Bioprocess and Biosystems Engineering最新文献

{"title":"The effects of plastisphere on the physicochemical properties of microplastics.","authors":"Kuok Ho Daniel Tang, Ronghua Li","doi":"10.1007/s00449-024-03059-4","DOIUrl":"https://doi.org/10.1007/s00449-024-03059-4","url":null,"abstract":"<p><p>The plastisphere is the microbial communities that grow on the surface of plastic debris, often used interchangeably with plastic biofilm or biofouled plastics. It can affect the properties of the plastic debris in multiple ways. This review aims to present the effects of the plastisphere on the physicochemical properties of microplastics systematically. It highlights that the plastisphere modifies the buoyancy and movement of microplastics by increasing their density, causing them to sink and settle out. Smaller and film microplastics are likely to settle sooner because of larger surface areas and higher rates of biofouling. Biofouled microplastics may show an oscillating movement in waterbodies when settling due to diurnal and seasonal changes in the growth of the plastisphere until they come close to the bottom of the waterbodies and are entrapped by sediments. The plastisphere enhances the adsorption of microplastics for metals and organic pollutants and shifts the adsorption mechanism from intraparticle diffusion to film diffusion. The plastisphere also increases surface roughness, reduces the pore size, and alters the overall charge of microplastics. Charge alteration is primarily attributed to changes in the functional groups on microplastic surfaces. The plastisphere introduces carbonyl, amine, amide, hydroxyl, and phosphoryl groups to microplastics, causing an increase in their surface hydrophilicity, which could alter their adsorption behaviors for heavy metals. The plastisphere may act as a reactive barrier that enhances the leaching of polar additives. It may anchor bacteria that can break down plastic additives, resulting in decreased crystallinity of microplastics. This review contributes to a better understanding of how the plastisphere alters the fate, transport, and environmental impacts of microplastics. It points to the possibility of engineering the plastisphere to improve microplastic biodegradation.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum OL5 biosurfactants as alternative to chemical surfactants for application in eco-friendly cosmetics and skincare products.","authors":"Ameni Ben Ammar, Mariem Ben Ali, Boutheina Cherif, Bochra Gargouri, Yosra Tahri, Souad Rouis, Dhouha Ghribi","doi":"10.1007/s00449-024-03022-3","DOIUrl":"10.1007/s00449-024-03022-3","url":null,"abstract":"<p><p>Cosmetics have been extremely popular throughout history and continue to be so today. Cosmetic and personal care products, including toothpaste, shampoo, lotions, and makeup, are typically made with petroleum-based surfactants. Currently, there is an increasing demand to enhance the sustainability of surface-active compounds in dermal formulations. Biosurfactants, derived from living cells, are considered more environmentally friendly than synthetic surfactants. Thus, the use of biosurfactants is a promising strategy for formulating more environmentally friendly and sustainable dermal products. Biosurfactants have the potential to replace chemical surface-active agents in the cosmetic sector due to their multifunctional qualities, such as foaming, emulsifying, and skin-moisturizing activities.In this study, two glycolipopeptide biosurfactants derived from Lactiplantibacillus plantarum OL5 were used as stabilizing factors in oil-in-water emulsions in the presence of coconut oils. Both biosurfactants increased emulsion stability, particularly in the 1:3 ratio, dispersion, and droplet size. Moreover, the cytotoxicity of the two Lactiplantibacillus plantarum biosurfactants was assessed on B lymphocytes and MCF-7 cells. Overall, the results gathered herein are very promising for the development of new green cosmetic formulations.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1039-1056"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic acid production by Streptococcus zooepidemicus MW26985 using potato peel waste hydrolyzate.","authors":"Seyedali Mousavi, Razieh Esfandiar, Ghasem Najafpour-Darzi","doi":"10.1007/s00449-024-03007-2","DOIUrl":"10.1007/s00449-024-03007-2","url":null,"abstract":"<p><p>In this research, we examined the production of hyaluronic acid (HA) by Streptococcus zooepidemicus strain MW26985 using different substrates and potato peel waste (PPW) as an affordable substrate. First, culture medium components, including carbon and nitrogen sources, were optimized for bacterial HA production. Five different carbon sources (glucose, sucrose, lactose, sago starch, and potato starch, at a concentration of 30 g/L) and three distinct nitrogen sources (peptone, yeast extract, and ammonium sulfate, at a concentration