Bioprocess and Biosystems Engineering最新文献_第7页

Improved catalytic stability of immobilized Candida antarctica lipase B on macroporous resin with organic polymer coating for biodiesel production. 提高大孔树脂上固定化白色念珠菌脂肪酶 B 的催化稳定性，使其具有有机聚合物涂层，用于生物柴油生产。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-10-22 DOI: 10.1007/s00449-024-03099-w

Jiale Liu, Shufan Zhao, Wan Wei, Shupeng Yu, Zhao Wang, Jianyong Zheng

{"title":"Improved catalytic stability of immobilized Candida antarctica lipase B on macroporous resin with organic polymer coating for biodiesel production.","authors":"Jiale Liu, Shufan Zhao, Wan Wei, Shupeng Yu, Zhao Wang, Jianyong Zheng","doi":"10.1007/s00449-024-03099-w","DOIUrl":"10.1007/s00449-024-03099-w","url":null,"abstract":"Lipase is one of the most widely studied and applied biocatalysts. Due to the high enzyme leakage rate of the immobilization method of physical adsorption, we propose a new lipase immobilization method, based on the combination of macroporous resin adsorption and organic polymer coating. The immobilized Candida antarctica lipase B (CALB@resin-CAB) was prepared by combining the macroporous resin adsorption with cellulose acetate butyrate coating, and its structure was characterized by various analytic methods. Immobilized lipase was applied for biodiesel production using acidified palm oil as the starting material, the conversion rate achieved as high as 98.5% in two steps. Furthermore, the immobilized lipase displayed satisfactory stability and reusability in biodiesel production. When the aforementioned reaction was carried out in a continuous flow packed bed system, the yield of biodiesel was 94.8% and space-time yield was 2.88 g/(mL∙h). The immobilized lipase CALB@resin-CAB showed high catalytic activity and stability, which has good potential for industrial application in the field of oil processing.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"147-157"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel stepwise salinity acclimation method to improve the survival of freshwater microalgae Haematococcus lacustris in seawater salinity. 提高淡水微藻 Haematococcus lacustris 在海水盐度中存活率的新型逐步盐度适应法。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-09-26 DOI: 10.1007/s00449-024-03092-3

Qianyi Gu, Yoshiki Takayama, Noriaki Natori, Minamo Hirahara, Anupreet Kaur Chowdhary, Tatsuki Toda

{"title":"A novel stepwise salinity acclimation method to improve the survival of freshwater microalgae Haematococcus lacustris in seawater salinity.","authors":"Qianyi Gu, Yoshiki Takayama, Noriaki Natori, Minamo Hirahara, Anupreet Kaur Chowdhary, Tatsuki Toda","doi":"10.1007/s00449-024-03092-3","DOIUrl":"10.1007/s00449-024-03092-3","url":null,"abstract":"Freshwater microalga Haematococcus lacustris rich in astaxanthin, as a supplemental live diet can directly supply natural astaxanthin to the aquaculture organisms, except marine aquaculture organisms, since H. lacustris cannot tolerate seawater salinity. The objective of the present study is to provide a salinity acclimation method that allows H. lacustris to survive and accumulate astaxanthin with the aim of developing a novel supplemental live diet for marine aquaculture organisms. H. lacustris cultured in freshwater was subjected to different stepwise salinity acclimation processes (two-, three-, and four-shift). As the controls, H. lacustris was exposed to five constant salinities conditions (0, 0.05, 0.075, 0.3, and 0.6 M NaCl, respectively). Among the controls, almost all cells in the 0.3 M and 0.6 M NaCl conditions died immediately. In contrast, H. lacustris in the stepwise salinity acclimation processes survived in 0.6 M NaCl (equivalent to seawater salinity of 35 psu), showing the highest living-cell proportion (50.0%) and astaxanthin yield (0.72 mg·L-1) in the four-shift. The present study first demonstrated that H. lacustris tolerated seawater salinity through a stepwise acclimation process, proving a new strategy to supply live microalgal diets rich in natural astaxanthin for marine aquaculture.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"43-52"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristic study of Candida rugosa lipase immobilized on lignocellulosic wastes: effect of support material. 固定在木质纤维素废料上的粗毛念珠菌脂肪酶的特性研究：支撑材料的影响。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1007/s00449-024-03096-z

