Biointerphases最新文献_第8页

Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix. 局部粘附介导的亚细胞曲率传感对工程细胞外基质反应的评估。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-04-05 DOI: 10.1116/6.0002440

Daniel T Bowers, Mary Elizabeth McCulloch, Justin L Brown

{"title":"Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.","authors":"Daniel T Bowers, Mary Elizabeth McCulloch, Justin L Brown","doi":"10.1116/6.0002440","DOIUrl":"10.1116/6.0002440","url":null,"abstract":"Fibril curvature is bioinstructive to attached cells. Similar to natural healthy tissues, an engineered extracellular matrix can be designed to stimulate cells to adopt desired phenotypes. To take full advantage of the curvature control in biomaterial fabrication methodologies, an understanding of the response to fibril subcellular curvature is required. In this work, we examined morphology, signaling, and function of human cells attached to electrospun nanofibers. We controlled curvature across an order of magnitude using nondegradable poly(methyl methacrylate) (PMMA) attached to a stiff substrate with flat PMMA as a control. Focal adhesion length and the distance of maximum intensity from the geographic center of the vinculin positive focal adhesion both peaked at a fiber curvature of 2.5 μm-1 (both ∼2× the flat surface control). Vinculin experienced slightly less tension when attached to nanofiber substrates. Vinculin expression was also more affected by a subcellular curvature than structural proteins α-tubulin or α-actinin. Among the phosphorylation sites we examined (FAK397, 576/577, 925, and Src416), FAK925 exhibited the most dependance on the nanofiber curvature. A RhoA/ROCK dependance of migration velocity across curvatures combined with an observation of cell membrane wrapping around nanofibers suggested a hybrid of migration modes for cells attached to fibers as has been observed in 3D matrices. Careful selection of nanofiber curvature for regenerative engineering scaffolds and substrates used to study cell biology is required to maximize the potential of these techniques for scientific exploration and ultimately improvement of human health.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10661787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Back to the basics of time-of-flight secondary ion mass spectrometry of bio-related samples. I. Instrumentation and data collection. 生物相关样品的飞行时间二次离子质谱分析的基本原理。I. 仪器和数据采集。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-29 DOI: 10.1116/6.0002477

Daniel J Graham, Lara J Gamble

引用次数: 0

3D bioprinting of gastrointestinal cancer models: A comprehensive review on processing, properties, and therapeutic implications. 胃肠道肿瘤模型的3D生物打印:加工、特性和治疗意义的综合综述。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-24 DOI: 10.1116/6.0002372

Kalappa Prashantha, Amita Krishnappa, Malini Muthappa

{"title":"3D bioprinting of gastrointestinal cancer models: A comprehensive review on processing, properties, and therapeutic implications.","authors":"Kalappa Prashantha, Amita Krishnappa, Malini Muthappa","doi":"10.1116/6.0002372","DOIUrl":"https://doi.org/10.1116/6.0002372","url":null,"abstract":"Gastrointestinal tract (GIT) malignancies are an important public health problem considering the increased incidence in recent years and the high morbidity and mortality associated with it. GIT malignancies constitute 26% of the global cancer incidence burden and 35% of all cancer-related deaths. Gastrointestinal cancers are complex and heterogenous diseases caused by the interplay of genetic and environmental factors. The tumor microenvironment (TME) of gastrointestinal tract carcinomas is dynamic and complex; it cannot be recapitulated in the basic two-dimensional cell culture systems. In contrast, three-dimensional (3D) in vitro models can mimic the TME more closely, enabling an improved understanding of the microenvironmental cues involved in the various stages of cancer initiation, progression, and metastasis. However, the heterogeneity of the TME is incompletely reproduced in these 3D culture models, as they fail to regulate the orientation and interaction of various cell types in a complex architecture. To emulate the TME, 3D bioprinting has emerged as a useful technique to engineer cancer tissue models. Bioprinted cancer tissue models can potentially recapitulate cancer pathology and increase drug resistance in an organ-mimicking 3D environment. In this review, we describe the 3D bioprinting methods, bioinks, characterization of 3D bioprinted constructs, and their application in developing gastrointestinal tumor models that integrate their microenvironment with different cell types and substrates, as well as bioprinting modalities and their application in therapy and drug screening. We review prominent studies on the 3D bioprinted esophageal, hepatobiliary, and colorectal cancer models. In addition, this review provides a comprehensive understanding of the cancer microenvironment in printed tumor models, highlights current challenges with respect to their clinical translation, and summarizes future perspectives.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatibility evaluation of polyethersulfone-pyrolytic carbon composite membrane in artificial pancreas. 聚醚砜-热解碳复合膜在人工胰腺中的生物相容性评价。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-21 DOI: 10.1116/6.0002155

