Bioresources and Bioprocessing最新文献

{"title":"Scanning the active center of formolase to identify key residues for enhanced C1 to C3 bioconversion.","authors":"Guimin Cheng, Hongbing Sun, Qian Wang, Jinxing Yang, Jing Qiao, Cheng Zhong, Tao Cai, Yu Wang","doi":"10.1186/s40643-024-00767-3","DOIUrl":"10.1186/s40643-024-00767-3","url":null,"abstract":"<p><strong>Background: </strong>Formolase (FLS) is a computationally designed enzyme that catalyzes the carboligation of two or three C1 formaldehyde molecules into C2 glycolaldehyde or C3 dihydroxyacetone (DHA). FLS lays the foundation for several artificial carbon fixation and valorization pathways, such as the artificial starch anabolic pathway. However, the application of FLS is limited by its low catalytic activity and product promiscuity.</p><p><strong>Findings: </strong>FLS, designed and engineered based on benzoylformate decarboxylase from Pseudomonas putida, was selected as a candidate for modification. To evaluate its catalytic activity, 25 residues located within an 8 Å distance from the active center were screened using single-point saturation mutagenesis. A screening approach based on the color reaction of the DHA product was applied to identify the desired FLS variants. After screening approximately 5,000 variants (approximately 200 transformants per site), several amino acid sites that were not identified by directed evolution were found to improve DHA formation. The serine-to-phenylalanine substitution at position 236 improved the activity towards DHA formation by 7.6-fold. Molecular dynamics simulations suggested that the mutation increased local hydrophobicity at the active site, predisposing the cofactor-C2 intermediate to nucleophilic attack by the third formaldehyde molecule for subsequent DHA generation.</p><p><strong>Conclusions: </strong>This study provides improved FLS variants and valuable information into the influence of residues adjacent to the active center affecting catalytic efficiency, which can guide the rational engineering or directed evolution of FLS to optimize its performance in artificial carbon fixation and valorization.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"48"},"PeriodicalIF":4.6,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-production of 1,3-propanediol and phage phiKpS2 from the glycerol fermentation by Klebsiella pneumoniae.","authors":"Suyang Duan, Zhirong Zhang, Xiaoli Wang, Yaqin Sun, Yuesheng Dong, Lina Ren, Lili Geng, Zhilong Xiu","doi":"10.1186/s40643-024-00760-w","DOIUrl":"10.1186/s40643-024-00760-w","url":null,"abstract":"<p><p>As an alternative to antibiotics in response to antimicrobial-resistant infections, bacteriophages (phages) are garnering renewed interest in recent years. However, the massive preparation of phage is restricted using traditional pathogens as host cells, which incurs additional costs and contamination. In this study, an opportunistic pathogen, Klebsiella pneumoniae used to convert glycerol to 1,3-propanediol (1,3-PDO), was reused to prepare phage after fermentation. The phage infection showed that the fed-batch fermentation broth containing 71.6 g/L 1,3-PDO can be directly used for preparation of phage with a titer of 1 × 10⁸ pfu/mL. Then, the two-step salting-out extraction was adopted to remove most impurities, e.g. acetic acid (93.5%), ethanol (91.5%) and cells (99.4%) at the first step, and obtain 1,3-PDO (56.6%) in the top phase as well as phage (97.4%) in the middle phase at the second step. This integrated process provides a cheap and environment-friendly manner for coproduction of 1,3-PDO and phage.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"44"},"PeriodicalIF":4.6,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11082122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic digestion of process water from hydrothermal treatment processes: a review of inhibitors and detoxification approaches.","authors":"Mei Zhou, Kayode Taiwo, Han Wang, Jean-Nepomuscene Ntihuga, Largus T Angenent, Joseph G Usack","doi":"10.1186/s40643-024-00756-6","DOIUrl":"10.1186/s40643-024-00756-6","url":null,"abstract":"<p><p>Integrating hydrothermal treatment processes and anaerobic digestion (AD) is promising for maximizing resource recovery from biomass and organic waste. The process water generated during hydrothermal treatment contains high concentrations of organic matter, which can be converted into biogas using AD. However, process water also contains various compounds that inhibit the AD process. Fingerprinting these inhibitors and identifying suitable mitigation strategies and detoxification methods is necessary to optimize the integration of these two technologies. By examining the existing literature, we were able to: (1) compare the methane yields and organics removal efficiency during AD of various hydrothermal