Bioprocess and Biosystems Engineering最新文献

{"title":"High-pressure treatment of the green and orange Dunaliella salina biomass: effect on particle size distribution, small amplitude oscillatory shear rheology, and microstructure.","authors":"Jasim Ahmed, Vanita Vinod Kumar, Vinod Kumar, Sabah AlMomin","doi":"10.1007/s00449-025-03160-2","DOIUrl":"10.1007/s00449-025-03160-2","url":null,"abstract":"<p><p>Dunaliella salina, a halophilic microalga, is well known for its ability to produce β-carotene and has significant commercial applications. The actively growing green culture turns to orange color due to photosensitization, during which there is a significant reduction in chlorophyll content (chlorophyll A and B: 16.04 and 2.80-1.70, 0.21 mg/g dry basis, respectively) with an increase in carotenoids (α- and β-carotenes: 1.60 and 4.81 mg/g dry basis). This change has been accompanied by a considerable variation in protein content (green: 34.27% and orange: 18.57%) and ash content (green: 38.37% and orange: 58.11%). To avoid extreme heat sensitivity, high-pressure (HP) processing, a nonthermal technology, has been applied to pigment-rich Dunaliella. This research aimed to examine the effects of HP treatment (300-600 MPa/15 min) on the rheological, structural, and particle size distribution of Dunaliella in two consecutive cell growth stages (e.g., green and orange). Oscillatory rheology data displayed a distinct protein denaturation at 57.87 °C for untreated green cells, whereas orange cells did not. Conversely, several denaturation peaks appeared in the HP-treated orange cell suspensions, and those peaks remained unaffected by pressure treatment. Isothermal heating exhibited liquid-like behavior for green cells, whereas the solid-like behavior was evident for orange cells. PSD displayed a shift of unimodal to bimodal distributions of Dunaliella cells after the HP treatment. Orange cells exhibited PSD parameters of Dv₁₀: 8.60 μm, Dv₅₀: 71.6 μm, and Dv₉₀: 255 μm. XRD patterns of both green and orange cells are almost identical, exhibiting several peaks that were attributed to metal ions absorbed by the cells from the growth media. Overall, a significant difference in compositional and functional properties was observed between the green and orange Dunaliella biomass.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1025-1037"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development.","authors":"Clara Lüchtrath, Eva Forsten, Romeos Polis, Maximilian Hoffmann, Aylin Sara Genis, Anna-Lena Kuhn, Marcel Hövels, Uwe Deppenmeier, Jørgen Magnus, Jochen Büchs","doi":"10.1007/s00449-025-03159-9","DOIUrl":"10.1007/s00449-025-03159-9","url":null,"abstract":"<p><p>Vibrio natriegens is a fast-growing microbial workhorse with high potential for biotechnological applications. However, handling the bacterium in batch processes is challenging due to its high overflow metabolism and mixed acid formation under microaerobic conditions. For early process development, technologies enabling small-scale fed-batch cultivation of V. natriegens Vmax are needed. In this study, fed-batch cultivations in 96-well microtiter plates were successfully online-monitored for the first time with a µTOM device. Using the online-monitored oxygen transfer rate, a scale up to membrane-based fed-batch shake flasks was performed. The overflow metabolism was efficiently minimized by choosing suitable feed rates, and mixed acid formation was prevented. A glucose soft sensor using the oxygen transfer rate provided accurate estimates of glucose consumption throughout the fermentation, eliminating the need for offline sampling. Analyzing the impact of the inducer IPTG on the recombinant production of the enzyme inulosucrase revealed concentration-dependent effects in batch processes. In contrast, fed-batch operating mode resulted in high inulosucrase activity even without induction. Overall, an inulosucrase titer of 80 U/mL was achieved. In conclusion, the advantages of small-scale fed-batch technologies supported by a glucose soft sensor have been demonstrated for early process development for V. natriegens Vmax.