Bioresources and Bioprocessing最新文献

{"title":"Bacillus subtilis spore surface display enhances manganese peroxidase stability and stress resistance.","authors":"Lu He, Mati Ullah, Muhammad Naeem, Zhong Ni, Yong Feng, Tawaf Ali Shah, Molalign Assefa, Khalid S Almaary, Huayou Chen","doi":"10.1186/s40643-025-00901-9","DOIUrl":"10.1186/s40643-025-00901-9","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"57"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Escherichia coli-driven whole-cell bioconversion for selective 5-Aminopentanol production as a novel bioplastic monomer.","authors":"Byung Wook Lee, Hee Taek Kim, Hyun Gi Koh, Kyungjae Yu, Gaeul Kim, Yoon Jung Jung, Haeng-Geun Cha, Yunhee Jeong, Yung-Hun Yang, See-Hyoung Park, Kyungmoon Park","doi":"10.1186/s40643-025-00904-6","DOIUrl":"10.1186/s40643-025-00904-6","url":null,"abstract":"<p><p>5-Aminopentanol (5-AP) is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics. Conventional production methods rely on petroleum-based feedstocks and metal catalysts, which raise environmental and sustainability concerns. In this study, a de novo biosynthetic pathway for 5-AP production from L-lysine was developed in Escherichia coli. The engineered pathway consisted of lysine decarboxylase 2 (LdcC), putrescine aminotransferase (PatA), and tested aldehyde reductase (YahK, YihU, YqhD). Among the tested reductases, aldehyde reductase exhibited the highest catalytic efficiency, producing 44.5 ± 2.6 mM of 5-AP (0.44 ± 0.03 mol_5 - AP/mol_l-lysine). The replacement of the expression system with a T7-based dual-plasmid platform, pET24ma::ldcC, and pCDFDuet-1::yqhD::patA co-transformed into E. coli, increased the production to 60.7 ± 5.8 mM, accompanied by reduced cadaverine accumulation. Further enhancement was achieved by increasing the gene dosage of PatA, leading to 68.5 ± 4.2 mM 5-AP and reduced by 40% in cadaverine levels. Cadaverine is a precursor in the production of 5-AP, and its accumulation is an important factor in the limitation of conversion to 5-AP. Intracellular cofactor regeneration is expected to cause an indirect supply of α-KG, a cofactor, to enhance conversion to 5-AP. To support intracellular cofactor regeneration, glucose supplementation and increased aeration were applied, resulting in a final titer of 78.5 ± 1.2 mM 5-AP and improved precursor utilization. This study is the first report of selective microbial 5-AP production and highlights the importance of PatA expression in pathway optimization. The newly established L-lysine (C6) valorization process which converts L-lysine to high-value materials such as 1,5-PDO, glutarate, and 5-AP offers a promising route for the sustainable biosynthesis of amino alcohols, laying the groundwork for future improvements through enzyme engineering and metabolic design.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"58"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting bacterial colonization and biofilm formation for enhanced biodegradation of low-density polyethylene microplastics.","authors":"Marwa Gamal Eldeen Afify, Ola M Gomaa, Hussein Abd El Kareem, Mohamed A Abou Zeid","doi":"10.1186/s40643-025-00902-8","DOIUrl":"10.1186/s40643-025-00902-8","url":null,"abstract":"<p><p>The accumulation of plastic waste presents a significant worldwide environmental challenge. This study aimed to isolate polyethylene-degrading bacteria from marine samples containing plastic waste. Four culturable bacterial isolates: Micrococcus luteus, Bacillus cereus, Enterococcus faecalis, and Actinomyces sp. were assessed for their biofilm formation, biosurfactant, and protease production. Gamma irradiation was used to induce structural changes and promote bacterial colonization and biofilm formation on low-density polyethylene microplastics (LDPE MPs). Optimal biofilm formation was achieved in minimal media supplemented with 30% tryptic soy broth, 10% biosurfactant, and 300 µM calcium chloride. The factorial design experiment demonstrated that adding media supplementation significantly improved bacterial colonization and biofilm formation when compared to gamma irradiation. This was supported with Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) mapping, and Fourier Transform Infrared Spectroscopy (FTIR). The optimized LDPE MP degradation was achieved through a multi-step protocol: (1) samples are pre-treated to 40 kGy gamma irradiation, which resulted in 5.7% Gravimetric weight loss and structural and morphological changes, (2) incubation in biofilm inducing media overnight, and (3) further incubation in minimal media for 30 days. This approach resulted in a total weight loss of 22.5%. In conclusion, synergistic pre-treatment is recommended to promote biofilm and improve biodegradation of LDPE MPs by marine bacteria.