Visualization of fluorescently labeled lipase distribution characteristics at the oil-water interface.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-04-03 DOI:10.1007/s00449-025-03157-x

Xian Du, Chunxiao Zhang, Biyu Peng

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引用次数: 0

Abstract

A method based on fluorescently labeled enzyme proteins was established to visualize the absorption properties of lipase at the oil-water interface, and it can be used for the effective observation of the distribution characteristics of lipase at the oil-water interface. The optimal conditions for observation include the following: oil content of 10-20% (wt%), concentration of fluorescently labelled enzyme protein of 0.25 mg/mL, reaction temperature of 25-30 °C, emulsion dispersion and stirring time of 10 min, and emulsion resting time of 30-120 s. Based on this method, a preliminary analysis of the effects of oil and lipase species on the distribution characteristics of lipase at the oil-water interface was performed. The results reveal that differences in the distributions of lipase at the oil-water interface of various fats and oils had a certain degree of correspondence with their specificity and that the distribution characteristics of the lipases on the surface of olive oil enabled effective catalytic hydrolysis to a certain extent. This method is a more objective guide for the development of lipase application technology in the fields of tanning, fur making, glue making, detergents, and sewage treatment and so on.

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油水界面荧光标记脂肪酶分布特征可视化。

建立了一种基于荧光标记酶蛋白的油水界面吸附特性可视化方法，可用于有效观察油水界面上脂肪酶的分布特征。最佳观察条件为：油脂含量10-20% (wt%)，荧光标记酶蛋白浓度0.25 mg/mL，反应温度25-30℃，乳剂分散搅拌时间10 min，乳剂静置时间30-120 s。在此基础上，初步分析了油脂和脂肪酶种类对油水界面脂肪酶分布特性的影响。结果表明，不同油脂在油水界面处脂肪酶分布的差异与其特异性有一定的对应关系，而脂肪酶在橄榄油表面的分布特征在一定程度上促成了有效的催化水解。该方法为脂肪酶在制革、制毛、制胶、洗涤剂、污水处理等领域应用技术的发展提供了较为客观的指导。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.