Biointerphases最新文献

Dissecting the role of substrate folding in enzymatic digestion. 剖析底物折叠在酶消化中的作用。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004803

Nilimesh Das, Tanmoy Khan, Soumya Chaudhury, Bhaswati Sengupta, Pratik Sen

{"title":"Dissecting the role of substrate folding in enzymatic digestion.","authors":"Nilimesh Das, Tanmoy Khan, Soumya Chaudhury, Bhaswati Sengupta, Pratik Sen","doi":"10.1116/6.0004803","DOIUrl":"10.1116/6.0004803","url":null,"abstract":"The efficiency of enzymatic proteolysis is often attributed to the properties of the enzyme itself, with the substrate typically viewed as a passive participant. In this study, we demonstrate that the conformational state of the substrate critically influences proteolytic efficiency. Using human serum albumin (HSA) as a model substrate, papain as the enzyme, and urea as a controlled denaturing agent, we systematically investigated how substrate conformation might affect proteolysis. While papain maintains its structural and functional integrity across varying urea concentrations, HSA transitions through well-defined conformational states (native, compact intermediate, and unfolded), allowing us an opportunity to isolate the effects of the substrate structure. Utilizing site-specific fluorescent labeling and single-molecule fluorescence correlation spectroscopy, we monitor the progression of proteolysis. Our results show that digestion slows at 3M urea, where HSA adopts a compact form, and accelerates at 6M, where HSA takes on an unfolded state, compared to native HSA. These results reveal that substrate folding critically influences the digestion kinetics, probably by controlling protease accessibility and underscoring its importance in mechanistic enzymology and proteomics workflows.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human osteoblast derived biochemical cues and microsurface topography modulate osteogenesis in vitro and in vivo. 人成骨细胞衍生的生化线索和微表面地形在体外和体内调节成骨。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004679

Deepa Mishra, Anne Bernhardt, Michael Gelinsky, Bikramjit Basu

{"title":"Human osteoblast derived biochemical cues and microsurface topography modulate osteogenesis in vitro and in vivo.","authors":"Deepa Mishra, Anne Bernhardt, Michael Gelinsky, Bikramjit Basu","doi":"10.1116/6.0004679","DOIUrl":"https://doi.org/10.1116/6.0004679","url":null,"abstract":"Indirect co-culture, wherein two distinct cell types are cultivated within the same medium without direct contact, remains a relatively underexplored approach in biomaterials science for simulating physiological cell-cell interactions on material surfaces in vitro. In this study, human mesenchymal stem cells (hMSCs) were cultured on two types of Ti6Al4V substrates (polished and sand-blasted/acid etched) in a co-culture system using conditioned osteogenic differentiation media (cOBM), enriched with soluble factors secreted by human osteoblasts (hOBs). The combined impact of surface microtopography of Ti6Al4V substrates and cOBM supplementation has resulted in the modulation of cell morphology, alkaline phosphatase (ALP) activity, and calcium phosphate mineralization. Enhanced mineralization (2.5-fold increase compared to baseline at day 21) was observed on Ti6Al4V substrates when hMSCs were cultured in the presence of cOBM. This was accompanied by a peak expression of the early osteogenic marker, ALP by day 14. The synergistic behavior of sandblasted and acid-etched substrates with soluble biochemical cues, derived from hOBs showcased their potential for augmenting osteogenic differentiation. The in vitro outcomes were validated in a rabbit model study, which clearly demonstrated better osseointegration of sand-blasted/acid etched implants over 12 weeks.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical methods to study the complex dynamics of biofilm-biomaterial interfaces. 研究生物膜-生物材料界面复杂动力学的分析方法。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004613

