Bioprocess and Biosystems Engineering最新文献

Sustainable synthesis of alginate-cobalt ferrite nanocomposites for horseradish peroxidase immobilization: enhanced stability, reusability, and catalytic efficiency. 用于辣根过氧化物酶固定化的海藻酸盐-钴铁氧体纳米复合材料的可持续合成：增强稳定性、可重复使用性和催化效率。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-23 DOI: 10.1007/s00449-025-03171-z

Abdullah N Alotaibi, Abdullah Al-Dakhil, Hayam A Alwabsi, Ibrahim O Althobaiti, Reda M El-Shishtawy, Yaaser Q Almulaiky

{"title":"Sustainable synthesis of alginate-cobalt ferrite nanocomposites for horseradish peroxidase immobilization: enhanced stability, reusability, and catalytic efficiency.","authors":"Abdullah N Alotaibi, Abdullah Al-Dakhil, Hayam A Alwabsi, Ibrahim O Althobaiti, Reda M El-Shishtawy, Yaaser Q Almulaiky","doi":"10.1007/s00449-025-03171-z","DOIUrl":"10.1007/s00449-025-03171-z","url":null,"abstract":"This study explores the green synthesis of an alginate-cobalt ferrite (Alg-CoFe2O4) composite using Brachychiton populneus extract for horseradish peroxidase (HRP) immobilization, targeting enhanced enzyme stability and reusability. The Alg-CoFe2O4 composite, synthesized via co-precipitation and calcination at 600 °C, was characterized using XRD, VSM, SEM-EDX, BET, FT-IR, and zeta potential analysis, confirming its structural, magnetic, and surface properties. HRP immobilization via glutaraldehyde crosslinking achieved a high immobilization yield of 84%, attributed to the composite's high surface area and covalent binding efficiency. Kinetic analysis revealed an increased Km (30 mM) and Vmax (5.88 µmol/min) for HRP@Alg-CoFe2O4, indicating enhanced catalytic efficiency with reduced substrate affinity. The immobilized enzyme exhibited superior tolerance to organic solvents, retaining 149% activity in n-hexane. In addition, it retained 71% activity after 10 cycles and 74% after 8 weeks of storage at 4 °C, outperforming free HRP. Optimal conditions shifted from pH 6.5 to 7.0 and from 50 to 60 °C, reflecting improved environmental resilience. This study highlights HRP@Alg-CoFe2O4 as a robust biocatalyst, offering promising applications in wastewater treatment and industrial bioprocesses.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1207-1219"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual roles of anion exchange resin in green lignocellulose biorefinery system based on self-providing acid pretreatment. 阴离子交换树脂在自备酸预处理绿色木质纤维素生物炼制体系中的双重作用。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-17 DOI: 10.1007/s00449-025-03166-w

Yang Lv, Haijiong Lu, Alfred Elikem Kwami Afedzi, Ikram Ul Haq, Yong Xu

引用次数: 0

Aegle marmelos-mediated zinc oxide nanoparticles for mosquito control, cancer therapy, and antibacterial applications. 凝胶介导的氧化锌纳米颗粒用于蚊虫控制，癌症治疗和抗菌应用。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-28 DOI: 10.1007/s00449-025-03162-0

Nandhini Vasu, Thangamathi Perumal, Prabu Kumar Seetharaman, Ananth Sivapunniyam, Gnanasoundari Anthoni, Karthik Raja Ramalingam, Vahitha Rajamohan, Sharmiladevi Thangaiyan