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"59"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive preservative nano-packaging films based on food wastes of orange peels and Shrimp for apple (Malus domestica var. Anna) fruit quality and storage.","authors":"Mohamed S Hasanin, Mahmoud Emam, M A Ahmed, F M Rohim, M A A Mohamed, Housni El Saied, Hamdy A Z Hussein, A Abdelkhalek","doi":"10.1186/s40643-025-00890-9","DOIUrl":"10.1186/s40643-025-00890-9","url":null,"abstract":"<p><p>Storage of economic fruits is a fatal economic and nutritional factor for most countries. Edible coating played a restricted role in this manner, forcing good usability with many limitations. In this work, Apple (Malus domestica var. Anna) was coated using bioactive nanopackaging films formulated to overcome the economic limitations and drawbacks of conventional coating. The formulated bioactive nanopackaging was based on nanochitosan prepared from shrimp shells and orange peel waste, which is used to produce nanocellulose (white part) and extract active ingredients (orange part). The formulated bioactive nanopackaging based on nanochitosan and nanocellulose (2:1) and orange peel waste extract with ratios of 1, 3, and 5% (w/w) based on nanochitosan dry weight and called T3, T4, and T5, respectively. Characteristics of bioactive nanopackaging films and their precursor materials were characterized physicochemically and topographically as well. The waste orange peel waste extract was characterized phytochemically. According to the orange peel extract, the formulated bioactive nanopackaging films observed antioxidant and antimicrobial activity. The results revealed that all treatments outperformed the control, especially treatments T4 (1% Nano Chitosan + 1% Nano Cellulose + 3% Orange Peel Waste extract) and T5 (1% Nano Chitosan + 1% Nano Cellulose + 5% Orange Peel Waste extract), in terms of fruit decay percentage (11.72 ± 11.4 C and 12.33 ± 10.83 C, respectively), weight loss (3.81 ± 2.29B and 3.77 ± 2.22B, respectively), TSS/acidity (17.07 ± 1.14 A and 16.77 ± 1.18 A, respectively), fruit firmness (12.83 ± 1.19B and 13.48 ± 0.91 A, respectively), total sugars (7.98 ± 0.21 A and 8.21 ± 0.29 A, respectively) and total anthocyanin (0.21 ± 0.03B and 0.25 ± 0.05 A, respectively).</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"54"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling anti-atherosclerosis mechanism of anthocyanins from Xinjiang wild cherry plum (Prunus divaricata Ledeb) via network pharmacology and molecular docking.","authors":"Siyu Li, Juan He, Huiyi Hu, Guang Wang, Juan Tang, Jun Yao, Jing Shen, Xing Li","doi":"10.1186/s40643-025-00900-w","DOIUrl":"10.1186/s40643-025-00900-w","url":null,"abstract":"<p><p>Atherosclerosis is a chronic vascular disease characterized by failure to resolve inflammation and forming plaque within the arterial wall. Atherosclerosis and its related cardiovascular diseases are the major causes of death worldwide. Our previous preliminary study showed that anthocyanin-rich extract (ACNE) from Xinjiang wild cherry plum (Prunus divaricata Ledeb) fruit peels exhibited anti-atherosclerotic effect. However, the potential mechanism of this health-beneficial effect remains unclear. Here, network pharmacology combined with molecular docking was used to tentatively address this issue. The ACNE mainly contains cyanidin, cyanidin 3-glucoside (Cy3Glu), Cyanidin 3-(6''-acetylglucoside) (Cy3AcGlu), cyanidin 3-galactoside (Cy3Gal), cyanidin 3-xyloside (Cy3Xyl), and cyanidin 3-rutinoside (Cy3Rut). Seven key targets, EGFR, VEGFA, HSP90AA1, SRC, HIF1A, CXCR4 and IGF1R were identified from core protein-protein interaction (PPI) network. Anthocyanins interacting on key targets were initially demonstrated by molecular docking, particularly Cy3Rut and Cy3Xyl having highest affinity with most key targets. Biological function analysis suggested that key targets were involved in several biological processes, including positive regulation of cell migration, positive regulation of phosphorylation, inflammatory response, response to hypoxia, etc. The significantly enriched pathways, such as HIF-1 signaling pathway, calcium signaling pathway, macrophage stimulating protein MSP signaling network map, were closely related to atherosclerosis. Altogether, based on the comprehensive analysis and discussion, we revealed that TLR4/EGFR and IGF1R-CXCL12/CXCR4 pathways were at least partially implicated in the anti-atherosclerotic effects of anthocyanins through affecting inflammation, endothelial homeostasis, and foam cell formation. This study served as a theoretical basis for further validating the underlying anti-atherosclerotic mechanism of anthocyanins via in vitro and in vivo experiments.