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1007-1024"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualization of fluorescently labeled lipase distribution characteristics at the oil-water interface.","authors":"Xian Du, Chunxiao Zhang, Biyu Peng","doi":"10.1007/s00449-025-03157-x","DOIUrl":"10.1007/s00449-025-03157-x","url":null,"abstract":"<p><p>A method based on fluorescently labeled enzyme proteins was established to visualize the absorption properties of lipase at the oil-water interface, and it can be used for the effective observation of the distribution characteristics of lipase at the oil-water interface. The optimal conditions for observation include the following: oil content of 10-20% (wt%), concentration of fluorescently labelled enzyme protein of 0.25 mg/mL, reaction temperature of 25-30 °C, emulsion dispersion and stirring time of 10 min, and emulsion resting time of 30-120 s. Based on this method, a preliminary analysis of the effects of oil and lipase species on the distribution characteristics of lipase at the oil-water interface was performed. The results reveal that differences in the distributions of lipase at the oil-water interface of various fats and oils had a certain degree of correspondence with their specificity and that the distribution characteristics of the lipases on the surface of olive oil enabled effective catalytic hydrolysis to a certain extent. This method is a more objective guide for the development of lipase application technology in the fields of tanning, fur making, glue making, detergents, and sewage treatment and so on.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"981-992"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the pretreatment and hydrolysis of a mixture of coffee husk, cowpea bean husk and cocoa pod for bacterial cellulose production.","authors":"Kátia Dos Santos Morais, Ederson Paulo Xavier Guilherme, Bruna Dos Santos Menezes, Marcus Bruno Soares Forte, Patrícia Lopes Leal, Elizama Aguiar-Oliveira","doi":"10.1007/s00449-025-03158-w","DOIUrl":"10.1007/s00449-025-03158-w","url":null,"abstract":"<p><p>Agro-industrial solid residues (AISR) need to be valued as rich sources of nutrients and energy. This work aimed to obtain reducing sugars (RS) from a mixture of coffee husk (CF), cowpea bean husk (BE) and cocoa pod (CO) to produce bacterial cellulose (BC), a versatile alternative to plant cellulose. The most adequate conditions for pretreatment followed by enzymatic hydrolysis were selected with the Design of Experiments statistical tool for, respectively, a first solid load of 10% (w/v) of a mixture of CF:BE:CO = 2:8:2 in 0.25% (v/v) H₂SO₄, followed by a second solid load of 20% (w/v) in sodium citrate buffer [pH = 4.8 and] with 10 FPU/mg of cellulases. A hydrolysate was obtained, after 48 h of hydrolysis, containing 54.45 ± 2.61% (w/w) of glucose with a cellulose digestibility of almost 87%. This hydrolysate was added to nutrients and 20 g of pure glucose and was used in the cultivation of Komagataeibacter hansenii ATCC 23769 and resulted in BC = 179.00 ± 33.95 g/L (w. b.). These results encourage the biotechnological use of different AISR in mixtures to produce RS in order to obtain valuable materials, such as BC.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"993-1006"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling up biofilm bioreactors for enhanced menaquinone-7 production.","authors":"Aydin Berenjian, Ehsan Mahdinia, Ali Demirci","doi":"10.1007/s00449-025-03155-z","DOIUrl":"10.1007/s00449-025-03155-z","url":null,"abstract":"<p><p>The health benefits of menaquinone-7 (MK-7) are well-established, and its production through fermentation techniques is widespread. Our team developed an innovative biofilm reactor utilizing Bacillus subtilis natto cells to foster biofilm growth on plastic composite supports to produce MK-7. The scalability of this biofilm reactor from a 2-L benchtop scale in our laboratory and its potential for commercial applications pose significant unresolved questions. Therefore, the current research was aimed to scale up the biofilm reactor from bench scale (2-L) to the pilot scale (30-L) bioreactor. Three strategies were evaluated to understand their impact on