Bioresources and Bioprocessing最新文献

{"title":"Cultivation strategy optimization and pilot-scale production of Spirulina subsalsa grown in seawater and monosodium glutamate wastewater.","authors":"Mingyan Liu, Liqun Jiang, Ze Yu, Meng Ma, Huiying Chen, Haiyan Pei","doi":"10.1186/s40643-025-00926-0","DOIUrl":"10.1186/s40643-025-00926-0","url":null,"abstract":"<p><p>Spirulina subsalsa represents a promising candidate for commercial production. Yet, due to its unique pattern of attached growth followed by upward flotation, there is an urgent need to find a suitable cultivation strategy and achieve scale-up. In the low-cost medium of seawater plus monosodium glutamate wastewater, this study initially investigated the influence of aeration rate (0, 0.3, 0.6 L/min) and reactor geometry (D₂₄d_4.5, D₁₈d₈, D₁₂d_18, D: diameter, cm; d: depth, cm) on microalgal growth, indicating that Spirulina subsalsa was suited for culturing in non-aerated, wide-shallow reactors. Then, in the single plastic reactor, diverse depths (2.25, 4.50, 9.00 cm) and surface areas (1000, 2000, and 3000 cm²) for culturing Spirulina subsalsa were optimised to 4.5 cm and 2000 cm². Subsequently, a pilot-scale cultivation system with a volume of 162 L was constructed, adopting a semi-continuous culture mode. The average dry mass productivity was 0.12 g/L/d and 816.48 g of algal powder was harvested within 41 days. The mean contents of protein, carbohydrate and lipid were respectively 46.50%, 14.95% and 10.87%. Moreover, economic analysis demonstrated that the cost of produced algal powder was 8.30 USD/kg. In the future, Spirulina subsalsa has the potential to be developed into multiple products.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"83"},"PeriodicalIF":5.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil.","authors":"Elmin Rahic, Nicholas Cassady, Kurt Rosentrater","doi":"10.1186/s40643-025-00924-2","DOIUrl":"10.1186/s40643-025-00924-2","url":null,"abstract":"<p><p>This study explored a novel laccase production process utilizing perennial biomass and the aqueous phase from bio-oil. Experimental and techno-economic modeling were coupled to gain insight into and optimize the solid-state fermentation conditions for minimizing the laccase selling price. At relatively small scales, processing only 230 Mg of prairie biomass per year, a 5-year return on investment required a minimum laccase selling price of $0.05/kU, assuming a 10% discount rate. The associated sensitivity analysis showed the minimum laccase selling price to be most sensitive to parameters affecting laccase output from the plant, which included the number of batches produced per year and laccase recovery assumptions. Limitations and future outlooks were provided, emphasizing the need for further process optimization to minimize fermentation time and downstream losses.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"84"},"PeriodicalIF":5.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the common mechanisms and therapeutic targets of medicinal herbs in acute pancreatitis: a network pharmacology and experimental validation approach.","authors":"Yuxin Shi, Yan Jia, Ya Liu, Hanyue Wang, Honghui Liu, Yilin Huang, Peiyan Chen, Jie Peng","doi":"10.1186/s40643-025-00925-1","DOIUrl":"10.1186/s40643-025-00925-1","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"82"},"PeriodicalIF":5.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable lactic acid production from agricultural waste: a review of current techniques, challenges and future directions.","authors":"Nurasyikin Abdul Rahman, Zainab Ngaini, Saba Farooq, Sabrina Chua Ai Ling, Puteri Nabilah Jefree Shahren","doi":"10.1186/s40643-025-00923-3","DOIUrl":"10.1186/s40643-025-00923-3","url":null,"abstract":"<p><p>The increasing demand for lactic acid (LA), driven by its applications in biodegradable plastics, food additives, pharmaceuticals, and cosmetics, necessitates the development of sustainable production methods. Traditional processes often rely on food-based feedstock, aggravating food security concerns. This review addresses the critical gap in utilising agricultural waste as a non-food feedstock for LA production, thereby reducing waste and promoting a circular economy. Current techniques are