Yunxing Li, Dipankar Koley

{"title":"Analytical methods to study the complex dynamics of biofilm-biomaterial interfaces.","authors":"Yunxing Li, Dipankar Koley","doi":"10.1116/6.0004613","DOIUrl":"https://doi.org/10.1116/6.0004613","url":null,"abstract":"Biofilm-biomaterial interfaces have an important role in biofilm development and pose a critical challenge in healthcare, contributing to device failures and chronic infections that affect patient outcomes and healthcare economics. This review explores the complex dynamics of these interfaces, from initial protein adsorption through mature biofilm development, highlighting how bacteria and materials are involved in bidirectional interactions that determine both infection progression and material degradation. It also examines different advanced analytical methods for characterizing these dynamic biofilm-biomaterial interactions, with particular emphasis on the recent developments in electrochemical techniques (ion-selective electrodes, electrochemical impedance spectroscopy, and scanning electrochemical microscopy) that enable real-time monitoring of critical parameters such as pH, oxygen gradients, and metabolic activities, providing unique insights into biofilm heterogeneity and localized chemical changes. In addition, the review explores future developments in sensor technology and standardized protocols needed to accelerate biomaterial innovation, potentially transforming our approach to implant-associated infections through responsive surfaces that adapt to microbial challenges.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zeolite substrate characterization for Metarhizium robertsii inoculation. 罗伯特绿僵菌接种的沸石基质表征。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004518

Ángel Guillén-Cervantes, Francisco Hernández-Rosas, Blas Schettino-Salomón, José Alberto Aguilar-Ayala, Felipe Francisco Muñoz-Ponce, Juan Hernández-Rosas

{"title":"Zeolite substrate characterization for Metarhizium robertsii inoculation.","authors":"Ángel Guillén-Cervantes, Francisco Hernández-Rosas, Blas Schettino-Salomón, José Alberto Aguilar-Ayala, Felipe Francisco Muñoz-Ponce, Juan Hernández-Rosas","doi":"10.1116/6.0004518","DOIUrl":"https://doi.org/10.1116/6.0004518","url":null,"abstract":"For this study, zeolite powder served as a substrate for inoculating Metarhizium robertsii to demonstrate the biocompatibility between the entomopathogenic fungus and the zeolite mineral, as the initial step in developing a biological control agent. Our fungal strains were isolated from corpses of spittlebugs (Aeneolamia albofasciata, Hemiptera: Cercopidae) and were identified as M. robertsii based on sequencing of the Internal Transcribed Spacer regions ITS1 and ITS2. Zeolite was characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). XRD and EDS results indicate that zeolite consists of a mixture of Heulandite and Clinoptilolite. EDS analysis shows that oxygen, silicon, and aluminum are the primary chemical components of the zeolite powder, with calcium, magnesium, iron, sodium, and potassium present in smaller amounts. After five days of inoculation, SEM images reveal M. robertsii conidia on the porous surface of zeolite particles, along with hyphal formation. These findings suggest the potential for maintaining M. robertsii spores and mycelium alive within a zeolite substrate under laboratory conditions.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of vacuum exposure on sample stability for mass spectrometry analysis. 真空暴露对质谱分析样品稳定性的影响。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004632

Matija Lagator, Siyu Liu, C Logan Mackay, Felicia Green

{"title":"Effects of vacuum exposure on sample stability for mass spectrometry analysis.","authors":"Matija Lagator, Siyu Liu, C Logan Mackay, Felicia Green","doi":"10.1116/6.0004632","DOIUrl":"https://doi.org/10.1116/6.0004632","url":null,"abstract":"Mass spectrometry (MS) often requires vacuum conditions, which, while beneficial for analysis, can unpredictably alter sensitive samples. This study investigates the impact of prolonged vacuum exposure on the consistency and reliability of MS detection of thin films of acetaminophen using secondary ion mass spectrometry (SIMS). Under vacuum at room temperature, the mass spectrometry signal intensity decreased by approximately 81.5% over the duration of the experiment (42 h). Optical microscopy revealed that this decrease coincided with sublimation-induced sample loss of the acetaminophen. As a result, acetaminophen coverage across the substrate became heterogeneous, leading to increased relative standard deviation (RSD) in the SIMS signal over time. In contrast, under cryogenic conditions, neither signal degradation nor an increase in RSD was observed. Additionally, a comparison with atmospheric pressure mass spectrometry revealed that, in the absence of vacuum, signal intensity remained more stable over time. These findings highlight the potential drawbacks of vacuum exposure for volatile standards and emphasize the importance of testing vacuum effects prior to analysis. If vacuum is necessary, cryogenic conditions should be considered to mitigate sample degradation. While these effects were observed for a mass spectrometry technique, they are also applicable to any type of vacuum-based methodology where the samples might be prone to sublimation.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced collagenogenesis on three-dimensionally printed titanium surfaces by human gingival fibroblasts: An in vitro study. 人牙龈成纤维细胞增强三维打印钛表面胶原生成的体外研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004500

