Bioprocess and Biosystems Engineering最新文献

Cold-active lipase from Psychrobacter alimentarius ILMKVIT and its application in selective enrichment of ω-3 polyunsaturated fatty acids in flax seed oil.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-20 DOI: 10.1007/s00449-024-03121-1

V Iswareya Lakshimi, M Kavitha

{"title":"Cold-active lipase from Psychrobacter alimentarius ILMKVIT and its application in selective enrichment of ω-3 polyunsaturated fatty acids in flax seed oil.","authors":"V Iswareya Lakshimi, M Kavitha","doi":"10.1007/s00449-024-03121-1","DOIUrl":"https://doi.org/10.1007/s00449-024-03121-1","url":null,"abstract":"Lipases are one of the ubiquitous enzymes that belong to the hydrolases family and have a wide variety of applications. Cold-active lipases are of major attraction as they can act in lower temperatures and low water conditions because of their inherent greater flexibility. One of the novel applications of lipase is the enrichment of ω-3 polyunsaturated fatty acids (PUFA) in plant and fish oils. This study is aimed at the isolation and identification of cold-active lipase producing bacterium from marine sources, preliminary optimization of medium constituents and conditions, purification of lipase using chromatographic techniques, biochemical characterization, and ultimately the exploration of its application in the enrichment of ω-3 PUFA in flax seed oil. Psychrobacter alimentarius ILMKVIT was identified as the potential cold-active lipase producing bacterium based on its lipolytic activity in rhodamine B agar, titrimetric, and p-nitrophenyl palmitate (p-NPP) assays. One factor at a time (OFAT) analysis, revealed, an incubation time of 4.5 days, alkaline pH of 9, the temperature of 25 °C, peptone, and yeast extract as nitrogen sources, olive oil as inducer sources, 1% inoculum size, and NaCl as mineral sources as optimum production medium constituents and conditions for lipase production. Lipase purification was achieved by ion exchange and gel-filtration chromatography with a 9.27% yield and 37.51-fold purification. Biochemical characterization reported that the lipase is cold-active, alkaline, enhanced by Fe3+ metal ions, and tolerant to organic solvents, detergents, and inhibitors. P. alimentarius ILMKVIT lipase-hydrolysis followed by urea complexation of flax seed oil resulted in the enrichment of ω-3 PUFA, especially α-linolenic acid (ALA). Hence, the novel cold-active lipase from P. alimentarius ILMKVIT could be used to enrich ω-3 PUFA in flax seed oil and developed further as a prominent nutrient supplement for health benefits.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of the phenolic release and conversion in oats (Avena sativa L.) by co-microbiological fermentation with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-20 DOI: 10.1007/s00449-024-03109-x

Zixuan Wang, Du Luo, Wenjing Xu, Haoqun Liu, Mei Pang, Gong Chen

{"title":"Regulation of the phenolic release and conversion in oats (Avena sativa L.) by co-microbiological fermentation with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis.","authors":"Zixuan Wang, Du Luo, Wenjing Xu, Haoqun Liu, Mei Pang, Gong Chen","doi":"10.1007/s00449-024-03109-x","DOIUrl":"https://doi.org/10.1007/s00449-024-03109-x","url":null,"abstract":"Microbial fermentation is an effective method to improve the functional activity of oats (Avena sativa L.), while there are some limitations to the advantages of single microbial fermentation. In this study, a microbial co-culture fermentation system with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis to release and conversion oat phenolics was established. Results showed that the optimal microbial co-fermentation system was obtained by adding Saccharomyces cerevisiae on the fourth day and Bacillus subtilis on the eighth day during Monascus anka fermentation (MF + 4S + 8B). The phenolic content was reached 26.93 mg GAE/g DW, which increased 41.08 times compared to un-fermented oats (UF). In the process of co-fermentation systems, cellulase and β-glucosidase (r2 = 0.97, p < 0.01) had a positive correlation with the release of phenolics. SEM combined with HPLC showed that the complex enzyme system produced by microbial co-fermentation enhanced the disruption of oat cell structure, as well as altered the phenolics fractions and facilitated the conversion of bound phenolics to free phenolics, especially the content of chlorogenic acid and vanillic acid in the free forms was increased 31.42 and 14.15 times, respectively. Additionally, the phenolic contents were increased and the components were changed with the microbial co-fermentation of crude enzyme solution further added, which validated the positive influence of complex enzyme system of MF + 4S + 8B in the phenolic release and transformation of oats. Therefore, this study systematically investigated the phenolic mobilization in oats during the co-fermentation period, which provides a viable option for improving the functional properties of cereal products, as well as the application of microbial cell factories.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamma-aminobutyric acid fermentation and its fermented extracts on α-glucosidase inhibition and anti-obesity effect.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-18 DOI: 10.1007/s00449-024-03119-9

