Bioresources and Bioprocessing最新文献

{"title":"Carob pod aqueous extract potentiates cisplatin efficacy and reduces toxicity in experimental hepatocellular carcinoma via mitochondrial and inflammatory pathway modulation.","authors":"Wael Sobhy Darwish, Abada El Sayed Khadr, Maher Abd El Naby Kamel, Tamer A Addissouky, Ahmed Zaki Ghareeb, Mabrouk A Abd Eldaim, Mohand K Razzaq, Ibrahim El Tantawy El Sayed, Hamed Mohamed Abdel-Bary, Doaa Ahmed Ghareeb","doi":"10.1186/s40643-026-01029-0","DOIUrl":"10.1186/s40643-026-01029-0","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is a global health challenge with limited therapeutic options. The effectiveness of conventional medication like cisplatin is often compromised by their severe toxicity. This study investigated carob pod aqueous extract (CPAE), a polyphenol-rich natural product, as a potential adjunct therapy to enhance efficacy and mitigate cisplatin toxicity in a preclinical HCC animal model.</p><p><strong>Methods: </strong>A rat model of HCC was established using diethylnitrosamine (DEN) and carbon tetrachloride (CCl₄). Forty-two Wistar rats were divided into seven groups, receiving various treatments: control, CPAE, vehicle, HCC only, HCC+CPAE, HCC+cisplatin, and HCC+CPAE+cisplatin. Liver and kidney function, metabolic profiles, oxidative stress/antioxidant parameters, gene and protein expression (AMPK, PGC-1α, TFAM, SIRT1, iNOS, NF-κB, IκK, p53, SREBP-2), histopathology, and statistical analyses were performed.</p><p><strong>Results: </strong>HCC induction caused significant liver dysfunction, metabolic disturbances, oxidative stress, alongside dysregulation of AMPK/PGC-1α/TFAM and NF-κB/iNOS pathways. CPAE, alone or with cisplatin, markedly ameliorated these changes, improving liver and kidney function, restoring antioxidant status, reducing the tumor marker AFP, suppressing pro-inflammatory and oncogenic signaling, and enhancing histological architecture. Furthermore, Combination therapy demonstrated synergistic benefits, with CPAE reducing cisplatin-induced nephrotoxicity and enhancing its antitumor efficacy, primarily via modulation of mitochondrial biogenesis, redox balance, and inflammatory signaling.</p><p><strong>Conclusions: </strong>CPAE exhibits potent hepatoprotective and anti-HCC activity, especially when combined with cisplatin. This combination modulates mitochondrial and inflammatory pathways while mitigating cisplatin-induced toxicity. These finding position CPAE as a promising natural adjuvant for integrative HCC management. Further translational studies are warranted to validate these findings and explore clinical applicability.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13287360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the therapeutic potential of gut microbiota metabolites for the treatment of renal fibrosis based on network pharmacology study.","authors":"Weiguo Yao, Jinlin Huo, Kun Liu, Pengyu Tao","doi":"10.1186/s40643-026-01083-8","DOIUrl":"10.1186/s40643-026-01083-8","url":null,"abstract":"<p><strong>Background: </strong>Renal fibrosis is a progressive injury contributing to renal function deterioration. Mounting evidence has underscored the profound impact of gut microbiota metabolites on host health and disease, yet their underlying mechanisms against renal fibrosis remain unclear. The aim of this study was to fully elucidate their therapeutic potential in the context of renal fibrosis.</p><p><strong>Methods: </strong>The targets of gut microbiota metabolites were identified in gutMGene. The diseases targets were obtained from the OMIM, GeneCards and DisGeNet databases. The STRING and DAVID platform were employed to identify the core targets and pathways. Gut Microbiota-Targets-Pathway-Metabolites (G-T-P-M) network was constructed to screen the core metabolites. Molecular docking was used to assess the interactions between the targets and metabolites.</p><p><strong>Results: </strong>A total of 47 overlapping targets related to gut microbiota metabolites and renal fibrosis were acquired. The bioinformatics analysis indicated that the targets were enriched in the regulation of TNF pathway and Toll-like receptor pathway. The PPI network (Protein-Protein Interaction) identified JUN, IL6, IL1B and AKT1 as the core targets. The G-T-P-M network revealed that Propionate, Butyrate and 3-Indolepropionic acid were identified as the core non-toxic and promising core metabolites. The core metabolites showed stable binding affinity with the core targets.