Bioprocess and Biosystems Engineering最新文献_第8页

High-yield soluble expression and affinity-tag-free purification of growth hormone receptor antagonist B2036 in Escherichia coli. 生长激素受体拮抗剂B2036在大肠杆菌中的高效可溶性表达及无亲和标签纯化。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-17 DOI: 10.1007/s00449-026-03388-6

Haoju Hua, Yapeng Wang, Junteng Feng, Yinuo Feng, Ying Zhang, Jianguang Lu, Jun Feng

{"title":"High-yield soluble expression and affinity-tag-free purification of growth hormone receptor antagonist B2036 in Escherichia coli.","authors":"Haoju Hua, Yapeng Wang, Junteng Feng, Yinuo Feng, Ying Zhang, Jianguang Lu, Jun Feng","doi":"10.1007/s00449-026-03388-6","DOIUrl":"10.1007/s00449-026-03388-6","url":null,"abstract":"Acromegaly is a severe endocrine disease, and growth hormone receptor antagonist B2036 constitutes the core protein backbone of pegvisomant. Conventional B2036 production based on inclusion bodies or periplasmic expression suffers from low yield, complicated refolding, and multi-step purification. Herein, we developed a highly efficient, refolding-free bioprocess for soluble expression of B2036 in Escherichia coli using SUMO fusion technology. The entire fermentation cycle was controlled within 24 h, and fermentation optimization via response surface methodology enabled a high-yield soluble yield of 0.95 g/L. For downstream processing, an industrial-scalable, affinity-tag-free strategy using only two chromatography steps (anion-exchange and mixed-mode chromatography) was established, producing 498.47 mg/L B2036 with 97.12% purity, representing a 20-fold improvement over previous reports. The obtained B2036 showed correct sequence, precise disulfide pairing, and strong biological activity with an IC₅₀ of 0.67 nmol/L. By removing protein refolding and metal-affinity chromatography, this simplified and robust workflow remarkably improves productivity and product quality, offering a cost-effective and scalable technical platform for large-scale manufacturing of pegvisomant biosimilars. Collectively, these findings provide a valuable reference for developing efficient soluble expression and downstream purification processes of other intrachain disulfide-bonded proteins in E. coli.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2225-2237"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application potential of a marine-derived halotolerant yeast in saline azo dye wastewater treatment: elucidating degradation pathways and osmo-adaptive strategies. 海洋耐盐酵母在含盐偶氮染料废水处理中的应用潜力：阐明降解途径和渗透适应策略。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-20 DOI: 10.1007/s00449-026-03396-6

Zhiyu Dong, Haiyun Zhang, Bingwen Xu, Xinmei Fu, Liang Tan

{"title":"Application potential of a marine-derived halotolerant yeast in saline azo dye wastewater treatment: elucidating degradation pathways and osmo-adaptive strategies.","authors":"Zhiyu Dong, Haiyun Zhang, Bingwen Xu, Xinmei Fu, Liang Tan","doi":"10.1007/s00449-026-03396-6","DOIUrl":"10.1007/s00449-026-03396-6","url":null,"abstract":"A halotolerant yeast strain designated ZY was isolated from marine sediment and identified as Candida tropicalis, in the present study. The strain demonstrated efficient decolorization of multiple azo dyes under high-salinity conditions, with NaCl concentrations ranging from 10.0 to 110.0 g/L. Through single-factor optimization, the growing cells of the yeast ZY achieved over 97% removal of the model azo dye Acid Red 3R (1600.0 µmol/L, approximately equivalent to 861.6 mg/L) within 12 h under the optimized conditions [sucrose 2.0 g/L, (NH4)2SO4 0.2 g/L, yeast extract 0.06 g/L, NaCl 30.0 g/L, 160 rpm, 35 °C, pH 6.0]. Acute toxicity assays indicated significant detoxification of the dye 3R after biodegradation. Activities of key enzymes, including NADH-DCIP reductase, lignin peroxidase, manganese peroxidase and laccase, were determined intracellularly. Metabolites and transcriptomics analysis revealed a proposed degradation pathway involving azo bond cleavage, hydroxylation, deamination, desulfurization, ring opening and ultimately mineralization via the TCA cycle. Comparative transcriptomics further revealed that the yeast ZY adapts to high salinity through cell wall remodeling, ion homeostasis, compatible solute accumulation and stress-responsive gene regulation. This study highlights the potential of the yeast ZY as a promising biocatalyst for treating saline azo dye wastewater.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2271-2290"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial fuel cell-assisted constructed wetlands for wastewater treatment and energy recovery: advances, performance trends, and future directions. 微生物燃料电池辅助人工湿地用于废水处理和能量回收：进展、性能趋势和未来方向。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-21 DOI: 10.1007/s00449-026-03391-x

