Bioprocess and Biosystems Engineering最新文献_第7页

Thinking big in small scale: characterization of peroxidase production in Komagataella phaffii using two-compartment scale-down system. 在小范围内思考大：利用双室缩小系统表征Komagataella phaffii过氧化物酶的生产。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-09-01 Epub Date: 2026-08-28 DOI: 10.1007/s00449-026-03415-6

Christian Mathis Wagner, Katharina Schwartz, Anton Glieder, Wolfgang Wiechert, Marco Oldiges

{"title":"Thinking big in small scale: characterization of peroxidase production in Komagataella phaffii using two-compartment scale-down system.","authors":"Christian Mathis Wagner, Katharina Schwartz, Anton Glieder, Wolfgang Wiechert, Marco Oldiges","doi":"10.1007/s00449-026-03415-6","DOIUrl":"10.1007/s00449-026-03415-6","url":null,"abstract":"The replacement of cobalt-based siccatives in unsaturated polyester resin curing requires sustainable and scalable biocatalytic alternatives, with fungal peroxidases representing a promising option. For industrial implementation, however, robust large-scale production processes are essential. In this study, we investigated the robustness of a glycerol-based peroxidase production process in K. phaffii driven by the glycerol-derepressible PDF promoter, focusing on industrially relevant bioreactor inhomogeneities. A two-compartment scale-down system was used to mimic substrate and oxygen gradients arising from insufficient mixing and mass transfer at large scale. Substrate inhomogeneity alone led to a moderate reduction in peroxidase titers (approx. 23%), indicating partial sensitivity to localized substrate oversupply. In contrast, simultaneous substrate and oxygen inhomogeneity resulted in a counterintuitive increase in peroxidase titers of approx. 13% compared to homogeneous reference conditions. This suggests that dynamic transitions between substrate limitation and surplus can positively affect peroxidase formation under PDF promoter control. Based on this observation, a modified process strategy incorporating glycerol pulses during the depression phase was evaluated. While this approach demonstrated high robustness, it did not enhance productivity. Overall, the results demonstrate that the developed K. phaffii process is resilient to large-scale inhomogeneities and can even benefit from specific dynamic cultivation conditions.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2429-2440"},"PeriodicalIF":4.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elicitors derived from endophytic fungus Acremonium sp. enhance triterpenoid and polysaccharide production in the submerged cultivation of the medicinal mushroom Inonotus obliquus. 从内生真菌Acremonium sp.提取的激发子提高了药用菌Inonotus obliquus潜水培养中三萜和多糖的产量。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-02 DOI: 10.1007/s00449-026-03378-8

Ming Yang, Yaqi Shi, Yong Sun, Jiangsheng Zhou, Jihong Jiang

{"title":"Elicitors derived from endophytic fungus Acremonium sp. enhance triterpenoid and polysaccharide production in the submerged cultivation of the medicinal mushroom Inonotus obliquus.","authors":"Ming Yang, Yaqi Shi, Yong Sun, Jiangsheng Zhou, Jihong Jiang","doi":"10.1007/s00449-026-03378-8","DOIUrl":"10.1007/s00449-026-03378-8","url":null,"abstract":"Triterpenoids and polysaccharides produced by Inonotus obliquus exhibit antitumor, antioxidant and hypoglycemic activities. Increasing the yields of triterpenoids and polysaccharides could effectively enhance the medicinal value of I. obliquus. In this study, the feasibility of improving the production of triterpenoids and polysaccharides by adding elicitors including cell wall debris (CW) and culture filtrate (CF) derived from endophytic fungus Acremonium sp. in the submerged cultivation of I. obliquus was investigated. The optimal concentrations and times for the addition of CW and CF were determined to be 60 µg/mL on day 3 and 10% v/v on day 1, respectively. The maximum total triterpenoid production of CW and CF groups increased by 19.38% and 49.81%, respectively, compared to the control. The maximum intracellular polysaccharide (IPS) production obtained with the CW and CF were 3.97 ± 0.24 and 4.95 ± 0.40 g/L, representing significant increases of 17.70% and 46.85% compared to the control. Additionally, the production of extracellular polysaccharides (EPS) was enhanced by 27.23% and 45.35% with the CW and CF, respectively, in comparison to the control. Furthermore, the transcription levels of the key genes involved in the biosynthesis of triterpenoids and polysaccharides were significantly upregulated in response to the elicitors. This study provides a novel approach for enhancing the bioactive metabolites production of medicinal basidiomycota by the elicitors derived from endophytes.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2119-2131"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Techno-economic evaluation of Islamic-compliant cell-based influenza vaccine production using AGE1.CR cell line in comparison to the current production using MDCK cell line. 使用AGE1生产符合伊斯兰教的细胞流感疫苗的技术经济评价。CR细胞系与目前生产使用的MDCK细胞系比较。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-09 DOI: 10.1007/s00449-026-03377-9

