Bioresources and Bioprocessing最新文献

{"title":"Bacillus subtilis immobilization on biochars produced from different feedstocks and pyrolysis temperatures: performance, mechanisms, and salt tolerance.","authors":"Zhixiang Jiang, Bin Liu, Rui Chen, Liankai Zhang, Guiren Chen","doi":"10.1186/s40643-026-01070-z","DOIUrl":"10.1186/s40643-026-01070-z","url":null,"abstract":"<p><p>Using biochar as a carrier to enhance the adaptability and survival of inoculated microorganisms under harsh environmental conditions is considered as a promising strategy. However, the immobilization performance and underlying mechanisms of microorganisms on different biochars remain insufficiently understood. In this study, a series of biochars were prepared from various feedstocks and at different temperatures, and their capacities to immobilize Bacillus subtilis (B.subtilis) were evaluated. The results demonstrated that high-temperature (700 °C) biochars exhibited 14.40%-60.00% greater loading capacity for B.subtilis, compared to low-temperature (400 °C) biochars. Analyses of surface morphology, functional groups, hydrophobicity, and Zeta potential before and after immobilization indicated that B.subtilis loading significantly altered the surface properties of biochar, including increases in C and N contents, enhanced richness and diversity of functional groups, and a transformation from hydrophobicity to hydrophilicity. Adsorption kinetics and isotherm modeling revealed that the immobilization process was dominated by chemical adsorption, characterized by monolayer adsorption on a homogeneous surface. Interface interaction analysis further confirmed that the electrostatic interaction, hydrogen bonding, and hydrophobic forces between the functional groups of biochar and those on B.subtilis cells synergistically facilitated microbial immobilization. Based on these findings, a two-stage adsorption process of B.subtilis on biochar was proposed: initial surface adhesion and pore filling, followed by extracellular polymer and surface functional group complexation. Biochar properties, including specific surface area, pore volume, Zeta potential, hydrophobicity, C/N ratio, and surface functional groups, were further identified as key factors influencing immobilization. Finally, the salt tolerance of B.subtilis was significantly enhanced when immobilized on biochars, particularly corn straw biochars with large specific surface areas and developed pore structures. This improved salt tolerance suggests that the biochar-based microbial fertilizers could serve as a promising approach for the amelioration of salt-alkaline soil.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13234106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148147927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CFD-aided design and hydrodynamic characterization of a single-use perfusion bioreactor for high density cell culture.","authors":"Yongqiang Liu, Qingfeng Gu, Yu Liu, Guoqian Xu, Yingping Zhuang, Meijin Guo, Chao Li","doi":"10.1186/s40643-026-01077-6","DOIUrl":"10.1186/s40643-026-01077-6","url":null,"abstract":"<p><p>To address the challenges of high-density animal cell culture, this study developed a high-density culture system comprising a single-use bioreactor (SUB) with a nominal volume of 500 mL and a pulsed tangential flow filtration (ITF) unit. The reactor can be configured with two layers of 35 mm diameter impellers-either double Elephant Ear (EE-EE) or Elephant Ear combined with Ribbon (EE-RB). The flow field characteristics were rigorously characterized through CFD simulations (60-240 rpm; 90-480 mL) validated by experimental data. Engineering analysis revealed the system's robust culture environment: [Formula: see text] values ranging from 2 to 15 h^-1 (60-180 rpm; 200-400 mL; 30-150 mL/min aeration), [Formula: see text] values from 0.1 to 10 W/m³, and mixing times between 1 and 10 s. Crucially, the system maintains a mild shear environment with an average shear strain rate (SSR) below 25 s^-1, fully within the physiological tolerance range for mammalian cells. This low-shear, high-mass-transfer design was validated through perfusion cultures of CHO and HEK293 cells. The system achieved exceptionally high cell densities of 8.32 × 10⁷ cells/mL and 1.17 × 10⁸ cells/mL, respectively, with cell viability consistently exceeding 90%, demonstrating its suitability for high-intensity biopharmaceutical production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13234075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148147946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Passiflora edulis