Bioprocess and Biosystems Engineering最新文献_第10页

Oleogels based on carotenoid-rich microbial oil produced by R. mucilaginosa in agro-industrial by-products. 农工副产物中富含类胡萝卜素微生物油的油凝胶研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-29 DOI: 10.1007/s00449-024-03108-y

Natália Madruga Arrieira, Mariana Teixeira de Ávila, Wladimir Hernandez Flores, Mariano Michelon, Susan Hartwig Duarte, Janaína Fernandes de Medeiros Burkert

引用次数: 0

Deep eutectic solvents assisted laccase pretreatment for improving enzymatic hydrolysis of corn stover. 深层共晶溶剂辅助漆酶预处理，改善玉米秸秆的酶水解。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-15 DOI: 10.1007/s00449-024-03102-4

Kexin Lin, Weiting Zhang, Xinyang Fan, Xiaoyan Li, Nuomeng Wang, Shuyu Yu, Lei Lu

{"title":"Deep eutectic solvents assisted laccase pretreatment for improving enzymatic hydrolysis of corn stover.","authors":"Kexin Lin, Weiting Zhang, Xinyang Fan, Xiaoyan Li, Nuomeng Wang, Shuyu Yu, Lei Lu","doi":"10.1007/s00449-024-03102-4","DOIUrl":"10.1007/s00449-024-03102-4","url":null,"abstract":"The efficient and eco-friendly removal of lignin is a critical challenge for bioethanol production from lignocellulosic biomass. Herein, we report the integration of laccase with deep eutectic solvents (DESs) for the pretreatment of corn stover to enhance the production of reducing sugars. Three betaine-based DESs were prepared and tested for their effects on the activity and stability of a bacterial laccase from Bacillus amyloliquefaciens LC02. The aqueous solution of DESs showed no adverse influence on laccase activity, and the laccase thermostability was improved in the presence of DESs. More than 95% of the laccase activity was retained in the DESs solution during the first hour of incubation at 70 °C. A red shift in the fluorescence spectra was observed for the laccase in the presence of DESs, indicating conformational changes. The laccase was able to degrade a dimeric lignin model compound by cleaving its β-O-4 bond. The transformation products were identified using LC-MS. The maximal lignin removal from corn stover was achieved by pretreatment using laccase in combination with the betaine-glycerol DES, which also resulted in a yield of fermentable sugar that was 130% higher than the control. This combination strategy provides guidance on the application of laccase and DESs in the pretreatment of lignocellulosic biomass.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"209-219"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Droplet-based bioprinting for the tailored fabrication of bacteria-laden living materials. 基于液滴的生物打印技术，用于定制制造含菌生物材料。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-22 DOI: 10.1007/s00449-024-03106-0

Xudong Guo, Dingyi Wang, Yingying Guo, Junpeng Zhang, Yingying Li, Haozhong Tian, Lihong Liu, Yong Liang, Yongguang Yin, Bin He, Ligang Hu, Guibin Jiang

