Abdellah Baraich, Amine Elbouzidi, Mohamed Jeddi, Mohamed Taibi, Mounir Haddou, Naoufal El Hachlafi, Reda Bellaouchi, Ramzi A Mothana, Mohammed F Hawwal, Mohamed Addi, Larbi Rhazi, Redouane Benabbes, Abdeslam Asehraou, Bassem Jaouadi, Ennouamane Saalaoui
{"title":"Optimized formulation of a three-component extract mixture from Moroccan Crocus sativus L. (Stigmas, leaves, and Tepals) for enhanced antioxidant activity.","authors":"Abdellah Baraich, Amine Elbouzidi, Mohamed Jeddi, Mohamed Taibi, Mounir Haddou, Naoufal El Hachlafi, Reda Bellaouchi, Ramzi A Mothana, Mohammed F Hawwal, Mohamed Addi, Larbi Rhazi, Redouane Benabbes, Abdeslam Asehraou, Bassem Jaouadi, Ennouamane Saalaoui","doi":"10.1186/s40643-025-00892-7","DOIUrl":"10.1186/s40643-025-00892-7","url":null,"abstract":"<p><p>This study evaluated the antioxidant potential of a three-component mixture derived from Crocus sativus L. stigmas, leaves, and tepals, aiming to valorize these underutilized by-products. Hydroethanolic extracts were chemically profiled using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD), identifying major bioactive compounds including crocin (11.04%), rutin (22.32%), and ellagic acid (62.47%). Antioxidant capacity was assessed using DPPH and ABTS assays, yielding IC<sub>50</sub> values ranging from 88.67 ± 0.83 µg/mL to 291.41 ± 0.91 µg/mL for DPPH, and from 116.76 ± 1.31 µg/mL to 286.87 ± 0.25 µg/mL for ABTS. A simplex-centroid mixture design was employed to determine optimal component ratios. The best-performing DPPH mixture (43% stigmas, 35% leaves, 22% tepals) achieved an IC<sub>50</sub> of 97.36 ± 1.23 µg/mL, closely matching the predicted 95.96 µg/mL. Similarly, the optimal ABTS blend (45% stigmas, 35% leaves, 20% tepals) reached an IC<sub>50</sub> of 110.59 ± 6.82 µg/mL, aligning with the predicted 106.31 µg/mL. Both combinations outperformed standard antioxidants like BHT and ascorbic acid. These findings support the synergistic use of saffron by-products in food preservation, nutraceuticals, and biopharmaceuticals, offering an eco-friendly, cost-effective solution aligned with circular economy principles.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"94"},"PeriodicalIF":5.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhancement of Menaquinone-7 production in Bacillus subtilis by optimizing the medium components through response surface methodology.","authors":"Rui Zhang, Han Wang, Li Wang, Zhiming Zheng","doi":"10.1186/s40643-025-00934-0","DOIUrl":"10.1186/s40643-025-00934-0","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"93"},"PeriodicalIF":5.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utilizing network pharmacology and other tools to examine active components and mechanism of action of Magnolia officinalis rheum rhabarbarum decoction in treating Streptococcus pyogenes skin infections.","authors":"Yuanhao Wang, Xinrui Wang, Xueying Zhang, Mengyi Pan, Mingyang Sun, Zhiguo Chen, Yingli Song","doi":"10.1186/s40643-025-00933-1","DOIUrl":"10.1186/s40643-025-00933-1","url":null,"abstract":"<p><p>Infections caused by Streptococcus pyogenes and the growing threat of antibiotic resistance pose significant global health challenges. This study investigates the antibacterial properties of Magnolia officinalis Rheum rhabarbarum Decoction against Streptococcus pyogenes skin infections. By combining UHPLC-MS/MS, network pharmacology, and molecular docking techniques, we identified eight bioactive compounds in the formulation and explored their potential interactions with Streptococcus pyogenes-related targets. Our analysis revealed that compounds such as Sinensetin, Nobiletin, and (+)-Magnoflorine regulate immune pathways (IL-17, TNF), inhibit the production of inflammatory factors, and disrupt bacterial membranes and metabolic processes, achieving dual antibacterial and anti-inflammatory effects. In vitro experiments showed that the decoction exhibited a minimum inhibitory concentration (MIC) of 20 mg/mL against Streptococcus pyogenes, significantly reducing the secretion of pro-inflammatory factors such as IL-1α, IL-6, IL-36, and TNF-α. These results suggest that Magnolia officinalis Rheum rhabarbarum Decoction offers a promising multi-target strategy for treating drug-resistant Streptococcus pyogenes infections and may serve as a potential alternative to traditional antibiotics.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"92"},"PeriodicalIF":5.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khushboo Iqbal, Arti Mishra, Smitha Mony Sreedharan
