Bioresources and Bioprocessing最新文献

{"title":"Optimization of cultivation strategies for production of recombinant human papillomavirus type 58 major capsid protein L1 in Hansenula polymorpha.","authors":"Natsima Kopitak, Wichittra Phimsen, Kittipol Sripui, Auntika Khunsom, Natchanon Pongsuwichedsak, Tatpong Boontawon, Thantawat Theeranan, Chuenchit Boonchird, Thunyarat Pongtharangkul","doi":"10.1186/s40643-026-01059-8","DOIUrl":"10.1186/s40643-026-01059-8","url":null,"abstract":"<p><p>Human papillomaviruses (HPVs) are a major cause of cervical cancer, which ranks fourth among cancers in women worldwide. A prophylactic vaccine composed of virus-like particles (VLPs) formed by the major capsid protein L1 of human papillomavirus (HPV) effectively prevents HPV infection but provides limited cross-protection against other HPV subtypes, highlighting the importance of multivalent prophylactic vaccines. In this study, cultivation conditions for recombinant HPV58 L1 protein production in the yeast Hansenula polymorpha were optimized to enhance growth and the volumetric yield of L1 protein. The results indicated that induction with methanol (at 1% v/v) was necessary for HPV58 L1 production in SYN6 medium, whereas a cultivation temperature of 37 °C was optimal for growth and production of HPV58 L1 protein. Subsequently, SYN6 medium supplemented with 10 g/L of either Hy-Express™ System II or HySoy was evaluated in batch and fed-batch bioreactor cultivations. Fed-batch cultivation with HySoy supplementation under a constant feeding rate achieved an OD₆₆₀ of 117 (26.18 g-CDW/L), a volumetric L1 yield of 312 mg/L, and a productivity of 4.7 mg/L/h, which were significantly higher than those obtained with the control SYN6 medium (92 mg/L; 2.2 mg/L/h). These findings demonstrate that fed-batch cultivation with HySoy supplementation offers a practical and efficient strategy for HPV58 L1 production at the bioreactor scale.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13201806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MyBioScope: a new frontier in gut microbiome and health research.","authors":"Kristina Žukauskaitė, Angela Horvath, Selina Tripolt, Hansjörg Habisch, Tobias Madl, Christian Pacher-Deutsch, Maximilian Nepel, Irina Balazs, Vanessa Stadlbauer","doi":"10.1186/s40643-026-01044-1","DOIUrl":"10.1186/s40643-026-01044-1","url":null,"abstract":"<p><p>Disruptions in the gut microbiome are linked to various diseases, but their roles in conditions such as age-related muscle loss (sarcopenia) and drug-induced microbial changes remain poorly understood. To address this gap, MyBioScope, a novel in vitro model using the DASbox® mini bioreactor system and human stool samples, was developed to simulate the anaerobic environment of the gastrointestinal (GI) tract. Bioreactors containing 120–200 mL of cultivation media were inoculated with stool slurry, stabilized over 24 h, and maintained with a customizable feeding protocol for multi-day experiments. Samples were analyzed using 16S rRNA gene sequencing, quantitative PCR, and metabolomics. Four pilot studies were conducted to validate the platform and model specific disease states, including proton pump inhibitor-induced GI tract oralization and microbiome alterations associated with sarcopenia. The workflow incorporated an anaerobic stool collection kit for user-friendly, room-temperature sample transport and storage. Our results demonstrated consistent microbial community structure and metabolic activity within disease-mimicking conditions. MyBioScope enabled reproducible, controlled studies of gut microbial dynamics and provided a scalable tool for investigating disease-specific microbiome changes. This platform may support translational efforts to integrate microbiome insights into clinical research, therapeutic development, and personalized medicine. In conclusion, this novel bioreactor-based in vitro model, MyBioScope, shows strong potential for in-depth exploration of disease-specific microbiomes and can facilitate new ways for integrating the knowledge of the microbiome’s impact on human health and disease into clinical practice.