</p><p><strong>Conclusion: </strong>The findings highlight that gut microbiota metabolites represent a promising therapeutic option for combating renal fibrosis by modulating multiple targets and pathways, providing a theoretical foundation for the future studies exploring gut microbiota as targeted strategies in the prevention and treatment of renal fibrosis.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13283118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the mechanism of Xinjiang Rosa laxa Retz.Fruit extract on IgA nephropathy by bioinformatics and machine learning methods.","authors":"Feng Qianqian, He Yuan, Guo Ying, Li Aoqing, Dilinur Kamili, Tian Li","doi":"10.1186/s40643-026-01090-9","DOIUrl":"10.1186/s40643-026-01090-9","url":null,"abstract":"<p><p>This study aimed to elucidate the core therapeutic targets and mechanisms of action of rosehip extract (FRLE) in IgA nephropathy (IgAN) by integrating bioinformatics, machine learning, and computational simulation techniques to simultaneously analyze the therapeutic effects of FRLE on IgAN. Through screening differentially expressed genes in IgAN patients and identifying potential target proteins of the core components of FRLE, 118 overlapping targets were ultimately determined as the gene set involved in the synergistic interaction between the two. Through triple machine learning validation, FGR and LDHB were identified as core target proteins, with Ellagic acid, Rutin, and Hyperoside as the core active components. In IgAN patients, LDHB was significantly downregulated, while FGR was significantly upregulated. Both demonstrated excellent diagnostic efficacy and were closely associated with the renal immune microenvironment. Molecular docking and molecular dynamics simulations confirmed that the core components could stably bind to FGR and LDHB. In summary, FRLE targets and modulates FGR and LDHB through its core components, intervening in inflammatory pathways and immune microenvironment imbalance, thereby providing a theoretical basis for subsequent experiments and clinical translation.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13276090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling anti-atherosclerotic targets of Perilla frutescens through a multi-scale computational framework integrating network pharmacology, single-cell analysis, machine learning, and molecular dynamics.","authors":"Chenchen Yang, Jianrong Xing, Mengzhu Wang, Wanyi Zhou, Ying Yang, Wenyang Tao","doi":"10.1186/s40643-026-01087-4","DOIUrl":"10.1186/s40643-026-01087-4","url":null,"abstract":"<p><p>Medicinal plants have long served as important sources of therapeutic agents owing to their diverse bioactive constituents and multi-target pharmacological properties. In particular, plant-derived compounds have attracted increasing attention for the management of chronic inflammatory and metabolic diseases, including atherosclerosis. However, the molecular mechanisms by which medicinal plants modulate the cellular heterogeneity and intercellular communication networks within atherosclerotic plaques remain insufficiently understood. Despite the widespread implementation of lipid-lowering therapy, the persistence of residual inflammatory risk, driven by immunometabolic network dysregulation, remains a cardinal therapeutic challenge in atherosclerosis (AS) management. While Perilla frutescens exhibits well-documented anti-inflammatory properties, the precise molecular targeting within the atherosclerotic plaque microenvironment and the regulatory mechanisms governing intercellular communication networks remain poorly elucidated. To address this gap, we established a multi-scale integrative computational framework synergizing network pharmacology, human atherosclerotic plaque single-cell transcriptomic (scRNA-seq) profiling, and ensemble machine learning algorithms (LASSO and random forest) for systematic identification of robust therapeutic targets. Subsequently, molecular docking coupled with 100-ns all-atom molecular dynamics (MD) simulations validated the binding affinity and thermodynamic stability of drug-target complexes. The study successfully analyzed the cellular heterogeneity lineage of plaques and identified a core feature set of 10 genes including HIF1A, PPARG and ITGB1, which specifically mapped the differentiation trajectory of macrophages to foam cells. External validation in an independent cohort demonstrated superior diagnostic performance of this signature (AUC = 0.996). Cellular communication network dissection