Bioresources and Bioprocessing最新文献

{"title":"Nitrogen deprivation differentially alters lipid accumulation and pyrolysis-derived volatile profiles in three microalgal species.","authors":"Carlos Vicente Garza-León, Hans Christian Correa-Aguado, Gloria Viviana Cerrillo-Rojas, Roberto Rico Martínez, Omar Gómez-García, Iván Salgado-Transito","doi":"10.1186/s40643-026-01114-4","DOIUrl":"https://doi.org/10.1186/s40643-026-01114-4","url":null,"abstract":"<p><p>Nitrogen deprivation (N-) is widely used to induce lipid accumulation in microalgae. However, its impact on the relationship between lipid accumulation and thermochemical behavior remains poorly understood. In this study, a comparative experimental approach was used to evaluate the effects of nitrogen availability on growth kinetics, lipid accumulation, and pyrolysis-derived volatile compounds in Chlorella vulgaris, Scenedesmus obliquus, and Nannochloropsis oculata. Under nitrogen-replete conditions (N +), all species showed higher growth rates, whereas N- reduced proliferation but increased lipid content by 5.0-fold in C. vulgaris, 2.4-fold in S. obliquus, and 1.8-fold in N. oculata. Py-GC/MS analysis revealed that N- shifted the pyrolysis profile toward lipid-derived compounds, particularly C16-C18 fatty acids, long-chain alcohols, and hydrocarbons. In C. vulgaris, oleic acid (22.3%) and alcohol derivatives dominated; in contrast, N + conditions exhibited a more heterogeneous profile, including aromatics and sterols. N. oculata maintained a predominance of lipid-derived compounds, with N- associated with a higher contribution of oxygenated compounds. In S. obliquus, the pyrolysis profile under N + was characterized primarily by esters and reactive intermediates, while under N- it was dominated by hydrocarbons (13.6%) and alcohols. Nitrogen deprivation increased lipid accumulation in all three microalgal species, but the volatile compounds generated during pyrolysis remained species-dependent. Despite the higher lipid content under nitrogen deprivation, the corresponding pyrolysis profiles did not show a consistent relationship with lipid accumulation. These results indicate that lipid-rich biomass does not necessarily exhibit similar thermochemical behavior across microalgal species and suggest that evaluating feedstocks based solely on total lipid content may overlook species-specific differences relevant to bioenergy production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and spoilage potential of yeasts isolated from traditional Egyptian Karish cheese.","authors":"Reda H Abdel-Fadeel, Sherif A Abdella, Tarek M Abdelghany","doi":"10.1186/s40643-026-01116-2","DOIUrl":"https://doi.org/10.1186/s40643-026-01116-2","url":null,"abstract":"<p><p>Traditional soft cheeses are susceptible to microbial contamination, particularly when unsterilized flavoring additives are used during production or retail handling. This study aimed to quantify and molecularly characterize the yeast flora associated with spiced Karish cheese, a traditional Egyptian fresh cheese supplemented with peppers and olives. Yeast counts ranged from 2.25 to 2.99 log₁₀ CFU/g, while molds were detected in 60% of the samples, reaching 1.47 log CFU/g. These counts exceeded the maximum permissible limits specified by the Egyptian Standard (ES 1008-4/2005), indicating potential deficiencies in hygienic practices during production, handling, or storage. Morphological and physiological characterization grouped the 12 yeast isolates into four phenotypic profiles. Representative non-Saccharomyces isolates were further identified by sequencing the ITS1-5.8 S-ITS2 rDNA region, followed by BLASTN analysis and phylogenetic reconstruction using the Neighbor-Joining method with 1,000 bootstrap replicates. Two representative species were identified as Kodamaea ohmeri (AZ EG 3) and Pichia kudriavzevii (AZ EG 2). BLASTN analysis showed > 99% sequence identity with representative GenBank sequences, while phylogenetic analysis placed both isolates within well-supported monophyletic clades (bootstrap > 95%), with evolutionary distances of 0.009 and 0.020 substitutions/site, respectively. The representative sequences were deposited in GenBank under accession numbers PX406129 and PX503693. These findings provide baseline molecular data for the surveillance of spoilage yeasts in traditional Egyptian dairy products. Although the role of spice additives was not directly investigated, the results support the need for future studies to evaluate their contribution