treatment process water; (2) catalog the main AD inhibitors found in hydrothermal treatment process water; (3) identify recalcitrant components limiting AD performance; and (4) evaluate approaches to detoxify specific inhibitors and degrade recalcitrant components. Common inhibitors in process water are organic acids (at high concentrations), total ammonia nitrogen (TAN), oxygenated organics, and N-heterocyclic compounds. Feedstock composition is the primary determinant of organic acid and TAN formation (carbohydrates-rich and protein-rich feedstocks, respectively). In contrast, processing conditions (e.g., temperature, pressure, reaction duration) influence the formation extent of oxygenated organics and N-heterocyclic compounds. Struvite precipitation and zeolite adsorption are the most widely used approaches to eliminate TAN inhibition. In contrast, powdered and granular activated carbon and ozonation are the preferred methods to remove toxic substances before AD treatment. Currently, ozonation is the most effective approach to reduce the toxicity and recalcitrance of N and O-heterocyclic compounds during AD. Microaeration methods, which disrupt the AD microbiome less than ozone, might be more practical for nitrifying TAN and degrading recalcitrant compounds, but further research in this area is necessary.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"47"},"PeriodicalIF":4.6,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11076452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-ultrasonic assisted extraction of lignin to synthesize new nano micellar organometallic surfactants for refining oily wastewater.","authors":"M H Alhalafi, S A Rizk, E S Al-Malki, A M Algohary","doi":"10.1186/s40643-024-00761-9","DOIUrl":"10.1186/s40643-024-00761-9","url":null,"abstract":"<p><p>In this work, a beneficial approach for efficient depolymerization of lignin and controllable product distribution is provided. Lignin, an abundant aromatic biopolymer, has the potential to produce various biofuels and chemical adsorption agents and is expected to benefit the future circular economy. Microwave-ultrasonic (MW/US) assisted efficient depolymerization of lignin affords some aromatic materials used in manufacturing the starting material to be investigated. Some nano organometallic surfactants (NOMS) based on Ni²⁺, Cu²⁺, Co²⁺, Fe³⁺, and Mn²⁺ besides 2-hydroxynaphth-sulphanilamide are synthesized to enhance oil recovery (EOR). In this work, the assessment of the NOMS's efficiency was improving the heavy oil recovery via the study of the dynamic interfacial tension (IFT), contact angle, and chemical flooding scenarios. The NOMS-Ni²⁺ exhibited the maximum reduction of viscosity and yield values. Dropping the viscosity to 819.9, 659.89, and 499.9 Pa s from blank crude oil viscosity of 9978.8, 8005.6, and 5008.6 Pa s respectively at temperatures of 40, 60, and 80 °C was investigated. The reduction of τ_B values was obtained also by OMS-Ni²⁺. The minimum IFT was recorded against the Ni²⁺ derivatives (0.1 × 10^-1 mN m^-1). The complete wettability alteration was achieved with the NOMS-Ni²⁺ surfactant (ɵ <math><mrow><mo>≅</mo> <mn>6.01</mn> <mo>)</mo> <mo>.</mo></mrow> </math> The flooding test has been steered in 3 sets using the sand-packed model as a porous media at surfactant concentrations (1, 1.5, 2 and 2.5%) at 50 °C and 499 psi as injection pressure. The best value (ORs) formed for NOMS-Ni²⁺ were 62, 81, 85.2, and 89% respectively as compared with other NOMS-M²⁺ at the same concentrations. The mechanism of alternating wettability was described in the text. The rheology of the used heavy crude oil was investigated under temperatures of 40, 60, and 80 °C.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"46"},"PeriodicalIF":4.3,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed analysis of agro-industrial byproducts/wastes to enable efficient sorting for various agro-industrial applications.","authors":"Govindegowda Priyanka, Jeevan R Singiri, Zachor Adler-Agmon, Sasank Sannidhi, Spurthi Daida, Nurit Novoplansky, Gideon Grafi","doi":"10.1186/s40643-024-00763-7","DOIUrl":"https://doi.org/10.1186/s40643-024-00763-7","url":null,"abstract":"<p><p>Agriculture-based industries generate huge amounts of byproducts/wastes every year, which are not exploited or disposed efficiently posing an environmental problem with implications to human and animal health. Finding strategies to increase the recycling of agro-industrial byproducts/wastes (AIBWs) is a primary objective of the current study. A thorough examination of AIBWs in conjunction with experimental research is proposed to facilitate sorting for various agro-industrial applications and consequently increasing byproduct/waste utilization. Accordingly, two sustainable, locally available sources of AIBWs, namely, wheat