Biointerphases最新文献_第10页

Antimicrobial efficacy of cyclic α- and β-peptides incorporated in polyurethane coatings. 聚氨酯涂料中环α-和β-肽的抗菌效果。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002515

Qin Lu, Daniel P Regan, Daniel E Barlow, Kenan P Fears

{"title":"Antimicrobial efficacy of cyclic α- and β-peptides incorporated in polyurethane coatings.","authors":"Qin Lu, Daniel P Regan, Daniel E Barlow, Kenan P Fears","doi":"10.1116/6.0002515","DOIUrl":"10.1116/6.0002515","url":null,"abstract":"Microbial growth on surfaces poses health concerns and can accelerate the biodegradation of engineered materials and coatings. Cyclic peptides are promising agents to combat biofouling because they are more resistant to enzymatic degradation than their linear counterparts. They can also be designed to interact with extracellular targets and intracellular targets and/or self-assemble into transmembrane pores. Here, we determine the antimicrobial efficacy of two pore-forming cyclic peptides, α-K3W3 and β-K3W3, against bacterial and fungal liquid cultures and their capacity to inhibit biofilm formation on coated surfaces. These peptides display identical sequences, but the additional methylene group in the peptide backbone of β-amino acids results in a larger diameter and an enhancement in the dipole moment. In liquid cultures, β-K3W3 exhibited lower minimum inhibitory concentration values and greater microbicidal power in reducing the number of colony forming units (CFUs) when exposed to a gram-positive bacterium, Staphylococcus aureus, and two fungal strains, Naganishia albida and Papiliotrema laurentii. To evaluate the efficacy against the formation of fungal biofilms on painted surfaces, cyclic peptides were incorporated into polyester-based thermoplastic polyurethane. The formation of N. albida and P. laurentii microcolonies (105 per inoculation) for cells extracted from coatings containing either peptide could not be detected after a 7-day exposure. Moreover, very few CFUs (∼5) formed after 35 days of repeated depositions of freshly cultured P. laurentii every 7 days. In contrast, the number of CFUs for cells extracted from the coating without cyclic peptides was >8 log CFU.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9611922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative evaluation of perfluorinated alkanethiol molecular order on gold surfaces. 金表面全氟烷硫醇分子秩序的定量评估。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002720

Lara J Gamble, David Radford, David W Grainger, David G Castner

{"title":"Quantitative evaluation of perfluorinated alkanethiol molecular order on gold surfaces.","authors":"Lara J Gamble, David Radford, David W Grainger, David G Castner","doi":"10.1116/6.0002720","DOIUrl":"10.1116/6.0002720","url":null,"abstract":"Self-assembled monolayers (SAMs) of perfluoroalkanethiols [CF3(CF2)xCH2CH2SH (x = 3, 5, 7, and 9)] on gold were characterized by x-ray photoelectron spectroscopy (XPS), near edge x-ray absorption fine structure (NEXAFS), and static time-of-flight secondary ion mass spectrometry (ToF-SIMS). Perfluoroalkanethiols of several chain lengths were synthesized using a known hydride reduction method for transforming commercially available perfluoroalkyliodides to corresponding perfluoroalkanethiols. This strategy provides improved product yields compared to other known routes based on hydrolysis from the common thioacetyl perfluoroalkyl intermediate. Angle-dependent XPS analysis revealed that CF3(CF2)xCH2CH2SH (x = 5, 7, and 9; F6, F8, and F10, respectively) SAMs on gold exhibited significant enrichment of the terminal CF3 group at the outer monolayer surface with the sulfur present as a metal-bound thiolate located at the monolayer-gold interface. XPS of the CF3(CF2)3CH2CH2SH (F4) monolayer revealed a thin film with a significant (>50%) amount of hydrocarbon contamination consistent with poorly organized monolayers, while the longest thiol (F10) showed XPS signals attributed to substantial ordering and anisotropy. ToF-SIMS spectra from all four SAMs contained molecular ions representative of the particular perfluorinated thiol used to prepare the monolayer. NEXAFS methods were used to determine degrees of ordering and average tilt for molecules comprising monolayers. The SAMs prepared from the longest (F10) thiols exhibited the highest degree of ordering with the molecular axis nearly perpendicular to the gold surface. The degree of ordering decreased significantly with decreasing length of the perfluorocarbon tail.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9638866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interdependence of cholesterol distribution and conformational order in lipid bilayers. 脂质双分子层中胆固醇分布和构象顺序的相互依赖性。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002489

