Bioprocess and Biosystems Engineering最新文献

One-pot bioprocessing: dynamics and opportunities for integrated multiproduct recovery-a review. 一锅生物处理：多产品综合回收的动态与机遇综述。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-05-08 DOI: 10.1007/s00449-026-03341-7

Daniel O Ojwang, Sammy K Chebon, Francis J Mulaa

{"title":"One-pot bioprocessing: dynamics and opportunities for integrated multiproduct recovery-a review.","authors":"Daniel O Ojwang, Sammy K Chebon, Francis J Mulaa","doi":"10.1007/s00449-026-03341-7","DOIUrl":"https://doi.org/10.1007/s00449-026-03341-7","url":null,"abstract":"One-pot bioprocessing (OPB) represents an integrated strategy for biomass valorization within a single reactor rather than across sequential, isolated units. By consolidating formation-stage steps that are traditionally separated, OPB can lower capital intensity and reduce intermediate losses. It may also improve carbon utilization under specific conditions compared with conventional modular biorefineries optimized around a single product. Despite these advantages, OPB has yet to achieve robust scalability. This review examines the dynamic processes governing biomass fractionation into individual constituents and biocatalytic transformation in single-reactor systems. It synthesizes recent advances in metabolic engineering, process intensification, and dynamic flux control to assess how biological network behavior, thermodynamic feasibility, and reactor-scale transport phenomena jointly constrain feasible product combinations within integrated one-pot systems. Persistent limitations arising from metabolic trade-offs, physicochemical incompatibilities, and increasing control complexity are evaluated alongside emerging enabling strategies, including dynamic metabolic regulation, hybrid one-pot architectures, and digital bioprocess twins. This work provides a data-informed framework indicating that effective one-pot bioprocess design depends on aligning biological compatibility, control capacity, and operational robustness to support adaptive and anticipatory control of multiproduct formation dynamics.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing strategies for high-level production of peptide-N-glycosidase F (PNGase F) from Flavobacterium meningosepticum using high cell density fed-batch culture of E. coli. 大肠杆菌高密度补料分批培养脑膜败血症黄杆菌高产肽- n -糖苷酶F （PNGase F）的研究

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-05-02 DOI: 10.1007/s00449-026-03337-3

Shilpa Mohanty, Babbal, Shivani Chauhan, Mohini Talwar, Shubham Sharma, Priya Maravi, Yogender Pal Khasa

{"title":"Designing strategies for high-level production of peptide-N-glycosidase F (PNGase F) from Flavobacterium meningosepticum using high cell density fed-batch culture of E. coli.","authors":"Shilpa Mohanty, Babbal, Shivani Chauhan, Mohini Talwar, Shubham Sharma, Priya Maravi, Yogender Pal Khasa","doi":"10.1007/s00449-026-03337-3","DOIUrl":"https://doi.org/10.1007/s00449-026-03337-3","url":null,"abstract":"Protein N-glycosylation is a crucial post-translational modification that regulates cellular processes such as cell signalling, development, and autophagy. Abnormalities in protein glycosylation can manifest in life-threatening conditions, such as cancer. N-glycan analysis is crucial for determining the underlying cause of disease. The characterization of N-linked glycans is achieved through the removal of the carbohydrate moiety using deglycosylating enzymes. Peptide-N-glycosidase F (PNGase F) is a glycoamidase that hydrolyzes the amide bond in glycosamide by specifically cleaving at the innermost N-acetylglucosamine (GlcNAc). Although PNGase F has significant therapeutic applications, its widespread commercial use is limited by its high cost. This study focused on heterologous expression of Flavobacterium meningosepticum PNGase F in E. coli BL21 (DE3) and SHuffle® cells, in which the protein was partially soluble. The E. coli SHuffle® cells yielded 210.41 mg/L of PNGase F in TB glycerol medium in shake flasks, with a corresponding YP/X of 47.20 mg/g DCW. Expression studies in E. coli BL21 (DE3) cells yielded inclusion bodies (IBs) that were solubilized, yielding activity comparable to that of the soluble form. Furthermore, the fed-batch cultivation in E. coli BL21 (DE3) cells produced 5.87 g/L of IBs with a final OD600 of 176. Therefore, this study investigates the potential of alternative E. coli hosts as cost-effective production platforms for PNGase F.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Study of photocatalytic activity of green synthesized nickel oxide nanoparticles in the degradation of acid orange 7 dye under visible light. 论文摘要：绿色合成氧化镍纳米颗粒在可见光下降解酸性橙7染料的光催化活性研究。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-30 DOI: 10.1007/s00449-026-03348-0

Khadijeh Hamidian, Amir Hossein Rigi, Ahmad Najafidoust, Mina Sarani, Abdolhossein Miri