bran (WB) and garlic straw and peels (GSP) were studied in detail including content and composition of proteins, phytohormones and nutritional elements, as well as the effect of AIBW extracts on plant and microbial growth. Hundreds of proteins were recovered from AIBW mainly from WBs, including chaperons, metabolite and protein modifying enzymes, and antimicrobial proteins. In-gel assays showed that WB and GSP possess high protease and nuclease activities. Conspicuously, phytohormone analysis of AIBWs revealed the presence of high levels of strigolactones, stimulants of seed germination of root parasitic weeds, as well as indole acetic acid (IAA) and abscisic acid (ABA). Garlic straw extract strongly inhibited germination of the weed Amaranthus palmeri but not of Abutilon theophrasti and all examined AIBWs significantly affected post-germination growth. Bacterial growth was strongly inhibited by garlic straw, but enhanced by WBs, which can be used at least partly as a bacterial growth medium. Thus, an in-depth examination of AIBW characteristics will enable appropriate sorting for diverse agro-industrial applications, which will increase their utilization and consequently their economic value.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"45"},"PeriodicalIF":4.6,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11069496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water","authors":"Amer Jamal Hashmat, Muhammad Afzal, Samina Iqbal, Imran Amin, Carlos Alberto Arias, Hans Brix, Imran Zafar, Sania Riaz, Rizwan ur Rehman, Ahmad Mohammad Salamatullah, Gezahign Fentahun Wondmie, Mohammed Bourhia","doi":"10.1186/s40643-024-00757-5","DOIUrl":"https://doi.org/10.1186/s40643-024-00757-5","url":null,"abstract":"<p>The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) <i>alkB</i> gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of <i>Typha latifolia</i> and <i>Cyperus laevigatus</i> and inoculated with the bacterial consortium (<i>Pseudomonas putida</i> TYRI39, <i>Acinetobacter junii</i> TYRH47, <i>Acinetobacter</i> sp. CYRH17, <i>Pseudomonas</i> sp. CYSI27, and <i>Pseudomonas</i> sp. TYRH42). The mesocosms were provided with nutrients (20 mg l^− 1 N, 2.6 mg l^− 1 P, and 16.4 mg l^− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l^− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having <i>C. laevigatus</i>, bacteria, and NSA, and it is correlated with the copy numbers of <i>alkB</i> and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"9 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A straightforward chemobiocatalytic route for one-pot valorization of glucose into 2,5-bis(hydroxymethyl)furan","authors":"Xuan-Ping Liao, Qian Wu, Min-Hua Zong, Ning Li","doi":"10.1186/s40643-024-00758-4","DOIUrl":"https://doi.org/10.1186/s40643-024-00758-4","url":null,"abstract":"<p>Direct conversion of inexpensive biomass into value-added chemicals via furanic platform molecules is highly attractive. In this work, we present a straightforward chemobiocatalytic route for glucose valorization into 2,5-bis(hydroxymethyl)furan (BHMF) in one pot, with no purification of the intermediate 5-hydroxymethylfurfural (HMF). Six candidate alcohol dehydrogenase (ADH) genes were located from <i>Meyerozyma guilliermondii</i> SC1103, based on comparative transcriptome analysis and real-time quantitative polymerase chain reaction. An ADH (<i>Mg</i>ADH1) was identified upon evaluation of catalytic performances of recombinant <i>Saccharomyces cerevisiae</i> harboring candidate ADHs in HMF reduction. Soluble expression of the enzyme in <i>S. cerevisiae</i> was greatly enhanced by its codon optimization, leading to improved HMF tolerance (up to 400 mM). In a fed-batch process, the desired product of approximately 473 mM (60.5 g/L) was produced within 30 h by recombinant <i>S. cerevisiae</i>_<i>Mg</i>ADH1. A chemobiocatalytic route toward BHMF was constructed by merging CaCl₂-mediated isomerization and dehydration with biocatalytic reduction with an overall yield of approximately 42%, starting from glucose. This work may pave the way for green manufacture of valuable biobased chemicals.