as potential sources of yeast contamination in artisanal cheese production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of fermentation parameters for the valorization of tomato pomace into biomass to produce single cell protein using Saccharomyces cerevisiae.","authors":"Ayse Sultan Akgun, Deniz Sevim Cabuk, Dila Cinar, Omer Alp, Dilara Kaya, Nigar Guliyeva, Beste Naz Ince, Defne Gulcek, Mecit Halil Oztop","doi":"10.1186/s40643-026-01115-3","DOIUrl":"https://doi.org/10.1186/s40643-026-01115-3","url":null,"abstract":"<p><p>Tomato pomace, a byproduct of tomato processing industry, can be utilized through fermentation to produce single cell protein (SCP). In this study, fermentation parameters were optimized in a 3-L bioreactor using a full factorial design (FFD), to which a second order polynomial regression model was subsequently fitted to locate the optimum via response surface analysis. While pH, temperature, agitation speed and airflow rate were held constant, the effects of solid load, inoculum volume, and fermentation period were analyzed using a 3 × 3 × 5 experimental design. The resulting biomass was harvested, and its protein content was analyzed. Optimal conditions were identified as a 10.35% solid load and a 2.07% inoculum volume over a 5-day fermentation period. Under these optimized conditions, the model-predicted dry cell weight of 6.92 g/L was validated experimentally at 6.25 g/L, with protein content of 38.40%. These results demonstrate the effective valorization of tomato industry waste into a high value protein resource through optimized bioprocessing.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic pathway engineering of Escherichia coli for enhanced 3'-sialyllactose production.","authors":"Yan Wu, Taoling Min, Zhenfeng Ma, Shu Wang, Chuanmin Sun, Hanzhi Zhang, Chenxi Huang, Xudong Qu, Zhenhua Tian","doi":"10.1186/s40643-026-01110-8","DOIUrl":"10.1186/s40643-026-01110-8","url":null,"abstract":"<p><p>3'-Sialyllactose (3'-SL), the simplest sialylated human milk oligosaccharide (HMO), is widely incorporated into infant formula due to its roles in shaping the gut microbiota, inhibiting pathogen adhesion, and supporting neurodevelopment. In this study, we developed an efficient microbial cell factory for 3'-SL production by enhancing carbon utilization and CTP regeneration. Following the identification of a highly active α-2,3-sialyltransferase, metabolic flux was redirected through pfkA deletion and fbaA overexpression. The precursor pool was strengthened by optimizing the expression of key enzymes involved in UDP-GlcNAc and CMP-Neu5Ac biosynthesis. To further improve pathway balance and stability, promoter engineering was applied to optimize the expression of four key genes (neuA, neuB, neuC and ST). The optimized multigene cassette was subsequently integrated into the chromosome using a one-step multicopy integration strategy, generating a plasmid-free and antibiotic marker-free production strain. The resulting strain achieved 3'-SL titers of 7.75 g L^-1 in shake flasks and 102.18 g L^-1 in a 5-L fed-batch bioreactor, representing, to the best of our knowledge, the highest reported level to date. This work demonstrates a robust strategy combining metabolic rewiring, adaptive gene expression tuning and multicopy genome integration, providing a versatile platform for the sustainable production of 3'-SL and other high-value HMOs.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesembryanthemum crystallinum -guided green synthesis of ZnO nanoparticles with selective anticancer activity via ROS-mediated mitochondrial dysfunction.","authors":"Sara Taha, Nahed M M Emam, Salem S Salem, Mohamed Nagui T Attia, Metwally M Montaser","doi":"10.1186/s40643-026-01107-3","DOIUrl":"10.1186/s40643-026-01107-3","url":null,"abstract":"<p><p>Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Mesembryanthemum crystallinum L. (M. crystallinum), a halophytic plant adapted to saline environments of North Sinai, Egypt, offers a sustainable nanofabrication strategy with inherent therapeutic potential. High-resolution LC-ESI-QTOF-MS metabolomic profiling identified 13 major phytochemicals in the ethanolic leaf extract, dominated by citramalate (peak area 31,601), L-phenylalanine (30,377), and stress-responsive organic acids, reflecting the plant's halophytic adaptation. These redox-active compounds templated the biosynthesis of crystalline ZnO-NPs under ambient aqueous conditions, yielding spherical to quasi-spherical nanoparticles (4.4-12.2 nm primary