Bioresources and Bioprocessing最新文献

Precise regulating the specific oxygen consumption rate to strengthen the CoQ₁₀ biosynthesis by Rhodobater sphaeroides. 精确调节特定耗氧量，加强水螅的 CoQ10 生物合成。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-11-05 DOI: 10.1186/s40643-024-00813-0

Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang

{"title":"Precise regulating the specific oxygen consumption rate to strengthen the CoQ10 biosynthesis by Rhodobater sphaeroides.","authors":"Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang","doi":"10.1186/s40643-024-00813-0","DOIUrl":"10.1186/s40643-024-00813-0","url":null,"abstract":"Coenzyme Q10 (CoQ10) is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides (R. sphaeroides). Oxygen supply was identified as a bottleneck for improving CoQ10 yield in R. sphaeroides. In this study, a precise regulation strategy based on dielectric spectroscopy (DS) was applied to further improve CoQ10 biosynthesis by R. sphaeroide. First, a quantitative response model among viable cells, cell morphology, and oxygen uptake rate (OUR) was established. DS could be used to detect viable R. sphaeroides cells, and the relationship among cell morphology, CoQ10 biosynthesis, and OUR was found to be significant. Based on this model, the online specific oxygen consumption rate (QO2) control strategy was successfully applied to the CoQ10 fermentation process. QO2 controlled at 0.07 ± 0.01 × 10- 7mmol/cell/h was most favorable for CoQ10 biosynthesis, resulting in a 28.3% increase in CoQ10 production. Based on the multi-parameters analysis and online QO2 control, a precise online nutrient feeding strategy was established using conductivity detected by DS. CoQ10 production was improved by 35%, reaching 3384 mg/L in 50 L bioreactors. This online control strategy would be effectively applied for improving industrial CoQ10 production, and the precise fermentation control strategy could also be applied to other fermentation process.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"106"},"PeriodicalIF":4.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomics-guided optimization of vitamins to enhance erythromycin yield in saccharopolyspora erythraea. 转录组学指导下的维生素优化，提高囊孢菌红霉素产量。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-11-01 DOI: 10.1186/s40643-024-00817-w

Xiang Ke, Xing Jiang, Shuohan Wang, Xiwei Tian, Ju Chu

{"title":"Transcriptomics-guided optimization of vitamins to enhance erythromycin yield in saccharopolyspora erythraea.","authors":"Xiang Ke, Xing Jiang, Shuohan Wang, Xiwei Tian, Ju Chu","doi":"10.1186/s40643-024-00817-w","DOIUrl":"10.1186/s40643-024-00817-w","url":null,"abstract":"Comparative transcriptomics uncovered distinct expression patterns of genes associated with cofactor and vitamin metabolism in the high-yielding mutant strain Saccharopolyspora erythraea HL3168 E3, as compared to the wild-type NRRL 2338. An in-depth analysis was conducted on the effects of nine vitamins, and it was determined that thiamine pyrophosphate (TPP), vitamin B2, vitamin B6, vitamin B9, vitamin B12, and hemin are key enhancers in erythromycin production in E3, increasing the erythromycin titer by 7.96-12.66%. Then, the Plackett-Burman design and the path of steepest ascent were applied to further optimize the vitamin combination for maximum production efficiency, enhancing the erythromycin titer in shake flasks by 39.2%. Otherwise, targeted metabolomics and metabolic flux analysis illuminated how vitamin supplementation modulates the central carbon metabolism with notable effects on the TCA cycle and methionine synthesis to augment the provision of energy and precursors essential for erythromycin synthesis. This work highlights the capacity for precise vitamin supplementation to refine metabolic pathways, thereby boosting erythromycin production, and provides valuable directions for application on an industrial scale.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"105"},"PeriodicalIF":4.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cosmetic efficacy of lychee seed fermentation liquid. 荔枝籽发酵液的美容功效评估。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-31 DOI: 10.1186/s40643-024-00818-9

Yaqian Yan, Hao Fu, Yuling Tang, Tiantian Huang, Xun Zou, Ning Su, Dongdong Wang, Changtao Wang, Meng Li

