Bioresources and Bioprocessing最新文献

{"title":"Blasting extrusion pretreatment of sweet sorghum bagasse for enhanced enzymatic saccharification and ethanol production using Pichia kudriavzevii ATCC 20,381.","authors":"Benjamín Vázquez-Rodríguez, Erick Heredia-Olea, Adriana Alamilla-Morales, Esther Pérez-Carrillo, David A Perez-Perez, Sergio O Serna-Saldívar","doi":"10.1186/s40643-025-00905-5","DOIUrl":"10.1186/s40643-025-00905-5","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"65"},"PeriodicalIF":4.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing.","authors":"Yu-Hsiu Li, Jiun-Jang Juo, I-Son Ng","doi":"10.1186/s40643-025-00907-3","DOIUrl":"10.1186/s40643-025-00907-3","url":null,"abstract":"<p><p>Atmospheric and Room Temperature Plasma (ARTP) mutagenesis has emerged as a novel and powerful physical mutation technology for microbial strain improvement recently. ARTP operates at atmospheric pressure and room temperature using a helium plasma jet, inducing widespread genomic mutations through reactive species and DNA damage. Compared to traditional mutagenesis methods, ARTP is safer, more efficient, and capable of producing high mutation rates without genetic modification, making it a valuable and sophisticated tool in biomanufacturing. This review outlines the principles and diverse applications of ARTP technology for enhancing enzyme activity, metabolite yields, and stress tolerance across various organisms. It also provides a comprehensive discussion of underlying biological mechanisms, workflow, optimization parameters, and potential cellular instability associated with ARTP-induced mutagenesis. Finally, current breakthroughs and future perspectives of ARTP mutagenesis are addressed, emphasizing its role in advancing next-generation microbial platforms for industrial biotechnology and environmental sustainability.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"63"},"PeriodicalIF":4.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of sugarcane bagasse: a comprehensive analysis for advanced energy conversion.","authors":"Nestor Proenza Pérez, Javier Alejandro Rodríguez Travieso, Elbis D Espaux Shelton, Daniel Travieso Pedroso, Einara Blanco Machin, Celso Eduardo Tuna, José Luz Silveira","doi":"10.1186/s40643-025-00878-5","DOIUrl":"10.1186/s40643-025-00878-5","url":null,"abstract":"<p><p>The sugarcane bagasse was analyzed for Particle Size Distribution (PSD) with a mean geometric diameter of 0.722 mm. Various standard techniques assessed its physical and chemical properties, including density measurements, higher heating value (HHV), thermogravimetric analysis (TGA/DTA), and compositional, proximate, ultimate, and CHNS/O analysis. The raw bagasse showed higher volatile matter, fixed carbon, ash content, and HHV of 16 MJ/kg, with lower moisture content (8.71%). Thermal analysis indicated a peak degradation temperature for organic matter at 310-330 °C, and bagasse exhibited a higher combustion index than fossil fuels and other biomasses. Logarithmic models were obtained to determine the real, particle, and apparent densities of bagasse with the mean particle size within the 0.075-9.5 mm range, showing adequate results for particles with a mean diameter greater than 0.15 mm. For smaller particles, the reported errors were 12.6%, 8.23%, and 28%, respectively. These findings highlight sugarcane bagasse's significant potential for thermochemical conversion systems and its importance in selecting and designing fluidized bed technologies like pneumatic conveying, drying, combustion, and gasification equipment.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"60"},"PeriodicalIF":4.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of pyrolysis conditions for Catha edulis waste-based biochar production using response surface methodology.","authors":"Abdi Birhanu, Abrha Mulu Hailu, Zemene Worku, Israel Tessema, Kenatu Angassa, Solomon Tibebu","doi":"10.1186/s40643-025-00866-9","DOIUrl":"10.1186/s40643-025-00866-9","url":null,"abstract":"<p><p>Catha edulis (Khat) waste (KW) is one of the challenging waste managements in Ethiopian urban areas. While biochar from other biomass sources has been studied, the effect of pyrolysis conditions on Catha edulis waste-based biochar yield and quality remains unexplored. Therefore, this study aims to optimize the biochar production process from