Bioresources and Bioprocessing最新文献

{"title":"Correction: The antioxidant, antidiabetic, and antihyperlipidemic efects of the polyphenolic extract from Salvia Blancoana subsp. Mesatlantica on induced diabetes in rats.","authors":"Souad Maache, Hassan Laaroussi, Najoua Soulo, Ghizlane Nouioura, Nabil Boucetta, Mohammed Bouslamti, Hamza Saghrouchni, Yousef A Bin Jardan, Samir Ibenmoussa, Mohammed Bourhia, Badiaa Lyoussi, Ilham Elarabi","doi":"10.1186/s40643-024-00780-6","DOIUrl":"10.1186/s40643-024-00780-6","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"71"},"PeriodicalIF":4.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11258112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-immobilization of amine dehydrogenase and glucose dehydrogenase for the biosynthesis of (S)-2-aminobutan-1-ol in continuous flow.","authors":"Pengcheng Xie, Jin Lan, Jingshuan Zhou, Zhun Hu, Jiandong Cui, Ge Qu, Bo Yuan, Zhoutong Sun","doi":"10.1186/s40643-024-00786-0","DOIUrl":"10.1186/s40643-024-00786-0","url":null,"abstract":"<p><p>Reductive amination by amine dehydrogenases is a green and sustainable process that produces only water as the by-product. In this study, a continuous flow process was designed utilizing a packed bed reactor filled with co-immobilized amine dehydrogenase wh84 and glucose dehydrogenase for the highly efficient biocatalytic synthesis of chiral amino alcohols. The immobilized amine dehydrogenase wh84 exhibited better thermo-, pH and solvent stability with high activity recovery. (S)-2-aminobutan-1-ol was produced in up to 99% conversion and 99% ee in the continuous flow processes, and the space-time yields were up to 124.5 g L^-1 d^-1. The continuous reactions were also extended to 48 h affording up to 91.8% average conversions. This study showcased the important potential to sustainable production of chiral amino alcohols in continuous flow processes.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"70"},"PeriodicalIF":4.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11258105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression, characterization, and application of human-like recombinant gelatin.","authors":"Xiaoping Song, Tao Chu, Wanru Shi, Jingyan He","doi":"10.1186/s40643-024-00785-1","DOIUrl":"10.1186/s40643-024-00785-1","url":null,"abstract":"<p><p>Gelatin is a product obtained through partial hydrolysis and thermal denaturation of collagen, belonging to natural biopeptides. With irreplaceable biological functions in the field of biomedical science and tissue engineering, it has been widely applied. The amino acid sequence of recombinant human-like gelatin was constructed through a newly designed hexamer composed of six protein monomer sequences in series, with the minimum repeating unit being the characteristic Gly-X-Y sequence found in type III human collagen α1 chain. The nucleotide sequence was subsequently inserted into the genome of Pichia pastoris to enable soluble secretion expression of recombinant gelatin. At the shake flask fermentation level, the yield of recombinant gelatin is up to 0.057 g/L, and its purity can rise up to 95% through affinity purification. It was confirmed in the molecular weight determination and amino acid analysis that the amino acid composition of the obtained recombinant gelatin is identical to that of the theoretically designed. Furthermore, scanning electron microscopy revealed that the freeze-dried recombinant gelatin hydrogel exhibited a porous structure. After culturing cells continuously within these gelatin microspheres for two days followed by fluorescence staining and observation through confocal laser scanning microscopy, it was observed that cells clustered together within the gelatin matrix, exhibiting three-dimensional growth characteristics while maintaining good viability. This research presents promising prospects for developing recombinant gelatin as a biomedical material.