Reza Peighami, Mohamadreza Mehrnia, Fatemeh Yazdian, Mojgan Sheikhpour

{"title":"Biocompatibility evaluation of polyethersulfone-pyrolytic carbon composite membrane in artificial pancreas.","authors":"Reza Peighami, Mohamadreza Mehrnia, Fatemeh Yazdian, Mojgan Sheikhpour","doi":"10.1116/6.0002155","DOIUrl":"https://doi.org/10.1116/6.0002155","url":null,"abstract":"Polyethersulfone (PES) membranes are widely used in medical devices, especially intravascular devices such as intravascular bioartificial pancreases. In the current work, the pure PES and PES-pyrolytic carbon (PyC) composite membranes were synthesized and permeability studies were conducted. In addition, the cytocompatibility and hemocompatibility of the pure PES and PES-PyC membranes were investigated. These materials were characterized using peripheral blood mononuclear cell (PBMC) activation, platelet activation, platelet adhesion, ß-cell viability and proliferation, and ß-cell response to hyperglycemia. The results showed that platelet activation decreased from 87.3% to 27.8%. Any alteration in the morphology of sticking platelets was prevented, and the number of attached platelets decreased by modification with PyC. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay corroborated that PBMC activation was encouraged by the PyC-modified PES membrane surface. It can be concluded that PES-modified membranes show higher hemocompatibility than pure PES membranes. ß-cells cultured on all the three membranes displayed a lower rate of proliferation although the cells on the PES-PyC (0.1 wt. %) membrane indicated a slightly higher viability and proliferation than those on the pure PES and PES-PyC (0.05 wt. %) membranes. It shows that the PES-PyC (0.1 wt. %) membrane possesses superior cytocompatibility over the other membranes.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9355620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconjugation of COL1 protein on liquid-like solid surfaces to study tumor invasion dynamics. COL1蛋白在类液体固体表面的生物偶联以研究肿瘤侵袭动力学。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-03-10 DOI: 10.1116/6.0002083

D T Nguyen, D I Pedro, A Pepe, J G Rosa, J I Bowman, L Trachsel, G R Golde, I Suzuki, J M Lavrador, N T Y Nguyen, M A Kis, R A Smolchek, N Diodati, R Liu, S R Phillpot, A R Webber, P Castillo, E J Sayour, B S Sumerlin, W G Sawyer

{"title":"Bioconjugation of COL1 protein on liquid-like solid surfaces to study tumor invasion dynamics.","authors":"D T Nguyen, D I Pedro, A Pepe, J G Rosa, J I Bowman, L Trachsel, G R Golde, I Suzuki, J M Lavrador, N T Y Nguyen, M A Kis, R A Smolchek, N Diodati, R Liu, S R Phillpot, A R Webber, P Castillo, E J Sayour, B S Sumerlin, W G Sawyer","doi":"10.1116/6.0002083","DOIUrl":"10.1116/6.0002083","url":null,"abstract":"Tumor invasion is likely driven by the product of intrinsic and extrinsic stresses, reduced intercellular adhesion, and reciprocal interactions between the cancer cells and the extracellular matrix (ECM). The ECM is a dynamic material system that is continuously evolving with the tumor microenvironment. Although it is widely reported that cancer cells degrade the ECM to create paths for migration using membrane-bound and soluble enzymes, other nonenzymatic mechanisms of invasion are less studied and not clearly understood. To explore tumor invasion that is independent of enzymatic degradation, we have created an open three-dimensional (3D) microchannel network using a novel bioconjugated liquid-like solid (LLS) medium to mimic both the tortuosity and the permeability of a loose capillary-like network. The LLS is made from an ensemble of soft granular microgels, which provides an accessible platform to investigate the 3D invasion of glioblastoma (GBM) tumor spheroids using in situ scanning confocal microscopy. The surface conjugation of the LLS microgels with type 1 collagen (COL1-LLS) enables cell adhesion and migration. In this model, invasive fronts of the GBM microtumor protruded into the proximal interstitial space and may have locally reorganized the surrounding COL1-LLS. Characterization of the invasive paths revealed a super-diffusive behavior of these fronts. Numerical simulations suggest that the interstitial space guided tumor invasion by restricting available paths, and this physical restriction is responsible for the super-diffusive behavior. This study also presents evidence that cancer cells utilize anchorage-dependent migration to explore their surroundings, and geometrical cues guide 3D tumor invasion along the accessible paths independent of proteolytic ability.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10643322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposome functionalized reduced graphene oxide for rapid electrochemical sensing of bacteria. 脂质体功能化还原氧化石墨烯用于细菌的快速电化学传感。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-01 DOI: 10.1116/6.0002376

