Biointerphases最新文献_第2页

Surface functionalization of polycaprolactone-based biomaterials: Good practice and pitfalls. 聚己内酯基生物材料的表面功能化：良好的实践和缺陷。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004773

Alexandra L Mutch, María Natividad Gómez-Cerezo, Lisbeth Grøndahl

{"title":"Surface functionalization of polycaprolactone-based biomaterials: Good practice and pitfalls.","authors":"Alexandra L Mutch, María Natividad Gómez-Cerezo, Lisbeth Grøndahl","doi":"10.1116/6.0004773","DOIUrl":"https://doi.org/10.1116/6.0004773","url":null,"abstract":"Poly(ɛ-caprolactone) (PCL) remains widely studied in biomaterials science and biomedical engineering due to its versatility and applicability in regenerating a range of tissues including bone, cartilage, neural, and cardiovascular. Due to the hydrophobicity of PCL, most PCL based systems for tissue regeneration require a surface modification process to enhance its in vitro and in vivo compatibility. This Perspective aims to provide an overview of recent strategies used to modify 2D films and 3D scaffolds and the associated methods used to characterize these surfaces. The scope is restricted to physical and chemical postmodification methods, excluding blends and composites, to better isolate the effects of surface chemistry. By analyzing the latest studies (published in 2022-2024), we classified the most commonly employed surface modification techniques, and we identified that the surface evaluation of tailored PCL remains a critical challenge in terms of both chemical and morphological characterization as well as the stability of the introduced surface layer/coating. This status of recent literature highlights current excellent practices and characterization methodologies and suggests approaches for refining surface engineering methods of PCL-based biomaterials in the future.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging biomedical applications of herbal extracts-based biomaterials. 基于草药提取物的生物材料的新兴生物医学应用。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004748

Jianling Mo, Haolu Shi, Kefeng Ren, Zhaoyang Chen, Xia Sheng

{"title":"Emerging biomedical applications of herbal extracts-based biomaterials.","authors":"Jianling Mo, Haolu Shi, Kefeng Ren, Zhaoyang Chen, Xia Sheng","doi":"10.1116/6.0004748","DOIUrl":"10.1116/6.0004748","url":null,"abstract":"Bioactive herbal extracts have garnered significant attention due to their multitarget regulation and low toxicity, yet their clinical applications are limited by poor solubility, low bioavailability, and insufficient targeting. This review systematically summarizes the pharmacological properties of terpenoids, alkaloids, flavonoids, polysaccharides, and other components, and explores their synergistic integration with biomaterials such as nanoparticle delivery systems, microneedles, and hydrogels. Functionalized nanocarriers enhance the stability and targeting efficiency of paclitaxel, berberine, and other bioactive herbal extracts. Microneedle technology leverages physical penetration and sustained-release mechanisms to achieve efficient transdermal delivery of bioactive herbal extracts (e.g., aconitine, curcumin, and similar agents). Smart hydrogels incorporating active molecules (e.g., baicalin and icariin) achieve spatiotemporal precision in wound healing and osteoarthritis treatment through pH-/enzyme-/reactive oxygen species-responsive release mechanisms. Additionally, the combination of herbal extracts with stents or bone cement expands their potential in cardiovascular and bone regeneration applications. While these integrated systems demonstrate synergistic effects in antitumor, anti-inflammatory, and tissue repair, challenges remain in scalable manufacturing, in vivo metabolic mechanisms, and long-term biosafety. Future research should integrate smart biomaterial designs and multiomics analysis to establish a comprehensive \"component-carrier-efficacy\" development framework, advancing the convergence of bioactive herbal extracts and modern medical science.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms underlying the effects of urea and the structural dynamics of bovine serum albumin. 尿素作用的分子机制及牛血清白蛋白的结构动力学。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004688

Yanis R Espinosa, C Manuel Carlevaro, C Gastón Ferrara

引用次数: 0

Competitive adsorption and functional activity of fibrinogen on polyurethane biomaterials surfaces. 纤维蛋白原在聚氨酯生物材料表面的竞争吸附及功能活性。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004598