{"title":"Evaluation of cosmetic efficacy of lychee seed fermentation liquid.","authors":"Yaqian Yan, Hao Fu, Yuling Tang, Tiantian Huang, Xun Zou, Ning Su, Dongdong Wang, Changtao Wang, Meng Li","doi":"10.1186/s40643-024-00818-9","DOIUrl":"10.1186/s40643-024-00818-9","url":null,"abstract":"Background: Lychee seeds were fermented by three kinds of bacteria (Lactobacillus plantarum, Saccharomyces cerevillus and ganoderma lucidum mycelium), and two effective strains were selected by two indexes of activity content and antioxidant, so as to further verify whether lychee seeds have waste multiplication effect and can protect cells damaged by oxidation from anti-inflammatory, anti-aging and safety.Results: The contents of polyphenols, flavonoids and proteins in the solution fermented by Ganoderma lucidum mycelium did not increase, thus affecting the antioxidant capacity of the solution was far less than that of the water extract. The active content of the other two fermentation solutions was higher than that of the water extract, and the ability of scavenging free radicals of the two solutions increased with the increase of the volume fraction. At the cellular level, the two fermentation solutions showed repair effects on UVA-induced damaged cells. The contents of type I collagen (COL-1), total antioxidant capacity and ELN were increased, the contents of reactive oxygen species and MDA were decreased, and the expressions of inflammatory factors IL-6, TNF-a, iNOS and COX-2 were decreased in HaCaT cells. From the gene level, the mRNA contents of IL-6, TNF-a, Caspase-3, Caspase-9, Bax and Bcl were significantly decreased. The test of chick embryo chorioallantoic membrane (HTET CAM) showed that there was no bleeding and litchi seed fermentation liquid was not irritating.Conclusions: Therefore, two kinds of litchi seed fermentation can be used as natural plant raw materials for cosmetics, and have strong antioxidant, anti-inflammatory and anti-aging functions on skin, and also have good human safety.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"104"},"PeriodicalIF":4.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli. 利用工程大肠杆菌，在计算指导下发现用于生产月桂基葡糖苷的 UDP-糖基转移酶。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-26 DOI: 10.1186/s40643-024-00820-1

Kasimaporn Promubon, Kritsada Tathiya, Aussara Panya, Wasu Pathom-Aree, Pachara Sattayawat

{"title":"Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli.","authors":"Kasimaporn Promubon, Kritsada Tathiya, Aussara Panya, Wasu Pathom-Aree, Pachara Sattayawat","doi":"10.1186/s40643-024-00820-1","DOIUrl":"10.1186/s40643-024-00820-1","url":null,"abstract":"Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial for developing microbial cell factories for non-natural products. Here, we developed a computational workflow to identify C12 alcohol-active UDP-glycosyltransferases. The workflow involved three steps: (1) assembling initial candidates of putative UDP-glycosyltransferases, (2) refining selection by examining conserved regions, and (3) 3D structure prediction and molecular docking. Genomic sequences from Candida, Pichia, Rhizopus, and Thermotoga, known for lauryl glucoside synthesis via whole-cell biocatalysis, were screened. Out of 240 predicted glycosyltransferases, 8 candidates annotated as glycosyltransferases were selected after filtering out those with signal peptides and identifying conserved UDP-glycosyltransferase regions. These proteins underwent 3D structure prediction and molecular docking with 1-dodecanol. RO3G, a candidate from Rhizopus delemar RA 99-880 with a relatively high ChemPLP fitness score, was selected and expressed in Escherichia coli BL21 (DE3). It was further characterized using a feeding experiment with 1-dodecanol. Results confirmed that the RO3G-expressing strain could convert 1-dodecanol to lauryl glucoside, as quantified by HPLC and identified by targeted LC-MS. Monitoring the growth and fermentation profiles of the engineered strain revealed that RO3G expression did not affect cell growth. Interestingly, acetate, a major fermentation product, was reduced in the RO3G-expressing strain compared to the GFP-expressing strain, suggesting a redirection of flux from acetate to other pathways. Overall, this work presents a successful workflow for discovering UDP-glycosyltransferase enzymes with confirmed activity toward 1-dodecanol for lauryl glucoside production.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"103"},"PeriodicalIF":4.3,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of metabolites from the gut microbiota in hypertension via network pharmacology and molecular docking. 通过网络药理学和分子对接鉴定高血压肠道微生物群的代谢物。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-21 DOI: 10.1186/s40643-024-00815-y