{"title":"Aegle marmelos-mediated zinc oxide nanoparticles for mosquito control, cancer therapy, and antibacterial applications.","authors":"Nandhini Vasu, Thangamathi Perumal, Prabu Kumar Seetharaman, Ananth Sivapunniyam, Gnanasoundari Anthoni, Karthik Raja Ramalingam, Vahitha Rajamohan, Sharmiladevi Thangaiyan","doi":"10.1007/s00449-025-03162-0","DOIUrl":"10.1007/s00449-025-03162-0","url":null,"abstract":"The study investigated the synthesis and characterization of zinc oxide nanoparticles (Am-ZnO-NPs) using Aegle marmelos leaves and their mosquitocidal, antioxidant, and anticancer efficacy. Am-ZnO-NPs were synthesized via combustion method under optimized conditions, including 5% plant extract, temperature of 70 °C, a contact time of 60 min, and pH of 10. The resulting nanoparticles were characterized using advanced analytical techniques. The Am-CuO-NPs were hexagonal wurtzite in shape and had an average particle size of 37.44 nm. The Am-CuO-NPs are highly stable with a surface charge of - 26.77 mV. Gas chromatography-mass spectrometry (GC-MS) analysis identified several compounds with insecticidal, antioxidant, and anticancer properties, including hexadecenoic acid, ethyl ester (25.61%), 9,12-octadecadienoic acid, ethyl ester (12.64%), and 9,12,15-octadecatrienoic acid, ethyl ester (6.31%). The aqueous extract of A. marmelos (100 µg/mL) demonstrated the highest larvicidal (57.33%) and pupicidal (42.67%) activities compared to other solvent extracts. In contrast, Am-ZnO-NPs exhibited relatively higher larvicidal activity (86.67%) and pupicidal (56.00%) at 10 µg/mL. The nanoparticles also inhibited the activities of acetylcholinesterase (AChE) and carboxylesterases and reduced the total protein (TP) levels in Ae. aegypti larvae. Further, both the aqueous leaf extract and Am-ZnO-NPs were effective in scavenging DPPH free radicals, achieving 76% and 94% inhibition, respectively, at a concentration of 125 µg/mL. Am-ZnO-NPs also exhibited cytotoxicity, induced cell cycle arrest, enhanced cell adhesion, and promoted apoptosis in L-132 lung cancer cell lines. Further, the Am-ZnO-NPs display potent antibacterial activity against clinical pathogens. These results highlight the role of phytochemicals in A. marmelos leaves for their effective biological activities.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1089-1111"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plants lipases: challenges, recent advances, and future prospects - a review. 植物脂肪酶：挑战、最新进展及未来展望

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-12 DOI: 10.1007/s00449-025-03164-y

Nicole Novelli do Nascimento, Ana Bárbara Moulin Cansian, Jumara Silva de Sousa, Fernanda Novelli Negrão, Paulo Waldir Tardioli, Angélica Marquetotti Salcedo Vieira

{"title":"Plants lipases: challenges, recent advances, and future prospects - a review.","authors":"Nicole Novelli do Nascimento, Ana Bárbara Moulin Cansian, Jumara Silva de Sousa, Fernanda Novelli Negrão, Paulo Waldir Tardioli, Angélica Marquetotti Salcedo Vieira","doi":"10.1007/s00449-025-03164-y","DOIUrl":"10.1007/s00449-025-03164-y","url":null,"abstract":"Plant lipases offer a sustainable and promising alternative for various industrial applications, with increasing use in biocatalytic processes in recent years. Leveraging plants as renewable resources reduces dependence on animal or microbial sources, providing significant potential for sustainable lipase production. These lipases are biodegradable and less toxic, enhancing their cost-effectiveness, particularly when sourced from plants with additional economic value. The diversity of plant species offers a wide array of lipases with different properties, broadening their industrial applications. Additionally, integrating plant lipase production into existing agricultural processes by using agricultural residues or by-products as enzyme sources can reduce costs and add value to waste materials. Despite their potential, several challenges must be addressed for the effective utilization of plant-derived lipases. Reducing extraction and purification costs is essential to make these enzymes competitive with other sources. Advancements in the biochemical and structural characterization of plant lipases have facilitated enzymatic engineering approaches to enhance enzyme stability, specificity, and catalytic efficiency. A review of the current research can help identify gaps and suggest new directions for enzyme development and technological advancements. Understanding the mechanisms of action and unique properties of plant lipases can drive innovations in biocatalytic processes. This review aims to highlight the characteristics of plant lipases and the challenges in their extraction, purification, and stability. This study conducted a narrative review using a database of relevant studies, selecting 92 studies. The future of plant lipases holds great promise for transformative impacts across various industries, promoting more sustainable and innovative practices.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1049-1067"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient pretreatment of Phragmites australis biomass using glutamic acid for bioethanol production by a hybrid hydrolysis and fermentation strategy. 谷氨酸水解与发酵混合预处理芦苇生物质制备生物乙醇的研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-13 DOI: 10.1007/s00449-025-03165-x

Jiabin Wang, Rui Zhang, Yu Shao, Cheng Zhang, Xinyan You, Qianyue Yang, Fang Xie, Rongling Yang, Hongzhen Luo