Catha edulis waste for high yield and desirable characteristics. The KW and biochar were characterized using FTIR, BET, proximate analysis and other key parameters. The results indicated that KW possesses favorable properties for thermochemical conversion, with low ash content (4.35% wt. dry basis) and significant organic constituents (46.89% cellulose, 28.53% lignin, 19.62% hemicellulose, 4.96% extractives). The effect of pyrolysis process variables embracing reaction temperature, reaction time, and particle size on biochar yield and quality was optimized using response surface methodology (RSM) coupled with central composite design (CCD). The biochar was desirably characterized by a pH of 8.96, fixed carbon of 60.08%, ash content of 10.55%, and a yield of 45.12% at the optimum production processes of 390 °C, 44 min, and 0.7 mm particle size. Moreover, the study found that pyrolysis temperature was the most influential factor across all responses (yield and quality). Consequently, the biochar (yield and quality) was significantly (p < 0.05) influenced by pyrolysis temperature. In conclusion, the study inferred that KW holds substantial potential for biochar production with remarkable soil amendment characteristics.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"62"},"PeriodicalIF":4.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling optimization of cell growth cycle and key enzyme membrane localization for enhanced synthesis of high molecular weight heparosan by Corynebacterium glutamicum.","authors":"Jing Yu, Yang Zhang, He Zhang, Zemin Li, Zheng-Jun Li, Tianwei Tan","doi":"10.1186/s40643-025-00899-0","DOIUrl":"10.1186/s40643-025-00899-0","url":null,"abstract":"<p><p>High-molecular weight heparosan (HMW-heparosan) is a member of the glycosaminoglycan family. It possesses various chemical and physical properties suitable for a range of high-quality tissue engineering biomaterials, gels, scaffolds, and drug delivery systems. In this study, the HMW-heparosan biosynthesis pathway was engineered in Corynebacterium glutamicum through the introduction of heparosan synthase PmHS2 from Pasteurella multocida combined with overexpression of the key genes ugdA and galU, resulting in the generation of a stable HMW-heparosan-producing strain. Subsequently, to address metabolic flux competition, endogenous glycosyltransferases were systematically deleted to minimize UDP-glucose consumption, leading to a significant increase in HMW-heparosan accumulation. Additionally, cell growth was optimized by overexpressing transcriptional regulators whcD and PnkB, which was found to improve cell growth while creating an improved intracellular environment for biosynthesis. Notably, the critical enzyme heparosan synthase PmHS2 was relocated to the cell membrane by cell membrane display motifs porB, with its stability and catalytic efficiency being significantly enhanced so that the titer of HMW-heparosan reached 1.40 g/L in shake-flasks. Ultimately, the engineered strain was demonstrated to achieve HMW-heparosan production at 7.02 g/L with an average molecular weight (Mw) of 801 kDa in 5 L fed-batch bioreactor. These results demonstrate combinatorial optimization of cell factories, especially cell morphology and membrane localization of key enzymes, is efficacious and likely applicable for the production of other biopolymers.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"61"},"PeriodicalIF":4.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus subtilis spore surface display enhances manganese peroxidase stability and stress resistance.","authors":"Lu He, Mati Ullah, Muhammad Naeem, Zhong Ni, Yong Feng, Tawaf Ali Shah, Molalign Assefa, Khalid S Almaary, Huayou Chen","doi":"10.1186/s40643-025-00901-9","DOIUrl":"10.1186/s40643-025-00901-9","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"57"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Escherichia coli-driven whole-cell bioconversion for selective 5-Aminopentanol production as a novel bioplastic monomer.","authors":"Byung Wook Lee, Hee Taek Kim, Hyun Gi Koh, Kyungjae Yu, Gaeul Kim, Yoon Jung Jung, Haeng-Geun Cha, Yunhee Jeong, Yung-Hun Yang, See-Hyoung Park, Kyungmoon Park","doi":"10.1186/s40643-025-00904-6","DOIUrl":"10.1186/s40643-025-00904-6","url":null,"abstract":"<p><p>5-Aminopentanol (5-AP) is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics. Conventional