{"title":"Droplet-based bioprinting for the tailored fabrication of bacteria-laden living materials.","authors":"Xudong Guo, Dingyi Wang, Yingying Guo, Junpeng Zhang, Yingying Li, Haozhong Tian, Lihong Liu, Yong Liang, Yongguang Yin, Bin He, Ligang Hu, Guibin Jiang","doi":"10.1007/s00449-024-03106-0","DOIUrl":"10.1007/s00449-024-03106-0","url":null,"abstract":"Droplet-based bioprinting (DBB) allows for high precision, noncontact, and on-demand distribution of bioinks, hence it has been widely utilized in the preparation of bacteria-laden living materials (BLMs). Nonetheless, discontinuous ink deposition makes it challenging to fabricate large-sized intact living structures via this technique. Herein, we explore the way of using DBB to construct centimeter-scale BLMs with bespoke geometries, and further demonstrate its potential applicability in sensing-responsive device by integrating engineered bacteria. We first established a DBB method based on printing-path design, which does not require hardware modification. This strategy was able to produce customized 3D-hydrogel structures with high shape fidelity. Then, we confirmed the excellent biocompatibility of the above biofabrication approach. The Escherichia coli survived 93% ± 4.0% in printed BLMs, with uniform distribution throughout the structure. As a proof-of-concept, we finally manufactured a test strip-like heavy metal biosensor capable of plug-and-play detecting mercury (II) in water using the aforesaid approach. To our knowledge, this is the first study to employ 3D bioprinted BLMs for the detection of prevalent heavy metal pollutants. Our research shed light on the versatility of DBB in BLMs construction, which is not restricted to two-dimensional patterns. Moreover, our results are expected to innovate heavy metal biodetection and improve detection efficiency and sensitivity.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"261-273"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swelling problems in immobilized filler: an improvement method and comparative study of the effect of different fillers on biotrickling filters. 固定填料的膨胀问题：改进方法和不同填料对生物滴滤器影响的比较研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-01 DOI: 10.1007/s00449-024-03101-5

Jia Liu, Qianzhu Ji, Ping Li, Shiyu Sun, Wenjun Liang

{"title":"Swelling problems in immobilized filler: an improvement method and comparative study of the effect of different fillers on biotrickling filters.","authors":"Jia Liu, Qianzhu Ji, Ping Li, Shiyu Sun, Wenjun Liang","doi":"10.1007/s00449-024-03101-5","DOIUrl":"10.1007/s00449-024-03101-5","url":null,"abstract":"Immobilized fillers have been increasingly utilized in biotrickling filters (BTFs) due to their positive impact on shock load resistance and recovery performance. However, due to the inherent characteristics of its immobilized carrier, the immobilized filler is prone to swelling during the long-term operation of the system, resulting in increased pressure drop. Polyurethane (PU) sponge was used as the cross-linked skeleton of immobilized filler and compared with direct emulsified cross-linked immobilized filler for treating ethylbenzene gas. In the early stage, both fillers can maintain good performance despite changes in the inlet concentration and short-term stagnation. However, on the 107th day of operation, the immobilized filler experienced swelling, and the pressure drop sharply increased to 137.2 Pa, while the PU immobilized filler was still able to maintain a low-pressure drop level. The results of the microbial diversity analysis revealed that the microbial community structure of PU immobilized fillers remained relatively stable when responding to the fluctuations in operating conditions. PU sponges as the skeleton can effectively prolong the service life of the immobilized filler and improve the performance of the biotrickling filter.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"193-207"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of the phenolic release and conversion in oats (Avena sativa L.) by co-microbiological fermentation with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis. 与红曲霉、酿酒酵母菌和枯草芽孢杆菌共同发酵对燕麦酚类物质释放和转化的调控

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-12-20 DOI: 10.1007/s00449-024-03109-x

