Bioresources and Bioprocessing最新文献

{"title":"Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water","authors":"Amer Jamal Hashmat, Muhammad Afzal, Samina Iqbal, Imran Amin, Carlos Alberto Arias, Hans Brix, Imran Zafar, Sania Riaz, Rizwan ur Rehman, Ahmad Mohammad Salamatullah, Gezahign Fentahun Wondmie, Mohammed Bourhia","doi":"10.1186/s40643-024-00757-5","DOIUrl":"https://doi.org/10.1186/s40643-024-00757-5","url":null,"abstract":"<p>The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) <i>alkB</i> gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of <i>Typha latifolia</i> and <i>Cyperus laevigatus</i> and inoculated with the bacterial consortium (<i>Pseudomonas putida</i> TYRI39, <i>Acinetobacter junii</i> TYRH47, <i>Acinetobacter</i> sp. CYRH17, <i>Pseudomonas</i> sp. CYSI27, and <i>Pseudomonas</i> sp. TYRH42). The mesocosms were provided with nutrients (20 mg l^− 1 N, 2.6 mg l^− 1 P, and 16.4 mg l^− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l^− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having <i>C. laevigatus</i>, bacteria, and NSA, and it is correlated with the copy numbers of <i>alkB</i> and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"9 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A straightforward chemobiocatalytic route for one-pot valorization of glucose into 2,5-bis(hydroxymethyl)furan","authors":"Xuan-Ping Liao, Qian Wu, Min-Hua Zong, Ning Li","doi":"10.1186/s40643-024-00758-4","DOIUrl":"https://doi.org/10.1186/s40643-024-00758-4","url":null,"abstract":"<p>Direct conversion of inexpensive biomass into value-added chemicals via furanic platform molecules is highly attractive. In this work, we present a straightforward chemobiocatalytic route for glucose valorization into 2,5-bis(hydroxymethyl)furan (BHMF) in one pot, with no purification of the intermediate 5-hydroxymethylfurfural (HMF). Six candidate alcohol dehydrogenase (ADH) genes were located from <i>Meyerozyma guilliermondii</i> SC1103, based on comparative transcriptome analysis and real-time quantitative polymerase chain reaction. An ADH (<i>Mg</i>ADH1) was identified upon evaluation of catalytic performances of recombinant <i>Saccharomyces cerevisiae</i> harboring candidate ADHs in HMF reduction. Soluble expression of the enzyme in <i>S. cerevisiae</i> was greatly enhanced by its codon optimization, leading to improved HMF tolerance (up to 400 mM). In a fed-batch process, the desired product of approximately 473 mM (60.5 g/L) was produced within 30 h by recombinant <i>S. cerevisiae</i>_<i>Mg</i>ADH1. A chemobiocatalytic route toward BHMF was constructed by merging CaCl₂-mediated isomerization and dehydration with biocatalytic reduction with an overall yield of approximately 42%, starting from glucose. This work may pave the way for green manufacture of valuable biobased chemicals.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"122 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the biosynthesis of polyunsaturated fatty acids by Rhodotorula mucilaginosa and Lodderomyces elongisporus","authors":"Amera A. Abaza, Yousseria M. Shetaia, Noha M. Sorour, Ashraf S. A. El-Sayed, Ashraf F. El-Baz","doi":"10.1186/s40643-024-00755-7","DOIUrl":"https://doi.org/10.1186/s40643-024-00755-7","url":null,"abstract":"<p>Single-cell oils (SCO) produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids. However, the accumulation of oils varies substantially between yeast spp. Consequently, identifying well-suited producers with a high innate capacity for lipids biosynthesis is paramount. Equally important is optimizing culturing and processing conditions to realize the total lipids production potential of selected strains. The marine <i>Rhodotorula mucilaginosa</i> and <i>Lodderomyces elongisporus</i> yeast were investigated to explore their potential for polyunsaturated fatty acids (PUFAs) production on high glucose media (HGM) using two-stage culture mode. Both strains accumulated &gt; 20% (w/w) of their dry cell weight as lipids when grown on HGM using a two-stage culture system. Both yeast isolates exhibited a maximal lipid/biomass coefficient (Y_L/X) of 0.58–0.66 mg/mg at 7 °C and 0.49–0.53 mg/mg at 26 °C when grown on 8% glucose and produced monounsaturated and PUFAs similar to that of Menhaden and Salmon marine oils. For the first time, significant amounts of Eicosapentaenoic acid (19%) and Eicosadienoic acid (19.6%) were produced by <i>L. elongisporus</i> and <i>R. mucilaginosa</i>, respectively. Thus, the SCO derived from these wild strains possesses significant potential as a substitute source for the industrial-scale production of long-chain PUFAs, making them a promising contender in the market.