Bioresources and Bioprocessing最新文献

Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst.

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-31 DOI: 10.1186/s40643-025-00840-5

Joana T Cunha, Aloia Romaní, Lucília Domingues

{"title":"Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst.","authors":"Joana T Cunha, Aloia Romaní, Lucília Domingues","doi":"10.1186/s40643-025-00840-5","DOIUrl":"10.1186/s40643-025-00840-5","url":null,"abstract":"Background: There is an urgent need to develop bioprocesses independent of fossil resources to address resource depletion and mitigate environmental harm. Transitioning to a bio-based economy requires prioritizing chemical production processes that utilize renewable resources, ensuring sustainability and environmental responsibility. 5-Hydroxymethylfurfural (HMF) and its derivatives are promising building blocks, ranked among the top 12 bio-based molecules derived from biomass. This study investigates the potential of wine residues as substrates for HMF production and explores the yeast Saccharomyces cerevisiae, a robust industrial microbial cell factory, as a whole-cell biocatalyst for converting HMF into high-value compounds, offering an alternative to chemical synthesis.Findings: Several S. cerevisiae strains were compared for their ability to convert HMF, demonstrating varying capacities for oxidation or reduction. For the first time, HMF derivatives with potential industrial applications were produced using an HMF-rich hydrolysate obtained from sustainable processing of wine-growing waste, such as grape pomace and must surplus. The selected yeast strain was engineered to express the oxidoreductase enzyme of HMF/Furfural from Cupriavidua basilensis strain HMF14, resulting in a 15-fold increase in the accumulation of oxidized derivatives such as 2,5-furandicarboxylic acid (FDCA).Conclusions: These findings highlight the potential of leveraging wine residues and engineered S. cerevisiae strains to develop sustainable bioprocesses for producing valuable HMF derivatives, thereby contributing to the advancement of bio-based chemical production.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"8"},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mining versatile feruloyl esterases: phylogenetic classification, structural features, and deep learning model.

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-29 DOI: 10.1186/s40643-024-00835-8

Liang Guo, Yuxin Dong, Deyong Zhang, Xinrong Pan, Xinjie Jin, Xinyu Yan, Yin Lu

{"title":"Mining versatile feruloyl esterases: phylogenetic classification, structural features, and deep learning model.","authors":"Liang Guo, Yuxin Dong, Deyong Zhang, Xinrong Pan, Xinjie Jin, Xinyu Yan, Yin Lu","doi":"10.1186/s40643-024-00835-8","DOIUrl":"10.1186/s40643-024-00835-8","url":null,"abstract":"Feruloyl esterases (FEs, EC 3.1.1.73) play a crucial role in biological synthesis and metabolism. However, the identification of versatile FEs, capable of catalyzing a wide range of substrates, remains a challenge. In this study, we obtained 2085 FE sequences from the BRENDA database and initiated with an enzyme similarity network analysis, revealing three main clusters (1-3). Notably, both cluster 1 and cluster 3 included the characterized FEs, which exhibited significant differences in sequence length. Subsequent phylogenetic analysis of these clusters unveiled a correlation between phylogenetic classification and substrate promiscuity, and enzymes with broad substrate scope tended to locate within specific branches of the phylogenetic tree. Further, molecular dynamics simulations and dynamical cross-correlation matrix analysis were employed to explore structural dynamics differences between promiscuous and substrate-specific FEs. Finally, to expand the repertoire of versatile FEs, we employed deep learning models to predict potentially promiscuous enzymes and identified 38 and 75 potential versatile FEs from cluster 1 and cluster 3 with a probability score exceeding 90%. Our findings underscore the utility of integrating phylogenetic and structural features with deep learning approaches for mining versatile FEs, shedding light on unexplored enzymatic diversity and expanding the repertoire of biocatalysts for synthetic applications.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"7"},"PeriodicalIF":4.3,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Up-flow anaerobic sludge blanket bioreactor for the production of carboxylates: effect of inocula on process performance and microbial communities.