Shailendra Kumar, Juhi Jaiswal, Snehlata Yadav, Marshal Dhayal

{"title":"Liposome functionalized reduced graphene oxide for rapid electrochemical sensing of bacteria.","authors":"Shailendra Kumar, Juhi Jaiswal, Snehlata Yadav, Marshal Dhayal","doi":"10.1116/6.0002376","DOIUrl":"https://doi.org/10.1116/6.0002376","url":null,"abstract":"Pathogenic bacteria represent a severe threat to global public health, particularly with the growing rate of antibiotic resistance, and, therefore, indicate a critical need for developing efficient sensing platforms. Liposome-based sensors are collocating interest due to their intrinsic fusogenic ability to fuse with the outer membrane of bacteria. However, the lack of a conducting property limits their applicability for developing biosensing platforms. In this study, we report conjugation of liposomes with reduced graphene oxide (rGO) for fabricating a rapid and sensitive biosensor for electrochemical detection of Escherichia coli (E. coli). The large surface area of rGO facilitated binding of liposomes with their surface, and the intrinsic electrical and biocompatible properties assisted electrochemical sensing of bacteria. The electrochemical response of the liposome and the rGO-liposome coated electrode shows nonconducting and conducting characteristics, respectively. A significant change in the peak current of differential pulse voltammetry with the gradual variation of bacterial density in the electrolyte was observed for the glassy carbon electrode rGO-liposome (GCE-L-rGO) surface only. The detection sensitivity of GCE-L-rGO sensors was ∼26 μA/106 cells per ml of electrolyte for varying cell densities from 3 × 103 to 3 × 104 cells/ml. The proposed sensing technique can serve as an alternative to conventional methodologies for rapid and in situ detection of bacterial load in different samples, laying the foundation for new applications in clinical diagnostics.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9383896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of WE43 magnesium alloy by powder metallurgy and the effect of glucose on wear resistance in biocorrosive wear. 粉末冶金法制备WE43镁合金及葡萄糖对生物腐蚀磨损耐磨性的影响

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-01 DOI: 10.1116/6.0002270

Bünyamin Çiçek

引用次数: 0

Polyacrylonitrile nanofibrous membrane composited with zeolite imidazole skeleton-8 and silver nanoclusters for efficient antibacterial and emulsion separation. 以沸石咪唑骨架-8和纳米银团簇复合的聚丙烯腈纳米纤维膜用于高效抗菌和乳液分离。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-03-01 DOI: 10.1116/6.0002615

Huaxiang Chen, Hao Zhou, Mingchao Chen, Yan Quan, Chenglong Wang, Yujie Gao, Jindan Wu

{"title":"Polyacrylonitrile nanofibrous membrane composited with zeolite imidazole skeleton-8 and silver nanoclusters for efficient antibacterial and emulsion separation.","authors":"Huaxiang Chen, Hao Zhou, Mingchao Chen, Yan Quan, Chenglong Wang, Yujie Gao, Jindan Wu","doi":"10.1116/6.0002615","DOIUrl":"https://doi.org/10.1116/6.0002615","url":null,"abstract":"Oily wastewater discharged by industrial development is an important factor causing water pollution. Membrane separation technology has the advantages of low cost, simple operation, and high efficiency in the treatment of oily wastewater. However, membrane materials are easily eroded by microorganisms during long-term storage or use, thereby resulting in reduced separation efficiency. Herein, a zeolite imidazole skeleton-8@silver nanocluster composite polyacrylonitrile (ZIF-8@AgNCs/PAN) nanofibrous membrane was fabricated by electrospinning and in situ growth technology. The surface chemistry, morphology, and wettability of the composite membranes were characterized. The carboxyl groups on the surface of hydrolyzed PAN nanofibers, which can be complexed with zinc ions (Zn2+), are utilized as growth sites for porous metal organic frameworks (ZIF-8). Meanwhile, AgNCs are loaded into ZIF-8 to achieve stable hybridization of ZIF-8@AgNCs and nanofibers. The loading quantity of ZIF-8@AgNCs, which can dominantly affect the surface roughness and the porosity of the membranes, is regulated by the feeding amount of AgNCs. The ZIF-8@AgNCs/PAN membrane achieves effective oil-water separation with high separation efficiency toward petroleum ether-in-water emulsion (98.6%) and permeability (62 456 ± 1343 Lm-2 h-1 bar-1). Furthermore, the ZIF-8@AgNCs/PAN membrane possesses high antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus), which is beneficial for the long-term storage and use of the membrane.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atomistic simulations for investigation of substrate effects on lipid in-source fragmentation in secondary ion mass spectrometry. 二级离子质谱法研究底物对脂质源内碎片影响的原子模拟。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-02-09 DOI: 10.1116/6.0002298