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"57 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris","authors":"Junyi Li, Manli Yang, Fengguang Zhao, Yaping Zhang, Shuangyan Han","doi":"10.1186/s40643-024-00752-w","DOIUrl":"https://doi.org/10.1186/s40643-024-00752-w","url":null,"abstract":"<p>Pectin lyase (PMGL) is an industrially important enzyme with widespread applications in the food, paper, and textile industries, owing to its capacity for direct degradation of highly esterified pectin. In this study, PMGL-Ba derived from <i>Bacillus licheniformis</i> underwent mining and heterologous expression in <i>P. pastoris.</i> Furthermore, diverse strategies, encompassing the optimization of expression cassette components, elevation of gene dosage, and co-expression of chaperone factors, were employed to augment PMGL-Ba production in <i>P. pastoris</i>. The signaling peptide OST1-pre-α-MF-pro and promoter AOX1 were finally selected as expression elements. By overexpressing the transcription factor Hac1p in conjunction with a two-copy PMGL-Ba setup, a strain yielding high PMGL-Ba production was achieved. In shake flask fermentation lasting 144 h, the total protein concentration reached 1.81 g/L, and the enzyme activity reached 1821.36 U/mL. For further scale up production, high-density fermentation transpired in a 5 L fermenter for 72 h. Remarkably, the total protein concentration increased to 12.49 g/L, and the enzyme activity reached an impressive 12668.12 U/mL. The successful heterologous and efficient expression of PMGL-Ba not only furnishes a valuable biological enzyme for industrial applications but also contributes to cost reduction in the utilization of biological enzymes in industrial applications.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"204 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant","authors":"Leticia F. Ngum, Y. Matsushita, Samir F. El-Mashtoly, Ahmed M. R. Fath El-Bab, Ahmed L Abdel-Mawgood","doi":"10.1186/s40643-024-00746-8","DOIUrl":"https://doi.org/10.1186/s40643-024-00746-8","url":null,"abstract":"<p>Cell separation using microfluidics has become an effective method to isolate biological contaminants from bodily fluids and cell cultures, such as isolating bacteria contaminants from microalgae cultures and isolating bacteria contaminants from white blood cells. In this study, bacterial cells were used as a model contaminant in microalgae culture in a passive microfluidics device, which relies on hydrodynamic forces to demonstrate the separation of microalgae from bacteria contaminants in U and W-shaped cross-section spiral microchannel fabricated by defocusing CO₂ laser ablation. At a flow rate of 0.7 ml/min in the presence of glycine as bacteria chemoattractant, the spiral microfluidics devices with U and W-shaped cross-sections were able to isolate microalgae (<i>Desmodesmus</i> sp.) from bacteria (<i>E. coli</i>) with a high separation efficiency of 92% and 96% respectively. At the same flow rate, in the absence of glycine, the separation efficiency of microalgae for U- and W-shaped cross-sections was 91% and 96%, respectively. It was found that the spiral microchannel device with a W-shaped cross-section with a barrier in the center of the channel showed significantly higher separation efficiency. Spiral microchannel chips with U- or W-shaped cross-sections were easy to fabricate and exhibited high throughput. With these advantages, these devices could be widely applicable to other cell separation applications, such as separating circulating tumor cells from blood.