Mohammadreza Aghaaminiha, Amir M Farnoud, Sumit Sharma

{"title":"Interdependence of cholesterol distribution and conformational order in lipid bilayers.","authors":"Mohammadreza Aghaaminiha, Amir M Farnoud, Sumit Sharma","doi":"10.1116/6.0002489","DOIUrl":"https://doi.org/10.1116/6.0002489","url":null,"abstract":"We show, via molecular simulations, that not only does cholesterol induce a lipid order, but the lipid order also enhances cholesterol localization within the lipid leaflets. Therefore, there is a strong interdependence between these two phenomena. In the ordered phase, cholesterol molecules are predominantly present in the bilayer leaflets and orient themselves parallel to the bilayer normal. In the disordered phase, cholesterol molecules are mainly present near the center of the bilayer at the midplane region and are oriented orthogonal to the bilayer normal. At the melting temperature of the lipid bilayers, cholesterol concentration in the leaflets and the bilayer midplane is equal. This result suggests that the localization of cholesterol in the lipid bilayers is mainly dictated by the degree of ordering of the lipid bilayer. We validate our findings on 18 different lipid bilayer systems, obtained from three different phospholipid bilayers with varying concentrations of cholesterol. To cover a large temperature range in simulations, we employ the Dry Martini force field. We demonstrate that the Dry and the Wet Martini (with polarizable water) force fields produce comparable results.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9521287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facilitated osteogenesis of magnesium implant by coating of strontium incorporated calcium phosphate. 磷酸钙锶包覆促进镁种植体成骨。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002598

Jung-Eun Park, Yong-Seok Jang, Jae-Min Seo, Min-Ho Lee

引用次数: 0

Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS. 生物膜中铜绿假单胞菌胞外多糖Psl的3DOrbiSIMS鉴定。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002604

Heba Khateb, Andrew L Hook, Stefanie Kern, Julie A Watts, Sonali Singh, Darryl Jackson, Luisa Marinez-Pomares, Paul Williams, Morgan R Alexander

{"title":"Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS.","authors":"Heba Khateb, Andrew L Hook, Stefanie Kern, Julie A Watts, Sonali Singh, Darryl Jackson, Luisa Marinez-Pomares, Paul Williams, Morgan R Alexander","doi":"10.1116/6.0002604","DOIUrl":"10.1116/6.0002604","url":null,"abstract":"Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preserve the 3D location of an analyte in a sample. Polysaccharides are recognized as challenging analytes in the mass spectrometry of liquids and are also difficult to identify and assign using SIMS. Psl is an exopolysaccharide produced by Pseudomonas aeruginosa, which plays a key role in biofilm formation and maturation. In this Letter, we describe the use of the OrbiTrap analyzer with SIMS (3D OrbiSIMS) for the label-free mass spectrometry of Psl, taking advantage of its high mass resolving power for accurate secondary ion assignment. We study a P. aeruginosa biofilm and compare it with purified Psl to enable the assignment of secondary ions specific to the Psl structure. This resulted in the identification of 17 peaks that could confidently be ascribed to Psl fragments within the biofilm matrix. The complementary approach of the following neutral loss sequences is also shown to identify multiple oligosaccharide fragments without the requirement of a biological reference sample.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the assembly of lipid-hyaluronan complexes in osteoarthritic conditions. 洞察脂质-透明质酸复合物在骨关节炎条件下的组装。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-04-11 DOI: 10.1116/6.0002502

Kangdi Sun, Tooba Shoaib, Mark W Rutland, Joseph Beller, Changwoo Do, Rosa M Espinosa-Marzal

