Bioresources and Bioprocessing最新文献_第3页

Monitoring corn stover processing by the fungus Ustilago maydis 监测真菌 Ustilago maydis 对玉米秸秆的处理情况

IF 4.6 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-09-14 DOI: 10.1186/s40643-024-00802-3

Stefan Robertz, Magnus Philipp, Kerstin Schipper, Paul Richter, Katharina Miebach, Jorgen Magnus, Markus Pauly, Vicente Ramírez

{"title":"Monitoring corn stover processing by the fungus Ustilago maydis","authors":"Stefan Robertz, Magnus Philipp, Kerstin Schipper, Paul Richter, Katharina Miebach, Jorgen Magnus, Markus Pauly, Vicente Ramírez","doi":"10.1186/s40643-024-00802-3","DOIUrl":"https://doi.org/10.1186/s40643-024-00802-3","url":null,"abstract":"A key aspect of sustainable bioeconomy is the recirculation of renewable, agricultural waste streams as substrates for microbial production of high-value compounds. One approach is the bioconversion of corn stover, an abundant maize crop byproduct, using the fungal maize pathogen Ustilago maydis. U. maydis is already used as a unicellular biocatalyst in the production of several industrially-relevant compounds using plant biomass hydrolysates. In this study, we demonstrate that U. maydis can grow using untreated corn stover as its sole carbon source. We developed a small-scale bioreactor platform to investigate U. maydis processing of corn stover, combining online monitoring of fungal growth and metabolic activity profiles with biochemical analyses of the pre- and post-fermentation residues. Our results reveal that U. maydis primarily utilizes soluble sugars i.e., glucose, sucrose and fructose present in corn stover, with only limited exploitation of the abundant lignocellulosic carbohydrates. Thus, we further explored the biotechnological potential of enhancing U. maydis´ lignocellulosic utilization. Additive performance improvements of up to 120 % were achieved when using a maize mutant with increased biomass digestibility, co-fermentation with a commercial cellulolytic enzyme cocktail, and exploiting engineered fungal strains expressing diverse lignocellulose-degrading enzymes. This work represents a key step towards scaling up the production of sustainable compounds from corn stover using U. maydis and provides a tool for the detailed monitoring of the fungal processing of plant biomass substrates.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"21 2 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate). 对快速生长的纳氏弧菌进行生物合成聚（3-羟基丁酸-乳酸）工程学研究。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-09-09 DOI: 10.1186/s40643-024-00801-4

Xinye Sun, Yanzhe Shang, Binghao Zhang, Pengye Guo, Yuanchan Luo, Hui Wu

{"title":"Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate).","authors":"Xinye Sun, Yanzhe Shang, Binghao Zhang, Pengye Guo, Yuanchan Luo, Hui Wu","doi":"10.1186/s40643-024-00801-4","DOIUrl":"10.1186/s40643-024-00801-4","url":null,"abstract":"Poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] is a highly promising valuable biodegradable material with good biocompatibility and degradability. Vibrio natriegens, owing to its fast-growth, wide substrate spectrum characteristics, was selected to produce P(3HB-co-LA). Herein, the crucial role of acetyltransferase PN96-18060 for PHB synthesis in V. natriegens was identified. Heterologous pathway of P(3HB-co-LA) was introduced into V. natriegens successfully, in addition, overexpression of the dldh gene led to 1.84 fold enhancement of the lactate content in P(3HB-co-LA). Finally, the production of P(3HB-co-LA) was characterized under different carbon sources. The lactate fraction in P(3HB-co-LA) was increased to 28.3 mol% by the modification, about 1.84 times of that of the control. This is the first successful case of producing the P(3HB-co-LA) in V. natriegens. Collectively, this study showed that V. natriegens is an attractive host organism for producing P(3HB-co-LA) and has great potential to produce other co-polymers.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"86"},"PeriodicalIF":4.3,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharides and oligosaccharides originated from green algae: structure, extraction, purification, activity and applications. 源自绿藻的多糖和寡糖：结构、提取、纯化、活性和应用。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-09-06 DOI: 10.1186/s40643-024-00800-5