Li-Chong Xu, Christopher A Siedlecki

{"title":"Competitive adsorption and functional activity of fibrinogen on polyurethane biomaterials surfaces.","authors":"Li-Chong Xu, Christopher A Siedlecki","doi":"10.1116/6.0004598","DOIUrl":"https://doi.org/10.1116/6.0004598","url":null,"abstract":"An immunological atomic force microscopy technique was used to recognize fibrinogen adsorption and functional activity on polyurethane biomaterial surfaces in the presence of other proteins. The amount of fibrinogen adsorbed on surfaces as recognized by an antifibrinogen polyclonal antibody when in competitive adsorption with human serum albumin (HSA) or human IgG was found to be related to the molar ratio of proteins. A significant decrease in fibrinogen adsorption was observed only when the fraction of smaller proteins reached a threshold value, dependent on smaller protein properties. The functional activity of fibrinogen was measured by a monoclonal antibody recognizing a region containing the dodecapeptide sequence located at the C-terminus of the γ-chain, γ-400-411. Results show that the presence of smaller proteins affected the conformational structure of fibrinogen and increased the availability of platelet binding sites in fibrinogen adsorbed on surfaces. Platelet adhesion was performed on polyurethane surfaces, which were competitively preadsorbed with fibrinogen and HSA. Platelet adhesion correlated well with the functional activity of fibrinogen, measured after competitive adsorption on surfaces. The work suggests that platelet adhesion is not necessarily determined by the amount of adsorbed fibrinogen but is related to the activity of fibrinogen as measured by the availability of the platelet binding sites in the fibrinogen, γ-chain dodecapeptide.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chain length of imidazolium ionic liquids on the l-phenylalanine induced phospholipid vesicle membranes: Fluorescence lifetime imaging microscopy study. 咪唑类离子液体链长对l-苯丙氨酸诱导磷脂囊泡膜的影响：荧光寿命成像显微镜研究。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004554

Nanigopal Bera, Sandip Mondal, Arijit Maity, Pratyush Kiran Nandi, Nilmoni Sarkar

引用次数: 0

Bacterial motility in aqueous micro-environment with natural soil particles. 天然土壤颗粒在水微环境中的细菌运动。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004380

Diksha Shrestha, Kishan Mahmud, Sam Mortenson, Mary Savin, Wen Zhang, Yong Wang

{"title":"Bacterial motility in aqueous micro-environment with natural soil particles.","authors":"Diksha Shrestha, Kishan Mahmud, Sam Mortenson, Mary Savin, Wen Zhang, Yong Wang","doi":"10.1116/6.0004380","DOIUrl":"https://doi.org/10.1116/6.0004380","url":null,"abstract":"Bacterial motility is essential for navigating heterogeneous environments like soil, where it plays a key role in nutrient cycling, bioremediation, and overall soil health. Despite its importance, the interplay between bacterial motility and soil microstructures-such as the effects of physical confinement and interfacial interactions-remains underexplored. In this study, we investigated the motility of Escherichia coli bacteria in aqueous micro-environments with three different natural soil samples and examined how the particle size, void fraction, and proximity to soil particles affect bacterial motility and movement patterns by quantitatively analyzing bacterial trajectories, velocities, and directional changes. We observed that bacterial velocity decreased significantly in soil micro-environments, showing a strong positive correlation with the soil particle size and a negative correlation with the void fraction of the soil samples. Additionally, bacteria in soil micro-environments showed rapid and dramatic directional changes, and the rate of directional changes of bacteria was negatively correlated with the particle size. These results were further validated with synthetic micro-environments with glass microspheres. As the density of microspheres increased, the translational velocity of bacteria decreased while the directional changes increased. This study enhances our understanding of how the soil type, porosity, and particle proximity impact bacterial movement and is expected to contribute to a better understanding of bacterial activities on soil health and management.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New way to isolate cultured cell exosomes. 分离培养细胞外泌体的新方法。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004479

İshak Afşin Kariper, Nilgün Okşak, Dilek Bahar

引用次数: 0

Insight into the role of the chemical nature and length of the loop nucleobases in the folding of G-quadruplex by the antimalarial drugs at the DNA interface. 抗疟疾药物在DNA界面上折叠g -四重体时环核碱基的化学性质和长度的作用。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004389

Asim Bisoi, Prashant Chandra Singh

{"title":"Insight into the role of the chemical nature and length of the loop nucleobases in the folding of G-quadruplex by the antimalarial drugs at the DNA interface.","authors":"Asim Bisoi, Prashant Chandra Singh","doi":"10.1116/6.0004389","DOIUrl":"https://doi.org/10.1116/6.0004389","url":null,"abstract":"G-quadruplexes (G4) have been proposed as an alternative target for cancer therapy, as the folding of DNA sequences into stabilized G4 in the cancer microenvironment affects key biological functions. The antimalarial drugs, hydroxychloroquine (HCQ) and chloroquine (CQ), are in the clinical trial stage for cancer therapy and have been found to fold DNA sequences into the stabilized G4 even in the absence of KCl salt. In this study, the role of loop nucleobases in terms of chemical nature, number, and location in the HCQ-/CQ-induced folding of DNA sequences into G4 in the absence of KCl has been investigated systematically. The data indicate that both drugs selectively induce the folding of DNA sequences into G-quadruplexes (G4) that contain thymine loop nucleobases. The folding tendency of DNA sequences into stabilized G4 decreases with the increase in the thymine loop nucleobases. Moreover, DNA sequences with fewer thymine loop nucleobases tend to fold into stable G4 when the thymine residues are present at the terminal positions, whereas sequences with more thymine loop nucleobases show higher G4 folding propensity when these bases are located at the central loop. These findings are important in understanding the anticancer effect of antimalarial drugs.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate viscoelasticity regulates fibroblast adhesion and migration. 底物粘弹性调节成纤维细胞粘附和迁移。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004585