Nidhi Ahlawat, Vishal Panghal, Pooja Choudhary, Annu Radha Nandal, Sandeep Kumar, Arup Giri, Sunil Kumar

{"title":"Microbial fuel cell-assisted constructed wetlands for wastewater treatment and energy recovery: advances, performance trends, and future directions.","authors":"Nidhi Ahlawat, Vishal Panghal, Pooja Choudhary, Annu Radha Nandal, Sandeep Kumar, Arup Giri, Sunil Kumar","doi":"10.1007/s00449-026-03391-x","DOIUrl":"10.1007/s00449-026-03391-x","url":null,"abstract":"The accelerating degradation of global water resources and the growing energy insecurity have intensified the demand for sustainable technologies that can simultaneously treat wastewater and generate energy. Constructed wetlands integrated with microbial fuel cells (CW-MFCs) have emerged as an innovative nature-based solution that synergistically combines ecological treatment processes with bioelectrochemical energy generation. This review summarizes recent advancements in CW-MFC technology, focusing on system mechanisms, structural configurations, operational parameters, and performance outcomes. A comprehensive bibliometric analysis was conducted to identify emerging trends, key developments, and knowledge gaps in CW-MFC research. A detailed comparative assessment of representative studies highlights the influence of wetland vegetation, electrode materials, microbial communities, pollutant removal efficiencies, and metrics related to bioelectricity generation. Multivariate bubble plots and treemap visualizations are employed to systematically demonstrate how variations in plant species, electrode configurations, and microbial assemblages affect chemical oxygen demand (COD) removal (60-95%) and voltage outputs (300-800 mV). Beyond conventional organic matter and nutrient removal, the applicability of CW-MFCs for treating emerging contaminants, including antibiotics, dyes, and heavy metals, is critically evaluated. Key technical, operational, and scalability challenges restricting large-scale deployment are identified, along with potential strategies for system optimization. CW-MFCs improve water quality by removing pollutants, provide a sustainable sanitation solution, and enable clean energy generation while reducing greenhouse gas emissions. These abilities of CW-MFC align with SDG 6 (Clean Water and Sanitation), 7 (Affordable and Clean Energy), and 13 (Climate Action).","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1993-2031"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate. 以废食用油为底物的PHA混合微生物培养的富集。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-09 DOI: 10.1007/s00449-026-03359-x

C Ucha, D Correa-Galeote, A Val Del Rio, A Pedrouso, A Mosquera-Corral

{"title":"Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate.","authors":"C Ucha, D Correa-Galeote, A Val Del Rio, A Pedrouso, A Mosquera-Corral","doi":"10.1007/s00449-026-03359-x","DOIUrl":"10.1007/s00449-026-03359-x","url":null,"abstract":"The synthesis of polyhydroxyalkanoates (PHA) by a mixed microbial culture (MMC) employing used cooking oil (UCO) as substrate represents a promising bioprocess for organic wastes valorisation. In this study, MMC enrichment in a sequencing batch reactor (SBR) was investigated using UCO without pretreatment as the sole carbon source. Two SBRs (SBR1 and SBR2) were operated under a feast-famine regime with uncoupled carbon and nitrogen supply, differing in the timing of the biomass withdrawal and nutrient filling. In SBR1, with effluent withdrawal at the end of the feast phase, better poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) accumulation was achieved (average values of 4.2 ± 1.6 wt. %, maximum of 19.9 wt. %) compared to SBR2, with withdrawal at the end of the famine phase (average values of 2.7 ± 1.1 wt. %, maximum of 5.2 wt. %). The highest PHBV accumulation in SBR1 was obtained at a COD/N ratio of 46 g/g, with an active biomass concentration of approximately 1 g VSS/L. Additionally, a single UCO pulse enhanced substrate hydrolysis and consumption compared to multiple pulses. Microbial community analysis revealed the enrichment of bacterial OTUs with PHA accumulation capacity related to Azospirillum, Acinetobacter, Gordonia, Burkholderia, and Bacillus. Among fungal OTUs, Geotrichum, Meyerozyma, and Pascua were the most abundant. Multivariate statistical analysis indicated that the synergistic interaction of Burkholderia and Chitinophaga played a crucial role in achieving the highest PHBV accumulation levels.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2045-2059"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13424269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of three inoculum sources for acetate production and microbial succession in H₂/CO₂-fed anaerobic system. 三种接种源对H2/ co2厌氧系统中乙酸生成及微生物演替的比较。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-02 DOI: 10.1007/s00449-026-03381-z