Chen Li, Hui Wang, Benwei Zhu, Zhong Yao, Limin Ning

{"title":"Polysaccharides and oligosaccharides originated from green algae: structure, extraction, purification, activity and applications.","authors":"Chen Li, Hui Wang, Benwei Zhu, Zhong Yao, Limin Ning","doi":"10.1186/s40643-024-00800-5","DOIUrl":"10.1186/s40643-024-00800-5","url":null,"abstract":"With the proceeding of global warming and water eutrophication, the phenomenon of green tide has garnered significant societal interest. Consequently, researchers had increasingly focused on the potential applications of green algae biomass, particularly its polysaccharides. The polysaccharide serves as the primary active constituent of green algae and has demonstrated numerous advantageous biological activities, including antioxidant, antiviral, anticoagulant, hypolipidemic and immuno-modulatory activities. The favorable bioavailability and solubility of green algae oligosaccharides are attributed to their low molecular weight. So there has been a growing interest in researching green algae polysaccharides and oligosaccharides for the utilization of marine biological resources. This review summarized the extraction, purification, chemical structure, composition, biological activity, and potential applications prospect of polysaccharides and oligosaccharides derived from green algae. The review could be helpful for expanding the applications of polysaccharides and oligosaccharides of green algae.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"85"},"PeriodicalIF":4.3,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight of effects of air quality and sustainable aviation fuel blend on energy saving and emission reduction in airport. 洞察空气质量和可持续航空燃料混合对机场节能减排的影响。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-09-04 DOI: 10.1186/s40643-024-00798-w

Ziyu Liu, Sha Yu, Xiaoyi Yang

{"title":"Insight of effects of air quality and sustainable aviation fuel blend on energy saving and emission reduction in airport.","authors":"Ziyu Liu, Sha Yu, Xiaoyi Yang","doi":"10.1186/s40643-024-00798-w","DOIUrl":"10.1186/s40643-024-00798-w","url":null,"abstract":"Air quality in airport attracts a widespread attention due to the emission of GHGs and pollutants related with aircraft flight. Sustainable aviation fuel (SAF) has confirmed PM2.5 reduction due to free of aromatics and sulphur, and thus air quality improvement in airport is prospected by SAF blend. Two types of SAF were assessed the potential of energy saving and emission reduction by ZF850 jet engine. FT fuel is characterized with only paraffins without aromatics and cycloparaffins while HCHJ fuels is characterized with no aromatics. The descend of air quality and SAF blend were both investigated the effect on the engine performance and emission characteristic. The critical parameters were extracted from fuel compositions and air pollutants. Ambient air with a higher PM2.5 could lead to the rise of engine emission especially in UHC and PM2.5 despite at the low thrust setting and high thrust setting, and even couple with 3.2% rise in energy consumption and 1% reduction in combustion efficiency. CO, NO and NO2 in ambient air show less influence on engine performance and emission characteristic than PM2.5. Both types of SAF blend were observed significant reductions in PM2.5 and UHC. PM2.5 reduction obtained 37.9%-99.8% by FT blend and 0.64%-93.9% by HCHJ blend through the whole trust settings. There are almost 6.67% positive benefit in TSFC through the whole thrust setting by 7% FT blend. The effects of air quality and SAF blend on engine emission present significant changes on PM and UHC but the slight change on CO and NOx. By SAF blend, the energy saving and pollutant reduction obtained could be both benefit for air quality improvement in airport and further reduce engine emission as the feedback of less pollutants in ambient air.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"84"},"PeriodicalIF":4.3,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax. 构建和优化基因转化系统，在亚麻中高效表达人胰岛素-GFP 融合基因。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-27 DOI: 10.1186/s40643-024-00799-9

Wei Zhao, Rui Zhang, Luyang Zhou, Zhongxia Zhang, Fei Du, Ruoyu Wu, Jing Kong, Shengjun An

{"title":"Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax.","authors":"Wei Zhao, Rui Zhang, Luyang Zhou, Zhongxia Zhang, Fei Du, Ruoyu Wu, Jing Kong, Shengjun An","doi":"10.1186/s40643-024-00799-9","DOIUrl":"10.1186/s40643-024-00799-9","url":null,"abstract":"The human insulin gene modified with a C-peptide was synthesized according to the plant-preferred codon, and a fusion gene expression vector of insulin combined with green fluorescent protein (GFP) was constructed. The optimization of the flax callus culturing was undertaken, and a more efficient Agrobacterium-mediated genetic transformation of the flax hypocotyls was achieved. The critical concentration values of hygromycin on the flax hypocotyl development, as well as on its differentiated callus, were explored by the method of antibiotic gradient addition, and the application of antibiotic screening for the verification of positive calluses was assessed. The fusion gene of insulin and GFP was successfully inserted into the flax genome and expressed, as confirmed through polymerase chain reaction and Western blotting. In conclusion, we have established a flax callus culture system suitable for insulin expression. By optimizing the conditions of the flax callus induction, transformation, screening, and verification of a transgenic callus, we have provided an effective way to obtain insulin. Moreover, the herein-employed flax callus culture system could provide a feasible, cheap, and environmentally friendly platform for producing bioactive proteins.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"83"},"PeriodicalIF":4.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on environmental factors contributing to bispecific antibody stability and the reversal of self-associated aggregates. 研究导致双特异性抗体稳定性和自结合聚集体逆转的环境因素。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-23 DOI: 10.1186/s40643-024-00796-y