Nurul Nadiah Zulkarnain, Nurina Anuar, Norliza Abd Rahman, Muhammad Nazir Alias, Peer Mohamed Abdul, Nazahiyah Ahmad Rodzli, Siti Rozaimah Sheikh Abdullah

{"title":"Techno-economic evaluation of Islamic-compliant cell-based influenza vaccine production using AGE1.CR cell line in comparison to the current production using MDCK cell line.","authors":"Nurul Nadiah Zulkarnain, Nurina Anuar, Norliza Abd Rahman, Muhammad Nazir Alias, Peer Mohamed Abdul, Nazahiyah Ahmad Rodzli, Siti Rozaimah Sheikh Abdullah","doi":"10.1007/s00449-026-03377-9","DOIUrl":"10.1007/s00449-026-03377-9","url":null,"abstract":"Influenza vaccination is administered annually to prevent and reduce morbidity and mortality associated with highly mutable influenza viruses that can cause severe respiratory disease and unpredictable pandemics. In response to limitations of egg-based production, influenza vaccine manufacturing has increasingly shifted toward cell-based platforms. However, current cell-based influenza vaccines rely on materials like MDCK cells that being considered non-permissible from an Islamic perspective which potentially contributing to vaccine hesitancy among Muslim populations. This study evaluates the techno-economic feasibility of the Islamic-compliant cell-based influenza vaccine production process incorporating alternative permissible inputs including AGE1.CR cell line. Process modelling and economic analysis were performed using SuperPro Designer® to assess productivity and economic performance indicators. The results demonstrate that the proposed Islamic-compliant process achieves productivity levels comparable to the current cell-based systems and meets standard economic feasibility criteria. However, the estimated unit selling price is approximately three-fold higher than the current market price, reflecting higher production costs associated with the compliant process configuration. These findings indicate that the Islamic-compliant cell-based influenza vaccine production is technically feasible and economically viable under premium pricing conditions. Further experimental validation and pilot-scale studies are required to substantiate the simulation outcomes and to identify opportunities for cost reduction prior to large-scale implementation.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2101-2117"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards scalable production of recombinant EIT as a protective antigen against EHEC. 面向大规模生产重组EIT作为肠出血性大肠杆菌的保护抗原。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-22 DOI: 10.1007/s00449-026-03398-4

Laura A Basile, Diego G Noseda, Ingrid Milstein, Mara S Roset, Gabriel Briones

{"title":"Towards scalable production of recombinant EIT as a protective antigen against EHEC.","authors":"Laura A Basile, Diego G Noseda, Ingrid Milstein, Mara S Roset, Gabriel Briones","doi":"10.1007/s00449-026-03398-4","DOIUrl":"10.1007/s00449-026-03398-4","url":null,"abstract":"Cattle are the major reservoir and an important source of environmental dissemination of enterohemorrhagic E. coli (EHEC), a human pathogen responsible for outbreaks of bloody diarrhea and hemolytic uremic syndrome (HUS) worldwide. Preharvest vaccines are aimed to reduce bacterial carriage and consequently, the impact of this zoonosis. The chimeric antigen EIT has demonstrated to be protective against EHEC in murine models. In a recent study, we proposed a simple and cost-effective approach for a vaccine formulation for cattle, in which EIT was expressed and recovered from the periplasm of the laboratory strain E. coli BL21. As a proof-of-concept, the protective efficacy in mice as well as the induction of humoral immune responses in bovines were verified. In this work, we aimed to improve the production of the recombinant antigen EIT using E. coli fed-batch fermentations. An initial screening was conducted to select the medium that best sustained EIT expression using lactose for heterologous induction. M9 medium supplemented with yeast extract yielded the highest relative levels of EIT and was used for subsequent fermentation in a stirred-tank bioreactor. Thus, EIT concentration and volumetric productivity increased by approximately 600% compared to shake-flask cultivations, while EIT yield relative to biomass and specific productivity increased by approximately 130%. Following thermal permeabilization of cells for antigen recovery, the method yielded, overall, about 350 doses per L of fermented culture. The process was reproducible across three independent fermentations while retaining EIT antigenicity, as assessed by ELISA, and functionality, as it could induce antibodies that inhibited actin pedestal formation in in vitro infection assays. Thus, heterologous EIT expression was successfully transferred to a stirred-tank bioreactor, increasing antigen output and establishing a basis for further development of a scalable process for EHEC vaccine applications.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2291-2301"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148547607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox-centric metabolic rewiring for dark-fermentative hydrogen production in Enterobacter aerogenes. 产气肠杆菌暗发酵产氢的氧化还原中心代谢重布线。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-07 DOI: 10.1007/s00449-026-03376-w