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-24 DOI: 10.1186/s40643-025-00839-y

Adrián Lago, Silvia Greses, Inés Moreno, Cristina González-Fernández

{"title":"Up-flow anaerobic sludge blanket bioreactor for the production of carboxylates: effect of inocula on process performance and microbial communities.","authors":"Adrián Lago, Silvia Greses, Inés Moreno, Cristina González-Fernández","doi":"10.1186/s40643-025-00839-y","DOIUrl":"10.1186/s40643-025-00839-y","url":null,"abstract":"This research investigated the acidogenic fermentation (AF) of sugar cane molasses in an up-flow anaerobic sludge blanket (UASB) reactor for the production of carboxylates. The first step was to assess the optimum process temperature (25, 35 or 55 ºC) using two different granular inocula, one from a brewery company (BGS) and other from a paper plant company (PGS). These experiments determined that the most suitable temperature for carboxylates production was 25 ºC, obtaining higher bioconversions (27.3 ± 0.3% using PGS and 39.2 ± 0.2% using BGS), despite the low pH value recorded (4.0-4.2). Then, both inocula were tested in UASB reactors. As a consequence of the operational conditions (25 ºC, pH = 5.5-6, organic loading rate (OLR) = 3 gCOD·L-1·d-1 and hydraulic retention time (HRT) = 10 d), the microbial communities changed from those typical for biogas production to those specialised in the production of volatile fatty acids (VFAs). Indeed, the highest bioconversion efficiency (70.1%) was obtained with BGS, where uncultured Eubacteriaceae family microorganisms (56.0%) prevailed, enhancing the production of butyric acid (59.5 ± 2.4%w/w). Consequently, this inoculum was used to further identify the OLR threshold that should not be exceeded to attain optimal carboxylates production. OLR of 6 gCOD·L-1·d-1 resulted in a decrease in bioconversion efficiency (59.5%). The VFAs pool was dominated by butyric acid (63.0 ± 1.4%w/w at an OLR of 4.5 gCOD·L-1·d-1 and 52.8 ± 2.2%w/w at 6 gCOD·L-1·d-1). The microbial community became even more specialised, increasing the presence of Firmicutes and Actinobacteriota phyla, proving that the imposed conditions favoured the production of VFAs when operating semicontinuously fed UASB reactors.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"6"},"PeriodicalIF":4.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield. 利用系统代谢工程技术在脂耶氏菌中重新合成槲皮素以提高产量。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-22 DOI: 10.1186/s40643-024-00825-w

Yuxing Dong, Wenping Wei, Mengfan Li, Tao Qian, Jiayun Xu, Xiaohe Chu, Bang-Ce Ye

{"title":"De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield.","authors":"Yuxing Dong, Wenping Wei, Mengfan Li, Tao Qian, Jiayun Xu, Xiaohe Chu, Bang-Ce Ye","doi":"10.1186/s40643-024-00825-w","DOIUrl":"10.1186/s40643-024-00825-w","url":null,"abstract":"Kaempferol and quercetin possess various biological activities, making them valuable in food and medicine. However, their production via traditional methods is often inefficient. This study aims to address this gap by engineering the yeast Yarrowia lipolytica to achieve high yields of these flavonoids. We designed a kaempferol biosynthetic pathway by integrating multiple-copy fusion enzyme expression modules, F3H-(GGGGS)2-FLS, into the genome with an optimized linker (GGGGS)2 to enhance kaempferol production from naringenin. To synthesize quercetin de novo, we introduced the FMOCPR gene into the kaempferol-synthesizing strain using the optimized pFBAin promoter. Notably, increasing glucose concentration effectively boosted the production of both flavonoids. Our results demonstrated kaempferol and quercetin titers reaching 194.30 ± 7.69 and 278.92 ± 11.58 mg/L, respectively, in shake-flask cultures. These findings suggest that Y. lipolytica is a promising platform for the efficient production of flavonoid-derived products.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"5"},"PeriodicalIF":4.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening. 基于转录调控因子SerR定向进化的l -苏氨酸和l -脯氨酸生物传感器工程及其在高通量筛选中的应用

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-19 DOI: 10.1186/s40643-024-00837-6

Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun

{"title":"Engineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening.","authors":"Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun","doi":"10.1186/s40643-024-00837-6","DOIUrl":"10.1186/s40643-024-00837-6","url":null,"abstract":"Amino acids are important bio-based products with a multi-billion-dollar market. The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of high-performance amino acid producers. However, there remains a pressing need for biosensors that specifically target certain critical amino acids, such as L-threonine and L-proline. In this study, a novel transcriptional regulator-based biosensor for L-threonine and L-proline was successfully developed, inspired by our new finding that SerE can export L-proline in addition to the previously known L-threonine and L-serine. Through directed evolution of SerR (the corresponding transcriptional regulator of SerE), the mutant SerRF104I which can recognize both L-threonine and L-proline as effectors and effectively distinguish strains with varying production levels was identified. Subsequently, the SerRF104I-based biosensor was employed for high-throughput screening of the superior enzyme mutants of L-homoserine dehydrogenase and γ-glutamyl kinase, which are critical enzymes in the biosynthesis of L-threonine and L-proline, respectively. A total of 25 and 13 novel mutants that increased the titers of L-threonine and L-proline by over 10% were successfully identified. Notably, six of the newly identified mutants exhibited similarities to the most effective mutants reported to date, indicating the promising application potential of the SerRF104I-based biosensor. This study illustrates an effective strategy for the development of transcriptional regulator-based biosensors for amino acids and other chemical compounds.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"4"},"PeriodicalIF":4.3,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on mushroom mycelium-based products and their production process: from upstream to downstream. 蘑菇菌丝体产品及其生产工艺综述：从上游到下游。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-10 DOI: 10.1186/s40643-024-00836-7