of 10 g/L) were investigated. Glucose, among the carbon sources, and yeast extract, among nitrogen sources, produced the most HA which was determined as 1.41 g/L. Afterward, potato peel sugars were extracted by dilute acid and enzymatic hydrolysis and then employed as a cost-effective carbon source for the growth of S. zooepidemicus. Based on the results, the fermentation process yielded 0.59 g/L HA from potato peel sugars through acid hydrolysis and 0.92 g/L HA from those released by enzymatic hydrolysis. The supplementation of both hydrolyzates with glucose as an additional carbon source enhanced HA production to 0.95 g/L and 1.18 g/L using acidic and enzymatic hydrolyzates, respectively. The cetyltrimethylammonium bromide (CTAB) turbidimetric method was used to evaluate the concentration of HA in the fermentation broth using the colorimetric method. Also, the peaks observed by Fourier transform infrared (FTIR) spectroscopy confirmed that the exopolysaccharide (EPS) was composed of HA. These observations demonstrate that potato peel residues can be a novel alternative as a carbon source for the economical production of HA by S. zooepidemicus.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1003-1015"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-based biofuel production from low-cost lignocellulose biomass: environmental sustainability and economic approach.","authors":"Selvakumar Sakthivel, Kanthimathi Muthusamy, Amutha Priya Thangarajan, Muthu Thiruvengadam, Baskar Venkidasamy","doi":"10.1007/s00449-024-03005-4","DOIUrl":"10.1007/s00449-024-03005-4","url":null,"abstract":"<p><p>The use of nanomaterials in biofuel production from lignocellulosic biomass offers a promising approach to simultaneously address environmental sustainability and economic viability. This review provides an overview of the environmental and economic implications of integrating nanotechnology into biofuel production from low-cost lignocellulosic biomass. In this review, we highlight the potential benefits and challenges of nano-based biofuel production. Nanomaterials provide opportunities to improve feedstock pretreatment, enzymatic hydrolysis, fermentation, and catalysis, resulting in enhanced process efficiency, lower energy consumption, and reduced environmental impact. Conducting life cycle assessments is crucial for evaluating the overall environmental footprint of biofuel production. An economic perspective that focuses on the cost implications of utilizing nanomaterials in biofuel production is also discussed. A comprehensive understanding of both environmental and economic dimensions is essential to fully harness the potential of nanomaterials in biofuel production from lignocellulosic biomass and to move towards sustainable future energy.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"971-990"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimized for production of iturin A in biofilm reactor by Bacillus velezensis ND.","authors":"Zhongmin Tang, Leiming Wang, Zhengjun Xiong, Yuxia Zhu, Huili Zhang","doi":"10.1007/s00449-024-03038-9","DOIUrl":"10.1007/s00449-024-03038-9","url":null,"abstract":"<p><p>In this research, to provide an optimal growth medium for the production of iturin A, the concentrations of key amino acid precursors were optimized in shake flask cultures using the response surface method. The optimized medium were applied in a biofilm reactor for batch fermentation, resulting in enhanced production of iturin A. On this basis, a step-wise pH control strategy and a combined step-wise pH and temperature control strategy were introduced to further improve the production of iturin A. Finally, the fed-batch fermentation was performed based on combined step-wise pH and temperature control. The titer and productivity of iturin A reached 7.86 ± 0.23 g/L and 65.50 ± 1.92 mg/L/h, respectively, which were 37.65 and 65.20% higher than that before process optimization.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1095-1105"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Batch and semi-continuous treatment of cassava wastewater using microbial fuel cells and metataxonomic analysis.","authors":"Juan Carlos Quintero-Díaz, Jorge Omar Gil-Posada","doi":"10.1007/s00449-024-03025-0","DOIUrl":"10.1007/s00449-024-03025-0","url":null,"abstract":"<p><p>The treatment of agroindustrial wastewater using microbial fuel cells (MFCs) is a technological strategy to harness its chemical energy while simultaneously purifying the water. This manuscript investigates the organic load effect as chemical oxygen demand (COD) on the production of electricity during the treatment of cassava wastewater by means of a dual-chamber microbial fuel cell in batch mode. Additionally, specific conditions were selected to evaluate the semi-continuous operational mode. The dynamics of microbial communities on the graphite anode were also