Biointerphases最新文献

{"title":"Extreme copper tolerance and genomic adaptation in early-stage marine biofilms.","authors":"Kailey N Richard, Sara M Tuck, Sophie M Colston, Maryssa A Beasley, W Judson Hervey, Kevin Le, Sean M Brown, Katherine J Franz, Kenan P Fears","doi":"10.1116/6.0005640","DOIUrl":"https://doi.org/10.1116/6.0005640","url":null,"abstract":"<p><p>Biofouling, the accumulation of unwanted organisms on submerged assets, presents an ongoing challenge within the maritime industry. Conventional methods to inhibit growth rely on copper-laden (≤75% copper compounds) antifouling coatings. However, the efficacy of these coatings is declining due to the broad emergence of copper-tolerant species. Here, we investigated the mechanisms underlying this decline in antifouling performance. Polyvinyl chloride (PVC) panels coated with a commercial copper ablative paint were deployed in the Chesapeake Bay to collect early-stage biofilms; uncoated PVC panels were deployed as an inert control group. Community profiles of the harvested biofilms were obtained via 16S amplicon sequencing of the V4 and V5 regions. To elucidate genomics information about specific bacteria, primary bacterial colonizers were isolated on marine agar plates and identified through 16S amplicon sequencing and whole genome sequencing. The isolates were subsequently assessed for copper tolerance using copper sulfate (CuSO4) in both marine agar and broth assays. While bacterial tolerance declined with increasing CuSO4 concentrations on agar, tolerance was significantly greater in liquid media and minimum inhibitory concentrations were equal to or above 5 mM CuSO4. Genomic annotation confirmed that this high phenotypic tolerance was driven by active genetic resistance mechanisms. Every isolate harbored at least three copper-related genes (e.g., copA, cueO, and copZ), with highly adaptable species carrying a full spectrum of resistance genes. Ultimately, these findings highlight how the accumulation of metal resistance genes allows bacteria to colonize metal-rich surfaces, paving the way for subsequent macrofouling and coating failure.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mandibular mechanical properties and material composition in a generalist pest insect feeding on different host plants.","authors":"Wencke Krings, Daniel Bernardi, Stanislav N Gorb, Greta Huttegger, Willian Lucena, Larissa Pasqualotto, Karina L Silva-Brandão","doi":"10.1116/6.0005706","DOIUrl":"https://doi.org/10.1116/6.0005706","url":null,"abstract":"<p><p>Adaptations of insect mouthparts to host plants play a key role in species distribution and evolutionary trajectories. We investigated the larval mandibles of the generalist lepidopteran Spodoptera frugiperda, focusing on material composition and mechanical properties in response to different larval food resources. Nanoindentation revealed pronounced functional differentiation within each mandible: the cutting edge exhibited the highest hardness and stiffness values, while basal regions were softer and more flexible, indicating adaptive gradients that balance wear resistance with shock absorption. Confocal laser scanning microscopy revealed a strong relationship between autofluorescence-based sclerotization signals and the gradients in elastic modulus (E) and hardness (H). Elemental composition analyses identified potential cross-linking elements, with zinc (Zn) showing the highest correlations with mechanical properties. Host plant-dependent differences were found, i.e., larvae feeding on silica phytolith-containing host plants (Zea mays, Avena sativa, Oryza sativa) exhibited higher Zn contents, increased degrees of sclerotization, and elevated E and H values, resulting in reduced mandibular wear compared with larvae feeding on plants with weaker mechanical defenses (Amaranthus hybridus, Conyza bonariensis). This intraspecific variation underscores the importance of elemental incorporation and tanning in mediating adaptation to plant mechanical defenses. Variation in mouthpart properties may enhance feeding performance across host plants; however, because all tested individuals were assigned to the C-strain, the present study addresses host-associated variation within a single strain rather than divergence between strains.