Nattha Ingavat, Nuruljannah Dzulkiflie, Jia Min Liew, Xinhui Wang, Eunice Leong, Han Ping Loh, Say Kong Ng, Yuansheng Yang, Wei Zhang

{"title":"Investigation on environmental factors contributing to bispecific antibody stability and the reversal of self-associated aggregates.","authors":"Nattha Ingavat, Nuruljannah Dzulkiflie, Jia Min Liew, Xinhui Wang, Eunice Leong, Han Ping Loh, Say Kong Ng, Yuansheng Yang, Wei Zhang","doi":"10.1186/s40643-024-00796-y","DOIUrl":"10.1186/s40643-024-00796-y","url":null,"abstract":"Bispecific antibodies (bsAbs) hold promises for enhanced therapeutic potential surpassing that of their parental monoclonal antibodies. However, bsAbs pose great challenges in their manufacturing, and one of the common reasons is their susceptibility to aggregation. Building on previous studies demonstrating the functionality and potential manufacturability of Fab-scFv format bsAb, this investigation delved into the impact of environmental factors-such as pH, buffer types, ionic strength, protein concentrations, and temperatures-on its stability and the reversal of its self-associated aggregates. Mildly acidic, low-salt conditions were found optimal, ensuring bsAb stability for 30 days even at elevated temperature of 40 °C. Furthermore, these conditions facilitated the reversal of its self-associated aggregates to monomers during the initial 7-day incubation period. Our findings underscore the robustness and resilience of Fab-scFv format bsAb, further confirming its potential manufacturability despite its current absence as commercial products.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"82"},"PeriodicalIF":4.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of rare ginsenosides by biotransformation of Panax notoginseng saponins using Aspergillus fumigatus. 利用曲霉对三七皂苷进行生物转化生产稀有人参皂苷。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-09 DOI: 10.1186/s40643-024-00794-0

Lian Yang, Dongmei Lin, Feixing Li, Xiuming Cui, Dengji Lou, Xiaoyan Yang

{"title":"Production of rare ginsenosides by biotransformation of Panax notoginseng saponins using Aspergillus fumigatus.","authors":"Lian Yang, Dongmei Lin, Feixing Li, Xiuming Cui, Dengji Lou, Xiaoyan Yang","doi":"10.1186/s40643-024-00794-0","DOIUrl":"10.1186/s40643-024-00794-0","url":null,"abstract":"Panax notoginseng saponins (PNS) are the main active components of Panax notoginseng. But after oral administration, they need to be converted into rare ginsenosides by human gut microbiota and gastric juice before they can be readily absorbed into the bloodstream and exert their effects. The sources of rare ginsenosides are extremely limited in P. notoginseng and other medical plants, which hinders their application in functional foods and drugs. Therefore, the production of rare ginsenosides by the transformation of PNS using Aspergillus fumigatus was studied in this research. During 50 days at 25 ℃ and 150 rpm, A. fumigatus transformed PNS to 14 products (1-14). They were isolated by varied chromatographic methods, such as silica gel column chromatography, Rp-C18 reversed phase column chromatography, semi-preparative HPLC, Sephadex LH-20 gel column chromatography, and elucidated on the basis of their 1H-NMR, 13C-NMR and ESIMS spectroscopic data. Then, the transformed products (1-14) were isolated and identified as Rk3, Rh4, 20 (R)-Rh1, 20 (S)-Protopanaxatriol, C-K, 20 (R)-Rg3, 20 (S)-Rg3, 20 (S)-Rg2, 20 (R)-R2, Rk1, Rg5, 20 (S)-R2, 20 (R)-Rg2, and 20 (S)-I, respectively. In addition, all transformed products (1-14) were tested for their antimicrobial activity. Among them, compounds 5 (C-K) and 7 [20 (S)-Rg3] showed moderate antimicrobial activities against Staphylococcus aureus and Candida albicans with MIC values of 6.25, 1.25 μg/mL and 1.25, 25 μg/mL, respectively. This study lays the foundation for production of rare ginsenosides.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"81"},"PeriodicalIF":4.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a microbial dewaxing agent using three spore forming bacteria. 利用三种孢子形成菌研制微生物脱蜡剂。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-08 DOI: 10.1186/s40643-024-00795-z

