Bioresources and Bioprocessing最新文献

{"title":"Correction: Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.","authors":"Shreya S Shettar, Zabin K Bagewadi, Mohammed Alasmary, Basheerahmed Abdulaziz Mannasaheb, Ibrahim Ahmed Shaikh, Aejaz Abdullatif Khan","doi":"10.1186/s40643-026-01048-x","DOIUrl":"10.1186/s40643-026-01048-x","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinational effect of gamma irradiation and sustainable bioactive absorbent bacterial cellulose pads impregnated with tangerine essential oil against some bacterial fish fillet-borne pathogens.","authors":"Reham M M Abdelkader, Doaa A Hamed, Hanan H Abdel-Khalek","doi":"10.1186/s40643-026-01061-0","DOIUrl":"10.1186/s40643-026-01061-0","url":null,"abstract":"<p><p>Antimicrobial food pads play a key role in the food packaging industry by improving food safety and quality. To control the growth of bacterial pathogens in fish fillets, this study aims to develop a bioactive system using absorbent food pads composed of bacterial cellulose (BC) infused with tangerine essential oil (TEO) as an antibacterial agent. The effects of active BC-pads, gamma irradiation, and their combination on artificially inoculated bacteria (previously isolated) were studied for 6 days at 4 °C using fish fillet samples as a model food system. The results revealed that the initial population counts of Klebsiella oxytoca, Serratia ficaria, Enterobacter cloacae, and Kocuria rosea, were 5.47, 5.63, 5.14, and 5.25 log CFU/g, respectively. These counts increased during the storage period, reaching 8.80 log CFU/g as a mean for all tested strains. After six days of storage, BC, BC/TEO, BC + 1.0 kGy, and BC/TEO + 1.0 kGy pads reduced the initial load of the four bacteria in the fish fillet samples by [1.59, 1.68, 1.92, 2.35], [3.21, 3.12, 3.36, 3.72], [3.53, 3.26, 3.26, 3.37] and [4.97, 5.02, 4.95, 4.83] log CFU/g, respectively. The combination of BC and TEO with low-dose gamma radiation (1.0 kGy) enhances the decontamination effectiveness of fish fillets through synergistic effects. FTIR analysis revealed a slight shift in the absorbance peaks of some functional group interactions and the formation of new bonds between BC and TEO in the presence of gamma irradiation. The study has successfully developed sustainable, eco-friendly, functional bioactive food pads that reduce microbial growth and prevent spoilage of fish fillets during storage.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple, rapid, resin-free platform for purification of vascular endothelial growth factor using a calcium-responsive fusion protein.","authors":"Jonghwan Lee, Heesun Park, KyuHyuk Im, Sanguine Byun, Sunghyun Kim","doi":"10.1186/s40643-026-01063-y","DOIUrl":"10.1186/s40643-026-01063-y","url":null,"abstract":"<p><strong>Background: </strong>Vascular endothelial growth factor (VEGF) is widely used in regenerative medicine and therapeutic research. However, the purification of recombinant VEGF largely relies on affinity chromatography, which requires expensive chromatographic columns, specialized equipment, and multistep processing. These column-based workflows increase operational complexity and cost, particularly for large-scale production. Therefore, the development of an alternative purification strategy to conventional chromatography-based purification for VEGF is needed.</p><p><strong>Findings: </strong>In this study, we developed a chromatography-free VEGF purification strategy using an anti-VEGF-scFv-calsequestrin (CSQ) fusion protein that enables calcium-dependent affinity precipitation. The fusion protein retained strong binding affinity for VEGF (K_d = 1.1 nM) while exhibiting rapid and reversible Ca²⁺-dependent polymerization. Upon CaCl₂ addition, the anti-VEGF-scFv-CSQ-VEGF complex rapidly formed aggregates, enabling efficient separation of VEGF from impurities. Using this strategy, VEGF was purified within 30 min with a purity of 94% and a yield of 93%. SEC-HPLC analysis confirmed a purity of 94.3%, and host cell protein contamination was reduced from 1.44 × 10⁴ ppm to 774 ppm. The fusion protein also maintained stable purification performance over five repeated cycles, with VEGF recovery consistently maintained above 85%.</p><p><strong>Conclusions: </strong>These findings demonstrate that the scFv-CSQ fusion protein enables rapid separation of VEGF through calcium-dependent polymerization. This column-free mechanism reduces operational cost and technical complexity, highlighting its potential as an alternative to conventional chromatography-based purification.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To treat or not to treat? Impact of hygienization on the microbiological safety of frass from black soldier fly larvae and yellow mealworm production aimed for fertilizer use.","authors":"Ann De Volder, Lotte Frooninckx, David Deruytter, Johan Ceusters, Dries Vandeweyer","doi":"10.1186/s40643-026-01030-7","DOIUrl":"10.1186/s40643-026-01030-7","url":null,"abstract":"<p><p>Sustainable expansion of the insect farming industry requires valorization of the generated frass, a by-product with fertilizer and soil improver potential. Commercialization is currently restricted by Regulation (EU) No 2021/1925, considering insect frass as animal manure and imposing a pathogen-reducing heat treatment. This study investigated the microbiological safety of frass from industrial black soldier fly larvae (BSFL) and yellow mealworm (YM) production, untreated and hygienized by drying, pelleting or composting. Results showed that despite high microbial counts for both viable