Vitor de Toledo Stuani, Isabela Sanches Pompeo da Silva, Gustavo Gonçalves do Prado Manfredi, Fernanda Balestrero Cassiano, Larissa Alamo, Ligia Espoliar Corrêa, Jamil Awad Shibli, Carlos Alberto de Souza Costa, Diana Gabriela Soares

{"title":"Enhanced collagenogenesis on three-dimensionally printed titanium surfaces by human gingival fibroblasts: An in vitro study.","authors":"Vitor de Toledo Stuani, Isabela Sanches Pompeo da Silva, Gustavo Gonçalves do Prado Manfredi, Fernanda Balestrero Cassiano, Larissa Alamo, Ligia Espoliar Corrêa, Jamil Awad Shibli, Carlos Alberto de Souza Costa, Diana Gabriela Soares","doi":"10.1116/6.0004500","DOIUrl":"https://doi.org/10.1116/6.0004500","url":null,"abstract":"The lack of cementum in peri-implant tissues leads to a deficiency in anchorage points for gingival collagen fibers. This arrangement is linked to reduced protective capabilities compared to teeth. Therefore, there is a pressing need to develop surfaces that optimize the interaction between soft tissue and implants. 3D-printed titanium disks (Ti3DP), machined disks (TiMC), and glass coverslips (GS) were seeded with human gingival fibroblasts. These specimens underwent mechanical characterization via roughness and wettability assays. Biological characterization included assessments of cellular viability (live/dead), adhesion and spreading (F-actin), cell count (DAPI), cellular metabolism (Alamar blue), adhesive strength, and soluble collagen and total protein quantification up to 14 days. Data analysis employed Student's t-test and ANOVA post-hoc Tukey test (α = 0.05). The group TiMC exhibited higher hydrophilicity and lower roughness compared to Ti3DP. All groups demonstrated cellular viability throughout the study period. Adhesive strength did not significantly differ among groups; however, cell count was higher in TiMC and GS after one day of cell seeding in comparison to Ti3DP. Morphologically, GS and TiMC displayed more fusiform cells with a uniform distribution, while Ti3DP showed smaller, irregular cells with multiple lamellipodia and filopodia. Additionally, statistically superior collagen and total protein deposition was observed in Ti3DP (p < 0.01). The 3D-printed titanium surface allowed human gingival fibroblasts to adhere to it, leading to a 3D cytoskeleton morphology that culminated in increased collagen expression. Therefore, these 3D-printed devices present a promising avenue for producing transmucosal components due to their increase in collagen production.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface functionalization of polycaprolactone-based biomaterials: Good practice and pitfalls. 聚己内酯基生物材料的表面功能化：良好的实践和缺陷。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004773

Alexandra L Mutch, María Natividad Gómez-Cerezo, Lisbeth Grøndahl

{"title":"Surface functionalization of polycaprolactone-based biomaterials: Good practice and pitfalls.","authors":"Alexandra L Mutch, María Natividad Gómez-Cerezo, Lisbeth Grøndahl","doi":"10.1116/6.0004773","DOIUrl":"https://doi.org/10.1116/6.0004773","url":null,"abstract":"Poly(ɛ-caprolactone) (PCL) remains widely studied in biomaterials science and biomedical engineering due to its versatility and applicability in regenerating a range of tissues including bone, cartilage, neural, and cardiovascular. Due to the hydrophobicity of PCL, most PCL based systems for tissue regeneration require a surface modification process to enhance its in vitro and in vivo compatibility. This Perspective aims to provide an overview of recent strategies used to modify 2D films and 3D scaffolds and the associated methods used to characterize these surfaces. The scope is restricted to physical and chemical postmodification methods, excluding blends and composites, to better isolate the effects of surface chemistry. By analyzing the latest studies (published in 2022-2024), we classified the most commonly employed surface modification techniques, and we identified that the surface evaluation of tailored PCL remains a critical challenge in terms of both chemical and morphological characterization as well as the stability of the introduced surface layer/coating. This status of recent literature highlights current excellent practices and characterization methodologies and suggests approaches for refining surface engineering methods of PCL-based biomaterials in the future.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging biomedical applications of herbal extracts-based biomaterials. 基于草药提取物的生物材料的新兴生物医学应用。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004748