size; 40-150 nm aggregates) with a hexagonal wurtzite structure, as confirmed by XRD and SAED. FTIR and EDX analyses verified surface functionalization by phytochemical capping agents (polyphenols, organic acids), while SEM revealed characteristic aggregation with rough surface morphology indicative of biomolecular adsorption. Biosynthesized ZnO-NPs exhibited selective cytotoxicity against human malignant melanoma A375 cells (IC₅₀ = 100.55 ± 8.6 µg/mL) with four-fold lower toxicity toward normal human skin fibroblasts (IC₅₀ = 406.01 ± 35.2 µg/mL; selectivity index ≈ 4.0). Mechanistic investigations demonstrated that nanoparticle internalization triggered robust ROS generation (~ 3.8 × 10⁴ fluorescence units), mitochondrial membrane depolarization, and mixed apoptotic/necrotic cell death (40% total death; 25% late apoptosis, 15% necrosis). Cell cycle analysis revealed cancer-selective G2/M arrest in A375 cells (20.87% vs. 8.59% control; p < 0.001) versus protective G1 arrest in fibroblasts (69.14% vs. 56.01% control; p < 0.001). Paradoxically, qPCR showed BAX downregulation in cancer cells but upregulation in normal cells, suggesting non-transcriptional execution of apoptosis in malignancies. These findings establish M. crystallinum as an ecologically sustainable nanofactory whose stress-adapted metabolome directs the formation of selectively cytotoxic ZnO-NPs acting through ROS-mediated mitochondrial dysfunction, a paradigm for designing plant-guided nanotherapeutics with defined molecular mechanisms.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of biosurfactant production by Rhodococcus ruber IEGM 231 grown on waste cooking oil, their emulsifying activity and antioxidant potential.","authors":"Maria S Kuyukina, Anastasiia V Krivoruchko, Irina B Ivshina","doi":"10.1186/s40643-026-01102-8","DOIUrl":"10.1186/s40643-026-01102-8","url":null,"abstract":"<p><p>In recent years, the production of microbial biosurfactants, increasingly used in various industries and agriculture, from low-cost renewable substrates is intensively studied. Large volumes of food waste, in particular waste cooking oil, are promising substrates for the production of biosurfactants on an industrial scale. However, studies on the ability of Rhodococcus bacteria, well-known biosurfactant producers, to grow on this substrate and the functional characteristics of synthesized biosurfactants are still scarce. In this study, the possibility of improving biosurfactant production by Rhodococcus ruber IEGM 231 grown on waste cooking oil was investigated using multifactor analysis and response surface methodology (RSM). Using standardized parameters for the concentrations of carbon and nitrogen sources in the fermentation medium, a Pareto diagram was constructed, which shows a direct dependence of the crude biosurfactant yield on the oil concentration, while the concentrations of sugar and inorganic nitrogen salts were found insignificant. Using RSM, the optimal ratio of the medium parameters was determined for the maximum yield of crude biosurfactants: oil (+ 1), sugar (0), NH₄- and NO₃-containing salts (- 1) in the following concentrations: 5.0 vol%, 2.5 g/L, 0.1 and 0.2 g/L. Crude biosurfactants (40 g/L) obtained from excess waste oil under nitrogen limitation contained three glycolipid fractions, including predominant monoacyl trehalose, previously identified for this strain grown on n-hexadecane, and a considerable amount of residual non-converted acylglycerides. The synthesized biosurfactants produced stable emulsions from petroleum products, food and cosmetic oils and demonstrated high antioxidant capacity in the in vitro ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging test. Overexpression of the fbpB gene encoding mycolyltransferase, a key enzyme of the final stage of trehalolipid assembly was revealed in the cells grown on waste cooking oil, which indicated the induction of the synthesis of glycolipid biosurfactants. These findings open up the possibility of valorization of large-scale food oil waste into value-added biosurfactants using Rhodococcus.