Sims peel fermentation polysaccharide against UVB-induced photodamage via antioxidant and anti-inflammatory activities.","authors":"Jiaxuan Fang, Yifan Fang, Bingbing Fu, Zixin Song, Jianfei Zhao, Qianru Sun, Meng Li, Changtao Wang, Dongdong Wang","doi":"10.1186/s40643-026-01075-8","DOIUrl":"10.1186/s40643-026-01075-8","url":null,"abstract":"<p><p>Ultraviolet B (UVB) radiation is the primary cause of photodamage to the skin, triggering the oxidative stress, mitochondrial dysfunction, and impaired barrier integrity. We extracted polysaccharides from Passiflora edulis Sims peel via microbial fermentation (PP-FP) and characterized their structural properties using fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and molecular weight analyses. Photoprotective effects were evaluated in UVB- irradiated HaCaT cells and BALB/c mice dorsal skin by measuring of oxidative stress markers, mitochondrial function, DNA damage, and skin barrier proteins. PP-FP contains a furanose ring structure, exhibits an irregular, loosely layered morphology with attached filamentous and porous structures, and shows a number-average molecular weight (Mn) of 3.519 kDa and a weight-average molecular weight (Mw) of 6.66 kDa. In UVB-irradiated HaCaT cells, PP-FP (500 µg/mL) significantly enhanced cell migration capacity (achieving 79.39% wound closure at 48 h), reduced reactive oxygen species (ROS) accumulation, alleviated mitochondrial membrane potential aberrations, and decreased interleukin-17 (IL-17) release by 63%. Concurrently, it markedly elevated key skin barrier protein levels in HaCaT cells, notably enhancing filaggrin (FLG, 1.82-fold) and lamellar oligomerizing protein (LOR, 2.04-fold) expression. Furthermore, PP-FP maintained epidermal thickness, suppressed formation of DNA damage marker γ-H2AX, downregulated matrix metalloproteinase-3/9 (MMP3/9) expression by 40-50%, and effectively preserving epidermal barrier function. This study demonstrates that fermentation-derived Passiflora edulis Sims polysaccharides mitigate UVB damage through a dual mechanisms-antioxidant protection and barrier reinforcement-providing a sustainable strategy to repurpose agricultural byproducts for high-efficacy skincare formulations.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13234045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148147895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyurethane biodegradation by Fusarium parceramosum with insights into kinetic modelling and metabolic pathway.","authors":"Pragya Sahu, Apoorva Sherigar, Ritu Raval, Chuxia Lin, Subbalaxmi Selvaraj","doi":"10.1186/s40643-026-01079-4","DOIUrl":"10.1186/s40643-026-01079-4","url":null,"abstract":"<p><p>Polyurethane (PU) is an industrially versatile polymer whose environmental persistence pose significant waste management challenges. Although several PU-degrading microorganisms have been reported, studies evaluating degradation across chemically distinct PU substrate remain limited. The present study explores PU biodegradation potential of a soil-borne fungus Fusarium parceramosum, with two chemically different PU substrates-Impranil (PU dispersion), and PU foam-under unoptimized conditions. The fungus achieved 92% degradation of Impranil within 192 h, and 31.29% weight loss of PU foam over 60 d. Degradation kinetics were evaluated using zero-order, first-order, Langmuir, and Freundlich models, with zero-and first-order models showing best fit with experimental data. Enzymatic assay revealed significant urease and aliphatic carbamate-hydrolyzing activities associated with degradation. For Impranil, urease and aliphatic carbamate-hydrolyzing activities were 202.03 U/mL and 412.47 U/mL, respectively, whereas for PU foam they were 338.12 U/mL and 284.17 U/mL, respectively. Structural and chemical alterations were confirmed through SEM, FTIR, XRD and GC-MS analysis. Metabolite identification indicated the formation of degradation intermediates, suggesting their possible involvement in subsequent microbial processes. Overall, this study provides the first evidence of dual-substrate specificity of Fusarium parceramosum highlighting its potential in sustainable waste management approaches.