Xiaoyan Guo, Xutao Zhao, Lizhu Li, Haibo Jin, Jianjun Wang

{"title":"Development of a microbial dewaxing agent using three spore forming bacteria.","authors":"Xiaoyan Guo, Xutao Zhao, Lizhu Li, Haibo Jin, Jianjun Wang","doi":"10.1186/s40643-024-00795-z","DOIUrl":"10.1186/s40643-024-00795-z","url":null,"abstract":"Microbial enhanced oil recovery (MEOR) is a cost effective and efficient method for recovering residual oil. However, the presence of wax (paraffin) in residual oil can substantially reduce the efficiency of MEOR. Therefore, microbial dewaxing is a critical process in MEOR. In this study, a bacterial dewaxing agent of three spore-forming bacteria was developed. Among these bacteria, Bacillus subtilis GZ6 produced the biosurfactant surfactin. Replacing the promoter of the surfactin synthase gene cluster (srfA), increased the titer of surfactin in this strain from 0.33 g/L to 2.32 g/L. The genetically modified strain produced oil spreading rings with diameters increasing from 3.5 ± 0.1 to 4.1 ± 0.2 cm. The LadA F10L/N133R mutant was created by engineering an alkane monooxygenase (LadA) using site-directed mutagenesis in the Escherichia coli host. Compared to the wild-type enzyme, the resulting mutant exhibited an 11.7-fold increase in catalytic efficiency toward the substrate octadecane. When the mutant (pIMPpladA2mu) was expressed in Geobacillus stearothermophilus GZ178 cells, it exhibited a 2.0-fold increase in octadecane-degrading activity. Cultures of the two modified strains (B. subtilis GZ6 (pg3srfA) and G. stearothermophilus GZ178 (pIMPpladA2mu)) were mixed with the culture of Geobacillus thermodenitrificans GZ156 at a ratio of 5:80:15. The resulting composition increased the rate of wax removal by 35% compared to the composition composed of three native strains. This study successfully developed a multi-strain bacterial agent with enhanced oil wax removal capabilities by genetically engineering two bacterial strains.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"80"},"PeriodicalIF":4.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation: the best solution to the world problem of discarded polymers. 生物降解：解决废弃聚合物这一世界性难题的最佳方案。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-07 DOI: 10.1186/s40643-024-00793-1

Jun Wu, Jia Wang, Yicheng Zeng, Xinxiao Sun, Qipeng Yuan, Ling Liu, Xiaolin Shen

{"title":"Biodegradation: the best solution to the world problem of discarded polymers.","authors":"Jun Wu, Jia Wang, Yicheng Zeng, Xinxiao Sun, Qipeng Yuan, Ling Liu, Xiaolin Shen","doi":"10.1186/s40643-024-00793-1","DOIUrl":"10.1186/s40643-024-00793-1","url":null,"abstract":"The widespread use of polymers has made our lives increasingly convenient by offering a more convenient and dependable material. However, the challenge of efficiently decomposing these materials has resulted in a surge of polymer waste, posing environment and health risk. Currently, landfill and incineration treatment approaches have notable shortcomings, prompting a shift towards more eco-friendly and sustainable biodegradation approaches. Biodegradation primarily relies on microorganisms, with research focusing on both solitary bacterial strain and multi-strain communities for polymer biodegradation. Furthermore, directed evolution and rational design of enzyme have significantly contributed to the polymer biodegradation process. However, previous reviews often undervaluing the role of multi-strain communities. In this review, we assess the current state of these three significant fields of research, provide practical solutions to issues with polymer biodegradation, and outline potential future directions for the subject. Ultimately, biodegradation, whether facilitated by single bacteria, multi-strain communities, or engineered enzymes, now represents the most effective method for managing waste polymers.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"79"},"PeriodicalIF":4.3,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis. 移除血球藻的鞭毛，通过能量重新分配提高虾青素的积累。

IF 4.3 3区生物学

Bioresources and Bioprocessing Pub Date : 2024-08-02 DOI: 10.1186/s40643-024-00789-x

Yuyong Hou, Zhile Guo, Zhiyong Liu, Suihao Yan, Meijie Cui, Fangjian Chen, Weijie Wang, Longjiang Yu, Lei Zhao

{"title":"Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis.","authors":"Yuyong Hou, Zhile Guo, Zhiyong Liu, Suihao Yan, Meijie Cui, Fangjian Chen, Weijie Wang, Longjiang Yu, Lei Zhao","doi":"10.1186/s40643-024-00789-x","DOIUrl":"10.1186/s40643-024-00789-x","url":null,"abstract":"Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"11 1","pages":"78"},"PeriodicalIF":4.3,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0