investigated. The maximum power density delivered by the batch MFC (656.4 μW m <math><msup><mrow></mrow> <mrow><mo>-</mo> <mn>2</mn></mrow> </msup> </math> ) was achieved at the highest evaluated organic load (6.8 g COD L <math><msup><mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> ). Similarly, the largest COD removal efficiency (61.9%) was reached at the lowest organic load (1.17 g COD L <math><msup><mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> ). Cyanide degradation percentages (50-70%) were achieved across treatments. The semi-continuous operation of the MFC for 2 months revealed that the voltage across the cell is dependent on the supply or suspension of the organic load feed. The electrode polarization resistance was observed to decreases over time, possibly due to the enrichment of the anode with electrogenic microbial communities. A metataxonomic analysis revealed a significant increase in bacteria from the phylum Firmicutes, primarily of the genus Enterococcus.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1057-1070"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of loading rate and pH on glycerol fermentation and microbial population in an upflow anaerobic filter reactor.","authors":"Cândida N Cordeiro, Patricia Rojas, Shyrlane T S Veras, Mario T Kato, Lourdinha Florencio, José Luis Sanz","doi":"10.1007/s00449-024-03003-6","DOIUrl":"10.1007/s00449-024-03003-6","url":null,"abstract":"<p><p>A reactor with silicone tubes as support medium was used for glycerol fermentation. The experimental set-up consisted of three phases. In P1, the applied glycerol loading rate (gly-LR) was in the range of 6-10 g.L^-1.d^-1 at an influent pH of 7.9 ± 0.4. In P2, gly-LR was kept constant (18.0 ± 1.8 g.L^-1.d^-1) with different doses of NaHCO₃. Finally in P3, two different gly-LR (9 and 18 g.L^-1.d^-1) were evaluated, dosing 1 g-NaHCO₃ per g-COD of glycerol. Glycerol consumption was close 90%. The main end-product was 1,3-propanediol (1,3-PDO) (0.40 mol.mol-gly^-1), but ethanol was also generated, particularly at pH above 8 and low gly-LR (0.20 mol.mol-gly^-1). After 1-year operation with glycerol as the only carbon source, a drastic shift in the bacterial community was observed. The 1,3-PDO producers Lacrimispora and Clostridium became dominant, although non-glycerol-degrading fermentative genera, e.g., Actinomyces and Eubacterium, thrived at the expense of cellular breakdown products.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"991-1002"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-state fermentation using wheat bran to produce glucose syrup and functional cereal bars.","authors":"Célia Cristina Malaguti Figueiredo, Filipe Oliveira Granero, Luciana Pereira Silva, Ilca Fabiane Amâncio Nogueira, Joyce Faria de Souza, Bruna Escaramboni, Pedro de Oliva Neto, Regildo Márcio Gonçalves da Silva","doi":"10.1007/s00449-024-03032-1","DOIUrl":"10.1007/s00449-024-03032-1","url":null,"abstract":"<p><p>Wheat bran is one of the most abundant by-products from grain milling, which can be used as substrate for solid-state fermentation (SSF) to obtain enzymes able to convert this agro-industrial waste into glucose syrup, which in turn can be applied for the production of different food products. The present study aimed to determine centesimal composition of wheat bran, obtain enzymatic extract that converts wheat bran into wheat glucose syrup (WGS), produce rice flakes cereal bars (RFCB), and evaluate their nutritional composition and the presence of functional compounds, as well as their antioxidant potential. Determination of centesimal composition of wheat bran demonstrated its nutritional potential. Enzymatic extract was obtained and it converted wheat bran into WGS, which were applied to rice flakes producing RFCB. These cereal bars proved to be a source of dietary fiber (1.8 g) and soluble protein (7.2 g) while RCFB produced with corn glucose syrup did not present these nutritional components. In addition, RFCB produced with WGS showed polyphenolic compounds, among them flavonoids, which exhibited antioxidant activity by DPPH and ABTS radical scavenging (47.46% and 711.89 μM Trolox Equivalent/g, respectively), and iron ion reduction (71.70 μM Trolox equivalent/g). Final product showed a decrease in caloric value and sodium content. Therefore, the present study showed that the bioprocess of SSF yields a nutritional, ecological, and functional food product, which might be of great interest for food industry, adding nutritional and functional value to a well-stablished product.