Bioresources and Bioprocessing最新文献

Green synthesis of calcium oxide nanocatalyst and application in transesterification of waste cooking oil. 氧化钙纳米催化剂的绿色合成及其在废食用油酯交换中的应用。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-06-02 DOI: 10.1186/s40643-025-00879-4

Rajni Garg, Rishav Garg, Nnabuk Okon Eddy, Mukhtar Iderawumi Abdulraheem, Oluwadamilola Oluwatoyin Hazzan, Gholaremza Abdi

{"title":"Green synthesis of calcium oxide nanocatalyst and application in transesterification of waste cooking oil.","authors":"Rajni Garg, Rishav Garg, Nnabuk Okon Eddy, Mukhtar Iderawumi Abdulraheem, Oluwadamilola Oluwatoyin Hazzan, Gholaremza Abdi","doi":"10.1186/s40643-025-00879-4","DOIUrl":"https://doi.org/10.1186/s40643-025-00879-4","url":null,"abstract":"This study aims to synthesize calcium oxide nanoparticles by employing green synthetic methods and explore their potential as nano-catalyst based upon the utilization of waste into a value-based product. Waste orange peel extract has been utilized as a reducing medium. The reaction was optimized by varying the reactants' molar ratio to obtain calcium carbonate microparticles that were calcined to obtain calcium oxide nanoparticles with a particle size ranging from 70 to 100 nm. Various spectrochemical techniques analyzed the composition and morphology of the nano-catalyst. The nano-catalyst was further exploited in the one-pot transesterification of waste cooking oil. The biodiesel was analyzed for the presence of methyl ester groups by FTIR and GCMS analysis. The impact of varying reaction constraints, including temperature, contact time, nano-catalyst concentration, and methanol-oil molar ratio, were critically analyzed to optimize biodiesel yield. The study provided an economical and environmentally benign technique to successfully synthesize calcium oxide nano-catalyst to obtain biodiesel with 93.4% yield and effective waste minimization.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"52"},"PeriodicalIF":4.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on techno-economic assessment of Spirulina for sustainable nutraceutical, medicinal, environmental, and bioenergy applications. 螺旋藻在营养、医药、环境和生物能源领域的可持续应用技术经济评价综述。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-06-02 DOI: 10.1186/s40643-025-00888-3

Musa Nasiru Musa, Ghazali Musa Jirgi, Zakariyya Uba Zango, Marnawi Nasiru Isah, Muhammad Abdurrazak, Adamu Ahmad Adamu, Ismael A Wadi, Adekunle Akanni Adeleke, Zaharaddeen N Garba, Usman Bello, Haruna Adamu, Ahmad Hosseini-Bandegharaei, Dmitry Olegovich Bokov

{"title":"A review on techno-economic assessment of Spirulina for sustainable nutraceutical, medicinal, environmental, and bioenergy applications.","authors":"Musa Nasiru Musa, Ghazali Musa Jirgi, Zakariyya Uba Zango, Marnawi Nasiru Isah, Muhammad Abdurrazak, Adamu Ahmad Adamu, Ismael A Wadi, Adekunle Akanni Adeleke, Zaharaddeen N Garba, Usman Bello, Haruna Adamu, Ahmad Hosseini-Bandegharaei, Dmitry Olegovich Bokov","doi":"10.1186/s40643-025-00888-3","DOIUrl":"https://doi.org/10.1186/s40643-025-00888-3","url":null,"abstract":"Global population growth underlies the need to explore alternative materials to address pressing challenges in food security, medicine, energy, and environmental pollution. Spirulina is a nutrient dense cyanobacteria that offers promising solutions to the aforementioned challenges, mainly due to its rich composition of proteins, vitamins, minerals, and bioactive compounds such as β-carotene and phycocyanin. These compounds confer various health benefits, including antioxidant, anticancer, anti-diabetic, antimicrobial, and anti-inflammatory properties, which make Spirulina a valuable dietary and therapeutic supplement. Essential fatty acids and its rapid growth rate also makes Spirulina a potential source of biodiesel for energy related applications. Additionally, Spirulina's high porosity and variable functional groups endow it with remarkable biosorption properties for soil and wastewater remediation applications. The chemical structure and unique properties of Spirulina have been utilized to produce biotemplates for nanomaterials as well as the fabrication of functional composites for various applications. Thus, in this review, we have highlighted the broad potentials of Spirulina in diverse applications, emphasizing its eco-friendliness, economic viability, challenges, and the prospects of its biomass for sustainable, nutraceutical, therapeutic, energy related, and environmental applications.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"51"},"PeriodicalIF":4.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of agri-food crucifer vegetables waste for food, functional food and nutraceuticals applications. 十字花科蔬菜废弃物在食品、功能食品和营养药品中的应用。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-06-01 DOI: 10.1186/s40643-025-00895-4

