Surveillance of Clostridioides difficile in Canadian retail meat and genomic linkages to community-associated human clinical infections in Canada.

IF 1.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Paula E Pidsadny, Tim Du, Romeo Hizon, Sean Ahmed, Derek Tan, George G Zhanel, Denice C Bay, Richard J Reid-Smith, Audrey Charlebois, George R Golding
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引用次数: 0

Abstract

Community-associated Clostridioides difficile infections (CA-CDI) remain a concern in Canada, comprising a quarter of cases previously reported through the Canadian Nosocomial Infection Surveillance Program. Previous Canadian studies have reported toxigenic C. difficile isolated from Canadian retail meat, suggesting that it may be a source of exposure for CA-CDI in Canada. In this study, 3/219 (1.4%) of retail pork and 0/99 (0%) of retail beef samples tested positive for toxigenic C. difficile, which were molecularly characterized by PCR ribotyping and whole-genome sequencing. All three isolates were obtained from pork and belonged to sequence types (STs)/ribotypes (RTs) that have previously been isolated from human clinical CA-CDI cases in Canada: ST1/RT027, ST8/RT002, and ST10/RT015. Retail meat isolates were susceptible to the antimicrobials tested, save one isolate with intermediate resistance to clindamycin. Genomic comparison to Canadian human clinical CA-CDI isolates with the same corresponding ST/RT types showed two of the three pork isolates clustered with CA-CDI isolates via core-genome multilocus sequencing typing, with single nucleotide variant (SNV) analysis showing further genomic relatedness of 2-11 SNVs. Retail meat may therefore be a low source of CA-CDI exposure in Canada, with the potential for foodborne transmission of select clones.

加拿大零售肉类中艰难梭菌的监测以及与加拿大社区相关人类临床感染的基因组联系。
社区相关性艰难梭菌感染(CA-CDI)在加拿大仍是一个令人担忧的问题,占加拿大医院感染监测项目先前报告病例的四分之一。加拿大先前的研究报告了从加拿大零售肉类中分离出的产毒艰难梭菌,这表明它可能是加拿大CA-CDI的暴露源。在本研究中,3/219(1.4%)的零售猪肉和0/99(0%)的零售牛肉样品检测出产毒艰难梭菌阳性,并通过PCR核糖分型和全基因组测序对其进行了分子表征。这三株分离株均来自猪肉,均属于先前从加拿大临床CA-CDI病例中分离到的序列型(ST)/核糖型(RT): ST1/RT027、ST8/RT002和ST10/RT015。零售肉类分离株对所测试的抗菌素敏感,除一株对克林霉素具有中等耐药性外。与具有相同ST/RT型的加拿大人临床CA-CDI分离株的基因组比较显示,通过核心基因组多位点测序分型,3株猪肉分离株中有2株与CA-CDI分离株聚类,单核苷酸变异(SNV)分析显示2株SNV进一步具有基因组亲缘性。因此,零售肉类可能是加拿大CA-CDI暴露的低来源,具有选择克隆的食源性传播的潜力。
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来源期刊
CiteScore
4.80
自引率
0.00%
发文量
71
审稿时长
2.5 months
期刊介绍: Published since 1954, the Canadian Journal of Microbiology is a monthly journal that contains new research in the field of microbiology, including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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