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"69"},"PeriodicalIF":4.3,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thermophilic and acidophilic microbial consortia on maize wet-milling steeping.","authors":"Yaqin Sun, Wenjing Xia, Langjun Tang, Zhilong Xiu, Weiwu Jin, Xiaoyan Wang, Jin Tao, Haijun Liu, Hongyan An, Yi Li, Yi Tong","doi":"10.1186/s40643-024-00783-3","DOIUrl":"10.1186/s40643-024-00783-3","url":null,"abstract":"<p><p>To understand the ecology of species and promote biotechnology through beneficial strain selection for improving starch yield in maize wet-milling steeping, bacterial diversity and community structure during the counter-current steeping process in a commercial steeping system were characterized and investigated. The microbial diversity in the steeping liquor, which consisted of 16 phyla, 131 families, and 290 genera, was more abundant compared to those present on the surface of unsteeped maize. As the counter-current steeping progressed, exposing newer maize to the older steepwater, Lactobacillus dominated, replacing Rahnella, Pseudomonas, Pantoea, and Serratia. The thermophilic and acidophilic microbial consortia were enriched through adaptive evolution engineering and employed to improve starch yield. Several steeping strategies were evaluated, including water alone, SO₂ alone, mono-culture of B. coagulans, microbial consortia, and a combination of consortium and SO₂. Combining the microbial consortium with SO₂ significantly increased the starch yield to, about 66.4 ± 0.5%, a 22% and 46% increase over SO₂ alone and the consortium alone, respectively. Scanning electron microscope (SEM) of steeped maize structure indicated that the combination of consortium and SO₂ disrupted the protein matrix and widened gaps between starch granules in maize endosperm. This released proteins into the steepwater and left starch granules in the aleurone layer. The steeping strategy of using thermophilic and acidophilic microbial consortium as additives shows potential application as an environmentally friendly alternative to conventional maize steeping procedures.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"68"},"PeriodicalIF":4.3,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into the conversion of flavin adenine dinucleotide (FAD) to 8-formyl FAD in formate oxidase: a combined experimental and in-silico study.","authors":"Kai Wen, Sirui Wang, Yixin Sun, Mengsong Wang, Yingjiu Zhang, Jingxuan Zhu, Quanshun Li","doi":"10.1186/s40643-024-00782-4","DOIUrl":"10.1186/s40643-024-00782-4","url":null,"abstract":"<p><p>Formate oxidase (FOx), which contains 8-formyl flavin adenine dinucleotide (FAD), exhibits a distinct advantage in utilizing ambient oxygen molecules for the oxidation of formic acid compared to other glucose-methanol-choline (GMC) oxidoreductase enzymes that contain only the standard FAD cofactor. The FOx-mediated conversion of FAD to 8-formyl FAD results in an approximate 10-fold increase in formate oxidase activity. However, the mechanistic details underlying the autocatalytic formation of 8-formyl FAD are still not well understood, which impedes further utilization of FOx. In this study, we employ molecular dynamics simulation, QM/MM umbrella sampling simulation, enzyme activity assay, site-directed mutagenesis, and spectroscopic analysis to elucidate the oxidation mechanism of FAD to 8-formyl FAD. Our results reveal that a catalytic water molecule, rather than any catalytic amino acids, serves as a general base to deprotonate the C8 methyl group on FAD, thus facilitating the formation of a quinone-methide tautomer intermediate. An oxygen molecule subsequently oxidizes this intermediate, resulting in a C8 methyl hydroperoxide anion that is protonated and dissociated to form OHC-RP and OH^-. During the oxidation of FAD to 8-formyl FAD, the energy barrier for the rate-limiting step is calculated to be 22.8 kcal/mol, which corresponds to the required 14-hour transformation time observed experimentally. Further, the elucidated oxidation mechanism reveals that the autocatalytic formation of 8-formyl FAD depends on the proximal arginine and serine residues, R87 and S94, respectively. Enzymatic activity assay validates that the mutation of R87 to lysine reduces the k_cat value to 75% of the wild-type, while the mutation to histidine results in a complete loss of activity. Similarly, the mutant S94I also leads to the deactivation of enzyme. This dependency arises because the nucleophilic OH^- group and the quinone-methide tautomer intermediate are stabilized through the noncovalent interaction provided by R87 and S94. These findings not only explain the mechanistic details of each reaction step but also clarify the functional role of R87 and S94 during the oxidative maturation of 8-formyl FAD, thereby providing crucial theoretical support for the development of novel flavoenzymes with enhanced redox properties.