Jianling Mo, Haolu Shi, Kefeng Ren, Zhaoyang Chen, Xia Sheng

{"title":"Emerging biomedical applications of herbal extracts-based biomaterials.","authors":"Jianling Mo, Haolu Shi, Kefeng Ren, Zhaoyang Chen, Xia Sheng","doi":"10.1116/6.0004748","DOIUrl":"10.1116/6.0004748","url":null,"abstract":"Bioactive herbal extracts have garnered significant attention due to their multitarget regulation and low toxicity, yet their clinical applications are limited by poor solubility, low bioavailability, and insufficient targeting. This review systematically summarizes the pharmacological properties of terpenoids, alkaloids, flavonoids, polysaccharides, and other components, and explores their synergistic integration with biomaterials such as nanoparticle delivery systems, microneedles, and hydrogels. Functionalized nanocarriers enhance the stability and targeting efficiency of paclitaxel, berberine, and other bioactive herbal extracts. Microneedle technology leverages physical penetration and sustained-release mechanisms to achieve efficient transdermal delivery of bioactive herbal extracts (e.g., aconitine, curcumin, and similar agents). Smart hydrogels incorporating active molecules (e.g., baicalin and icariin) achieve spatiotemporal precision in wound healing and osteoarthritis treatment through pH-/enzyme-/reactive oxygen species-responsive release mechanisms. Additionally, the combination of herbal extracts with stents or bone cement expands their potential in cardiovascular and bone regeneration applications. While these integrated systems demonstrate synergistic effects in antitumor, anti-inflammatory, and tissue repair, challenges remain in scalable manufacturing, in vivo metabolic mechanisms, and long-term biosafety. Future research should integrate smart biomaterial designs and multiomics analysis to establish a comprehensive \"component-carrier-efficacy\" development framework, advancing the convergence of bioactive herbal extracts and modern medical science.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms underlying the effects of urea and the structural dynamics of bovine serum albumin. 尿素作用的分子机制及牛血清白蛋白的结构动力学。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004688

Yanis R Espinosa, C Manuel Carlevaro, C Gastón Ferrara

引用次数: 0

Competitive adsorption and functional activity of fibrinogen on polyurethane biomaterials surfaces. 纤维蛋白原在聚氨酯生物材料表面的竞争吸附及功能活性。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004598

Li-Chong Xu, Christopher A Siedlecki

{"title":"Competitive adsorption and functional activity of fibrinogen on polyurethane biomaterials surfaces.","authors":"Li-Chong Xu, Christopher A Siedlecki","doi":"10.1116/6.0004598","DOIUrl":"https://doi.org/10.1116/6.0004598","url":null,"abstract":"An immunological atomic force microscopy technique was used to recognize fibrinogen adsorption and functional activity on polyurethane biomaterial surfaces in the presence of other proteins. The amount of fibrinogen adsorbed on surfaces as recognized by an antifibrinogen polyclonal antibody when in competitive adsorption with human serum albumin (HSA) or human IgG was found to be related to the molar ratio of proteins. A significant decrease in fibrinogen adsorption was observed only when the fraction of smaller proteins reached a threshold value, dependent on smaller protein properties. The functional activity of fibrinogen was measured by a monoclonal antibody recognizing a region containing the dodecapeptide sequence located at the C-terminus of the γ-chain, γ-400-411. Results show that the presence of smaller proteins affected the conformational structure of fibrinogen and increased the availability of platelet binding sites in fibrinogen adsorbed on surfaces. Platelet adhesion was performed on polyurethane surfaces, which were competitively preadsorbed with fibrinogen and HSA. Platelet adhesion correlated well with the functional activity of fibrinogen, measured after competitive adsorption on surfaces. The work suggests that platelet adhesion is not necessarily determined by the amount of adsorbed fibrinogen but is related to the activity of fibrinogen as measured by the availability of the platelet binding sites in the fibrinogen, γ-chain dodecapeptide.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0