Bioresources and Bioprocessing最新文献

{"title":"Efficient downstream processing of second-generation lactic acid from lignocellulosic waste using aqueous two-phase extraction.","authors":"Irene Gugel, Filippo Marchetti, Stefania Costa, Erika Baldini, Silvia Vertuani, Stefano Manfredini","doi":"10.1186/s40643-025-00847-y","DOIUrl":"10.1186/s40643-025-00847-y","url":null,"abstract":"<p><p>The utilization of low-cost, waste-derived lignocellulosic biomass for biotechnological lactic acid production is an area of significant interest. Although numerous attempts have been made to develop novel processes using second-generation feedstocks, the downstream recovery processes for second-generation lactic acid are still relatively uncharted. In this context, this study explores a two-step method for retrieving second-generation lactic acid from a fermented olive leaves medium. The initial step involves an aqueous two-phase extraction using an ethanol/ammonium sulfate system, a method first applied here to lactic acid derived from second-generation raw materials. This is followed by a purification step using activated carbon. The process succeeded in extracting second-generation lactic acid with a yield of 70.02 ± 2.29% in the first extraction stage, followed by a yield of 88.91 ± 0.49% in the second purification step. The lactic acid purity level at the end of the downstream process was 90.17 ± 1.55%, as confirmed by the HPLC data. Furthermore, the study examined the feasibility of direct lactic acid extraction from unfiltered, fermented olive leaves medium, revealing that up to 95.23 ± 0.42% of bacterial cells could be eliminated. The purified lactic acid underwent qualitative identification through FT-IR and ¹H-NMR analysis, with the spectra showing a high degree of similarity to a standard reference substance, thus demonstrating the use of low-cost waste lignocellulosic biomass for the biotechnological production of lactic acid is a topic of great interest.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"20"},"PeriodicalIF":4.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"System metabolic engineering modification of Saccharomyces cerevisiae to increase SAM production.","authors":"Liangzhuang Tan, Yuehan Zhang, Ping Liu, Yihang Wu, Zuoyu Huang, Zhongce Hu, Zhiqiang Liu, Yuanshan Wang, Yuguo Zheng","doi":"10.1186/s40643-025-00858-9","DOIUrl":"10.1186/s40643-025-00858-9","url":null,"abstract":"<p><p>S-adenosyl-L-methionine (SAM) is an important compound with significant pharmaceutical and nutraceutical applications. Currently, microbial fermentation is dominant in SAM production, which remains challenging due to its complex biosynthetic pathway and insufficient precursor availability. In this study, a multimodule engineering strategy based on CRISPR/Cas9 was established to improve the SAM productivity of Saccharomyces cerevisiae. This strategy consists of (1) improving the growth of S. cerevisiae by overexpressing the hxk2 gene; (2) enhancing the metabolic flux toward SAM synthesis by upregulating the expression of the aat1, met17, and sam2 genes and weakening the synthesis pathway of L-threonine; (3) elevating precursor ATP synthesis by introducing the vgb gene; (4) blocking the SAM degradation pathway by knocking out the sah1 and spe2 genes. The SAM titer of the resulting mutant AU18 reached 1.87 g/L, representing an increase of 227.67% compared to the parental strain. With optimal medium, SAM titer of mutant AU18 reached 2.46 g/L in flask shake fermentation. The SAM titer of mutant AU18 further reached 13.96 g/L after 96 h incubation with a continuous L-Met feeding strategy in a 5 L fermenter. Therefore, with comprehensive optimization of both synthesis and degradation pathways of SAM, a multimodule strategy was established, which significantly elevated the SAM production of S. cerevisiae. This laid a foundation for the construction of hyperproducer for SAM and other valuable amino acids or chemicals.