Ji Min Kim, Chae Hun Ra

{"title":"Gamma-aminobutyric acid fermentation and its fermented extracts on α-glucosidase inhibition and anti-obesity effect.","authors":"Ji Min Kim, Chae Hun Ra","doi":"10.1007/s00449-024-03119-9","DOIUrl":"https://doi.org/10.1007/s00449-024-03119-9","url":null,"abstract":"Levilactobacillus brevis KCL010 was fermented in a simple medium containing 8% (w/v) of rice bran extract. We modified the carbon, nitrogen, and initial pH conditions using 10 g/L of sucrose, 10 g/L of yeast extract, and 5.0 of pH, respectively. To minimize the pH increase due to decarboxylation, we fermented 100 mL of modified synthetic medium containing citrate-phosphate buffer (CPB, pH 5.0) of 25-200 mM in 250 mL Erlenmeyer flasks. After 72 h of fermentation with 50 mM CPB, the maximum GABA concentration and conversion efficiency were 3.42 g/L and 22.39%. Furthermore, the potential α-glucosidase inhibitory activity, MTT assay, and oil red O staining were determined by fermented extracts of L. brevis KCL010. At the highest concentration of 500 μg/mL, the α-glucosidase inhibition percentages for non-fermented rice bran (NFRB), rice bran fermented by L. brevis (RBFL), and GABA (analytical standard) extracts were 55.03%, 58.37%, and 59.48%, respectively. All extracts exceeded 80% viability, suggesting that there was no cytotoxic to 3T3-L1 adipocytes. The rice bran fermented by L. brevis (RBFL) extract shows a high inhibition of lipid accumulation by 29.33% compared to those of extracts.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-17 DOI: 10.1007/s00449-024-03120-2

Amel Harbaoui, Nadia Khelifi, Neyssene Aissaoui, Murielle Muzard, Agathe Martinez, Issam Smaali

{"title":"A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress.","authors":"Amel Harbaoui, Nadia Khelifi, Neyssene Aissaoui, Murielle Muzard, Agathe Martinez, Issam Smaali","doi":"10.1007/s00449-024-03120-2","DOIUrl":"https://doi.org/10.1007/s00449-024-03120-2","url":null,"abstract":"Cyanobacterial exopolysaccharides (EPS) remain released by cyanobacteria in the surrounding environment with the main purpose of protection against harmful environmental conditions. Recently, they have received significant attention due to their unique structural characteristics, functional properties, and potential applications across various fields. The current study describes the evaluation of EPS production under salinity stress from Arthrospira maxima. The application of high salinity up to 40 g/L enhanced EPS production, which was collected and purified by alcohol precipitation followed by membrane dialysis and lyophilization. A yield of 60 mg/L was obtained. The Size exclusion chromatography gave for the purified EPS an apparent molecular weight of 2.1 × 105 Da. Monosaccharide composition showed that EPS is a heteropolymer, with mannose, xylose, and glucuronic acid identified as the predominant monosaccharides and derivatives. Nuclear magnetic resonance spectroscopy (13C and 1H) confirmed that EPS is a heteropolysaccharide, entirely in α- anomeric configuration, with glucuronic acid as a main monomer that is probably linked to mannose and xylose via α-glycosidic linkages. Bioactivity assessment of EPS revealed that it exhibits antibacterial activity against several strains, notably, Bacillus subtilis (MIC: 0.6 ± 0.05 mg/mL), Bacillus cereus (MIC: 1 ± 0.01 mg/mL), Escherichia coli (MIC: 0.8 ± 0.01 mg/mL) and Klebsiella pneumonia (MIC: 0.8 ± 0.01 mg/mL). Antioxidant activity was measured using the DPPH radical scavenging assay, yielding an IC₅₀ of 6.83 mg/mL. Besides, EPS was also found to exhibit an interesting emulsifying property with several oil types, indicating its potential as a versatile biopolymer for applications in various industrial sectors.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate. 通过在线监测氧转移率，对可再生原料中微生物碳源偏好进行免采样调查。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-16 DOI: 10.1007/s00449-024-03117-x

Luca Antonia Grebe, Paul Richter, Torben Altenkirch, Marcel Mann, Markus Jan Müller, Jochen Büchs, Jørgen Barsett Magnus

