Comparative mitogenomics of Leptographium procerum, Leptographium terebrantis and Leptographium wingfieldii, an invasive fungal species in Canadian forests.

Abstract

Leptographium wingfieldii is a fungal associate of Tomicus piniperda (the pine shoot beetle) and pathogen of pines and this species is an agent of blue stain in sapwood on infected trees. This fungus was first reported from Europe and has been recently introduced to Canadian forests. Ten new mitogenomes have been sequenced and characterized, including seven strains of L. wingfieldii, two strains of L. procerum and one strain of L. terebrantis. The data were combined with other members of the Ophiostomatales collected from NCBI to gain more insight into the genetic diversity, evolution, and systematics of these fungi. The size of the studied mitogenomes of Leptographium species ranged from 41 kb to 126 kb with the number of potential mobile introns embedded within these mitogenomes ranging from 13 to 45. These data show that introns generate genetic diversity and confirms the contribution of mobile introns in genome expansion in Ophiostomatales fungi. This study also uncovered complex intron arrangements (twintrons) suggesting the potential of mobile introns generating complex ribozymes that may have implications in gene regulation.