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13198590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure-dependent supercritical CO₂ extraction of Heliotropium arbainense: phytochemical enrichment and enhanced antimicrobial, anti-inflammatory, and pro-apoptotic activities.","authors":"Samy Selim, Samiah Hamad Al-Mijalli, Souzan Mohammed Kafy, Mohammed H Alruhaili, Hattan S Gattan, Mutasem S Almehayawi, Mohammed Aladhadh, Emad M Abdallah, Mohamed A Amin","doi":"10.1186/s40643-026-01080-x","DOIUrl":"10.1186/s40643-026-01080-x","url":null,"abstract":"<p><p>Supercritical CO₂ extraction was applied to Heliotropium arbainense to evaluate how extraction pressure influences phytochemical composition and biological activity. Extractions of phenolic and flavonoid were performed at two pressures (200 and 550 bar), by using Supercritical CO₂. Phenolic and flavonoid compounds, were determined by HPLC analysis. Higher pressure extraction (550 bar) resulted in a greater extraction yield and a marked enrichment of phenolic and flavonoid compounds. Gallic acid (1496.01 µg/g) was the most prevalent component under SFE 1, followed by catechin (859.51 µg/g), rutin (824.55 µg/g), and coumaric acid (559.93 µg/g). Both extracts exhibited broad antimicrobial activity against Gram-positive and Gram-negative bacteria as well as Candida albicans, with consistently lower MIC and MBC/MFC values observed for the high-pressure extract. Cytotoxic evaluation against SKOV3 ovarian cancer cells revealed a clear dose-dependent reduction in cell viability. The calculated IC₅₀ values further confirm the greater cytotoxic potency of extract at 550 bar, with a lower IC₅₀ (153.04 ± 0.4 µg/mL) compared to extract at 200 bar (183.18 ± 2.29 µg/mL) against SKOV3 cells. However, flow cytometry revealed that extract at 200 bar produced slightly higher early apoptosis (43.79% vs. 43.39%) and greater necrosis (21.83% vs. 13.29%) in SKOV3 ovarian cells overall. Anti-inflammatory activity, assessed by protein denaturation inhibition, was observed for both extracts, with the lower-pressure extract showing slightly stronger inhibitory efficiency. Pressure-dependent supercritical CO₂ extraction significantly influenced the phytochemical richness and biological performance of H. arbainense. These findings highlight the importance of extraction conditions in maximizing the functional potential of plant-derived bioactive compounds and support further investigation of H. arbainense as a source of biologically active phenolics.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of brewer's spent grains by Pleurotus ostreatus: process optimization by response surface methodology.","authors":"Victoria-Luisa Hrazdil, Paula Hallmann, Josephine Dresler, Marco A Fraatz, Holger Zorn","doi":"10.1186/s40643-026-01018-3","DOIUrl":"10.1186/s40643-026-01018-3","url":null,"abstract":"<p><p>To achieve high-value utilization of brewer's spent grains and to produce protein-rich food ingredients, brewer's spent grains were upcycled by a liquid fungal fermentation and the fermentation conditions were optimized. Brewer's spent grains (BSG) represent the most abundant by-product of the brewing industry. Although BSG are food-grade, their direct use in food is limited because of sensory changes of the products. In this study, screening experiments revealed that black beer spent grains could be efficiently upcycled by submerged fermentation with the edible fungus Pleurotus ostreatus. The conditions of the fermentation of BSG with P. ostreatus were optimized using response surface methodology, including the parameters substrate concentration, inoculum volume, initial pH value, and temperature. As no separation between BSG and mycelium was possible after the fermentation, ergosterol was used as a biomarker to determine the fungal growth. As optimum conditions, a BSG concentration of 17 g L^- 1 dry matter, an inoculum volume of 4.4% (v/v), an initial pH of 9.3 and a temperature of 30 °C were identified. The fermentation with P. ostreatus increased the true protein content of the biomass compared to the spent grains and reduced the total fat content. The biological value was increased from 88 to 94 (reference standard is whole chicken egg with a biological value of 100). Tryptophan and lysine were limiting in the non-fermented spent grains, while after fermentation, the chemical score of lysine increased from 78 to 92, and tryptophan wasn't limiting anymore. Fermentation by P. ostreatus enhanced the nutritional value of BSG, and the fermentation conditions were optimized by using response surface methodology.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13176399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of pretreatment and enzymatic hydrolysis using commercial and isolated bacterial enzyme cocktail for bioethanol production from corn husk through yeast co-culture batch fermentation.","authors":"Barsha Samantaray, Rashmi Ranjan Mishra, Sonali Mohapatra, Sakti Rath, Bikash Chandra Behera, Hrudayanath Thatoi","doi":"10.1186/s40643-026-01062-z","DOIUrl":"10.1186/s40643-026-01062-z","url":null,"abstract":"<p><p>This study assesses the impact of physicochemical pretreatment, enzymatic hydrolysis, and co-culture fermentation strategies on bioethanol production from corn husk biomass (CHB). Under optimal alkali pretreatment conditions (1.75% alkali, 4.0 g substrate concentration, 120 °C, 10 h), 35% lignin removal was achieved, with 48% cellulose