Biointerphases最新文献_第9页

Immobilization of horseradish peroxidase with zwitterionic polymer material for industrial phenolic removal. 两性离子高分子材料固定化辣根过氧化物酶的工业苯酚脱除。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002657

Qi Wang, Hao Fu, Xiaoyu Qi, Lei Zhang, Hongyan Ma

{"title":"Immobilization of horseradish peroxidase with zwitterionic polymer material for industrial phenolic removal.","authors":"Qi Wang, Hao Fu, Xiaoyu Qi, Lei Zhang, Hongyan Ma","doi":"10.1116/6.0002657","DOIUrl":"10.1116/6.0002657","url":null,"abstract":"Horseradish peroxidase (HRP) is a hemoglobin composed of a single peptide chain that catalyzes the oxidation of various substrates such as phenol and aniline in the presence of hydrogen peroxide via its iron-porphyrin catalytic center. This enzyme is widely used in industrial phenol removal, food additives, biomedicine, and clinical test reagents due to its rapid reaction rate and obvious reaction outcomes. However, the large-scale use of HRP in industrial applications still faces numerous challenges, including activity, stability, and sustainability. This study demonstrates that when peroxidase is immobilized in zwitterionic polymer hydrogels, polycarboxybetaine (PCB) and polysulfobetaine (PSB), the properties of the enzyme are improved. PCB and PSB-embedded HRP exhibit a 6.11 and 1.53 times increase in Kcat/Km value, respectively, compared to the free enzyme. The immobilized enzyme also experiences increased activity over a range of temperatures and better tolerance to extreme pH and organic solvents, including formaldehyde. In addition, immobilized HRP exhibits excellent performance in storage and reproducibility. Remarkably, PCB-HRP still retains 80% of the initial activity after a 6-week storage period and can still attain the initial catalytic level of the free enzyme after six repeated cycles. It also removes 90% of phenol within 12 min, surpassing the current pharmacy on the market. These experimental results indicated that we have successfully designed a set of stable and efficient support substrates for horseradish peroxidase, which enhances its suitability for deployment in industrial applications.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9805524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of electronic sum frequency generation spectrophotometer to assess the buried interfaces. 电子和频产生分光光度计的研制。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002697

Suman Dhami, Yogesh Kumar, Ravindra Pandey

{"title":"Development of electronic sum frequency generation spectrophotometer to assess the buried interfaces.","authors":"Suman Dhami, Yogesh Kumar, Ravindra Pandey","doi":"10.1116/6.0002697","DOIUrl":"10.1116/6.0002697","url":null,"abstract":"The interfacial region between two bulk media in organic semiconductor based devices, such as organic field-effect transistors (OFETs), organic light-emitting diodes, and organic photovoltaics, refers to the region where two different materials such as an organic material and an electrode come in contact with each other. Although the interfacial region contains a significantly smaller fraction of molecules compared to the bulk, it is the primary site where many photoinduced excited state processes occur, such as charge transfer, charge recombination, separation, energy transfer processes, etc. All such photoinduced processes have a dependence on molecular orientation and density of states at the interfaces, therefore having an understanding of the interfacial region is essential. However, conventional spectroscopic techniques, such as surface-enhanced Raman scattering, x-ray photoelectron spectroscopy, atomic force microscopy, etc., face limitations in probing the orientation and density of states of interfacial molecules. Therefore, there is a need for noninvasive techniques capable of efficiently investigating the interfaces. The electronic sum frequency generation (ESFG) technique offers an interface selectivity based on the principle that the second-order nonlinear susceptibility tensor, within the electric dipole approximation, is zero in the isotropic bulk but nonzero at interfaces. This selectivity makes ESFG a promising spectroscopy tool to probe the molecular orientation and density of states at the buried interface. For beginners interested in employing ESFG to study the density of states at the interface, a detailed description of the experimental setup is provided here.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9804040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PFAS (per- and polyfluoroalkyl substance)-free molded fiber: The future is already here. 无PFAS(全氟和多氟烷基物质)模制纤维:未来已经到来。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002756

Mehdi Tajvidi

引用次数: 1

Evidence of compost contamination with per- and polyfluoroalkyl substances (PFAS) from "compostable" food serviceware. 来自“可堆肥”食品餐具的全氟和多氟烷基物质(PFAS)污染堆肥的证据。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002746

Caleb P Goossen, Rachel E Schattman, Jean D MacRae

引用次数: 2

Back to the basics of time-of-flight secondary ion mass spectrometry data analysis of bio-related samples. II. Data processing and display. 生物相关样品的飞行时间二次离子质谱数据分析的基本原理。II.数据处理和显示。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002633

Daniel J Graham, Lara J Gamble

引用次数: 0

Molecular simulation studies on a zwitterionic peptide-dendrimer conjugate for integrin αvβ3 binding. 两性离子肽-树状大分子结合整合素αvβ3的分子模拟研究。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002713