{"title":"Efficient pretreatment of Phragmites australis biomass using glutamic acid for bioethanol production by a hybrid hydrolysis and fermentation strategy.","authors":"Jiabin Wang, Rui Zhang, Yu Shao, Cheng Zhang, Xinyan You, Qianyue Yang, Fang Xie, Rongling Yang, Hongzhen Luo","doi":"10.1007/s00449-025-03165-x","DOIUrl":"10.1007/s00449-025-03165-x","url":null,"abstract":"Microbial fermentation of renewable lignocellulosic biomass to produce biofuels presents significant environmental advantages. The conversion of cellulose and hemicellulose into fermentable sugars provides essential carbon sources for microbial metabolism. However, the recalcitrance of biomass limits enzymatic accessibility. In this study, mild L-glutamic acid (GA) pretreatment was applied to Phragmites australis residues (reed straw) to fractionate lignin and polysaccharides for enhancing enzymatic hydrolysis. Pretreatment with 0.20 mol/L GA at 180 °C for 50 min (logRo = 4.1) achieved glucan recovery and xylan removal rates of 84.2% and 87.8%. Consequently, glucose and total sugar yields reached 75.5 and 71.2%, representing 5.35- and 5.18-fold increases compared to untreated reed. The 28.7 g fermentable sugars with a high glucose-to-xylose ratio (18.1 g/g) were obtained from 100 g reed. The hydrolysates were subsequently used as substrates for bioethanol production by Saccharomyces cerevisiae, which yielded 12.4-32.3 g/L ethanol via separate hydrolysis and fermentation (SHF). By analyzing bioethanol production of SHF and simultaneous saccharification and fermentation (SSF), an optimized hybrid hydrolysis and fermentation (HHF) strategy was developed. Under HHF process, 48.5 g/L of ethanol was achieved from 20 wt% solid loads. This study demonstrates an efficient approach to convert abundant lignocellulosic waste into fermentable sugars and biofuels.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1133-1146"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clay mineral content modulates biogenic gas production in coal: divergent microbial responses in low- and medium-rank coals revealed by multi-omics. 黏土矿物含量调节煤中生物气的产生：多组学揭示了中低煤中不同的微生物反应。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-22 DOI: 10.1007/s00449-025-03170-0

Shufeng Zhao, Hongyu Guo, Zebin Wang, Bin Zhang, Hao Chen, Norbert Klitzsch, Lijiao Yue, Daping Xia

{"title":"Clay mineral content modulates biogenic gas production in coal: divergent microbial responses in low- and medium-rank coals revealed by multi-omics.","authors":"Shufeng Zhao, Hongyu Guo, Zebin Wang, Bin Zhang, Hao Chen, Norbert Klitzsch, Lijiao Yue, Daping Xia","doi":"10.1007/s00449-025-03170-0","DOIUrl":"10.1007/s00449-025-03170-0","url":null,"abstract":"The influence of clay mineral content on biogenic gas production in coal seams remains insufficiently understood. This study systematically investigated the mechanisms by which clay minerals affect biogas production in low- and medium-rank coals by integrating simulated biogas production experiments with multidimensional analytical techniques, including infrared spectroscopy, X-ray diffraction, scanning electron microscopy, gas chromatography-mass spectrometry, fluorescence spectroscopy, and metagenomic analysis. The results demonstrated that in low-rank coal, increasing the clay content from 2.78 to 4.75 g per 20 g of coal reduced the biogas yield from 6.30 to 3.47 mL/g. Conversely, in medium-rank coal, increasing the clay content from 1.66 to 2.65 g per 20 g of coal enhanced the biogas yield from 3.45 to 5.28 mL/g. These contrasting outcomes are primarily attributed to the distinct mechanistic roles of clay minerals across coal ranks. In low-rank coal, the hydration-induced swelling of clay minerals intensified pore blockage, impeded gas diffusion, decreased the abundance of genes involved in propionate degradation, and suppressed microbial metabolic activity, ultimately limiting methane production. In contrast, in medium-rank coal, clay minerals facilitated the enrichment of key functional microbial taxa, such as Acetobacteroides and Methanoculleus, promoted the degradation of fatty acids, hydroxyls, and amines, and enhanced the activity of acidogenic and methanogenic pathways, thereby increasing methane yield. This study elucidates the microbial mechanisms underlying the regulatory role of clay minerals in biogas production, offering new theoretical insights into the origin of coalbed methane (CBM) and providing a scientific foundation for optimizing biogenic CBM recovery.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1189-1205"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of paddlewheel geometry on hydrodynamic performance and energy consumption in microalgae cultivations in open raceway ponds. 桨轮几何形状对开放式滚道池微藻养殖水动力性能和能量消耗的影响

