Bioresources and Bioprocessing最新文献

{"title":"Integrated evaluation of antifungal activity of pomegranate peel polyphenols against a diverse range of postharvest fruit pathogens.","authors":"Muhammad Nawaz, Junkun Pan, Hui Liu, Muhammad Jawad Umer, Jiechao Liu, Wenbo Yang, Zhenzhen Lv, Qiang Zhang, Zhonggao Jiao","doi":"10.1186/s40643-025-00874-9","DOIUrl":"https://doi.org/10.1186/s40643-025-00874-9","url":null,"abstract":"<p><p>The search for safe, natural antimicrobial agents has received significant attention for controlling postharvest diseases in fruits. This study evaluated the antifungal activity of pomegranate peel extracts (PPE) against 9 pathogenic fungi by different methods. Additionally, the key antifungal polyphenol compounds were identified. Results revealed that the n-hexane partitioned fraction of ethanolic extract exhibited the highest inhibition efficacy. A total 36 polyphenols, including 10 newly discovered compounds in pomegranate peel were recognized. Among these, 9 specific polyphenols were found to be relatively abundant in the n-hexane fraction, highlighting their potential as primary antifungal agents. Notably, the newly identified polyphenol compound nobiletin demonstrated the strongest inhibitory effects against Colletotrichum gloeosporioides, Rhizopus stolonifer and Aspergillus niger with inhibition diameters of 12.2, 12.0, and 12.5 mm, respectively. Salidroside exhibited significant antifungal activity against Monilinia fructicola and A. niger (inhibition diameters > 12.0 mm). Furthermore, cinnamic acid that was enriched in hexane fraction, showed the highest inhibitory zones against Alternaria alternata, C. gloeosporioides and Botryosphaeria dothidea (11.8 ~ 12.7 mm). Caffeic acid and protocatechuic acid both displayed the lowest minimum inhibitory concentration (MIC) on Penicillium expansum. The study demonstrated the potential of PPE, particularly the n-hexane fraction, as a broad-spectrum natural antifungal agent for postharvest disease management.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"34"},"PeriodicalIF":4.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of novel MgO-ZnO nanocomposite using Pluchea indica leaf extract and study of their biological activities.","authors":"Samy Selim, Mohammed S Almuhayawi, Amna A Saddiq, Mohammed H Alruhaili, Ebrahim Saied, Mohamed H Sharaf, Muyassar K Tarabulsi, Soad K Al Jaouni","doi":"10.1186/s40643-025-00848-x","DOIUrl":"https://doi.org/10.1186/s40643-025-00848-x","url":null,"abstract":"<p><p>The biosynthesis of bimetallic nanoparticles using plant extracts has garnered significant attention due to their eco-friendly and cost-effective nature. This study aimed to biosynthesize magnesium oxide-zinc oxide nanocomposite (MgO-ZnO nanocomposite) using Pluchea indica leaf extract for the first time, with a focus on characterizing its physicochemical properties and evaluating its biological activities. The biosynthesized MgO-ZnO nanocomposite was fully characterized, revealing an absorbance peak at 300 nm using UV-vis spectroscopy. Transmission electron microscopy (TEM) confirmed particle stability within the size range of 5-35 nm. Cytotoxicity analysis on the Wi 38 normal cell line demonstrated an IC₅₀ value of 179.13 µg/mL, indicating good biosafety. The nanocomposite exhibited potent anticancer activity, with IC₅₀ values of 73.61 µg/mL and 31.25 µg/mL against Hep-G2 and MCF-7 cancer cell lines, respectively. Antibacterial assays revealed activity against Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Candida albicans, with minimum inhibitory concentrations (MICs) ranging from 31.25 to 250 µg/mL. Furthermore, the nanocomposite displayed antioxidant activity with an IC₅₀ value of 175 µg/mL, as determined by the DPPH assay. In conclusion, the successful synthesis of the MgO-ZnO nanocomposite using P. indica leaf extract demonstrates its potential as a safe and effective agent for concentration-dependent antioxidants, antibacterial, and anticancer applications. This study highlights the versatility of plant-mediated biosynthesis in developing functional nanomaterials for biomedical use.