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"298 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of hospital wastewater treatment using locally isolated Chlorella sp. LH2 from cocoon wastewater","authors":"Tu Thi Anh Le, Truong Nguyen","doi":"10.1186/s40643-024-00748-6","DOIUrl":"https://doi.org/10.1186/s40643-024-00748-6","url":null,"abstract":"<p><i>Chlorella</i> sp. is able to grow and transform inorganic and organic contaminants in wastewater to create biomass. In the present study, <i>Chlorella</i> sp. LH2 isolated from cocoon wastewater was able to thrive in hospital wastewater, then remove nutrients and eliminate <i>E. coli</i> ATCC 8739. The results indicated that optimal cultivation conditions of <i>Chlorella</i> sp. LH2 in hospital wastewater were pH of 8, light:dark cycle of 16:8 at 30^oC. The inhibitory effect of chlorination on algae growth was accompanied with the chlorine concentration. BOD₅:COD ratio of 0.77 indicated biodegradability of hospital wastewater. The untreated and treated wastewatee samples were collected to investigated the nutrient removal efficiency after 10 days. Untreated and treated results were192 ± 8.62 mg/l 23.91 ± 2.19 mg/l for BOD₅; 245 ± 9.15 mg/l and 47.31 ± 5.71 mg/l for COD. The treated value met the required standards for hospital wastewater treatment. The removal efficiency total nitrogen and total phosphorus were 68.64% and 64.44% after 10 days, respectively. Elimination of <i>E. coli</i> ATCC 8739 after 7 days by <i>Chlorella</i> sp. LH2 was 88.92%. The results of this study suggest the nutrients and pathogens removal potential of <i>Chlorella</i> sp. LH2 in hospital wastewater for further practical applications.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"2018 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of adaptive laboratory evolution of engineered Escherichia coli in acetate on the biosynthesis of succinic acid from glucose in two-stage cultivation","authors":"Jiaping Jiang, Yuanchan Luo, Peng Fei, Zhengtong Zhu, Jing Peng, Juefeng Lu, Du Zhu, Hui Wu","doi":"10.1186/s40643-024-00749-5","DOIUrl":"https://doi.org/10.1186/s40643-024-00749-5","url":null,"abstract":"<p><i>Escherichia coli</i> MLB (MG1655 Δ<i>pflB</i> Δ<i>ldhA</i>), which can hardly grow on glucose with little succinate accumulation under anaerobic conditions. Two-stage fermentation is a fermentation in which the first stage is used for cell growth and the second stage is used for product production. The ability of glucose consumption and succinate production of MLB under anaerobic conditions can be improved significantly by using acetate as the solo carbon source under aerobic condition during the two-stage fermentation. Then, the adaptive laboratory evolution (ALE) of growing on acetate was applied here. We assumed that the activities of succinate production related enzymes might be further improved in this study. <i>E. coli</i> MLB46-05 evolved from MLB and it had an improved growth phenotype on acetate. Interestingly, in MLB46-05, the yield and tolerance of succinic acid in the anaerobic condition of two-stage fermentation were improved significantly. According to transcriptome analysis, upregulation of the glyoxylate cycle and the activity of stress regulatory factors are the possible reasons for the elevated yield. And the increased tolerance to acetate made it more tolerant to high concentrations of glucose and succinate. Finally, strain MLB46-05 produced 111 g/L of succinic acid with a product yield of 0.74 g/g glucose.</p><h3 data-test=\"abstract-sub-heading\">Synopsis</h3>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"298 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910","authors":"Xin Fu, Kexin Lin, Xiaodong Zhang, Zhiyong Guo, Lixin Kang, Aitao Li","doi":"10.1186/s40643-024-00751-x","DOIUrl":"https://doi.org/10.1186/s40643-024-00751-x","url":null,"abstract":"<p>Unspecific peroxygenases (UPOs) are glycosylated enzymes that provide an efficient method for oxyfunctionalizing a variety of substrates using only hydrogen peroxide (H₂O₂) as the oxygen donor. However, their poor heterologous expression has hindered their practical application. Here, a novel UPO from <i>Marasmius fiardii</i> PR910 (<i>Mfi</i>UPO) was identified and heterologously expressed in <i>Pichia pastoris</i>. By employing a two-copy expression cassette, the protein titer reached 1.18 g L⁻¹ in a 5 L bioreactor, marking the highest record. The glycoprotein r<i>Mfi</i>UPO exhibited a smeared band in the 40 to 55 kDa range and demonstrated hydroxylation, epoxidation and alcohol oxidation. Moreover, the peroxidative activity was enhanced by 150% after exposure to 50% (v/v) acetone for 40 h. A semi-preparative production of 4-OH-β-ionone on a 100 mL scale resulted in a 54.2% isolated yield with 95% purity. With its high expression level, r<i>Mfi</i>UPO is a promising candidate as an excellent parental template for enhancing desirable traits such as increased stability and selectivity through directed evolution, thereby meeting the necessary criteria for practical application.