Lereen Khaled, Nada M Ali, Reem Nader, Ninon Rolet, Elizabeth S Sadek, Mohamed A Farag, Tamer Shoeib

{"title":"Valorization of agri-food crucifer vegetables waste for food, functional food and nutraceuticals applications.","authors":"Lereen Khaled, Nada M Ali, Reem Nader, Ninon Rolet, Elizabeth S Sadek, Mohamed A Farag, Tamer Shoeib","doi":"10.1186/s40643-025-00895-4","DOIUrl":"10.1186/s40643-025-00895-4","url":null,"abstract":"Valorization of food byproducts, especially fruits and vegetables, has recently attracted considerable attention, mostly due to their high wastage rates. Exploitation of these byproducts, including the non-edible parts of crucifer vegetables, may provide value-added opportunities in the food, functional food, and nutraceutical industries as well as in non-food applications such as therapeutics, biofuels, and paper pulp production. This review focuses on the state-of-the-art valorization practices of crucifer vegetable agro-food wastes including those of broccoli, cauliflower, cabbage, kale, Brussels sprouts, collards, watercress and radish constituting the main cultivated crucifer vegetables worldwide and suggests potential novel uses through upcycling. A detailed comparative phytochemical composition of crucifer vegetable waste products as potential sources of raw materials in promising applications including the production of food enhancers, and antioxidants is presented. Different extraction techniques combining downstream and white biotechnology processes for the optimum utilization of such agro-food waste are discussed. The valorization of cruciferous vegetables by-products is shown to be economical, sustainable and a viable approach to unlock novel applications across diverse industries. To fully maximize the potential of these underutilized resources and promote an ecological bioeconomy, more research and development into extraction methods and upcycling techniques is needed.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"50"},"PeriodicalIF":4.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the sequence of extracting Pinus radiata bark to maximize the total yield of extractives-towards the development of a bark-based biorefinery. 确定辐射松树皮的提取顺序，以最大限度地提高提取物的总收率——朝着以树皮为基础的生物炼制的方向发展。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-31 DOI: 10.1186/s40643-025-00896-3

Sumanth Ranganathan, Sylke H Campion, Reid A Dale, Queenie L Tanjay, Rachel H Murray, Anna de Lena, Michael Robertson, Armin Thumm, Mark West

{"title":"Determining the sequence of extracting Pinus radiata bark to maximize the total yield of extractives-towards the development of a bark-based biorefinery.","authors":"Sumanth Ranganathan, Sylke H Campion, Reid A Dale, Queenie L Tanjay, Rachel H Murray, Anna de Lena, Michael Robertson, Armin Thumm, Mark West","doi":"10.1186/s40643-025-00896-3","DOIUrl":"10.1186/s40643-025-00896-3","url":null,"abstract":"Pinus radiata is the dominant tree species in exotic plantation forestry of New Zealand producing timber for construction and pulp and paper. Additionally, the processing yields large amounts of bark as a byproduct that is either left at the harvest site or used for landscaping. P. radiata bark is rich in biochemical extractives containing polyphenols and waxes on sequential extraction with hydrophilic and lipophilic solvents, respectively. Previous studies have exclusively focussed on the effect of parameters such as solvent type, bark to solvent ratio, and extraction time on the yield of extractives. However, two parameters were always maintained constant: solvent order (lipophilic to hydrophobic) and particle size. This work investigated the the combined impact of these two parameters on total yield and product quality by using two solvents- water and hexane. Total extractives were highest when water was used first (11.74% and 9.45%) compared to hexane (10.53% and 6.53%). The individual yields of hexane extractives were in the range of 2.25-2.9% while those of water were 4.30-9.24%. Chemical analyses of the extracts and residues showed no qualitative differences, indicating the order in which bark is extracted does not alter the extract composition. Moreover, the results have successfully established that extracting bark with water first followed by hexane will increase the total yield of extractives and increasing particle size decreases the total yield of the sequential extraction.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"49"},"PeriodicalIF":4.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable and green extraction of citrus peel essential oil using intermittent solvent-free microwave technology. 间歇式无溶剂微波技术可持续绿色提取柑橘皮精油。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-29 DOI: 10.1186/s40643-025-00885-6