production methods rely on petroleum-based feedstocks and metal catalysts, which raise environmental and sustainability concerns. In this study, a de novo biosynthetic pathway for 5-AP production from L-lysine was developed in Escherichia coli. The engineered pathway consisted of lysine decarboxylase 2 (LdcC), putrescine aminotransferase (PatA), and tested aldehyde reductase (YahK, YihU, YqhD). Among the tested reductases, aldehyde reductase exhibited the highest catalytic efficiency, producing 44.5 ± 2.6 mM of 5-AP (0.44 ± 0.03 mol_5 - AP/mol_l-lysine). The replacement of the expression system with a T7-based dual-plasmid platform, pET24ma::ldcC, and pCDFDuet-1::yqhD::patA co-transformed into E. coli, increased the production to 60.7 ± 5.8 mM, accompanied by reduced cadaverine accumulation. Further enhancement was achieved by increasing the gene dosage of PatA, leading to 68.5 ± 4.2 mM 5-AP and reduced by 40% in cadaverine levels. Cadaverine is a precursor in the production of 5-AP, and its accumulation is an important factor in the limitation of conversion to 5-AP. Intracellular cofactor regeneration is expected to cause an indirect supply of α-KG, a cofactor, to enhance conversion to 5-AP. To support intracellular cofactor regeneration, glucose supplementation and increased aeration were applied, resulting in a final titer of 78.5 ± 1.2 mM 5-AP and improved precursor utilization. This study is the first report of selective microbial 5-AP production and highlights the importance of PatA expression in pathway optimization. The newly established L-lysine (C6) valorization process which converts L-lysine to high-value materials such as 1,5-PDO, glutarate, and 5-AP offers a promising route for the sustainable biosynthesis of amino alcohols, laying the groundwork for future improvements through enzyme engineering and metabolic design.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"58"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting bacterial colonization and biofilm formation for enhanced biodegradation of low-density polyethylene microplastics.","authors":"Marwa Gamal Eldeen Afify, Ola M Gomaa, Hussein Abd El Kareem, Mohamed A Abou Zeid","doi":"10.1186/s40643-025-00902-8","DOIUrl":"10.1186/s40643-025-00902-8","url":null,"abstract":"<p><p>The accumulation of plastic waste presents a significant worldwide environmental challenge. This study aimed to isolate polyethylene-degrading bacteria from marine samples containing plastic waste. Four culturable bacterial isolates: Micrococcus luteus, Bacillus cereus, Enterococcus faecalis, and Actinomyces sp. were assessed for their biofilm formation, biosurfactant, and protease production. Gamma irradiation was used to induce structural changes and promote bacterial colonization and biofilm formation on low-density polyethylene microplastics (LDPE MPs). Optimal biofilm formation was achieved in minimal media supplemented with 30% tryptic soy broth, 10% biosurfactant, and 300 µM calcium chloride. The factorial design experiment demonstrated that adding media supplementation significantly improved bacterial colonization and biofilm formation when compared to gamma irradiation. This was supported with Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) mapping, and Fourier Transform Infrared Spectroscopy (FTIR). The optimized LDPE MP degradation was achieved through a multi-step protocol: (1) samples are pre-treated to 40 kGy gamma irradiation, which resulted in 5.7% Gravimetric weight loss and structural and morphological changes, (2) incubation in biofilm inducing media overnight, and (3) further incubation in minimal media for 30 days. This approach resulted in a total weight loss of 22.5%. In conclusion, synergistic pre-treatment is recommended to promote biofilm and improve biodegradation of LDPE MPs by marine bacteria.