Hyun-Jae Shin, Hyeon-Su Ro, Moriyuki Kawauchi, Yoichi Honda

{"title":"Review on mushroom mycelium-based products and their production process: from upstream to downstream.","authors":"Hyun-Jae Shin, Hyeon-Su Ro, Moriyuki Kawauchi, Yoichi Honda","doi":"10.1186/s40643-024-00836-7","DOIUrl":"10.1186/s40643-024-00836-7","url":null,"abstract":"The global trend toward carbon neutrality and sustainability calls for collaborative efforts in both the basic and applied research sectors to utilize mushroom mycelia as environmentally friendly and sustainable materials. Fungi, along with animals and plants, are one of the major eukaryotic life forms. They have long been utilized in traditional biotechnology sectors, such as food fermentation, antibiotic production, and industrial enzyme production. Some fungi have also been consumed as major food crops, such as the fruiting bodies of various mushrooms. Recently, new trends have emerged, shifting from traditional applications towards the innovative use of mushroom mycelium as eco-friendly bioresources. This approach has gained attention in the development of alternative meats, mycofabrication of biocomposites, and production of mycelial leather and fabrics. These applications aim to replace animal husbandry and recycle agricultural waste for use in construction and electrical materials. This paper reviews current research trends on industrial applications of mushroom mycelia, covering strain improvements and molecular breeding as well as mycelial products and the production processes. Key findings, practical considerations, and valorization are also discussed.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"3"},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11723872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategy of employing plug-and-play vectors and LC-MS screening to facilitate the discovery of natural products using Aspergillus oryzae. 利用即插即用载体和LC-MS筛选促进米曲霉天然产物发现的策略。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-08 DOI: 10.1186/s40643-024-00833-w

Hanliang Shi, Beibei Lin, Mengmeng Zheng, Fengyu Gan, Zhi Lin, Xiujuan Xin, Jian Zhao, Xudong Qu, Faliang An

引用次数: 0

Effect of catalase on CPC production during fermentation of Acremonium chrysogenum. 过氧化氢酶对发酵酶产CPC的影响。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-01-04 DOI: 10.1186/s40643-024-00831-y

Ling Liu, Zhen Chen, Xiwei Tian, Ju Chu

{"title":"Effect of catalase on CPC production during fermentation of Acremonium chrysogenum.","authors":"Ling Liu, Zhen Chen, Xiwei Tian, Ju Chu","doi":"10.1186/s40643-024-00831-y","DOIUrl":"10.1186/s40643-024-00831-y","url":null,"abstract":"Cephalosporin C (CPC) is a critical raw material for cephalosporin antibiotics produced by Acremonium chrysogenum. During fermentation, the oxygen supply is a crucial factor limiting the efficient biosynthesis of CPC. This study demonstrated that the addition of exogenous surfactants significantly increased the dissolved oxygen (DO) level, extracellular catalase content, and final CPC titer. Consequently, we hypothesized and examined a correlation between catalase and CPC biosynthesis in A. chrysogenum through both the exogenous addition of hydrogen peroxide (H₂O₂) and the endogenous modulation of the catA expression level. The results indicated that both the addition of H₂O₂ and the ∆catA mutation exhibited similar fermentation trends, leading to decreased extracellular catalase activity and increased intracellular reactive oxygen species (ROS) content, which resulted in reduced CPC production. Conversely, strains that overexpress varying levels of the catA accelerated hyphal differentiation under DO-limiting conditions, reducing intracellular ROS accumulation and decreasing cellular apoptosis, which stabilized CPC yield during the later stages of fermentation. This study provides a critical foundation for further investigations into the regulatory mechanisms governing CPC biosynthesis in A. chrysogenum.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"1"},"PeriodicalIF":4.3,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fates of nutrient elements and heavy metals during thermal conversion of cattle slurry-derived anaerobic digestates. 牛浆液厌氧消化液热转化过程中营养元素和重金属的命运。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-12-30 DOI: 10.1186/s40643-024-00828-7