Nana Jiang, Linxiao Li, Xiyan Li, Ruixiang Li, Guoliang Wang, Tian Li, Xin Wang

{"title":"Comparison of three inoculum sources for acetate production and microbial succession in H2/CO2-fed anaerobic system.","authors":"Nana Jiang, Linxiao Li, Xiyan Li, Ruixiang Li, Guoliang Wang, Tian Li, Xin Wang","doi":"10.1007/s00449-026-03381-z","DOIUrl":"10.1007/s00449-026-03381-z","url":null,"abstract":"Introducing a synthetic H2/CO2 gas mixture into anaerobic systems is a promising strategy for C1 gas valorization, renewable energy storage, and sustainable chemical synthesis. However, systematic comparisons of inoculum sources under identical operational conditions remain limited. In this study, three inoculum sources, fermentation sludge (S1), lake sediment (S2), and brewery anaerobic sludge (S3), were compared for acetate production from CO2 and H2, with particular attention to microbial community succession and reactor stability. S3 showed the highest CO2 consumption rate (331 ± 4 mg CO2/(L·d)), cumulative CO2 fixation (7.8 ± 0.3 g/L), and acetate production efficiency (5.3% electron allocation to acetate), along with stable pH conditions (7.1 ± 0.3). These outcomes were associated with the rapid enrichment of genera that include known homoacetogenic species, mainly Clostridium and Acetobacterium, which together reached approximately 40% relative abundance in S3 after acclimation. The findings suggest that initial inoculum composition can influence the extent of homoacetogen enrichment and subsequent acetate production performance. In addition, the post-peak increase in the acetate-oxidizing genus Geobacter (an acetate consumer rather than an acetogen) in S3 points to a potential challenge for sustained product recovery in mixed-culture systems. Overall, these results provide insights for inoculum selection and process optimization in H2/CO2 fermentation systems for acetate production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2133-2145"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving trehalose production through the design and optimization of linker peptides in scaffold protein. 通过支架蛋白连接肽的设计和优化来提高海藻糖的产量。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-03 DOI: 10.1007/s00449-026-03385-9

Changtong Chen, Jiachen An, Haibo Yuan, Di Huang, Yi Jiang, Hongling Liu, Tengfei Wang

{"title":"Improving trehalose production through the design and optimization of linker peptides in scaffold protein.","authors":"Changtong Chen, Jiachen An, Haibo Yuan, Di Huang, Yi Jiang, Hongling Liu, Tengfei Wang","doi":"10.1007/s00449-026-03385-9","DOIUrl":"10.1007/s00449-026-03385-9","url":null,"abstract":"Trehalose is an excellent bioprotective agent with substantial applications in biomedicine, cosmetics, and food. Currently, trehalose production utilizes a bi-enzyme method involving free maltooligosyltrehalose synthase (MTSase) and maltooligosyltrehalose trehalohydrolase (MTHase). The spatial distance between the active sites of the two enzymes can be shortened by assembling a bi-enzyme complex using a scaffold protein to improve catalytic efficiency. In this study, previously constructed MTSase-CcDoc and CtDoc-MTHase fusion enzymes were combined with a scaffold protein to form a bi-enzyme complex via Cohesin-Dockerin interaction to catalyze trehalose production using maltodextrin as a substrate. By designing the structural length and rigidity of the 12 linker peptides to replace the native linker peptide in the scaffold protein, trehalose production was increased by 1.24 and 1.17 times compared with that using free enzymes and the native linker peptides, respectively. Predictive models of enzyme binding sites, along with structural and docking analyses, further confirmed the enhanced catalytic activity and structural integrity of the bi-enzyme complex, providing a foundation for developing efficient trehalose synthesis pathways.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2185-2197"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A computational framework for building dynamic bioreactor models integrating detailed enzyme kinetics with limited data: beta-ionone production in Saccharomyces cerevisiae as a case study. 构建动态生物反应器模型的计算框架，将详细的酶动力学与有限的数据相结合：以酿酒酵母的β -离子酮生产为例研究。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-10 DOI: 10.1007/s00449-026-03384-w

