Bioresources and Bioprocessing最新文献

{"title":"The driving role of extracellular polymeric substances in the bioelectrical conversion of petroleum hydrocarbons by rhizosphere microbial fuel cells: bioelectricity production, substrate bioconversion, microbial function and their network correlation.","authors":"Lanmei Zhao, Yuru Wen, Lyu Can, Long Meng, Jian Liu, Dong Zhao","doi":"10.1186/s40643-025-00922-4","DOIUrl":"https://doi.org/10.1186/s40643-025-00922-4","url":null,"abstract":"<p><p>Extracellular polymeric substances (EPS), are crucial components of biofilms that drive the bioelectrical conversion of petroleum hydrocarbons (PHCs), but their role has not been adequately addressed. This research explores the driving role of EPS in bioelectrical PHC conversion by rhizosphere microbial fuel cells (MFCs). We found that current density, output voltage, coulombic efficiency, power density, current stabilization time, metabolite volatile fatty acid (VFA) production and PHC biodegradation ratio initially increased and then decreased with rising initial EPS level (0-128 mg·g^- 1), peaking at 64 ± 1 mA·m^- 2, 8.04 ± 0.16 V, 60.9 ± 1.2%, 129 ± 3 mW·m^- 2, 23 ± 1 days, 1.77 ± 0.04 g·kg^- 1 and 66.7 ± 1.5%, respectively. Fluorescence intensity of proteins having tyrosine-tryptophan demonstrated a continuous enhancement, consistent with increased biofilm thickness. Within an appropriate range of initial EPS levels (0-64 mg·g⁻¹), bioelectricity generation and PHC bioconversion enhanced as the EPS content rose in mature biofilms. However, excessive EPS addition could increase biofilm thickness to 0.48 mm, which in turn reduced biofilm activity and overall system performance. The abundances of electrochemically active and PHC-degrading bacteria presented an initial increase followed by a subsequent decrease as the initial EPS level rose, highlighting that EPS at the optimal level enriched and activated these functional bacteria. The positive correlations between the relative abundances of these bacteria and various metrics of bioelectricity generation and PHC bioconversion underscored the critical role of EPS in shaping microbial community structure and enhancing electron transfer efficiency through biofilm formation and stabilization. These findings not only provide a critical theoretical foundation and novel ideas to promote the conversion of PHC into renewable bioenergy but also highlight the potential scalability and environmental benefits of this technology in the field of clean remediation of PHC-polluted soils and recovery of bioenergy. Integrating EPS-driven MFCs with other renewable energy technologies will offer promising opportunities to develop hybrid systems that generate clean energy while mitigating environmental pollution. Furthermore, this approach also has the potential as biosensors for the real-time detection of PHCs, thus contributing to broadening its application in environmental monitoring.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"79"},"PeriodicalIF":4.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar microbiota confers deeper color of fermented cigar wrapper under additional fermented bacteria.","authors":"Qian Wu, Xuanshuang Duan, Dapeng Tang, Rui Wang, Fuqiang Li, Jun Tan, Honghua Chen, Shixue Zheng","doi":"10.1186/s40643-025-00921-5","DOIUrl":"https://doi.org/10.1186/s40643-025-00921-5","url":null,"abstract":"<p><p>Microorganisms play an indispensable role in the fermentation of cigar tobacco such as improvement of flavor, aroma and quality. However, little is known about additional microorganism impact on shift of the color of cigar wrappers. Here, three bacterial strains that could alter the wrapper's color were isolated and screened from cigar tobacco leaves (CTLs) at different stages of fermentation. The three strains were identified to be Sphingomonas parapaucimobilis DS1, Kosakonia cowanii DS101, and Comamonas thiooxydans DS309 based on the genome sequences. The overall color difference (∆E) of CTLs was significantly deepened with the addition of those three strains under upscaled stack fermentation, respectively (p ≤ 0.05), showing the same fundamental quality of cigar wrapper. The ascorbic acid oxidase activity was significantly decreased and cigarillo polyphenol oxidase activity was significantly increased added with the three bacteria compared to the control (p ≤ 0.05). Moreover, the exogenous strains substantially altered the community structure of the cigar wrapper. In particular, the abundance of dominant Staphylococcus nepalensis significantly increased under addition of strain DS101 and DS309 except for strain DS1 as dominant species. In addition, non-targeted metabolomics analyses showed that the exogenous microbial addition treatments demonstrated substantial variations in a variety of metabolites, including a reduced expression in the pathways related to the manufacture of polyphenols and unsaturated fatty acids (p ≤ 0.05). In conclusion, addition of bacteria DS1, DS101, and DS309 enhanced oxidation of polyphenols to brown quinones and oxidation of unsaturated fatty acids, resulting in membrane lipid peroxidation and then deepened the color of cigar wrappers. It provides a novel strategy to improve the color of cigar wrappers by utilizing additional bacteria in cigar fermentation.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"78"},"PeriodicalIF":4.