<p><p>Rabies is a zoonotic infectious disease that targets the nervous system of human and animals and has about 100% fatality rate without treatment. Rabies virus is a bullet-like viral particle composed of five structural proteins, including nucleoprotein (N), phosphorylated protein (P), matrix protein (M), glycoprotein (G), and large subunit (L) of RNA-dependent RNA polymerase. These multifunctional viral proteins also play critical roles in the immune escape by inhibiting specific immune responses in the host, resulting in massive replication of the virus in the nervous system and abnormal behaviors of patients such as brain dysfunction and hydrophobia, which ultimately lead to the death of patients. Herein, the role of five structural proteins of rabies virus in the viral replication and immune escape and its implication for the development of vaccines were systemically reviewed, so as to shed light on the understanding of pathogenic mechanism of rabies virus.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"461-469"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renee H Petipas, Cassidy Peru, Janice M Parks, Maren L Friesen, Chandra N Jack
{"title":"Prairie soil improves wheat establishment and accelerates the developmental transition to flowering compared to agricultural soils.","authors":"Renee H Petipas, Cassidy Peru, Janice M Parks, Maren L Friesen, Chandra N Jack","doi":"10.1139/cjm-2023-0237","DOIUrl":"10.1139/cjm-2023-0237","url":null,"abstract":"<p><p>Less than 1% of native prairie lands remain in the United States. Located in eastern Washington, the rare habitat called Palouse prairie was largely converted to wheat monocropping. With this conversion came numerous physical, chemical, and biological changes to the soil that may ultimately contribute to reduced wheat yields. Here, we explored how wheat (<i>Tritcum aestivum</i> L.) seedling establishment, plant size, and heading, signifying the developmental transition to flowering, were affected by being planted in prairie soil versus agricultural soils. We then sought to understand whether the observed effects were the result of changes to the soil microbiota due to agricultural intensification. We found that prairie soil enhanced both the probability of wheat seedling survival and heading compared to agricultural soil; however, wheat growth was largely unaffected by soil source. We did not detect effects on wheat developmental transitions or phenotype when inoculated with prairie microbes compared with agricultural microbes, but we did observe general antagonistic effects of microbes on plant size, regardless of soil source. This work indicates that agricultural intensification has affected soils in a way that changes early seedling establishment and the timing of heading for wheat, but these effects may not be caused by microbes, and instead may be caused by soil nutrient conditions.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"482-491"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Farhan Yusuf, Saher M Ahmed, Danica Dy, Katherine Baney, Hassan Waseem, Kimberley A Gilbride
{"title":"Occurrence and characterization of plasmid-encoded <i>qnr</i> genes in quinolone-resistant bacteria across diverse aquatic environments in southern Ontario.","authors":"Farhan Yusuf, Saher M Ahmed, Danica Dy, Katherine Baney, Hassan Waseem, Kimberley A Gilbride","doi":"10.1139/cjm-2024-0029","DOIUrl":"10.1139/cjm-2024-0029","url":null,"abstract":"<p><p>Antimicrobial resistance is an ever-increasing threat. The widespread usage of ciprofloxacin has led to the manifestation of resistance due to chromosomal mutations or the acquisition of plasmid-mediated quinolone resistance (PMQR) traits. Some particular PMQR traits, <i>qnr</i> genes, have been identified globally in clinical and environmental isolates. This study aimed to determine the prevalence of ciprofloxacin-resistant bacteria in aquatic environments in southern Ontario and investigate the extent of dissemination of ciprofloxacin resistance traits among the bacterial communities. We surveyed the prevalence of plasmid encoding <i>qnr</i> genes using a multiplex PCR assay of associated PMQR genes, <i>qnr</i>A, <i>qnr</i>B, and <i>qn</i>rS, on 202 isolates. Despite the absence of significant impacts on minimum inhibitory concentration levels, the presence of <i>qnr</i> genes correlates with heightened resistance to quinolones and nalidixic acid in some isolates. Taxonomic analysis highlights distinct differences in the composition and diversity of ciprofloxacin-sensitive (CipS) and ciprofloxacin-resistant (CipR) populations, with Proteobacteria dominating both groups. Importantly, CipR populations exhibit lower genetic diversity but higher prevalence of multiple antibiotic resistances, suggesting co-selection mechanisms. Co-occurrence analysis highlights significant associations between ciprofloxacin resistance and other antibiotic resistances, implicating complex genetic linkages. The results of our study signified the critical role of environmental monitoring in public health.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"492-506"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}