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling biomaterials manufacturing: Lessons from the paper industry.","authors":"Sandro Zier","doi":"10.1116/6.0005609","DOIUrl":"10.1116/6.0005609","url":null,"abstract":"<p><p>Biomaterials are increasingly being explored as sustainable alternatives to petroleum-derived materials, but many systems that perform well at the laboratory scale remain difficult to manufacture reliably at the industrial scale. One reason for this disconnect is that biomaterial structure and function are strongly influenced by the physical conditions present during assembly, including flow, transport, dewatering, and drying conditions that differ between laboratory and industrial environments. Here, I propose that scalable biomaterials should be designed with formation conditions and manufacturing environments considered from the outset instead of introducing process constraints only after laboratory optimization. Using papermaking and related large-scale bio-based manufacturing systems as examples, this perspective examines how process-dependent assembly governs interfacial structure development across systems including microfibrillated cellulose, protein-based materials, biofilms, and mycelium composites. Differences between benchtop and industrial processing are discussed in terms of flow behavior, transport limitations, shear history, and consolidation dynamics, demonstrating that scale-dependent physical regimes can produce fundamentally different structures and functional behavior. Based on these observations, a process-integrated framework for biomaterials design is proposed in which material selection, structure formation, and manufacturing constraints are treated as interconnected design variables. The perspective also outlines future needs in process-relevant characterization, multiscale modeling, and experimental tools capable of probing biomaterial formation under realistic manufacturing conditions. Incorporating formation pathways into biomaterials research may improve reproducibility and help bridge the gap between laboratory discovery and industrial implementation.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-synthesized Mg-doped CuO nanoparticles targeting septicemia pathogens: Enhanced antibacterial and cancer cell cytotoxicity.","authors":"Murugeswari Raju, M Karunakaran, M Divya Gnaneswari, P Shunmuga Sundaram, D Shanmugapriya, L Bruno Chandrasekar","doi":"10.1116/6.0005450","DOIUrl":"https://doi.org/10.1116/6.0005450","url":null,"abstract":"<p><p>Copper oxide (CuO) and Mg-doped CuO (CuO-Mg) nanoparticles were synthesized using a green approach with Azadirachta indica leaf extract. The biosynthesized nanoparticles were characterized using multiple techniques including x-ray diffraction (XRD), energy-dispersive x-ray (EDX) analysis, scanning electron microscopy (SEM), and UV-Vis-NIR spectrometry. The XRD analysis verified the purity of the synthesized materials and confirmed a monoclinic crystal structure. Doping CuO with Mg decreased the average crystallite size. EDX spectral analysis confirmed the presence of Cu, Mg, and O elements in the Mg-doped nanoparticles. The optical bandgap ranged from 1.28 to 1.37 eV, in which undoped CuO exhibited a high optical bandgap. The antibacterial activity of CuO nanoparticles increased upon increasing the concentration of Mg doping against the tested pathogens, including Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. The biosynthesized CuO and Mg-doped CuO nanoparticles demonstrated potent concentration-dependent cytotoxic effects on breast cancer cells, achieving complete growth inhibition at 70 μg/ml CuO and 60 μg/ml Mg-doped CuO, with IC50 values of approximately 35.4 and 30.0 μg/ml, respectively.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From structural mimicry to functional maturation: Advances in functional vascular 3D bioprinting.","authors":"Tian Jiao, Chaofan Sun, Congzheng Zhang, Liang Li","doi":"10.1116/6.0005743","DOIUrl":"https://doi.org/10.1116/6.0005743","url":null,"abstract":"<p><p>Vascularization is a core bottleneck restricting the clinical translation of 3D bioprinted tissues and organs. Current vascular bioprinting is undergoing a pivotal shift from simple structural mimicry to functional bionic reconstruction, yet structurally qualified printed vessels often fail in long-term in vivo patency and physiological function due to insufficient biological activity. This review systematically summarizes the recent research progress of functional vascular bioprinting, focusing on advanced functional biomaterials, cutting-edge printing technologies, and core functional optimization strategies. We first clarify the essential differences between structural printing and functional vascular construction and establish a standardized functional evaluation system for bioprinted vessels. This paper further elaborates on the design and optimization of smart composite bioinks; highlights emerging high-fidelity printing technologies including embedded suspension printing, field-assisted microscale printing, and in situ printing; and summarizes key strategies for vascular functional maturation, covering endothelial barrier reconstruction, antithrombotic/anti-inflammatory modification, mechanical domestication, and in vivo remodeling. We also discuss the latest biomedical applications, translational bottlenecks, and future development directions of intelligent biomaterials, multi-scale printing, and standardized translational systems. This review provides a systematic reference for promoting the transformation of vascular bioprinting from structural fabrication to clinical functional application, advancing the development of vascular regenerative medicine.