Bioresources and Bioprocessing最新文献

{"title":"Dendrobium officinale-derived nanovesicles: a natural therapy for comprehensive regulation of angiogenesis, inflammation, and tissue repair to enhance skin wound healing.","authors":"Jin Tu, Luhua Xu, Yuqin Guo, Minzhi Zhang, Miao Gan, Xiuzhen Bao, Rongfeng Yang, Hanjiao Liu, Fengxia Lin","doi":"10.1186/s40643-025-00915-3","DOIUrl":"10.1186/s40643-025-00915-3","url":null,"abstract":"<p><p>Skin wound healing is a multifaceted biological process involving dynamic interactions among various cells and signaling molecules. Angiogenesis is a key component of this repair process. Dendrobium officinale, a traditional medicinal plant, has shown therapeutic promise, particularly through its bioactive nanovesicles. This study investigates the therapeutic potential of Dendrobium officinale-derived nanovesicles (DDNVs) in regulating angiogenesis, inflammation, and tissue repair, to promote enhanced skin wound healing. A full-thickness mouse skin wound model was used to evaluate the in vivo effects of DDNVs on wound closure, angiogenesis, and collagen remodeling. Histological staining (H&E and Masson's trichrome) and CD31 immunofluorescence were performed. In vitro, DDNVs were tested on Human umbilical vein endothelial cells(HUVECs) and Human keratinocyte cells (HaCaT) cells to assess cell proliferation, migration, and angiogenesis. Confocal microscopy was used to track cellular uptake. Activation of the Akt/eNOS pathway and expression of key genes related to inflammation and matrix remodeling were evaluated by Western blotting and qPCR. DDNVs significantly accelerated wound healing and promoted angiogenesis in vivo, as evidenced by enhanced CD31 expression and collagen remodeling. In vitro, DDNVs entered cells efficiently and stimulated HUVEC and HaCaT proliferation and migration. This was accompanied by activation of the Akt/eNOS signaling pathway, increased expression of eNOS and VEGFR-2, upregulation of extracellular matrix(ECM) components (Vimentin, Fibronectin, COL1A1), and suppression of inflammatory markers such as ICAM-1 and IL-1β. DDNVs exhibit strong potential to enhance skin wound healing by promoting angiogenesis, improving tissue repair, and modulating inflammation. These findings support the clinical development of DDNVs as a novel, plant-derived nanotherapeutic for chronic wound treatment and skin regeneration.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"74"},"PeriodicalIF":4.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of conjugated linoleic acid: current status and future perspectives.","authors":"Lu Lin, Mei-Li Sun, Kaifeng Wang, Jian Gao, Xiao-Jun Ji, Quanyu Zhao","doi":"10.1186/s40643-025-00911-7","DOIUrl":"10.1186/s40643-025-00911-7","url":null,"abstract":"<p><p>Conjugated linoleic acid (CLA) has received much attention for its beneficial physiological effects, particularly anticancer and metabolic control activity. However, traditional sources rely on extraction and chemical synthesis, both of which are not commercially viable. This article describes the research progress on sustainable alternative biosynthesis methods for the production of CLA, which is an attractive approach for commercial production because of its high stereoselectivity and convenient purification process. Here, we offer a comprehensive review of the current state and research progress in the biosynthesis of CLA, including production strains, biosynthesis mechanisms and improvements in the biosynthesis of CLA by genetic engineering. Finally, future research directions for improving the biosynthesis of CLA are defined in the light of recent advances, barriers and trends in this field.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"72"},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing polyphenols from pulp industry residues of juvenile eucalyptus wood: potential for adhesive applications.","authors":"Lucía Xavier, Rodrigo Coniglio, Fabián Bermúdez, Diego Passarella, Leonardo Clavijo","doi":"10.1186/s40643-025-00914-4","DOIUrl":"10.1186/s40643-025-00914-4","url":null,"abstract":"<p><p>Approximately 1-2% of the wood processed for pulp production is converted into sawdust and pinchips, which are commonly burned for energy in industries that already produce excess power. This material, juvenile wood only containing sapwood, poses challenges for its