Yuhao Xie, Longdi Zhang, Wenting Wu, Jiahao Xie, Boliang Gao, Yiwen Xiao, Du Zhu

{"title":"Sustainable and green extraction of citrus peel essential oil using intermittent solvent-free microwave technology.","authors":"Yuhao Xie, Longdi Zhang, Wenting Wu, Jiahao Xie, Boliang Gao, Yiwen Xiao, Du Zhu","doi":"10.1186/s40643-025-00885-6","DOIUrl":"10.1186/s40643-025-00885-6","url":null,"abstract":"In the present study, a novel technology called intermittent solvent-free microwave extraction (ISFME), which integrates intermittent microwave treatment with solvent-free extraction, was proposed. Taking Nanfeng citrus peel as the research object, the extraction efficiency and composition of its essential oil were evaluated and compared with conventional hydrodistillation (HD) and solvent-free microwave extraction (SFME). Results from single-factor experiments and response surface method optimization indicate that the optimal parameters for SFME are a power of 660 W, a duration of 20 min, and a water content of 60%, yielding an essential oil output of 3.47%. In contrast, the optimal parameters for ISFME, determined through single-factor experiments, are a moisture content of 50%, a power setting of 380 W, a reaction time of 6 min, a 15 min interval, and 4 reaction cycles, resulting in an essential oil yield of 3.51%. Notably, the extraction efficiencies of ISFME and SFME are significantly superior to that of HD, which requires 240 min and yields only 3.28% essential oil. Furthermore, ISFME demonstrates comparable performance to SFME in terms of reaction time, extraction rate, and oil quality, while operating at a substantially lower power requirement of 380 W compared to the 660 W needed for SFME. Additionally, the carbon dioxide emissions and energy consumption associated with ISFME are markedly lower than those of traditional methods, amounting to only one-third of the energy consumption of SFME. These findings underscore that ISFME not only enhances the efficiency and quality of essential oil extraction but also minimizes environmental impact, thereby highlighting its significant advantages in terms of sustainability and ecological responsibility.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"48"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22. 离活性中心不同距离的突变对漆酶13B22活性和稳定性的影响。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-27 DOI: 10.1186/s40643-025-00893-6

Ruohan Zhang, Yuchen Wang, Xiaolu Wang, Huiying Luo, Yuan Wang, Bin Yao, Huoqing Huang, Jian Tian, Feifei Guan

{"title":"Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22.","authors":"Ruohan Zhang, Yuchen Wang, Xiaolu Wang, Huiying Luo, Yuan Wang, Bin Yao, Huoqing Huang, Jian Tian, Feifei Guan","doi":"10.1186/s40643-025-00893-6","DOIUrl":"10.1186/s40643-025-00893-6","url":null,"abstract":"Laccases with high catalytic efficiency and high thermostability can drive a broader application scope. However, the structural distribution of key amino acids capable of significantly influencing the performance of laccases has not been explored in depth. Thirty laccase 13B22 mutants with changes in amino acids at distances of 5 Å (first shell), 5-8 Å (second shell), and 8-12 Å (third shell) from the active center were validated experimentally with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as substrate. Twelve of these mutants (first shell, 1; second shell, 4; third shell, 7) showed higher catalytic efficiency than the wild-type enzyme. Mutants D511E and I88L-D511E showed 5.36- and 10.58-fold increases in kcat/Km, respectively, with increases in optimal temperature of 15 °C and optimal pH from 7.0 to 8.0. Furthermore, both mutants exhibited greater thermostability compared to the wild-type, with increases of 3.33 °C and 5.06 °C in Tm and decreases of 0.39 J and 0.59 J in total structure energy, respectively. The D511E mutation resides in the third shell, while I88L is in the second shell, and their performance enhancements were attributed to alterations in the rigidity or flexibility of specific protein structural domains. Both mutants showed enhanced degradation efficiency for benzo[a]pyrene and zearalenone. These findings highlight the importance of the residues located far from the active center in the function of laccase (second shell and third shell), suggesting broader implications for enzyme optimization and biotechnological applications.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"47"},"PeriodicalIF":4.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum. 超临界二氧化碳萃取谷氨酸棒状杆菌虾青素的研究。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-26 DOI: 10.1186/s40643-025-00882-9