{"title":"Insight into the assembly of lipid-hyaluronan complexes in osteoarthritic conditions.","authors":"Kangdi Sun, Tooba Shoaib, Mark W Rutland, Joseph Beller, Changwoo Do, Rosa M Espinosa-Marzal","doi":"10.1116/6.0002502","DOIUrl":"https://doi.org/10.1116/6.0002502","url":null,"abstract":"Interactions between molecules in the synovial fluid and the cartilage surface may play a vital role in the formation of adsorbed films that contribute to the low friction of cartilage boundary lubrication. Osteoarthritis (OA) is the most common degenerative joint disease. Previous studies have shown that in OA-diseased joints, hyaluronan (HA) not only breaks down resulting in a much lower molecular weight (MW), but also its concentration is reduced ten times. Here, we have investigated the structural changes of lipid-HA complexes as a function of HA concentration and MW to simulate the physiologically relevant conditions that exist in healthy and diseased joints. Small angle neutron scattering and dynamic light scattering were used to determine the structure of HA-lipid vesicles in bulk solution, while a combination of atomic force microscopy and quartz crystal microbalance was applied to study their assembly on a gold surface. We infer a significant influence of both MW and HA concentrations on the structure of HA-lipid complexes in bulk and assembled on a gold surface. Our results suggest that low MW HA cannot form an amorphous layer on the gold surface, which is expected to negatively impact the mechanical integrity and longevity of the boundary layer and could contribute to the increased wear of the cartilage that has been reported in joints diseased with OA.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 2","pages":"021005"},"PeriodicalIF":2.1,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix. 局部粘附介导的亚细胞曲率传感对工程细胞外基质反应的评估。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-04-05 DOI: 10.1116/6.0002440

Daniel T Bowers, Mary Elizabeth McCulloch, Justin L Brown

{"title":"Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.","authors":"Daniel T Bowers, Mary Elizabeth McCulloch, Justin L Brown","doi":"10.1116/6.0002440","DOIUrl":"10.1116/6.0002440","url":null,"abstract":"Fibril curvature is bioinstructive to attached cells. Similar to natural healthy tissues, an engineered extracellular matrix can be designed to stimulate cells to adopt desired phenotypes. To take full advantage of the curvature control in biomaterial fabrication methodologies, an understanding of the response to fibril subcellular curvature is required. In this work, we examined morphology, signaling, and function of human cells attached to electrospun nanofibers. We controlled curvature across an order of magnitude using nondegradable poly(methyl methacrylate) (PMMA) attached to a stiff substrate with flat PMMA as a control. Focal adhesion length and the distance of maximum intensity from the geographic center of the vinculin positive focal adhesion both peaked at a fiber curvature of 2.5 μm-1 (both ∼2× the flat surface control). Vinculin experienced slightly less tension when attached to nanofiber substrates. Vinculin expression was also more affected by a subcellular curvature than structural proteins α-tubulin or α-actinin. Among the phosphorylation sites we examined (FAK397, 576/577, 925, and Src416), FAK925 exhibited the most dependance on the nanofiber curvature. A RhoA/ROCK dependance of migration velocity across curvatures combined with an observation of cell membrane wrapping around nanofibers suggested a hybrid of migration modes for cells attached to fibers as has been observed in 3D matrices. Careful selection of nanofiber curvature for regenerative engineering scaffolds and substrates used to study cell biology is required to maximize the potential of these techniques for scientific exploration and ultimately improvement of human health.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 2","pages":"021004"},"PeriodicalIF":1.6,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10661787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Back to the basics of time-of-flight secondary ion mass spectrometry of bio-related samples. I. Instrumentation and data collection. 生物相关样品的飞行时间二次离子质谱分析的基本原理。I. 仪器和数据采集。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-29 DOI: 10.1116/6.0002477

Daniel J Graham, Lara J Gamble

引用次数: 0

3D bioprinting of gastrointestinal cancer models: A comprehensive review on processing, properties, and therapeutic implications. 胃肠道肿瘤模型的3D生物打印:加工、特性和治疗意义的综合综述。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-24 DOI: 10.1116/6.0002372