Gengran Zhai, Qingyao Jiang, Yilin Ding, Ruoxuan Bai, Jiale Chen, Fangxu Xu, Hongxin Zhao, Hongwei Fu

{"title":"Redox-centric metabolic rewiring for dark-fermentative hydrogen production in Enterobacter aerogenes.","authors":"Gengran Zhai, Qingyao Jiang, Yilin Ding, Ruoxuan Bai, Jiale Chen, Fangxu Xu, Hongxin Zhao, Hongwei Fu","doi":"10.1007/s00449-026-03376-w","DOIUrl":"10.1007/s00449-026-03376-w","url":null,"abstract":"Dark-fermentative hydrogen is promising but constrained by acidification, mixed-acid by-products, and competition for reducing equivalents. Enterobacter aerogenes IAM1183, a rapidly growing and substrate-flexible facultative anaerobe, was used as the H2-producing chassis. A unified benchmark was then established to systematically redirect flux along three levers: deleting the pyruvate formate-lyase-associated pflAB locus (ΔpflAB), enlarging reducing power/electron transfer by overexpressing pntA or fdx, and deleting phosphoenolpyruvate carboxylase ppc (Δppc) to probe pyruvate-node pressure. In 20 h glucose fermentations, H2 yield increased by 38.0% in Ea/pntA, 32.8% in Ea/fdx, and 31.3% in ΔpflAB, whereas Δppc lowered H2 yield by 10.5%. Intracellular NADH/NAD+ increased by 137% in Ea/pntA and by approximately 51% in ΔpflAB. In ΔpflAB, formate fell below HPLC detection, endpoint pH was 6.06 versus 4.70 in WT, and final biomass increased by approximately 50%, consistent with a strong apparent shift away from detectable formate-associated H2 production under the present batch condition. Shifts toward acetoin and 2,3-butanediol across engineered strains indicate residual NADH sinks accompanying redox gains. Together, these side-by-side data distill actionable rules: alleviate acid load, expand reducing capacity, and constrain NADH sinks. They also nominate ΔpflAB combined with pntA or fdx overexpression as a promising design for future validation under pH-controlled and gas-removal conditions. This integrated evaluation clarifies how de-acidification, redox reinforcement, and carbon redistribution jointly reshape endpoint H2 phenotypes in E. aerogenes, providing a coherent platform for future strain-and-process co-optimization of dark-fermentative biohydrogen production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2085-2100"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-yield soluble expression and affinity-tag-free purification of growth hormone receptor antagonist B2036 in Escherichia coli. 生长激素受体拮抗剂B2036在大肠杆菌中的高效可溶性表达及无亲和标签纯化。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-17 DOI: 10.1007/s00449-026-03388-6

Haoju Hua, Yapeng Wang, Junteng Feng, Yinuo Feng, Ying Zhang, Jianguang Lu, Jun Feng

{"title":"High-yield soluble expression and affinity-tag-free purification of growth hormone receptor antagonist B2036 in Escherichia coli.","authors":"Haoju Hua, Yapeng Wang, Junteng Feng, Yinuo Feng, Ying Zhang, Jianguang Lu, Jun Feng","doi":"10.1007/s00449-026-03388-6","DOIUrl":"10.1007/s00449-026-03388-6","url":null,"abstract":"Acromegaly is a severe endocrine disease, and growth hormone receptor antagonist B2036 constitutes the core protein backbone of pegvisomant. Conventional B2036 production based on inclusion bodies or periplasmic expression suffers from low yield, complicated refolding, and multi-step purification. Herein, we developed a highly efficient, refolding-free bioprocess for soluble expression of B2036 in Escherichia coli using SUMO fusion technology. The entire fermentation cycle was controlled within 24 h, and fermentation optimization via response surface methodology enabled a high-yield soluble yield of 0.95 g/L. For downstream processing, an industrial-scalable, affinity-tag-free strategy using only two chromatography steps (anion-exchange and mixed-mode chromatography) was established, producing 498.47 mg/L B2036 with 97.12% purity, representing a 20-fold improvement over previous reports. The obtained B2036 showed correct sequence, precise disulfide pairing, and strong biological activity with an IC₅₀ of 0.67 nmol/L. By removing protein refolding and metal-affinity chromatography, this simplified and robust workflow remarkably improves productivity and product quality, offering a cost-effective and scalable technical platform for large-scale manufacturing of pegvisomant biosimilars. Collectively, these findings provide a valuable reference for developing efficient soluble expression and downstream purification processes of other intrachain disulfide-bonded proteins in E. coli.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2225-2237"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application potential of a marine-derived halotolerant yeast in saline azo dye wastewater treatment: elucidating degradation pathways and osmo-adaptive strategies. 海洋耐盐酵母在含盐偶氮染料废水处理中的应用潜力：阐明降解途径和渗透适应策略。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-20 DOI: 10.1007/s00449-026-03396-6