Viviana Chiappini, Camilla Conti, Maria Luisa Astolfi, Anna Maria Girelli

{"title":"Characteristic study of Candida rugosa lipase immobilized on lignocellulosic wastes: effect of support material.","authors":"Viviana Chiappini, Camilla Conti, Maria Luisa Astolfi, Anna Maria Girelli","doi":"10.1007/s00449-024-03096-z","DOIUrl":"10.1007/s00449-024-03096-z","url":null,"abstract":"For the first time is reported the comparison of solid biocatalysts derived from Candida rugosa lipase (CRL) immobilized on different lignocellulosic wastes (rice husk, brewer's spent grain, hemp tea waste, green tea waste, vine bark, and spent coffee grounds) focusing on the characterization of these materials and their impact on the lipase-support interaction. The wastes were subjected to meticulous characterization by ATR-FTIR, BET, and SEM analysis, besides lignin content and hydrophobicity determination. Investigating parameters influencing immobilization performance revealed the importance of morphology, textural properties, and hydrophobic interactions revealed the importance of morphology, textural properties and especially hydrophobic interactions which resulted in positive correlations between surface hydrophobicity and lipase immobilization efficiency. Hemp tea waste and spent coffee grounds demonstrated superior immobilization performances (7.20 U/g and 8.74 U/g immobilized activity, 102.3% and 33.5% efficiency, 13.4% and 15.4% recovery, respectively). Moreover, they demonstrated good temporal stability (100% and 92% residual activity after 120 days, respectively) and retained 100% of their immobilized activity after five reuses in the hydrolysis of p-nitrophenyl palmitate in hexane. In addition, the study of enzymatic desorption caused by ionic strength and detergent treatments indicated mixed hydrophobic and electrostatic interactions in rice husk, vine bark, and spent coffee grounds supports, while hemp tea waste and green tea waste were dominated by hydrophobic interactions.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"103-120"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of plastisphere on the physicochemical properties of microplastics. 塑膜对微塑料理化特性的影响。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-07-03 DOI: 10.1007/s00449-024-03059-4

Kuok Ho Daniel Tang, Ronghua Li

{"title":"The effects of plastisphere on the physicochemical properties of microplastics.","authors":"Kuok Ho Daniel Tang, Ronghua Li","doi":"10.1007/s00449-024-03059-4","DOIUrl":"10.1007/s00449-024-03059-4","url":null,"abstract":"The plastisphere is the microbial communities that grow on the surface of plastic debris, often used interchangeably with plastic biofilm or biofouled plastics. It can affect the properties of the plastic debris in multiple ways. This review aims to present the effects of the plastisphere on the physicochemical properties of microplastics systematically. It highlights that the plastisphere modifies the buoyancy and movement of microplastics by increasing their density, causing them to sink and settle out. Smaller and film microplastics are likely to settle sooner because of larger surface areas and higher rates of biofouling. Biofouled microplastics may show an oscillating movement in waterbodies when settling due to diurnal and seasonal changes in the growth of the plastisphere until they come close to the bottom of the waterbodies and are entrapped by sediments. The plastisphere enhances the adsorption of microplastics for metals and organic pollutants and shifts the adsorption mechanism from intraparticle diffusion to film diffusion. The plastisphere also increases surface roughness, reduces the pore size, and alters the overall charge of microplastics. Charge alteration is primarily attributed to changes in the functional groups on microplastic surfaces. The plastisphere introduces carbonyl, amine, amide, hydroxyl, and phosphoryl groups to microplastics, causing an increase in their surface hydrophilicity, which could alter their adsorption behaviors for heavy metals. The plastisphere may act as a reactive barrier that enhances the leaching of polar additives. It may anchor bacteria that can break down plastic additives, resulting in decreased crystallinity of microplastics. This review contributes to a better understanding of how the plastisphere alters the fate, transport, and environmental impacts of microplastics. It points to the possibility of engineering the plastisphere to improve microplastic biodegradation.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1-15"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing isoprene production by supplementing mevalonate pathway expressed in E. coli with immobilized enzymes. 通过在大肠杆菌中表达的甲羟戊酸途径中添加固定化酶来提高异戊二烯的产量。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-09-27 DOI: 10.1007/s00449-024-03093-2

Shenghu Hao, Mei Xu, Lu Li, Luyao Wang, Zhongliang Su

引用次数: 0

Inline Raman spectroscopy as process analytical technology for SARS-CoV-2 VLP production. 作为 SARS-CoV-2 VLP 生产过程分析技术的在线拉曼光谱。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-10-09 DOI: 10.1007/s00449-024-03094-1