引用次数: 0

Purifae - a modular bioreactor for microalgae culture made of biopolymer using additive techniques. Purifae -一种模块化的微藻培养生物反应器，由生物聚合物采用添加剂技术制成。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-29 DOI: 10.1007/s00449-026-03332-8

Olimpia Wojciechowska, Adam Grzywaczyk, Zuzanna Styrna, Maciej Staszak, Wojciech Smułek

引用次数: 0

Fermentative routes of microbial biosurfactants production: emerging trends, innovations and multifaceted applications. 微生物生物表面活性剂生产的发酵途径：新趋势、创新和多方面应用。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-29 DOI: 10.1007/s00449-026-03321-x

Debraj Maji, Dheepak Ravichandran, Garima Dalal, Kanhaiya Kumar

引用次数: 0

Application of magnetic fields to the microalgal cultivation of Nannochloropsis oceanica. 磁场在海洋纳米绿藻微藻培养中的应用

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-29 DOI: 10.1007/s00449-026-03339-1

Danielle Barros Urrutia, Bruno Roswag Machado, Pedro Senger de Souza, Carlos Rafael Borges Mendes, Fabio Roselet, Lucielen Oliveira Santos

引用次数: 0

Design and validation of an automated low-cost system for real-time gas quantification in bioreactors using pressure sensors. 使用压力传感器的生物反应器实时气体定量自动化低成本系统的设计和验证。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-18 DOI: 10.1007/s00449-026-03334-6

Rodrigo Santos Souza, Alessandra Giordani, Rafael Brito de Moura, Laos Alexandre Hirano, Rafael de Oliveira Tiezzi

引用次数: 0

Integrating image processing and machine learning to enhance microalgal nitrate removal via optimized CO₂ holdup and pH-DO dynamics. 整合图像处理和机器学习，通过优化CO2含率和pH-DO动态来增强微藻硝酸盐去除。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-18 DOI: 10.1007/s00449-026-03305-x

Fariba Rezvani, Seyedehsamaneh Shojaeilangari, Esmat Kishani Farahani

引用次数: 0

Biodegradation of triclosan under non-sterile conditions by co-culture of Bacillus licheniformis and Lysinibacillus fusiformis. 非无菌条件下地衣芽孢杆菌与梭状芽孢杆菌共培养降解三氯生的研究。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-18 DOI: 10.1007/s00449-026-03331-9

Elanur Dasdemir, Mesut Taskin, Hakan Ozkan

引用次数: 0

Soybean molasses: a promising substrate for the production of microbial oil by Phaffia rhodozyma. 大豆糖蜜：法菲酵母生产微生物油的一种有前途的底物。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-15 DOI: 10.1007/s00449-026-03312-y

Shirley Denisse Ccori Ponce, Luiz Henrique Han, Luiz Antonio de Almeida Pinto, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros Burkert

{"title":"Soybean molasses: a promising substrate for the production of microbial oil by Phaffia rhodozyma.","authors":"Shirley Denisse Ccori Ponce, Luiz Henrique Han, Luiz Antonio de Almeida Pinto, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros Burkert","doi":"10.1007/s00449-026-03312-y","DOIUrl":"https://doi.org/10.1007/s00449-026-03312-y","url":null,"abstract":"Soybean molasses represents a promising substrate for the production of high-value bioproducts, particularly in the cultivation of Phaffia rhodozyma, a yeast classified as GRAS (Generally Recognized as Safe). Although this yeast is well known for carotenoid synthesis, its potential for lipid accumulation remains largely unexplored. In this study, oil production by P. rhodozyma was evaluated using soybean molasses as the sole carbon source. The yeast was cultured in a 5 L bench-scale bioreactor (100 g·L⁻¹ substrate, 300 rpm, 1 vvm, 25°C) and the biomass was subsequently spouted bed drying. Lipids were extracted following the Bligh and Dyer method. After 144 h, 12.59 g.L⁻¹ of biomass was obtained, containing 154.23 µg·g⁻¹ of astaxanthin and 85.91 µg·g⁻¹ of β-carotene. Lipid yield reached 34.9% (w.w- 1), corresponding to a C/N ratio of 44.39 in the cultivation medium. Fatty acid analysis revealed a predominance of oleic acid (66.98%), with a saturated/unsaturated ratio of 0.24, iodine value of 74 g.I2.100 g⁻¹, saponification value of 161 mg KOH.g⁻¹, acidity of 5.02 mg KOH.g⁻¹, and peroxide value of 3.19 mEq O₂.kg⁻¹. These results demonstrate the potential of P. rhodozyma cultivated on soybean molasses to produce microbial oil with high nutritional and functional value, favorable lipid composition, and oxidative stability, highlighting the need for optimization strategies in the oil recovery step to enable future applications in the food industry.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147688111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0