Neha Paddillaya, Akshar Rao, Anshul Shrivastava, Imnatoshi Jamir, Kundan Sengupta, Namrata Gundiah

{"title":"Substrate viscoelasticity regulates fibroblast adhesion and migration.","authors":"Neha Paddillaya, Akshar Rao, Anshul Shrivastava, Imnatoshi Jamir, Kundan Sengupta, Namrata Gundiah","doi":"10.1116/6.0004585","DOIUrl":"10.1116/6.0004585","url":null,"abstract":"Mechanical properties of the extracellular matrix (ECM) modulate cell-substrate interactions and influence cellular behaviors such as contractility, migration, and proliferation. Although the effects of substrate stiffness on mechanobiology have been well studied, the role of ECM viscoelasticity in fibrotic progression remains less understood. To examine how viscoelasticity affects the biophysical properties and regulates signaling of human mammary fibroblasts, we engineered elastic (E) and viscoelastic (VE) polyacrylamide hydrogels with comparable storage moduli (∼14.52 ± 1.03 kPa) but distinctly different loss moduli; mean loss moduli for VE gels was 36.9% higher at 0.05 Hz than E gels. Fibroblasts cultured on E hydrogels spread extensively (2428.93 ± 864.71 μm2), developed prominent stress fibers with higher zyxin intensity, and generated higher traction stresses (2931.57 ± 1732.61 Pa). In contrast, fibroblasts on VE substrates had 54.2% smaller focal adhesion areas, exhibited 51.8% lower critical adhesion strengths, and generated 21% lower traction stresses (p < 0.001). These substrates also promoted migration and showed enhanced proliferation with reduced Yes-associated protein (YAP) activity, suggesting a mechanotransduction shift that may involve alternative signaling pathways. In contrast, E substrates showed YAP nuclear translocation, consistent with greater cytoskeletal tension and contractility. These findings highlight the importance of energy dissipation mechanisms in regulating fibroblast function on substrates mimicking the fibrotic milieu. Our results demonstrate that tuning the ECM viscoelasticity is a useful strategy to regulate cell behaviors in tissue-engineered scaffolds and develop better disease modeling for regenerative medicine.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tryptophan-stabilized gold-manganese oxide nanocomposites for enhanced superoxide dismutase activity. 色氨酸稳定金锰氧化物纳米复合材料增强超氧化物歧化酶活性。

IF 1.9 4区医学

Biointerphases Pub Date : 2025-07-01 DOI: 10.1116/6.0004563

Kanika Bharti, Kalyan K Sadhu

{"title":"Tryptophan-stabilized gold-manganese oxide nanocomposites for enhanced superoxide dismutase activity.","authors":"Kanika Bharti, Kalyan K Sadhu","doi":"10.1116/6.0004563","DOIUrl":"https://doi.org/10.1116/6.0004563","url":null,"abstract":"Syntheses of gold-manganese oxide nanocomposites were attempted by a redox-mediated growth method under varying mild reaction conditions with amino acid as a stabilization agent; finally, the nanocomposites were applied for superoxide dismutase (SOD)-mimic activity. Growth reaction was performed by the reduction of Au(III) with Mn(0) powder on the surface of citrate-stabilized gold nanoparticles as seeds. Variable reaction conditions were attempted to monitor the effect of the pH and, finally, optimized based on the critical properties of the nanocomposites including their long-term stability. In a neutral medium, tryptophan-stabilized Au-Mn3O4 nanocomposites were obtained. Stable Au-Mn2O3 nanocomposites were formed at basic pH in the presence of hydrophobic amino acids. The present work elucidates the role of amino acids, especially tryptophan, in stabilizing gold-manganese oxide nanocomposites. The effect of crystalline vs. the amorphous nature of Mn3O4 sheets in the tryptophan-stabilized nanocomposites was evaluated in SOD-mimetic applications. The IC50 values for the newly synthesized Au-Mn3O4 nanocomposites with crystalline or amorphous Mn3O4 sheets at room temperature were found to be 125 times and 25 times better with respect to the reported Mn3O4 nanoparticles synthesized after calcination at 600 °C. These results provide useful insights into the synthesis of gold-manganese oxide nanocomposites with tunable properties and their potential applications in the growing field of nanozymes.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0