Felipe Moura Dias, Milena Miyu Teruya, Samanta Omae Camalhonte, Vinícius Aragão Tejo Dias, Luis Giovani de Oliveira Guardalini, Jaci Leme, Thaissa Consoni Bernardino, Felipe S Sposito, Eduardo Dias, Renato Manciny Astray, Aldo Tonso, Soraia Attie Calil Jorge, Eutimio Gustavo Fernández Núñez

{"title":"Inline Raman spectroscopy as process analytical technology for SARS-CoV-2 VLP production.","authors":"Felipe Moura Dias, Milena Miyu Teruya, Samanta Omae Camalhonte, Vinícius Aragão Tejo Dias, Luis Giovani de Oliveira Guardalini, Jaci Leme, Thaissa Consoni Bernardino, Felipe S Sposito, Eduardo Dias, Renato Manciny Astray, Aldo Tonso, Soraia Attie Calil Jorge, Eutimio Gustavo Fernández Núñez","doi":"10.1007/s00449-024-03094-1","DOIUrl":"10.1007/s00449-024-03094-1","url":null,"abstract":"The present work focused on inline Raman spectroscopy monitoring of SARS-CoV-2 VLP production using two culture media by fitting chemometric models for biochemical parameters (viable cell density, cell viability, glucose, lactate, glutamine, glutamate, ammonium, and viral titer). For that purpose, linear, partial least square (PLS), and nonlinear approaches, artificial neural network (ANN), were used as correlation techniques to build the models for each variable. ANN approach resulted in better fitting for most parameters, except for viable cell density and glucose, whose PLS presented more suitable models. Both were statistically similar for ammonium. The mean absolute error of the best models, within the quantified value range for viable cell density (375,000-1,287,500 cell/mL), cell viability (29.76-100.00%), glucose (8.700-10.500 g/), lactate (0.019-0.400 g/L), glutamine (0.925-1.520 g/L), glutamate (0.552-1.610 g/L), viral titer (no virus quantified-7.505 log10 PFU/mL) and ammonium (0.0074-0.0478 g/L) were, respectively, 41,533 ± 45,273 cell/mL (PLS), 1.63 ± 1.54% (ANN), 0.058 ± 0.065 g/L (PLS), 0.007 ± 0.007 g/L (ANN), 0.007 ± 0.006 g/L (ANN), 0.006 ± 0.006 g/L (ANN), 0.211 ± 0.221 log10 PFU/mL (ANN), and 0.0026 ± 0.0026 g/L (PLS) or 0.0027 ± 0.0034 g/L (ANN). The correlation accuracy, errors, and best models obtained are in accord with studies, both online and offline approaches while using the same insect cell/baculovirus expression system or different cell host. Besides, the biochemical tracking throughout bioreactor runs using the models showed suitable profiles, even using two different culture media.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"63-84"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative investigation of the effect of mechanical and geometrical factors of a laboratory-scale bioreactor using a vibrating agitator on mammalian cell culture indices. 使用振动搅拌器的实验室规模生物反应器的机械和几何因素对哺乳动物细胞培养指数影响的定量研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-01-01 Epub Date: 2024-10-07 DOI: 10.1007/s00449-024-03095-0

Sepehr Govara, S M Hosseinalipour, Masoud Soleimani

{"title":"Quantitative investigation of the effect of mechanical and geometrical factors of a laboratory-scale bioreactor using a vibrating agitator on mammalian cell culture indices.","authors":"Sepehr Govara, S M Hosseinalipour, Masoud Soleimani","doi":"10.1007/s00449-024-03095-0","DOIUrl":"10.1007/s00449-024-03095-0","url":null,"abstract":"Mammalian cell cultures in laboratories are performed in static and dynamic methods, and cell growth indices are higher in dynamic mode. In this study, a lab-scale stirred bioreactor using a vibrating disc and a suitable setup has been introduced for dynamic cell culture, which creates proper mixing at low shear stress. 15 experiments have been done by Raji cell in batch mode using Box-Behnken design to quantitatively investigate the effect of mechanical and geometrical factors of this bioreactor on cell culture indices. Three structural factors, including disc diameter, vibration amplitude, and the height of the disc placement have been selected as the main factors. Three cell growth indices including the specific growth rate, the maximum cell concentration, and productivity have been considered as biological responses. Resulting models predict the value of each index under different settings of the factors with good accuracy. Results show that the disc diameter has the greatest effect among the investigated factors. Also, the specific growth rate, the natural logarithm of the maximum cell concentration, and productivity are about 0.033 (1/h), 13.2, and 5133 (cells/hmL), respectively by using a 25 (mm) disc with a vibration amplitude of 2.5 up to 3 (mm), and a placement height of 40 up to 60 (mm).","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"85-101"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The fed-batch production of mannosylerythritol lipids by Ustilago maydis DSM 4500 from hydrophilic carbon sources. Ustilago maydis DSM 4500 利用亲水性碳源批量生产甘露糖赤藓糖醇脂。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-09-21 DOI: 10.1007/s00449-024-03084-3

