Bioresources and Bioprocessing最新文献

{"title":"Integration of network pharmacology and experimental validation to explore the pharmacological mechanism of andrographolide against asthma.","authors":"Qian Yu, LiHong Zhu, XuChun Ding, YaFang Lou","doi":"10.1186/s40643-025-00869-6","DOIUrl":"https://doi.org/10.1186/s40643-025-00869-6","url":null,"abstract":"<p><p>Andrographolide (AG), one of the main active components of Andrographis paniculata (Burm.f.) Wall. ex Nees, has been proved to possess the pharmacological function of anti-inflammation in multiple disease including asthma. But the potential mechanism is still not clear. In this study, network pharmacology, molecular docking and experimental validation were utilized to explore the molecular mechanism of AG in the treatment of asthma. AG-related targets and asthma-related targets were screened by Swiss Target Prediction, DrugBank, STITCH, OMIM, Genecards and TTD databases. A protein-protein interaction (PPI) network was obtained through the STRING Database. The plug-in of \"Network Analyzer\" in Cytoscape 3.7.1 software was used to conduct the topological analysis. GO enrichment and KEGG pathway analysis were achieved by Metascape database and Bioinformatics platform. The target-pathway network was acquired by Cytoscape 3.7.1 software. The binding affinity between AG and the target genes was evaluated by Molecular docking with AutoDockTools 1.5.6. Flow cytometry was also used to verify the mechanism behind the treatment of asthma by AG, which was predicted in network pharmacology. In total, 38 targets were identified as potential targets of AG against asthma. The top 10 targets revealed by PPI are: IL-6, IL-1B, NFKB1, MMP9, CDK2, CREBBP, MAP2K1, JAK1, AR, PRKCA. GO and KEGG analysis showed that AG treatment of asthma mainly involved protein phosphorylation, peptidyl-serine phosphorylation, peptidyl-amino acid modification and other biological processes. The main signaling pathways are Th17 cell differentiation, JAK-STAT signaling pathway and PI3K-Akt signaling pathway. Molecular docking showed that AG has higher affinity with MMP9, PRKCA, JAK2, LTGAL and LRRK2. Flow cytometry showed that Th17 cell differentiation may be the potential target of AG in the treatment of asthma. This study successfully revealed the underlying target genes and mechanism involved in the treatment of asthma for AG, providing a reference and guidance for future mechanism research.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"30"},"PeriodicalIF":4.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the in vitro activity of extracted Euphorbia trigona via Supercritical Fluid Extraction against pathogenic yeasts, obesity, cancer, and its wound healing properties.","authors":"Abdulrahman S Bazaid, Naif K Binsaleh, Heba Barnawi, Bandar Alharbi, Ahmed Alsolami, Samy Selim, Soad K Al Jaouni, Amna A Saddiq, Magdah Ganash, Tarek M Abdelghany, Husam Qanash","doi":"10.1186/s40643-025-00855-y","DOIUrl":"10.1186/s40643-025-00855-y","url":null,"abstract":"<p><p>Natural products of plant origin are being explored as safe alternatives for illness management. Their extraction processes play a crucial role in determining their phytochemical and pharmacological properties. In this context, Euphorbia trigona was extracted using Supercritical Fluid Extraction with CO₂ (SFE-CO₂) at two operating temperatures: 20 °C and 40 °C. Phytochemical characterization was performed via HPLC, along with anti-yeast evaluation using the well diffusion method, anticancer assessment using the MTT assay, wound healing analysis via the scratch assay, and anti-obesity evaluation through the lipase assay of the E. trigona extract. The results indicated that SFE-CO₂ at 40 °C extracted a greater quantity (0.198 g) of E. trigona than SFE-CO₂ at 20 °C (0.156 g). Several compounds, such as rosmarinic acid, gallic acid, daidzein, ellagic acid, naringenin, and ferulic acid, were identified at high concentrations of 10,034.29, 1,800.33, 750.22, 748.11, 462.15, and 207.05 µg/mL, respectively, in the E. trigona extract obtained using SFE-CO₂ at 40 °C, compared to the extract obtained using SFE-CO₂ at 20 °C. High inhibition zones of 24 ± 1.5, 24 ± 0.5, and 23 ± 0.33 mm were recorded against C. albicans, C. tropicalis, and G. candidum, respectively, using the extract from SFE-CO₂ at 40 °C, compared to the inhibition zones of 24 ± 1.5, 24 ± 0.5, and 23 ± 0.33 mm obtained from the extract using