and 39% hemicellulose recovery. In contrast, acid pretreatment resulted in 30% lignin removal, 45% cellulose recovery, and 34% hemicellulose recovery, showing lower efficiency than alkali pretreatment. During ultrasonication alkali pretreatment enhanced cellulose and hemicellulose exposure up to 51 and 46% and delignification up to 49%. Enzymatic hydrolysis of pretreated corn husk biomass was performed using commercial enzymes [Celluclast 1.5 L (700 EGU or 854 U mL^-1) and Viscozyme (13.4 FBG/mL)] and isolated bacterial enzymes, including cellulase from Bacillus licheniformis (9.3 ± 0.3 U mL^-1) and xylanase from Enterobacter asburiae PQ396173 (7.0 ± 0.4 U mL^-1). The developed enzyme cocktail in ratio 3:2:3:1 (v/v; U mL^-1) (Celluclast: Viscozyme: native cellulase: native xylanse) using a cocktail of native and commercial enzymes, yielded total reducing sugar of 740 mg g^-1 glucose and 54.6 mg g^-1 xylose. Fermentation of hydrolysate prepared with commercial enzymes using monoculture of Saccharomyces cerevisiae and Pichia pastoris yielded 17.6 g L^-1 and 12.2 g L^-1 bioethanol separately. Co-cultured yeasts produced 26.8 g L^-1 ethanol at 96 h of incubation, exceeding monoculture yields. The fermentation with integration of commercial and isolated bacterial enzyme cocktails yielded the highest bioethanol output of 37.3 g L^-1 at 96 h incubation, indicating that enzymatic saccharification with a combination of commercial and native enzyme cocktails results in maximum bioethanol production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13172165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147926176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular encapsulation of indigo with β-cyclodextrin and Hydroxypropyl-β-cyclodextrin: a sustainable strategy for enhanced solubility, stability, and reducing agent-free dyeing processes.","authors":"Koijam Monica Devi, Nimya Krishnan, Chan-Seo Yeo, Kwon-Young Choi","doi":"10.1186/s40643-026-01065-w","DOIUrl":"10.1186/s40643-026-01065-w","url":null,"abstract":"<p><p>Indigo, a ubiquitous vat dye in denim manufacturing, is characterized by its exceptionally low aqueous solubility, necessitating chemical reduction to its leuco form using hazardous agents such as sodium dithionite. This conventional process yields sulfite, sulfate, and sulfide byproducts, leading to significant environmental and toxicological concerns. To address these limitations, this study synthesized 1:1 molar ratio inclusion complexes of indigo with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) to enhance solubility and stability without chemical intervention. The formation of these complexes was rigorously validated through UV-Vis, FT-IR, PXRD, TEM, and ¹H-NMR spectroscopy. Notably, the aqueous solubility of indigo was enhanced 3.58-fold and 5.55-fold for the β-CD and HP-β-CD complexes, respectively. Furthermore, both complexes demonstrated superior thermal and photostability, with HP-β-CD exhibiting the most pronounced effects. This cyclodextrin-assisted solubilization was successfully extended to naturally extracted Jeju Indigo, underscoring its broad applicability. Our findings suggest that cyclodextrin-based encapsulation offers a sustainable and effective alternative to conventional reduction-oxidation dyeing processes.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13172062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147926172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanol-based biosynthesis of p-coumaric acid by engineered Pichia pastoris.","authors":"Mengyuan Chen, Jiayu Fang, Shuxian Wang, Guoxia Liu, Yanping Zhang, Yin Li, Kaizhi Jia, Taicheng Zhu","doi":"10.1186/s40643-026-01068-7","DOIUrl":"10.1186/s40643-026-01068-7","url":null,"abstract":"<p><p>p-Coumaric acid (p-CA) is a key aromatic precursor for the biosynthesis of flavonoids, stilbenoids, and other high-value phenylpropanoids. While microbial production of p-CA typically relies on sugar-based substrates, methanol offers a sustainable and cost-effective alternative, though its use for aromatic biosynthesis remains unexplored. Here, we report the first de novo production of p-CA from methanol using engineered methylotrophic yeast Pichia pastoris. Through heterologous expression of a tyrosine ammonia-lyase and implementing a balanced push-pull strategy in the shikimate pathway using feedback-resistant variants of DAHP synthase (ARO4) and chorismate mutase (ARO7), carbon flux from methanol-derived C3 and C4 precursors was effectively redirected toward aromatic biosynthesis. Shake-flask studies revealed strong gene-dosage-dependent p-CA production, but strains with high-copy numbers suffered metabolic burden under high-density fermentation. Fed-batch bioreactor cultivation demonstrated that a moderate-copy strain achieved the highest titer of 704 ± 6 mg/L, outperforming high-copy variants in robustness and scalability. This study establishes P. pastoris as a promising chassis for methanol-based aromatic production and highlights the critical trade-off between pathway amplification and cellular fitness in C1 biomanufacturing.