Bioresources and Bioprocessing最新文献_第9页

Optimisation of sugar and solid biofuel co-production from almond tree prunings by acid pretreatment and enzymatic hydrolysis 通过酸预处理和酶水解优化杏树枝条的糖和固体生物燃料联合生产

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-03-11 DOI: 10.1186/s40643-024-00743-x

Manuel Cuevas-Aranda, Mª Lourdes Martínez-Cartas, Fahd Mnasser, Adnan Asad Karim, Sebastián Sánchez

{"title":"Optimisation of sugar and solid biofuel co-production from almond tree prunings by acid pretreatment and enzymatic hydrolysis","authors":"Manuel Cuevas-Aranda, Mª Lourdes Martínez-Cartas, Fahd Mnasser, Adnan Asad Karim, Sebastián Sánchez","doi":"10.1186/s40643-024-00743-x","DOIUrl":"https://doi.org/10.1186/s40643-024-00743-x","url":null,"abstract":"<p>Almond pruning biomass is an important agricultural residue that has been scarcely studied for the co-production of sugars and solid biofuels. In this work, the production of monosaccharides from almond prunings was optimised by a two-step process scheme: pretreatment with dilute sulphuric acid (0.025 M, at 185.9–214.1 ℃ for 0.8–9.2 min) followed by enzyme saccharification of the pretreated cellulose. The application of a response surface methodology enabled the mathematical modelling of the process, establishing pretreatment conditions to maximise both the amount of sugar in the acid prehydrolysate (23.4 kg/100 kg raw material, at 195.7 ℃ for 3.5 min) and the enzymatic digestibility of the pretreated cellulose (45.4%, at 210.0 ℃ for 8.0 min). The highest overall sugar yield (36.8 kg/100 kg raw material, equivalent to 64.3% of all sugars in the feedstock) was obtained with a pretreatment carried out at 197.0 ℃ for 4.0 min. Under these conditions, moreover, the final solids showed better properties for thermochemical utilisation (22.0 MJ/kg heating value, 0.87% ash content, and 72.1 mg/g moisture adsorption capacity) compared to those of the original prunings.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"27 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization 提高嗜热几丁质酶的内切活性以产生高聚合度的壳寡糖

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-03-07 DOI: 10.1186/s40643-024-00735-x

Feifei Guan, Xiaoqian Tian, Ruohan Zhang, Yan Zhang, Ningfeng Wu, Jilu Sun, Honglian Zhang, Tao Tu, Huiying Luo, Bin Yao, Jian Tian, Huoqing Huang

{"title":"Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization","authors":"Feifei Guan, Xiaoqian Tian, Ruohan Zhang, Yan Zhang, Ningfeng Wu, Jilu Sun, Honglian Zhang, Tao Tu, Huiying Luo, Bin Yao, Jian Tian, Huoqing Huang","doi":"10.1186/s40643-024-00735-x","DOIUrl":"https://doi.org/10.1186/s40643-024-00735-x","url":null,"abstract":"<p>Thermophilic endo-chitinases are essential for production of highly polymerized chitooligosaccharides, which are advantageous for plant immunity, animal nutrition and health. However, thermophilic endo-chitinases are scarce and the transformation from exo- to endo-activity of chitinases is still a challenging problem. In this study, to enhance the endo-activity of the thermophilic chitinase Chi304, we proposed two approaches for rational design based on comprehensive structural and evolutionary analyses. Four effective single-point mutants were identified among 28 designed mutations. The ratio of (GlcNAc)<sub>3</sub> to (GlcNAc)<sub>2</sub> quantity (DP<sub>3/2</sub>) in the hydrolysates of the four single-point mutants undertaking colloidal chitin degradation were 1.89, 1.65, 1.24, and 1.38 times that of Chi304, respectively. When combining to double-point mutants, the DP<sub>3/2</sub> proportions produced by F79A/W140R, F79A/M264L, F79A/W272R, and M264L/W272R were 2.06, 1.67, 1.82, and 1.86 times that of Chi304 and all four double-point mutants exhibited enhanced endo-activity. When applied to produce chitooligosaccharides (DP ≥ 3), F79A/W140R accumulated the most (GlcNAc)<sub>4</sub>, while M264L/W272R was the best to produce (GlcNAc)<sub>3</sub>, which was 2.28 times that of Chi304. The two mutants had exposed shallower substrate-binding pockets and stronger binding abilities to shape the substrate. Overall, this research offers a practical approach to altering the cutting pattern of a chitinase to generate functional chitooligosaccharides.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"66 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140054765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2 优化内生真菌 Aspergillus sp. SPH2 产生的杀菌和杀螨代谢物

