Biointerphases最新文献_第2页

Effect of chain length of imidazolium ionic liquids on the l-phenylalanine induced phospholipid vesicle membranes: Fluorescence lifetime imaging microscopy study. 咪唑类离子液体链长对l-苯丙氨酸诱导磷脂囊泡膜的影响：荧光寿命成像显微镜研究。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004554

Nanigopal Bera, Sandip Mondal, Arijit Maity, Pratyush Kiran Nandi, Nilmoni Sarkar

引用次数: 0

Bacterial motility in aqueous micro-environment with natural soil particles. 天然土壤颗粒在水微环境中的细菌运动。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004380

Diksha Shrestha, Kishan Mahmud, Sam Mortenson, Mary Savin, Wen Zhang, Yong Wang

{"title":"Bacterial motility in aqueous micro-environment with natural soil particles.","authors":"Diksha Shrestha, Kishan Mahmud, Sam Mortenson, Mary Savin, Wen Zhang, Yong Wang","doi":"10.1116/6.0004380","DOIUrl":"https://doi.org/10.1116/6.0004380","url":null,"abstract":"Bacterial motility is essential for navigating heterogeneous environments like soil, where it plays a key role in nutrient cycling, bioremediation, and overall soil health. Despite its importance, the interplay between bacterial motility and soil microstructures-such as the effects of physical confinement and interfacial interactions-remains underexplored. In this study, we investigated the motility of Escherichia coli bacteria in aqueous micro-environments with three different natural soil samples and examined how the particle size, void fraction, and proximity to soil particles affect bacterial motility and movement patterns by quantitatively analyzing bacterial trajectories, velocities, and directional changes. We observed that bacterial velocity decreased significantly in soil micro-environments, showing a strong positive correlation with the soil particle size and a negative correlation with the void fraction of the soil samples. Additionally, bacteria in soil micro-environments showed rapid and dramatic directional changes, and the rate of directional changes of bacteria was negatively correlated with the particle size. These results were further validated with synthetic micro-environments with glass microspheres. As the density of microspheres increased, the translational velocity of bacteria decreased while the directional changes increased. This study enhances our understanding of how the soil type, porosity, and particle proximity impact bacterial movement and is expected to contribute to a better understanding of bacterial activities on soil health and management.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New way to isolate cultured cell exosomes. 分离培养细胞外泌体的新方法。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004479

İshak Afşin Kariper, Nilgün Okşak, Dilek Bahar

引用次数: 0

Insight into the role of the chemical nature and length of the loop nucleobases in the folding of G-quadruplex by the antimalarial drugs at the DNA interface. 抗疟疾药物在DNA界面上折叠g -四重体时环核碱基的化学性质和长度的作用。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004389

Asim Bisoi, Prashant Chandra Singh

{"title":"Insight into the role of the chemical nature and length of the loop nucleobases in the folding of G-quadruplex by the antimalarial drugs at the DNA interface.","authors":"Asim Bisoi, Prashant Chandra Singh","doi":"10.1116/6.0004389","DOIUrl":"https://doi.org/10.1116/6.0004389","url":null,"abstract":"G-quadruplexes (G4) have been proposed as an alternative target for cancer therapy, as the folding of DNA sequences into stabilized G4 in the cancer microenvironment affects key biological functions. The antimalarial drugs, hydroxychloroquine (HCQ) and chloroquine (CQ), are in the clinical trial stage for cancer therapy and have been found to fold DNA sequences into the stabilized G4 even in the absence of KCl salt. In this study, the role of loop nucleobases in terms of chemical nature, number, and location in the HCQ-/CQ-induced folding of DNA sequences into G4 in the absence of KCl has been investigated systematically. The data indicate that both drugs selectively induce the folding of DNA sequences into G-quadruplexes (G4) that contain thymine loop nucleobases. The folding tendency of DNA sequences into stabilized G4 decreases with the increase in the thymine loop nucleobases. Moreover, DNA sequences with fewer thymine loop nucleobases tend to fold into stable G4 when the thymine residues are present at the terminal positions, whereas sequences with more thymine loop nucleobases show higher G4 folding propensity when these bases are located at the central loop. These findings are important in understanding the anticancer effect of antimalarial drugs.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate viscoelasticity regulates fibroblast adhesion and migration. 底物粘弹性调节成纤维细胞粘附和迁移。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004585

Neha Paddillaya, Akshar Rao, Anshul Shrivastava, Imnatoshi Jamir, Kundan Sengupta, Namrata Gundiah