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13172077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147926186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proximity-enhanced co-immobilized enzyme cascade for efficient bioconversion of nicotine to 3-succinoylpyridine.","authors":"Yelong Wang, Jiandong Zhang, Hongjing Yang, Jinbin Wei, Kai Song, Shan Li, Laiwei Shen, Guangyu Yang, Mohamed Yassin Ali, Zhen Wang, Yong Zhang, Yuzhen Wang","doi":"10.1186/s40643-026-01066-9","DOIUrl":"10.1186/s40643-026-01066-9","url":null,"abstract":"<p><p>Tobacco waste, rich in nicotine, is both an environmental burden and a potential feedstock for high-value chemicals. Here, we developed a proximity-enhanced co-immobilized multi-enzyme cascade that efficiently converts the nicotine into a pharmaceutical intermediate 3-succinoylpyridine (SP). The cascade system comprises the nicotine oxidoreductase NicA2 V321 (NicAm), pseudooxynicotine amine oxidase (Pnao), and 3-succinoylsemialdehyde-pyridine dehydrogenase (Sapd) for sequential nicotine conversion, coupled with an aldehyde-ketone reductase (AKR) module for in situ NADP⁺ regeneration. To improve nicotine-to-SP conversion and facilitate multi-enzyme recycling, a SpyCatcher/SpyTag self-assembled cofactor regeneration enzyme scaffold was adopted, in conjunction with AviTag-BirA-mediated biotinylation for cascade enzyme site-specific co-immobilization on streptavidin-coated supports. This proximity-enhanced design promoted efficient cofactor cycling and boosted the nicotine-to-SP conversion to approximately 63%, much higher than the 42.7% achieved by free enzymes. The co-immobilized system also showed improved pH and thermal stability, retaining over 60% of its initial activity after eight reuse cycles. This modular biocatalytic strategy provides a green and promising route for the valorization of nicotine-rich tobacco waste.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13172129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147926251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of volatile fatty acids through microbial conversion of waste sludges: influence of substrates.","authors":"Lei Liu, Jenni Salminen, Taina Lundell, Martin Romantschuk, Merja Hannele Kontro","doi":"10.1186/s40643-026-01049-w","DOIUrl":"10.1186/s40643-026-01049-w","url":null,"abstract":"<p><p>Inadequate sludge treatment poses a significant risk of environmental pollution. To reduce pollution, utilizing waste sludge as an energy source offers a sustainable solution to mitigate pollution. Since sludges contain abundant organics, they are expected to produce more valuable organics, such as volatile fatty acids (VFAs). Although sludge fermentation has been widely studied, direct yields of VFA production from different sludge types are still limited, particularly for sludge materials originating from Finnish wastewater treatment plants. In this study, laboratory-scale bioreactors were built to examine VFA production from digester feed sludge, digested sludge, and ammonia removal sludge, which were collected from local wastewater treatment plants. Among the tested substrates, digested feed sludge resulted in the highest VFA yield, reaching 171.6 ± 6.3 mg/g volatile solids after 11 days of incubation, which indicated its superior potential for VFA generation. In contrast, bioreactors fed with digested sludge or ammonia removal sludge showed no clear increasing trend in VFA production. The addition of sawdust led to lower overall VFA yields, approximately half of those obtained with digested feed sludge alone, and increasing the sludge proportion did not result in further yield enhancement. Acetic acid was the dominant VFA in all bioreactors, accounting for 61.2-97.9% of total VFAs. Microbial community analysis indicated the prevalence of phyla Bacillota and Pseudomonadota, with Lactobacillus being relatively abundant in bioreactors exhibiting higher VFA production. These findings suggest that sludge type plays an important role in determining VFA production performance and may support more informed selection of sludge substrates for VFA-oriented sludge valorisation.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.","authors":"Shreya S Shettar, Zabin K Bagewadi, Mohammed Alasmary, Basheerahmed Abdulaziz Mannasaheb, Ibrahim Ahmed Shaikh, Aejaz Abdullatif Khan","doi":"10.1186/s40643-026-01048-x","DOIUrl":"10.1186/s40643-026-01048-x","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}