{"title":"Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.","authors":"Luca Antonia Grebe, Paul Richter, Torben Altenkirch, Marcel Mann, Markus Jan Müller, Jochen Büchs, Jørgen Barsett Magnus","doi":"10.1007/s00449-024-03117-x","DOIUrl":"https://doi.org/10.1007/s00449-024-03117-x","url":null,"abstract":"The transition towards sustainable bioprocesses requires renewable feedstocks to reduce dependency on finite resources. While plant-based feedstocks offer significant potential, their complex composition poses new challenges. The microorganisms often exhibit polyauxic growth when presented with multiple carbon sources simultaneously, consuming them in a distinct order according to their carbon source preferences. The traditional investigation of polyauxic growth involves laborious sampling and offline analysis, hindering high-throughput screenings. This study introduces an efficient method for identifying carbon source consumption and their order of metabolization by various microorganisms using the respiration activity monitoring system (RAMOS) in shake flasks. As aerobic carbon metabolization and oxygen consumption are strictly correlated, the characteristic phases of polyauxic growth are visible in the oxygen transfer rate (OTR) and can be assigned to the respective carbon sources. An extended 16-flask RAMOS enables real-time monitoring of microbial respiration on up to seven carbon sources and one reference cultivation simultaneously, thus providing crucial insights into their metabolization without extensive sampling and offline analysis. The method's accuracy was validated against traditional high-performance liquid chromatography (HPLC). Its applicability to both fast-growing Escherichia coli (investigated carbon sources: glucose, arabinose, sorbitol, xylose, and glycerol) and slow-growing Ustilago trichophora (glucose, glycerol, xylose, sorbitol, rhamnose, galacturonic acid, and lactic acid) was demonstrated. Additionally, it was successfully applied to the plant-based second-generation feedstock corn leaf hydrolysate, revealing the bioavailability of the included carbon sources (glucose, sucrose, arabinose, xylose, and galactose) and their order of metabolization by Ustilago maydis.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced phosphorus release from pig manure by co-fermentation with food waste. 通过与厨余垃圾共同发酵，提高猪粪的磷释放量。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-13 DOI: 10.1007/s00449-024-03118-w

Chang Su, Shun Wang, Jizhong Meng, Xinmin Zhan

引用次数: 0

Extension of first principle elemental balancing soft-sensors by nonlinear reaction kinetics for increased robustness in bioprocess monitoring.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-13 DOI: 10.1007/s00449-024-03111-3

Don Fabian Müller, Daniel Wibbing, Julian Kager

{"title":"Extension of first principle elemental balancing soft-sensors by nonlinear reaction kinetics for increased robustness in bioprocess monitoring.","authors":"Don Fabian Müller, Daniel Wibbing, Julian Kager","doi":"10.1007/s00449-024-03111-3","DOIUrl":"https://doi.org/10.1007/s00449-024-03111-3","url":null,"abstract":"A first principle soft-sensor for biomass and substrate estimation in upstream bioprocessing based on the fusion of elemental balancing and nonlinear kinetics is presented. It aims to extend the validity range of well-established elemental balancing soft sensors to substrate saturated and overfeeding conditions that often occur in induced production phases. An experimental study with recombinant E. coli cultivations was conducted to illustrate the soft-sensor principle and to analyze the accuracy as well as generalizability of the approach. Under substrate limited growth the extended soft-sensor showed similar performance as classical elemental balancing. In induced production phases however, a decline in maximum substrate uptake capacity ( <math><msub><mi>q</mi> <mrow><mi>Smax</mi></mrow> </msub> </math> ) of up to 80% was observed, where the extended soft-sensor showed up to 41 % better estimates for the biomass and up to 75 % better estimates for the substrate in terms of NRMSE. The paper discusses the possible benefits as well as the requirements for the implementation of the extended elemental balancing soft-sensor.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upflow blanket filter anammox (UBFA) system treating low-nitrogen wastewater: high-efficient nitrogen removal, granules formation, N₂O emission, and microbial succession.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-09 DOI: 10.1007/s00449-024-03116-y

Chongyang Wang, Feng Gao, Sheng Gao, Zheng Nian, Xintong Han

{"title":"Upflow blanket filter anammox (UBFA) system treating low-nitrogen wastewater: high-efficient nitrogen removal, granules formation, N2O emission, and microbial succession.","authors":"Chongyang Wang, Feng Gao, Sheng Gao, Zheng Nian, Xintong Han","doi":"10.1007/s00449-024-03116-y","DOIUrl":"https://doi.org/10.1007/s00449-024-03116-y","url":null,"abstract":"This research provides an important approach for low-nitrogen wastewater treatment through anaerobic ammonium oxidation (Anammox), and Anammox granule sludge (AnGS) in the Upflow. Blanket Filter Anammox (UBFA) system through shortening the hydraulic retention time was successfully cultivated. The percentage of medium granules (1.0-2.0 mm) with the highest Anammox activity increased from 0 to 28.5%, and the proportion of flocs (0-200 μm) reduced from 84.5% to 17.6%. Through the multidimensional analysis of AnGS, the relationship between AnGS and EPS secretion, low SVI, high PN/PS, multiple filamentous bacteria, and AnAOB were explored. Microelectrode tracing tests demonstrated that the main anammox reaction active layer was 0-1500 μm, and the highest activity was observed at 200-400 μm, whereas denitrification activity and N2O production were mainly distributed in the granules deep layer of 1500-2500 μm. The research showed that Candidatus Brocadia and Candidatus Kuenenia were the predominant anammox species in the UBFA system, while the abundance of AnAOB was higher in medium granules.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asphaltenes biodegradation from heavy crude oils by the yeast Yarrowia lipolytica.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-08 DOI: 10.1007/s00449-024-03114-0