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From protein corona characterization to computational and AI-enabled approaches: Mapping the evolution of nano-bio interface research.","authors":"Hongdong Hao, Huixi Bo, Dian Chen, Xuanyi Zhou, Cheng Qian, Guanlin Wang","doi":"10.1116/6.0005557","DOIUrl":"https://doi.org/10.1116/6.0005557","url":null,"abstract":"<p><p>Protein corona formation critically shapes nano-bio interactions by influencing nanoparticle biological identity, cellular uptake, biodistribution, toxicity, and performance in nanomedicine. To characterize the evolution of protein corona research and the emergence of computational approaches, we conducted a comparative bibliometric analysis of records retrieved on July 25, 2026, using the Web of Science Core Collection as the primary database and Scopus and PubMed for external validation. Two complementary corpora were constructed: a general protein corona corpus comprising 1212 documents published between 2000 and 2025 and an AI-enabled computational protein corona corpus comprising 310 documents published between 2011 and 2025. Bibliometric analyses included global collaboration, publication sources, keyword co-occurrence, thematic evolution, co-citation, bibliographic coupling, citation bursts, and cross-database consistency. The general corpus showed that protein corona research has evolved from physicochemical characterization and protein adsorption toward biological identity, nanomedicine, drug delivery, and functional interpretation. The computational corpus retained the central concepts, publication sources, and citation foundations of the established field, while machine learning, molecular dynamics, and artificial intelligence emerged as prominent methodological themes. These patterns indicate that computational development combines data-driven prediction with mechanistic simulation rather than forming an isolated AI subfield. Scopus and PubMed validation reproduced the major thematic direction identified in Web of Science, although individual frequencies and network indicators differed across databases. Overall, protein corona research exhibits a two-layer structure consisting of an established biomedical nano-bio interface field and an emerging AI-enabled computational extension, reflecting a gradual transition from descriptive characterization toward computationally assisted and prediction-oriented investigation.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of green-synthesized SiO2 nanoparticles in modulating the electrochemical and mechanical properties of poly(vinyl alcohol)/LiClO4 solid polymer electrolytes.","authors":"Satyappa Kalliguddi, R F Bhajantri, Shivaprasad Chalawadi, Suhas M Malenahalli, Mallikarjun Hoogar","doi":"10.1116/6.0005591","DOIUrl":"https://doi.org/10.1116/6.0005591","url":null,"abstract":"<p><p>Poly(vinyl alcohol) (PVA)-based solid polymer electrolytes doped with lithium perchlorate (LiClO4) and reinforced with green-synthesized SiO2 nanoparticles derived from bamboo leaves were prepared by solution casting. Composites containing 0, 0.5, 1.0, and 2.5 wt. % SiO2 in a PVA/20 wt. % LiClO4 matrix were systematically characterized by FTIR, XRD, field emission scanning electron microscopy, TEM, differential scanning calorimetry, thermogravimetric analysis, AC impedance spectroscopy, and universal tensile testing. Structural analyses confirmed successful complexation, increased amorphous nature, and uniform filler dispersion at low loadings, transitioning to agglomeration at higher content. Mechanical testing showed the pristine film exhibited the highest ultimate tensile strength (≈23.5 MPa) and superior ductility. Unexpectedly, the pristine PVA/LiClO4 film exhibited the highest room-temperature ionic conductivity (2.80 × 10-4 S cm-1), which decreased progressively with SiO2 addition. Dielectric, modulus, and relaxation studies revealed that the bamboo-derived SiO2 acted as an insulating barrier, restricting segmental motion and trapping charge carriers rather than facilitating ion transport. This source-specific behavior highlights the critical influence of nanoparticle surface chemistry on electrolyte performance, establishing the unfilled PVA/LiClO4 composition as the optimal system among those investigated for potential applications in solid-state lithium batteries.