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"122 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the biosynthesis of polyunsaturated fatty acids by Rhodotorula mucilaginosa and Lodderomyces elongisporus","authors":"Amera A. Abaza, Yousseria M. Shetaia, Noha M. Sorour, Ashraf S. A. El-Sayed, Ashraf F. El-Baz","doi":"10.1186/s40643-024-00755-7","DOIUrl":"https://doi.org/10.1186/s40643-024-00755-7","url":null,"abstract":"<p>Single-cell oils (SCO) produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids. However, the accumulation of oils varies substantially between yeast spp. Consequently, identifying well-suited producers with a high innate capacity for lipids biosynthesis is paramount. Equally important is optimizing culturing and processing conditions to realize the total lipids production potential of selected strains. The marine <i>Rhodotorula mucilaginosa</i> and <i>Lodderomyces elongisporus</i> yeast were investigated to explore their potential for polyunsaturated fatty acids (PUFAs) production on high glucose media (HGM) using two-stage culture mode. Both strains accumulated &gt; 20% (w/w) of their dry cell weight as lipids when grown on HGM using a two-stage culture system. Both yeast isolates exhibited a maximal lipid/biomass coefficient (Y_L/X) of 0.58–0.66 mg/mg at 7 °C and 0.49–0.53 mg/mg at 26 °C when grown on 8% glucose and produced monounsaturated and PUFAs similar to that of Menhaden and Salmon marine oils. For the first time, significant amounts of Eicosapentaenoic acid (19%) and Eicosadienoic acid (19.6%) were produced by <i>L. elongisporus</i> and <i>R. mucilaginosa</i>, respectively. Thus, the SCO derived from these wild strains possesses significant potential as a substitute source for the industrial-scale production of long-chain PUFAs, making them a promising contender in the market.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"57 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris","authors":"Junyi Li, Manli Yang, Fengguang Zhao, Yaping Zhang, Shuangyan Han","doi":"10.1186/s40643-024-00752-w","DOIUrl":"https://doi.org/10.1186/s40643-024-00752-w","url":null,"abstract":"<p>Pectin lyase (PMGL) is an industrially important enzyme with widespread applications in the food, paper, and textile industries, owing to its capacity for direct degradation of highly esterified pectin. In this study, PMGL-Ba derived from <i>Bacillus licheniformis</i> underwent mining and heterologous expression in <i>P. pastoris.</i> Furthermore, diverse strategies, encompassing the optimization of expression cassette components, elevation of gene dosage, and co-expression of chaperone factors, were employed to augment PMGL-Ba production in <i>P. pastoris</i>. The signaling peptide OST1-pre-α-MF-pro and promoter AOX1 were finally selected as expression elements. By overexpressing the transcription factor Hac1p in conjunction with a two-copy PMGL-Ba setup, a strain yielding high PMGL-Ba production was achieved. In shake flask fermentation lasting 144 h, the total protein concentration reached 1.81 g/L, and the enzyme activity reached 1821.36 U/mL. For further scale up production, high-density fermentation transpired in a 5 L fermenter for 72 h. Remarkably, the total protein concentration increased to 12.49 g/L, and the enzyme activity reached an impressive 12668.12 U/mL. The successful heterologous and efficient expression of PMGL-Ba not only furnishes a valuable biological enzyme for industrial applications but also contributes to cost reduction in the utilization of biological enzymes in industrial applications.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"204 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant","authors":"Leticia F. Ngum, Y. Matsushita, Samir F. El-Mashtoly, Ahmed M. R. Fath El-Bab, Ahmed L Abdel-Mawgood","doi":"10.1186/s40643-024-00746-8","DOIUrl":"https://doi.org/10.1186/s40643-024-00746-8","url":null,"abstract":"<p>Cell separation using microfluidics has become an effective method to isolate biological contaminants from bodily fluids and cell cultures, such as isolating bacteria contaminants from microalgae cultures and isolating bacteria contaminants from white blood cells. In this study, bacterial cells were used as a model contaminant in microalgae culture in a passive microfluidics device, which relies on hydrodynamic forces to demonstrate the separation of microalgae from bacteria contaminants in U and W-shaped cross-section spiral microchannel fabricated by defocusing CO₂ laser ablation. At a flow rate of 0.7 ml/min in the presence of glycine as bacteria chemoattractant, the spiral microfluidics devices with U and W-shaped cross-sections were able to isolate microalgae (<i>Desmodesmus</i> sp.) from bacteria (<i>E. coli</i>) with a high separation efficiency of 92% and 96% respectively. At the same flow rate, in the absence of glycine, the separation efficiency of microalgae for U- and W-shaped cross-sections was 91% and 96%, respectively. It was found that the spiral microchannel device with a W-shaped cross-section with a barrier in the center of the channel showed significantly higher separation efficiency. Spiral microchannel chips with U- or W-shaped cross-sections were easy to fabricate and exhibited high throughput. With these advantages, these devices could be widely applicable to other cell separation applications, such as separating circulating tumor cells from blood.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"298 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}