Canadian journal of microbiology最新文献_第3页

A β-amino-ketone that disrupts the fungal plasma membrane exhibits potent activity against pathogenic Trichophyton species. 一种破坏真菌质膜的β-氨基酮显示出对致病性毛癣菌的有效活性。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2025-0009

Jéssica Cristina Polveiro Campos, Diego Batista Carneiro de Oliveira, Bruna da Silva Souza, Lucas Felipe de Oliveira, Ezequias Pessoa de Siqueira, Markus Kohlhoff, Anderson Assunção Andrade

{"title":"A β-amino-ketone that disrupts the fungal plasma membrane exhibits potent activity against pathogenic Trichophyton species.","authors":"Jéssica Cristina Polveiro Campos, Diego Batista Carneiro de Oliveira, Bruna da Silva Souza, Lucas Felipe de Oliveira, Ezequias Pessoa de Siqueira, Markus Kohlhoff, Anderson Assunção Andrade","doi":"10.1139/cjm-2025-0009","DOIUrl":"10.1139/cjm-2025-0009","url":null,"abstract":"Superficial fungal infections, mainly caused by dermatophytes, are a global public health issue. We evaluated the antifungal activity of six β-amino-ketones against Trichophyton rubrum, a leading agent of superficial mycoses. Among them, 3-(morpholin-4-yl)-1-phenylpropan-1-one (AB1) showed the most potent effect, with a minimum inhibitory concentration (MIC) of 7.81 µg/mL against the T. rubrum reference strain and fungicidal activity against clinical isolates, as demonstrated by minimum fungicidal concentration assays. AB1 was effective against both conidia and hyphae of T. rubrum, while showing limited activity against Candida albicans and the bacteria Escherichia coli and Staphylococcus aureus. Mechanistic studies suggest AB1 targets the fungal plasma membrane, possibly via ergosterol interactions, supported by increased MICs in ergosterol-rich conditions and membrane integrity assays. Confocal microscopy revealed morphological alterations in AB1-treated hyphae, indicative of membrane damage. Transmission electron microscopy confirmed cytoplasmic disorganization and membrane disruption at subinhibitory concentrations. Toxicological assays showed moderate cytotoxicity in human fibroblasts (IC50 = 37.75 µg/mL) and no toxicity in Galleria mellonella larvae at high doses. These findings highlight AB1 as a promising antifungal candidate against Trichophyton spp., with the potential benefit of reduced impact on the host microbiota compared to broad-spectrum antimicrobials.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Requirements and considerations for effective implementation of integrated One Health antimicrobial resistance research. 有效实施“一体卫生”抗菌素耐药性综合研究的要求与考虑

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0194

Dominic Poulin-Laprade, Jordyn Broadbent, Damien Biot-Pelletier, Susanne Kraemer, Emma J Griffiths, Ayush Kumar, Xian-Zhi Li, Catherine D Carrillo, Rahat Zaheer, Tim A McAllister, Sigrun Kullik, Ernesto Liebana, Nicole Ricker, Alexandra Langlois, Richard J Reid-Smith, Sébastien P Faucher, Gabriela Flores-Vargas, Emilie Bédard, J Kimberley Summers, Veronica Jarocki, Thanaporn Thongthum, Carolee A Carson

