Biointerphases最新文献

Dissecting the role of substrate folding in enzymatic digestion. 剖析底物折叠在酶消化中的作用。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004803

Nilimesh Das, Tanmoy Khan, Soumya Chaudhury, Bhaswati Sengupta, Pratik Sen

{"title":"Dissecting the role of substrate folding in enzymatic digestion.","authors":"Nilimesh Das, Tanmoy Khan, Soumya Chaudhury, Bhaswati Sengupta, Pratik Sen","doi":"10.1116/6.0004803","DOIUrl":"10.1116/6.0004803","url":null,"abstract":"The efficiency of enzymatic proteolysis is often attributed to the properties of the enzyme itself, with the substrate typically viewed as a passive participant. In this study, we demonstrate that the conformational state of the substrate critically influences proteolytic efficiency. Using human serum albumin (HSA) as a model substrate, papain as the enzyme, and urea as a controlled denaturing agent, we systematically investigated how substrate conformation might affect proteolysis. While papain maintains its structural and functional integrity across varying urea concentrations, HSA transitions through well-defined conformational states (native, compact intermediate, and unfolded), allowing us an opportunity to isolate the effects of the substrate structure. Utilizing site-specific fluorescent labeling and single-molecule fluorescence correlation spectroscopy, we monitor the progression of proteolysis. Our results show that digestion slows at 3M urea, where HSA adopts a compact form, and accelerates at 6M, where HSA takes on an unfolded state, compared to native HSA. These results reveal that substrate folding critically influences the digestion kinetics, probably by controlling protease accessibility and underscoring its importance in mechanistic enzymology and proteomics workflows.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding a plasma copolymerization fit that is "just right" using pentane and acrylic acid precursors. 使用戊烷和丙烯酸前体寻找“刚刚好”的等离子体共聚适合度。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004873

Alexzandria Ledezma, Mollie Corbett, Bethany Yashkus, Mackenzie Jackson, Kristina D Closser, Joshua M Blechle, Morgan J Hawker

{"title":"Finding a plasma copolymerization fit that is \"just right\" using pentane and acrylic acid precursors.","authors":"Alexzandria Ledezma, Mollie Corbett, Bethany Yashkus, Mackenzie Jackson, Kristina D Closser, Joshua M Blechle, Morgan J Hawker","doi":"10.1116/6.0004873","DOIUrl":"https://doi.org/10.1116/6.0004873","url":null,"abstract":"Plasma-enhanced chemical vapor deposition is a versatile technology to control interactions at the biomaterial/biological environment interface. Plasma copolymerization is a related strategy that utilizes a mixed feedgas of two or more plasma precursors, whereby conformal coating surface properties can be controlled by simply varying the feedgas composition. This study reports a previously unexplored combination of plasma precursors-pentane and acrylic acid-to deposit coatings with tunable chemistry and wettability on silk fibroin constructs. Five pentane/acrylic acid feedgas compositions were utilized, ranging from 100%, 75%, 50%, 25%, to 0% pentane by pressure. Plasma-deposited coating properties were evaluated through water contact angle goniometry and x-ray photoelectron spectroscopy. Coating static water contact angle values were tunable between >90° and <55° depending on the feedgas composition. Plasma diagnostics and density functional theory were used to evaluate plasma precursor fragmentation. This library of plasma-modified silk-based materials can be used to design biomaterial surfaces that are \"just right\" for the intended biomedical setting.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimization of decellularized seaweed scaffolds for tissue engineering. 组织工程用脱细胞海藻支架的研制与优化。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004685

Gobinath Chithiravelu, Marion J Jones, Ivana Hernandez de Estrada, Yadvendra Singh, Harish Subbaraman, Binata Joddar