Daniel J Lane, Olli Sippula, Jorma Jokiniemi, Mikko Heimonen, Niko M Kinnunen, Perttu Virkajärvi, Narasinha Shurpali

{"title":"Fates of nutrient elements and heavy metals during thermal conversion of cattle slurry-derived anaerobic digestates.","authors":"Daniel J Lane, Olli Sippula, Jorma Jokiniemi, Mikko Heimonen, Niko M Kinnunen, Perttu Virkajärvi, Narasinha Shurpali","doi":"10.1186/s40643-024-00828-7","DOIUrl":"10.1186/s40643-024-00828-7","url":null,"abstract":"Thermal processes are emerging as promising solutions to recovering phosphorus and other nutrient elements from anaerobic digestates. The feasibility of nutrient element recovery depends largely on the fates of nutrient elements and heavy metals during thermal processing. This study assesses the partitioning of macronutrients (N, P, K, Na, Ca and Mg) and heavy metals (Zn, Cu, and Mn) between condensed and gaseous phases during thermal conversion of cattle slurry digestates in gas atmospheres of pyrolysis, combustion, and gasification processes. This study also assesses the chemical forms of macronutrients retained in combustion ashes. The partitioning of elements between condensed and gaseous phases was quantified by mass balances based on elemental analyses of char and ash residues. The char and ash residues were prepared in a fixed-bed, batch reactor at temperatures within the range 800-1000 °C. Powder X-ray diffraction was used to identify the chemical forms of macronutrient elements in combustion ashes. Volatilisation of P was low (< 20%) when the digestates were heated in inert and oxidising atmospheres, whereas a reducing atmosphere volatilized P to a major extent (~ 60% at 1000 °C). Oxidising atmospheres increased volatilisation of N but suppressed volatilisation of K, Na, and Zn. Volatilisation of the following elements was low (< 30%) in all investigated operating conditions: Ca, Mg, Mn, and Cu. The combustion ashes contained both high concentrations of P (around 7 w/w%) and acceptable concentrations of regulated heavy metals (Cu, and Zn) for application on agricultural and forest soils in Finland. Phosphorous was retained in the combustion ashes in the form of whitlockite. This form of P is expected to be available to plants when the ashes are added to soil.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"115"},"PeriodicalIF":4.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrolysis of palm kernel meal fibre using a newly isolated Bacillus subtilis F6 with high mannanase activity. 利用新分离的具有高甘露聚糖酶活性的枯草芽孢杆菌F6水解棕榈仁粕纤维。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-12-25 DOI: 10.1186/s40643-024-00826-9

Wei Li Ong, Kam Lock Chan, Antonius Suwanto, Zhi Li, Kian-Hong Ng, Kang Zhou

{"title":"Hydrolysis of palm kernel meal fibre using a newly isolated Bacillus subtilis F6 with high mannanase activity.","authors":"Wei Li Ong, Kam Lock Chan, Antonius Suwanto, Zhi Li, Kian-Hong Ng, Kang Zhou","doi":"10.1186/s40643-024-00826-9","DOIUrl":"10.1186/s40643-024-00826-9","url":null,"abstract":"Palm kernel meal (PKM) presents a challenge for non-ruminant livestock feeding due to its high fibre content predominantly in the form of mannan. Microbial fermentation offers a sustainable solution for fibre hydrolysis in lignocellulosic biomass. In this study, a Bacillus subtilis strain (F6), with high mannanase secretion capability, was isolated from the environment. Fermentation of PKM with B. subtilis F6 resulted in at least a 10% reduction in neutral detergent fibre, decreasing from 78.4 to 60.9% within 24 h. Notably, B. subtilis F6 rapidly responded to PKM, producing significant mannanase activity within 6 h, facilitating quick fibre degradation. Transcriptome analysis identified key enzymes involved in this process, with β-mannanase GmuG showing the highest increase in expression (45.2-fold) after fermentation. Purified recombinant GmuG exhibited strong PKM hydrolysis activity, primarily releasing mannobiose and mannotriose. Characterization of GmuG using locust bean gum as a substrate revealed an optimum temperature of 50-55 °C and pH optima at 5.0 and 9.0. This study highlights the potential of B. subtilis F6 and its mannanase GmuG for efficient PKM fibre hydrolysis, and provides insights into their application in the valorization of mannan-rich bioresources.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"113"},"PeriodicalIF":4.3,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0