Zhiyu Dong, Haiyun Zhang, Bingwen Xu, Xinmei Fu, Liang Tan

{"title":"Application potential of a marine-derived halotolerant yeast in saline azo dye wastewater treatment: elucidating degradation pathways and osmo-adaptive strategies.","authors":"Zhiyu Dong, Haiyun Zhang, Bingwen Xu, Xinmei Fu, Liang Tan","doi":"10.1007/s00449-026-03396-6","DOIUrl":"10.1007/s00449-026-03396-6","url":null,"abstract":"A halotolerant yeast strain designated ZY was isolated from marine sediment and identified as Candida tropicalis, in the present study. The strain demonstrated efficient decolorization of multiple azo dyes under high-salinity conditions, with NaCl concentrations ranging from 10.0 to 110.0 g/L. Through single-factor optimization, the growing cells of the yeast ZY achieved over 97% removal of the model azo dye Acid Red 3R (1600.0 µmol/L, approximately equivalent to 861.6 mg/L) within 12 h under the optimized conditions [sucrose 2.0 g/L, (NH4)2SO4 0.2 g/L, yeast extract 0.06 g/L, NaCl 30.0 g/L, 160 rpm, 35 °C, pH 6.0]. Acute toxicity assays indicated significant detoxification of the dye 3R after biodegradation. Activities of key enzymes, including NADH-DCIP reductase, lignin peroxidase, manganese peroxidase and laccase, were determined intracellularly. Metabolites and transcriptomics analysis revealed a proposed degradation pathway involving azo bond cleavage, hydroxylation, deamination, desulfurization, ring opening and ultimately mineralization via the TCA cycle. Comparative transcriptomics further revealed that the yeast ZY adapts to high salinity through cell wall remodeling, ion homeostasis, compatible solute accumulation and stress-responsive gene regulation. This study highlights the potential of the yeast ZY as a promising biocatalyst for treating saline azo dye wastewater.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2271-2290"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial fuel cell-assisted constructed wetlands for wastewater treatment and energy recovery: advances, performance trends, and future directions. 微生物燃料电池辅助人工湿地用于废水处理和能量回收：进展、性能趋势和未来方向。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-21 DOI: 10.1007/s00449-026-03391-x

Nidhi Ahlawat, Vishal Panghal, Pooja Choudhary, Annu Radha Nandal, Sandeep Kumar, Arup Giri, Sunil Kumar

{"title":"Microbial fuel cell-assisted constructed wetlands for wastewater treatment and energy recovery: advances, performance trends, and future directions.","authors":"Nidhi Ahlawat, Vishal Panghal, Pooja Choudhary, Annu Radha Nandal, Sandeep Kumar, Arup Giri, Sunil Kumar","doi":"10.1007/s00449-026-03391-x","DOIUrl":"10.1007/s00449-026-03391-x","url":null,"abstract":"The accelerating degradation of global water resources and the growing energy insecurity have intensified the demand for sustainable technologies that can simultaneously treat wastewater and generate energy. Constructed wetlands integrated with microbial fuel cells (CW-MFCs) have emerged as an innovative nature-based solution that synergistically combines ecological treatment processes with bioelectrochemical energy generation. This review summarizes recent advancements in CW-MFC technology, focusing on system mechanisms, structural configurations, operational parameters, and performance outcomes. A comprehensive bibliometric analysis was conducted to identify emerging trends, key developments, and knowledge gaps in CW-MFC research. A detailed comparative assessment of representative studies highlights the influence of wetland vegetation, electrode materials, microbial communities, pollutant removal efficiencies, and metrics related to bioelectricity generation. Multivariate bubble plots and treemap visualizations are employed to systematically demonstrate how variations in plant species, electrode configurations, and microbial assemblages affect chemical oxygen demand (COD) removal (60-95%) and voltage outputs (300-800 mV). Beyond conventional organic matter and nutrient removal, the applicability of CW-MFCs for treating emerging contaminants, including antibiotics, dyes, and heavy metals, is critically evaluated. Key technical, operational, and scalability challenges restricting large-scale deployment are identified, along with potential strategies for system optimization. CW-MFCs improve water quality by removing pollutants, provide a sustainable sanitation solution, and enable clean energy generation while reducing greenhouse gas emissions. These abilities of CW-MFC align with SDG 6 (Clean Water and Sanitation), 7 (Affordable and Clean Energy), and 13 (Climate Action).","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1993-2031"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate. 以废食用油为底物的PHA混合微生物培养的富集。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-09 DOI: 10.1007/s00449-026-03359-x