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13230457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus gasseri extracellular proteins suppress acne inflammation and sebum secretion via the PI3K/AKT/mTOR pathway.","authors":"Zixin Song, Qinxuan Yuan, Yifan Fang, Bianbian Zhai, Jiman Geng, Meng Li, Changtao Wang, Dongdong Wang","doi":"10.1186/s40643-026-01074-9","DOIUrl":"10.1186/s40643-026-01074-9","url":null,"abstract":"<p><p>This study evaluated the anti-acne potential of extracellular proteins derived from Lactobacillus gasseri (LG-EPs) by integrating physicochemical characterization, antibacterial assessment, cellular assays, and an in vivo sebaceous gland model. LG-EPs were obtained by ammonium sulfate precipitation and characterized using gel permeation chromatography/light scattering (GPC/LS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The molecular weight of LG-EPs was mainly distributed between 1.0 × 10⁴ and 2.0 × 10⁴ g/mol, and peptide analysis identified four peptides with antimicrobial potential and twelve peptides with antioxidant potential. LG-EPs exhibited direct antibacterial activity against acne-associated bacteria, with MIC and MBC values of 600 µg/mL against Cutibacterium acnes and MIC and MBC values of 700 µg/mL and 1 mg/mL, respectively, against Staphylococcus aureus. Growth-curve and biofilm adhesion assays further showed that LG-EPs inhibited bacterial proliferation and adhesion. In LPS-stimulated HaCaT cells, LG-EPs reduced the secretion of pro-inflammatory cytokines, including IL-6, IL-8, IL-1β, and TNF-α, while increasing the expression of barrier-related factors such as AQP3, FLG, and LOR. In a golden hamster model, topical LG-EP treatment decreased sebum production and downregulated the transcription of lipogenesis-related genes, including SREBP-1, FAS, and ACC1. These effects were associated with reduced transcript levels of PI3K, AKT, and mTOR, while the anti-sebum effect was accompanied by increased AMPK transcription. Overall, LG-EPs showed multi-target anti-acne potential through antibacterial, anti-inflammatory, barrier-protective, and sebum-suppressive effects.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar from food processing wastes: a multi-criteria roadmap for circular energy and environmental applications.","authors":"Ahsanullah Soomro, Anıl Tevfik Koçer, Didem Balkanlı","doi":"10.1186/s40643-026-01067-8","DOIUrl":"10.1186/s40643-026-01067-8","url":null,"abstract":"<p><p>This review develops a region-specific Multi-Criteria Decision Analysis (MCDA)-based screening framework for evaluating food-processing residues for biochar applications, offering a transparent and replicable decision-support tool for policymakers and bioeconomy stakeholders in Turkey and beyond. Using Turkey as a region-specific case context, ten underutilized residues-boza fermentation residue, tarhana fines, rosehip seed cake, mulberry syrup press-cake, carob syrup pulp residue, pumpkin seed oil cake, saffron floral by-products, fig-jam seed fraction, lupin brining sediment, and date syrup filter cake-were compiled from the literature and characterized in terms of moisture, ash, organic fractions, higher heating value, and macro-mineral composition. Drawing on thermochemical fundamentals, the review synthesizes how these traits influence biochar properties relevant to fuel use, soil amendment, pollutant adsorption, anaerobic digestion (AD) enhancement, and composite materials, and qualitatively links residue groups to suitable conversion windows such as hydrothermal carbonization and low- or high-severity slow pyrolysis. To convert this information into a transparent screening tool, all indicators were normalized via min-max transformation and aggregated into four mechanistic proxies capturing fuel quality, nutrient release, an adsorption-oriented screening proxy, and AD compatibility. A Simple Additive Weighting (SAW) method was then used to calculate 0-1 suitability scores and 0-100 indices for five application domains: fuel, soil amendment, adsorption/remediation, AD enhancement, and composite/material use. Under the selected criteria and weighting assumptions, rosehip seed cake, pumpkin seed oil cake, carob syrup pulp residue, and fig-jam seed fraction emerged as comparatively high-priority feedstocks, whereas saffron floral by-products and lupin brining sediment showed consistently low relative suitability. By linking feedstock chemistry to application-oriented screening scores, the framework supports rapid comparison of residue-to-application pathways while acknowledging that rankings may evolve as additional performance data, logistical constraints, or alternative weighting scenarios are incorporated.