valorization. This study explores the extraction of polyphenols from Eucalyptus wood residues generated by a pulp industry in Uruguay, aiming to evaluate their suitability as partial replacements in phenol-formaldehyde (PF) adhesives. Using solid-liquid extraction with sodium hydroxide and sodium sulfite, the extractions conditions were optimized through an experimental design considering sodium hydroxide and sodium sulfite charges, along with temperature as variables. The extracts were characterized based on phenolic content, condensed tannins content, antioxidant activity, and Stiasny number, to assess the adhesive potential. Adhesives formulated with 10%, 20%, and 30% PF resin substitution demonstrated a progressive reduction in tensile shear strength, with the 10% substitution achieving acceptable strength. While higher substitution levels compromised adhesive performance, the results suggest that formulation adjustments may improve performance at moderate substitution ratios. Furthermore, the high antioxidant capacity of the extracts opens up potential for their use in other high-value applications where natural antioxidants are increasingly sought after as safer and more sustainable alternatives to synthetic additives.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"73"},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Changes in microbial composition during flue-cured tobacco aging and their effects on chemical composition: a review.","authors":"Yangyang Yu, Yongfeng Yang, Tao Jia, Hang Zhou, Yao Qiu, Mengyao Sun, Hongli Chen","doi":"10.1186/s40643-025-00913-5","DOIUrl":"10.1186/s40643-025-00913-5","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"71"},"PeriodicalIF":4.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antioxidant activity of brown seaweed Laminaria japonica by fermentation using isolated Bacillus subtilis.","authors":"Yueh-Hao Ronny Hung, Ya-Han Chang, Hsuan-Ju Lin, Li-Ho Chiang, Hong-Ting Victor Lin","doi":"10.1186/s40643-025-00912-6","DOIUrl":"10.1186/s40643-025-00912-6","url":null,"abstract":"","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"69"},"PeriodicalIF":4.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial lipid-based biodiesel production using wastewater: opportunities and challenges.","authors":"Ameera Al Shehhi, Yasmine Souissi, Anu Sadasivan Nair, Zeba Usmani, Minaxi Sharma, Nallusamy Sivakumar","doi":"10.1186/s40643-025-00897-2","DOIUrl":"10.1186/s40643-025-00897-2","url":null,"abstract":"<p><p>Rising environmental concerns and fossil fuel depletion necessitate the search for sustainable, alternative energy sources. Biodiesel is emerging as a viable alternative owing to its biodegradability, low toxicity, and reduced greenhouse gas emissions. This review highlights the environmental and economic advantages of biodiesel, with a special emphasis on microbial lipids from wastewater as a promising third-generation feedstock. The novelty of this review is the comprehensive evaluation of wastewater and sewage sludge as low-cost, nutrient-rich substrates for microbial lipid production. In this review, the biodiesel production by direct lipid extraction and microbial conversion routes is discussed. Biodiesel production from different types of industrial wastewaters, such as municipal, dairy, pulp and paper, brewery, and textile wastewater using oleaginous microorganisms is discussed as well. This review revealed that lipid content in different microbes ranged from 27 to 90% when different wastewaters were used as substrates. Further, this review compares the fuel properties of biodiesel derived from plant oils, animal fats, and microbial lipids, their fatty acid profiles, and estimated production costs, and it varies from 0.1 to 0.83 USD/L. In addition, this review also focuses on the biodiesel production from wastewater using microalgae, fungi, and bacteria. Biodiesel with a high cetane number of 64.47 was produced by Cryptococcus curvatus. Finally, key challenges in producing biodiesel from wastewater and future prospects are discussed, emphasizing the potential of wastewater as a substrate for sustainable biodiesel production.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"70"},"PeriodicalIF":4.