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"59"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive preservative nano-packaging films based on food wastes of orange peels and Shrimp for apple (Malus domestica var. Anna) fruit quality and storage.","authors":"Mohamed S Hasanin, Mahmoud Emam, M A Ahmed, F M Rohim, M A A Mohamed, Housni El Saied, Hamdy A Z Hussein, A Abdelkhalek","doi":"10.1186/s40643-025-00890-9","DOIUrl":"10.1186/s40643-025-00890-9","url":null,"abstract":"<p><p>Storage of economic fruits is a fatal economic and nutritional factor for most countries. Edible coating played a restricted role in this manner, forcing good usability with many limitations. In this work, Apple (Malus domestica var. Anna) was coated using bioactive nanopackaging films formulated to overcome the economic limitations and drawbacks of conventional coating. The formulated bioactive nanopackaging was based on nanochitosan prepared from shrimp shells and orange peel waste, which is used to produce nanocellulose (white part) and extract active ingredients (orange part). The formulated bioactive nanopackaging based on nanochitosan and nanocellulose (2:1) and orange peel waste extract with ratios of 1, 3, and 5% (w/w) based on nanochitosan dry weight and called T3, T4, and T5, respectively. Characteristics of bioactive nanopackaging films and their precursor materials were characterized physicochemically and topographically as well. The waste orange peel waste extract was characterized phytochemically. According to the orange peel extract, the formulated bioactive nanopackaging films observed antioxidant and antimicrobial activity. The results revealed that all treatments outperformed the control, especially treatments T4 (1% Nano Chitosan + 1% Nano Cellulose + 3% Orange Peel Waste extract) and T5 (1% Nano Chitosan + 1% Nano Cellulose + 5% Orange Peel Waste extract), in terms of fruit decay percentage (11.72 ± 11.4 C and 12.33 ± 10.83 C, respectively), weight loss (3.81 ± 2.29B and 3.77 ± 2.22B, respectively), TSS/acidity (17.07 ± 1.14 A and 16.77 ± 1.18 A, respectively), fruit firmness (12.83 ± 1.19B and 13.48 ± 0.91 A, respectively), total sugars (7.98 ± 0.21 A and 8.21 ± 0.29 A, respectively) and total anthocyanin (0.21 ± 0.03B and 0.25 ± 0.05 A, respectively).</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"54"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling anti-atherosclerosis mechanism of anthocyanins from Xinjiang wild cherry plum (Prunus divaricata Ledeb) via network pharmacology and molecular docking.","authors":"Siyu Li, Juan He, Huiyi Hu, Guang Wang, Juan Tang, Jun Yao, Jing Shen, Xing Li","doi":"10.1186/s40643-025-00900-w","DOIUrl":"10.1186/s40643-025-00900-w","url":null,"abstract":"<p><p>Atherosclerosis is a chronic vascular disease characterized by failure to resolve inflammation and forming plaque within the arterial wall. Atherosclerosis and its related cardiovascular diseases are the major causes of death worldwide. Our previous preliminary study showed that anthocyanin-rich extract (ACNE) from Xinjiang wild cherry plum (Prunus divaricata Ledeb) fruit peels exhibited anti-atherosclerotic effect. However, the potential mechanism of this health-beneficial effect remains unclear. Here, network pharmacology combined with molecular docking was used to tentatively address this issue. The ACNE mainly contains cyanidin, cyanidin 3-glucoside (Cy3Glu), Cyanidin 3-(6''-acetylglucoside) (Cy3AcGlu), cyanidin 3-galactoside (Cy3Gal), cyanidin 3-xyloside (Cy3Xyl), and cyanidin 3-rutinoside (Cy3Rut). Seven key targets, EGFR, VEGFA, HSP90AA1, SRC, HIF1A, CXCR4 and IGF1R were identified from core protein-protein interaction (PPI) network. Anthocyanins interacting on key targets were initially demonstrated by molecular docking, particularly Cy3Rut and Cy3Xyl having highest affinity with most key targets. Biological function analysis suggested that key targets were involved in several biological processes, including positive regulation of cell migration, positive regulation of phosphorylation, inflammatory response, response to hypoxia, etc. The significantly enriched pathways, such as HIF-1 signaling pathway, calcium signaling pathway, macrophage stimulating protein MSP signaling network map, were closely related to atherosclerosis. Altogether, based on the comprehensive analysis and discussion, we revealed that TLR4/EGFR and IGF1R-CXCL12/CXCR4 pathways were at least partially implicated in the anti-atherosclerotic effects of anthocyanins through affecting inflammation, endothelial homeostasis, and foam cell formation. This study served as a theoretical basis for further validating the underlying anti-atherosclerotic mechanism of anthocyanins via in vitro and in vivo experiments.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"53"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}