revealed the foam cell-driven SPP1-ITGB1 signaling axis as a pivotal conduit orchestrating inflammatory crosstalk. Molecular docking demonstrated pronounced binding affinity between luteolin, the principal bioactive constituent of Perilla frutescens, and ITGB1 (binding energy: - 8.9 kcal/mol). MD simulations further corroborated the efficacy of luteolin in stabilizing ITGB1 conformation via a \"conformational-locking\" mechanism (RMSD equilibration within 0.10-0.20 nm), thereby abrogating pathological cell adhesion signaling transduction. Collectively, this study provides a high-resolution molecular atlas of Perilla frutescens-mediated AS intervention, systematically elucidating the mechanistic paradigm whereby luteolin attenuates vascular inflammation through targeted disruption of the SPP1-ITGB1 communication axis. These findings underscore the therapeutic targeting of cell adhesion receptors as a translationally promising strategy for mitigating residual inflammatory risk in AS.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable carotenoid production using amylaceous agro-industrial byproducts: process efficiency and environmental assessment.","authors":"Thércia Rocha Balbino, Salvador Sánchez-Muñoz, Stephanie Custódio Inácio, Gabriele Campelo Almeida, Ana Cláudia Dias, Júlio César Santos, Silvio Silvério da Silva, Jorge Fernando Brandão Pereira","doi":"10.1186/s40643-026-01086-5","DOIUrl":"10.1186/s40643-026-01086-5","url":null,"abstract":"<p><p>Agro‑industrial brans are abundant residues that can be valorized as low‑cost feedstocks for microbial bioprocesses within a circular‑economy framework. Here, we investigated the use of corn, soybean, rice and wheat bran hydrolysates as sole nutrient sources (no detoxification and no supplementation) for carotenoid production by the yeast Rhodotorula mucilaginosa. After standardizing total fermentable sugars to 10-12 g/L, the yeast successfully consumed mixed sugars and produced biomass and carotenoids in all hydrolysates. Rice bran hydrolysate led to the highest carotenoid titer (28.41 ± 0.23 mg/L) and the highest specific carotenoid content (1.11 ± 0.05 mg_carotenoids/g_{dry cells}), whereas soybean bran hydrolysate favored biomass formation (29.74 ± 0.18 g/L). UV-Vis/FTIR analyses of the pigment-rich extract showed spectral features consistent with carotenoids (absorption maxima at 480-490 nm and characteristic FTIR bands), supporting the qualitative identification of a carotenoid mixture. Finally, a cradle‑to‑gate life cycle assessment (LCA) showed that the RBH scenario presented the lowest impacts across the evaluated categories, with electricity demand during fermentation as the main hotspot. Overall, rice bran emerges as a promising residue for sustainable carotenoid bioproduction coupling process performance and environmental benefits. This study highlights the valorization of agro-industrial residues as an efficient strategy to reduce production costs and environmental impact, contributing to the development of more sustainable biotechnologies for carotenoid synthesis and beyond.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A strategy for high-yield lauric acid production in Hermetia illucens fed with pre-fermented sweet potato-based substrate.","authors":"Vivek Manyapu, Yo-Chia Chen","doi":"10.1186/s40643-026-01085-6","DOIUrl":"10.1186/s40643-026-01085-6","url":null,"abstract":"<p><p>The intensification of animal farming is associated with increased infectious and zoonotic disease risks. The black soldier fly larvae (BSFL) have antimicrobial activity that could potentially be used to suppress diseases and improve animal health. This study used carbohydrate-rich sweet potato and lauric acid-rich desiccated coconut to differentiate the primary source of lauric acid. The study also investigated the effect of pre-fermenting the substrates with Bacillus sp. and Klebsiella sp. on the BSFL growth performance and fatty acid composition, particularly on lauric acid. Correlation analyses were conducted between the larvae fed with fresh substrate and fermented substrate. Overall, the sweet potato enhanced the lauric acid biosynthesis, and the fermented sweet potato-based substrate resulted in a remarkable lauric acid production of 82%, i.e., 365 mg g^-1 in the BSFL biomass. Pre-fermentation aided production of precursors like oleic acid, linoleic acid, and glycolysis of readily available carbohydrates, which spiked the lauric acid biosynthesis.