Zixuan Wang, Du Luo, Wenjing Xu, Haoqun Liu, Mei Pang, Gong Chen

{"title":"Regulation of the phenolic release and conversion in oats (Avena sativa L.) by co-microbiological fermentation with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis.","authors":"Zixuan Wang, Du Luo, Wenjing Xu, Haoqun Liu, Mei Pang, Gong Chen","doi":"10.1007/s00449-024-03109-x","DOIUrl":"10.1007/s00449-024-03109-x","url":null,"abstract":"Microbial fermentation is an effective method to improve the functional activity of oats (Avena sativa L.), while there are some limitations to the advantages of single microbial fermentation. In this study, a microbial co-culture fermentation system with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis to release and conversion oat phenolics was established. Results showed that the optimal microbial co-fermentation system was obtained by adding Saccharomyces cerevisiae on the fourth day and Bacillus subtilis on the eighth day during Monascus anka fermentation (MF + 4S + 8B). The phenolic content was reached 26.93 mg GAE/g DW, which increased 41.08 times compared to un-fermented oats (UF). In the process of co-fermentation systems, cellulase and β-glucosidase (r2 = 0.97, p < 0.01) had a positive correlation with the release of phenolics. SEM combined with HPLC showed that the complex enzyme system produced by microbial co-fermentation enhanced the disruption of oat cell structure, as well as altered the phenolics fractions and facilitated the conversion of bound phenolics to free phenolics, especially the content of chlorogenic acid and vanillic acid in the free forms was increased 31.42 and 14.15 times, respectively. Additionally, the phenolic contents were increased and the components were changed with the microbial co-fermentation of crude enzyme solution further added, which validated the positive influence of complex enzyme system of MF + 4S + 8B in the phenolic release and transformation of oats. Therefore, this study systematically investigated the phenolic mobilization in oats during the co-fermentation period, which provides a viable option for improving the functional properties of cereal products, as well as the application of microbial cell factories.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"287-299"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the anaerobic sludge characteristics and inorganic impurities removal from synthesis wastewater through integration of electrocoagulation process with up-flow anaerobic sludge blanket reactor. 通过将电凝工艺与上流式厌氧污泥毯反应器相结合，提高厌氧污泥特性和合成废水中无机杂质的去除率。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-25 DOI: 10.1007/s00449-024-03104-2

Saeed Derakhshesh, Elham Abdollahzadeh Sharghi, Babak Bonakdarpour

{"title":"Enhancing the anaerobic sludge characteristics and inorganic impurities removal from synthesis wastewater through integration of electrocoagulation process with up-flow anaerobic sludge blanket reactor.","authors":"Saeed Derakhshesh, Elham Abdollahzadeh Sharghi, Babak Bonakdarpour","doi":"10.1007/s00449-024-03104-2","DOIUrl":"10.1007/s00449-024-03104-2","url":null,"abstract":"The present study investigated effects of coupling electrocoagulation (EC) process with an anaerobic digestion bioreactor, namely up-flow anaerobic sludge blanket (UASB), for the synthetic wastewater treatment. The EC-UASB mode of operation consisted of one anode and two cathodes subjected to an intermittent electrical current (10 min ON/30 min OFF) with current density of 1.5 mA/cm2. In light of this integration, the concentration of mixed liquor suspended solids and mixed liquor volatile suspended solids within anaerobic granular sludge (AGS) increased by 20.0 ± 1.4% and 12.8 ± 0.8%, respectively. The results of sludge volume index, loosely and tightly bound extracellular polymeric substances and their constituents (protein and carbohydrate) revealed that through this integration the quality of AGS has been improved. Furthermore, results of scanning electron microscopy and Fourier-transform infrared spectroscopy showed alteration in the morphology and functional groups of AGS, respectively. Additionally, this combination has demonstrated promising results in terms of performance improvement by increasing the removal efficiency of total dissolved solids by 12.1 ± 0.3% and reducing the ionic pollution in treated wastewater. However, the integration of the EC system within the UASB resulted in energy consumption and operating cost of 1.33 kWh/m3 and 0.099 USD/m3, respectively.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"233-245"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-content imaging and deep learning-driven detection of infectious bacteria in wounds. 伤口感染性细菌的高含量成像和深度学习驱动检测。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-12-02 DOI: 10.1007/s00449-024-03110-4

Ziyi Zhang, Lanmei Gao, Houbing Zheng, Yi Zhong, Gaozheng Li, Zhaoting Ye, Qi Sun, Biao Wang, Zuquan Weng