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"53"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of heterogeneous catalysts in the preparation of bio-based platform compound 5-hydroxymethylfurfural.","authors":"Yuyuan He, Jinrong Zhou, Han Li, Lin Deng, Jinnan Gao","doi":"10.1186/s40643-025-00894-5","DOIUrl":"10.1186/s40643-025-00894-5","url":null,"abstract":"<p><p>In recent years, there has been growing attention towards developing renewable energy and materials derived from abundant biomass resources. 5-Hydroxymethylfurfural (HMF) is recognized as a promising bio-based platform compound for synthesizing value-added chemicals and materials due to its versatile reactivity. HMF can be directly synthesized from carbohydrates and various raw biomass through the acid-hydrolysis reaction. Heterogeneous catalysts have gained prominence in biomass conversion owing to their environmental friendliness, facile separation from reaction mixtures, high catalytic efficiency, and reduced corrosivity toward equipment. This review systematically examines the reaction pathways and mechanisms involved in HMF synthesis from fructose, glucose, cellulose, and raw biomass using heterogeneous catalysts. Then we give an introduction to the preparation of furandicarboxylic acid (FDCA) from HMF with different catalytic methods. FDCA is an important degradable bio-material monomer for polyethylene furanoate to replace petroleum-based polyethylene terephthalate. This review ends with a prospect on the challenges and opportunities of HMF synthesis in the near future.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"55"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation into the role of layered nanoporous modified clays for adsorption/desorption of rosemary essential oil in the gas phase and the ability of this system as an antioxidant and preservation agent in food applications.","authors":"Doha Berraaouan, Mohamed Tirinsi, Ramzi A Mothana, Hanan M Al-Yousef, Marie-Laure Fauconnier, Samira Salhi, Abdesselam Tahani","doi":"10.1186/s40643-025-00884-7","DOIUrl":"10.1186/s40643-025-00884-7","url":null,"abstract":"<p><p>A new green composite material with antioxidant properties and specific-targeting capabilities was developed using Montmorillonite Clay in modified forms for rosemary essential oil gaseous adsorption without harmful chemicals or excessive heat. The modified clays were tested for their adsorption capabilities, with the sodic clay showing the highest affinity for the essential oil. The study also found that the adsorbed essential oil on sodic clay had the highest antioxidant activity compared to the organic and pillared clays. Adsorption isotherms revealed values of 40, 30, and 18 mg g^-1 for sodic, organic, and pillared clay respectively. To assess the experimental data three models were used: First Order, Second Order, and Intra-particular Diffusion. According to the models' data, the Second Order model fits the experimental results. These results suggest combining modified clays with essential oils can create materials suitable for controlled release systems in food and pharmaceutical applications. Highlights The capacity of gaseous adsorption of REO on sodic, organic, and pillared clays depends essentially on the interaction between the clay's external surface and the essential oil-active molecules. Adsorbed essential oil on clays' surfaces without any direct contact shows an antioxidant activity against DPPH that can be explored in further research concerning food packaging, drug delivery…etc. The desorbed amount of REO is practically less than half of the adsorbed quantity which involves both physisorption and chemisorption interactions.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"56"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of calcium oxide nanocatalyst and application in transesterification of waste cooking oil.","authors":"Rajni Garg, Rishav Garg, Nnabuk Okon Eddy, Mukhtar Iderawumi Abdulraheem, Oluwadamilola Oluwatoyin Hazzan, Gholaremza Abdi","doi":"10.1186/s40643-025-00879-4","DOIUrl":"10.1186/s40643-025-00879-4","url":null,"abstract":"<p><p>This study aims to synthesize calcium oxide nanoparticles by employing green synthetic methods and explore their potential as nano-catalyst based upon the utilization of waste into a value-based product. Waste orange peel extract has been utilized as a reducing medium. The reaction was optimized by varying the reactants' molar ratio to obtain calcium carbonate microparticles that were calcined to obtain calcium oxide nanoparticles with a particle size ranging from 70 to 100 nm. Various spectrochemical techniques analyzed the composition and morphology of the nano-catalyst. The nano-catalyst was further exploited in the one-pot transesterification of waste cooking oil. The biodiesel was analyzed for the presence of methyl ester groups by FTIR and GCMS analysis. The impact of varying reaction constraints, including temperature, contact time, nano-catalyst concentration, and methanol-oil molar ratio, were critically analyzed to optimize biodiesel yield. The study provided an economical and environmentally benign technique to successfully synthesize calcium oxide nano-catalyst to obtain biodiesel with 93.4% yield and effective waste minimization.