thoroughly explored, including fed-batch, batch, and continuous fermentation procedures, as well as the utilisation of various microbial strains, including Lactobacillus species. Recent developments in genetic engineering and innovative fermentation techniques are discussed, alongside challenges such as substrate variability and contamination. By highlighting the potential of agricultural residues, such as sugarcane bagasse, rice husk and corn stover, this review provides a roadmap for future research and industrial applications to enhance sustainability and efficiency in LA production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"81"},"PeriodicalIF":5.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of aroma profile and microbial community of cigar tobacco leaves from different aging periods and varieties and their correlations analysis.","authors":"Jian Liu, Zelin He, Qing Lin, Can Lyu, Junwei Zhao, Xiang Li, Xueying Wang, Yansong Xiao, Yang Ning","doi":"10.1186/s40643-025-00906-4","DOIUrl":"10.1186/s40643-025-00906-4","url":null,"abstract":"<p><p>In this study, the CTLs from Hainan, China, were used, the aroma profiles of CTLs from five years (2017-2021) and three varieties [Hainan2 (HN2), Jianheng3 (JH3), and Guyin4 (GY4)] were analyzed, and the microbial communities of these CTLs and the relation with aroma constituents were explored. Results revealed the contents of total aroma constituents (3.55-7.67 mg/kg), esters (47.4-824 μg/kg), alcohols (123-561 μg/kg), aldehydes (51.4-97.1 μg/kg), and nitrogen heterocycles (1.86-4.25 mg/kg), firstly increased and subsequently declined with the increase of years, whereas ketones (1.07-3.56 mg/kg) exhibited an inverse trend. Among the different varieties, the highest aldehyde (142 μg/kg) content was observed in JH3, while the highest total aroma constituents, acids, ketones, and nitrogen heterocycles were found in GY4, reaching 10.5, 0.604, 1.86, 6.43 mg/kg, respectively. Furthermore, the bacterial communities of CTLs from different years exhibited discernible succession with increasing years. The abundances of Staphylococcus (3.03-47.5%) and Sphingomonas (1.18-10.2%) first increased and then decreased, whereas Pseudomonas (1.63-11.5%), Ralstonia (0.669-20.2%), and Pantoea (6.07-10.5%) exhibited the opposite trend, and Aspergillus and Cladosporium with total abundances of 90.2-94.2% were predominant in all years. The dominant bacteria and fungi of CTLs from different varieties were identified as Staphylococcus (15.0-81.1%) and Aspergillus (45.6-85.0%), respectively. However, the dominant microbial abundances in JH3 were significantly lower than those in HN2 and GY4, while other microorganism abundances were increased. Partial least square-regression analysis demonstrated that Paracoccus, Staphylococcus, Aerococcus, Pantoea, Methylobacterium-Methylorubrum, and Bacillus of CTLs from different years were associated with aroma constituents.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"80"},"PeriodicalIF":5.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12304363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The driving role of extracellular polymeric substances in the bioelectrical conversion of petroleum hydrocarbons by rhizosphere microbial fuel cells: bioelectricity production, substrate bioconversion, microbial function and their network correlation.","authors":"Lanmei Zhao, Yuru Wen, Lyu Can, Long Meng, Jian Liu, Dong Zhao","doi":"10.1186/s40643-025-00922-4","DOIUrl":"10.1186/s40643-025-00922-4","url":null,"abstract":"<p><p>Extracellular polymeric substances (EPS), are crucial components of biofilms that drive the bioelectrical conversion of petroleum hydrocarbons (PHCs), but their role has not been adequately addressed. This research explores the driving role of EPS in bioelectrical PHC conversion by rhizosphere microbial fuel cells (MFCs). We found that current density, output voltage, coulombic efficiency, power density, current stabilization time, metabolite volatile fatty acid (VFA) production and PHC biodegradation ratio initially increased and then decreased with rising initial EPS level (0-128 mg·g^- 1), peaking at 64 ± 1 mA·m^- 2, 8.04 ± 0.16 V, 60.9 ± 1.2%, 129 ± 3 mW·m^- 2, 23 ± 1 days, 1.77 ± 0.04 g·kg^- 1 and 66.7 ± 1.5%, respectively. Fluorescence intensity of proteins having tyrosine-tryptophan demonstrated a continuous enhancement, consistent with increased biofilm thickness. Within an appropriate range of initial EPS levels (0-64 mg·g⁻¹), bioelectricity generation and PHC bioconversion enhanced as the EPS content rose in mature biofilms. However, excessive EPS addition could increase biofilm thickness to 0.48 mm, which in turn reduced biofilm activity and overall system performance. The abundances of electrochemically active and PHC-degrading bacteria presented an initial increase followed by a subsequent decrease as the initial EPS level rose, highlighting that EPS at the optimal level enriched and activated these functional bacteria. The positive correlations between the relative abundances of these bacteria and various metrics of bioelectricity generation and PHC bioconversion underscored the critical role of EPS in shaping microbial community structure and enhancing electron transfer efficiency through biofilm formation and stabilization. These findings not only provide a critical theoretical foundation and novel ideas to promote the conversion of PHC into renewable bioenergy but also highlight the potential scalability and environmental benefits of this technology in the field of clean remediation of PHC-polluted soils and recovery of bioenergy. Integrating EPS-driven MFCs with other renewable energy technologies will offer promising opportunities to develop hybrid systems that generate clean energy while mitigating environmental pollution. Furthermore, this approach also has the potential as biosensors for the real-time detection of PHCs, thus contributing to broadening its application in environmental monitoring.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"79"},"PeriodicalIF":5.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar microbiota confers deeper color of fermented cigar wrapper under additional fermented bacteria.","authors":"Qian Wu, Xuanshuang Duan, Dapeng Tang, Rui Wang, Fuqiang Li, Jun Tan, Honghua Chen, Shixue Zheng","doi":"10.1186/s40643-025-00921-5","DOIUrl":"10.1186/s40643-025-00921-5","url":null,"abstract":"<p><p>Microorganisms play an indispensable role in the fermentation of cigar tobacco such as improvement of flavor, aroma and quality. However, little is known about additional microorganism impact on shift of the color of cigar wrappers. Here, three bacterial strains that could alter the wrapper's color were isolated and screened from cigar tobacco leaves (CTLs) at different stages of fermentation. The three strains were identified to be Sphingomonas parapaucimobilis DS1, Kosakonia cowanii DS101, and Comamonas thiooxydans DS309 based on the genome sequences. The overall color difference (∆E) of CTLs was significantly deepened with the addition of those three strains under upscaled stack fermentation, respectively (p ≤ 0.05), showing the same fundamental quality of cigar wrapper. The ascorbic acid oxidase activity was significantly decreased and cigarillo polyphenol oxidase activity was significantly increased added with the three bacteria compared to the control (p ≤ 0.05). Moreover, the exogenous strains substantially altered the community structure of the cigar wrapper. In particular, the abundance of dominant Staphylococcus nepalensis significantly increased under addition of strain DS101 and DS309 except for strain DS1 as dominant species. In addition, non-targeted metabolomics analyses showed that the exogenous microbial addition treatments demonstrated substantial variations in a variety of metabolites, including a reduced expression in the pathways related to the manufacture of polyphenols and unsaturated fatty acids (p ≤ 0.05). In conclusion, addition of bacteria DS1, DS101, and DS309 enhanced oxidation of polyphenols to brown quinones and oxidation of unsaturated fatty acids, resulting in membrane lipid peroxidation and then deepened the color of cigar wrappers. It provides a novel strategy to improve the color of cigar wrappers by utilizing additional bacteria in cigar fermentation.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"78"},"PeriodicalIF":5.