André D Valkenburg, George M Teke, Robert W M Pott, Eugéne van Rensburg

{"title":"The fed-batch production of mannosylerythritol lipids by Ustilago maydis DSM 4500 from hydrophilic carbon sources.","authors":"André D Valkenburg, George M Teke, Robert W M Pott, Eugéne van Rensburg","doi":"10.1007/s00449-024-03084-3","DOIUrl":"10.1007/s00449-024-03084-3","url":null,"abstract":"Glycolipids are a class of widely studied biosurfactants with excellent applicability in cosmetic and pharmaceutical formulations. This class of biosurfactants includes mannosylerythritol lipids (MELs), which have gained particular interest due to their moisturizing and healing activity for dry and damaged human skin, arising from conditions such as eczema. Traditionally, MELs have been produced by growing certain basidiomycetous yeasts on vegetable oils. However, oils are a comparatively expensive substrate, which negatively affects the economic performance of MEL production. In addition to this, vegetable oils significantly complicate the downstream processing required to produce a product with the required purity for most applications. To address these challenges, this study investigated MEL-A production exclusively from hydrophilic carbon sources by Ustilago maydis DSM 4500. By implementing a fed-batch production strategy, maximum MEL-A concentration of 0.87 g/L was achieved from glucose exclusively. Also, adding micronutrients (such as MnSO4) to MEL-A production showed a 24.1% increase in the product titer, implying other metabolites are formed, favoring MEL production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2043-2054"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation of Candida antarctica lipase B in metal-organic framework under ultrasound and using it to one-pot synthesis of 1,3,4,5-tetrasubstituted pyrazoles. 在超声波作用下在金属有机框架中封装白色念珠菌脂肪酶 B，并利用它一锅合成 1,3,4,5 四代吡唑。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-09-06 DOI: 10.1007/s00449-024-03083-4

Zeynab Rangraz, Ali Jafari, Mansour Shahedi, Mostafa M Amini, Zohreh Habibi

{"title":"Encapsulation of Candida antarctica lipase B in metal-organic framework under ultrasound and using it to one-pot synthesis of 1,3,4,5-tetrasubstituted pyrazoles.","authors":"Zeynab Rangraz, Ali Jafari, Mansour Shahedi, Mostafa M Amini, Zohreh Habibi","doi":"10.1007/s00449-024-03083-4","DOIUrl":"10.1007/s00449-024-03083-4","url":null,"abstract":"Encapsulating the enzyme in metal-organic frameworks (MOFs) is a convenient method to prepare MOF-enzyme biocomposite. In this study, Candida antarctica lipase B (CAL-B) was chosen to immobilize in Cu-BTC MOF under ultrasound irradiation. CAL-B was immobilized in Cu-BTC under ultrasound at 21 kHz and 11.4 W/cm2 and incubation. 98% of CAL-B was immobilized in Cu-BTC with 99 U/mg activity (threefold more active than the free CAL-B). The prepared biocomposite was characterized using FT-IR, XRD, TGA, SEM, EDX, and BET. The thermal and solvent stability of CAL-B@Cu-BTC was investigated. It was found that at a temperature of 55 ℃, CAL-B@Cu-BTC maintains its activity even after 2 h of incubation. Furthermore, in the presence of 20% and 50% concentrations of MeCN, THF, and DMF, CAL-B@Cu-BTC was found to have an activity of over 80%. A prepared biocatalyst was used to synthesize 1,3,4,5-tetrasubstituted pyrazole derivatives (50-75%) in a one-pot vessel, by adding phenyl hydrazine hydrochlorides, benzaldehydes, and dimethyl acetylenedicarboxylate.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2027-2041"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Fucoxanthin Biomanufacturing from Phaeodactylum tricornutum. 从三角藻中提取岩藻黄质生物制造技术综述。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-06-17 DOI: 10.1007/s00449-024-03039-8

Yunlong Pang, LiQin Duan, Bo Song, YuLin Cui, XiaoYong Liu, TingTing Wang

引用次数: 0