SFE-CO₂ at 20 °C. Moreover, the extract from SFE-CO₂ at 40 °C exhibited lower MIC and MFC values against the tested yeasts compared to the efficacy of the extract from SFE-CO₂ at 20 °C. The ultrastructure of the examined yeasts was severely affected by the extract from SFE-CO₂ at 40 °C. A lower IC₅₀ (98.87 ± 1.26 µg/mL) was recorded for the extract from SFE-CO₂ at 40 °C compared to the IC₅₀ (333.87 ± 1.8 µg/mL) of the extract from SFE-CO₂ at 20 °C against cancer cells (A431). The wound closure level was 84.08% using the extract from SFE-CO₂ at 40 °C, while it was 71.27% using the extract from SFE-CO₂ at 20 °C. Lipase was inhibited by the extract obtained via SFE-CO₂ at 40 °C and 20 °C, with IC₅₀ values of 15.77 and 28.14 µg/mL, respectively. Molecular docking indicated that rosmarinic acid is a suitable inhibitor for the tested yeasts.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"28"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities.","authors":"Karnan Ramachandran, Senthil Bakthavatchalam, Shunmuga Vadivu Ramalingam, Ramachandran Vinayagam, Mukeshwaran Ramesh, Sukumaran Marimuthu, Zhi-Hong Wen, Chandramohan Govindasamy, Khalid M Almutairi, Yi-Hao Lo","doi":"10.1186/s40643-025-00868-7","DOIUrl":"10.1186/s40643-025-00868-7","url":null,"abstract":"<p><p>This study investigates the in silico anti-arboviral potential of zoochemicals derived from the methanolic extract of Charybdis natator shell, alongside their larvicidal efficacy against Aedes aegypti 4th instar larvae. Through GC-MS analysis, 27 zoochemicals were identified, demonstrating promising in silico activity against molecular antiviral targets: DENV2 protease (PDB: 6MO1) for anti-dengue, RNA polymerase (PDB: 5U04) for anti-Zika, and nsP2 protease (PDB: 3TRK) for anti-chikungunya. A strong positive correlation (r = 0.726-0.889) in binding affinities (kcal/mol) suggests a consistent inhibitory mechanism across these targets. Furthermore, PASS analysis indicates higher probabilities of activity (Pa) for insecticidal properties compared to antiviral efficacy, highlighting their dual potential as larvicidal agents and antiviral candidates. The methanolic extract of Charybdis natator shell exhibited potent larvicidal activity against Aedes aegypti (LC₅₀ = 81.001 µg/mL) in a dose-dependent manner (R² = 0.968). In silico analysis further elucidated its inhibitory action on key growth regulators of A. aegypti, underscoring its potential to disrupt larval development. These findings highlight the dual utility of C. natator shell extract in vector management and in mitigating the transmission of arboviral diseases such as Dengue, Zika, and Chikungunya. The extract's promise as an eco-friendly, cost-effective source for developing novel insecticidal and antiviral agents merits further exploration.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"29"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phycochemistry and pharmacological significance of filamentous cyanobacterium Spirulina sp.","authors":"Sanjana Sabat, Shuvasree Bej, Surendra Swain, Ajit Kumar Bishoyi, Chita Ranjan Sahoo, Goutam Sabat, Rabindra Nath Padhy","doi":"10.1186/s40643-025-00861-0","DOIUrl":"10.1186/s40643-025-00861-0","url":null,"abstract":"<p><p>The cyanobacterium, Spirulina sp. is a photosynthetic blue-green alga with essential nutrients, vitamins nucleic acids, proteins, carbohydrates, fatty acids and pigments carotenes; and phycocyanins are the significant components having immunomodulatory, anti-inflammatory properties, which are used in food and cosmetics industries. Spirulina sp. can play an important role in human and animal nutrition for potential health benefits due to their phycochemical and pharmaceutical significance. This study highlights antibacterial, antifungal, antiviral, antioxidant, nephroprotective, cardioprotective, anticancer, neuroprotective, anti-aging, anti-inflammatory, and immunomodulatory properties. It highlights anti-anemic, antidiabetic, probiotic, anti-malarial, anti-obesity and weight loss, anti-genotoxicity, anti-thrombic, radioprotective, and detoxifying effects of Spirulina sp. Pharmaceutical studies indicate it may improve heart health and add to the treatment of diabetes, obesity and weight loss. It can play a major role in protecting the environment by recycling wastewater and providing food for humans and animals. Spirulina sp. can supply ingredients for aquaculture and agricultural feeds, pigments, antioxidants, and essential omega-3 oils, among other human health and wellness products. The amino acid of Spirulina is among the greatest qualititavely of any plant, even higher than that of soybean. Furthermore, cyanobacterium Spirulina sp. could be a future antimicrobial drug agent.