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using of transporter proteins to improve the uptake efficiency of hydrophobic compounds by Escherichia coli: a coordinated synthesis of START protein and P450scc system proteins to enhance cholesterol biotransformation.","authors":"Sofia V Zamalutdinova, Ludmila V Isaeva, Yaroslav V Faletrov, Nikolay N Eroshchenko, Alexey N Kirushin, Vadim N Tashlitsky, Mikhail A Rubtsov, Ludmila A Novikova","doi":"10.1186/s40643-025-00909-1","DOIUrl":"10.1186/s40643-025-00909-1","url":null,"abstract":"<p><p>Synthesis of steroid drugs is possible through biotechnological methods, however, the low efficiency of transporting steroid substrates across microbial membranes is the challenge in using microorganisms for their production. STARD1 and STARD3 proteins (members of the START domain family) work in some mammalian cells in conjunction with the steroidogenic P450scc enzyme system (cytochrome P450scc, adrenodoxin reductase, and adrenodoxin), transporting cholesterol to the mitochondria, where cytochrome P450scc converts it into pregnenolone. In this study, we investigated the effect of STARD1 and STARD3 on P450scc efficiency in E. coli expression models. The combination of the STARD3(216-444) or STARD1(53-285) protein bearing an N-terminal periplasm-targeting sequence, with the P450scc system reconstituted in E. coli, resulted in an increase of pregnenolone synthesis by 3.2- and 1.9-fold, respectively, on a laboratory scale. STARD3(216-444) showed higher levels of expression and a greater effect on the incorporation of sterols into cells and whole-cell biotransformation of cholesterol, compared to STARD1(53-285). This study proposes a fundamentally new approach to optimizing the functioning of the P450scc system in microbial cells, which uses the cholesterol transfer protein to increase the uptake efficiency of a poorly soluble steroid substrate by bacteria. The demand for steroid medications is increasing, and the use of specific carrier proteins could be a useful tool to enhance the efficiency of whole-cell biosynthesis of various steroid compounds.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"77"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Optimization of extraction process and solvent polarities to enhance the recovery of phytochemical compounds, nutritional content, and biofunctional properties of Mentha longifolia L. extracts.","authors":"Meryem Tourabi, Khaoula Faiz, Rachid Ezzouggari, Bouchra Louasté, Mohammed Merzouki, Musaab Dauelbait, Mohammed Bourhia, Khalid S Almaary, Farhan Siddique, Badiaa Lyoussi, Elhoussine Derwich","doi":"10.1186/s40643-025-00910-8","DOIUrl":"10.1186/s40643-025-00910-8","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"76"},"PeriodicalIF":4.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12274164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive expression of eukaryotic cytochrome P450 ferulate-5-hydroxylase in Escherichia coli for sustainable synthesis of antioxidant 5-hydroxyferulic acid.","authors":"Ping Sun, Yuan Tian, Luyi Wang, Pengcheng Chen, Dan Wu, Pu Zheng","doi":"10.1186/s40643-025-00919-z","DOIUrl":"10.1186/s40643-025-00919-z","url":null,"abstract":"<p><p>The eukaryotic cytochrome P450 ferulate-5-hydroxylase (F5H), a membrane-bound protein, plays a critical role in lignin synthesis involved in the biosynthesis of 5-Hydroxyferulic acid (5-HFA) from ferulic acid, with 5-HFA offering enhanced antioxidant properties. However, there is challenging to engineer recombinants for 5-HFA synthesis by expressing F5H in Escherichia coli. In this work, we co-expressed F5H derived from Arabidopsis thaliana (AtF5H) and NADPH-dependent cytochrome P450 reductase (CPR) in E. coli, and successfully synthesized ortho-hydroxylated ferulic acid. Simultaneously, by modifying the N-terminal regions of membrane proteins, we increased the concentration of product 5-HFA to 63.6 mg/L. Ferulic acid, utilized as the substrate, was extracted from discarded agricultural by-products, demonstrating a sustainable approach to valorizing agricultural waste. This work also advances the application of plant-derived membrane-bound proteins for the production of plant secondary metabolites in E. coli.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"75"},"PeriodicalIF":4.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}