Bioprocess and Biosystems Engineering最新文献

{"title":"Asphaltenes biodegradation from heavy crude oils by the yeast Yarrowia lipolytica.","authors":"Filipe Smith Buarque, Júlio Cesar Soares Sales, Lívia Cabral Lobo, Erika Christina Ashton Nunes Chrisman, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho","doi":"10.1007/s00449-024-03114-0","DOIUrl":"10.1007/s00449-024-03114-0","url":null,"abstract":"<p><p>Heavy crude oil reserves are characterized by their high viscosity and density, largely due to significant quantities of asphaltenes. The removal of asphaltene precipitates from oil industry installations is crucial, as they can contaminate catalysts and obstruct pipelines. Therefore, this study aimed to bio-transform heavy oil asphaltenes into smaller molecules using the yeast Yarrowia lipolytica, known for its ability to efficiently degrade hydrophobic substrates. For this purpose, asphaltenes were extracted from crude oil samples, and yeast growth was assessed in a mineral medium containing 2, 5, or 10 g L^-1 of asphaltenes. After 168 h of incubation, liquid-liquid extraction was conducted on samples from the Yarrowia lipolytica growth medium using chloroform. The extracted fractions were then quantified by gas chromatography. The results indicated that the yeast could utilize the asphaltenes as a carbon source for growth, though there was a delay in growth compared to the control (glucose as the carbon source), with a maximum biomass concentration of 2.26 g L^-1 achieved at 144 h. From the experimental design, it was determined that a higher concentration of aromatic compounds was achieved under the conditions of 115 rpm, 2 g L^-1 of asphaltenes, and 0.5 g L^-1 of cell inoculum. Conversely, to obtain a higher concentration of saturated compounds, the optimal conditions were 160 rpm, 5 g L^-1 of asphaltenes, and 1.0 g L^-1 of cell inoculum. Molecular docking results indicated that asphaltenes have a high affinity for cytochrome P450, laccase, and Lip2, with interactions observed with their catalytic triads, suggesting a significant role for these enzymes in asphaltene bioconversion.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"381-394"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced physicochemical characteristics and biological activities of low-temperature ethylenediamine/urea pretreated lignin.","authors":"Yongkang Pan, Weiwei Chen, Qiaozhen Kang, Limin Hao, Jike Lu, Jiaqing Zhu","doi":"10.1007/s00449-024-03113-1","DOIUrl":"10.1007/s00449-024-03113-1","url":null,"abstract":"<p><p>Low-temperature ethylenediamine (EDA)/urea pretreatment had been demonstrated to be an efficient pretreatment method for enzymatic hydrolysis and bioethanol production. For high-value utilization of the third main components of lignocellulosic biomass, the physicochemical structure characteristics and biological activities of low-temperature EDA/urea pretreated lignin (EUL) were comprehensively investigated in the present study. The results demonstrated that the pretreatment process facilitated the depolymerization of lignin, resulting in notable reduction in molecular weight and polydispersity index from 2.32 to 1.42 kg/mol and 1.44 to 1.20, respectively. The EDA/urea pretreated lignin (EUL) exhibited enhanced ultraviolet absorption capacity and the most significant DPPH radical scavenging and inhibition of Staphylococcus aureus in comparison to the primary lignin (PL) and the NaOH pretreated lignin (NL). Enhanced physicochemical characteristics and biological activities of EUL make it more suitable to be developed as sunscreen ingredient or antioxidant and antimicrobial agent in food preservation and conservation.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"367-379"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.","authors":"Luca Antonia Grebe, Paul Richter, Torben Altenkirch, Marcel Mann, Markus Jan Müller, Jochen Büchs, Jørgen Barsett Magnus","doi":"10.1007/s00449-024-03117-x","DOIUrl":"10.1007/s00449-024-03117-x","url":null,"abstract":"<p><p>The transition towards sustainable bioprocesses requires renewable feedstocks to reduce dependency on finite resources. While plant-based feedstocks offer significant potential, their complex composition poses new challenges. The microorganisms often exhibit polyauxic growth when presented with multiple carbon sources simultaneously, consuming them in a distinct order according to their carbon source preferences. The traditional investigation of polyauxic growth involves laborious sampling and offline analysis, hindering high-throughput screenings. This study introduces an efficient method for identifying carbon source consumption and their order of metabolization by various microorganisms using the respiration activity monitoring system (RAMOS) in shake flasks. As aerobic carbon metabolization and oxygen consumption are strictly correlated, the characteristic phases of polyauxic growth are visible in the oxygen transfer rate (OTR) and can be assigned to the respective carbon sources. An extended 16-flask RAMOS enables real-time monitoring of microbial respiration on up to seven carbon sources and one reference cultivation