MK-7 biosynthesis during bioreactor volume expansion: volumetric oxygen mass transfer coefficient (k_La), agitation power input per unit volume (P/V), and impeller tip velocity (V_tip). While k_La was successfully maintained during scaling, P/V and V_tip varied and were assessed for their influence on MK-7 production. After investigating these methods, it was found that the volumetric oxygen mass transfer coefficient (k_La) constant method proved to be the most effective one. The optimum MK-7 concentration achieved was 21.0 ± 1.0 mg/L, comparable to the highest MK-7 concentration of 20.6 ± 1.0 attained at the 2-L scale. This showcases the scalability of biofilm bioreactor technology and its promising potential for commercial production of MK-7. Furthermore, we explored the potential of fed-batch glucose addition to the base media in the biofilm reactor to enhance MK-7 concentration at the 30-L scale. Remarkably, results demonstrated that fed-batch strategy significantly increased MK-7 concentrations to 28.7 ± 0.3 mg/L, which made it almost 2.3-fold higher than levels produced in suspended-cell bioreactors. This finding highlights the potential of biofilm reactors as a promising replacement to the current static fermentation strategies for commercial production of MK-7.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"971-979"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of carbonyl reductase based on substrate pocket loop regions alteration: an application for synthesis of duloxetine chiral intermediate in high efficiency.","authors":"Xiao-Jian Zhang, Kai-Li Chen, Xiang-Yang Li, Qi-Qi Yuan, Chao-Ping Lin, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1007/s00449-025-03152-2","DOIUrl":"10.1007/s00449-025-03152-2","url":null,"abstract":"<p><p>Duloxetine, a prominent 5-hydroxytryptamine norepinephrine reuptake inhibitor, is deployed mainly in the management of adult depression, showcasing minimal side effects, swift therapeutic onset, and a robust safety profile. Ethyl (S)-3-hydroxy-3-(2-thienyl)propionate ((S)-HEES) is the crucial chiral intermediate for duloxetine production. Asymmetric synthesis of (S)-HEES using carbonyl reductase as the biocatalyst has exhibited advantages including mild reaction conditions, high catalytic efficiency and environmental friendliness. In the present study, a loop region alteration strategy was developed to screen for a carbonyl reductase for (S)-HEES synthesis and EaSDR6 from Exiguobacterium sp. s126 was identified with considerable catalytic performance and broad substrate adaptability. Site-directed mutagenesis was subsequently performed, Mut-R142A/N204A was identified with a 3.6-fold enhancement in activity relative to the wild-type EaSDR6. The mutant k_cat value was 52.5 s^-1, 2.9-fold compared to the wild type, and the total catalytic efficiency (k_cat/K_M) was 24.9 mM^-1 s^-1, 1.9-fold higher than the wild type. The n-butyl acetate-aqueous biphasic bioreaction system was established for the asymmetric synthesis of (S)-HEES with the conversion of ethyl 3-oxo-3-(2-thienyl)propionate (KEES) of 90.2%, the product e.e. of > 99% after 8 h reaction at a substrate concentration of 200 g/L. The spatiotemporal yield reached 22.5 g/(L·h), which was higher than the ever reports about (S)-HEES biosynthesis. The present research provides new knowledge and technology for the construction of stereoselective carbonyl reductase and the green biosynthesis of chiral alcohol pharmaceutical intermediates.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"927-937"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-assisted eco-benevolent synthesis of oxidase-mimic Cu-Mn₃O₄ as an antibacterial and antiproliferative agent.","authors":"Ankita Shome, Salim Ali, Debadrita Roy, Sangita Dey, Shilpa Sinha, Partha Barman, Anoop Kumar, Ranadhir Chakroborty, Md Salman Haydar, Swarnendu Roy, Shibaji Ghosh, Mahendra Nath Roy","doi":"10.1007/s00449-025-03149-x","DOIUrl":"10.1007/s00449-025-03149-x","url":null,"abstract":"<p><p>In recent years, the marked augment of antibiotic resistance hampered the development of antibacterial agent. Nanozymes by their in situ ROS production capability oxidize cellular