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"27"},"PeriodicalIF":4.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vinasses valorization into short-chain fatty acids: microbiome robustness against process variations.","authors":"Silvia Greses, Mercedes Llamas, Aboudi Kaoutar, Cristina González-Fernández","doi":"10.1186/s40643-025-00865-w","DOIUrl":"10.1186/s40643-025-00865-w","url":null,"abstract":"<p><p>The valorization of vinasses into short-chain fatty acids (SCFAs) via anaerobic fermentation (AF) is an emerging approach that remains under research. Given the diverse microbial metabolisms simultaneously occurring in AF, the control of operational parameters is essential to avoid process destabilization. To unravel their effect, the novelty of this investigation relied on the evaluation of the robustness of AF process against operational perturbation deliberately set (i.e. hydraulic retention time (HRT) and temperature increase). Regardless the applied perturbation, similar yields (0.5-0.6 g COD-SCFAs/g VS_in) were attained. However, the selected perturbations exerted an effect on microbiome development. Whereas the temperature increase mediated a 49.70% microbiome dissimilarity, only a 21.91% dissimilarity was caused by the HRT increase. Microbial analysis revealed Clostridiales, Prevotella and Megasphaera as key bacteria in vinasses degradation. The similar bioconversion obtained despite the different microbiomes developed after each perturbation suggested a functional redundancy highlighting the AF robustness. These findings evidenced AF as a feasible biotechnology to further valorize vinasse into SCFAs, demonstrating the process stability against common perturbations that might be encountered at industrial scale.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"26"},"PeriodicalIF":4.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photofermentative production of poly-β-hydroxybutyrate (PHB) by purple non-sulfur bacteria using olive oil by-products.","authors":"Gianmarco Mugnai, Luca Bernabò, Giulia Daly, Elisa Corneli, Alessandra Adessi","doi":"10.1186/s40643-025-00856-x","DOIUrl":"10.1186/s40643-025-00856-x","url":null,"abstract":"<p><p>This study evaluated the ability of six purple non-sulfur bacteria (PNSB) to convert olive oil by-products into poly-β-hydroxybutyrate (PHB). Strains were first independently cultivated in synthetic media with different carbon sources (acetic, lactic and malic acid) to assess their physiology and PHB production. Subsequently, their growth and PHB production using ingested pâté olive cake (IPOC) as a substrate were investigated. Transmission electron microscopy (TEM) observations were conducted on strains cultivated on IPOC to investigate their cell morphologies and inclusion bodies presence and size. Rhodopseudomonas palustris strains accumulated up to 6.8% w PHB/w cells with acetate and 0.86% w PHB/w cells with a daily productivity of 0.54 mg PHB L⁻¹ culture d⁻¹ on IPOC. In contrast, Cereibacter johrii and Cereibacter sphaeroides reached 58.64% w PHB/w cells and 65.45% w PHB/w cells with acetate, respectively, while C. sphaeroides achieved 21.48% w PHB/w cells and a daily productivity of 10.85 mg PHB L⁻¹ culture d⁻¹ when cultivated on IPOC. All strains exhibited growth and PHB accumulation in both synthetic media and IPOC substrate. Specifically, R. palustris strains 42OL, AV33 and CGA009 displayed growth capability in all substrates, while C. johrii strains 9Cis and PISA 7, and C. sphaeroides F17 showed promising PHB synthesis capabilities. TEM observations revealed that R. palustris strains, with smaller cell and inclusion body sizes, exhibited lower PHB accumulations, while C. johrii and C. sphaeroides strains, characterized by larger cells and inclusion bodies, demonstrated higher PHB production, recognizing them as promising candidates for PHB production using olive oil by-products. Further investigations under laboratory-scale conditions will be necessary to optimize operating parameters and develop integrated strategies for simultaneous PHB synthesis and the co-production of value-added products, thereby enhancing the economic feasibility of the process within a biorefinery framework.