simultaneously, thus providing crucial insights into their metabolization without extensive sampling and offline analysis. The method's accuracy was validated against traditional high-performance liquid chromatography (HPLC). Its applicability to both fast-growing Escherichia coli (investigated carbon sources: glucose, arabinose, sorbitol, xylose, and glycerol) and slow-growing Ustilago trichophora (glucose, glycerol, xylose, sorbitol, rhamnose, galacturonic acid, and lactic acid) was demonstrated. Additionally, it was successfully applied to the plant-based second-generation feedstock corn leaf hydrolysate, revealing the bioavailability of the included carbon sources (glucose, sucrose, arabinose, xylose, and galactose) and their order of metabolization by Ustilago maydis.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"413-425"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the power of microbial fuel cells as pioneering green technology: advancing sustainable energy and wastewater treatment through innovative nanotechnology.","authors":"Hadeer E Ali, Bahaa A Hemdan, Mehrez E El-Naggar, Mohamed Azab El-Liethy, Dipak A Jadhav, Hoda H El-Hendawy, M Ali, Gamila E El-Taweel","doi":"10.1007/s00449-024-03115-z","DOIUrl":"10.1007/s00449-024-03115-z","url":null,"abstract":"<p><p>The purpose of this review is to gain attention about intro the advanced and green technology that has dual action for both clean wastewater and produce energy. Water scarcity and the continuous energy crisis have arisen as major worldwide concerns, requiring the creation of ecologically friendly and sustainable energy alternatives. The rapid exhaustion of fossil resources needs the development of alternative energy sources that reduce carbon emissions while maintaining ecological balance. Microbial fuel cells (MFCs) provide a viable option by producing power from the oxidation of organic and biodegradable chemicals using microorganisms as natural catalysts. This technology has sparked widespread attention due to its combined potential to cleanse wastewater and recover energy. The review presents a complete examination of current advances in MFCs technology, with a focus on the crucial role of anode materials in improving their performance. Moreover, different anode materials and their nanoscale modifications are being studied to boost MFC efficiency. This current review also focused on the effects of surface modifications and different anode compositions on power generation and system stability. It also investigates the electrochemical principles behind these enhancements, providing insights into the economic potential of MFCs. MFCs provide a long-term solution to energy and environmental issues by addressing both wastewater treatment and energy production.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"343-366"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upflow blanket filter anammox (UBFA) system treating low-nitrogen wastewater: high-efficient nitrogen removal, granules formation, N₂O emission, and microbial succession.","authors":"Chongyang Wang, Feng Gao, Sheng Gao, Zheng Nian, Xintong Han","doi":"10.1007/s00449-024-03116-y","DOIUrl":"10.1007/s00449-024-03116-y","url":null,"abstract":"<p><p>This research provides an important approach for low-nitrogen wastewater treatment through anaerobic ammonium oxidation (Anammox), and Anammox granule sludge (AnGS) in the Upflow. Blanket Filter Anammox (UBFA) system through shortening the hydraulic retention time was successfully cultivated. The percentage of medium granules (1.0-2.0 mm) with the highest Anammox activity increased from 0 to 28.5%, and the proportion of flocs (0-200 μm) reduced from 84.5% to 17.6%. Through the multidimensional analysis of AnGS, the relationship between AnGS and EPS secretion, low SVI, high PN/PS, multiple filamentous bacteria, and AnAOB were explored. Microelectrode tracing tests demonstrated that the main anammox reaction active layer was 0-1500 μm, and the highest activity was observed at 200-400 μm, whereas denitrification activity and N₂O production were mainly distributed in the granules deep layer of 1500-2500 μm. The research showed that Candidatus Brocadia and Candidatus Kuenenia were the predominant anammox species in the UBFA system, while the abundance of AnAOB was higher in medium granules.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"395-412"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gamma-aminobutyric acid fermentation and its fermented extracts on α-glucosidase inhibition and anti-obesity effect.","authors":"Ji Min Kim, Chae Hun Ra","doi":"10.1007/s00449-024-03119-9","DOIUrl":"10.1007/s00449-024-03119-9","url":null,"abstract":"<p><p>Levilactobacillus brevis KCL010 was fermented in a simple medium containing 8% (w/v) of rice bran extract. We modified the carbon, nitrogen, and initial pH conditions using 10 g/L of sucrose, 10 g/L of yeast extract, and 5.0 of pH, respectively. To minimize the pH increase due to decarboxylation, we fermented 100 mL of modified synthetic medium containing citrate-phosphate buffer (CPB, pH 5.0) of 25-200 mM in 250 mL Erlenmeyer flasks. After 72 h of fermentation with 50 mM CPB, the maximum GABA concentration and conversion efficiency were 3.42 g/L and 22.39%. Furthermore, the potential α-glucosidase inhibitory activity, MTT assay, and oil red O staining were determined by fermented extracts of L. brevis KCL010. At the highest concentration of 500 μg/mL, the α-glucosidase inhibition percentages for non-fermented rice bran (NFRB), rice bran fermented by L. brevis (RBFL), and GABA (analytical standard) extracts were 55.03%, 58.37%, and 59.48%, respectively. All extracts exceeded 80% viability, suggesting that there was no cytotoxic to 3T3-L1 adipocytes. The rice bran fermented by L. brevis (RBFL) extract shows a high inhibition of lipid accumulation by 29.33% compared to those of extracts.