Wenjie Zhang, Yinming Zhang, Jun Li, Jiawei Tang, Ji Wu, Zicong Xie, Xuanchun Huang, Shiyi Tao, Tiantian Xue

{"title":"Identification of metabolites from the gut microbiota in hypertension via network pharmacology and molecular docking.","authors":"Wenjie Zhang, Yinming Zhang, Jun Li, Jiawei Tang, Ji Wu, Zicong Xie, Xuanchun Huang, Shiyi Tao, Tiantian Xue","doi":"10.1186/s40643-024-00815-y","DOIUrl":"10.1186/s40643-024-00815-y","url":null,"abstract":"Hypertension is the most prevalent cardiovascular disease, affecting one-third of adults. All antihypertensive drugs have potential side effects. Gut metabolites influence hypertension. The objective of this study was to identify antihypertensive gut metabolites through network pharmacology and molecular docking techniques and to validate their antihypertensive mechanisms via in vitro experiments. A total of 10 core antihypertensive targets and 18 gut metabolites that act on hypertension were identified. Four groups of protein metabolites, namely, CXCL8-baicalein, CXCL8-baicalin, CYP1A1-urolithin A, and PTGS2-equol, which have binding energies of - 7.7, - 8.5, - 7.2, and - 8.8 kcal-mol-1, respectively, were found to have relatively high affinities. Based on its drug-likeness properties in silico and toxicological properties, equol was identified as a potential antihypertensive metabolite. On the basis of the results of network pharmacology and molecular docking, equol may exert antihypertensive effects by regulating the IL-17 signaling pathway and PTGS2. A phenylephrine-induced H9c2 cell model was subsequently utilized to verify that equol inhibits cell hypertrophy (P < 0.05) by inhibiting the IL-17 signaling pathway and PTGS2 (P < 0.05). This study demonstrated that equol has the potential to be developed as a novel therapeutic agent for the treatment of hypertension.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"102"},"PeriodicalIF":4.3,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Microwave-ultrasonic assisted extraction of lignin to synthesize new nano micellar organometallic surfactants for refining oily wastewater. 撤回声明：微波-超声波辅助萃取木质素以合成用于含油废水精炼的新型纳米胶束有机金属表面活性剂。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-18 DOI: 10.1186/s40643-024-00819-8

M H Alhalafi, S A Rizk, E S Al-Malki, A M Algohary

引用次数: 0

Engineering a high-sugar tolerant strain of Saccharomyces cerevisiae for efficient trehalose production using a cell surface display approach. 利用细胞表面展示法培育高糖耐受性酿酒酵母菌株，以高效生产曲哈糖。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-18 DOI: 10.1186/s40643-024-00816-x

Kan Tulsook, Piyada Bussadee, Jantima Arnthong, Wuttichai Mhuantong, Panida U-Thai, Srisakul Trakarnpaiboon, Verawat Champreda, Surisa Suwannarangsee