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13263363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in biomass deconstruction, microbial conversion, artificial intelligence, and carbon capture for sustainable bioenergy.","authors":"Vishwajit Kumar, Shikha Mishra, Pratham Joshi, Jyotsna Misra, Prakash Peralam Yegneswaran, Bhavanari Mallikarjun, Syed Shams Yazdani, Piyush Behari Lal","doi":"10.1186/s40643-026-01081-w","DOIUrl":"10.1186/s40643-026-01081-w","url":null,"abstract":"<p><p>The transition from fossil fuels to low-carbon bio-based energy systems is increasingly constrained by the efficiency, scalability, and integration of conversion technologies. Addressing this challenge, this review critically analyzes microbial biofuel production through a conversion-centric and systems-level framework, emphasizing how feedstock diversity, pretreatment chemistry, enzymatic deconstruction, and microbial metabolism collectively govern overall process performance. This review evaluates how pretreatment strategies modulate biomass recalcitrance, hydrolysate chemical ecologies, inhibitor profiles, and redox balance, thereby imposing fundamental constraints on enzymatic efficiency, microbial conversion yields, and emissions outcomes. Advances in enzymatic hydrolysis are assessed in terms of bond-specific catalysis, enzyme synergy, and persistent bottlenecks arising from substrate heterogeneity, lignin enzyme interactions, and non-productive binding, while microbial engineering strategies from robust monocultures to synthetic consortia and cell-free systems are examined through techno-economic and metabolic flux perspectives. It further highlights the emerging role of artificial intelligence and multi-omics integration in enabling predictive optimization of pretreatment severity, enzyme cocktails, and metabolic routing, moving beyond empirical process tuning. This article establishes a unified framework for integrated \"microbial lignocellulose-to-fuel\" pathways, demonstrating how coordinated advances in conversion technologies are essential for achieving scalable, economically viable, and environmentally sustainable bioenergy systems.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13260523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized extraction of Pinus taeda knotwood sawmill residues as source of antifungal compounds.","authors":"Rodrigo Coniglio, Juan Martín Rodao, Raquel Alonso, Facundo Fioritto, Karina Nicole Ruiz, Lucía Xavier, Jörn Appelt, María Noel Cabrera, Leonardo Clavijo","doi":"10.1186/s40643-026-01072-x","DOIUrl":"10.1186/s40643-026-01072-x","url":null,"abstract":"<p><p>In Uruguay, Pinus taeda is the dominant conifer in industrial plantations, generating large volumes of knot-containing offcuts during processing. These residues are highly enriched in extractives, offering opportunities for valorization within a circular bioeconomy. This study examined the recovery of bioactive compounds from P. taeda knotwood through ethanol extraction, process optimization, Gas Chromatography coupled with Mass Spectrometry and Flame Ionization Detection (GC-MS/FID) analysis, and antifungal evaluation. The effects of extraction temperature, ethanol concentration, and liquid-solid (L/S) ratio on extraction yield, total phenolic content, and FRAP antioxidant activity were assessed, and response models were developed to identify optimal conditions. Maximum extraction yield (20.8%) occurred at 47 °C, 100% ethanol concentration, and L/S of 8.3. The highest phenolic content (5.7 g gallic acid equivalents/100 g) was predicted at 61 °C, 100% ethanol, and L/S 15, while the greatest antioxidant capacity (15.8 mmol ascorbic acid equivalents/100 g) was achieved at 75 °C and L/S 15, independently of ethanol concentration. Two extraction conditions, representing the best compromise among yield, phenolics, and antioxidant performance, were selected for further characterization and antifungal tests. GC-MS/FID analysis showed that stilbenes and terpenoids dominated the extracts. Antifungal assays against Trametes versicolor and Gloeophyllum trabeum revealed strong inhibition, reaching up to 65% and 97% after seven days. Overall, the results demonstrate that P. taeda knotwood residues can be efficiently valorised via ethanolic extraction to obtain bioactive fractions with high antioxidant and antifungal activity, supporting sustainable and circular approaches for wood protection.