{"title":"Development and optimization of decellularized seaweed scaffolds for tissue engineering.","authors":"Gobinath Chithiravelu, Marion J Jones, Ivana Hernandez de Estrada, Yadvendra Singh, Harish Subbaraman, Binata Joddar","doi":"10.1116/6.0004685","DOIUrl":"10.1116/6.0004685","url":null,"abstract":"In this study, the marine red seaweed Devaleraea mollis (commonly known as Pacific dulse) was investigated as a green, sustainable, and animal-free tissue scaffold alternative, owing to its extracellular matrix mimicking properties. A decellularization-recellularization approach was employed to develop cellulose-based scaffolds capable of supporting human cardiomyocyte growth. Native dulse samples were cleaned, dried, and decellularized using varying concentrations of sodium dodecyl sulfate (SDS) (3%, 5%, 7%, 10%, 12%, and 15%), with Triton X-100 (2%) and NaClO (0.2%). The resulting scaffolds were comprehensively characterized using light microscopy, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy, and Raman spectroscopy to identify the conditions that best preserved the fibrous, honeycombed architecture and cellulose-rich content of the native tissue scaffold. Among all treatments, scaffolds processed with 10%, 12%, and 15% SDS exhibited superior structural integrity and biochemical preservation, emerging as the most effective formulations. These selected scaffolds were then subjected to swelling analysis to evaluate biodegradation behavior, followed by in vitro cell culture to assess biocompatibility. All tested scaffolds demonstrated excellent compatibility with human cardiomyocytes, maintaining high cell viability and proliferation for one week of in vitro culture, as confirmed by SEM and immunohistochemistry. Notably, a 90% scaffold surface coverage by cardiac cells on day 6, accompanied by a 2.5 times normalized cell proliferation, indicated robust cell attachment and proliferation. Collectively, these findings highlight seaweed-derived cellulose as a highly promising, biocompatible, and eco-friendly biomaterial, posing itself as a novel interface for diverse biomedical applications and innovations in sustainable tissue engineering.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium is involved in protein cohesion and interfacial adhesion in a marine invasive fouling ascidian. 钙参与海洋侵入性污垢海鞘的蛋白质内聚和界面粘附。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004889

Shiguo Li, Ying Zhang, Dan He, Miaolian Zhang, Aibin Zhan

{"title":"Calcium is involved in protein cohesion and interfacial adhesion in a marine invasive fouling ascidian.","authors":"Shiguo Li, Ying Zhang, Dan He, Miaolian Zhang, Aibin Zhan","doi":"10.1116/6.0004889","DOIUrl":"https://doi.org/10.1116/6.0004889","url":null,"abstract":"Protein-mediated underwater adhesion is vital for the survival of many aquatic organisms and plays central roles in biofouling and bioinspired material development. Metal ions are known to influence underwater adhesion by regulating cohesion between adhesive proteins and interactions at the underwater material interface. However, direct mechanistic evidence of Ca2+ involvement in adhesion of marine organisms remains insufficient. In this study, we investigated the role of Ca2+ in permanent underwater adhesion of ascidian adhesive protein 1 (AAP1), an adhesive protein identified from the ascidian Ciona robusta, a model marine invasive fouling species. Using in vitro experiments, we examined AAP1's cohesion and interfacial adhesion under varying Ca2+ concentrations (0, 1.0, 2.5, 5.0, 10.0, and 25.0 mM). Our results indicated that Ca2+ mediated both cohesion and interfacial adhesion in a concentration-dependent manner. Protein aggregation was induced at 10.0 and 25.0 mM, with denser aggregation at higher concentrations. Surface force apparatus measurements showed a peak in cohesion energy at 25.0 mM Ca2+, while interfacial adhesion energy reached a maximum at 10.0 mM. These results suggest that Ca2+ may facilitate cohesion via salt bridge formation and promote interfacial adhesion by mediating electrostatic interactions between AAP1 and material surfaces. Additionally, the cohesion of AAP1 may enhance molecular alignment on surfaces, contributing its interfacial adhesion. Overall, our results provide direct evidence for the involvement of Ca2+ in protein-mediated ascidian underwater adhesion. These findings will deepen our understanding of the mechanisms of underwater adhesion in aquatic organisms and guide the future development of antifouling strategies and bioinspired underwater adhesives.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico self-assembly and complexation dynamics of cationic lipids with DNA nanocages to enhance lipofection. 阳离子脂质与DNA纳米笼的硅自组装和络合动力学以增强脂质感染。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004756