substances of bacterial cell and eliminate MDR bacteria. Therefore, synthesis of effective nanozymes from green precursors is rarely reported, so the prime objective of this study was to synthesize Cu-Mn₃O₄ nanozymes from aqueous extracts of medicinal plant Azadirachta indica via co-precipitation approach and to endorse their biomedical applications. The synthesized materials were characterized by X-ray diffraction (XRD), Fourier Transform Infrared spectrometer (FTIR), Scanning Electron Images (SEM), and Field-Emission Scanning Electron Microscopy (FESEM) images. X-ray Diffraction (XRD) patterns revealed the formation of hausmannite Mn₃O₄ crystal system. Fourier Transform Infrared spectrometer (FTIR) spectra revealed functional groups on the surface nanoparticles for their stabilization. Energy-Dispersive X-ray spectroscopy (EDAX) profile confirmed the existence of desired elements in the synthesized nanozymes. B1 mimics oxidase enzyme most effectively with K_m = 0.175 mM and V_max = 10.34 µM/min. The low K_m and high V_max indicates the strong binding affinity and high catalytic activity. From the agar diffusion antibacterial assay, it can be concluded that B3 is the most potent antibacterial agent specifically against Gram-positive bacteria Bacillus subtilis with inhibition zone of 27 mm at 250 µg/mL. Their cytotoxic activities on neuroblastoma (SHSY5) cell line were investigated for the first time. The data revealed that synthesized nanooctahedrons possess a significant cytotoxicity against cancer cell lines SHSY5Y (IC₅₀ = 137.47 ± 14.11 µg/mL) and SKOV3 (IC₅₀ = 72.72 ± 9.33 µg/mL). Overall, with increasing Cu amount, the percentage growth inhibition of Mn₃O₄ crystal system enhanced. The improved antibacterial activity and cytotoxicity is due to synergy between metal and phytochemicals. Radical scavenging activity of synthesized nanozymes is comparatively lower than their green source and the comparatively lower IC₅₀ values of B1, 234.12 ± 15.13 and 220.12 ± 10.37 respectively, which indicates that it is more active in scavenging DPPH and ABTS radical. B2 (IC₅₀ = 310.56 ± 5.92 µg/mL) and B3 (IC₅₀ = 43.56 ± 3.03 µg/mL) scavenge superoxide radicals and FRAP more effectively. It is noticed that synthesized nanozymes have greater antibacterial and anticancer activity but low scavenging ability compared to green extract. Thus, Cu-Mn₃O₄ NPs from Azadirachta indica leaf extract could be utilized as a replacement of potential antibiotic drug candidate against MDR bacteria and in cancer avenues.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"899-912"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.","authors":"Fucheng Zhu, Zixu Yan, Jingli Dai, Juwen Wang, Shiping Huang, Jingbo Ma, Naidong Chen, Yongjun Zang","doi":"10.1007/s00449-025-03153-1","DOIUrl":"10.1007/s00449-025-03153-1","url":null,"abstract":"<p><p>Salidroside has been widely utilized in the food and cosmetics industries. However, the efficient synthesis of salidroside remains a challenge. In this study, a potential uridine diphosphate-dependent glycosyltransferase (UGT) from Bacillus subtilis 168 (named UGT_BS) was identified through evolutionary relationship analysis and molecular docking, with findings subsequently validated by experimental verification. The optimal conversion of UGT_BS for salidroside synthesis reached 98.4% (mol/mol). Additionally, a conversion exceeding 85% (mol/mol) was achieved using the UGT_BS-AtSuSy cascade reaction with tyrosol concentrations ranging from 1 to 10 mM, demonstrating the substrate tolerance of UGT_BS at high concentrations. Kinetic determination and molecular docking confirmed that the strengthened hydrogen bonds and suitable active center conformation between the enzyme and substrate may account for the efficient synthesis of salidroside. Furthermore, 43.5 mM of salidroside was obtained using a fed-batch cascade reaction strategy. The UGT_BS identified in this study shows significant potential for salidroside synthesis, and the strategy employed here serves as a reference for the discovery of related enzymes.