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"14 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the biosynthesis and industrial biotechnology of Gamma-amino butyric acid","authors":"","doi":"10.1186/s40643-024-00747-7","DOIUrl":"https://doi.org/10.1186/s40643-024-00747-7","url":null,"abstract":"<h3>Abstract</h3> <p>GABA (Gamma-aminobutyric acid), a crucial neurotransmitter in the central nervous system, has gained significant attention in recent years due to its extensive benefits for human health. The review focused on recent advances in the biosynthesis and production of GABA. To begin with, the investigation evaluates GABA-producing strains and metabolic pathways, focusing on microbial sources such as Lactic Acid Bacteria, <em>Escherichia coli</em>, and <em>Corynebacterium glutamicum</em>. The metabolic pathways of GABA are elaborated upon, including the GABA shunt and critical enzymes involved in its synthesis. Next, strategies to enhance microbial GABA production are discussed, including optimization of fermentation factors, different fermentation methods such as co-culture strategy and two-step fermentation, and modification of the GABA metabolic pathway. The review also explores methods for determining glutamate (Glu) and GABA levels, emphasizing the importance of accurate quantification. Furthermore, a comprehensive market analysis and prospects are provided, highlighting current trends, potential applications, and challenges in the GABA industry. Overall, this review serves as a valuable resource for researchers and industrialists working on GABA advancements, focusing on its efficient synthesis processes and various applications, and providing novel ideas and approaches to improve GABA yield and quality.</p> <span> <h3>Graphical Abstract</h3> <p> <span> <span> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/40643_2024_747_Figa_HTML.png\"/> </span> </span></p> </span>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"130 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced enzymatic sugar production from corn stover by combination of water extraction and glycerol-assisted instant catapult steam explosion","authors":"Fengqin Wang, Hongli Dong, Weiwei Yu, Yinling Gao, Guotao Mao, Yanxia An, Hui Xie, Andong Song, Zhanying Zhang","doi":"10.1186/s40643-024-00739-7","DOIUrl":"https://doi.org/10.1186/s40643-024-00739-7","url":null,"abstract":"<p>Glycerol-assisted instant catapult steam explosion (ICSE) of lignocellulose is an effective pretreatment method for enhancing sugar production compared to glycerol-free ICSE. In this study, glycerol-assisted ICSE of corn stover was studied in order to understand the reaction mechanisms and further optimize the process. Results showed that water extraction of corn stover prior to ICSE reduced pseudo-lignin formation. The combination of water extraction and glycerol-assisted ICSE led to the formation of lignin with a lower molecular weight (Mw) of 2851 g/mol than 3521 g/mole of that from the combination of water extraction and glycerol-free ICSE. ¹H-¹³C NMR analysis revealed that glycerol likely reacted with lignin carboxylic OHs through esterification while etherification of aliphatic OHs was not observed in ICSE. These lignin analyses indicated that glycerol protected lignin from condensation/repolymerization during glycerol-assisted ICSE. Enzymatic hydrolysis results showed that without water extraction increasing glycerol usage from 0.2 kg/kg stover to 0.4 kg/kg stover improved glucan digestibility to 78% but further increase to 0.5 kg/kg stover reduced glucan digestibility. In addition, at the glycerol usage of 0.2–0.4 kg/kg stover, washing of pretreated stover for removal of glycerol and other biomass-derived compounds did not improve glucan digestibility compared to unwashed ones. Combination of water extraction and glycerol-assisted ICSE led to a high glucan digestibility of 89.7% and a total glucose yield of 25.5 g glucose/100 g stover, which were 30.1% and 7.5 g/100 g stover higher than those derived from glycerol-free ICSE of stover, respectively. Since glycerol is a low-cost carbon source, the resulting enzymatic hydrolysate that contained both glucose and glycerol may be directly used to produce bioproducts by microbial fermentation.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"15 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140125718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}