Biointerphases最新文献_第3页

Cellular effects and orientation of immobilized immunoglobulin are correlated to the charge-mediated influence of the antibody variable region. 固定化免疫球蛋白的细胞效应和取向与抗体可变区电荷介导的影响有关。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004169

Hsi-Ju Wei, Jun Zhang, Jeffrey Barbon, Nancy Crosbie, Eric Dominguez

{"title":"Cellular effects and orientation of immobilized immunoglobulin are correlated to the charge-mediated influence of the antibody variable region.","authors":"Hsi-Ju Wei, Jun Zhang, Jeffrey Barbon, Nancy Crosbie, Eric Dominguez","doi":"10.1116/6.0004169","DOIUrl":"10.1116/6.0004169","url":null,"abstract":"Ligand binding to a cell receptor often insufficiently triggers cellular immune responses. Receptor clustering through cross-linking occurs when a ligand binds to two or more receptors, amplifying cellular responses. This is required in certain monoclonal antibodies (mAbs), including effector mechanism activation [binding to fragment crystallizable receptors (FcRs)] or acting as agonists for therapeutic signaling. Therefore, immobilized immunoglobulin immunoassays were developed for efficient diagnostic and therapeutic approaches. The immobilized mAb density and orientation influence the sensitivity and accuracy of these assays. Limited evidence shows that different epitope motifs with the same target mAbs affect immobilized density and orientation in the solid-phase state. Here, we developed a series of fully humanized antidendritic cell immunoreceptor (DCIR) mAbs with different epitopes but the same Fc region. Immobilized anti-DCIR mAbs trigger the effector response from FcR through the Fc region and induce inhibitory pathways from the DCIR intracellular immunoreceptor tyrosine-based inhibitory motif through the fragment variable (Fv) region. In the immobilized immunoglobulin immunoassay, the isoelectric points (pI) of the DCIR mAb Fv region, not the total pI, significantly correlate to the surface density and orientation of immobilized mAbs on negatively charged plates. Cytokine production and protein phosphorylation in human monocytes were affected by vary binding abilities of immobilized mAbs to the plate. Methods, such as increasing hydrophobicity or ionic interactions, have improved the surface density and consistent orientation of immobilized anti-DCIR mAbs. Our study highlights the critical relationship between the net charge of the antibody Fv region and its immobilization potential in the solid-phase state.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors behind the spontaneous synthesis of spherical brushite from wheat bran aqueous extract: The role of zinc. 麦麸水提物自发合成球形刷石的影响因素：锌的作用。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004184

Iván Jalil Antón Carreño-Márquez, René Renato Balandrán-Quintana, José Antonio Azamar-Barrios, Ana María Mendoza-Wilson, Gabriela Ramos-Clamont Montfort, Daniela Denisse Castro-Enríquez

{"title":"Factors behind the spontaneous synthesis of spherical brushite from wheat bran aqueous extract: The role of zinc.","authors":"Iván Jalil Antón Carreño-Márquez, René Renato Balandrán-Quintana, José Antonio Azamar-Barrios, Ana María Mendoza-Wilson, Gabriela Ramos-Clamont Montfort, Daniela Denisse Castro-Enríquez","doi":"10.1116/6.0004184","DOIUrl":"10.1116/6.0004184","url":null,"abstract":"Coprecipitation biomineralization was induced using nondialyzed and dialyzed aqueous wheat bran extracts as scaffolds, to which zinc (Zn) was added in a 0%-15% concentration range. Spherical particles of brushite were precipitated up to 3% Zn concentration in the nondialyzed extracts. At 5% and 10% Zn, spherical or spheroidal brushite particles were precipitated, but the internal microstructure changed from stacked plates to laid parallel strands; a secondary weddellite phase was formed. Brushite with 0.018% Zn content was formed even without external additions due to the natural presence of Zn in the nondialyzed extracts. The Zn content of doped brushite particles was between 0.74% and 1% by weight for the 3%-10% added Zn range. Higher concentrations of Zn inhibited crystal growth. In dialyzed extracts, brushite spherical particles were formed only without added external Zn. However, crystal morphology was very similar, and the radial arrangement was maintained. Amorphous material with varied elemental composition precipitated only when Zn was added to the dialyzed extracts. Lattice parameters of brushite were close to those found in the literature, with minor variations for b and c. The results show the evidence of the role of Zn in the spherical morphology of brushite.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of sequence length and functionalization in interactions of bioconjugated peptides with mitomembranes. 序列长度和功能化在生物偶联肽与有丝分裂膜相互作用中的作用。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004197