{"title":"Substrate viscoelasticity regulates fibroblast adhesion and migration.","authors":"Neha Paddillaya, Akshar Rao, Anshul Shrivastava, Imnatoshi Jamir, Kundan Sengupta, Namrata Gundiah","doi":"10.1116/6.0004585","DOIUrl":"10.1116/6.0004585","url":null,"abstract":"Mechanical properties of the extracellular matrix (ECM) modulate cell-substrate interactions and influence cellular behaviors such as contractility, migration, and proliferation. Although the effects of substrate stiffness on mechanobiology have been well studied, the role of ECM viscoelasticity in fibrotic progression remains less understood. To examine how viscoelasticity affects the biophysical properties and regulates signaling of human mammary fibroblasts, we engineered elastic (E) and viscoelastic (VE) polyacrylamide hydrogels with comparable storage moduli (∼14.52 ± 1.03 kPa) but distinctly different loss moduli; mean loss moduli for VE gels was 36.9% higher at 0.05 Hz than E gels. Fibroblasts cultured on E hydrogels spread extensively (2428.93 ± 864.71 μm2), developed prominent stress fibers with higher zyxin intensity, and generated higher traction stresses (2931.57 ± 1732.61 Pa). In contrast, fibroblasts on VE substrates had 54.2% smaller focal adhesion areas, exhibited 51.8% lower critical adhesion strengths, and generated 21% lower traction stresses (p < 0.001). These substrates also promoted migration and showed enhanced proliferation with reduced Yes-associated protein (YAP) activity, suggesting a mechanotransduction shift that may involve alternative signaling pathways. In contrast, E substrates showed YAP nuclear translocation, consistent with greater cytoskeletal tension and contractility. These findings highlight the importance of energy dissipation mechanisms in regulating fibroblast function on substrates mimicking the fibrotic milieu. Our results demonstrate that tuning the ECM viscoelasticity is a useful strategy to regulate cell behaviors in tissue-engineered scaffolds and develop better disease modeling for regenerative medicine.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tryptophan-stabilized gold-manganese oxide nanocomposites for enhanced superoxide dismutase activity. 色氨酸稳定金锰氧化物纳米复合材料增强超氧化物歧化酶活性。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004563

Kanika Bharti, Kalyan K Sadhu

{"title":"Tryptophan-stabilized gold-manganese oxide nanocomposites for enhanced superoxide dismutase activity.","authors":"Kanika Bharti, Kalyan K Sadhu","doi":"10.1116/6.0004563","DOIUrl":"https://doi.org/10.1116/6.0004563","url":null,"abstract":"Syntheses of gold-manganese oxide nanocomposites were attempted by a redox-mediated growth method under varying mild reaction conditions with amino acid as a stabilization agent; finally, the nanocomposites were applied for superoxide dismutase (SOD)-mimic activity. Growth reaction was performed by the reduction of Au(III) with Mn(0) powder on the surface of citrate-stabilized gold nanoparticles as seeds. Variable reaction conditions were attempted to monitor the effect of the pH and, finally, optimized based on the critical properties of the nanocomposites including their long-term stability. In a neutral medium, tryptophan-stabilized Au-Mn3O4 nanocomposites were obtained. Stable Au-Mn2O3 nanocomposites were formed at basic pH in the presence of hydrophobic amino acids. The present work elucidates the role of amino acids, especially tryptophan, in stabilizing gold-manganese oxide nanocomposites. The effect of crystalline vs. the amorphous nature of Mn3O4 sheets in the tryptophan-stabilized nanocomposites was evaluated in SOD-mimetic applications. The IC50 values for the newly synthesized Au-Mn3O4 nanocomposites with crystalline or amorphous Mn3O4 sheets at room temperature were found to be 125 times and 25 times better with respect to the reported Mn3O4 nanoparticles synthesized after calcination at 600 °C. These results provide useful insights into the synthesis of gold-manganese oxide nanocomposites with tunable properties and their potential applications in the growing field of nanozymes.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial mapping of the conformational and mechanical properties of bacterial surface biopolymers. 细菌表面生物聚合物构象和力学性质的空间映射。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004587

Asma Eskhan, Somayeh Ramezanian, Samuel Uzoechi, Nehal I Abu-Lail

{"title":"Spatial mapping of the conformational and mechanical properties of bacterial surface biopolymers.","authors":"Asma Eskhan, Somayeh Ramezanian, Samuel Uzoechi, Nehal I Abu-Lail","doi":"10.1116/6.0004587","DOIUrl":"10.1116/6.0004587","url":null,"abstract":"Forces acting between an atomic force microscopy silicon nitride cantilever and the bacterial surface biopolymers of Escherichia coli or Pseudomonas putida were spatially probed in water. The interactions were fitted to a model of steric repulsion to estimate the bacterial surface biopolymer brush length and grafting density. The forces were further fitted to a Hertz model of contact mechanics modified by Sneddon et al. to quantify Young's modulus of elasticity for the cells. Contour plots of the quantified properties described above (i.e., the bacterial surface biopolymer brush length and grafting density, and Young's modulus of elasticity for the cells) based on the location coordinates on the bacterial surfaces were generated. Our contour plots indicated the bacterial cells organize their biopolymers uniquely to help them survive in the environment. Specifically, our results showed that the perimeter of a bacterial cell is characterized by a more flexible as well as longer biopolymer brush compared to those estimated at the center top of the cell. These results suggest that bacteria are likely to use their longer brushes on the edges to facilitate their adhesion by bridging surfaces. Also, they maintain their structural reinforcement by developing higher densities of grafted biopolymers and hence higher elasticities at their centers. Moreover, a stronger linear relationship was observed between the brush thicknesses and the grafting densities for the collapsed brush at the center of the cells when compared to the perimeter of the cells.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in materials research related to orbital reconstruction: A review. 眼眶重建材料研究进展综述。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004390