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"19"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of olive mill solid waste by Anthracophyllum discolor and Stereum hirsutum: effect of copper and manganese supplementation.","authors":"V Benavides, A Serrano, F Pinto-Ibieta, O Rubilar, G Ciudad","doi":"10.1186/s40643-025-00842-3","DOIUrl":"10.1186/s40643-025-00842-3","url":null,"abstract":"<p><p>Anthracophyllum hirsutum and Stereum hirsutum produce manganese peroxidase (MnP) and laccase to break down lignin, a potential biological pretreatment for lignocellulosic biomass. This work aimed to evaluate the effect of copper (Cu) and manganese (Mn) added to olive mill solid waste (OMSW) inoculated with A. discolor and S. hirsutum on ligninolytic enzyme activity, lignin degradation, and phenolic compound removal. Different optimal metal dosages were determined for each fungal strain. For S. hirsutum, the addition of 6.1 mg Cu kg⁻¹ and 7.3 mg Mn kg⁻¹ resulted in 173 ± 5 U·L⁻¹ MnP activity (an 863% increase compared to no metal addition) and 42 ± 3% lignin degradation (183% higher than with no metal addition, 15 ± 3%). These conditions also led to 65-75% phenol removal efficiencies in OMSW at 25 days and 80-95% in leachates between 20 and 30 days. For A. discolor, 14.6 mg Mn kg⁻¹ yielded 37 ± 7 U·L⁻¹ MnP activity (a 142% increase compared to no metal addition) and 38 ± 7% lignin degradation (150% higher than with no metal addition, 15 ± 3%). Under these conditions, A. discolor achieved 80-90% phenol removal in leachates at 20 days. These results demonstrate the positive effect of optimised metal supplementation, highlighting the potential of S. hirsutum and A. discolor for effective lignocellulosic biomass pretreatment and future mycoremediation processes.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"18"},"PeriodicalIF":4.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in biosynthesis of chiral amide herbicides and the key enzymes: dimethenamid-P and S-metolachlor as case studies.","authors":"Feng Cheng, Yi-Ling Zhou, Dan-Chen Yang, Ding-Yi Zhao, Ya-Ping Xue, Yu-Guo Zheng","doi":"10.1186/s40643-025-00851-2","DOIUrl":"10.1186/s40643-025-00851-2","url":null,"abstract":"<p><p>Chiral amide herbicides represent a significant class of agrochemicals, widely used for effective weed control. Prominent examples include S-metolachlor and dimethenamid-P, both of which share the intermediate (S)-1-methoxy-2-propylamine, a key structural component in their synthesis. Developing green and sustainable methods for producing this intermediate is crucial for enhancing the environmental and economic feasibility of herbicide manufacturing. Biosynthesis, with its advantages in sustainability and efficiency, has emerged as a pivotal approach in pesticide production. This review explores the classification and current development status of chiral amide herbicides, including their varieties and applications in the agricultural market. It outlines the synthesis pathways for S-metolachlor and dimethenamid-P, covering both chemical and biosynthetic routes. The review also highlights the functional properties of the key enzymes involved in the biosynthesis of (S)-1-methoxy-2-propylamine, focusing on the potential for enzyme engineering and creation to optimize these pathways. The challenges and future development directions for amide herbicides are discussed, with an emphasis on overcoming synthetic and ecological barriers.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"17"},"PeriodicalIF":4.