Michael J Taylor, Hoshin Kim, William Kew, Amity Andersen, Arunima Bhattacharjee, Mark H Engelhard, Christopher R Anderton

{"title":"Atomistic simulations for investigation of substrate effects on lipid in-source fragmentation in secondary ion mass spectrometry.","authors":"Michael J Taylor, Hoshin Kim, William Kew, Amity Andersen, Arunima Bhattacharjee, Mark H Engelhard, Christopher R Anderton","doi":"10.1116/6.0002298","DOIUrl":"https://doi.org/10.1116/6.0002298","url":null,"abstract":"In beam-based ionization methods, the substrate plays an important role on the desorption mechanism of molecules from surfaces. Both the specific orientation that a molecule adopts at a surface and the strength of the molecule-surface interaction can greatly influence desorption processes, which in turn will affect the ion yield and the degree of in-source fragmentation of a molecule. In the beam-based method of secondary ion mass spectrometry (SIMS), in-source fragmentation can be significant and molecule specific due to the hard ionization method of using a primary ion beam for molecule desorption. To investigate the role of the substrate on orientation and in-source fragmentation, we have used atomistic simulations-molecular dynamics in combination with density functional theory calculations-to explore the desorption of a sphingolipid (palmitoylsphingomyelin) from a model surface (gold). We then compare SIMS data from this model system to our modeling findings. Using this approach, we found that the combined adsorption and binding energy of certain bonds associated with the headgroup fragments (C3H8N+, C5H12N+, C5H14NO+, and C5H15PNO4 +) was a good predictor for fragment intensities (as indicated by relative ion yields). This is the first example where atomistic simulations have been applied in beam-based ionization of lipids, and it presents a new approach to study biointerfacial lipid ordering effects on SIMS imaging.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9287281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing the human epidermis by combining ToF-SIMS and multivariate analysis. 结合ToF-SIMS和多变量分析对人体表皮进行探测。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-02-08 DOI: 10.1116/6.0002289

Xavier Delvaux, Céline Noël, Yves Poumay, Laurent Houssiau

{"title":"Probing the human epidermis by combining ToF-SIMS and multivariate analysis.","authors":"Xavier Delvaux, Céline Noël, Yves Poumay, Laurent Houssiau","doi":"10.1116/6.0002289","DOIUrl":"https://doi.org/10.1116/6.0002289","url":null,"abstract":"The mammalian organism is continuously exposed to various biological and chemical threats from its surroundings. In order to provide protection against these threats, mammals have developed a specialized defense system at the interface with their environment. This system, known as the epidermis, is mainly composed of stratified keratinocytes organized in a complex self-renewing structure providing a mechanical and chemical barrier at the skin surface. However, numerous skin-related pathologies can interfere with the proper formation and function of the epidermal barrier. The pathogenesis of these alterations is often very complex. Understanding the changes induced in epidermal tissues by these pathologies at a molecular level is key for their treatment and prevention. In this context, this work aims at developing a thorough and reproducible characterization methodology of the human epidermis by applying ToF-SIMS to the study of an in vitro epidermal model known as reconstructed human epidermis (RHE). Indeed, although the potential of ToF-SIMS for the characterization of the mammalian skin has already been demonstrated, very few studies focus their efforts on the human epidermis itself. Here, we performed static ToF-SIMS characterizations of RHE cryosections, combining both high mass and high lateral resolution acquisitions. In addition, principal components analysis was used as a multivariate analysis tool. This contributed to the decorrelation of the complex datasets obtained from these biological systems and allowed capturing of their most statistically representative spectral features. Remarkably, this tool proved to be successful in extracting meaningful biological information from the datasets by yielding principal components distinguishing the cornified layers from the metabolically active epidermal cells. Finally, on the basis of multiple ToF-SIMS acquisitions, we showed that this methodology allows for the convenient production of experimental replicates, a key feature often difficult to achieve in ex vivo approaches.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10725969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0