cells and aerobic endospores, Salmonella spp. were absent in untreated frass, Escherichia coli counts were low compared to other manure types, while the number of Enterococci was similar. The hygienisation procedures induced little change in the total number of viable cells and aerobic endospores and exerted more impact on temperature-sensitive Enterobacteriaceae compared to more tolerant Enterococcaceae. Drying, pelleting and composting were not equally suited for hygienization of frass from both insect species due to differences in initial microbial load and physical composition. Generally, it could be concluded that YM frass will meet the EU microbiological criteria more easily than BSFL frass and may even do so without hygienization. In case hygienization is necessary, reduction of the number of E. coli by the applied treatment will be the factor determining regulation compliance. Compared to Enterococcaceae, E. coli abundance in frass is lower and the species is more efficiently reduced. Oven drying is suited for both BSFL and YM frass but is expensive as it requires an external heat source. Pelleting seems applicable to YM frass, BSFL frass requires preliminary heating to reduce its moisture content. Composting of BSFL frass may produce a safe product if temperatures reached throughout the process are high enough. Composted YM frass can be considered microbiologically more unstable/unsafe than untreated YM frass, due to water addition at the process start. It may be concluded that flexibility in treatment procedures for insect frass, aimed for use as fertilizer, can be allowed. Microbiological safety is ensured after reference treatment (70 °C–60 min) but can also be achieved after milder processing or even without treatment, which would reduce costs and preserve frass quality. </p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A robust hybrid data driven approach to model biochar yield in terms of biomass pyrolysis.","authors":"Kusum Yadav, Lulwah M Alkwai, Shahad Almansour, Mehrdad Mottaghi","doi":"10.1186/s40643-026-01058-9","DOIUrl":"10.1186/s40643-026-01058-9","url":null,"abstract":"<p><p>Biochar yield prediction plays a critical role in optimizing pyrolysis processes and advancing sustainable biomass utilization. This study introduces a hybrid machine learning framework that integrates Decision Tree models with four optimization strategies including Tabu Search, Ant Colony Optimization, Evolutionary Strategies, and Batch Bayesian Optimization (DT-BBO). A curated dataset of 211 samples was preprocessed using Leverage outlier detection and normalization to ensure model robustness. Among all tested models, the DT-BBO approach achieved the highest accuracy, with an R² of 0.98, an MSE of 1.9, and an AARE% of 2.2%, outperforming the other optimization techniques. SHAP analysis revealed that pyrolysis temperature, residence time, and ash content were the most influential parameters governing biochar yield. Comparative benchmarking against previously published models confirmed the superior predictive capability and stability of the proposed framework. The results demonstrate that the DT-BBO model offers a scalable, interpretable, and high-performing solution for biochar yield prediction, contributing to methodological innovation and sustainable biomass valorization.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification-free antifungal biocontrol platform using engineered Saccharomyces cerevisiae secreting Trichoderma atroviride chitinase.","authors":"Ha-Yeon Song, Dae-Hyuk Kim, Jung-Mi Kim, Ji Young Kang","doi":"10.1186/s40643-026-01054-z","DOIUrl":"10.1186/s40643-026-01054-z","url":null,"abstract":"<p><p>Chitinases from Trichoderma species exhibit strong antifungal activity and high biocontrol potential, yet their industrial utilization has been constrained by low heterologous secretion efficiency and costly purification processes. In this study, the Generally Recognized As Safe yeast Saccharomyces cerevisiae Y2805 was engineered to secrete chitinase Tch36 from a Korean isolate of T. atroviride, using a rice α-amylase signal peptide under a constitutive glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter. When cultivated in glycerol–colloidal chitin medium, the recombinant yeast exhibited approximately a fivefold increase in measurable chitinase activity relative to the SC mock control, whereas the empty-vector control showed only a modest increase. The crude, non-concentrated culture filtrate (approximately 1000 U L⁻1) displayed statistically significant antifungal activity against 12 fungal species, including 9 phytopathogens and 3 opportunistic Aspergillus species. Among the phytopathogens, Fusarium graminearum was one of the most strongly inhibited species, showing approximately 40–50% suppression of colonial growth on solid medium. In liquid culture, microscopy-based germination assays revealed ≥ 63% inhibition of early hyphal elongation in Botrytis cinerea and A. niger. All antifungal effects were evaluated relative to filtrates from the empty-vector control prepared under equivalent dilution conditions. The culture filtrate also enhanced protoplast formation, providing direct evidence of chitinase-associated cell wall weakening. Collectively, these results establish a purification-free, yeast-based heterologous secretion platform capable of producing active Trichoderma chitinase with inhibitory effects on diverse plant- and animal-associated fungi. This strategy has practical potential for biocontrol applications and for sustainable bioprocessing technologies based on