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of immobilized mannanase on polyhydroxyalkanoate (PHA).","authors":"Zhiyue Men, Yafei Zhang, Zhao Pang, Tao Li, Hening Zhang, Yue Zhou, Ning Hao, Yajian Song, Yu Bai","doi":"10.1186/s40643-025-00886-5","DOIUrl":"10.1186/s40643-025-00886-5","url":null,"abstract":"<p><p>Galactomannan oligosaccharides (GMOS), composed of 2-10 mannose units linked with β-1, 4 glycosidic bond as the main chain and galactose linked with α-1, 6 glycosidic bond as the side chain, are crucial for probiotic food synthesis due to their ability to promote the growth and activity of beneficial intestinal microbiota, enhance the host immune system, and improve nutrient digestion. GMOS is usually obtained by hydrolyzing plants such as locust bean gum and guar gum with mannanase. β-mannanase ManA from Alkaliphilic Bacillus sp. N16-5 can hydrolyze β-1, 4 glycosidic bond of galactomannan. In this study, an immobilization system was employed utilizing polyhydroxyalkanoate (PHA) biopolymers, which naturally have an affinity mainly mediated by hydrophobic interaction for PhaP protein. Fusion protein combining ManA with PhaP from Aeromonas hydrophila, was subsequently immobilized on PHA support to form a multi-enzyme complex, facilitating the hydrolysis of locust bean gum to generate GMOS. This immobilized enzyme enhances enzyme stability and reusability, can be reused up to 32 times while maintaining ~ 80% of its activity, offering substantial cost savings through in-situ enzyme and product separation. Additionally, the different PHA forms were developed to hydrolyze locust bean gum to produce GMOS, such as nano PHA particles, PHA electrospun materials, while these preliminary investigations show promise, further research is needed to optimize their performance and practical application.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"68"},"PeriodicalIF":4.3,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic potential and analytical profiling of plant probiotic bacteria using chromatography and mass spectrometry techniques against Botrytis cinerea and Fusarium oxysporum.","authors":"Gottumukkala Hiranmayee, Sarada Prasanna Mallick, Golamari Siva Reddy","doi":"10.1186/s40643-025-00853-0","DOIUrl":"10.1186/s40643-025-00853-0","url":null,"abstract":"<p><p>Plant probiotics are bacteria that play a significant role in enhancing plant growth and health. To understand the interactions between plant probiotics and host plants, a comprehensive approach of antagonistic activity and analytical methods such as high-performance liquid chromatography (HPLC), gas chromatography‒mass spectrometry (GC‒MS), and Fourier transform infrared (FT‒IR) spectroscopy, were employed. The previously isolated bacterial strains, namely, Corynebacterium accolens strain CNTC Th1/57, Bacillus rugosus strain SPB7, Lactobacillus pasteurii DSM 23907 and Cytobacillus firmus strain NBRC 15306, were exposed to antagonistic testing against Botrytis cinerea and Fusarium oxysporum. Considering the results of the antagonistic activity both in vitro and statistically, the bacterial strains Bacillus rugosus strain SPB7 and Lactobacillus pasteurii DSM 23907 presented greater zones of inhibition. Hence these bacteria were moved to obtain comprehensive insights into the chemical composition. HPLC and GC‒MS resulted in the identification of phenols and organic acids. These results were further confirmed by FT-IR, which revealed a peak at 3500 cm^-1 for Bacillus rugosus strain SPB7, where O-H, aromatic C-H and aromatic C = C stretching vibrations were also observed at 3069 and 1549 cm^-1. The peak at 1736 cm^-1 corresponds to the carboxyl group (-COOH) as the functional group with respect to Lactobacillus pasteurii DSM 23907. Further confirmation was performed by observing the other absorption bands at 3451 cm^-1 and 2958 cm^-1, indicating the presence of hydroxyl group (O-H) and alkyl group (C-H) stretching vibrations, thus confirming their potential for the production of phenols and organic acids, respectively, by bacteria. This findings would make a way to explore plant diseases, tolerance against pathogens, and also study ecological role of these bacteria in plant communities.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"67"},"PeriodicalIF":4.