{"title":"The quintessence of algal biomass in bioplastic production: insightful advancement and sustainable use.","authors":"Khushboo Iqbal, Arti Mishra, Smitha Mony Sreedharan","doi":"10.1186/s40643-025-00908-2","DOIUrl":"10.1186/s40643-025-00908-2","url":null,"abstract":"<p><p>Plastics are essential components of modern life, and their global demand is increasing daily. They are gaining recognition as a sustainable source for bioplastic production due to their rapid growth, carbon fixation ability, and capacity to utilize various waste streams. It seems that landfill, incineration, chemical treatment, and plastic recycling are not the best options for minimizing plastic pollution. A novel approach A new approach is needed to reduce this pollution. Bioplastics are biodegradable and come with less toxicity, a low carbon footprint, and are a better alternative to fossil-based plastics. This review explores recent advances in algal bioplastics, focusing on key polymers like polyhydroxyalkanoates (PHAs) and polylactic acid (PLA). Special attention is given to the use of genetic tools such as CRISPR-Cas systems to improve yield and carbon flux. Challenges related to downstream processing, low biomass productivity, and environmental variability are also discussed. This review highlights the importance of standardized life cycle assessments (LCAs) to evaluate environmental impact across the entire production chain. Additionally, regulatory frameworks from different countries are compared to identify gaps and promote progressive policy development. The review aims to provide an integrated perspective on the technical innovation, economic feasibility, and policy needed to support the future of algae-based bioplastics.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"91"},"PeriodicalIF":5.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of ultrasound pre-treatments before enzymatic hydrolysis on physicochemical, nutritional and functional properties of insoluble protein fractions obtained from Atlantic mackerel side streams.","authors":"Janna Cropotova, Kristine Kvangarsnes, Elissavet Kotsoni, Revilija Mozuraityte, Inger Beate Standal, Amélie Le Gall, Turid Rustad","doi":"10.1186/s40643-025-00931-3","DOIUrl":"10.1186/s40643-025-00931-3","url":null,"abstract":"<p><p>This study investigated the impact of ultrasound (US) pre-treatment before enzymatic hydrolysis on physicochemical, nutritional, and functional properties of insoluble protein fractions obtained from fish side streams after filleting of Atlantic mackerel (heads, backbones, fins, tails and trimmings). Four fractions were analyzed: a control without US-treatment and US-treated samples at 300 W, 450 W, and 600 W. The results showed that ultrasonication significantly increased total protein content in insoluble protein fraction after the treatment at 300 W. Additionally, salt-soluble proteins increased significantly in 450 W and 600 W samples, while water-soluble proteins increased after US-treatment at 600 W, suggesting that higher US-intensities promoted protein bond breakdown and exposure of hydrophilic amino acid groups. The degree of hydrolysis increased significantly in 450 W sample due to cavitation effect of ultrasound. Total thiols decreased in water-soluble proteins after US-treatment at 300 W and 450 W, as well as in all salt-soluble proteins compared to control due to unfolding of proteins and breakage of disulfide bonds. No significant differences were detected in lipid oxidation markers (TBARS and ¹H NMR aldehyde profile). From the nutritional perspective, the nutritional indexes of lipid quality AI (atherogenicity index), TI (thrombogenicity index), and HH (hypocholesterolemic fatty acid ratio) were favorable for all insoluble protein fractions. US-treatment enhanced the hypocholesterolemic profile of insoluble protein fractions by lowering their HH index. P-NMR lipid profiling identified PC and PC-ether as the predominant phospholipids (70-74% of total PL content). No significant differences were found in color parameters (L, a, b*) between control and US-treated samples at 300 W and 450 W.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"90"},"PeriodicalIF":5.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sustainability through lignin valorization: recent innovations and applications driving industrial transformation.","authors":"Poulose Sarojam Jiju, Anil Kumar Patel, Nalinakshan Sreevidya Shruthy, Saseendran Shalu, Cheng-Di Dong, Reeta Rani Singhania","doi":"10.1186/s40643-025-00929-x","DOIUrl":"10.1186/s40643-025-00929-x","url":null,"abstract":"<p><p>Lignin, the second most abundant natural polymer, is emerging as a strategic feedstock for advancing a sustainable and circular bioeconomy. Traditionally underutilized byproduct of lignocellulosic biomass processing, lignin is now central to the development of bio-based chemicals, advanced polymers, and renewable fuels. This review explores recent progress in lignin valorization, focusing on emerging strategies in chemical, thermophysical, and biological depolymerization. Innovations in catalytic conversion, ionic liquid-assisted processing, and biocatalytic approaches are enabling the selective transformation of lignin