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using of transporter proteins to improve the uptake efficiency of hydrophobic compounds by Escherichia coli: a coordinated synthesis of START protein and P450scc system proteins to enhance cholesterol biotransformation.","authors":"Sofia V Zamalutdinova, Ludmila V Isaeva, Yaroslav V Faletrov, Nikolay N Eroshchenko, Alexey N Kirushin, Vadim N Tashlitsky, Mikhail A Rubtsov, Ludmila A Novikova","doi":"10.1186/s40643-025-00909-1","DOIUrl":"10.1186/s40643-025-00909-1","url":null,"abstract":"<p><p>Synthesis of steroid drugs is possible through biotechnological methods, however, the low efficiency of transporting steroid substrates across microbial membranes is the challenge in using microorganisms for their production. STARD1 and STARD3 proteins (members of the START domain family) work in some mammalian cells in conjunction with the steroidogenic P450scc enzyme system (cytochrome P450scc, adrenodoxin reductase, and adrenodoxin), transporting cholesterol to the mitochondria, where cytochrome P450scc converts it into pregnenolone. In this study, we investigated the effect of STARD1 and STARD3 on P450scc efficiency in E. coli expression models. The combination of the STARD3(216-444) or STARD1(53-285) protein bearing an N-terminal periplasm-targeting sequence, with the P450scc system reconstituted in E. coli, resulted in an increase of pregnenolone synthesis by 3.2- and 1.9-fold, respectively, on a laboratory scale. STARD3(216-444) showed higher levels of expression and a greater effect on the incorporation of sterols into cells and whole-cell biotransformation of cholesterol, compared to STARD1(53-285). This study proposes a fundamentally new approach to optimizing the functioning of the P450scc system in microbial cells, which uses the cholesterol transfer protein to increase the uptake efficiency of a poorly soluble steroid substrate by bacteria. The demand for steroid medications is increasing, and the use of specific carrier proteins could be a useful tool to enhance the efficiency of whole-cell biosynthesis of various steroid compounds.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"77"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Optimization of extraction process and solvent polarities to enhance the recovery of phytochemical compounds, nutritional content, and biofunctional properties of Mentha longifolia L. extracts.","authors":"Meryem Tourabi, Khaoula Faiz, Rachid Ezzouggari, Bouchra Louasté, Mohammed Merzouki, Musaab Dauelbait, Mohammed Bourhia, Khalid S Almaary, Farhan Siddique, Badiaa Lyoussi, Elhoussine Derwich","doi":"10.1186/s40643-025-00910-8","DOIUrl":"10.1186/s40643-025-00910-8","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"76"},"PeriodicalIF":4.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12274164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive expression of eukaryotic cytochrome P450 ferulate-5-hydroxylase in Escherichia coli for sustainable synthesis of antioxidant 5-hydroxyferulic acid.","authors":"Ping Sun, Yuan Tian, Luyi Wang, Pengcheng Chen, Dan Wu, Pu Zheng","doi":"10.1186/s40643-025-00919-z","DOIUrl":"10.1186/s40643-025-00919-z","url":null,"abstract":"<p><p>The eukaryotic cytochrome P450 ferulate-5-hydroxylase (F5H), a membrane-bound protein, plays a critical role in lignin synthesis involved in the biosynthesis of 5-Hydroxyferulic acid (5-HFA) from ferulic acid, with 5-HFA offering enhanced antioxidant properties. However, there is challenging to engineer recombinants for 5-HFA synthesis by expressing F5H in Escherichia coli. In this work, we co-expressed F5H derived from Arabidopsis thaliana (AtF5H) and NADPH-dependent cytochrome P450 reductase (CPR) in E. coli, and successfully synthesized ortho-hydroxylated ferulic acid. Simultaneously, by modifying the N-terminal regions of membrane proteins, we increased the concentration of product 5-HFA to 63.6 mg/L. Ferulic acid, utilized as the substrate, was extracted from discarded agricultural by-products, demonstrating a sustainable approach to valorizing agricultural waste. This work also advances the application of plant-derived membrane-bound proteins for the production of plant secondary metabolites in E. coli.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"75"},"PeriodicalIF":4.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrobium officinale-derived nanovesicles: a natural therapy for comprehensive regulation of angiogenesis, inflammation, and tissue repair to enhance skin wound healing.","authors":"Jin Tu, Luhua Xu, Yuqin Guo, Minzhi Zhang, Miao Gan, Xiuzhen Bao, Rongfeng Yang, Hanjiao Liu, Fengxia Lin","doi":"10.1186/s40643-025-00915-3","DOIUrl":"10.1186/s40643-025-00915-3","url":null,"abstract":"<p><p>Skin wound healing is a multifaceted biological process involving dynamic interactions among various cells and signaling molecules. Angiogenesis is a key component of this repair process. Dendrobium officinale, a traditional medicinal plant, has shown therapeutic promise, particularly through its bioactive nanovesicles. This study investigates the therapeutic potential of Dendrobium officinale-derived nanovesicles (DDNVs) in regulating angiogenesis, inflammation, and tissue repair, to promote enhanced skin wound healing. A full-thickness mouse skin wound model was used to evaluate the in vivo effects of DDNVs on wound closure, angiogenesis, and collagen remodeling. Histological