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13246982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomass based carbon quantum dots in sensor applications: a review (2021-2025).","authors":"Elyor Berdimurodov, Khasan Berdimuradov, Ashish Kumar, Abhinay Thakur, Kamila Rashidova, Jasur Tursunqulov, Khudaybergan Polvonov, Alisher Ishankulov, Nafosat Oramova, Ahmad Hosseini-Bandegharaei, Bobirmirzo Khasanov, Rasulbek Eshmetov","doi":"10.1186/s40643-026-01084-7","DOIUrl":"10.1186/s40643-026-01084-7","url":null,"abstract":"<p><p>The past few years have witnessed an exponential rise in the development of carbon quantum dots (CQDs) derived from natural biomasses, rendering them a green and multifunctional platform for sensor applications. This comprehensive review provides a critical assessment of advancements between 2021 and 2025 in the syntheses, physicochemical characterizations, and sensing applications of biomass-derived CQDs. Different precursors-e.g., fruit peels, leaves, and agricultural waste-have been effectively transformed into highly fluorescent CQDs using hydrothermal, microwave-assisted, and pyrolytic routes, with synthesis times as short as 10 min for certain microwave procedures. The structural studies exhibit quasi-spherical morphologies (2-10 nm) and partial graphitization, and the optical studies confirm excitation-dependent fluorescence with quantum yields as high as 30%, particularly in nitrogen- and sulfur-doped systems. Biomass-derived CQDs have shown superb selectivity and sensitivity to a broad spectrum of analytes. For example, CQDs synthesized from Solanum nigrum leaves reported detection limits as low as 8 nM for Fe³⁺, while CQDs synthesized from pitaya peels enabled sensitive detection of antibiotics via aggregation-induced emission effects. Environmental targets such as glyphosate, NH₃, and CH₂O have also been detected at nanomolar levels, employing mechanisms like static/dynamic fluorescence quenching, electron transfer, and FRET. Despite these advances, there are still challenges in large-scale production, standardization of fabrication protocols, and integration of CQDs into real-time sensing platforms. In the future, the work is going to be further developed through the adoption of green synthesis approaches, regulatory standardizations, and integration of CQDs into wearable and portable diagnostic devices. Such developments point to the prospects of biomass-sourced CQDs as green, cost-effective, and ultrasensitive nanomaterials for next-generation chemical, biological, and environmental sensors.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13243163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-associated microbial succession and volatile flavor compound dynamics during cigar tobacco leaf fermentation.","authors":"Tianfei Zheng, Dongfeng Guo, Yaqi Shi, Jinlong Zhou, Cunyong Zhang, Kun Zong, Shaoxuan Ju, Xingjiang Li","doi":"10.1186/s40643-026-01060-1","DOIUrl":"10.1186/s40643-026-01060-1","url":null,"abstract":"<p><p>Temperature is a key factor driving microbial community succession and volatile flavor compound formation during the fermentation of cigar tobacco leaves (CTLs). This study systematically investigated microbial community dynamics, co-occurrence networks, and volatile flavor compound (VFCs) profiles of CTLs from Dominica and Yunnan under a 20-60 °C fermentation gradient. High-throughput sequencing identified Staphylococcus, Oceanobacillus, and thermophilic fungi as core microbes potentially associated with aroma formation. Dominica CTLs exhibiting higher microbial diversity than Yunnan CTLs. Dominica CTLs produced abundant esters, alcohols and ketones across different temperature stages, whereas Yunnan CTLs accumulated more pyrazines, indole and terpenoids at high temperatures (≥ 50 °C). Co-occurrence network analysis revealed temperature-driven shifts in microbial interactions: Dominica CTLs formed balanced networks with mixed positive/negative correlations at low temperatures, while Yunnan CTLs developed stable networks dominated by positive correlations at high temperatures. PERMANOVA indicated significant differences in microbial community structure among temperature gradients (R² = 0.78, p < 0.001). Spearman correlation analysis suggested that core microbes (e.g., Staphylococcus) were significantly correlated with the accumulation of key VFCs (e.g., esters, alcohols). These findings propose a conceptual temperature-microbe-VFC interaction framework, providing theoretical support for optimizing CTL fermentation processes.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148155370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}