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-23 DOI: 10.1007/s00449-025-03168-8

Gabriel de Morais Campos, Natalia Dos Santos Renato, Victor Hugo Laviola de Oliveira, Pedro Henrique de Moura Rodrigues, Marcio Arêdes Martins

{"title":"Influence of paddlewheel geometry on hydrodynamic performance and energy consumption in microalgae cultivations in open raceway ponds.","authors":"Gabriel de Morais Campos, Natalia Dos Santos Renato, Victor Hugo Laviola de Oliveira, Pedro Henrique de Moura Rodrigues, Marcio Arêdes Martins","doi":"10.1007/s00449-025-03168-8","DOIUrl":"10.1007/s00449-025-03168-8","url":null,"abstract":"Microalgae cultivation is a field with significant potential across various industries, such as pharmaceuticals, food, cosmetics, and energy. Aiming to optimize the geometric parameters of a paddlewheel agitation system through computational fluid dynamics (CFD) and experimental validation, an investigation was conducted involving different rotation speeds, blade pitch angles, and the number of blades on a paddlewheel within a microalgae cultivation tank. The results revealed paddlewheels with 90° inclined blades exhibited higher average flow velocities. Regarding the vertical mixing index, the 8-blade paddlewheels demonstrated inferior performance compared to systems with four and six blades. A pitch angle of 60° minimized power consumption. The optimal configuration found was a 60°-angled 4-blade paddlewheel, operating at a rotation speed of 19 rpm, yielding the highest mixing performance index value at 46.12 W-1. The straight blades operated at 13 rpm could not sustain microalgae suspension. For 60° inclined blades, all tested rotations achieved a high level of suspension.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1171-1188"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved productivity of recombinant adeno-associated virus (rAAV) via triple transfection of HEK293 cells using perfusion cultivation. 通过灌注培养三次转染HEK293细胞，提高重组腺相关病毒（rAAV）的产率。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-26 DOI: 10.1007/s00449-025-03167-9

Jianqi Nie, Zhaojing Xu, Yang Sun, He Ren, Zichuan Song, Yan Zhang, Zhonghu Bai

{"title":"Improved productivity of recombinant adeno-associated virus (rAAV) via triple transfection of HEK293 cells using perfusion cultivation.","authors":"Jianqi Nie, Zhaojing Xu, Yang Sun, He Ren, Zichuan Song, Yan Zhang, Zhonghu Bai","doi":"10.1007/s00449-025-03167-9","DOIUrl":"10.1007/s00449-025-03167-9","url":null,"abstract":"In recent years, recombinant adeno-associated virus (rAAV) vectors are the promising viral transfer tools for gene therapy and clinical trials, thanks to their favorable safety profile and long-term transgene expression. The increasing demand for rAAVs for gene therapy led to a rise in the amount of these vectors required for pre-clinical trials, clinical trials, and approved therapeutic applications. A majority of suspension HEK293 cell-based rAAV production protocols reported rely on a triple transfection at cell density below 2 × 106 cells/mL. However, the low yield of such biomanufacturing challenges bioprocess engineers to develop more efficient strategies capable of increasing volumetric productivity. In this study, we developed a perfusion bioprocess to enable rAAV production efficiently at high cell density. We first optimized three key process parameters (the total DNA amount, ratio of polyethyleneimine (PEI) to DNA, and proportion of the three plasmids) of rAAV production at cell density of 2 × 106 cells/mL by the design of experiment method, from which the robust setpoint (total DNA amount of 1.37 μg/mL, ratio of PEI to DNA of 1.52 μL/μg, the proportion of plasmids pHelper 24%, pRC 46%, pGOI 30%) was explored. We then developed a rAAV production process at a cell density of ~ 8 × 106 cells/mL, with increasing DNA amount on a cell basis and optimizing transfection complex preparation. This approach was confirmed in a 5 L benchtop bioreactor connected with a perfusion system, resulting in a viral genomic titer of 7.28 × 1011 vg/mL and a cell-specific viral genomic titer of 4.97 × 104 vg/cell. This study demonstrates that the perfusion process coupled with optimized transfection complex preparation has the potential to improve manufacturing productivity.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1159-1169"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of saline stress in alternating pulses on aerobic granulation and resource production using different inoculum sources. 交替脉冲盐胁迫对不同接种源的好氧肉芽和资源生产的影响。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-13 DOI: 10.1007/s00449-025-03163-z