Kalappa Prashantha, Amita Krishnappa, Malini Muthappa

{"title":"3D bioprinting of gastrointestinal cancer models: A comprehensive review on processing, properties, and therapeutic implications.","authors":"Kalappa Prashantha, Amita Krishnappa, Malini Muthappa","doi":"10.1116/6.0002372","DOIUrl":"https://doi.org/10.1116/6.0002372","url":null,"abstract":"Gastrointestinal tract (GIT) malignancies are an important public health problem considering the increased incidence in recent years and the high morbidity and mortality associated with it. GIT malignancies constitute 26% of the global cancer incidence burden and 35% of all cancer-related deaths. Gastrointestinal cancers are complex and heterogenous diseases caused by the interplay of genetic and environmental factors. The tumor microenvironment (TME) of gastrointestinal tract carcinomas is dynamic and complex; it cannot be recapitulated in the basic two-dimensional cell culture systems. In contrast, three-dimensional (3D) in vitro models can mimic the TME more closely, enabling an improved understanding of the microenvironmental cues involved in the various stages of cancer initiation, progression, and metastasis. However, the heterogeneity of the TME is incompletely reproduced in these 3D culture models, as they fail to regulate the orientation and interaction of various cell types in a complex architecture. To emulate the TME, 3D bioprinting has emerged as a useful technique to engineer cancer tissue models. Bioprinted cancer tissue models can potentially recapitulate cancer pathology and increase drug resistance in an organ-mimicking 3D environment. In this review, we describe the 3D bioprinting methods, bioinks, characterization of 3D bioprinted constructs, and their application in developing gastrointestinal tumor models that integrate their microenvironment with different cell types and substrates, as well as bioprinting modalities and their application in therapy and drug screening. We review prominent studies on the 3D bioprinted esophageal, hepatobiliary, and colorectal cancer models. In addition, this review provides a comprehensive understanding of the cancer microenvironment in printed tumor models, highlights current challenges with respect to their clinical translation, and summarizes future perspectives.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 2","pages":"020801"},"PeriodicalIF":2.1,"publicationDate":"2023-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatibility evaluation of polyethersulfone-pyrolytic carbon composite membrane in artificial pancreas. 聚醚砜-热解碳复合膜在人工胰腺中的生物相容性评价。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-21 DOI: 10.1116/6.0002155

Reza Peighami, Mohamadreza Mehrnia, Fatemeh Yazdian, Mojgan Sheikhpour

{"title":"Biocompatibility evaluation of polyethersulfone-pyrolytic carbon composite membrane in artificial pancreas.","authors":"Reza Peighami, Mohamadreza Mehrnia, Fatemeh Yazdian, Mojgan Sheikhpour","doi":"10.1116/6.0002155","DOIUrl":"https://doi.org/10.1116/6.0002155","url":null,"abstract":"Polyethersulfone (PES) membranes are widely used in medical devices, especially intravascular devices such as intravascular bioartificial pancreases. In the current work, the pure PES and PES-pyrolytic carbon (PyC) composite membranes were synthesized and permeability studies were conducted. In addition, the cytocompatibility and hemocompatibility of the pure PES and PES-PyC membranes were investigated. These materials were characterized using peripheral blood mononuclear cell (PBMC) activation, platelet activation, platelet adhesion, ß-cell viability and proliferation, and ß-cell response to hyperglycemia. The results showed that platelet activation decreased from 87.3% to 27.8%. Any alteration in the morphology of sticking platelets was prevented, and the number of attached platelets decreased by modification with PyC. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay corroborated that PBMC activation was encouraged by the PyC-modified PES membrane surface. It can be concluded that PES-modified membranes show higher hemocompatibility than pure PES membranes. ß-cells cultured on all the three membranes displayed a lower rate of proliferation although the cells on the PES-PyC (0.1 wt. %) membrane indicated a slightly higher viability and proliferation than those on the pure PES and PES-PyC (0.05 wt. %) membranes. It shows that the PES-PyC (0.1 wt. %) membrane possesses superior cytocompatibility over the other membranes.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 2","pages":"021003"},"PeriodicalIF":2.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9355620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0