Filipe Smith Buarque, Júlio Cesar Soares Sales, Lívia Cabral Lobo, Erika Christina Ashton Nunes Chrisman, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho

{"title":"Asphaltenes biodegradation from heavy crude oils by the yeast Yarrowia lipolytica.","authors":"Filipe Smith Buarque, Júlio Cesar Soares Sales, Lívia Cabral Lobo, Erika Christina Ashton Nunes Chrisman, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho","doi":"10.1007/s00449-024-03114-0","DOIUrl":"https://doi.org/10.1007/s00449-024-03114-0","url":null,"abstract":"Heavy crude oil reserves are characterized by their high viscosity and density, largely due to significant quantities of asphaltenes. The removal of asphaltene precipitates from oil industry installations is crucial, as they can contaminate catalysts and obstruct pipelines. Therefore, this study aimed to bio-transform heavy oil asphaltenes into smaller molecules using the yeast Yarrowia lipolytica, known for its ability to efficiently degrade hydrophobic substrates. For this purpose, asphaltenes were extracted from crude oil samples, and yeast growth was assessed in a mineral medium containing 2, 5, or 10 g L-1 of asphaltenes. After 168 h of incubation, liquid-liquid extraction was conducted on samples from the Yarrowia lipolytica growth medium using chloroform. The extracted fractions were then quantified by gas chromatography. The results indicated that the yeast could utilize the asphaltenes as a carbon source for growth, though there was a delay in growth compared to the control (glucose as the carbon source), with a maximum biomass concentration of 2.26 g L-1 achieved at 144 h. From the experimental design, it was determined that a higher concentration of aromatic compounds was achieved under the conditions of 115 rpm, 2 g L-1 of asphaltenes, and 0.5 g L-1 of cell inoculum. Conversely, to obtain a higher concentration of saturated compounds, the optimal conditions were 160 rpm, 5 g L-1 of asphaltenes, and 1.0 g L-1 of cell inoculum. Molecular docking results indicated that asphaltenes have a high affinity for cytochrome P450, laccase, and Lip2, with interactions observed with their catalytic triads, suggesting a significant role for these enzymes in asphaltene bioconversion.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-content imaging and deep learning-driven detection of infectious bacteria in wounds.

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2024-12-02 DOI: 10.1007/s00449-024-03110-4

Ziyi Zhang, Lanmei Gao, Houbing Zheng, Yi Zhong, Gaozheng Li, Zhaoting Ye, Qi Sun, Biao Wang, Zuquan Weng

{"title":"High-content imaging and deep learning-driven detection of infectious bacteria in wounds.","authors":"Ziyi Zhang, Lanmei Gao, Houbing Zheng, Yi Zhong, Gaozheng Li, Zhaoting Ye, Qi Sun, Biao Wang, Zuquan Weng","doi":"10.1007/s00449-024-03110-4","DOIUrl":"https://doi.org/10.1007/s00449-024-03110-4","url":null,"abstract":"Fast and accurate detection of infectious bacteria in wounds is crucial for effective clinical treatment. However, traditional methods take over 24 h to yield results, which is inadequate for urgent clinical needs. Here, we introduce a deep learning-driven framework that detects and classifies four bacteria commonly found in wounds: Acinetobacter baumannii (AB), Escherichia coli (EC), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). This framework leverages the pretrained ResNet50 deep learning architecture, trained on manually collected periodic bacterial colony-growth images from high-content imaging. In in vitro samples, our method achieves a detection rate of over 95% for early colonies cultured for 8 h, reducing detection time by more than 12 h compared to traditional Environmental Protection Agency (EPA)-approved methods. For colony classification, it identifies AB, EC, PA, and SA colonies with accuracies of 96%, 97%, 96%, and 98%, respectively. For mixed bacterial samples, it identifies colonies with 95% accuracy and classifies them with 93% precision. In mouse wound samples, the method identifies over 90% of developing bacterial colonies and classifies colony types with an average accuracy of over 94%. These results highlight the framework's potential for improving the clinical treatment of wound infections. Besides, the framework provides the detection results with key feature visualization, which enhance the prediction credibility for users. To summarize, the proposed framework enables high-throughput identification, significantly reducing detection time and providing a cost-effective tool for early bacterial detection.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0