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"33"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statistical and neural network modeling of β-glucanase production by Streptomyces albogriseolus (PQ002238), and immobilization on chitosan-coated magnetic microparticles.","authors":"Nourhan H Elshami, Ghadir S El-Housseiny, Mahmoud A Yassien, Nadia A Hassouna","doi":"10.1186/s40643-025-00862-z","DOIUrl":"https://doi.org/10.1186/s40643-025-00862-z","url":null,"abstract":"<p><p>β-Glucanases are a series of glycoside hydrolases (GHs) that are of special interest for various medical and biotechnological applications. Numerous β-glucanases were produced by different types of microorganisms. Particularly, bacterial β-glucanases have the privilege of being stable, easily produced, and suitable for large-scale production. This study aimed for finding potent β-glucanase producing bacterial strains and optimizing its production. Soil samples from Egyptian governorates were screened for such strains, and 96 isolates were collected. The β-glucanase activity was qualitatively assessed and quantitatively measured using 3,5-dinitrosalicylic acid (DNS) method. The highest β-glucanase producing strain (0.74 U/ml) was identified as Streptomyces albogriseolus S13-1. The optimum incubation period and temperature, determined one-variable at a time, were estimated as 4 d and 45 ͦ C, respectively. Similarly, yeast β-glucan and beef extract were selected as the best carbon and nitrogen sources, with enzymatic activities of 0.74 and 1.12 U/ml, respectively. Other fermentation conditions were optimized through response surface methodology (RSM); D-optimal design (DOD) with a total of 28 runs. The maximum experimental β-glucanase activity (1.3 U/ml) was obtained with pH 6.5, inoculum volume of 0.5% v/v, agitation speed of 100 rpm, carbon concentration of 1% w/v, and nitrogen concentration of 0.11% w/v. This was 1.76-fold higher compared to unoptimized conditions. Using the same experimental matrix, an artificial neural network (ANN) was built to predict β-glucanase production by the isolated strain. Predicted β-glucanase levels by RSM and ANN were 1.79 and 1.32 U/ml, respectively. Both models slightly over-estimated production levels, but ANN showed higher predictivity and better performance metrics. The enzyme was partially purified through acetone precipitation, characterized, and immobilized on chitosan-coated iron oxide microparticles. The optimal pH and temperature for enzyme activity were 5 and 50 °C, respectively. The immobilized enzyme showed superior characters such as higher stability at temperatures 50, 60, and 70 °C compared to the free enzyme, and satisfactory reusability, losing only 30% of activity after 6 cycles of reuse.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"32"},"PeriodicalIF":4.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignocellulose-derived inhibitors can extend residence of Clostridium beijerinckii in active solventogenic state.","authors":"K Koppova, L Burianova, P Patakova, B Branska","doi":"10.1186/s40643-025-00871-y","DOIUrl":"https://doi.org/10.1186/s40643-025-00871-y","url":null,"abstract":"<p><p>Lignocellulose is a promising renewable resource for producing platform chemicals, such as acetone, butanol, and ethanol, via ABE fermentation by solventogenic clostridia. This study investigates the effects of common lignocellulose derived inhibitory compounds: ferulic acid, coumaric acid, and furfural on Clostridium beijerinckii. Dual-staining with propidium iodide and CFDA, combined with flow cytometry, was employed to assess physiological variability. The results showed that phenolic acid-induced stress helped maintain a higher proportion of viable cells during the production phase, enhancing solvent yields and reducing sporulation. At 0.4 g/L, ferulic and coumaric acids did not reduce cell viability; however, coumaric acid exposure led to an acid-crash profile. Conversely, a more robust inoculum exposed to both phenolic acids simultaneously exhibited effects similar to ferulic acid alone, including slower viability decline, reduced growth and sporulation, and improved solvent production. Furfural exposure at 1.5 g/L resulted in immediate viability loss in 20% of the population, though the overall decline accompanied by the highest sporulation rate occurred later than in the control. Additionally, furfural transformation was slower, suppressing butyrate production and reducing solvent production by 13%. This study suggests that delaying cell death mechanism may explain the stimulatory effects of inhibitors, advancing lignocellulose use in the future.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"31"},"PeriodicalIF":4.