Sandip Mandal, Dhiraj Bhatia, Prabal K Maiti

{"title":"In silico self-assembly and complexation dynamics of cationic lipids with DNA nanocages to enhance lipofection.","authors":"Sandip Mandal, Dhiraj Bhatia, Prabal K Maiti","doi":"10.1116/6.0004756","DOIUrl":"https://doi.org/10.1116/6.0004756","url":null,"abstract":"DNA nanostructures are promising materials for drug delivery due to their unique topology, shape, size control, biocompatibility, structural stability, and blood-brain-barrier penetration capability. However, their cellular permeability is hindered by strong electrostatic repulsion from negatively charged cellular membranes, posing a significant obstacle to the use of DNA nanostructures as a drug delivery vehicle. Recent experimental studies have shown enhanced cellular uptake for the conjugate binary mixtures of DNA Tetrahedron (TDN) with cationic lipid N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) compared to TDN alone. However, the cationic DOTMA lipid binding mechanism with the TDN nucleotides is still elusive. Using fully atomistic MD simulations, we aim to understand the molecular interactions that drive the formation and stability of the TDN-DOTMA binary complexes in a physiological environment. Our results uncovered that lipid concentration plays a crucial role in the energetics of the TDN-DOTMA association. We also report that distinct time scales are associated with the self-assembly of cationic DOTMA lipids first, followed by the complexation of self-assembled DOTMA lipid clusters with the TDN nucleotides, where electrostatics, hydrophobicity, and hydrogen bonding are the key interactions that drive the formation and stability of these complexes. Our results provide molecular insights into TDN-DOTMA interactions, highlighting the lipid self-assembly dynamics, complex stability, and morphology, paving the way for the better rational design of cationic lipid-functionalized DNA nanostructures for efficient drug delivery and transfection.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human osteoblast derived biochemical cues and microsurface topography modulate osteogenesis in vitro and in vivo. 人成骨细胞衍生的生化线索和微表面地形在体外和体内调节成骨。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-09-01 DOI: 10.1116/6.0004679

Deepa Mishra, Anne Bernhardt, Michael Gelinsky, Bikramjit Basu

{"title":"Human osteoblast derived biochemical cues and microsurface topography modulate osteogenesis in vitro and in vivo.","authors":"Deepa Mishra, Anne Bernhardt, Michael Gelinsky, Bikramjit Basu","doi":"10.1116/6.0004679","DOIUrl":"https://doi.org/10.1116/6.0004679","url":null,"abstract":"Indirect co-culture, wherein two distinct cell types are cultivated within the same medium without direct contact, remains a relatively underexplored approach in biomaterials science for simulating physiological cell-cell interactions on material surfaces in vitro. In this study, human mesenchymal stem cells (hMSCs) were cultured on two types of Ti6Al4V substrates (polished and sand-blasted/acid etched) in a co-culture system using conditioned osteogenic differentiation media (cOBM), enriched with soluble factors secreted by human osteoblasts (hOBs). The combined impact of surface microtopography of Ti6Al4V substrates and cOBM supplementation has resulted in the modulation of cell morphology, alkaline phosphatase (ALP) activity, and calcium phosphate mineralization. Enhanced mineralization (2.5-fold increase compared to baseline at day 21) was observed on Ti6Al4V substrates when hMSCs were cultured in the presence of cOBM. This was accompanied by a peak expression of the early osteogenic marker, ALP by day 14. The synergistic behavior of sandblasted and acid-etched substrates with soluble biochemical cues, derived from hOBs showcased their potential for augmenting osteogenic differentiation. The in vitro outcomes were validated in a rabbit model study, which clearly demonstrated better osseointegration of sand-blasted/acid etched implants over 12 weeks.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical methods to study the complex dynamics of biofilm-biomaterial interfaces. 研究生物膜-生物材料界面复杂动力学的分析方法。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004613