Alejandro I Sepúlveda-Celis, Eduardo Agosin, José R Pérez-Correa, Pedro A Saa

{"title":"A computational framework for building dynamic bioreactor models integrating detailed enzyme kinetics with limited data: beta-ionone production in Saccharomyces cerevisiae as a case study.","authors":"Alejandro I Sepúlveda-Celis, Eduardo Agosin, José R Pérez-Correa, Pedro A Saa","doi":"10.1007/s00449-026-03384-w","DOIUrl":"10.1007/s00449-026-03384-w","url":null,"abstract":"Kinetic models are useful tools for predicting the dynamic behavior of metabolic systems and to optimize the performance of bioprocesses. However, the difficulty of fitting their parameters to limited data hinders their widespread use. These models have various disparate parameters, are nonlinear, and display complex interactions. To address this challenge, this study introduces a computational framework for constructing bioreactor models integrating detailed enzyme kinetics under data-limited conditions. The framework is illustrated by the construction of a dynamic model that describes the growth kinetics of Saccharomyces cerevisiae and the production of β-ionone, an apocarotenoid extensively utilized in the flavor and fragrance industries, in batch cultivations. The model was initially formulated and described using 78 free kinetic parameters. Through the systematic application of sensitivity and identifiability analyses and reparameterization, the model was reduced to 9-parameter candidate structures. Multi-criteria decision-making techniques were then employed to select a robust model structure. When validated against an independent experimental dataset with condition-specific cofactor adjustment, the final model achieved comparable overall predictive performance compared to the original model structure, with notable improvements for key pathway metabolites, including up to 38% reduction in the normalized mean absolute error for the target product β-ionone. This framework successfully addresses the challenge of constructing predictive kinetic models from limited experiments while maintaining mechanistic interpretability, providing a quantitative tool for identifying metabolic bottlenecks and guiding metabolic engineering interventions.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2163-2183"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pichia pastoris-based platform for large-scale production of functional recombinant human serum albumin with therapeutic efficacy. 基于毕赤酵母的大规模生产具有治疗效果的功能性重组人血清白蛋白的平台。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-13 DOI: 10.1007/s00449-026-03387-7

Lijiong Li, Jing Lin, Mengfa Cui, Hailong Fu, Zhilin Wang

{"title":"A Pichia pastoris-based platform for large-scale production of functional recombinant human serum albumin with therapeutic efficacy.","authors":"Lijiong Li, Jing Lin, Mengfa Cui, Hailong Fu, Zhilin Wang","doi":"10.1007/s00449-026-03387-7","DOIUrl":"10.1007/s00449-026-03387-7","url":null,"abstract":"Human serum albumin (HSA) is critical for treating liver cirrhosis with ascites. However, plasma-derived HSA (pHSA) faces limitations in supply and carries potential infection risks. To address the urgent need for a therapeutically equivalent alternative, we established an efficient expression system for recombinant HSA (rHSA) in Pichia pastoris. We purified rHSA via a three-step chromatography purification process, yielding approximately 260 mg per 1.5 L of culture. Comprehensive analyses confirmed its structural equivalence to pHSA. Mass spectrometry revealed a molecular weight of 66,530.8 Da, while circular dichroism showed a 36.9% α-helical content. Furthermore, isothermal titration calorimetry demonstrated comparable drug-binding affinities for naproxen and warfarin. In a CCl₄-induced rat model of liver cirrhosis with ascites, rHSA displayed significant, dose-dependent therapeutic effects. Notably, high-dose rHSA achieved efficacy statistically equivalent to pHSA. It reduced ascites volume by 50.1%, improved liver index by 33.5% and restored colloid osmotic pressure along with liver function markers (ALT, AST, and bilirubin). This study provides the first in vivo evidence confirming the therapeutic equivalence and dose-effect relationship of P. pastoris-derived rHSA compared with pHSA. These findings establish a scalable, cost-effective, and safe platform for rHSA production, supporting its potential as a viable clinical substitute for plasma-derived albumin.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2213-2223"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative fermentability of a glucose/xylose enriched enzymatic slurry using different yeast strains. 不同酵母菌株对葡萄糖/木糖富集酶浆发酵性能的比较。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-24 DOI: 10.1007/s00449-026-03399-3