Son V Nguyen, Roy P Planalp, Harish Vashisth

{"title":"Role of sequence length and functionalization in interactions of bioconjugated peptides with mitomembranes.","authors":"Son V Nguyen, Roy P Planalp, Harish Vashisth","doi":"10.1116/6.0004197","DOIUrl":"10.1116/6.0004197","url":null,"abstract":"Cell-penetrating peptides are efficient tools for intracellular delivery of a variety of cargoes. In this study, we explored the effect of chain length, side chain chemistry, and the locations of conjugated molecules on the interaction between iron-chelating peptides and a mitochondrial-mimicking membrane. We report that a longer chain length enhanced peptide/membrane interactions, and conjugation at the N-terminus lowered the free-energy barrier for peptide translocation across the membrane. Peptides containing Phe side chains and those containing modified Phe (cyclohexane) side chains showed comparable peptide/membrane energetics and translocation energy barriers. Using steered molecular dynamics (SMD) simulations, we further probed the mechanistic details of translocation of each N-terminated peptide across the membrane and compared their metastable states. At a higher steering velocity, the peptide adopted a compact structure due to frequent π-π interactions among conjugated molecules, but at lower steering velocities, each N-terminated peptide adopted an extended structure. This structure allowed cationic residues to maximize their interactions with phosphate headgroups in the mitomembrane. The hydrophobic residues also formed interactions with the lipid acyl tails, facilitating the passage of peptides across the membrane with decreased free energy barriers. Our results highlight the significance of peptide chain length and conjugation in facilitating peptide transport across the membrane.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of morphology and surface properties on the antibacterial action of GaOOH microparticles. 形态和表面性质对高ooh微粒抗菌作用的影响。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0004133

D A Johnson, P Ahluwalia, J H Brannon, P Jodhka, V Wayman, Z Rabine, Y M Strzhemechny

引用次数: 0

Temporal dynamics of the biofilm-mediated open circuit potentials: Understanding the fundamentals via a combined thermodynamic and kinetic modeling approach. 生物膜介导的开路电位的时间动力学：通过结合热力学和动力学建模方法来理解基本原理。

IF 1.6 4区医学

Biointerphases Pub Date : 2025-01-01 DOI: 10.1116/6.0003996

Ljupcho Pejov, Kiril D Hristovski, Scott R Burge, Russell G Burge, Dragan Boscovic

{"title":"Temporal dynamics of the biofilm-mediated open circuit potentials: Understanding the fundamentals via a combined thermodynamic and kinetic modeling approach.","authors":"Ljupcho Pejov, Kiril D Hristovski, Scott R Burge, Russell G Burge, Dragan Boscovic","doi":"10.1116/6.0003996","DOIUrl":"10.1116/6.0003996","url":null,"abstract":"This study provides in-depth insights into the thermodynamics of electrochemical processes that govern the generation and temporal modulation of open-circuit potentials in biofilms and presents the foundation and applications of open-circuit potential methods to study the bioelectrochemical behaviors of biofilms. This investigation was guided by an overarching hypothesis that models should adequately explain the open-circuit potential patterns generated by biofilms when environmental conditions change; and from this work, a generalized model of electrochemical processes endemic to the biofilm electrode was developed and validated. The proposed model accounts for open system thermodynamics and the kinetics of bioelectrochemical transformations, and the model is simplified to enable applicability to a wide range of processes that are possible within biofilms. As such, the model can account for different parameters associated with various biofilm systems and is extendable to include numerous other experimental conditions. The model predictions were compared to the experimental data generated by 48 equidistantly located microbial potentiometric sensor electrodes in a chamber capable of simulating naturally occurring water matrix, which was exposed to environmental conditions. By combining electrochemical-cell thermodynamics and kinetics approaches, the model explained the temporal dependences of the open circuit potentials in aerobic and anaerobic conditions and the interconversion of two regimes commonly observed in natural systems. At the same time, it enables extraction of the relevant kinetic parameters from experimentally measured time evolution of the open circuit potentials.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"20 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interfacial crack self-healing by Sporosarcina pasteurii: From medium optimization to spore encapsulation. 巴氏孢杆菌界面裂缝自愈：从培养基优化到孢子包封。