Jan Seeger, Maximilian Zäh, Volker F Wendisch, Christoph Brandenbusch, Nadja A Henke

{"title":"Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum.","authors":"Jan Seeger, Maximilian Zäh, Volker F Wendisch, Christoph Brandenbusch, Nadja A Henke","doi":"10.1186/s40643-025-00882-9","DOIUrl":"10.1186/s40643-025-00882-9","url":null,"abstract":"Astaxanthin, a red carotenoid with potent antioxidant properties, holds significant value in the feed, cosmetics, and nutraceutical industries. While traditionally sourced from microalgae, Corynebacterium glutamicum, a well-established industrial microorganism, has been engineered to serve as an efficient host for astaxanthin production. As astaxanthin integrates into the cellular membrane, effective extraction methods are essential to access this valuable compound. In this study, a sustainable batch extraction process using supercritical carbon dioxide (scCO₂) as a green solvent was developed. The effects of cosolvent concentration (0-9% (w/w)), temperature (50-75 °C), and pressure (450-650 bar) were investigated with regard to the extraction yield. An optimized extraction was achieved with 9% (w/w) ethanol as a cosolvent, at 68 °C and 550 bar, allowing the extraction of 67.5 ± 3.7% of the cellular astaxanthin within 0.5 h. Prolonging the extraction time further increased the recovery to 93.3%, which is comparable to processes that have been established for the extraction of astaxanthin from microalgae and yeast. This approach provides a scalable and environmentally friendly solution for industrial astaxanthin recovery.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"46"},"PeriodicalIF":4.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii. 与C. carboxidivorans和C. beijerinkis混合营养共培养发酵c1气体的生物转化。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-26 DOI: 10.1186/s40643-025-00881-w

Marina Fernández-Delgado, Pedro Enrique Plaza, M Teresa García-Cubero, Susana Lucas, Mónica Coca, Juan Carlos López-Linares

{"title":"Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii.","authors":"Marina Fernández-Delgado, Pedro Enrique Plaza, M Teresa García-Cubero, Susana Lucas, Mónica Coca, Juan Carlos López-Linares","doi":"10.1186/s40643-025-00881-w","DOIUrl":"10.1186/s40643-025-00881-w","url":null,"abstract":"The development of newfangled bioprocess strategies for the capture of C1-gases (CO and CO2) and their bioconversion into valuable products is currently one of the main focuses of research in order to achieve a more resilient world. This work analyses the viability of the co-culture C. carboxidivorans and C. beijerinkii to produce bioproducts (bioalcohols and organic acids) in mixotrophic conditions. In this way, the bioconversion of C1 gases (CO and CO2), in the presence of Fe0, using mixotrophic co-culture fermentation by C. carboxidivorans and C. beijerinkii, was evaluated, analyzing the influence of the ratio between both microorganisms, the pH, and the presence of Fe0. As a result, up to 7 g/L of butanol were achieved at pH 7, 12.5 g/L Fe0, and using a 1:1 ratio of C. carboxidivorans: C. beijerinkii, also improving the production of ethanol, acetic acid, and butyric acid as compared to individual culture fermentations. Finally, the operation in a bioreactor, comparing discontinuous and continuous gas feeding operation modes, was also studied, with better C1-gases utilization and overall fermentation efficiency (7 vs 4.6 g/L butanol) in continuous gas operation mode.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"45"},"PeriodicalIF":4.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing Pleurotus eryngii polysaccharides: gamma irradiation boosts antidiabetic and antioxidant potential. 活化杏鲍菇多糖：伽马辐射增强抗糖尿病和抗氧化潜能。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-23 DOI: 10.1186/s40643-025-00854-z