Lina M Durán, Miguel A D Flores-Alarcón, Adriane M F Milagres, Inês C Roberto

{"title":"Comparative fermentability of a glucose/xylose enriched enzymatic slurry using different yeast strains.","authors":"Lina M Durán, Miguel A D Flores-Alarcón, Adriane M F Milagres, Inês C Roberto","doi":"10.1007/s00449-026-03399-3","DOIUrl":"10.1007/s00449-026-03399-3","url":null,"abstract":"Lignocellulosic biomass represents a sustainable energy source as ethanol fuel demand increases; process integration and efficient C6/C5 sugars utilization are essential for achieving techno-economically viable production. Here, alkaline-sulfite pretreatment combined with disc refining was applied to sugarcane bagasse for conserving most of cellulose and hemicellulose fractions. Then, β-glucosidase supplementation of cellulase at rational enzyme dosage (18 FPU/g biomass) was established for the enzymatic hydrolysis of the pretreated solids, achieving approximately 75% conversion yield for both cellulose and xylan. The resulting enzymatic slurry was directly fermented without solid-liquid separation and used to evaluate the effects of temperature and nutrient supplementation on ethanol fermentation by three yeast strains. Under optimal conditions (30 °C with nutrients), Kluyveromyces marxianus Y-6860 exhibited higher ethanol productivity ([Formula: see text] = 3.25 g L- 1 h- 1) than Saccharomyces cerevisiae PE-2 ([Formula: see text] = 2.17 g L- 1 h- 1). Notably, Scheffersomyces stipitis Y-7124 was the only strain capable of significantly consuming xylose, resulting in 46% higher ethanol production than the other strains. To simulate industrially relevant conditions, the slurry was supplemented with anhydrous glucose up to 100.0 g L- 1. Under these conditions, K. marxianus Y-6860 and S. cerevisiae PE-2 maintained robust fermentation performance, while S. stipitis Y-7124 exhibited limited xylose uptake during the evaluated period. The results demonstrate the potential of direct slurry fermentation for integrated 2G ethanol production, with K. marxianus Y-6860 showing strong potential for industrial-scale glucose fermentation and S. stipitis Y-7124 being a prospective candidate for improving xylose conversion.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2303-2315"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13424243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148577085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step immobilization of mutant TvDAAO yields an active and stable biocatalyst in extreme conditions. 突变体TvDAAO的一步固定化在极端条件下产生了活性和稳定的生物催化剂。

IF 4.3 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-13 DOI: 10.1007/s00449-026-03386-8

Denis L Atroshenko, Maria A Vlasova, Mikhail D Shelomov, Anastasia A Pometun, Svyatoslav S Savin, Vladimir I Tishkov

{"title":"One-step immobilization of mutant TvDAAO yields an active and stable biocatalyst in extreme conditions.","authors":"Denis L Atroshenko, Maria A Vlasova, Mikhail D Shelomov, Anastasia A Pometun, Svyatoslav S Savin, Vladimir I Tishkov","doi":"10.1007/s00449-026-03386-8","DOIUrl":"10.1007/s00449-026-03386-8","url":null,"abstract":"D-Amino acid oxidase (DAAO) is an important enzyme in modern biotechnology, used for synthetic, analytical, and medical purposes. Numerous mutant forms of DAAO with different properties have been described previously. Recently, we combined six beneficial amino acid substitutions in DAAO from Trigonopsis variabilis (TvDAAO) to create the multipoint mutant TvDAAO E32R/F33D/F54S/C108F/M156L/C298N (mut-TvDAAO). Compared to the wild-type TvDAAO, the new mutant enzyme showed a fourfold higher catalytic constant in the oxidation of cephalosporin C, an eightfold higher stability against hydrogen peroxide oxidation, and a twentyfold greater thermal stability. In the present work, we demonstrate a one-step immobilization procedure of mut-TvDAAO on strong anion exchange beads Sepabeads EC-QA, yielding an immobilized biocatalyst with enhanced resistance to thermal, oxidative, alkaline, and aeration-induced stress. After immobilization, mut-TvDAAO retains approximately 40% of its initial activity even after incubation for 5 h at 90 °C. Immobilized mut-TvDAAO (imm-mut-TvDAAO) retains approximately 20% of its initial activity in the presence of 0.1 M hydrogen peroxide after 5 h of incubation. The immobilized enzyme remains fully active after 5 h of incubation at pH 13.0. Importantly, immobilization improves catalyst performance under intensive oxygen aeration required for cephalosporin C oxidation, and the activity of imm-mut-TvDAAO does not decrease over 10 consecutive cycles of use. These results show that simple carrier-based immobilization of an engineered TvDAAO variant provides an operationally robust biocatalyst for oxidative transformations under process-relevant stress conditions.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2199-2211"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0