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered phenylalanine hydroxylase coupled with an effective cofactor synthesis and regeneration system for high-yield production of 5-hydroxytryptophan.","authors":"Yulin Ai, Yusong Huang, Hongru Zhao, Bingmei Su, Juan Lin","doi":"10.1186/s40643-025-00846-z","DOIUrl":"10.1186/s40643-025-00846-z","url":null,"abstract":"<p><p>5-Hydroxytryptophan (5-HTP) is widely used as a natural remedy for sleep disorders. In terms of biosafety, bio-derived 5-HTP is preferred over chemically synthesized 5-HTP. However, the low titer of 5-HTP in the reported microbiological methods (< 10 g/L) limits the industrialization of 5-HTP biosynthesis. In the present study, a Trp-accumulating E. coli strain TRP1 was constructed by blocking the degradation path (ΔtnaA), branching paths (ΔpheA, ΔtyrA) and repression system (ΔtrpR, ΔtrpL). Next, the hydroxylation module employing a phenylalanine hydroxylase mutant XcPAH^W179F (XC2) coupled with an MH4 regenerating system (CvPCD-EcFolM system) was screened to convert L-Trp into 5-HTP. Protein engineering was performed on hydroxylase XC2 based on the molecular dynamics simulation of the enzyme-substrate complex, and the strain TRP1-XC4 harboring the triple-mutant XcPAH^{L98I/A129K/W179F} (XC4) was able to produce 319.4 mg/L 5-HTP. Genome editing was carried out focused on accelerating product efflux (strengthening YddG) and increasing MH4 supply (strengthening FolM, FolE and FolX), resulting in a strain TRP5-XC4 to produce 13.9 g/L 5-HTP in 5 L fed-batch fermentation with a space-time yield of 0.29 g/L/h, which is the highest production and productivity record for 5-HTP biosynthesis. This study successfully provided an engineered strain and an efficient green method for the industrial synthesis of 5-HTP.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"15"},"PeriodicalIF":4.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties.","authors":"Pedro Barea, Alba Ester Illera, Helena Candela, Rodrigo Melgosa, José Manuel Benito, Sagrario Beltrán, María Teresa Sanz","doi":"10.1186/s40643-025-00850-3","DOIUrl":"10.1186/s40643-025-00850-3","url":null,"abstract":"<p><p>Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO₂ (subW-CO₂) and Alcalase^® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO₂ hydrolysates with polyamide membranes (800-600 Da and 300-150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase^® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1-1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"16"},"PeriodicalIF":4.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the multiple interactions between phages, microbes and flavor in the fermentation of strong-flavor Baijiu.","authors":"Huadong Zhang, Hongxia Zhang, Hai Du, Yan Zhang, Menghui Zhang, Xiaowei Yu, Yan Xu","doi":"10.1186/s40643-025-00852-1","DOIUrl":"10.1186/s40643-025-00852-1","url":null,"abstract":"<p><p>The fermentation process of strong-flavor Baijiu represents a complex and unique ecosystem, characterized by the involvement of various microorganisms that drive intricate biochemical reactions, ultimately contributing to the distinct flavor profile of the Baijiu. Viruses may affect the succession of microorganisms and thus affect the style and quality of the product. However, the interaction between viruses and microorganisms during the fermentation of Baijiu is still unclear. Here we combined viral metagenomics and amplicon sequencing, physicochemical analysis, and GC-MS detection with temporal sampling to study the dynamics of viral and microbial communities, physicochemical properties, and flavor compounds during strong-flavor Baijiu fermentation. Viral metagenomic analysis revealed 513 viral operational taxonomic units (vOTUs), encompassing 34 viral families. Principal coordinates analysis (PCoA) demonstrated significant differences in vOTUs at different fermentation stages. Notably, the microbial community exhibited distinct succession patterns at various fermentation stages; it changed rapidly during the initial five days, with similarities observed between days 10 and 20. Volatile profile analysis identified 38 flavor components in fermented grains, comprising 16 ester compounds, 11 alcohols, and 8 acids, with the majority formed between days 10 and 30. The Spearman's rank correlation analysis revealed that Peduoviridae exhibited a negative correlation with Gluconobacter. Genomoviridae showed a negative correlation with Issatchenkia, Penicillium, and Monascus. These findings highlight the potential for complex interactions between viruses and microbial communities during Baijiu fermentation, underscoring the importance of considering viral communities in studies of the microbial ecology of fermented foods.