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"25"},"PeriodicalIF":4.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of extraction process and solvent polarities to enhance the recovery of phytochemical compounds, nutritional content, and biofunctional properties of Mentha longifolia L. extracts.","authors":"Meryem Tourabi, Khaoula Faiz, Rachid Ezzouggari, Bouchra Louasté, Mohammed Merzouki, Musaab Dauelbait, Mohammed Bourhia, Khalid S Almaary, Farhan Siddique, Badiaa Lyoussi, Elhoussine Derwich","doi":"10.1186/s40643-025-00859-8","DOIUrl":"10.1186/s40643-025-00859-8","url":null,"abstract":"<p><p>The current study attempted to evaluate the influence of three extraction processes such as Soxhlet, ultrasound-assisted extraction (UAE,) and cold maceration on one hand and solvent polarities (ethanol 70%, ethyl acetate, and water) on the other hand, vs. phytoconstituents and biofunctional properties of Mentha longifolia L. Noteworthy, all extracts were examined in terms of their chemical components, phenolic and flavonoid content, antioxidant and antimicrobial potentials. Notably, high-performance liquid chromatography coupled with array detector analysis (HPLC-DAD) showed the existence of many phenolic compounds. Hydro-ethanol extract (ETOH 70% (v/v)) prepared with maceration and Soxhlet process showed the ultimate rate of phenolic compounds coupled with the most powerful antioxidant and antimicrobial capacity. Notably, ETOH extract obtained with UAE showed important nutritional properties, particularly soluble carbohydrate (50.1 ± 0.70 mg/g dry weight), and soluble protein (26.5 ± 0.5 mg/g dry weight), while aqueous extract obtained by use of UAE extraction maximized pigment content. The outcome of this work showed that Soxhlet and maceration extraction processes with many polar solvents are more appropriate for M. longifolia extraction. M. longifolia possesses promising phytochemicals, which can be used in different sectors like food, pharmaceutical, and cosmetic formulations.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"24"},"PeriodicalIF":4.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) capped silver and zinc oxide nanoparticles and its molecular characterization.","authors":"Zabin K Bagewadi, Gouri H Illanad, T M Yunus Khan, Shaik Mohamed Shamsudeen, Sikandar I Mulla","doi":"10.1186/s40643-025-00857-w","DOIUrl":"10.1186/s40643-025-00857-w","url":null,"abstract":"<p><p>The current investigation reports anti-cancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) and L-Glutaminase capped nanoparticles. The highest L-Glutaminase production of 9.57 U/mL was achieved on the 4th day of fermentation when L-Glutamine was used as the sole carbon and nitrogen source. Enhanced recycling stability was observed after 6 cycles using L-Glutaminase immobilized in 3% agar and agarose matrices. Free and immobilized L- Glutaminase showed K_m of 13.89 ± 0.8 and 7.13 ± 0.3 mM and V_max of 18.40 ± 1.5 and 24.21 ± 1.7 U/mg respectively. L- Glutaminase capped silver (AgNP) and zinc oxide (ZnONP) nanoparticles were synthesized and structurally characterized using UV visible spectroscopy, FTIR, SEM-EDS, XRD and AFM. L- Glutaminase capped AgNP and ZnONP exhibited good thermal stability with five and three stages weight loss pattern respectively based on TGA. L-Glutaminase capped AgNP exhibited highest inhibitory activity against B. subtilis (45 <math><mo>±</mo></math> 0.5 mm) and E. coli (33 <math><mo>±</mo></math> 0.8 mm) whereas, L-Glutaminase capped ZnONP demonstrated highest inhibition against E. coli (30 <math><mo>±</mo></math> 0.3 mm) and B. cereus (25 <math><mo>±</mo></math> 0.5 mm). Increased nanoparticles concentration exhibited increased inhibitory potential as compared to wild L-Glutaminase and lowest MIC of 0.09 µg/mL was exhibited against B. cereus. L-Glutaminase capped nanoparticles demonstrated significant antioxidant properties through in-vitro ABTS and DPPH radical scavenging assays in a dosage-dependent manner. L-Glutaminase and capped AgNP and ZnONP, demonstrated pronounced cell cytotoxicity against MCF-7 cancerous cell line with 57.17 µg/mL, 8.13 µg/mL and 28.31 µg/mL IC₅₀ values respectively, suggesting promising properties as anticancer agents in enzyme-based therapy. The results reveal promising biological activities with potential applications in healthcare sector.