microbial chitinase production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-root Eichhornia crassipes waste plants dual-purpose resource utilization: green preparation of magnetic carbon quantum dots for heavy metal deep removal.","authors":"Yihong Guo, Mingxin Cui, Hongjun Yang, Jun Chen, Sen Lin","doi":"10.1186/s40643-026-01064-x","DOIUrl":"10.1186/s40643-026-01064-x","url":null,"abstract":"<p><p>Long-root Eichhornia crassipes, an aquatic plant with extensive root systems, has demonstrated potential in remediating eutrophicated water bodies. However, improper disposal of large quantities of discarded plants post-remediation can easily cause secondary pollution. In this study, rapidly recoverable magnetic carbon quantum dots (MCQDs) were greenly synthesized via hydrothermal treatment of long-root Eichhornia crassipes and subsequent coupling with Fe3O4 nanoparticles. Adsorption experiments demonstrated that MCQDs displayed a commendable adsorption capacity for various heavy metals in municipal wastewater under the synergistic effect of CODs and Fe3O4. Mechanistic analyses revealed that the adsorption involved chemical binding with oxygen-containing functional groups and amino groups on the surface of MCOQs. The saturated MCQDs were efficiently recovered within 5 min using an applied magnetic field, offering a practical solution for nanoparticle recovery. This study provides valuable insights into the green resource utilization of Long-root Eichhornia crassipes based on CQD technology.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered NLS-PBase system boosts stability and productivity in recombinant cell lines.","authors":"Yanan Zhou, Yuan Tian, Qingyuan Ran, Qian Ye, Wen-Song Tan","doi":"10.1186/s40643-026-01056-x","DOIUrl":"10.1186/s40643-026-01056-x","url":null,"abstract":"<p><p>Chinese hamster ovary (CHO) cells constitute the industry-standard platform for the production of complex therapeutic proteins, yet genomic heterogeneity arising from random integration leads to clonal variability and unstable expression, necessitating a robust cell line development process to efficiently isolate stable, high-expressing clones. By employing an optimized integration strategy, recombinant cell line performance can be enhanced through improved genomic stability and increased productivity. In this study, GFP reporter analysis demonstrated that the PiggyBac system significantly boosts the yield of both stable and high-expression clones. Subsequently, transposase modified with a nuclear localization signal (NLS) exhibited superior stability in recombinant cell lines and polyclonal pools. The nucleoplasmin NLS resulted in transgene integration into genomic loci that promote enhanced and more stable expression, thereby improving clonal distribution. Furthermore, this strategy also increased recombinant mAb expression by 97% in pools while enhancing average and specific productivity in derived cell lines. Additionally, recombinant suspension cells generated with the optimized system exhibited comparable performance, with over 50% of minipools showing robust growth. Overall, these findings highlight the promising utility of the NLS-optimized PiggyBac system for improving transgene stability and expression while streamlining the cell line screening process.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-rapid and high-titer biomanufacturing of trehalose 6-phosphate by an in vitro synthetic biology platform.","authors":"Bohua Liu, Qingqing Guo, Shuo Wang, Ting Shi, Fuping Lu, Yi-Heng P Job Zhang","doi":"10.1186/s40643-026-01057-w","DOIUrl":"10.1186/s40643-026-01057-w","url":null,"abstract":"<p><p>Trehalose 6-phosphate (T6P), often referred to as plant insulin, serves as a central signaling molecule that regulates carbon partitioning and sucrose flux in plants, thereby influencing key agronomic traits, such as grain yield and drought resilience. Foliar spraying of T6P has been shown to significantly enhance yield and stress tolerance of numerous grains and vegetables. To circumvent the dependency on coenzymes in natural T6P synthesis, we designed and validated an in vitro new-to-nature, coenzyme-free, minimal enzymatic pathway for the biosynthesis of T6P from maltose and polyphosphate. This three-enzyme cocktail contained maltose phosphorylase, trehalose 6-phosphate phosphorylase, and polyphosphate glucokinase, and did not involve any costly coenzymes, such as ATP or UDP. Through systematic optimization of experimental parameters (including pH, temperature, Mg²⁺, phosphate concentration, and enzyme ratios), a 93% molar yield of T6P was achieved from 10 g/L maltose. The scale-up of this in vitro bioprocess to a 100-mL bioreactor with 200 g/L maltose enabled the production of up to 541 mM T6P (i.e., 252 g/L T6P disodium salt) within two hours, corresponding to a very high volumetric productivity of 126 g/L/h. This study established a scalable and cost-competitive in vitro biomanufacturing of T6P. Chemical intervention based on timed foliar spraying of T6P to cultivated crops offers a simpler and safer agricultural practice compared to genetic modification of crops.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo and in vitro antidiabetic effects of phlorizin and its green-synthesized phlorizin-selenium nanoparticles in male rats: mechanistic involvements and nanoparticles characterization.","authors":"Elshahat A Toson, Entsar A Saad, Mohammad M Mashaly, Hadeer A Omar","doi":"10.1186/s40643-026-01034-3","DOIUrl":"10.1186/s40643-026-01034-3","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}