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of network pharmacology and experimental validation to explore the pharmacological mechanism of andrographolide against asthma.","authors":"Qian Yu, LiHong Zhu, XuChun Ding, YaFang Lou","doi":"10.1186/s40643-025-00869-6","DOIUrl":"10.1186/s40643-025-00869-6","url":null,"abstract":"<p><p>Andrographolide (AG), one of the main active components of Andrographis paniculata (Burm.f.) Wall. ex Nees, has been proved to possess the pharmacological function of anti-inflammation in multiple disease including asthma. But the potential mechanism is still not clear. In this study, network pharmacology, molecular docking and experimental validation were utilized to explore the molecular mechanism of AG in the treatment of asthma. AG-related targets and asthma-related targets were screened by Swiss Target Prediction, DrugBank, STITCH, OMIM, Genecards and TTD databases. A protein-protein interaction (PPI) network was obtained through the STRING Database. The plug-in of \"Network Analyzer\" in Cytoscape 3.7.1 software was used to conduct the topological analysis. GO enrichment and KEGG pathway analysis were achieved by Metascape database and Bioinformatics platform. The target-pathway network was acquired by Cytoscape 3.7.1 software. The binding affinity between AG and the target genes was evaluated by Molecular docking with AutoDockTools 1.5.6. Flow cytometry was also used to verify the mechanism behind the treatment of asthma by AG, which was predicted in network pharmacology. In total, 38 targets were identified as potential targets of AG against asthma. The top 10 targets revealed by PPI are: IL-6, IL-1B, NFKB1, MMP9, CDK2, CREBBP, MAP2K1, JAK1, AR, PRKCA. GO and KEGG analysis showed that AG treatment of asthma mainly involved protein phosphorylation, peptidyl-serine phosphorylation, peptidyl-amino acid modification and other biological processes. The main signaling pathways are Th17 cell differentiation, JAK-STAT signaling pathway and PI3K-Akt signaling pathway. Molecular docking showed that AG has higher affinity with MMP9, PRKCA, JAK2, LTGAL and LRRK2. Flow cytometry showed that Th17 cell differentiation may be the potential target of AG in the treatment of asthma. This study successfully revealed the underlying target genes and mechanism involved in the treatment of asthma for AG, providing a reference and guidance for future mechanism research.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"30"},"PeriodicalIF":4.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the in vitro activity of extracted Euphorbia trigona via Supercritical Fluid Extraction against pathogenic yeasts, obesity, cancer, and its wound healing properties.","authors":"Abdulrahman S Bazaid, Naif K Binsaleh, Heba Barnawi, Bandar Alharbi, Ahmed Alsolami, Samy Selim, Soad K Al Jaouni, Amna A Saddiq, Magdah Ganash, Tarek M Abdelghany, Husam Qanash","doi":"10.1186/s40643-025-00855-y","DOIUrl":"10.1186/s40643-025-00855-y","url":null,"abstract":"<p><p>Natural products of plant origin are being explored as safe alternatives for illness management. Their extraction processes play a crucial role in determining their phytochemical and pharmacological properties. In this context, Euphorbia trigona was extracted using Supercritical Fluid Extraction with CO₂ (SFE-CO₂) at two operating temperatures: 20 °C and 40 °C. Phytochemical characterization was performed via HPLC, along with anti-yeast evaluation using the well diffusion method, anticancer assessment using the MTT assay, wound healing analysis via the scratch assay, and anti-obesity evaluation through the lipase assay of the E. trigona extract. The results indicated that SFE-CO₂ at 40 °C extracted a greater quantity (0.198 g) of E. trigona than SFE-CO₂ at 20 °C (0.156 g). Several compounds, such as rosmarinic acid, gallic acid, daidzein, ellagic acid, naringenin, and ferulic acid, were identified at high concentrations of 10,034.29, 1,800.33, 750.22, 748.11, 462.15, and 207.05 µg/mL, respectively, in the E. trigona extract obtained using SFE-CO₂ at 40 °C, compared to the extract obtained using SFE-CO₂ at 20 °C. High inhibition zones of 24 ± 1.5, 24 ± 0.5, and 23 ± 0.33 mm were recorded against C. albicans, C. tropicalis, and G. candidum, respectively, using the extract from SFE-CO₂ at 40 °C, compared to the inhibition zones of 24 ± 1.5, 24 ± 0.5, and 23 ± 0.33 mm obtained from the extract using SFE-CO₂ at 20 °C. Moreover, the extract from SFE-CO₂ at 40 °C exhibited lower MIC and MFC values against the tested yeasts compared to