Yu Sheng, Fangkun Zhao, Tongtong Niu, Jun Xu

引用次数: 0

Integrative analysis of single-cell and bulk transcriptomics reveals necroptosis signatures and immune landscape in hepatoblastoma. 单细胞和整体转录组学的综合分析揭示了肝母细胞瘤的坏死特征和免疫景观。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004611

Weizhou Qiao, Yue Liu, Qinglong Kong, Xiaofeng Tao

{"title":"Integrative analysis of single-cell and bulk transcriptomics reveals necroptosis signatures and immune landscape in hepatoblastoma.","authors":"Weizhou Qiao, Yue Liu, Qinglong Kong, Xiaofeng Tao","doi":"10.1116/6.0004611","DOIUrl":"10.1116/6.0004611","url":null,"abstract":"Hepatoblastoma (HB) is a rare and aggressive pediatric liver tumor with complex etiology. Although necroptosis has been implicated in various cancers, its role in HB remains unclear. This study aimed to investigate the involvement of necroptosis-related genes and immune landscape in HB using integrative bioinformatics and machine learning approaches. Gene expression data from two independent HB datasets were integrated and analyzed. Differentially expressed genes (DEGs) and necroptosis-related DEGs (NR-DEGs) were identified, followed by functional enrichment analysis. Machine learning algorithms were employed to identify hub NR-DEGs. The immune landscape and hub NR-DEGs were investigated using single-sample gene set enrichment analysis (ssGSEA). A total of 1330 upregulated and 1061 downregulated common DEGs were identified. Five upregulated and fourteen downregulated NR-DEGs were identified, which were mainly enriched in immune-related pathways. Four hub NR-DEGs (SLC25A6, HSP90AB1, USP21, and CAMK2B) were identified as potential diagnostic biomarkers for HB. Immune infiltration analysis revealed elevated proportions of CD56bright natural killer cells and gamma delta T cells in HB patients, which significantly correlated with hub NR-DEG expression. ssGSEA indicated that hub NR-DEGs regulate various cellular processes, including cell cycle progression, RNA metabolism, protein synthesis, and viral infection response in HB. This study reveals the involvement of necroptosis-related genes and altered immune infiltration in HB pathogenesis, providing novel insights and potential therapeutic targets.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-of-flight secondary ion mass spectrometry investigation of the influence of ambient air exposure on the migration of brain lipids in a matrix commonly used in matrix assisted laser desorption ionization mass spectrometry imaging. 飞行时间二次离子质谱法研究环境空气暴露对基质中脑脂质迁移的影响，该基质通常用于基质辅助激光解吸电离质谱成像。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-05-01 DOI: 10.1116/6.0004469

Thorsten Adolphs, Michael Bäumer, Richard E Peterson, Heinrich F Arlinghaus, Bonnie J Tyler

{"title":"Time-of-flight secondary ion mass spectrometry investigation of the influence of ambient air exposure on the migration of brain lipids in a matrix commonly used in matrix assisted laser desorption ionization mass spectrometry imaging.","authors":"Thorsten Adolphs, Michael Bäumer, Richard E Peterson, Heinrich F Arlinghaus, Bonnie J Tyler","doi":"10.1116/6.0004469","DOIUrl":"10.1116/6.0004469","url":null,"abstract":"Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging are established techniques for the imaging of biological samples. MALDI-MSI uses organic matrices to enhance desorption and ionization of biomolecules. Before analysis, the sample of interest must be coated with a matrix and the analytes must migrate into the matrix in order for molecular ion signals to be observed. The mechanisms involved in this migration and the sample handling procedures that influence this migration are not well understood. This leads to problems with reproducibility and accuracy of the images. In this study, ToF-SIMS was used to study the effects of exposure to mild ambient environmental conditions on migration of analytes in an α-Cyano-4-hydroxycinnamic acid (CHCA) matrix layer. A mouse brain section was coated with CHCA using an in-house built vapor deposition system and transferred to the ToF-SIMS instrument without breaking vacuum. The brain section was analyzed with ToF-SIMS immediately after vacuum transfer to the instrument, after 24 h storage in vacuum, and following a series of exposures to environmental conditions commonly observed in ambient laboratory air. The redistribution of lipids was observed to be dependent on the laboratory air humidity with minimal migration of most lipids below 50% relative humidity. Different migration behaviors were observed for different lipids as well as for different tissue types. The data show a complex multicomponent process of interdiffusion of the matrix and the brain lipids.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 3","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0