staining (H&E and Masson's trichrome) and CD31 immunofluorescence were performed. In vitro, DDNVs were tested on Human umbilical vein endothelial cells(HUVECs) and Human keratinocyte cells (HaCaT) cells to assess cell proliferation, migration, and angiogenesis. Confocal microscopy was used to track cellular uptake. Activation of the Akt/eNOS pathway and expression of key genes related to inflammation and matrix remodeling were evaluated by Western blotting and qPCR. DDNVs significantly accelerated wound healing and promoted angiogenesis in vivo, as evidenced by enhanced CD31 expression and collagen remodeling. In vitro, DDNVs entered cells efficiently and stimulated HUVEC and HaCaT proliferation and migration. This was accompanied by activation of the Akt/eNOS signaling pathway, increased expression of eNOS and VEGFR-2, upregulation of extracellular matrix(ECM) components (Vimentin, Fibronectin, COL1A1), and suppression of inflammatory markers such as ICAM-1 and IL-1β. DDNVs exhibit strong potential to enhance skin wound healing by promoting angiogenesis, improving tissue repair, and modulating inflammation. These findings support the clinical development of DDNVs as a novel, plant-derived nanotherapeutic for chronic wound treatment and skin regeneration.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"74"},"PeriodicalIF":4.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of conjugated linoleic acid: current status and future perspectives.","authors":"Lu Lin, Mei-Li Sun, Kaifeng Wang, Jian Gao, Xiao-Jun Ji, Quanyu Zhao","doi":"10.1186/s40643-025-00911-7","DOIUrl":"10.1186/s40643-025-00911-7","url":null,"abstract":"<p><p>Conjugated linoleic acid (CLA) has received much attention for its beneficial physiological effects, particularly anticancer and metabolic control activity. However, traditional sources rely on extraction and chemical synthesis, both of which are not commercially viable. This article describes the research progress on sustainable alternative biosynthesis methods for the production of CLA, which is an attractive approach for commercial production because of its high stereoselectivity and convenient purification process. Here, we offer a comprehensive review of the current state and research progress in the biosynthesis of CLA, including production strains, biosynthesis mechanisms and improvements in the biosynthesis of CLA by genetic engineering. Finally, future research directions for improving the biosynthesis of CLA are defined in the light of recent advances, barriers and trends in this field.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"72"},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing polyphenols from pulp industry residues of juvenile eucalyptus wood: potential for adhesive applications.","authors":"Lucía Xavier, Rodrigo Coniglio, Fabián Bermúdez, Diego Passarella, Leonardo Clavijo","doi":"10.1186/s40643-025-00914-4","DOIUrl":"10.1186/s40643-025-00914-4","url":null,"abstract":"<p><p>Approximately 1-2% of the wood processed for pulp production is converted into sawdust and pinchips, which are commonly burned for energy in industries that already produce excess power. This material, juvenile wood only containing sapwood, poses challenges for its valorization. This study explores the extraction of polyphenols from Eucalyptus wood residues generated by a pulp industry in Uruguay, aiming to evaluate their suitability as partial replacements in phenol-formaldehyde (PF) adhesives. Using solid-liquid extraction with sodium hydroxide and sodium sulfite, the extractions conditions were optimized through an experimental design considering sodium hydroxide and sodium sulfite charges, along with temperature as variables. The extracts were characterized based on phenolic content, condensed tannins content, antioxidant activity, and Stiasny number, to assess the adhesive potential. Adhesives formulated with 10%, 20%, and 30% PF resin substitution demonstrated a progressive reduction in tensile shear strength, with the 10% substitution achieving acceptable strength. While higher substitution levels compromised adhesive performance, the results suggest that formulation adjustments may improve performance at moderate substitution ratios. Furthermore, the high antioxidant capacity of the extracts opens up potential for their use in other high-value applications where natural antioxidants are increasingly sought after as safer and more sustainable alternatives to synthetic additives.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"73"},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Changes in microbial composition during flue-cured tobacco aging and their effects on chemical composition: a review.","authors":"Yangyang Yu, Yongfeng Yang, Tao Jia, Hang Zhou, Yao Qiu, Mengyao Sun, Hongli Chen","doi":"10.1186/s40643-025-00913-5","DOIUrl":"10.1186/s40643-025-00913-5","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"71"},"PeriodicalIF":4.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antioxidant activity of brown seaweed Laminaria japonica by fermentation using isolated Bacillus subtilis.","authors":"Yueh-Hao Ronny Hung, Ya-Han Chang, Hsuan-Ju Lin, Li-Ho Chiang, Hong-Ting Victor Lin","doi":"10.1186/s40643-025-00912-6","DOIUrl":"10.1186/s40643-025-00912-6","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"69"},"PeriodicalIF":4.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}