Xiaowei Lin, Nan Xu, Chen Li, Zhiyu Wu, Shengfu Chen, Yao Shi, Yi He

{"title":"Molecular simulation studies on a zwitterionic peptide-dendrimer conjugate for integrin αvβ3 binding.","authors":"Xiaowei Lin, Nan Xu, Chen Li, Zhiyu Wu, Shengfu Chen, Yao Shi, Yi He","doi":"10.1116/6.0002713","DOIUrl":"https://doi.org/10.1116/6.0002713","url":null,"abstract":"Zwitterionic dendrimer is an effective carrier, which can restore the natural conformation of peptide segments for high bioaffinity by a hydrogen bond-induced conformational constraint approach. However, it is still unknown whether the approach is applicable for the dendrimers with different geometric sizes. Therefore, the characteristics of conjugates made from zwitterionic poly(amidoamine) (PAM) and the arginine-glycine-aspartic acid (RGD) peptide were examined to elucidate the effects of the geometric sizes of the PAM dendrimer on the conformational structure and stability of the peptide. The results show that the RGD fragments had almost the same structure and stability when conjugated with PAM(G3, G4, or G5) dendrimers. However, when conjugated with PAM(G1 or G2) dendrimers, the structural stability of these fragments was found to be much worse. Also, the structure and stability of RGD segments conjugated with PAM(G3, G4, or G5) were not affected when additional EK segments were inserted. Moreover, we observed that RGD fragments conjugated with PAM(G3, G4, or G5) dendrimers were structurally stable and similar when the concentration of NaCl was 0.15 and 0.5M. Furthermore, we show that PAM(G3, G4, or G5)-RGD conjugates bind strongly to integrin αvβ3.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coarse-grained simulations of lysozyme-silica-nanoparticle corona. 溶菌酶-二氧化硅-纳米粒子电晕的粗粒度模拟。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002736

Lanlan Qin, Gaobo Yu, Jian Zhou

{"title":"Coarse-grained simulations of lysozyme-silica-nanoparticle corona.","authors":"Lanlan Qin, Gaobo Yu, Jian Zhou","doi":"10.1116/6.0002736","DOIUrl":"https://doi.org/10.1116/6.0002736","url":null,"abstract":"Protein coronas, formed by proteins and nanomaterials, have various applications in the biomedical field. Here, large-scale simulations of protein coronas have been carried out by an efficient mesoscopic coarse-grained method with the BMW-MARTINI force field. The effects of protein concentration, size of silica nanoparticles (SNPs), and ionic strength on the formation of lysozyme-SNP coronas are investigated at the microsecond time scale. Simulations results indicate that (i) an increase in the amount of lysozyme is favorable for the conformation stability of adsorbed lysozyme on SNPs. Moreover, the formation of ringlike and dumbbell-like aggregations of lysozyme can further reduce the conformational loss of lysozyme; (ii) for a smaller SNP, the increase of protein concentration exhibits a greater effect on the adsorption orientation of lysozyme. The dumbbell-like lysozyme aggregation is unfavorable for the stability of lysozyme's adsorption orientation; however, the ringlike lysozyme aggregation can enhance the orientation stability; (iii) the increase of ionic strength can reduce the conformation change of lysozyme and accelerate the aggregation of lysozyme during their adsorption process on SNPs. This work provides some insights into the formation of protein coronas and some valuable guidelines for the development of novel biomolecule-NP conjugates.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9513650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silane coupling agent in biomedical materials. 生物医用材料中的硅烷偶联剂。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002712

Ze-Qun Zhang, Ke-Feng Ren, Jian Ji

引用次数: 2

Comparison study of mouse brain tissue by using ToF-SIMS within static limits and hybrid SIMS beyond static limits (dynamic mode). 静态范围内ToF-SIMS与静态范围外混合SIMS(动态模式)对小鼠脑组织的比较研究。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002613

Hyun Kyong Shon, Jin Gyeong Son, Sun Young Lee, Jeong Hee Moon, Ga Seul Lee, Kyoung-Shim Kim, Tae Geol Lee

{"title":"Comparison study of mouse brain tissue by using ToF-SIMS within static limits and hybrid SIMS beyond static limits (dynamic mode).","authors":"Hyun Kyong Shon, Jin Gyeong Son, Sun Young Lee, Jeong Hee Moon, Ga Seul Lee, Kyoung-Shim Kim, Tae Geol Lee","doi":"10.1116/6.0002613","DOIUrl":"https://doi.org/10.1116/6.0002613","url":null,"abstract":"In the study of degenerative brain diseases, changes in lipids, the main component of neurons, are particularly important because they are used as indicators of pathological changes. One method for the sensitive measurement of biomolecules, especially lipids, is time-of-flight secondary ion mass spectrometry (ToF-SIMS) using pulsed argon cluster ions. In this study, biomolecules including various lipids present in normal mouse brain tissue were measured using ToF-SIMS equipped with pulsed argon cluster primary ions. Based on the ToF-SIMS measurement results, hybrid SIMS (OrbiSIMS), which is a ToF-SIMS system with the addition of an orbitrap mass analyzer, was used to directly identify the biomolecules by the region in the real tissue samples. For this, the results of ToF-SIMS, which measured the tissue samples from a single mouse brain within static limits, were compared with those from OrbiSIMS measured beyond the static limits in terms of the differences in molecular profiling. From this analysis, two types of positive and negative ions were selected for identification, with the OrbiSIMS MS/MS results indicating that the positive ions were glycerophosphocholine and the negative ions were glycerophosphoinositol and sulfatide, a sphingolipid. Then, to confirm the identification of the molecular candidates, lipids were extracted from mirror image tissue samples, and LC-MS/MS also using an orbitrap mass analyzer was performed. As a result, the direct identification of molecular candidate groups distributed in particular regions of the tissue samples via OrbiSIMS was found to be consistent with the identification results by LC-MS/MS for extracted samples.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High throughput wide field second harmonic imaging of giant unilamellar vesicles. 巨型单层囊泡的高通量宽场二次谐波成像。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002640

M Eremchev, D Roesel, P-M Dansette, A Michailovas, S Roke

引用次数: 1