{"title":"High-content imaging and deep learning-driven detection of infectious bacteria in wounds.","authors":"Ziyi Zhang, Lanmei Gao, Houbing Zheng, Yi Zhong, Gaozheng Li, Zhaoting Ye, Qi Sun, Biao Wang, Zuquan Weng","doi":"10.1007/s00449-024-03110-4","DOIUrl":"10.1007/s00449-024-03110-4","url":null,"abstract":"Fast and accurate detection of infectious bacteria in wounds is crucial for effective clinical treatment. However, traditional methods take over 24 h to yield results, which is inadequate for urgent clinical needs. Here, we introduce a deep learning-driven framework that detects and classifies four bacteria commonly found in wounds: Acinetobacter baumannii (AB), Escherichia coli (EC), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). This framework leverages the pretrained ResNet50 deep learning architecture, trained on manually collected periodic bacterial colony-growth images from high-content imaging. In in vitro samples, our method achieves a detection rate of over 95% for early colonies cultured for 8 h, reducing detection time by more than 12 h compared to traditional Environmental Protection Agency (EPA)-approved methods. For colony classification, it identifies AB, EC, PA, and SA colonies with accuracies of 96%, 97%, 96%, and 98%, respectively. For mixed bacterial samples, it identifies colonies with 95% accuracy and classifies them with 93% precision. In mouse wound samples, the method identifies over 90% of developing bacterial colonies and classifies colony types with an average accuracy of over 94%. These results highlight the framework's potential for improving the clinical treatment of wound infections. Besides, the framework provides the detection results with key feature visualization, which enhance the prediction credibility for users. To summarize, the proposed framework enables high-throughput identification, significantly reducing detection time and providing a cost-effective tool for early bacterial detection.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"301-315"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extension of first principle elemental balancing soft-sensors by nonlinear reaction kinetics for increased robustness in bioprocess monitoring. 用非线性反应动力学扩展第一原理元素平衡软传感器以增加生物过程监测的鲁棒性。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI: 10.1007/s00449-024-03111-3

Don Fabian Müller, Daniel Wibbing, Julian Kager

{"title":"Extension of first principle elemental balancing soft-sensors by nonlinear reaction kinetics for increased robustness in bioprocess monitoring.","authors":"Don Fabian Müller, Daniel Wibbing, Julian Kager","doi":"10.1007/s00449-024-03111-3","DOIUrl":"10.1007/s00449-024-03111-3","url":null,"abstract":"A first principle soft-sensor for biomass and substrate estimation in upstream bioprocessing based on the fusion of elemental balancing and nonlinear kinetics is presented. It aims to extend the validity range of well-established elemental balancing soft sensors to substrate saturated and overfeeding conditions that often occur in induced production phases. An experimental study with recombinant E. coli cultivations was conducted to illustrate the soft-sensor principle and to analyze the accuracy as well as generalizability of the approach. Under substrate limited growth the extended soft-sensor showed similar performance as classical elemental balancing. In induced production phases however, a decline in maximum substrate uptake capacity ( <math><msub><mi>q</mi> <mrow><mi>Smax</mi></mrow> </msub> </math> ) of up to 80% was observed, where the extended soft-sensor showed up to 41 % better estimates for the biomass and up to 75 % better estimates for the substrate in terms of NRMSE. The paper discusses the possible benefits as well as the requirements for the implementation of the extended elemental balancing soft-sensor.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"317-329"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational fluid particle dynamics modeling of tangential flow filtration in perfusion cell culture. 灌注细胞培养中切向流过滤的计算流体粒子动力学建模。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2025-01-03 DOI: 10.1007/s00449-024-03112-2