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating multi-omics with regular analyses to elucidate the effects of ZnO nanomaterials on improving soybean quality.","authors":"Xinqi Hu, Yi Zhao, Shixin Cai, Yuting Zhang, Xiaoli Wang, Nandi Zhou","doi":"10.1186/s40643-026-01109-1","DOIUrl":"10.1186/s40643-026-01109-1","url":null,"abstract":"<p><p>With rising global demand for high-quality food crops, soybean production is constrained by an inherent yield-quality trade-off that conventional practices cannot easily overcome. Zinc oxide nanomaterials (ZnO NMs) show great potential for crop improvement, but their molecular mechanism underlying soybean quality regulation remains unclear. This study investigated the effects of ZnO quantum dots (ZnO QDs, 5.56 nm) and ZnO nanoparticles (ZnO NPs, 29.68 nm) on soybean growth and quality via root and foliar application with different concentrations (0, 5, 10, 20, 50 and 100 mg/kg). Among these experimental groups, root application of 50 mg/kg ZnO QDs (R-QD-50) was selected as the optimal combination based on regular analyses and used for integrated transcriptomic, proteomic and metabolomic analyses. The results showed that ZnO NMs significantly promoted soybean growth, yield and quality in ZnO NMs type, application mode and concentration-dependent manner. Multi-omics integration identified three common pathways: taurine and hypotaurine metabolism, butanoate metabolism, and phenylpropanoid biosynthesis. Among them, only phenylpropanoid biosynthesis formed a complete transcriptome-proteome-metabolome regulatory chain. Key genes phenylalanine ammonia lyase (PAL) and cinnamic acid-4-hydroxylase (C4H) were significantly upregulated, the related synthases increased by ~ 35% at the protein level, and downstream metabolites including isoflavones, lignin and phenolic acids were increased by ~ 52%. Overall, this study clarified the potential molecular mechanism of ZnO NMs in improving soybean quality and provided theoretical reference for the application of ZnO NMs in soybean quality improvement under controlled pot conditions.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13442772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive laboratory evolution of Lactiplantibacillus plantarum enhances furan tolerance and lactic acid production in lignocellulosic hydrolysate.","authors":"Sharoni Sharma, Sarvesh V Surve, Joyleen M Fernandes, Vinit Mahale, Monty Vijayvargiya, Ram Kulkarni","doi":"10.1186/s40643-026-01106-4","DOIUrl":"10.1186/s40643-026-01106-4","url":null,"abstract":"<p><p>Furfural and 5-hydroxymethylfurfural (HMF) are two major lignocellulosic growth inhibitors that hinder microbial growth and fermentation of lignocellulosic hydrolysate for lactic acid production. In this study, we employed adaptive laboratory evolution (ALE) to enhance the tolerance of Lactiplantibacillus plantarum JGR2, a strain previously isolated in our lab, to furfural and HMF. The adapted strains demonstrated significantly improved growth in the presence of these inhibitors compared to the parental strains. Whole-genome resequencing revealed multiple mutations including high-impact non-conservative mutations in genes encoding DNA recombination and repair protein (RecF, lp_0005), flavin prenyltransferase (UbiX lp_0271), and oligo-1,6-glucosidase (lp_0189). Transcriptomic analysis indicated that adaptation elicited more pronounced differential gene expression compared to acute inhibitor exposure. Upon furfural exposure, the furfural-adapted isolate showed fewer differentially expressed genes than the parental strain, indicating a possible shift in the transcriptomic profile as a possible mechanism of furfural adaptation. Mechanistic investigation revealed that the adapted isolates reduce furfural into the less toxic furfuryl alcohol, suggesting a key detoxification mechanism. Notably, lp_3051 (dhaT, 1,3-propanediol dehydrogenase) encoding furfural reductase activity was upregulated in both furfural- and HMF-adapted isolates. Membrane fatty acid analysis revealed increased unsaturated fatty acids and cyclopropane fatty acids in adapted strains. Finally, the adapted strains exhibited improved growth in rice straw hydrolysate and produced significantly higher relative lactic acid yields compared to the parental strain, thus demonstrating improved bioproduction under inhibitor-rich conditions. This study not only provides a comprehensive understanding of L. plantarum's response to lignocellulosic inhibitors, but also yields evolved bacterial candidates for further scientific and industrial exploration.