Yunxing Li, Dipankar Koley

{"title":"Analytical methods to study the complex dynamics of biofilm-biomaterial interfaces.","authors":"Yunxing Li, Dipankar Koley","doi":"10.1116/6.0004613","DOIUrl":"https://doi.org/10.1116/6.0004613","url":null,"abstract":"Biofilm-biomaterial interfaces have an important role in biofilm development and pose a critical challenge in healthcare, contributing to device failures and chronic infections that affect patient outcomes and healthcare economics. This review explores the complex dynamics of these interfaces, from initial protein adsorption through mature biofilm development, highlighting how bacteria and materials are involved in bidirectional interactions that determine both infection progression and material degradation. It also examines different advanced analytical methods for characterizing these dynamic biofilm-biomaterial interactions, with particular emphasis on the recent developments in electrochemical techniques (ion-selective electrodes, electrochemical impedance spectroscopy, and scanning electrochemical microscopy) that enable real-time monitoring of critical parameters such as pH, oxygen gradients, and metabolic activities, providing unique insights into biofilm heterogeneity and localized chemical changes. In addition, the review explores future developments in sensor technology and standardized protocols needed to accelerate biomaterial innovation, potentially transforming our approach to implant-associated infections through responsive surfaces that adapt to microbial challenges.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zeolite substrate characterization for Metarhizium robertsii inoculation. 罗伯特绿僵菌接种的沸石基质表征。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004518

Ángel Guillén-Cervantes, Francisco Hernández-Rosas, Blas Schettino-Salomón, José Alberto Aguilar-Ayala, Felipe Francisco Muñoz-Ponce, Juan Hernández-Rosas

{"title":"Zeolite substrate characterization for Metarhizium robertsii inoculation.","authors":"Ángel Guillén-Cervantes, Francisco Hernández-Rosas, Blas Schettino-Salomón, José Alberto Aguilar-Ayala, Felipe Francisco Muñoz-Ponce, Juan Hernández-Rosas","doi":"10.1116/6.0004518","DOIUrl":"https://doi.org/10.1116/6.0004518","url":null,"abstract":"For this study, zeolite powder served as a substrate for inoculating Metarhizium robertsii to demonstrate the biocompatibility between the entomopathogenic fungus and the zeolite mineral, as the initial step in developing a biological control agent. Our fungal strains were isolated from corpses of spittlebugs (Aeneolamia albofasciata, Hemiptera: Cercopidae) and were identified as M. robertsii based on sequencing of the Internal Transcribed Spacer regions ITS1 and ITS2. Zeolite was characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). XRD and EDS results indicate that zeolite consists of a mixture of Heulandite and Clinoptilolite. EDS analysis shows that oxygen, silicon, and aluminum are the primary chemical components of the zeolite powder, with calcium, magnesium, iron, sodium, and potassium present in smaller amounts. After five days of inoculation, SEM images reveal M. robertsii conidia on the porous surface of zeolite particles, along with hyphal formation. These findings suggest the potential for maintaining M. robertsii spores and mycelium alive within a zeolite substrate under laboratory conditions.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of vacuum exposure on sample stability for mass spectrometry analysis. 真空暴露对质谱分析样品稳定性的影响。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004632