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"67"},"PeriodicalIF":4.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11236828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of earthworms and analysis of associated bacterial communities during earthworms' growth using two types of agricultural wastes.","authors":"Feng Qian, Fuzhi Lu, Liping Yang, Tingkao Li","doi":"10.1186/s40643-024-00781-5","DOIUrl":"10.1186/s40643-024-00781-5","url":null,"abstract":"<p><p>Earthworm cultivation can effectively promote the resource utilization of agricultural waste. The efficient utilization of agricultural waste by earthworms mainly depends on the microbial communities in the guts. This study used silkworm excrement and cow manure as substrates for earthworm cultivation and investigated the associated bacterial communities during earthworms' growth. The survival rate of earthworms remained above 89% after 21 days of feeding with the two substrates. Proteobacteria, Actinobacteria, and Firmicutes constituted the predominant bacterial communities in earthworm growth, accounting for over 81% of the relative abundance in both guts and vermicompost. The bacteria richness and diversity in the foregut and midgut of earthworm were lower than those in the hindgut. The prediction function of intestinal bacterial communities of earthworms cultured with two substrates mainly involved biosynthesis, decomposition and energy production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"66"},"PeriodicalIF":4.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11233487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of biochar production techniques and application in iron and steel industries.","authors":"Segun E Ibitoye, Chanchal Loha, Rasheedat M Mahamood, Tien-Chien Jen, Meraj Alam, Ishita Sarkar, Partha Das, Esther T Akinlabi","doi":"10.1186/s40643-024-00779-z","DOIUrl":"10.1186/s40643-024-00779-z","url":null,"abstract":"<p><p>Integrating innovation and environmental responsibility has become important in pursuing sustainable industrial practices in the contemporary world. These twin imperatives have stimulated research into developing methods that optimize industrial processes, enhancing efficiency and effectiveness while mitigating undesirable ecological impacts. This objective is exemplified by the emergence of biochar derived from the thermo-chemical transformation of biomass. This review examines biochar production methods and their potential applications across various aspects of the iron and steel industries (ISI). The technical, economic, and sustainable implications of integrating biochar into the ISI were explored. Slow pyrolysis and hydrothermal carbonization are the most efficient methods for higher biochar yield (25-90%). Biochar has several advantages- higher heating value (30-32 MJ/kg), more porosity (58.22%), and significantly larger surface area (113 m²/g) compared to coal and coke. However, the presence of biochar often reduces fluidity in a coal-biochar mixture. The findings highlighted that biochar production and implementation in ISI often come with higher costs, primarily due to the higher expense of substitute fuels compared to traditional fossil fuels. The economic viability and societal desirability of biochar are highly uncertain and vary significantly based on factors such as location, feedstock type, production scale, and biochar pricing, among others. Furthermore, biomass and biochar supply chain is another important factor which determines its large scale implementation. Despite these challenges, there are opportunities to reduce emissions from BF-BOF operations by utilizing biochar technologies. Overall, the present study explored integrating diverse biochar production methods into the ISI aiming to contribute to the ongoing research on sustainable manufacturing practices, underscoring their significance in shaping a more environmentally conscious future.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"65"},"PeriodicalIF":4.