{"title":"Requirements and considerations for effective implementation of integrated One Health antimicrobial resistance research.","authors":"Dominic Poulin-Laprade, Jordyn Broadbent, Damien Biot-Pelletier, Susanne Kraemer, Emma J Griffiths, Ayush Kumar, Xian-Zhi Li, Catherine D Carrillo, Rahat Zaheer, Tim A McAllister, Sigrun Kullik, Ernesto Liebana, Nicole Ricker, Alexandra Langlois, Richard J Reid-Smith, Sébastien P Faucher, Gabriela Flores-Vargas, Emilie Bédard, J Kimberley Summers, Veronica Jarocki, Thanaporn Thongthum, Carolee A Carson","doi":"10.1139/cjm-2024-0194","DOIUrl":"10.1139/cjm-2024-0194","url":null,"abstract":"The One Health (OH) approach recognizes the interconnectedness of the health of people, animals, plants/crops and ecosystems, and is central to addressing antimicrobial resistance (AMR). The 7th Environmental Dimension of Antimicrobial Resistance Conference (EDAR7), held in Montreal in May 2024, exemplified this approach by convening international experts and stakeholders to discuss AMR research and policy progress. EDAR7 workshop #8 focused on (1) barriers to establishing effective OH AMR research programs, (2) gaps in OH AMR research priorities, and (3) potential solutions/approaches or \"tools\" to ensure programs develop in accordance with OH principles and generate insightful data that maximizes limited resources. Key workshop outcomes included identifying critical principles for OH AMR research programs and highlighting the pivotal role of sustainable data management strategies. Additionally, the importance of considering AMR policy and risk assessment needs when planning and designing research was emphasized. Discussions explored specific tools and approaches that support the standardized and harmonized collection and analysis of data, and associated challenges of integrating genomics data into current risk assessments and models. Synthesis of the workshop's discussions outlined critical considerations that interdisciplinary OH AMR research programs and networks should prioritize to enhance the impact of their outputs.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-17"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

11th International Symposium on Avian Influenza. 第11届禽流感国际研讨会。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2025-0196

Andrew S Lang, Michelle Wille

引用次数: 0

Microbial biodiversity of agricultural surface waters in Ontario, Canada. 加拿大安大略省农业地表水微生物多样性。

IF 1.6 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2025-0015

Izhar U H Khan, Yutao Wang, Tiequan Zhang, Keri Malanchuk, Shanwei Xu, Sophie Crevecoeur, Josh Wang, Arthur Zastepa, Xiaoji Liu

{"title":"Microbial biodiversity of agricultural surface waters in Ontario, Canada.","authors":"Izhar U H Khan, Yutao Wang, Tiequan Zhang, Keri Malanchuk, Shanwei Xu, Sophie Crevecoeur, Josh Wang, Arthur Zastepa, Xiaoji Liu","doi":"10.1139/cjm-2025-0015","DOIUrl":"https://doi.org/10.1139/cjm-2025-0015","url":null,"abstract":"Understanding the microbial diversity is the first step to monitor the microbial quality of agricultural surface waters. Here we report a study comparing the microbial composition and diversities in agricultural surface waters in Ontario, Canada, including the streams adjacent to dairy cattle production in eastern Ontario and the field runoffs from the manure-impacted experimental farm of south-western Ontario. We also studied the quality of the surface water in the western basin of Lake Erie during a harmful algal bloom (cHAB). In eastern Ontario, we found significantly lower (P < 0.01) abundance of Proteobacteria and Burkholderiales in the streams adjacent to dairy production than the non-agriculture site. Bacteroidetes was more abundant (P < 0.0001) in medium/high order streams and agriculture/urban mixed site. Seasonal peaks of Flavobacterium were observed in low order streams but the trend was not in medium/high order streams. In south-western Ontario, the dominant bacterial taxa in field runoffs was Pseudomonas, regardless of manure types. We also discovered that the elevated total dissolved nitrogen, total dissolved phosphorus and chlorophyll a associated with the cHAB zone in Lake Erie was further away rather from the agricultural sites. In conclusion, our studies identified unique microbiome patterns associated with agricultural surface waters in Ontario.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"71 ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of fungal endophytes in barley under Fusarium head blight infection. 大麦赤霉病侵染下真菌内生菌的调查。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0137

Denice Embrador, Zoe Quill, James R Tucker, Keval Shah, Ana Badea, Champa Wijekoon