Hamideh Hayati, Caitlin Kurtz, Yu Feng, Sarwat Khattak

{"title":"Computational fluid particle dynamics modeling of tangential flow filtration in perfusion cell culture.","authors":"Hamideh Hayati, Caitlin Kurtz, Yu Feng, Sarwat Khattak","doi":"10.1007/s00449-024-03112-2","DOIUrl":"10.1007/s00449-024-03112-2","url":null,"abstract":"Membrane fouling is a common and complex challenge with cell culture perfusion process in biopharmaceutical manufacturing that can have detrimental effects on the process performance. In this study, we evaluated a method to calculate the hollow fiber membrane resistance at different time points for water and supernatant. In addition, the number of subvisible particles of < 200 nm. diameter suspended in the supernatant were quantified using a nano-flow cytometry method. A computational fluid dynamics (CFD) model was developed to evaluate the impact of feed flow rate and particle count on the transmembrane pressure (TMP). Then a steady-state discrete phase model was applied to incorporate particles into the model and simulate the particles deposition over the membrane wall. The results showed an increase in the number of particles and the membrane resistance along the time course of the perfusion process. The CFD model illustrated that more particle deposition was observed at lower feed stream flow rates. The fraction of deposited particle was reduced by > 50% when the feed flow rate was increased from 35 ml/min to 300 ml/min. Our findings suggest that the total number of subvisible particles has a significant impact on TMP and membrane resistance and, thus, could play a major role in the mechanism of membrane fouling. CFD modeling can be a useful tool to predict the behavior of a process in a specific membrane. CFD simulations could also be used to optimize process parameters to improve membrane cleanability, reduce particle deposition, and reduce the risk of membrane fouling.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"331-342"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of palm sludge oil for rhamnolipid biosynthesis by Pseudomonas aeruginosa USM-AR2 in a stirred tank reactor. 铜绿假单胞菌 USM-AR2 在搅拌罐反应器中利用棕榈污泥油进行鼠李糖脂生物合成。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-13 DOI: 10.1007/s00449-024-03103-3

Mohd Shafiq Nasir, Ahmad Ramli Mohd Yahya, Nur Asshifa Md Noh

{"title":"Utilization of palm sludge oil for rhamnolipid biosynthesis by Pseudomonas aeruginosa USM-AR2 in a stirred tank reactor.","authors":"Mohd Shafiq Nasir, Ahmad Ramli Mohd Yahya, Nur Asshifa Md Noh","doi":"10.1007/s00449-024-03103-3","DOIUrl":"10.1007/s00449-024-03103-3","url":null,"abstract":"The study focused on rhamnolipid production by batch fermentation of Pseudomonas aeruginosa USM-AR2 in a 3-L stirred-tank reactor (STR) using palm sludge oil (PSO) as the sole carbon source. The impact of various agitation rates towards the dispersion of PSO in the medium was evaluated to improve biomass growth and rhamnolipid production. A mechanical foam collection and recycling system was designed and retrofitted to the STR to overcome severe foam formation during fermentation. The maximum biomass produced was 11.29 ± 0.20 g/L obtained at 400 rpm, while the maximum rhamnolipid production was 5.06 ± 1.17 g/L at 600 rpm, giving a rhamnolipid productivity of 0.023 g/L/h. High agitation enhances substrate availability by breaking the hydrophobic semi-solid PSO into smaller substrate particles, increasing surface contact area, thus facilitating the PSO utilisation by P. aeruginosa USM-AR2, thereby inducing rhamnolipid production. This study further demonstrates the ability of rhamnolipid to solubilize and disperse sludge oil, which typically remains a solid at room temperature, in the liquid medium. GCMS analysis showed that five fatty acids, namely palmitic acid, myristic acid, stearic acid, methyl ester and linoleic acid, have been utilised. The rhamnolipid showed an oil spreading test result of 160 mm of waste engine oil displacement compared to control using distilled water that remained non-displaced, and a critical micelle concentration (CMC) of 17 mg/L. In emulsification index (E24) assay, the rhamnolipid was shown to emulsify toluene (66.7% ± 7.2), waste engine oil (58.3% ± 7.2), kerosene (41.8% ± 4.8) and n-hexane (33.1% ± 5.7). UPLC analysis on rhamnolipid revealed a congener mixture of rhamnolipid, namely di-rhamnolipid and mono-rhamnolipid mixture. This is the first report on the employment of an integrated foam control reactor system with PSO as the carbon source for rhamnolipid production by P. aeruginosa USM-AR2 culture.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"221-232"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0