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-03-05 DOI: 10.1186/s40643-024-00745-9

Nicolas Reyes Castillo, Carmen E. Díaz, M. Fe Andres, Juan Imperial, Félix Valcárcel, Ana Azucena González Coloma

{"title":"Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2","authors":"Nicolas Reyes Castillo, Carmen E. Díaz, M. Fe Andres, Juan Imperial, Félix Valcárcel, Ana Azucena González Coloma","doi":"10.1186/s40643-024-00745-9","DOIUrl":"https://doi.org/10.1186/s40643-024-00745-9","url":null,"abstract":"<p>The endophytic fungus <i>Aspergillus</i> sp. SPH2 was isolated from the stems of the endemic plant <i>Bethencourtia palmensis</i> and its extracts were found to have strong fungicidal effects against <i>Botrytis cinerea</i> and ixodicidal effects against <i>Hyalomma lusitanicum</i> at different fermentation times. In this study, the fungus was grown using three different culture media and two methodologies, Microparticulate Enhancement Cultivation (MPEC) and Semi-Solid-State Fermentation (Semi-SSF), to increase the production of secondary metabolites during submerged fermentation. The addition of an inert support to the culture medium (Semi-SSF) resulted in a significant increase in the extract production. However, when talcum powder was added to different culture media, unexpected results were observed, with a decrease in the production of the biocompounds of interest. Metabolomic analyses showed that the production of aspergillic, neoaspergillic, and neohydroxyaspergillic acids peaked in the first few days of fermentation, with notable differences observed among the methodologies and culture media. Mellein production was particularly affected by the addition of an inert support to the culture medium. These results highlight the importance of surface properties and morphology of spores and mycelia during fermentation by this fungal species.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"33 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140045255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential use of Ulva intestinalis-derived biochar adsorbing phosphate ions in the cultivation of winter wheat Tristicum aestivum 吸附磷酸盐离子的肠莼生物炭在冬小麦种植中的潜在用途

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-03-04 DOI: 10.1186/s40643-024-00741-z

Natalia Niedzbała, Ewa Lorenc-Grabowska, Piotr Rutkowski, Jacek Chęcmanowski, Anna Szymczycha-Madeja, Maja Wełna, Izabela Michalak

{"title":"Potential use of Ulva intestinalis-derived biochar adsorbing phosphate ions in the cultivation of winter wheat Tristicum aestivum","authors":"Natalia Niedzbała, Ewa Lorenc-Grabowska, Piotr Rutkowski, Jacek Chęcmanowski, Anna Szymczycha-Madeja, Maja Wełna, Izabela Michalak","doi":"10.1186/s40643-024-00741-z","DOIUrl":"https://doi.org/10.1186/s40643-024-00741-z","url":null,"abstract":"<p>In this work, the properties of biochar produced from green macroalga <i>Ulva intestinalis</i> by pyrolysis were studied at temperatures of 300, 500, and 700 °C. This biochar was characterized in terms of multielemental composition, BET surface area, total pore volume, and biosorption properties toward phosphate ions. Biochar produced at 700 °C–25 m<sup>2</sup>/g had the highest surface area. The kinetics and isotherms of sorption processes of phosphate ions as sorbate by these sorbents were investigated. Modified biochar was able to remove 84.3% of phosphate ions from wastewater, whereas non-modified biochar—only 40.6%. Hence, biochar enriched with phosphate ions can serve as a valuable soil amendment. Pot experiments performed on winter wheat (<i>Triticum aestivum</i>) with a 3% addition of dry <i>Ulva intestinalis</i>, pristine biochar, and Mg-modified biochar enriched with phosphate ions showed that these amendments stimulated plant growth (length and fresh weight of plants) as well as enlarging the chlorophyll content in leaves. Our results indicate that the production of biochar (pristine and Mg-impregnated) is a sustainable option to valorize the biomass of seaweeds, and to recycle phosphorus from wastewater.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"24 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140034395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction 合理引入非经典氨基酸，提高 LmrR 在亨利反应中的催化活性

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-29 DOI: 10.1186/s40643-024-00744-w

Lan Wang, Mengting Zhang, Haidong Teng, Zhe Wang, Shulin Wang, Pengcheng Li, Jianping Wu, Lirong Yang, Gang Xu