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"437-444"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess development for microbial production and purification of cellobiose lipids by the smut fungus Ustilago maydis DSM 4500.","authors":"André D Valkenburg, George M Teke, Eugéne van Rensburg, Robert W M Pott","doi":"10.1007/s00449-025-03127-3","DOIUrl":"10.1007/s00449-025-03127-3","url":null,"abstract":"<p><p>Cellobiose lipids (CBLs) are a class of glycolipid biosurfactants produced by various fungal strains. These compounds have gained significant interest due to their surface-active and antifungal properties, which are comparable to traditional synthetic surfactants and antimicrobials. Despite their potential applicability in various cosmetic, pharmaceutical, and agricultural formulations, significantly less research has been focused on their production and purification in comparison to other glycolipid biosurfactants, such as mannosylerythritol lipids (MELs) and sophorolipids. Hence, this work proposes the development of a bioprocess that involves the microbial production and high-level chromatographic purification of CBLs from a submerged culture of Ustilago maydis DSM 4500. After a highly purified CBL product was obtained, the factors affecting the production of this glycolipid were investigated. It was demonstrated that U. maydis DSM 4500 produces a specific structural variant of CBLs at a concentration of 1.36 g/L on an optimized the growth medium. Also, it was established that when the C/N ratio was decreased, the CBL titer increased by 2.3-fold. Furthermore, supplementing the culture with ZnSO₄ at a concentration of 0.04 mg/L further increased CBL concentration to 4.95 g/L, representing the highest CBL titer achieved in a single-stage bioprocess to date. This study developed a methodology for utilizing U. maydis as a high-level CBL producer, which could challenge other familiar CBL producers, such as Sporisorium scitamineum and Cryptococcus humicola.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"509-520"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a multi-scale nanofiber scaffold platform for structurally and functionally replicated artificial perforating arteries.","authors":"Su Jin Yoon, Jae Ahn Shin, Hwa Sung Shin","doi":"10.1007/s00449-024-03122-0","DOIUrl":"10.1007/s00449-024-03122-0","url":null,"abstract":"<p><p>Experimental models for exploring abnormal brain blood vessels, including ischemic stroke, are crucial in neuroscience; recently, significant attention has been paid to artificial tissues through tissue engineering. Nanofibers, although commonly used as tissue engineering scaffolds, undergo structural deformations easily, making it challenging to create uniform tissue, especially for the smallest-diameter ones such as perforating arteries. This study focused on the development of a platform capable of reconstructing structurally and functionally replicated perforating arteries. To ensure structural consistency, 3D-printed modules were developed to minimize the structural deformation of nanofibrous scaffolds when integrated into a 3D-printed vessel culture dish. Surface structures and physical characteristics of the nanofibers before and after installation were compared using scanning electron microscopy, contact angle analysis, surface area analysis, and universal testing machine (UTM) analysis. The results showed a uniform thickness distribution, topography, maximum load, tensile strain, tensile strength, surface area, pore size, and pore volume of the nanofibers. For consistency in tissue culture, smooth muscle, endothelial, and astrocyte cells were co-cultured by continuously measuring the pH of the medium and replenishing the depleted glucose using the Kalman filter control system. The functional efficacy and consistency of the artificial perforating vessels were confirmed under oxidative stress induced by exposure to hydrogen peroxide. Transcriptional mRNA expression trends were similar to those in vivo for antioxidant enzymes, neurotrophic factors, inflammatory factors, and endothelial cell activation factors, with very low variation between tissues. This study provides a research platform for studying the oxidative stress environments related to stroke by mass-producing perforating arteries with consistent structures and functions.