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic activity of variously preserved extracts from spent mushroom substrate (Pleurotus ostreatus) and in vitro rumen degradation of extract-treated wheat straw.","authors":"E Daniso, P Comuzzo, M Spanghero","doi":"10.1186/s40643-026-01071-y","DOIUrl":"10.1186/s40643-026-01071-y","url":null,"abstract":"<p><p>White rot fungi obtain nutrients from fibrous substrates through the radical secretion of extracellular ligninolytic enzymes (LE) and spent mushroom substrate (SMS), a by-product of commercial cultivation, is a valuable bioresource of LE. This study aims to compare various techniques for dehydrating and preserving the Pleurotus ostreatus SMS extract in terms of enzymatic activity and ability to improve the fiber rumen degradability (NDFD) of extract-treated wheat straw. To convert the extract into stable enzyme powder, it was concentrated before being dried using freeze, spray and vacuum drying processes. The different extracts reconstituted from powders were evaluated for residual LE activity after multiple successive freeze-thaw cycles, as well as their ability to improve wheat straw NDFD. To produce extracts, fresh SMS was suspended in acetate buffer, homogenized and filtered. A portion of the original extract was stored (- 20 °C) and the remainder was concentrated up to 50% of volume by cross-flow filtration. The concentrated extract was divided into four aliquots to be used without processing or spray, vacuum or freeze drying. Extracts were evaluated for total, Mn and lignin peroxidases and laccases (LAs) enzymatic activity and were subjected to four freezing-thawing cycles. Wheat straw was incubated in reconstituted extracts before being tested for in vitro NDFD. Compared to the original extract, reductions of LE were 2-30% in concentrated and 10-36, 4-48 and 74-84% in freeze, vacuum and spray dried, respectively. LE of extracts decreased with the number of freeze-thaw cycles, apart from the spray dried extract. The NDFD of all extract-treated straws resulted higher (P < 0.05) than the control, apart from the spray-dried extract and had a high linear relationship with LAs of extracts (R² 0.87, P < 0.01). Results support the potential of extract preparations from fungal SMS to be a promising bioresource useful for improving the NDFD of high-fiber forages, such as straw.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13221525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enoki mushroom residue-based biochar loaded with Bacillus megaterium YZS-M06 improves coastal sandy soil and promotes Ipomoea pes-caprae growth.","authors":"Zongsheng Yuan, Jie Yang, Sifan Wang, Xun Dong, Xiaoling Wang, Fang Liu","doi":"10.1186/s40643-026-01073-w","DOIUrl":"10.1186/s40643-026-01073-w","url":null,"abstract":"<p><p>In this study, biochar was prepared from Flammulina velutipes residues, and then loaded with Bacillus megaterium YZS-M06, to construct a composite material (BCB treatment) with synergistic remediation function. The improvement effect of this material on coastal sandy soil and its regulatory mechanism on the growth of Ipomoea pes-caprae were investigated. The findings demonstrated that F. velutipes residue-based biochar loaded with B. megaterium YZS-M06 (BCB) exerted a pronounced synergistic effect on the amelioration of chemical properties in coastal sandy land. After 30 days of cultivation, BCB treatment significantly increased soil total nitrogen (0.65 g/kg), total potassium (12.89 g/kg), and available potassium (81.75 mg/kg), as well as urease and phosphatase activities by 221% and 210% relative to the control. It also optimized the soil bacterial community, markedly enriching Bacillus from 46.58% to 57.85% relative abundance, reshaping microbial networks, and restructuring key ecological diversity-function pathways. These combined improvements in soil physicochemical properties and microbial structure effectively promoted I. pes-caprae growth. BCB treatment enhanced root length by 101% and root number by 63%, activated leaf antioxidant systems, mitigated membrane lipid peroxidation, and boosted osmolyte accumulation, thereby systematically strengthening the plant's stress resistance. F. velutipes residue-based biochar loaded with B. megaterium YZS-M06, through the synergistic interaction of \"biochar-functional bacteria\", substantially improved soil chemical properties, optimized microbial community architecture, enhanced stress tolerance of I. pes-caprae, and promoted plant growth. This study combines the resource utilization of agricultural waste F. velutipes residues with functional microbial enhancement. Through the synergistic mechanism of biochar-functional bacteria, the dual goals of coastal sandy soil improvement and plant growth promotion were achieved, providing a new technical route and theoretical basis for ecological restoration in extreme habitats.