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical studies of nonconventional drug interaction with human invariant natural killer T cells: Prediction from molecular simulations.","authors":"O A Hernández-Jaimes, J M Zárate-Reyes, M A López-Luis, E Ortiz-Sánchez, S Gallardo-Hernández","doi":"10.1116/6.0005224","DOIUrl":"https://doi.org/10.1116/6.0005224","url":null,"abstract":"<p><p>This study examines sulfamethoxazole (SMX), a sulfonamide antibiotic strongly associated with severe cutaneous adverse effects, as a potential regulator of invariant natural killer T (iNKT) cells. This work combines molecular docking (to evaluate the likelihood of SMX binding to residues within a conventional binding pocket), molecular dynamics (to assess the stability of SMX binding and the forces that may destabilize the interaction), flow cytometry (to evaluate iNKT-cell activation in the presence of SMX within the context of established antigen presentation models), and particularly Raman spectroscopy (to investigate whether SMX-associated spectral features are retained after co-incubation and interaction) directly on sorted iNKT cells. Functional activation was assessed through IFN-γ production from sorted iNKT cells and correlated with Raman spectral data to support our hypothesis. The Raman spectroscopy was performed on both CD1d dimer (in isolation) as the presenter molecule of the drug and in sorted iNKT cells (in isolation), to identify spectroscopic evidence of drug-CD1dimer or drug-cell interactions under near-physiological conditions. From the CD1d experiment, we observed spectral features consistent with potential interactions between CD1d and serum-derived lipids, in a manner comparable to the positive control (α-GalCer). From the iNKT experiment, peaks at 1610 and 1663 cm-1 are the representative peaks that emerge under SMX stimulation conditions in iNKT cells (through TCR binding), as identified by second principal component (PC2) analysis. From the complete experiment, (CD1d + iNKT) suggests the possibility of a direct interaction between SMX and iNKT TCRs. Flow cytometry analysis further demonstrated a significant increase in ZAP-70 phosphorylation in sorted iNKT cells exposed to SMX under serum-free conditions, supporting the possibility of proximal TCR-associated signaling in the absence of CD1d.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vanadium in the radular teeth of the Antarctic Aegires albus (Nudibranchia).","authors":"Wencke Krings, Levin Uhlmann, Heike Wägele, Stanislav N Gorb","doi":"10.1116/6.0005547","DOIUrl":"10.1116/6.0005547","url":null,"abstract":"<p><p>Radular teeth are increasingly recognized as functionally graded composite structures, yet such structure-property relationships remain poorly understood, especially in species from remote habitats. Here, we investigated the radular teeth of the Antarctic sponge-feeding nudibranch Aegires albus using scanning electron microscopy, confocal laser scanning microscopy, energy-dispersive x-ray spectroscopy, and nanoindentation. We compared the elemental composition of tissues, tooth coating, and inner tooth structure and analyzed regional mechanical gradients within the coating. Both the tooth coating and the inner structure contained calcium and vanadium, whereas these elements were much less abundant in the nonradular tissues. Within the coating, both elements showed clear regional gradients. The coating also exhibited pronounced mechanical gradients: Young's modulus and hardness were lowest in the basis and plate, intermediate in the inner structure, and highest in the stylus and tip. Both calcium and vanadium were positively associated with the mechanical properties in all regions of the teeth. These results show that the radular teeth of A. albus are spatially differentiated, multimaterial structures likely adapted to repeated interaction with spicule-bearing sponge prey. In addition, this study documents substantial amounts of vanadium in radular teeth for the first time.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148599024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fiber-reinforced hydrogels: From multiscale structural design to advanced engineering applications.","authors":"Bingtao Li, Anzhu Peng, Xinting Dong, Yujie Gao, Jindan Wu","doi":"10.1116/6.0005586","DOIUrl":"10.1116/6.0005586","url":null,"abstract":"<p><p>Hydrogels are high-water-content polymer networks similar to those of soft tissues and have shown immense potential in fields such as tissue engineering, flexible electronics, and intelligent sensors. However, traditional hydrogels still face challenges such as low mechanical strength, poor toughness, and susceptibility to fatigue. Inspired by tough natural soft tissues (such as muscles and tendons), the introduction of a robust fibrous network into hydrogels enables effective stress transfer, crack bridging, and energy dissipation, thus overcoming the mechanical limitations of traditional hydrogels. This paper reviews fiber-reinforced hydrogels prepared from different reinforcing fibers (e.g., natural, synthetic, inorganic, and carbon-based), as well as the interfacial interactions between the fibers and the matrix (including physical entanglement, dynamic noncovalent bonds, and covalent bonds), and summarizes the preparation methods, such as in situ infiltration, directional freezing, and 3D printing. It also discusses their applications in the fields of medicine, sensing, and wearable devices and finally provides an outlook on current challenges such as precise interface regulation and large-scale intelligent manufacturing.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"21 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}