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"14"},"PeriodicalIF":4.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of free radical scavenging activity and total metabolite profiles among 30 macrofungi species.","authors":"Tetiana Krupodorova, Victor Barshteyn, Yusufjon Gafforov, Milena Rašeta, Tetiana Zaichenko, Yaroslav Blume","doi":"10.1186/s40643-025-00841-4","DOIUrl":"10.1186/s40643-025-00841-4","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The high antioxidant content of mushrooms such as polyphenols, polysaccharides, carotenoids, ergothioneine, glutathione, vitamins, and other compounds, has sparked interest in their potential use in preventive and therapeutic medicine. Therefore, this study aimed to evaluate the antioxidant potential of various macrofungi by measuring 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity and quantifying two classes of compounds: phenolics (TPC) and polysaccharides, both endo- (IPS) and exopolysaccharides (EPS). Species-specific abilities to produce mycelium, polysaccharides, and polyphenols were observed under submerged cultivation without agitation over 14 days. Mycelium productivity (P&lt;sub&gt;M&lt;/sub&gt;) ranged significantly from 292 ± 11 to 1110 ± 37 mg·L⁻&lt;sup&gt;1&lt;/sup&gt;·day⁻&lt;sup&gt;1&lt;/sup&gt;, with the highest values observed in Ophiocordyceps sinensis (1110 ± 37 mg·L⁻&lt;sup&gt;1&lt;/sup&gt; ·day⁻&lt;sup&gt;1&lt;/sup&gt;), Pleurotus djamor (1090 ± 14 mg·L⁻&lt;sup&gt;1&lt;/sup&gt;·day⁻&lt;sup&gt;1&lt;/sup&gt;), and Cordyceps militaris (1080 ± 90 mg· L⁻&lt;sup&gt;1&lt;/sup&gt;·day⁻&lt;sup&gt;1&lt;/sup&gt;). The DPPH inhibition values of the extracts varied from 4.30 ± 0.20 to 87.9 ± 0.80%, while TPC ranged from 0.35 ± 0.10 to 34.6 ± 0.80 mg GAE/g d.w. Lentinula edodes was the best source of produced IPS (10.3 ± 0.35 g·L&lt;sup&gt;-1&lt;/sup&gt;), while Hypsizygus marmoreus exhibited the highest EPS content (2.24 ± 0.30 g·L&lt;sup&gt;-1&lt;/sup&gt;). Overall, IPS levels across species were higher, ranging from 1.56 to 10.3 g·L&lt;sup&gt;-1&lt;/sup&gt;. In half of the fungal species, the mycelium exhibited superior antioxidant activity compared to culture broth. However, in the majority (66.7%), TPC was higher in the culture broth than in the fungal mycelium. Distinct scavenging abilities and metabolite profiles were observed among different isolates. Notably, the mycelium of Fomitopsis pinicola and L. edodes showed the highest DPPH inhibition and TPC. The antioxidant activity and TPC varied depending on the culture medium, and solvents used. The highest DPPH inhibition (90%) and TPC (38.5 ± 0.40 mg GAE/g d.w.) were observed in the methanol (MeOH) extract of F. pinicola mycelium grown in Sabouraud dextrose medium. Similarly, MeOH and ethyl acetate (EtOAc) extracts demonstrated strong DPPH inhibition (over 87%), while the maximum TPC (40.0 ± 0.60 mg GAE/g d.w.) was detected in the water (H&lt;sub&gt;2&lt;/sub&gt;O) extract of L. edodes mycelium cultivated in glucose-peptone-yeast medium. A positive correlation was found between TPC and antioxidant activity, with Pearson's coefficient of 0.6615 for fungal mycelium, 0.1192 for culture broth, and 0.8924 and 0.7143 for F. pinicola and L. edodes, respectively. Both fungal mycelium and culture broth exhibit significant potential as natural antioxidants. These can be applied in nutraceuticals, food products, and nutritional supplements, offering diverse health benefits. Additionally, this study is a pioneering exploration of Auriporia aurea, Hohenbuehelia myxotricha, Lepista luscina, Oxyporus obducens, and Pseudospong","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"13"},"PeriodicalIF":4.