{"title":"Survey of fungal endophytes in barley under Fusarium head blight infection.","authors":"Denice Embrador, Zoe Quill, James R Tucker, Keval Shah, Ana Badea, Champa Wijekoon","doi":"10.1139/cjm-2024-0137","DOIUrl":"10.1139/cjm-2024-0137","url":null,"abstract":"Fusarium head blight (FHB) is a devastating fungal disease caused by Fusarium graminearum, which affects barley (Hordeum vulgare L.) and other small cereal grains. Fungal endophytes are microorganisms that reside inside tissues and considered that they may have been involved in various roles of the plants. This study involved the comparison of fungal endophytes between \"non-infected/clean\" and \"FHB-infected\" barley genotypes in various tissues collected at different plant developmental stages and were grown under different conditions (i.e., greenhouse, research field, and FHB-field nursery). We hypothesized that fungal endophytes diversity and abundance may differ between plant tissues in various barley genotypes that were non-infected and FHB-infected. The 18S-internal transcribed spacer sequencing analysis revealed a greater number of fungal operational taxonomic units (OTUs) and endophyte species in FHB-infected barley compared to clean barley. A one-way ANOVA and Tukey's pairwise comparison test (p ≤ 0.05) were performed to test significant differences. Higher seed endophyte diversity was found in FHB-infected (120 OTUs) compared to non-infected (113 OTUs) harvested in 2021. The increase in diversity of endophytes that contributes to different roles in plant protection and defense, such as biocontrol agents, may prevent the growth of Fusarium species and decrease FHB-infection.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-16"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of cannabidiol (CBD), a cannabis plant derivative, against Candida albicans growth and biofilm formation. 大麻二酚（CBD）（一种大麻植物衍生物）对白色念珠菌生长和生物膜形成的影响。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-17 DOI: 10.1139/cjm-2024-0034

Maryam Bahraminia, Shujun Cui, Ze Zhang, Abdelhabib Semlali, Étienne Le Roux, Kelly-Anne Giroux, Camille Lajoie, François Béland, Mahmoud Rouabhia

引用次数: 0

Phenotypic and transcriptomic changes in Acinetobacter baumannii in rich and minimal growth mediums. 鲍曼不动杆菌在丰富和少量生长培养基中的表型和转录组学变化。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0175

Vanessa Kornelsen, Dawn White, Ayush Kumar

{"title":"Phenotypic and transcriptomic changes in Acinetobacter baumannii in rich and minimal growth mediums.","authors":"Vanessa Kornelsen, Dawn White, Ayush Kumar","doi":"10.1139/cjm-2024-0175","DOIUrl":"10.1139/cjm-2024-0175","url":null,"abstract":"Acinetobacter baumannii is an opportunistic pathogen that is often studied in commonly used rich media in laboratories worldwide. Due to the metabolic versatility of A. baumannii, it can be cultured in different growth mediums; however, this can lead to genotypic and phenotypic variations. In this study, we compared phenotypic and transcriptomic changes in A. baumannii ATCC17978-VU cultured in M9 minimal media supplemented with 20 mmol/L sodium succinate and rich lysogeny broth media. Phenotypically, growth was significantly slowed, virulence in Galleria mellonella was attenuated, and susceptibility to a variety of antibiotic classes was reduced when A. baumannii ATCC17978-VU was grown in minimal media versus rich media. Transcriptomic analysis showed differential regulation of >700 genes-including those associated with energy production and ribosomal function-when the two growth conditions were compared, with the majority of the upregulated genes seen in minimal media of unknown function. This study showed that culture media has a profound effect on the phenotype and cellular workings of a bacteria, highlighting the need for more studies of pathogens like A. baumannii ATCC17978-VU in minimal media.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-8"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial communities in the Hudson Strait amidst rapid environmental change. 快速环境变化中的哈德逊海峡微生物群落。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 DOI: 10.1139/cjm-2024-0154

Samantha J T Loutet, Alia Sanger, Kallie Strong, R Eric Collins, Nagissa Mahmoudi

引用次数: 0

Carvacrol, a monoterpenoid, binds quorum sensing proteins (LasI and LasR) and swarming motility protein BswR of Pseudomonas aeruginosa, resulting in loss of pathogenicity: an in silico approach. 单萜类香芹酚能与铜绿假单胞菌的法定量感应蛋白（LasI 和 LasR）和蜂群运动蛋白 BswR 结合，从而导致铜绿假单胞菌失去致病性：硅学方法