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"52"},"PeriodicalIF":4.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on techno-economic assessment of Spirulina for sustainable nutraceutical, medicinal, environmental, and bioenergy applications.","authors":"Musa Nasiru Musa, Ghazali Musa Jirgi, Zakariyya Uba Zango, Marnawi Nasiru Isah, Muhammad Abdurrazak, Adamu Ahmad Adamu, Ismael A Wadi, Adekunle Akanni Adeleke, Zaharaddeen N Garba, Usman Bello, Haruna Adamu, Ahmad Hosseini-Bandegharaei, Dmitry Olegovich Bokov","doi":"10.1186/s40643-025-00888-3","DOIUrl":"10.1186/s40643-025-00888-3","url":null,"abstract":"<p><p>Global population growth underlies the need to explore alternative materials to address pressing challenges in food security, medicine, energy, and environmental pollution. Spirulina is a nutrient dense cyanobacteria that offers promising solutions to the aforementioned challenges, mainly due to its rich composition of proteins, vitamins, minerals, and bioactive compounds such as β-carotene and phycocyanin. These compounds confer various health benefits, including antioxidant, anticancer, anti-diabetic, antimicrobial, and anti-inflammatory properties, which make Spirulina a valuable dietary and therapeutic supplement. Essential fatty acids and its rapid growth rate also makes Spirulina a potential source of biodiesel for energy related applications. Additionally, Spirulina's high porosity and variable functional groups endow it with remarkable biosorption properties for soil and wastewater remediation applications. The chemical structure and unique properties of Spirulina have been utilized to produce biotemplates for nanomaterials as well as the fabrication of functional composites for various applications. Thus, in this review, we have highlighted the broad potentials of Spirulina in diverse applications, emphasizing its eco-friendliness, economic viability, challenges, and the prospects of its biomass for sustainable, nutraceutical, therapeutic, energy related, and environmental applications.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"51"},"PeriodicalIF":4.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of agri-food crucifer vegetables waste for food, functional food and nutraceuticals applications.","authors":"Lereen Khaled, Nada M Ali, Reem Nader, Ninon Rolet, Elizabeth S Sadek, Mohamed A Farag, Tamer Shoeib","doi":"10.1186/s40643-025-00895-4","DOIUrl":"10.1186/s40643-025-00895-4","url":null,"abstract":"<p><p>Valorization of food byproducts, especially fruits and vegetables, has recently attracted considerable attention, mostly due to their high wastage rates. Exploitation of these byproducts, including the non-edible parts of crucifer vegetables, may provide value-added opportunities in the food, functional food, and nutraceutical industries as well as in non-food applications such as therapeutics, biofuels, and paper pulp production. This review focuses on the state-of-the-art valorization practices of crucifer vegetable agro-food wastes including those of broccoli, cauliflower, cabbage, kale, Brussels sprouts, collards, watercress and radish constituting the main cultivated crucifer vegetables worldwide and suggests potential novel uses through upcycling. A detailed comparative phytochemical composition of crucifer vegetable waste products as potential sources of raw materials in promising applications including the production of food enhancers, and antioxidants is presented. Different extraction techniques combining downstream and white biotechnology processes for the optimum utilization of such agro-food waste are discussed. The valorization of cruciferous vegetables by-products is shown to be economical, sustainable and a viable approach to unlock novel applications across diverse industries. To fully maximize the potential of these underutilized resources and promote an ecological bioeconomy, more research and development into extraction methods and upcycling techniques is needed.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"50"},"PeriodicalIF":4.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}