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1081-1094"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable menaquinone-7 production through continuous fermentation in biofilm bioreactors.","authors":"Aydin Berenjian, Ehsan Mahdinia, Ali Demirci","doi":"10.1007/s00449-024-03040-1","DOIUrl":"10.1007/s00449-024-03040-1","url":null,"abstract":"<p><p>Menaquinone-7 (MK-7), a vital vitamin with numerous health benefits, is synthesized and secreted extracellularly by the formation of biofilm, dominantly in Bacillus strains. Our team developed an innovative biofilm reactor utilizing Bacillus subtilis natto cells to foster biofilm growth on plastic composite supports to produce MK-7. Continuous fermentation in biofilm reactors offers a promising strategy for achieving sustainable and efficient production of Menaquinone-7 (MK-7). Unlike conventional batch fermentation, continuous biofilm reactors maintain a steady state of operation, which reduces resource consumption and waste generation, contributing to sustainability. By optimizing fermentation conditions, MK-7 production was significantly enhanced in this study, demonstrating the potential for sustainable industrial-scale production. To determine the optimal operational parameters, various dilution rates were tested. These rates were selected based on their potential to enhance nutrient supply and biofilm stability, thereby improving MK-7 production. By carefully considering the fermentation conditions and systematically varying the dilution rates, MK-7 production was significantly enhanced during continuous fermentation. The MK-7 productivity was found to increase from 0.12 mg/L/h to 0.33 mg/L/h with a dilution rate increment from 0.007 to 0.042 h^-1). This range was chosen to explore the impact of various nutrient supply rates on MK-7 production and to identify the optimal conditions for maximizing productivity. However, a further increase in the dilution rate to 0.084 h^-1 led to reduced productivity at approximately 0.16 mg/L/h, likely due to insufficient retention time for effective biofilm formation. Consequently, a dilution rate of 0.042 h^-1 exhibited the highest productivity of 0.33 mg/L/h, outperforming all investigated dilution rates and demonstrating the critical balance between nutrient supply and retention time in continuous fermentation. These findings validate the feasibility of operating continuous fermentation at a 0.084 h^-1 dilution rate, corresponding to a 48 h retention time, to achieve the highest MK-7 productivity compared to conventional batch fermentation. The significant advancements achieved in enhancing Menaquinone-7 (MK-7) productivity through continuous fermentation at optimal dilution rates in the present work indicate promising prospects for even greater efficiency and sustainability in MK-7 production through future developments.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1107-1116"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community structure of an anaerobic side-stream coupled anoxic-aerobic membrane bioreactor (AOMBR-ASSR) for an in-situ sludge reduction process.","authors":"Xinqiang Ning, Jialun Hu, Jiao Yue, Tang Tang, Bin Zhang","doi":"10.1007/s00449-024-03019-y","DOIUrl":"10.1007/s00449-024-03019-y","url":null,"abstract":"<p><p>With the anoxic-aerobic membrane bioreactor (AO-MBR, CP) as a reference, high-throughput sequencing technology was used to reveal the characteristics of the microbial community structure in the anaerobic side-stream anoxic-aerobic membrane bioreactor sludge reduction process (AOMBR-ASSR, SRP). After the stable operation of two processes for 120 days, the average removal efficiencies of TN and TP in the effluent of SRP were increased by 5.6% and 29.8%, respectively. The observed sludge yields (Y_obs) of the two processes were 0.14 and 0.17 gMLSS/(gCOD), respectively, and the sludge reduction rate of the SRP was 19.5%. Compared to the CP, the microbial richness and diversity index of SRP increased significantly. Chloroflexi, which is responsible for the degradation of organic substances under an anaerobic condition, seemed to be reduced in the SRP. Meanwhile, other phyla that involved in the nitrogen cycle, such as Nitrospirae and Planctomycetes, were found to be more abundant in the SRP than in the CP. A total of 21 identified classes were observed, and primarily hydrolyzed fermented bacteria (Sphingobacteriia, Betaproteobacteria, Actinobacteria and Deltaproteobacteria) and slow-growing microorganisms (Bacilli) were accumulated in the SRP. At the genus level, the inserted anaerobic side-stream reactor favored the hydrolyzed bacteria (Saprospiraceae, Rhodobacter and Candidatus_Competibacter), fermented bacteria (Lactococcus and Trichococcus), and slow-growing microorganisms (Dechloromonas and Haliangium), which play a crucial role in the sludge reduction. Furthermore, the enrichment of bacterial species related to nitrogen (Nitrospir and Azospira) provided the potential for nitrogen removal, while the anaerobic environment of the side-stream reactor promoted the enrichment of phosphorus-accumulating organisms.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1027-1037"},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}