Eman H F Abd El-Zaher, Ehab M Tousson, Azza A Mostafa, Enas M El-Gaar, Galal Yahya, Yehia A-G Mahmoud

{"title":"Revitalizing Pleurotus eryngii polysaccharides: gamma irradiation boosts antidiabetic and antioxidant potential.","authors":"Eman H F Abd El-Zaher, Ehab M Tousson, Azza A Mostafa, Enas M El-Gaar, Galal Yahya, Yehia A-G Mahmoud","doi":"10.1186/s40643-025-00854-z","DOIUrl":"10.1186/s40643-025-00854-z","url":null,"abstract":"Polysaccharides derived from Pleurotus eryngii possess various bioactive properties, including antioxidant, antidiabetic, anti-inflammatory, and immunomodulatory effects. In this study, polysaccharides were extracted from P. eryngii fruiting bodies and exposed to gamma irradiation at doses of 50 and 100 kGy, with a dose rate of 5 kGy/h. The surface morphology of the polysaccharide irradiated at 100 kGy exhibited numerous pores and a smaller flake structure compared to those irradiated at 50 kGy and the non-irradiated sample. 1H and 13C NMR spectra of all samples indicated that both irradiated and non-irradiated polysaccharides exhibited α- and β-configurations, with signals corresponding to C1-C5 clearly observed. HPLC analysis of the polysaccharides revealed that glucose (75.23%), galactose (4.96%), glucuronic acid (1.38%), ribose (0.94%), rhamnose (2.35%), and mannose (3.87%) are the main components. All polysaccharides demonstrated antioxidant activity, which increased with concentration. Both non-irradiated and irradiated polysaccharides exhibited antidiabetic effects, significantly reducing blood glucose levels, and restoring insulin level with superiority of irradiated polysaccharides. Additionally, they significantly elevated body weight, slightly reduced MDA levels, and markedly enhanced catalase activity in treated rats compared to diabetic controls. The antidiabetic effects of the polysaccharides were further confirmed by histopathological examination of the pancreas and liver sections from polysaccharide-treated diabetic rats. This suggests that irradiation, by reducing the molecular weight of polysaccharides, enhances their bioavailability and efficacy in modulating glucose metabolism.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"44"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in microbial composition during flue-cured tobacco aging and their effects on chemical composition: a review. 烤烟老化过程中微生物组成的变化及其对化学成分的影响

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2025-05-21 DOI: 10.1186/s40643-025-00883-8

Yangyang Yu, Yongfeng Yang, JiaTao Jia, Hang Zhou, Yao Qiu, Mengyao Sun, Hongli Chen

{"title":"Changes in microbial composition during flue-cured tobacco aging and their effects on chemical composition: a review.","authors":"Yangyang Yu, Yongfeng Yang, JiaTao Jia, Hang Zhou, Yao Qiu, Mengyao Sun, Hongli Chen","doi":"10.1186/s40643-025-00883-8","DOIUrl":"10.1186/s40643-025-00883-8","url":null,"abstract":"The aging is a crucial stage in tobacco processing, which contributes to the reduction of impurities and irritation, and the stabilization of the internal chemical composition of the leaves. However, it usually takes a long time (2-3 years) for the nature aging process of tobacco (20 °C-30 °C, relative humidity of 65-75%), which seriously affects the processing efficiency of tobacco. Microorganisms play an important role in the change of chemical composition and characteristic aromatic substances of tobacco. Acinetobacter, Sphingomonas Aspergillus, Bacilli, and Pseudonocardia is the main microorganism in the aging process of tobacco, which increasing the aromatic substances (such as alcohols, ketones, and esters) by the action of the enzymes and metabolites, and degrade the harmful components (such as alkaloid, nicotine and nitrosamines in tobacco). This review systematically summarizes recent advancements in understanding the primary microbial composition and the changes in chemical composition during tobacco aging. This knowledge is helpful for screening functional strains, and control the process of tobacco aging by the inoculation of these strains.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"43"},"PeriodicalIF":4.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0