into high-value products, offering viable alternatives to petroleum-derived inputs. Emerging lignin-based technologies are being adopted across key industries such as packaging, textiles, automotive, construction, and energy, enabling the development of eco-friendly and high-value product solutions. Despite its significant potential, lignin valorization is constrained by challenges including structural complexity, inefficient processing, and scalability limitations. Addressing these issues requires integrated strategies that combine technological innovation, interdisciplinary collaboration, industrial integration, and supportive policy frameworks. Through a critical assessment of the opportunities and limitations in lignin valorization and technology development, this study establishes lignin as a strategic component of next-generation biorefinery systems, advancing green material innovation and enabling resource-efficient, low-emission production in alignment with global sustainability objectives and future industrial research.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"88"},"PeriodicalIF":5.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thai Anh Do, Van Quyen Nguyen, Thi Minh Chau Nguyen, Thi Hang Nga Nguyen, Thi Huong Le
{"title":"A one-step chemical treatment to directly isolate microcrystalline cellulose from lignocellulose source.","authors":"Thai Anh Do, Van Quyen Nguyen, Thi Minh Chau Nguyen, Thi Hang Nga Nguyen, Thi Huong Le","doi":"10.1186/s40643-025-00920-6","DOIUrl":"10.1186/s40643-025-00920-6","url":null,"abstract":"<p><p>In the study, we report an effective and one-step chemical treatment for directly isolating microcrystalline cellulose (MCC) with a width of 10-50 μm and length of 100-1000 μm from various agricultural waste sources. This chemical treatment uses a peracetic acid solution combined with 2% H₂SO₄ at 80 °C for 2 h. After this one -step chemical treatment, we observed significant changes in chemical composition. For example, the cellulose percentage substantially increased across all biomass sources: from 26.73 to 55.94% for dragon fruit foliage, and from 41.26 to 64.13% for corn cob, and from 17.86 to 67.38% for banana pseudostem. Simultaneously, the lignin content decreased from 22.05 to 1.03%, 12.74 to 3.76% and 14.27 to 2.73%, respectively for dragon fruit foliage, corn cob, and banana pseudostem. XRD confirmed that the crystallinity of the bleached cellulose is high (above 65%), and elemental analysis showed the existence of only O and C elements in the bleached sample. We attributed the effectivity of our chemical treatment to the combination effect of solvent polarity, strong oxidative agent and H<sub>2</sub>SO<sub>4</sub> acid, which allows the removal of extractive, the lignin and partially hydrolysis hemicellulose component simultaneously at the short time. Our microcrystalline cellulose can serve as an effective matrix for gel polymer electrolytes, exhibiting good mechanical behavior and excellent electrical properties, with highest ionic conductivity of 197 mS. cm<sup>-1</sup>. Our study opens a new, simple path to directly isolate microcrystalline cellulose from lignocellulosic source and extend the application of microcrystalline cellulose toward the field in energy storage and conversion.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"89"},"PeriodicalIF":5.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engineering a modular pectin-to-lipids bioconversion system using two Kluyveromyces marxianus strains.","authors":"Bofan Yu, He Qiao, Xuye Lang","doi":"10.1186/s40643-025-00927-z","DOIUrl":"10.1186/s40643-025-00927-z","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"87"},"PeriodicalIF":5.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jameelah Alhadi Salih Othman, Norzita Ngadi, R A Ilyas, Abu Hassan Nordin, M F M Alkbir, Mohd Nor Faiz Norrrahim, Victor Feizal Knight, Khalid A Alzahrani, Anish Khan, Pijush K Mondal
{"title":"Development and characterization of magnetic polyethyleneimine-bamboo nanocellulose adsorbents for enhanced performance of dye pollutant removal.","authors":"Jameelah Alhadi Salih Othman, Norzita Ngadi, R A Ilyas, Abu Hassan Nordin, M F M Alkbir, Mohd Nor Faiz Norrrahim, Victor Feizal Knight, Khalid A Alzahrani, Anish Khan, Pijush K Mondal","doi":"10.1186/s40643-025-00928-y","DOIUrl":"10.1186/s40643-025-00928-y","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"86"},"PeriodicalIF":5.1,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytoextract-mediated Cupper nanoparticles via Acacia saligna: synthesis, characterization and in vitro anticancer and apoptosis inducing effects.","authors":"Fuad O Abdullah","doi":"10.1186/s40643-025-00918-0","DOIUrl":"10.1186/s40643-025-00918-0","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"85"},"PeriodicalIF":5.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}