{"title":"Engineering a high-sugar tolerant strain of Saccharomyces cerevisiae for efficient trehalose production using a cell surface display approach.","authors":"Kan Tulsook, Piyada Bussadee, Jantima Arnthong, Wuttichai Mhuantong, Panida U-Thai, Srisakul Trakarnpaiboon, Verawat Champreda, Surisa Suwannarangsee","doi":"10.1186/s40643-024-00816-x","DOIUrl":"10.1186/s40643-024-00816-x","url":null,"abstract":"Trehalose production via a one-step enzymatic route using trehalose synthase (TreS) holds significant promise for industrial-scale applications due to its simplicity and utilization of low-cost substrates. However, the development of a robust whole-cell biocatalyst expressing TreS remains crucial for enabling practical and economically viable production. In this study, a high-sugar tolerant strain of S. cerevisiae was screened and employed as a host cell for the cell surface display of TreS from Acidiplasma aeolicum. The resultant strain, S. cerevisiae I3A, exhibited remarkable surface displayed TreS activity of 3358 U/g CDW and achieved approximately 64% trehalose yield (10.8 g/L/h productivity) from maltose. Interestingly, no glucose by-product was observed during trehalose production. The S. cerevisiae I3A cells exhibited reusability for up to 12 cycles leading to potential cost reduction of trehalose products. Therefore, our study demonstrated the development of a high-sugar tolerant S. cerevisiae strain expressing TreS on its surface as a whole-cell biocatalyst for efficient and economical trehalose production with potential applications in the food and pharmaceutical industries.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"101"},"PeriodicalIF":4.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient synthesis of the chiral ACE inhibitor intermediate (R)-2-hydroxy-4-phenylbutyrate ethyl ester via engineered bi-enzyme coupled systems. 通过工程化双酶偶联系统高效合成手性 ACE 抑制剂中间体 (R)-2- 羟基-4-苯基丁酸乙酯。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-15 DOI: 10.1186/s40643-024-00814-z

Yanmei Dai, Jinmei Wang, Zijuan Tao, Liangli Luo, Changshun Huang, Bo Liu, Hanbing Shi, Lan Tang, Zhimin Ou

{"title":"Highly efficient synthesis of the chiral ACE inhibitor intermediate (R)-2-hydroxy-4-phenylbutyrate ethyl ester via engineered bi-enzyme coupled systems.","authors":"Yanmei Dai, Jinmei Wang, Zijuan Tao, Liangli Luo, Changshun Huang, Bo Liu, Hanbing Shi, Lan Tang, Zhimin Ou","doi":"10.1186/s40643-024-00814-z","DOIUrl":"https://doi.org/10.1186/s40643-024-00814-z","url":null,"abstract":"(R)-2-Hydroxy-4-phenylbutyric acid ethyl ester ((R)-HPBE) is an essential chiral intermediate in the synthesis of angiotensin-converting enzyme (ACE) inhibitors. Its production involves the highly selective asymmetric reduction of ethyl 2-oxo-4-phenylbutyrate (OPBE), catalyzed by carbonyl reductase (CpCR), with efficient cofactor regeneration playing a crucial role. In this study, an in-situ coenzyme regeneration system was developed by coupling carbonyl reductase (CpCR) with glucose dehydrogenase (GDH), resulting in the construction of five recombinant strains capable of NADPH regeneration. Among these, the recombinant strain E. coli BL21-pETDuet-1-GDH-L-CpCR, where CpCR is fused to the C-terminus of GDH, demonstrated the highest catalytic activity. This strain exhibited an enzyme activity of 69.78 U/mg and achieved a conversion rate of 98.3%, with an enantiomeric excess (ee) of 99.9% during the conversion of 30 mM OPBE to (R)-HPBE. High-density fermentation further enhanced enzyme yield, achieving an enzyme activity of 1960 U/mL in the fermentation broth, which is 16.2 times higher than the volumetric activity obtained from shake flask fermentation. Additionally, the implementation of a substrate feeding strategy enabled continuous processing, allowing the strain to efficiently convert a final OPBE concentration of 920 mM, producing 912 mM of (R)-HPBE. These findings highlight the system's improved catalytic efficiency, stability, and scalability, making it highly suitable for industrial-scale biocatalytic production.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"99"},"PeriodicalIF":4.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate). 更正：对快速生长的纳氏弧菌进行生物合成聚（3-羟基丁酸-乳酸）工程学研究。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-14 DOI: 10.1186/s40643-024-00811-2

Xinye Sun, Yanzhe Shang, Binghao Zhang, Pengye Guo, Yuanchan Luo, Hui Wu

引用次数: 0

Correction: Enhancement of lactate fraction in poly(lactate-co-3-hydroxybutyrate) biosynthesized by metabolically engineered E. coli. 更正：提高代谢工程大肠杆菌生物合成的聚（乳酸-3-羟基丁酸）中的乳酸含量。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-10-12 DOI: 10.1186/s40643-024-00810-3

Binghao Zhang, Pengye Guo, Xinye Sun, Yanzhe Shang, Yuanchan Luo, Hui Wu

引用次数: 0