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-11-20 DOI: 10.1139/cjm-2024-0155

Susmita Datta, Vishal Singh, Soma Nag, Dijendra Nath Roy

{"title":"Carvacrol, a monoterpenoid, binds quorum sensing proteins (LasI and LasR) and swarming motility protein BswR of Pseudomonas aeruginosa, resulting in loss of pathogenicity: an in silico approach.","authors":"Susmita Datta, Vishal Singh, Soma Nag, Dijendra Nath Roy","doi":"10.1139/cjm-2024-0155","DOIUrl":"10.1139/cjm-2024-0155","url":null,"abstract":"The pathogenic Pseudomonas aeruginosa utilizes a quorum-sensing pathway for biofilm formation. The quorum-sensing proteins LasI and LasR of the Las system, alongside the swarming motility protein BswR, play a crucial role in the biofilm-mediated antibiotic resistance phenomenon. In this in silico study, LasI, LasR, and BswR were the prime targets for binding studies by promising drug candidates like linalool, ferutinin, citronellal, and carvacrol. These monoterpenoid compounds are carefully considered for this study due to their reported anti-microbial activity. Among all, carvacrol exhibited the highest binding energies with LasI (-5.932 kcal/mol), LasR (-7.469 kcal/mol), and BswR (-4.42 kcal/mol). Furthermore, the MMGBSA scores between carvacrol and LasI, LasR, and BswR individually are -33.14, -54.22, and -41.86 kcal/mol, which further corroborated the strong binding. During 100 ns of simulation, the ligand binds to the active sites of these proteins through the H-bonds at Ile107 of LasI, Tyr47 of LasR, and Leu57 of BswR. In addition, the root-mean-square deviation values of the ligand-protein complex are within the appropriate range of less than 5 Å. Absorption, Distribution, Metabolism, Excretion, and Toxicity analysis confirmed that carvacrol has the most negligible toxicity to mammalian cells. Hence, this finding is the first report to show that carvacrol can inhibit the Pseudomonas aeruginosa biofilms.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-15"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What in Earth? Analyses of Canadian soil populations of Aspergillus fumigatus. 地球上有什么？加拿大土壤曲霉菌群分析。

IF 1.8 4区生物学

Canadian journal of microbiology Pub Date : 2025-01-01 Epub Date: 2024-10-15 DOI: 10.1139/cjm-2024-0083

Greg Korfanty, Arshia Kazerouni, Mykaelah Dixon, Micheala Trajkovski, Paola Gomez, Jianping Xu

{"title":"What in Earth? Analyses of Canadian soil populations of Aspergillus fumigatus.","authors":"Greg Korfanty, Arshia Kazerouni, Mykaelah Dixon, Micheala Trajkovski, Paola Gomez, Jianping Xu","doi":"10.1139/cjm-2024-0083","DOIUrl":"10.1139/cjm-2024-0083","url":null,"abstract":"Aspergillus fumigatus is a globally distributed mold and a major cause of opportunistic infections in humans. Because most infections are from environmental exposure, it is critical to understand environmental populations of A. fumigatus. Soil is a major ecological niche for A. fumigatus. Here, we analyzed 748 soil isolates from 21 locations in six provinces and one territory in Canada. All isolates were genotyped using nine microsatellite markers. Due to small sample size and/or close proximities for some local samples, these isolates were grouped into 16 local geographic and ecological populations. Our results indicated high allelic and genotypic diversities within most local and provincial populations. Interestingly, low but statistically significant genetic differentiations were found among geographic populations within Canada, with relatively similar proportions of strains and genotypes belonging to two large genetic clusters. In Hamilton, Ontario, and Vancouver, BC, where two and three ecological populations were analyzed, respectively, we found limited genetic difference among them. Most local and provincial populations showed evidence of both clonality and recombination, with no population showing random recombination. Of the 748 soil isolates analyzed here, two were resistant to triazole antifungals. We discuss the implications of our results to the evolution and epidemiology of A. fumigatus.","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0