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13433676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized melanin production from marine-associated Bacillus sp. EGY7 as a sustainable precursor of carbon dots for biomedical applications.","authors":"Hadeer Ali, Labiba El-Kohordagui, Ahmed Hussein, Sherif Hammad, Nefertiti El-Nikhely, Nehad Noby","doi":"10.1186/s40643-026-01100-w","DOIUrl":"10.1186/s40643-026-01100-w","url":null,"abstract":"<p><p>Melanin, a multifunctional biopigment with photoprotective, antioxidant, and therapeutic properties, is limited by scarce sustainable sources and poor aqueous solubility. In this study, melanin production from a newly isolated marine-associated Bacillus sp. EGY7 was optimized using a Box-Behnken design, achieving a yield of 0.208 g/L. The microbial melanin was subsequently hydrothermally converted into water-dispersible melanin-derived carbon dots (MCDs) with quasi-spherical morphology, particle size of 9-15 nm, and a quantum yield of 5.05 ± 0.31%. The MCDs exhibit strong UV absorption, tunable and often excitation-dependent photoluminescence (PL), high photostability, and environment-sensitive fluorescence governed by their core-shell structure and surface functional groups. MCDs exhibited moderate antioxidant activity and high cytocompatibility, maintaining > 70% viability in human skin fibroblasts. Compared with native melanin, MCDs demonstrated enhanced selective cytotoxicity against breast cancer cells (IC₅₀: 0.25 mg/mL for MCF-7 and 0.68 mg/mL for MDA-MB-231), with selectivity indices reaching 19.6 and superior inhibition of long-term colony formation. Mechanistic investigations suggested that their anticancer activity is mediated by reactive oxygen species (ROS)-induced apoptosis coupled with efficient nanoscale cellular uptake. Complementary in silico analyses predicted interactions with multiple cancer-related targets involved in apoptosis, oxidative stress regulation, and cell cycle control, supporting their theragnostic potential. Overall, these findings establish Bacillus sp. EGY7 as a renewable melanin source and highlight MCDs as a sustainable multifunctional platform for selective anticancer and bioimaging applications.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13407830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocircular economy-driven bacterial cellulose with large pore size: statistical optimization using Glutamicibacter soli bread waste hydrolysate.","authors":"Fatma Abdul El Hak, Amira A Matrawy, Mohamed S Elnouby, Amira M Embaby, Wael Wazeer, Hoda E Mahmoud","doi":"10.1186/s40643-026-01098-1","DOIUrl":"10.1186/s40643-026-01098-1","url":null,"abstract":"<p><p>Bacterial cellulose (BC) is a versatile biopolymer of considerable biotechnological interest, with wide-ranging applications in biomedical, industrial, and engineering fields attributable to its exceptional structural and physicochemical properties. Despite its promise, the high cost of conventional production media and the challenge of tailoring BC structural characteristics remain critical bottlenecks limiting sustainable large-scale manufacturing. In the present study, a novel fermentation medium based on Glutamicibacter soli bread waste hydrolysate (BWH) was developed and statistically optimized for high-yield BC production by Komagataeibacter sp. strain HIJ12 EMCCN-4085, integrating circular bioeconomy principles through food waste valorization. One-variable-at-a-time (OVAT) screening identified glycerol and ethanol supplementation, incubation at 28 °C, pH 5.5, a 2-day-old inoculum, and an aeration ratio of zbroth: flask volume) as the key determinants governing BC biosynthesis. Subsequent Box-Behnken design-based Response Surface Methodology, supported by canonical and ridge analyses, refined glycerol concentration, inoculum size, and incubation time, yielding an exceptional BC dry weight of 39 g L^-1 within 5.8 days, substantially surpassing most reported waste-based BC systems that typically achieve 4-15 g L^-1 over longer fermentation periods. Structural characterization demonstrated that hydrolysate-based BC possessed a distinctively large pore size, enhanced porosity (41 ± 1.2%), and reduced crystallinity relative to conventional Hestrin-Schramm-based BC, while fully preserving the characteristic cellulose chemical structure, properties particularly advantageous for drug delivery, wound healing, and filtration applications. Collectively, these findings establish G. soli BWH as a high-performance, sustainable fermentation substrate that simultaneously enhances BC productivity and tailors material properties, offering a compelling strategy for converting food waste into high-value biomaterials.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"13 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13407821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}