促进细菌定植和生物膜形成,以增强低密度聚乙烯微塑料的生物降解。

IF 5.1 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Marwa Gamal Eldeen Afify, Ola M Gomaa, Hussein Abd El Kareem, Mohamed A Abou Zeid
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引用次数: 0

摘要

塑料垃圾的堆积是一个全球性的重大环境挑战。本研究旨在从含有塑料废物的海洋样品中分离聚乙烯降解细菌。四种可培养的细菌分离物:黄体微球菌、蜡样芽孢杆菌、粪肠球菌和放线菌,对它们的生物膜形成、生物表面活性剂和蛋白酶生产进行了评估。采用伽马辐射诱导低密度聚乙烯微塑料(LDPE MPs)的结构变化,促进细菌定植和生物膜形成。在添加30%色氨酸豆汤、10%生物表面活性剂和300µM氯化钙的培养基中,生物膜形成效果最佳。因子设计实验表明,与γ辐照相比,添加培养基可显著改善细菌定植和生物膜形成。扫描电子显微镜(SEM)、能量色散x射线(EDX)测绘和傅里叶变换红外光谱(FTIR)支持了这一研究。优化的LDPE MP降解通过多步骤方案实现:(1)样品预处理到40 kGy的伽马辐射,导致5.7%的重量损失和结构和形态变化,(2)在生物膜诱导培养基中孵育过夜,(3)在最小培养基中进一步孵育30天。这种方法使总体重减轻了22.5%。综上所述,协同预处理可促进生物膜形成,提高海洋细菌对LDPE MPs的生物降解能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Promoting bacterial colonization and biofilm formation for enhanced biodegradation of low-density polyethylene microplastics.

The accumulation of plastic waste presents a significant worldwide environmental challenge. This study aimed to isolate polyethylene-degrading bacteria from marine samples containing plastic waste. Four culturable bacterial isolates: Micrococcus luteus, Bacillus cereus, Enterococcus faecalis, and Actinomyces sp. were assessed for their biofilm formation, biosurfactant, and protease production. Gamma irradiation was used to induce structural changes and promote bacterial colonization and biofilm formation on low-density polyethylene microplastics (LDPE MPs). Optimal biofilm formation was achieved in minimal media supplemented with 30% tryptic soy broth, 10% biosurfactant, and 300 µM calcium chloride. The factorial design experiment demonstrated that adding media supplementation significantly improved bacterial colonization and biofilm formation when compared to gamma irradiation. This was supported with Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) mapping, and Fourier Transform Infrared Spectroscopy (FTIR). The optimized LDPE MP degradation was achieved through a multi-step protocol: (1) samples are pre-treated to 40 kGy gamma irradiation, which resulted in 5.7% Gravimetric weight loss and structural and morphological changes, (2) incubation in biofilm inducing media overnight, and (3) further incubation in minimal media for 30 days. This approach resulted in a total weight loss of 22.5%. In conclusion, synergistic pre-treatment is recommended to promote biofilm and improve biodegradation of LDPE MPs by marine bacteria.

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来源期刊
Bioresources and Bioprocessing
Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
8.70%
发文量
118
审稿时长
13 weeks
期刊介绍: Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
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