C Ucha, D Correa-Galeote, A Val Del Rio, A Pedrouso, A Mosquera-Corral

{"title":"Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate.","authors":"C Ucha, D Correa-Galeote, A Val Del Rio, A Pedrouso, A Mosquera-Corral","doi":"10.1007/s00449-026-03359-x","DOIUrl":"10.1007/s00449-026-03359-x","url":null,"abstract":"The synthesis of polyhydroxyalkanoates (PHA) by a mixed microbial culture (MMC) employing used cooking oil (UCO) as substrate represents a promising bioprocess for organic wastes valorisation. In this study, MMC enrichment in a sequencing batch reactor (SBR) was investigated using UCO without pretreatment as the sole carbon source. Two SBRs (SBR1 and SBR2) were operated under a feast-famine regime with uncoupled carbon and nitrogen supply, differing in the timing of the biomass withdrawal and nutrient filling. In SBR1, with effluent withdrawal at the end of the feast phase, better poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) accumulation was achieved (average values of 4.2 ± 1.6 wt. %, maximum of 19.9 wt. %) compared to SBR2, with withdrawal at the end of the famine phase (average values of 2.7 ± 1.1 wt. %, maximum of 5.2 wt. %). The highest PHBV accumulation in SBR1 was obtained at a COD/N ratio of 46 g/g, with an active biomass concentration of approximately 1 g VSS/L. Additionally, a single UCO pulse enhanced substrate hydrolysis and consumption compared to multiple pulses. Microbial community analysis revealed the enrichment of bacterial OTUs with PHA accumulation capacity related to Azospirillum, Acinetobacter, Gordonia, Burkholderia, and Bacillus. Among fungal OTUs, Geotrichum, Meyerozyma, and Pascua were the most abundant. Multivariate statistical analysis indicated that the synergistic interaction of Burkholderia and Chitinophaga played a crucial role in achieving the highest PHBV accumulation levels.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2045-2059"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13424269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of three inoculum sources for acetate production and microbial succession in H₂/CO₂-fed anaerobic system. 三种接种源对H2/ co2厌氧系统中乙酸生成及微生物演替的比较。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-02 DOI: 10.1007/s00449-026-03381-z

Nana Jiang, Linxiao Li, Xiyan Li, Ruixiang Li, Guoliang Wang, Tian Li, Xin Wang

{"title":"Comparison of three inoculum sources for acetate production and microbial succession in H2/CO2-fed anaerobic system.","authors":"Nana Jiang, Linxiao Li, Xiyan Li, Ruixiang Li, Guoliang Wang, Tian Li, Xin Wang","doi":"10.1007/s00449-026-03381-z","DOIUrl":"10.1007/s00449-026-03381-z","url":null,"abstract":"Introducing a synthetic H2/CO2 gas mixture into anaerobic systems is a promising strategy for C1 gas valorization, renewable energy storage, and sustainable chemical synthesis. However, systematic comparisons of inoculum sources under identical operational conditions remain limited. In this study, three inoculum sources, fermentation sludge (S1), lake sediment (S2), and brewery anaerobic sludge (S3), were compared for acetate production from CO2 and H2, with particular attention to microbial community succession and reactor stability. S3 showed the highest CO2 consumption rate (331 ± 4 mg CO2/(L·d)), cumulative CO2 fixation (7.8 ± 0.3 g/L), and acetate production efficiency (5.3% electron allocation to acetate), along with stable pH conditions (7.1 ± 0.3). These outcomes were associated with the rapid enrichment of genera that include known homoacetogenic species, mainly Clostridium and Acetobacterium, which together reached approximately 40% relative abundance in S3 after acclimation. The findings suggest that initial inoculum composition can influence the extent of homoacetogen enrichment and subsequent acetate production performance. In addition, the post-peak increase in the acetate-oxidizing genus Geobacter (an acetate consumer rather than an acetogen) in S3 points to a potential challenge for sustained product recovery in mixed-culture systems. Overall, these results provide insights for inoculum selection and process optimization in H2/CO2 fermentation systems for acetate production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2133-2145"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0