Biointerphases最新文献_第2页

Surface potential modulates fibronectin adsorption and molecular interaction on graphene-based materials. 表面电位调节石墨烯基材料上纤维连接蛋白的吸附和分子相互作用。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004504

Rohit, Rachayita Bharadwaj, Chandrashish Roy, Sourabh Ghosh, Sachin Kumar

{"title":"Surface potential modulates fibronectin adsorption and molecular interaction on graphene-based materials.","authors":"Rohit, Rachayita Bharadwaj, Chandrashish Roy, Sourabh Ghosh, Sachin Kumar","doi":"10.1116/6.0004504","DOIUrl":"https://doi.org/10.1116/6.0004504","url":null,"abstract":"Protein interactions on graphene-based materials (GBMs) are predominantly governed by interphase surface properties such as surface chemistry and roughness; however, the critical role of surface potential (SP) in modulating these interactions remains largely unexplored. In this work, we investigated a model study highlighting how two distinct GBMs [graphene oxide (GO) and reduced graphene oxide (RGO)] with different SP regulate protein interactions, spanning from macroscopic adsorption to molecular-level conformational changes. Through thermal reduction, hydrophilic GO was transformed into hydrophobic RGO, generating distinct SP of +120 mV for GO and +60 mV for RGO. This modulation in SP created a platform for differential protein interactions. The influence of SP on protein interactions was evident when fibronectin (FN) was introduced onto GO and RGO surfaces. Quartz crystal microbalance with dissipation and fluorescence microscopy revealed that the distinct SP of GO and RGO surfaces significantly affected FN adsorption. On the RGO substrate, which exhibited a lower SP, FN adsorption was ∼3 times greater than on the GO substrate. In contrast, FN on the GO adopted elongated fibrillar structures, driven by strong polar, hydrophilic, and electrostatic interactions at the molecular scale, regulating its conformation upon adsorption. Molecular docking simulations further supported these findings, indicating a stronger and more stable interaction between FN and RGO (binding energy C-score: -3.87, RMSD: 0.01 Å) than between FN and GO (C-score: -2.24, RMSD: 0.42 Å). Overall, this study underscores the pivotal role of SP of GBMs in modulating protein adsorption, binding stability, and conformational organization, providing key insights into the rational design of GBM biomaterials with tailored biointerface properties.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: "Performance of biological food processing interfaces: Perspectives on the science of mollusc radula" [Biointerphases 19, 030801 (2024)]. 勘误：“生物食品加工界面的性能：对软体动物radula科学的看法”[生物界面，19,030801(2024)]。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004726

Wencke Krings, Stanislav N Gorb

引用次数: 0

In vitro assessment of polyethylene glycol-coated iron oxide nanoparticles integrating luteinizing hormone releasing-hormone targeted magnetic hyperthermia and doxorubicin for lung and breast cancer cells. 聚乙二醇包被氧化铁纳米颗粒整合促黄体生成素释放激素靶向磁热疗和阿霉素治疗肺癌和乳腺癌细胞的体外评估。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004228

Neha Srivastava, Bhupendra Chudasama, Manoj Baranwal

{"title":"In vitro assessment of polyethylene glycol-coated iron oxide nanoparticles integrating luteinizing hormone releasing-hormone targeted magnetic hyperthermia and doxorubicin for lung and breast cancer cells.","authors":"Neha Srivastava, Bhupendra Chudasama, Manoj Baranwal","doi":"10.1116/6.0004228","DOIUrl":"https://doi.org/10.1116/6.0004228","url":null,"abstract":"Magnetic nanoparticle-based targeted hyperthermia, combined with chemotherapy, is a promising approach for cancer treatment. In this study, a targeted magnetic drug delivery system was developed, comprising doxorubicin (DOX), a [D-Trp6] luteinizing hormone-releasing hormone (LHRH) (Triptorelin) ligand, and a polyethylene glycol (PEG)-coated magnetite core, aiming to enhance cancer therapy efficacy. Fourier-transform infrared spectroscopy confirmed the conjugation of LHRH onto the PEG-coated Fe3O4 nanoparticles. Ultraviolet-visible spectroscopy was employed to assess drug loading, revealing a loading efficiency of 66%. The DOX-loaded, LHRH-tagged PEG-coated Fe3O4 nanoparticles were evaluated for their cytotoxic effects on A549 and MCF-7 cancer cell lines under three treatment modalities: thermotherapy, chemotherapy, and combined thermo-chemotherapy, both with and without the application of a magnetic field. Cell viability was assessed using the 2,5-diphenyltetrazolium bromide (MTT) assay. In A549 cells, the combined thermo-chemotherapy treatment at a DOX concentration of 10 μg/ml resulted in an 88% reduction in cell viability, outperforming chemotherapy alone (62%) and thermotherapy alone (47%). Similarly, in MCF-7 cells, the combined treatment at 8 μg/ml DOX led to a 91% reduction in viability, surpassing the effects of chemotherapy (57%) and thermotherapy (45%) individually. Additionally, the targeted DOX-loaded nanoparticles significantly elevated interferon-gamma production, indicating an enhanced immune response and increased cancer cell apoptosis.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing epithelial tissue sealing of titanium alloy implants through CaCl2-based hydrothermal treatment. 基于cacl2的热液处理增强钛合金植入物上皮组织的密封性。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-03-01 DOI: 10.1116/6.0004359