Matija Lagator, Siyu Liu, C Logan Mackay, Felicia Green

{"title":"Effects of vacuum exposure on sample stability for mass spectrometry analysis.","authors":"Matija Lagator, Siyu Liu, C Logan Mackay, Felicia Green","doi":"10.1116/6.0004632","DOIUrl":"https://doi.org/10.1116/6.0004632","url":null,"abstract":"Mass spectrometry (MS) often requires vacuum conditions, which, while beneficial for analysis, can unpredictably alter sensitive samples. This study investigates the impact of prolonged vacuum exposure on the consistency and reliability of MS detection of thin films of acetaminophen using secondary ion mass spectrometry (SIMS). Under vacuum at room temperature, the mass spectrometry signal intensity decreased by approximately 81.5% over the duration of the experiment (42 h). Optical microscopy revealed that this decrease coincided with sublimation-induced sample loss of the acetaminophen. As a result, acetaminophen coverage across the substrate became heterogeneous, leading to increased relative standard deviation (RSD) in the SIMS signal over time. In contrast, under cryogenic conditions, neither signal degradation nor an increase in RSD was observed. Additionally, a comparison with atmospheric pressure mass spectrometry revealed that, in the absence of vacuum, signal intensity remained more stable over time. These findings highlight the potential drawbacks of vacuum exposure for volatile standards and emphasize the importance of testing vacuum effects prior to analysis. If vacuum is necessary, cryogenic conditions should be considered to mitigate sample degradation. While these effects were observed for a mass spectrometry technique, they are also applicable to any type of vacuum-based methodology where the samples might be prone to sublimation.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced collagenogenesis on three-dimensionally printed titanium surfaces by human gingival fibroblasts: An in vitro study. 人牙龈成纤维细胞增强三维打印钛表面胶原生成的体外研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004500

Vitor de Toledo Stuani, Isabela Sanches Pompeo da Silva, Gustavo Gonçalves do Prado Manfredi, Fernanda Balestrero Cassiano, Larissa Alamo, Ligia Espoliar Corrêa, Jamil Awad Shibli, Carlos Alberto de Souza Costa, Diana Gabriela Soares

{"title":"Enhanced collagenogenesis on three-dimensionally printed titanium surfaces by human gingival fibroblasts: An in vitro study.","authors":"Vitor de Toledo Stuani, Isabela Sanches Pompeo da Silva, Gustavo Gonçalves do Prado Manfredi, Fernanda Balestrero Cassiano, Larissa Alamo, Ligia Espoliar Corrêa, Jamil Awad Shibli, Carlos Alberto de Souza Costa, Diana Gabriela Soares","doi":"10.1116/6.0004500","DOIUrl":"https://doi.org/10.1116/6.0004500","url":null,"abstract":"The lack of cementum in peri-implant tissues leads to a deficiency in anchorage points for gingival collagen fibers. This arrangement is linked to reduced protective capabilities compared to teeth. Therefore, there is a pressing need to develop surfaces that optimize the interaction between soft tissue and implants. 3D-printed titanium disks (Ti3DP), machined disks (TiMC), and glass coverslips (GS) were seeded with human gingival fibroblasts. These specimens underwent mechanical characterization via roughness and wettability assays. Biological characterization included assessments of cellular viability (live/dead), adhesion and spreading (F-actin), cell count (DAPI), cellular metabolism (Alamar blue), adhesive strength, and soluble collagen and total protein quantification up to 14 days. Data analysis employed Student's t-test and ANOVA post-hoc Tukey test (α = 0.05). The group TiMC exhibited higher hydrophilicity and lower roughness compared to Ti3DP. All groups demonstrated cellular viability throughout the study period. Adhesive strength did not significantly differ among groups; however, cell count was higher in TiMC and GS after one day of cell seeding in comparison to Ti3DP. Morphologically, GS and TiMC displayed more fusiform cells with a uniform distribution, while Ti3DP showed smaller, irregular cells with multiple lamellipodia and filopodia. Additionally, statistically superior collagen and total protein deposition was observed in Ti3DP (p < 0.01). The 3D-printed titanium surface allowed human gingival fibroblasts to adhere to it, leading to a 3D cytoskeleton morphology that culminated in increased collagen expression. Therefore, these 3D-printed devices present a promising avenue for producing transmucosal components due to their increase in collagen production.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0