真空暴露对质谱分析样品稳定性的影响。

IF 1.9 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2025-07-01 DOI:10.1116/6.0004632
Matija Lagator, Siyu Liu, C Logan Mackay, Felicia Green
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引用次数: 0

摘要

质谱(MS)通常需要真空条件,这虽然有利于分析,但可能不可预测地改变敏感样品。本研究探讨了长时间真空暴露对二次离子质谱(SIMS)对乙酰氨基酚薄膜质谱检测一致性和可靠性的影响。在室温真空条件下,在实验持续时间(42 h)内,质谱信号强度下降了约81.5%。光学显微镜显示,这种减少与升华引起的对乙酰氨基酚的样品损失一致。因此,对乙酰氨基酚在底物上的覆盖变得不均匀,导致SIMS信号中的相对标准偏差(RSD)随时间增加。相比之下,在低温条件下,没有观察到信号退化或RSD增加。此外,与大气压质谱法的比较表明,在没有真空的情况下,信号强度随着时间的推移保持更稳定。这些发现突出了真空暴露对挥发性标准的潜在缺点,并强调了在分析之前测试真空效应的重要性。如果需要真空,应考虑低温条件以减轻样品降解。虽然这些影响是在质谱技术中观察到的,但它们也适用于任何类型的基于真空的方法,其中样品可能容易升华。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of vacuum exposure on sample stability for mass spectrometry analysis.

Mass spectrometry (MS) often requires vacuum conditions, which, while beneficial for analysis, can unpredictably alter sensitive samples. This study investigates the impact of prolonged vacuum exposure on the consistency and reliability of MS detection of thin films of acetaminophen using secondary ion mass spectrometry (SIMS). Under vacuum at room temperature, the mass spectrometry signal intensity decreased by approximately 81.5% over the duration of the experiment (42 h). Optical microscopy revealed that this decrease coincided with sublimation-induced sample loss of the acetaminophen. As a result, acetaminophen coverage across the substrate became heterogeneous, leading to increased relative standard deviation (RSD) in the SIMS signal over time. In contrast, under cryogenic conditions, neither signal degradation nor an increase in RSD was observed. Additionally, a comparison with atmospheric pressure mass spectrometry revealed that, in the absence of vacuum, signal intensity remained more stable over time. These findings highlight the potential drawbacks of vacuum exposure for volatile standards and emphasize the importance of testing vacuum effects prior to analysis. If vacuum is necessary, cryogenic conditions should be considered to mitigate sample degradation. While these effects were observed for a mass spectrometry technique, they are also applicable to any type of vacuum-based methodology where the samples might be prone to sublimation.

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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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