Yasushige Sakamoto, Akihiro Furuhashi, Albert Mufundirwa, Takeharu Sugiyama, Ikiru Atsuta, Yasunori Ayukawa

{"title":"Enhancing epithelial tissue sealing of titanium alloy implants through CaCl2-based hydrothermal treatment.","authors":"Yasushige Sakamoto, Akihiro Furuhashi, Albert Mufundirwa, Takeharu Sugiyama, Ikiru Atsuta, Yasunori Ayukawa","doi":"10.1116/6.0004359","DOIUrl":"https://doi.org/10.1116/6.0004359","url":null,"abstract":"The long-term stability of dental implants is significantly influenced by their resistance to foreign factors in the peri-implant epithelium (PIE). Despite this, enhancing the sealing properties at the implant-PIE interface continues to be an unmet clinical need. Ti-6Al-4V (Ti64) alloy has higher tensile strength and hardness than pure titanium. This study was to verify whether hydrothermal treatment of Ti64 alloy implants with distilled water (HT-DW) or calcium chloride (CaCl2) solution (HT-Ca) could improve the sealing of the PIE around Ti64 implants. The existence of calcium (Ca) on the surface of HT-Ca implants was confirmed using x-ray photoelectron spectroscopy and synchrotron-based x-ray absorption fine structure techniques. These data showed that the surface was oxidized, and Ca existed in the form of anhydrous CaCl2 and calcium titanate. Laminin-332 (Ln), which is an essential component of epithelial adhesion structures, was observed between all types of implants and the PIE, 4 weeks after implantation in rat maxillae. Ln distribution over the entire epithelial interface was similar for the HT-Ca implant and a natural tooth. Moreover, the HT-Ca implant inhibited foreign body penetration, which indicated stronger gingival sealing at the implant-PIE interface, compared with the untreated and HT-DW implants. We also investigated the attachment of mouse-derived gingival epithelial cells (GE1). GE1 adherence was stronger and Ln expression levels were higher for HT-Ca plates compared with the untreated and HT-DW plates. Our results demonstrate that hydrothermal treatment of Ti64 implants with CaCl2 solution facilitates the growth of an effective soft tissue seal around the implant.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 2","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of targeting nanogels for controlled delivery of 5-aminolevulinic acid triggered by matrix metalloproteinases as photodynamic therapy. 光动力疗法中基质金属蛋白酶触发的5-氨基乙酰丙酸靶向递送纳米凝胶的制备

IF 1.6 4区医学

Biointerphases Pub Date : 2025-03-01 DOI: 10.1116/6.0004203

Xiao Liu, Yuan Zhang, Peng Zhang, Kang Ge, Ruzhi Zhang, Yixin Sun, Yang Sheng, Mark Bradley, Rong Zhang

{"title":"Preparation of targeting nanogels for controlled delivery of 5-aminolevulinic acid triggered by matrix metalloproteinases as photodynamic therapy.","authors":"Xiao Liu, Yuan Zhang, Peng Zhang, Kang Ge, Ruzhi Zhang, Yixin Sun, Yang Sheng, Mark Bradley, Rong Zhang","doi":"10.1116/6.0004203","DOIUrl":"10.1116/6.0004203","url":null,"abstract":"5-Aminolevulinic acid (5-ALA) is a prodrug of the photodynamic therapy (PDT) for the treatment of certain skin diseases and neuronal cancers in the clinic. However, it is difficult for 5-ALA to target specific cells and, therefore, to accumulate within deeper lesions, leading to poor conversion of protoporphyrin IX, the active photodynamic agent. To solve this problem, targeted nanogels were developed for controlled 5-ALA delivery. Here, nanogels with folic acid as a targeting ligand were prepared by inverse microemulsion polymerization using the peptide cross-linker acryl-PLGLAGK(Alloc)-NH2, a generic substrate for matrix metalloproteinases, enzymes associated with many tumors. The stability, entrapment efficiency, drug loading, and drug release ability of the nanogels were studied with skin cancer cells (A2058) and showed that the targeting nanogels enhanced the concentration of 5-ALA in tumor cells and improved the efficiency of PDT in vitro. In vivo experiments showed that the targeting nanogels loaded with 5-ALA dramatically inhibited the development of skin cancer.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 2","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical study on the application effect of improved polyetheretherketone cranial plate in cranioplasty. 改良聚醚酮颅板在颅骨成形术中的应用效果研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004208