{"title":"Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction","authors":"Lan Wang, Mengting Zhang, Haidong Teng, Zhe Wang, Shulin Wang, Pengcheng Li, Jianping Wu, Lirong Yang, Gang Xu","doi":"10.1186/s40643-024-00744-w","DOIUrl":"https://doi.org/10.1186/s40643-024-00744-w","url":null,"abstract":"<p>The use of enzymes to catalyze Henry reaction has advantages of mild reaction conditions and low contamination, but low enzyme activity of promiscuous catalysis limits its application. Here, rational design was first performed to identify the key amino acid residues in Henry reaction catalyzed by Lactococcal multidrug resistance Regulator (LmrR). Further, non-canonical amino acids were introduced into LmrR, successfully obtaining variants that enhanced the catalytic activity of LmrR. The best variant, V15CNF, showed a 184% increase in enzyme activity compared to the wild type, and was 1.92 times more effective than the optimal natural amino acid variant, V15F. Additionally, this variant had a broad substrate spectrum, capable of catalyzing reactions between various aromatic aldehydes and nitromethane, with product yielded ranging from 55 to 99%. This study improved enzymatic catalytic activity by enhancing affinity between the enzyme and substrates, while breaking limited types of natural amino acid residues by introducing non-canonical amino acids into the enzyme, providing strategies for molecular modifications.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"28 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140018771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling the behavior of monoclonal antibodies on hydrophobic interaction chromatography resins 单克隆抗体在疏水相互作用色谱树脂上的行为建模

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-15 DOI: 10.1186/s40643-024-00738-8

Douglas Nolan, Thomas R. Chin, Mick Eamsureya, Sheldon Oppenheim, Olga Paley, Christina Alves, George Parks

{"title":"Modeling the behavior of monoclonal antibodies on hydrophobic interaction chromatography resins","authors":"Douglas Nolan, Thomas R. Chin, Mick Eamsureya, Sheldon Oppenheim, Olga Paley, Christina Alves, George Parks","doi":"10.1186/s40643-024-00738-8","DOIUrl":"https://doi.org/10.1186/s40643-024-00738-8","url":null,"abstract":"<p>Monoclonal antibodies (mAbs) require a high level of purity for regulatory approval and safe administration. High-molecular weight (HMW) species are a common impurity associated with mAb therapies. Hydrophobic interaction chromatography (HIC) resins are often used to remove these HMW impurities. Determination of a suitable HIC resin can be a time and resource-intensive process. In this study, we modeled the chromatographic behavior of seven mAbs across 13 HIC resins using measurements of surface hydrophobicity, surface charge, and thermal stability for mAbs, and hydrophobicity and zeta-potential for HIC resins with high fit quality (adjusted <i>R</i><sup>2</sup> > 0.80). We identified zeta-potential as a novel key modeling parameter. When using these models to select a HIC resin for HMW clearance of a test mAb, we were able to achieve 60% HMW clearance and 89% recovery. These models can be used to expedite the downstream process development for mAbs in an industry setting.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"87 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139750486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial monitoring of hydrolysis in a plug-flow bioreactor: a support for flexible operation? 塞流式生物反应器水解过程的空间监测：为灵活操作提供支持？

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-14 DOI: 10.1186/s40643-024-00740-0

Theresa Menzel, Peter Neubauer, Stefan Junne

引用次数: 0

Optimization of Clostridium beijerinckii semi-solid fermentation of rape straw to produce butyric acid by genome analysis 通过基因组分析优化贝氏梭菌半固态发酵油菜秸秆生产丁酸的工艺

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-14 DOI: 10.1186/s40643-024-00742-y

Hui Kou, Jia Zheng, Guangbin Ye, Zongwei Qiao, Kaizheng Zhang, Huibo Luo, Wei Zou

{"title":"Optimization of Clostridium beijerinckii semi-solid fermentation of rape straw to produce butyric acid by genome analysis","authors":"Hui Kou, Jia Zheng, Guangbin Ye, Zongwei Qiao, Kaizheng Zhang, Huibo Luo, Wei Zou","doi":"10.1186/s40643-024-00742-y","DOIUrl":"https://doi.org/10.1186/s40643-024-00742-y","url":null,"abstract":"<p>Butyric acid is a volatile saturated monocarboxylic acid, which is widely used in the chemical, food, pharmaceutical, energy, and animal feed industries. This study focuses on producing butyric acid from pre-treated rape straw using simultaneous enzymatic hydrolysis semi-solid fermentation (SEHSF). <i>Clostridium beijerinckii</i> BRM001 screened from pit mud of Chinese nongxiangxing baijiu was used. The genome of <i>C. beijerinckii</i> BRM001 was sequenced and annotated. Using rape straw as the sole carbon source, fermentation optimization was carried out based on the genomic analysis of BRM001. The optimized butyric acid yield was as high as 13.86 ± 0.77 g/L, which was 2.1 times higher than that of the initial screening. Furthermore, under optimal conditions, non-sterile SEHSF was carried out, and the yield of butyric acid was 13.42 ± 0.83 g/L in a 2.5-L fermentor. This study provides a new approach for butyric acid production which eliminates the need for detoxification of straw hydrolysate and makes full use of the value of fermentation waste residue without secondary pollution, making the whole process greener and more economical, which has a certain industrial potential.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"254 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139750605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat flour-derived amyloid fibrils for efficient removal of organic dyes from contaminated water 提取自小麦粉的淀粉样纤维可高效去除受污染水体中的有机染料