the efficacy of the extract from SFE-CO₂ at 20 °C. The ultrastructure of the examined yeasts was severely affected by the extract from SFE-CO₂ at 40 °C. A lower IC₅₀ (98.87 ± 1.26 µg/mL) was recorded for the extract from SFE-CO₂ at 40 °C compared to the IC₅₀ (333.87 ± 1.8 µg/mL) of the extract from SFE-CO₂ at 20 °C against cancer cells (A431). The wound closure level was 84.08% using the extract from SFE-CO₂ at 40 °C, while it was 71.27% using the extract from SFE-CO₂ at 20 °C. Lipase was inhibited by the extract obtained via SFE-CO₂ at 40 °C and 20 °C, with IC₅₀ values of 15.77 and 28.14 µg/mL, respectively. Molecular docking indicated that rosmarinic acid is a suitable inhibitor for the tested yeasts.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"28"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities.","authors":"Karnan Ramachandran, Senthil Bakthavatchalam, Shunmuga Vadivu Ramalingam, Ramachandran Vinayagam, Mukeshwaran Ramesh, Sukumaran Marimuthu, Zhi-Hong Wen, Chandramohan Govindasamy, Khalid M Almutairi, Yi-Hao Lo","doi":"10.1186/s40643-025-00868-7","DOIUrl":"10.1186/s40643-025-00868-7","url":null,"abstract":"<p><p>This study investigates the in silico anti-arboviral potential of zoochemicals derived from the methanolic extract of Charybdis natator shell, alongside their larvicidal efficacy against Aedes aegypti 4th instar larvae. Through GC-MS analysis, 27 zoochemicals were identified, demonstrating promising in silico activity against molecular antiviral targets: DENV2 protease (PDB: 6MO1) for anti-dengue, RNA polymerase (PDB: 5U04) for anti-Zika, and nsP2 protease (PDB: 3TRK) for anti-chikungunya. A strong positive correlation (r = 0.726-0.889) in binding affinities (kcal/mol) suggests a consistent inhibitory mechanism across these targets. Furthermore, PASS analysis indicates higher probabilities of activity (Pa) for insecticidal properties compared to antiviral efficacy, highlighting their dual potential as larvicidal agents and antiviral candidates. The methanolic extract of Charybdis natator shell exhibited potent larvicidal activity against Aedes aegypti (LC₅₀ = 81.001 µg/mL) in a dose-dependent manner (R² = 0.968). In silico analysis further elucidated its inhibitory action on key growth regulators of A. aegypti, underscoring its potential to disrupt larval development. These findings highlight the dual utility of C. natator shell extract in vector management and in mitigating the transmission of arboviral diseases such as Dengue, Zika, and Chikungunya. The extract's promise as an eco-friendly, cost-effective source for developing novel insecticidal and antiviral agents merits further exploration.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"29"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phycochemistry and pharmacological significance of filamentous cyanobacterium Spirulina sp.","authors":"Sanjana Sabat, Shuvasree Bej, Surendra Swain, Ajit Kumar Bishoyi, Chita Ranjan Sahoo, Goutam Sabat, Rabindra Nath Padhy","doi":"10.1186/s40643-025-00861-0","DOIUrl":"10.1186/s40643-025-00861-0","url":null,"abstract":"<p><p>The cyanobacterium, Spirulina sp. is a photosynthetic blue-green alga with essential nutrients, vitamins nucleic acids, proteins, carbohydrates, fatty acids and pigments carotenes; and phycocyanins are the significant components having immunomodulatory, anti-inflammatory properties, which are used in food and cosmetics industries. Spirulina sp. can play an important role in human and animal nutrition for potential health benefits due to their phycochemical and pharmaceutical significance. This study highlights antibacterial, antifungal, antiviral, antioxidant, nephroprotective, cardioprotective, anticancer, neuroprotective, anti-aging, anti-inflammatory, and immunomodulatory properties. It highlights anti-anemic, antidiabetic, probiotic, anti-malarial, anti-obesity and weight loss, anti-genotoxicity, anti-thrombic, radioprotective, and detoxifying effects of Spirulina sp. Pharmaceutical studies indicate it may improve heart health and add to the treatment of diabetes, obesity and weight loss. It can play a major role in protecting the environment by recycling wastewater and providing food for humans and animals. Spirulina sp. can supply ingredients for aquaculture and agricultural feeds, pigments, antioxidants, and essential omega-3 oils, among other human health and wellness products. The amino acid of Spirulina is among the greatest qualititavely of any plant, even higher than that of soybean. Furthermore, cyanobacterium Spirulina sp. could be a future antimicrobial drug agent.