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oil palm residue-based cellulose acetate membranes enhanced with zinc oxide and n-methyl pyrrolidinone for batik wastewater treatment.","authors":"Belladini Lovely, Hasna Amalia Fauziyyah, Shendy Krisdayanti, Muhamad Zakky Irsyada, Lisna Efiyanti, Wara Dyah Pita Rengga, Novitri Hastuti, R A Ilyas, Mohd Nor Faiz Norrrahim, Victor Feizal Knight","doi":"10.1186/s40643-025-00880-x","DOIUrl":"10.1186/s40643-025-00880-x","url":null,"abstract":"<p><p>As the world's top producers of oil palm (Elaeis guineensis), Indonesia and Malaysia are urged to propose a value-added valorization of its lignocellulosic biomass, oil palm empty fruit bunches (OPEFB). Meanwhile, the nations' signature 'batik' textile industries are in dire need of optimum remediation treatments of their wastewater high in harmful dyes and chemicals. Organic-inorganic hybrid systems of mixed matrix membranes (MMMs) for heavy metals removal were prepared using OPEFB-based cellulose acetate (CA) and zinc oxide (ZnO; 0.5, 0.75, 1%, w/v) in N-methyl pyrrolidinone (NMP; 89, 90, 91%, v/v). The high crystallinity (62.42%) and fibrils' web-like structure of OPEFB-CA were confirmed. Microscopic observation of OPEFB CA-NMP-ZnO membranes evidenced the porous yet smooth surface due to the use of plasticizing NMP, as well as uniform dispersion of ZnO particles. MMM 2 (0.75%ZnO; 90%NMP) was the best-performing membrane mechanically with excellent tensile strength (1.78 MPa), Young's modulus (0.13 GPa), and elongation-at-break (2.59%), while thermal stability (T_d,5%, 291 °C) improvement was also highlighted. Pores characteristics on size, volume, and surface area were discussed, too. Remediation performance was excellent even at high (20%) effluent concentration reaching 28% and 65% removal of Cu and Pb, respectively, by MMM 1 (0.5%ZnO; 89%NMP). These findings confirmed the promising prospect of the developed membranes as a wastewater remediation treatment, including in textile industries.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"64"},"PeriodicalIF":4.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation of beechwood xylan using food-grade bacteria-like particles displaying β-xylosidase from Limosilactobacillus fermentum.","authors":"Robie Vasquez, Ji Hoon Song, Jae Seung Lee, Bernadette Bagon, Sanghoon Kim, Valerie Diane Valeriano, Dae-Kyung Kang","doi":"10.1186/s40643-025-00898-1","DOIUrl":"10.1186/s40643-025-00898-1","url":null,"abstract":"<p><p>The display of enzymes on bacterial surfaces is an interesting approach for immobilising industrially important biocatalysts. In recent years, non-recombinant surface display using food-grade bacteria, such as lactic acid bacteria (LAB), have gained interest because of their safety, simplicity, and cost-effectiveness. β-Xylosidase is one of the many biocatalytic enzymes targeted for immobilisation due to its key role in the complete saccharification of lignocellulosic biomass, including xylan hemicellulose. Recently, the xylose-tolerant β-xylosidase, LfXyl43, was identified in Limosilactobacillus fermentum. LfXyl43 is capable of producing xylose from the degradation of xylo-oligosaccharides (XOS) and beechwood xylan. This study aimed to immobilise this new biocatalyst on the surface of LAB-derived bacteria-like particles (BLP) and investigate its applicability and reusability in the degradation of xylan hemicellulose. Additionally, the influence of the anchor position and the presence of linker peptides on the display and activity of the β-xylosidase was investigated. Four expression vectors were constructed to express different anchor-xylosidase fusion proteins. Upon expression and purification, all anchor-xylosidase fusion proteins were active towards the artificial substrate p-nitrophenyl-β-D-xylopyranoside. In addition, all anchor-xylosidase fusion proteins were successfully displayed on the surface of BLP. However, only the β-xylosidases with linker peptide showed hydrolytic activity after immobilisation on BLP. BLP displaying β-xylosidases demonstrated high activity against XOS and beechwood xylan, thereby producing high amounts of xylose. Moreover, the immobilised enzyme demonstrated reusability across several bioconversion cycles. Overall, this study highlights the potential industrial application of surface-displayed β-xylosidase for the effective degradation of lignocellulosic biomass.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"12 1","pages":"66"},"PeriodicalIF":4.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}