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing thermostability of Moloney murine leukemia virus reverse transcriptase through greedy combination of multiple mutant residues.","authors":"Youhui Yang, Jie Zhang, Zhong Li, Hao Qi","doi":"10.1186/s40643-025-00845-0","DOIUrl":"10.1186/s40643-025-00845-0","url":null,"abstract":"<p><p>Reverse transcription is crucial in bioengineering and biomedical fields, particularly for genome sequencing and virus diagnosis. Enhancing the thermostability of reverse transcriptase can significantly improve its efficiency and accuracy by enabling it to function at higher temperatures, thereby reducing RNA secondary structures and minimizing interference from contaminating enzymes, particularly in clinical samples. Here, using a combinatorial strategy, a variant of Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT) with improved activity across a wide temperature range (30-50 °C) was identified and maintained 100% activity after incubation at 50 °C for 10 min. Eleven hot-spot residues were mutated in various combinations, and the mutant proteins were rapidly expressed in a cell-free system for reverse transcription activity testing. Variant M5, which carries five mutated residues (E47K/E280R/T284R/L413G/D631V), exhibited enhanced thermostability and activity compared to any variant with a single residue mutation. Using purified recombinant protein for precise characterization, the melting temperature (Tm) of M5 increased by 4.7 °C when assembled with a nucleotide template-primer (T/P). Consequently, the half-life of M5 at 50 °C extended to approximately 60 min, in contrast to less than 4 min for the wild type. These findings demonstrate that the epistasis of combining multiple mutant residues holds excellent potential for significantly enhancing enzyme activity, even with existing knowledge. This heat-stable MMLV RT variant M5 may potentially improve efficiency and accuracy in molecular biology research and clinical diagnostics.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"12"},"PeriodicalIF":4.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and physicomechanical properties of cellulosic nonwovens functionalized with chitosan: a study on interaction effects of influencing factors and assessment methods.","authors":"Esubalew Kasaw Gebeyehu, Rekha Shresth, Tonmoy Saha, Jenni Tienaho, Ulla Jauhiainen, Ali Amin Tarhini, Ali Reza Tehrani-Bagha","doi":"10.1186/s40643-025-00843-2","DOIUrl":"10.1186/s40643-025-00843-2","url":null,"abstract":"<p><p>The growing demand for antimicrobial textiles and environmental concerns over synthetic agents have driven interest in biobased agents like chitosan, an eco-friendly alternative reported with variable effectiveness on textiles. This research investigates the effects of chitosan's molecular weight, concentration, treatment method, and their interaction effects on the antibacterial and physicomechanical properties of cellulosic nonwovens. The role of culture medium dynamics in antibacterial testing was also examined. Chitosan with low (30 kDa), medium (250 kDa) and high (2100 kDa) molecular weights at concentrations of 1 to 15 g/L was applied using pad-dry and dip-dry methods, with dip coating resulting in higher chitosan deposition. Antibacterial activity against Escherichia coli (E. coli) was assessed using three methods: agar diffusion, a luminescent bacterial biosensor assay, and log reduction of colony-forming units (CFU/mL) in a time-kill test. While the first two test methods showed no antibacterial effect, CFU/mL revealed significant activity with interaction effects showing that the dip-coated, 30 kDa chitosan above a concentration of 5 g/L achieved complete bacterial reduction. The enhanced antibacterial performance of chitosan-coated cellulosic substrates, compared to chitosan in a culture medium, highlighted the role of cellulosic nonwoven in boosting antibacterial effectiveness, likely through improved contact and interaction with bacterial cells. The study demonstrated that chitosan-treated cellulosic nonwovens, particularly with dip-dry coating altered tensile strength and increased the bending resistance and bending stiffness.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"11"},"PeriodicalIF":4.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}