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11222365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regioselective and enantioselective propargylic hydroxylations catalyzed by P450tol monooxygenases.","authors":"Xu Deng, Cheng-Cheng Song, Wen-Jing Gu, Yu-Jie Wang, Lu Feng, Xiao-Jian Zhou, Ming-Qiang Zhou, Wei-Cheng Yuan, Yong-Zheng Chen","doi":"10.1186/s40643-024-00771-7","DOIUrl":"10.1186/s40643-024-00771-7","url":null,"abstract":"<p><p>Regioselective and enantioselective hydroxylation of propargylic C-H bonds are useful reactions but often lack appropriate catalysts. Here a green and efficient asymmetric hydroxylation of primary and secondary C-H bonds at propargylic positions has been established. A series of optically active propargylic alcohols were prepared with high regio- and enantioselectivity (up to 99% ee) under mild reaction conditions by using P450tol, while the C≡C bonds in the molecule remained unreacted. This protocol provides a green and practical method for constructing enantiomerically chiral propargylic alcohols. In addition, we also demonstrated that the biohydroxylation strategy was able to scaled up to 2.25 mmol scale with the production of chiral propargyl alcohol 2a at a yield of 196 mg with 96% ee, which's an important synthetic intermediate of antifungal drug Ravuconazole.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"64"},"PeriodicalIF":4.3,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Produced water treatment by semi-continuous sequential bioreactor and microalgae photobioreactor.","authors":"Nur Farahah Mohd Khairuddin, Nadeem Khan, Saravanan Sankaran, Wasif Farooq, Irshad Ahmad, Isam H Aljundi","doi":"10.1186/s40643-024-00778-0","DOIUrl":"10.1186/s40643-024-00778-0","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"63"},"PeriodicalIF":4.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant, antidiabetic, and antihyperlipidemic effects of the polyphenolic extract from Salvia blancoana subsp. mesatlantica on induced diabetes in rats.","authors":"Souad Maache, Hassan Laaroussi, Najoua Soulo, Ghizlane Nouioura, Nabil Boucetta, Mohammed Bouslamti, Hamza Saghrouchni, Yousef A Bin Jardan, Samir Ibenmoussa, Mohammed Bourhia, Badiaa Lyoussi, Ilham Elarabi","doi":"10.1186/s40643-024-00769-1","DOIUrl":"10.1186/s40643-024-00769-1","url":null,"abstract":"<p><p>Currently, several studies have demonstrated the benefits of medicinal plants in managing type 2 diabetes. In this work, we evaluated the beneficial effects of the polyphenolic extract (PESB) from Salvia blancoana subsp. mesatlantica in the management of hypercaloric-feeding and small-dose alloxan-brought type 2 diabetes in rats. We analyzed the chemical constituents of the extract, including flavones and flavonols content, to understand its biological action. The antioxidant activities were evaluated by total antioxidant action, scavenging effect of the free radical DPPH, and reducing power. The obtained results showed that the value of TFC was estimated at 31.90 ± 0.34 mgEQ/g in the PESB extract. The total antioxidant capacity was estimated at 593.51 ± 4.09 mg (EAA)/g, the value of DPPH IC₅₀ was 7.3 ± 0.00 μg/mL, and the value of EC₅₀ of reducing power was estimated at 6.43 ± 0.01 μg/mL. In total, 14 phenolic compounds were identified and the naringin was the most dominant (63.19%) while the vanillin was the less recorded (0.10%). Serum glucose decreased significantly (p < 0.05) in rats given PESB (100 mg/kg) after four weeks. Glibenclamide (GLB) and PESB reduced HbA1c and increased plasma insulin in diabetic rats, restoring HOMA-β and HOMA-IR levels to near-normal. Additionally, diabetic rats treated with GLB or PESB showed statistically equivalent results to those of non-diabetic rats regarding hepatic enzymes, renal and lipid markers, as well as cardiovascular indices. The weight loss was significantly lower in diabetic rats receiving a dose of PESB (100 mg/kg), and GLB compared to corresponding untreated diabetic rats (p < 0.01). PESB and GLB showed a prominent protective function in the pancreas, liver, and kidney tissues. This investigation demonstrates the capacity of extracts from leaves of S. blancoana subsp. mesatlantica to manage diabetes mellitus due to their richness in a wide range of bioactive compounds. Therefore, more investigations are required to estimate the safety of the plant use.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"62"},"PeriodicalIF":4.3,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}