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"27"},"PeriodicalIF":4.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vinasses valorization into short-chain fatty acids: microbiome robustness against process variations.","authors":"Silvia Greses, Mercedes Llamas, Aboudi Kaoutar, Cristina González-Fernández","doi":"10.1186/s40643-025-00865-w","DOIUrl":"10.1186/s40643-025-00865-w","url":null,"abstract":"<p><p>The valorization of vinasses into short-chain fatty acids (SCFAs) via anaerobic fermentation (AF) is an emerging approach that remains under research. Given the diverse microbial metabolisms simultaneously occurring in AF, the control of operational parameters is essential to avoid process destabilization. To unravel their effect, the novelty of this investigation relied on the evaluation of the robustness of AF process against operational perturbation deliberately set (i.e. hydraulic retention time (HRT) and temperature increase). Regardless the applied perturbation, similar yields (0.5-0.6 g COD-SCFAs/g VS_in) were attained. However, the selected perturbations exerted an effect on microbiome development. Whereas the temperature increase mediated a 49.70% microbiome dissimilarity, only a 21.91% dissimilarity was caused by the HRT increase. Microbial analysis revealed Clostridiales, Prevotella and Megasphaera as key bacteria in vinasses degradation. The similar bioconversion obtained despite the different microbiomes developed after each perturbation suggested a functional redundancy highlighting the AF robustness. These findings evidenced AF as a feasible biotechnology to further valorize vinasse into SCFAs, demonstrating the process stability against common perturbations that might be encountered at industrial scale.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"26"},"PeriodicalIF":4.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photofermentative production of poly-β-hydroxybutyrate (PHB) by purple non-sulfur bacteria using olive oil by-products.","authors":"Gianmarco Mugnai, Luca Bernabò, Giulia Daly, Elisa Corneli, Alessandra Adessi","doi":"10.1186/s40643-025-00856-x","DOIUrl":"10.1186/s40643-025-00856-x","url":null,"abstract":"<p><p>This study evaluated the ability of six purple non-sulfur bacteria (PNSB) to convert olive oil by-products into poly-β-hydroxybutyrate (PHB). Strains were first independently cultivated in synthetic media with different carbon sources (acetic, lactic and malic acid) to assess their physiology and PHB production. Subsequently, their growth and PHB production using ingested pâté olive cake (IPOC) as a substrate were investigated. Transmission electron microscopy (TEM) observations were conducted on strains cultivated on IPOC to investigate their cell morphologies and inclusion bodies presence and size. Rhodopseudomonas palustris strains accumulated up to 6.8% w PHB/w cells with acetate and 0.86% w PHB/w cells with a daily productivity of 0.54 mg PHB L⁻¹ culture d⁻¹ on IPOC. In contrast, Cereibacter johrii and Cereibacter sphaeroides reached 58.64% w PHB/w cells and 65.45% w PHB/w cells with acetate, respectively, while C. sphaeroides achieved 21.48% w PHB/w cells and a daily productivity of 10.85 mg PHB L⁻¹ culture d⁻¹ when cultivated on IPOC. All strains exhibited growth and PHB accumulation in both synthetic media and IPOC substrate. Specifically, R. palustris strains 42OL, AV33 and CGA009 displayed growth capability in all substrates, while C. johrii strains 9Cis and PISA 7, and C. sphaeroides F17 showed promising PHB synthesis capabilities. TEM observations revealed that R. palustris strains, with smaller cell and inclusion body sizes, exhibited lower PHB accumulations, while C. johrii and C. sphaeroides strains, characterized by larger cells and inclusion bodies, demonstrated higher PHB production, recognizing them as promising candidates for PHB production using olive oil by-products. Further investigations under laboratory-scale conditions will be necessary to optimize operating parameters and develop integrated strategies for simultaneous PHB synthesis and the co-production of value-added products, thereby enhancing the economic feasibility of the process within a biorefinery framework.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"25"},"PeriodicalIF":4.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}