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"23"},"PeriodicalIF":4.3,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing sustainable agriculture: the potential of seaweed-derived bio pesticides from marine biomass.","authors":"Sasikala Sekar, Sivakamavalli Jeyachandran, Jayant Giri, Mohammed Aman","doi":"10.1186/s40643-025-00849-w","DOIUrl":"10.1186/s40643-025-00849-w","url":null,"abstract":"<p><p>The exploitation of marine biomass, particularly seaweeds, presents significant opportunities for sustainable biopesticide production. Seaweeds, or macroalgae are diverse photosynthetic organisms found in marine environments, rich in bioactive compounds such as terpenoids, polyphenols, and sulphated polysaccharides. These compounds exhibit potent pesticide properties, offering environmentally friendly alternatives to conventional chemical pesticides. The biopesticides derived from seaweeds are biodegradable, exhibit low toxicity to non-target organisms, and align with the principles of environmental sustainability. The growing demand for organic and sustainable agricultural products further underscores the economic potential of seaweed-based biopesticides. However, challenges such as scalability of production, extraction efficiency, and standardization of bioactivity must be addressed to realize this potential fully. Advances in biorefinery approaches, extraction technologies, and formulation techniques are critical to overcoming these challenges. Additionally, ongoing research into the synergistic effects of different seaweed compounds and the development of innovative delivery systems will enhance the efficacy and commercial viability of seaweed-derived biopesticides. This review highlights the promising role of seaweeds in biopesticide production, emphasizing the need for continued research and interdisciplinary collaboration to integrate these natural compounds into sustainable agricultural practices.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"22"},"PeriodicalIF":4.3,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.","authors":"Shreya S Shettar, Zabin K Bagewadi, Mohammed Alasmary, Basheerahmed Abdulaziz Mannasaheb, Ibrahim Ahmed Shaikh, Aejaz Abdullatif Khan","doi":"10.1186/s40643-025-00860-1","DOIUrl":"10.1186/s40643-025-00860-1","url":null,"abstract":"<p><p>Enzyme deployment is proliferating extensively in industries owing to their environmentally friendly and easily degradable attributes. This article undertakes an exhaustive examination of wild subtilisin enzyme, covering purification, biochemical delineation, analytical techniques, and practical implementations. The purification methodology involved partial refinement, anionic exchange, and gel filtration chromatography, culminating in a purification factor of 3.406, corroborated by SDS-PAGE showcasing a molecular weight of ~ 42 kDa. Biochemical scrutiny unveiled the enzyme's response, with an optimal pH at 9 and temperature peak at 60 ℃. Various surfactants, metal ions, organic solvents and inhibitors exhibited notable efficacy. Substrate specificity and kinetics showcased the utmost specificity with N-Suc-F-A-A-F-pNA, registering K_m and V_max values of 0.731 ± 0.5 mM and 0.87 ± 9 × 10³ U/mg, respectively. Different bioanalytical techniquesproffered insights into structural and biophysical facets. Practical applications encompassed goat skin depilation, feather disintegration, blood clot dissolution, exemplifying the enzyme's multifaceted utility. To embark upon the elucidation of structure-function relationships, a three-dimensional model was devised through homology modelling, leveraging existing subtilisin structures (PDB: 3WHI). Molecular docking score of - 8.8 kcal/mol and dynamic simulations augmented the comprehension of molecular interactions with N-Suc-F-A-A-F-pNA. This research significantly contributes to unravelling the biochemical intricacies of wild subtilisin and underscores potential industrial and biomedical prowess. Subtilisin can be explored for its thrombolytic potential in several cardiovascular diseases. It may aid in the management of thrombosis by dissolving blood clots in conditions like deep pulmonary embolism, myocardial infarction, ischemic strokes, and in atherosclerosis by breaking down fibrin in arterial plaques, thus preventing heart attacks and strokes.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"21"},"PeriodicalIF":4.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}