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"939-949"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current strategies for rutin nano-formulation; a promising bioactive compound with increased efficacy.","authors":"Maryam Malekpour, Alireza Ebrahiminezhad, Zeinab Karimi, Mahdiyar Iravani Saadi, Aydin Berenjian","doi":"10.1007/s00449-025-03156-y","DOIUrl":"10.1007/s00449-025-03156-y","url":null,"abstract":"<p><p>Rutin is an herbal polyphenolic compound recognized for its numerous therapeutic benefits, including antioxidant, anticancer, and antimicrobial properties. However, its application in biomedical fields encounters significant challenges, such as low solubility, poor absorption, low bioavailability, short half-life, and rapid metabolism. In recent years, advancements in nanotechnology have presented promising solutions to these limitations. Consequently, various nano-formulation strategies have been developed to enhance rutin's solubility, absorption, and overall efficacy. These strategies can be broadly categorized into two approaches. The first involves transforming rutin into nanocrystals without the use of any secondary compounds. The second approach entails nano-formulating rutin with other compounds, including proteins, polysaccharides, lipids, polymers, and metals. This study offers a review of these approaches and their applications in biomedical sciences, focusing on their categories, preparation methods, and biomedical properties. Initially, the pharmacological potential of rutin, its application in recent clinical trials, and its mechanisms of action are outlined. Next, it explores how nano-carriers can enhance rutin's bioavailability. Subsequently, the types of nanostructures employed are categorized, along with a discussion of their fabrication methods and benefits. In some instances, certain drawbacks are also reported. The data provided can guide the selection of optimal strategies for rutin nano-formulation, based on the intended biomedical application.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"877-898"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the impact of polydopamine hydrogel electrodes with various doping methods on the performance of microbial fuel cells.","authors":"Jinhu Ma, Ye Chen, Qing Wen","doi":"10.1007/s00449-025-03154-0","DOIUrl":"10.1007/s00449-025-03154-0","url":null,"abstract":"<p><p>Microbial fuel cells (MFCs) have attracted considerable interest as a promising bioelectrochemical technology for directly converting chemical energy into electrical energy. However, their performance remains limited by the properties of anode materials and their interactions with microbial communities. In this study, PPy-MXene/PDA and PDA-PPy-MXene composite hydrogel electrodes (PMP and PPM) were fabricated on a conductive carbon felt substrate to systematically evaluate the influence of different PDA doping strategies on electrode performance. The PMP electrode exhibited a maximum power density of 3.62 W/m², which represented a 34.6% increase compared to the PPM electrode (2.69 W/m²). Moreover, the protein content on the PMP electrode reached 38.05 ± 4.88 mg/cm², 3.79 times higher than that on the PPM electrode (10.05 ± 3.05 mg/cm²). High-throughput sequencing of the 16S rRNA gene revealed that the relative abundance of Geobacter on the PMP electrode surface reached 73.66%, significantly higher than the 51.17% observed on the PPM electrode. These results are attributed to the PDA doping method involving secondary deposition on the electrode surface. This method optimizes the electron transfer pathways and significantly enhances the electrode's conductivity and electrochemical activity by altering the surface roughness of the electrode and increasing the content of hydrophilic functional groups. Consequently, it significantly promotes the enrichment of electroactive microorganisms and improves the efficiency of extracellular electron transfer. This study optimized PDA doping strategies to significantly enhance the electrochemical performance of MFCs, providing new insights and approaches for the rational design of high-performance bioelectrochemical electrodes.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"951-970"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}