Recombinant Escherichia coli-driven whole-cell bioconversion for selective 5-Aminopentanol production as a novel bioplastic monomer.

IF 5.1 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Byung Wook Lee, Hee Taek Kim, Hyun Gi Koh, Kyungjae Yu, Gaeul Kim, Yoon Jung Jung, Haeng-Geun Cha, Yunhee Jeong, Yung-Hun Yang, See-Hyoung Park, Kyungmoon Park
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引用次数: 0

Abstract

5-Aminopentanol (5-AP) is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics. Conventional production methods rely on petroleum-based feedstocks and metal catalysts, which raise environmental and sustainability concerns. In this study, a de novo biosynthetic pathway for 5-AP production from L-lysine was developed in Escherichia coli. The engineered pathway consisted of lysine decarboxylase 2 (LdcC), putrescine aminotransferase (PatA), and tested aldehyde reductase (YahK, YihU, YqhD). Among the tested reductases, aldehyde reductase exhibited the highest catalytic efficiency, producing 44.5 ± 2.6 mM of 5-AP (0.44 ± 0.03 mol5 - AP/moll-lysine). The replacement of the expression system with a T7-based dual-plasmid platform, pET24ma::ldcC, and pCDFDuet-1::yqhD::patA co-transformed into E. coli, increased the production to 60.7 ± 5.8 mM, accompanied by reduced cadaverine accumulation. Further enhancement was achieved by increasing the gene dosage of PatA, leading to 68.5 ± 4.2 mM 5-AP and reduced by 40% in cadaverine levels. Cadaverine is a precursor in the production of 5-AP, and its accumulation is an important factor in the limitation of conversion to 5-AP. Intracellular cofactor regeneration is expected to cause an indirect supply of α-KG, a cofactor, to enhance conversion to 5-AP. To support intracellular cofactor regeneration, glucose supplementation and increased aeration were applied, resulting in a final titer of 78.5 ± 1.2 mM 5-AP and improved precursor utilization. This study is the first report of selective microbial 5-AP production and highlights the importance of PatA expression in pathway optimization. The newly established L-lysine (C6) valorization process which converts L-lysine to high-value materials such as 1,5-PDO, glutarate, and 5-AP offers a promising route for the sustainable biosynthesis of amino alcohols, laying the groundwork for future improvements through enzyme engineering and metabolic design.

重组大肠杆菌驱动的全细胞生物转化选择性生产5-氨基戊醇作为一种新的生物塑料单体。
5-氨基戊醇(5-AP)是一种有价值的氨基醇,在高分子合成和生物塑料方面具有潜在的应用前景。传统的生产方法依赖于石油基原料和金属催化剂,这引起了环境和可持续性问题。本研究在大肠杆菌中建立了l -赖氨酸生产5-AP的新生物合成途径。该工程途径由赖氨酸脱羧酶2 (LdcC)、腐胺转氨酶(PatA)和被测醛还原酶(YahK, YihU, YqhD)组成。在所有的还原酶中,醛还原酶的催化效率最高,产生44.5±2.6 mM的5-AP(0.44±0.03 mol5 -AP /moll-赖氨酸)。将pET24ma::ldcC和pCDFDuet-1::yqhD::patA共转化到大肠杆菌中,将表达系统替换为t7双质粒平台,产量增加到60.7±5.8 mM,同时尸胺积累减少。通过增加PatA基因剂量进一步增强,导致68.5±4.2 mM 5-AP,尸胺水平降低40%。尸胺是5-AP生成的前体,其积累是限制5-AP转化的重要因素。细胞内辅助因子再生有望导致α-KG(一种辅助因子)的间接供应,以增强向5-AP的转化。为了支持细胞内辅助因子再生,葡萄糖补充和增加曝气,最终滴度为78.5±1.2 mM 5-AP,提高了前体利用率。本研究首次报道了选择性微生物5-AP的产生,强调了PatA表达在途径优化中的重要性。新建立的l -赖氨酸(C6)增值工艺将l -赖氨酸转化为1,5- pdo、戊二酸和5-AP等高价值物质,为氨基醇的可持续生物合成提供了一条有前途的途径,为未来通过酶工程和代谢设计进行改进奠定了基础。
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来源期刊
Bioresources and Bioprocessing
Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
8.70%
发文量
118
审稿时长
13 weeks
期刊介绍: Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
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