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-14 DOI: 10.1186/s40643-024-00737-9

Dan-Dan Yang, Fu-Xiang Chang, Bo-Fan Zhang, Yang-Chun Yong

{"title":"Wheat flour-derived amyloid fibrils for efficient removal of organic dyes from contaminated water","authors":"Dan-Dan Yang, Fu-Xiang Chang, Bo-Fan Zhang, Yang-Chun Yong","doi":"10.1186/s40643-024-00737-9","DOIUrl":"https://doi.org/10.1186/s40643-024-00737-9","url":null,"abstract":"<p>Amyloid fibrils derived from different proteins have been proved as a promising material for adsorption of various pollutants from wastewater, which showed advantages of low cost and eco-friendliness. However, most of the amyloid fibrils derived from animal-based proteins with high environmental footprint, while more sustainable amyloid fibrils derived from plant materials are desirable. In this study, a plant-derived amyloid fibril was extracted from the commonly used wheat flour with a simple and scalable protein purification and fibrillization process. Interestingly, the amyloid fibrils showed good adsorption capacity towards typical organic dyes (Eosin Y (EY) and Congo red (CR)) from contaminated water. Adsorption kinetic analysis indicated the adsorption process to EY or CR by wheat flour amyloid well fitted with a pseudo-second-order model. The adsorption also followed a Langmuir isothermal model with adsorption capacities of 333 mg/g and 138 mg/g towards CR and EY, respectively. This work demonstrated the feasibility to utilize the plant-based amyloid fibril for organic dyes removal from contaminated water, which provided an affordable, sustainable and scalable tool for organic dyes removal from wastewater.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"32 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139750492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen type II solution extracted from supercritical carbon dioxide decellularized porcine cartilage: regenerative efficacy on post-traumatic osteoarthritis model 从超临界二氧化碳脱细胞猪软骨中提取的 II 型胶原蛋白溶液：对创伤后骨关节炎模型的再生功效

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-02-03 DOI: 10.1186/s40643-024-00731-1

Srinivasan Periasamy, Yun-Ju Chen, Dur-Zong Hsu, Dar-Jen Hsieh

{"title":"Collagen type II solution extracted from supercritical carbon dioxide decellularized porcine cartilage: regenerative efficacy on post-traumatic osteoarthritis model","authors":"Srinivasan Periasamy, Yun-Ju Chen, Dur-Zong Hsu, Dar-Jen Hsieh","doi":"10.1186/s40643-024-00731-1","DOIUrl":"https://doi.org/10.1186/s40643-024-00731-1","url":null,"abstract":"<p>Osteoarthritis (OA) of the knee is a common degenerative articular disorder and is one of the main causes of pain and functional disability. Cartilage damage is frequently linked to elevated osteoarthritis incidence. Supercritical carbon dioxide (scCO<sub>2</sub>) decellularized cartilage graft produced from the porcine cartilage is an ideal candidate for cartilage tissue engineering. In the present study, we derived collagen type II (Col II) solution from the scCO<sub>2</sub> decellularized porcine cartilage graft (dPCG) and compared its efficacy with hyaluronic acid (HA) in the surgical medial meniscectomy (MNX) induced post-traumatic osteoarthritis (PTOA) model. Dose-dependent attenuation of the OA (12.3 ± 0.8) progression was observed in the intra‐articular administration of Col II solution (7.3 ± 1.2) which significantly decreased the MNX-induced OA symptoms similar to HA. The pain of the OA group (37.4 ± 2.7) was attenuated dose-dependently by Col II solution (45.9 ± 4.1) similar to HA (43.1 ± 3.5) as evaluated by a capacitance meter. Micro‐CT depicted a dose-dependent attenuation of articular cartilage damage by the Col II solution similar to HA treatment. A significant (<i>p</i> < 0.001) dose-dependent elevation in the bone volume was also observed in Col II solution-treated OA animals. The protective competence of Col II solution on articular cartilage damage is due to its significant (<i>p</i> < 0.001) increase in the expression of type II collagen, aggrecan and SOX‐9 similar to HA. To conclude, intra‐articular administration of type II collagen solution and HA reestablished the injured cartilage and decreased osteoarthritis progression in the experimental PTOA model.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"174 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0