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13212864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148026876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of microbial biocontamination in cyanobacterial ethanol synthesis via alginate encapsulation.","authors":"Xiangxiao Liu, Xiang Liu, Yanfei Pan, Huili Sun, Bo Liang, Jiahui Sun, Tianzhong Liu, Guodong Luan, Xuefeng Lu","doi":"10.1186/s40643-026-01076-7","DOIUrl":"10.1186/s40643-026-01076-7","url":null,"abstract":"<p><strong>Background: </strong>Sustainable biofuels have spurred interest in cyanobacterial ethanol production, yet large-scale application is severely hindered by microbial contamination-a devastating challenge that lacks universally effective, biocompatible mitigation strategies. Traditional methods such as pH manipulation or antibiotic application are often physiologically incompatible, environmentally unsustainable, or ineffective against diverse contaminant consortia.</p><p><strong>Results: </strong>Here, we propose and validate alginate encapsulation as a physical barrier strategy to address this challenge. Alginate, a biodegradable polysaccharide derived from brown algae, forms a porous hydrogel matrix that encapsulates cyanobacterial cells. This matrix blocks direct contact, uptake or ingestion by microbial contaminants while allowing the efficient diffusion of gases (CO₂, O₂), light, and nutrients to maintain photosynthetic and metabolic functions. Using our previously engineered, high-performance ethanol-producing strain (EP), we find that unprotected cultures rapidly collapse and cease ethanol production upon inoculation with a contaminant consortium, with cumulative ethanol yield falling to undetectable levels, whereas encapsulated cells sustain normal growth and photosynthetic activity under identical contamination pressure. After rinsing and re-cultivation, the encapsulated biomass partially restored ethanol productivity, achieving a cumulative titer of 320 mg/L over 4 days post-recovery. This suggests that the protective strategy is non-invasive and enables functional recovery of the production system following contamination exposure.</p><p><strong>Conclusions: </strong>This study demonstrates that alginate encapsulation represents a promising strategy to mitigate microbial contamination, with the potential to enhance the technical resilience and operational stability of cyanobacterial biofuel production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13212877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148026806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP regeneration-driven biocatalytic production of 1,12-dodecanediol with improved cofactor supply.","authors":"Yoon Jung Jung, Gaeul Kim, Kyungjae Yu, Byung Wook Lee, Jung Bin Shin, Jung-Oh Ahn, Si Jae Park, See-Hyoung Park, Hyun Gi Koh, Kyungmoon Park","doi":"10.1186/s40643-026-01069-6","DOIUrl":"10.1186/s40643-026-01069-6","url":null,"abstract":"<p><p>1,12-Dodecanediol is a high-value chemical widely used in the polymer and fine chemical industries. However, its industrial production is currently based on petroleum-derived processes associated with high energy consumption and environmental concerns. To address these limitations, microbial bioconversion of 1,12-dodecanedioic acid (C12 diacid) to 1,12-dodecanediol (C12 diol) has been investigated as a sustainable production route. In this pathway, carboxylic acid reductase (CAR) requires ATP and NADPH as essential cofactors, making efficient cofactor supply an important factor for achieving high conversion efficiency. In this study, a recombinant Escherichia coli-based biocatalytic system was developed to enhance C12 diol production through ATP regeneration. ATP availability was identified as an important factor for whole-cell bioconversion, and a polyphosphate kinase 2 (PPK2) from Erysipelotrichaceae bacterium was introduced to regenerate ATP from AMP, increasing the conversion yield from 22.0% to 31.8% at 40 mM substrate. Supplementation with sodium hexametaphosphate (SHMP, polyP₆) further improved diol production, reaching 19.5 mM C12 diol with a conversion yield of 48.8%. In the cell-lysate-based conversion, NADPH and NADH exhibited comparable conversion efficiencies up to 70 mM MgCl₂. Diol production was influenced by MgCl₂ concentration, with the highest conversion obtained at 100 mM MgCl₂ in the presence of NADPH, yielding approximately 28 mM C12 diol (~ 70% conversion) within 1 h. Overall, this study highlights the importance of ATP regeneration and cofactor supply for improving C12 diol production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13199544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}