Clara Bandeira de Carvalho, Vicente Elício Porfiro Sales Gonçalves da Silva, Francisca Kamila Amancio Frutuoso, André Bezerra Dos Santos

{"title":"Influence of saline stress in alternating pulses on aerobic granulation and resource production using different inoculum sources.","authors":"Clara Bandeira de Carvalho, Vicente Elício Porfiro Sales Gonçalves da Silva, Francisca Kamila Amancio Frutuoso, André Bezerra Dos Santos","doi":"10.1007/s00449-025-03163-z","DOIUrl":"10.1007/s00449-025-03163-z","url":null,"abstract":"Aerobic granular sludge (AGS) is a promising technology for wastewater treatment, particularly for its ability to recover valuable resources such as polyhydroxyalkanoates, alginate-like exopolysaccharide, and phosphorus. However, achieving stable granule formation remains a significant challenge. Research has shown that the addition of salt can accelerate the granulation process and enhance bioresource production. The source of the seed biomass is also critical for the system's success, with most AGS studies using activated sludge as the inoculum. This study aims to compare granulation, reactor performance, and bioresource recovery outcomes using inocula from different sources while also evaluating the impact of saline stress. Four sequential batch reactors were monitored, differing in the type of inoculum sludge (biomass from an aerated biofilter or activated sludge systems) and the presence of NaCl in the feed. The saline feed alternated between cycles containing 5 gNaCl/L and conventional feed without NaCl. Osmotic pressure was found to favor granulation and solids accumulation in both types of biomasses. Reactors inoculated with activated sludge and subjected to salt addition achieved complete granulation more rapidly. In contrast, reactors inoculated with submerged aerated biofilter sludge exhibited higher solids concentrations. All systems demonstrated excellent chemical oxygen demand removal, with activated sludge reactors showing superior performance in ammonia and total nitrogen removal and bioresources recovery. Salt addition stimulated the production of extracellular polymeric substances and amino acids such as tyrosine and tryptophan while reducing the intensity of fulvic acid-like substances, irrespective of the inoculum type.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1113-1132"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Itaconic acid production from corn stover hydrolysates for a newly isolated Aspergillus terreus through adaptive evolution. 一种新分离的土曲霉通过适应进化从玉米秸秆水解物中生产衣康酸。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-14 DOI: 10.1007/s00449-025-03161-1

Xia Yi, Xinji Li, Jianqi Han, Zhidan Liu, Xiaohui Shi, Tao Wen, Jie Zhu

{"title":"Itaconic acid production from corn stover hydrolysates for a newly isolated Aspergillus terreus through adaptive evolution.","authors":"Xia Yi, Xinji Li, Jianqi Han, Zhidan Liu, Xiaohui Shi, Tao Wen, Jie Zhu","doi":"10.1007/s00449-025-03161-1","DOIUrl":"10.1007/s00449-025-03161-1","url":null,"abstract":"Itaconic acid can be produced using lignocellulosic biomass; however, the inhibitors from pretreatment process of biorefinery are toxic to the fermenting strains. Here, with 35.70 ± 0.69 g/L (0.19 ± 0.05 g/L·h and 73.84 ± 0.01%) itaconic acid from shake flask fermentation of synthetic medium (SM), a newly isolated Aspergillus terreus just produced 1.01 ± 0.01 g/L itaconic acid from corn stover hydrolysates (CSH) for the serious block of aldehyde inhibitors and acetic acid. Convincingly, 25.34 ± 3.94 g/L (0.13 ± 0.02 g/L·h and 37.92 ± 3.89%) itaconic acid was achieved from the detoxified CSH (with residual 0.49 g/L acetic acid) using 4.0% activated charcoal. 21.64 ± 2.42 g/L (0.05 ± 0.01 g/L·h and 26.96 ± 7.81%) itaconic acid was further achieved from CSH for the adapted A. terreus with better degradation ability of furanic aldehydes and phenolic aldehydes. Furthermore, the 108 mutation sites of nine genes from adaptive laboratory evolution (ALE) for A. terreus were further uncovered through single nucleotide polymorphisms (SNPs) analysis, and thus would be responsible for the improved fermentability of itaconic acid from CSH. The current work broke the bottlenecks in itaconic acid fermentation directly from CSH through improving A. terreus using directed evolution technique, and thus would provide a strain biocatalyst A. terreus and establish the alternative strategy to efficiently produce itaconic acid using corn stover.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1069-1087"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0