IF 1.6 4区医学

Biointerphases Pub Date : 2024-11-01 DOI: 10.1116/6.0004099

Chenxi Hou, Chu Wang, Ling Zheng, Jie Peng, Tao Yuan, Hui Huang, Xiaolin Lu

{"title":"Interfacial crack self-healing by Sporosarcina pasteurii: From medium optimization to spore encapsulation.","authors":"Chenxi Hou, Chu Wang, Ling Zheng, Jie Peng, Tao Yuan, Hui Huang, Xiaolin Lu","doi":"10.1116/6.0004099","DOIUrl":"https://doi.org/10.1116/6.0004099","url":null,"abstract":"Self-healing cement takes advantage of microbial induced carbonate precipitation (MICP), a meritorious biological process, to achieve automatic healing of cement cracks. In this study, two beneficial factors, optimization of the bacteria culture medium and encapsulation of bacterial spores, were used to improve the MICP efficiency of Sporosarcina pasteurii in self-healing cement. On the one hand, in medium optimization, we compared the growth of Sporosarcina pasteurii fed with two generally used nitrogen sources, e.g., urea and ammonium chloride, and found that ammonium chloride can promote biomineralization more efficiently than urea. It was also confirmed that nickel (0.1 mg/l) and manganese ions (10 mg/l) benefit the MICP process through enhancement of urease activity and promotion of spore production. On the other hand, spores encapsulated in sodium alginate-gelatin gel beads prepared by using a flow nozzle device can have excellent swelling performance triggered by water. As an application demonstration, self-healing of cement cracks with consideration of the above beneficial factors was successfully verified without substantial influence on the cement compressive strength.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 6","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular-level studies of extracellular matrix proteins conducted using atomic force microscopy. 使用原子力显微镜对细胞外基质蛋白质进行分子水平的研究。

IF 2.1 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003789

Ashley R Walker,Jonathan R Sloneker,Jayne C Garno

{"title":"Molecular-level studies of extracellular matrix proteins conducted using atomic force microscopy.","authors":"Ashley R Walker,Jonathan R Sloneker,Jayne C Garno","doi":"10.1116/6.0003789","DOIUrl":"https://doi.org/10.1116/6.0003789","url":null,"abstract":"Extracellular matrix (ECM) proteins provide anchorage and structural strength to cells and tissues in the body and, thus, are fundamental molecular components for processes of cell proliferation, growth, and function. Atomic force microscopy (AFM) has increasingly become a valuable approach for studying biological molecules such as ECM proteins at the level of individual molecules. Operational modes of AFM can be used to acquire the measurements of the physical, electronic, and mechanical properties of samples, as well as for viewing the intricate details of the surface chemistry of samples. Investigations of the morphology and properties of biomolecules at the nanoscale can be useful for understanding the interactions between ECM proteins and biological molecules such as cells, DNA, and other proteins. Methods for preparing protein samples for AFM studies require only basic steps, such as the immersion of a substrate in a dilute solution or protein, or the deposition of liquid droplets of protein suspensions on a flat, clean surface. Protocols of nanolithography have been used to define the arrangement of proteins for AFM studies. Using AFM, mechanical and force measurements with tips that are coated with ECM proteins can be captured in ambient or aqueous environments. In this review, representative examples of AFM studies are described for molecular-level investigations of the structure, surface assembly, protein-cell interactions, and mechanical properties of ECM proteins (collagen, elastin, fibronectin, and laminin). Methods used for sample preparation as well as characterization with modes of AFM will be discussed.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"33 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of metal oxides on biocompatibility of additively manufactured NiTi. 金属氧化物对加成制造镍钛生物相容性的影响