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"483-492"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of FK520 production in Streptomyces hygroscopicus var. ascomyceticus ATCC 14891 by overexpressing the regulatory gene fkbR2.","authors":"Xue Xiao, Yu Fu, Daojing Zhang, Shuhong Gao","doi":"10.1007/s00449-024-03124-y","DOIUrl":"10.1007/s00449-024-03124-y","url":null,"abstract":"<p><p>Ascomycin (FK520) is a 23-membered macrolide antibiotic primarily produced by the Streptomyces hygroscopicus var. ascomyceticus. Structurally similar to tacrolimus and rapamycin, it serves as an effective immunosuppressant widely used in the treatment of rejection reactions after organ transplantation and certain autoimmune diseases. Currently, FK520 is mainly produced through microbial fermentation, but its yield remains low. Since the gene fkbR2 is a regulatory gene within the FK520 biosynthesis gene cluster that has not been studied, this paper focuses on the overexpression of the gene fkbR2 in Streptomyces hygroscopicus var. ascomyceticus ATCC 14891 (WT). By constructing a strain with overexpressed fkbR2 gene, we initially obtained a high-yield strain R2-17 through shake flask fermentation, with a 28% increase in yield compared to WT. In the process of further improving the stability of the high-yield strain, this paper defines two indices: high-yield index and stability index. After two consecutive rounds of natural breeding, strain R2-17 achieved a high-yield index of 100% and a stability index of 80%. Finally, the high-yield strain R2-17-3-10 was successfully screened, and the yield was increased by 34% compared with the strain WT, reaching 686.47 mg/L. A comparative analysis between the high-yield strain R2-17-3-10 and the original strain WT revealed differences in fermentation process parameters such as FK520 synthesis rate, pH, bacterial growth, glycerol consumption rate, ammonia nitrogen level, and ammonium ion concentration. In addition, the transcription levels of genes involved in the synthesis of precursors 4,5-dihydroxycyclohex-1-enecarboxylic acid (fkbO), ethylmalonyl-CoA (fkbE, fkbU, fkbS), and pipecolic acid (fkbL), as well as pathway-specific regulatory genes (fkbN, fkbR1), were significantly increased at different time points in the high-yield strain R2-17-3-10. EMSAs analysis showed that the FkbR2 protein could not bind to the promoter region of above genes. This suggests that the gene fkbR2 may enhance the supply of FK520 synthetic precursors by indirectly regulating the transcription levels of these genes, thereby promoting an increase in FK520 production. These results demonstrate that modifying genes within the biosynthetic gene clusters of natural products can be successfully applied to increase the yields of industrially and clinically important compounds. However, it is found that fkbR2 gene is a regulatory gene that has not been fully studied, and it is worth further studying its regulatory mechanism.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"493-507"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress.","authors":"Amel Harbaoui, Nadia Khelifi, Neyssene Aissaoui, Murielle Muzard, Agathe Martinez, Issam Smaali","doi":"10.1007/s00449-024-03120-2","DOIUrl":"10.1007/s00449-024-03120-2","url":null,"abstract":"<p><p>Cyanobacterial exopolysaccharides (EPS) remain released by cyanobacteria in the surrounding environment with the main purpose of protection against harmful environmental conditions. Recently, they have received significant attention due to their unique structural characteristics, functional properties, and potential applications across various fields. The current study describes the evaluation of EPS production under salinity stress from Arthrospira maxima. The application of high salinity up to 40 g/L enhanced EPS production, which was collected and purified by alcohol precipitation followed by membrane dialysis and lyophilization. A yield of 60 mg/L was obtained. The Size exclusion chromatography gave for the purified EPS an apparent molecular weight of 2.1 × 10⁵ Da. Monosaccharide composition showed that EPS is a heteropolymer, with mannose, xylose, and glucuronic acid identified as the predominant monosaccharides and derivatives. Nuclear magnetic resonance spectroscopy (¹³C and ¹H) confirmed that EPS is a heteropolysaccharide, entirely in α- anomeric configuration, with glucuronic acid as a main monomer that is probably linked to mannose and xylose via α-glycosidic linkages. Bioactivity assessment of EPS revealed that it exhibits antibacterial activity against several strains, notably, Bacillus subtilis (MIC: 0.6 ± 0.05 mg/mL), Bacillus cereus (MIC: 1 ± 0.01 mg/mL), Escherichia coli (MIC: 0.8 ± 0.01 mg/mL) and Klebsiella pneumonia (MIC: 0.8 ± 0.01 mg/mL). Antioxidant activity was measured using the DPPH radical scavenging assay, yielding an IC₅₀ of 6.83 mg/mL. Besides, EPS was also found to exhibit an interesting emulsifying property with several oil types, indicating its potential as a versatile biopolymer for applications in various industrial sectors.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"445-460"},"PeriodicalIF":3.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}