Jiajun Qin, Fei Xue, Jin Fu, Jiping Sun

引用次数: 0

Erratum: "Linking drivers of plant per- and polyfluoroalkyl substances (PFAS) uptake to agricultural land management decisions" [Biointerphases 18, 040801 (2023)]. 勘误：“将植物全氟和多氟烷基物质（PFAS）吸收的驱动因素与农业土地管理决策联系起来”[生物界面期18,040801(2023)]。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004421

Alex E Scearce, Caleb P Goossen, Rachel E Schattman, Ellen B Mallory, Jean D MacRae

引用次数: 0

Applications and latest research progress of liposomes in the treatment of ocular diseases. 脂质体在眼部疾病治疗中的应用及最新研究进展。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004159

Huan Tang, Xinnan Li, Lin Jin, Jicheng Dong, Li Yang, Chunmei Li, Lijun Zhang, Fang Cheng

{"title":"Applications and latest research progress of liposomes in the treatment of ocular diseases.","authors":"Huan Tang, Xinnan Li, Lin Jin, Jicheng Dong, Li Yang, Chunmei Li, Lijun Zhang, Fang Cheng","doi":"10.1116/6.0004159","DOIUrl":"https://doi.org/10.1116/6.0004159","url":null,"abstract":"The special structure of eyes and the existence of various physiological barriers make ocular drug delivery one of the most difficult problems in the pharmaceutical field. Considering the problems of patient compliance, local administration remains the preferred method of drug administration in the anterior part of eyes. However, local administration suffers from poor bioavailability, need for frequent administration, and systemic toxicity. Administration in the posterior part of the eye is more difficult, and intravitreal injection is often used. But intravitreal injection faces the problems of poor patient compliance and likely side effects after multiple injections. The development of nanocarrier technology provides an effective way to solve these problems. Among them, liposomes, as the most widely used carrier in clinical application, have the characteristics of amphiphilic nanostructure, easy surface modification, extended release time, good biocompatibility, etc. The liposomes are expected to overcome obstacles and effectively deliver drugs to the target site to improve ocular drug bioavailability. This review summarized the various controllable properties of liposomes for ocular delivery as well as the application and research progress of liposomes in various ocular diseases. In addition, we summarized the physiological barriers and routes of administration contained in eyes, as well as the prospects of liposomes in the treatment of ocular diseases.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photon statistics and unicellular algae cultivation characteristics under continuous illumination. 连续光照下的光子统计与单细胞藻类培养特性研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004347

Yair Zarmi

引用次数: 0

Electrochemical study of oxidized hyaluronic acid grafted with aniline tetramer Bletilla striata polysaccharide hydrogel wound dressings. 氧化透明质酸接枝苯胺四聚体白芨多糖水凝胶伤口敷料的电化学研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004108

Li Luo, Xiaofei Wang, Li Ma, Kaibin Wang, Jiandong Ma, Xiaoliang Zhao, Yonggang Wang, Jing Zhang, Cunjin Wang, Weijie Zhang

{"title":"Electrochemical study of oxidized hyaluronic acid grafted with aniline tetramer Bletilla striata polysaccharide hydrogel wound dressings.","authors":"Li Luo, Xiaofei Wang, Li Ma, Kaibin Wang, Jiandong Ma, Xiaoliang Zhao, Yonggang Wang, Jing Zhang, Cunjin Wang, Weijie Zhang","doi":"10.1116/6.0004108","DOIUrl":"10.1116/6.0004108","url":null,"abstract":"Bletilla striata polysaccharide, also known as Bletilla gum, is a water-soluble polymeric viscous polysaccharide with antimicrobial and coagulation-promoting activities. This study used Bletilla striata polysaccharide as the base material and crosslinked it with an electroactive carrier material to prepare a hydrogel with both conductivity and bioactivity. Specifically, hydroxypropyl chitosan with good bioactivity was used as a cross-linking agent. Through Schiff base reaction, oxidized hyaluronic acid grafted with aniline tetramer copolymer and oxidized Bletilla striata polysaccharide were crosslinked to prepare conductive hydrogels, and their properties were characterized. Comparative results indicate that the oxidized Bletilla striata polysaccharide-hyaluronic acid conductive hydrogel (HP/OB-OT4) prepared with 20% grafted conductive polymer aniline tetramer content exhibits good conductivity, with an electrical conductivity reaching 0.32 ± 0.013 mS/cm, meeting the requirements for microcurrent stimulation. It also shows a degradation rate of 58.46 ± 1.96% and possesses good antibacterial properties and biocompatibility, demonstrating potential for application in biomedical material fields such as wound dressings.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0