IF 1.6 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003665

Maria P Kwesiga, Roger J Guillory, Ali Gökhan Demir

{"title":"Influence of metal oxides on biocompatibility of additively manufactured NiTi.","authors":"Maria P Kwesiga, Roger J Guillory, Ali Gökhan Demir","doi":"10.1116/6.0003665","DOIUrl":"https://doi.org/10.1116/6.0003665","url":null,"abstract":"In order to properly satisfy biomedical constraints for cardiovascular applications, additively manufactured NiTi scaffolds required further process and metallurgical engineering. Additively manufactured NiTi materials for cardiovascular use will have to undergo surface finishing in order to minimize negative surface interactions within the artery. In this study, we sought to understand biocompatibility from chemically etched additively manufactured NiTi scaffolds by laser powder bed fusion (LPBF). Although two distinct oxide films were created in the surface etching process (labeled CP-A and CP-B), no qualitative changes in microroughness were seen between the two conditions. CP-A possessed significantly less Ni at the surface (0.19 at. %) than the CP-B group (3.30 at. %), via x-ray photoelectron spectroscopy, alongside a concomitant shift in the O1 s peak presentation alluding to a greater formation of a Ni based oxide in the CP-B group. Our live dead staining revealed significant toxicity and reduced cellular attachment for the CP-B group, in addition to inducing more cell lysis (20.9 ± 5.1%), which was significantly increased when compared to CP-A (P < 0.01). Future practices of manufacturing NiTi scaffolds using LPBF should focus on producing surface films that are not only smooth, but free of cytotoxic Ni based oxides.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying microbially induced corrosion on glass using ToF-SIMS. 利用 ToF-SIMS 研究微生物诱发的玻璃腐蚀。

IF 1.6 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003883

Gabriel D Parker, Andrew Plymale, Jacqueline Hager, Luke Hanley, Xiao-Ying Yu

{"title":"Studying microbially induced corrosion on glass using ToF-SIMS.","authors":"Gabriel D Parker, Andrew Plymale, Jacqueline Hager, Luke Hanley, Xiao-Ying Yu","doi":"10.1116/6.0003883","DOIUrl":"https://doi.org/10.1116/6.0003883","url":null,"abstract":"Microbially induced corrosion (MIC) is an emerging topic that has huge environmental impacts, such as long-term evaluation of microbial interactions with radioactive waste glass, environmental cleanup and disposal of radioactive material, and weathering effects of microbes. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), a powerful mass spectral imaging technique with high surface sensitivity, mass resolution, and mass accuracy, can be used to study biofilm effects on different substrates. Understanding how to prepare biofilms on MIC susceptible substrates is critical for proper analysis via ToF-SIMS. We present here a step-by-step protocol for preparing bacterial biofilms for ToF-SIMS analysis, comparing three biofilm preparation techniques: no desalination, centrifugal spinning (CS), and water submersion (WS). Comparisons of two desalinating methods, CS and WS, show a decrease in the media peaks up to 99% using CS and 55% using WS, respectively. Proper desalination methods also can increase biological signals by over four times for fatty acids using WS, for example. ToF-SIMS spectral results show chemical compositional changes of the glass exposed in a Paenibacillus polymyxa SCE2 biofilm, indicating its capability to probe microbiologically induced corrosion of solid surfaces. This represents the proper desalination technique to use without significantly altering biofilm structure and substrate for ToF-SIMS analysis. ToF-SIMS spectral results showed chemical compositional changes of the glass exposed by a Paenibacillus bacterial biofilm over 3-month inoculation. Possible MIC products include various phosphate phase molecules not observed in any control samples with the highest percent increases when experimental samples were compared with biofilm control samples.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenomenological investigation of organic modified cements as biocompatible substrates interfacing model marine organisms. 有机改性水泥作为生物相容性基质与海洋模式生物界面的现象学研究。

IF 1.6 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003811

Jinglun Zhao, Tao Yuan, Hui Huang, Xiaolin Lu

引用次数: 0