Bioprocess and Biosystems Engineering最新文献

{"title":"Investigation of antioxidant, antibacterial, anticancer and wound healing properties of eco-friendly synthesized copper oxide nanoparticles from Plumeria rubra leaf extract.","authors":"Annapoorani Angusamy, Subramanian Palanisamy, Sangeetha Ravi, Manikandan Kumaresan, Sonaimuthu Mohandoss, SangGuan You, Manikandan Ramar","doi":"10.1007/s00449-025-03176-8","DOIUrl":"10.1007/s00449-025-03176-8","url":null,"abstract":"<p><p>Plants serve as a rich source of bioactive agents and coupling them with carriers using nanotechnology has recently become an effective therapeutic approach in pharmacognosy. Metal oxides, especially copper oxide (CuO), have been employed in synthesizing nanoparticles due to their efficient reducing properties. The purpose of this work was to examine the physicochemical, antioxidant, antibacterial, anticancer, and wound healing abilities of copper oxide nanoparticles (CuONPs) synthesized using Plumeria rubra leaf extract. FTIR, XRD, FESEM, EDX, AFM, and UV-vis spectroscopy were used to confirm the formation of CuONPs, and the results showed that they were spherical in shape and 35 nm in size. DPPH and nitric oxide antioxidant assays revealed that they possess effective free radical scavenging ability. CuONPs showed bactericidal activity against human pathogenic bacteria. The anticancer effect of CuONPs was assessed on the Neuro-2a (N2a) neuroblastoma cells. Both P. rubra leaf extract and CuONPs exhibited dose-dependent cytotoxicity with morphological distortions and apoptosis, along with a loss of membrane integrity. In vivo analysis of CuONPs for their wound healing ability in Wistar albino rats showed a better wound closure percentage compared to that of the control animals. Based on our findings, CuONPs may be applied as a potential therapeutic agent in developing treatments for a spectrum of various diseases.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1295-1310"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nano valent iron size on two-phase anaerobic digestion of food and kitchen waste.","authors":"Xinqiang Ning, Jiahan Huang, Jialun Hu, LuLing Lan, Yuanming Huang, Wei Ding, Tang Tang, Huibo Luo, Zhihui Bai, Hao Chen, Xiaopeng Ge, Lei Li","doi":"10.1007/s00449-025-03184-8","DOIUrl":"10.1007/s00449-025-03184-8","url":null,"abstract":"<p><p>The addition of zero-valent iron (ZVI) to the anaerobic digestion of food and kitchen waste (FKW) can significantly improve methane production efficiency. However, the impact of nano ZVI (nZVI) addition during both acidification and methanogenic phases of the two-phase anaerobic digestion of FKW remains unclear. This study investigated the effect of different nZVI particle sizes (50, 100, and 300 nm) introduced during the acidification phase on the overall performance of two-phase anaerobic digestion. The results revealed that nZVI improved the performance of the acidification phase. Particularly, 50 nm nZVI increased protein concentrations, likely owing to its toxicity, which caused microbial cell damage. The addition of 300 nm nZVI led to higher concentrations of soluble chemical oxygen demand (SCOD) and total volatile fatty acids (TVFAs), reaching 40,302.45 and 10,375.00 mg/L, respectively. In the methanogenic phase, 300 nm nZVI achieved the highest methane production, reaching 799.78 mL/g VS, which was enhanced by the optimal concentrations of TVFAs and Fe²⁺. Moreover, the addition of 300 nm nZVI enriched Bifidobacterium (32.74%) and Clostridium sensu stricto 1 (37.57%), both of which promoted TVFA generation, increased Methanobacterium abundance, and facilitated rapid methane production. Furthermore, 300 nm nZVI enhanced key metabolic pathways, such as transport, catabolism, and amino acid metabolism, thereby increasing methane production in the anaerobic digestion system.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1385-1398"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-optimized bioprocess for macroidin production by Lysinibacillus macroides and its biomedical applications.","authors":"Maurice George Ekpenyong, Philomena Effiom Edet, Atim David Asitok, Andrew Nosakhare Amenaghawon, Stanley Aimhanesi Eshiemogie, David Sam Ubi, Cecilia Uke Echa, Heri Septya Kusuma, Sylvester Peter Antai","doi":"10.1007/s00449-025-03183-9","DOIUrl":"10.1007/s00449-025-03183-9","url":null,"abstract":"<p><p>The quest for solutions to infectious diseases and life-debilitating disease states has been ongoing for centuries now. Natural products researches have revealed bioactive compounds of plant and microbial origin that offer solutions to health conditions but with poor yield. This study reports yield improvement of a novel macroidin bacteriocin through robust comparative process optimization involving statistical and machine learning approaches. Response surface methodology (RSM), artificial neural network (ANN), and extreme gradient boosting (XGBoost) models showed adequate fitting capabilities considering statistical indices and performance errors as: RSM (R² = 0.9389; MSE = 0.3877), ANN (R² = 0.9727; MSE = 0.1379) and XGBoost (R² = 0.8758; MSE = 0.6272). The ANN model, with superior prediction results, was further optimized by evolutionary (genetic algorithm-GA) and swarm (particle swarm optimization) intelligence techniques which increased macroidin concentration by 2.38-fold and 2.2-fold, respectively. ANN's superior parameter generalization and remarkable validation accuracy by GA at 23.1 °C, pH 8.89, 0.5 vvm aeration, and 248.6 rpm agitation selected the ANN-GA model for bioreactor production. The scale-up study revealed a volumetric oxygen transfer coefficient of 33.95 h^-1 at 250 rpm and 0.5 vvm, at which a macroidin yield, Y_p/x of 0.93 g g^-1 and productivity of 2.00 g L^-1 h^-1 were achieved. Evaluated pharmaco-clinical potentials of macroidin revealed significant (p < 0.05) anti-proliferative effects against HepG2 and MCF-7 cell lines and bactericidal and antibiofilm activities against ESKAPE pathogens. The bactericidal action was revealed to proceed through membrane permeability, electrolyte, and ATP depletion, to cell lysis.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1363-1384"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advance on the production of microbial exopolysaccharide from waste materials.","authors":"Zichao Wang, Yi Zheng, Jinghan Guo, Ziru Lai, Jiale Liu, Na Li, Zhitao Li, Minjie Gao, Xueyi Qiao, Yahui Yang, Huiru Zhang, Lemei An, Keyu Xu","doi":"10.1007/s00449-025-03169-7","DOIUrl":"10.1007/s00449-025-03169-7","url":null,"abstract":"<p><p>Polysaccharide has been widely used in the fields of industry, agriculture, food and medicine because of its excellent physicochemical properties and bioactivities. Compared to plant and animal polysaccharides, microbial exopolysaccharide has advantages of occupying less cultivated land, short fermentation period, controllable fermentation process and not restricted by seasons. However, due to the deterioration of global climates and outbreak of conflicts, food crisis has become more and more serious. Therefore, searching alternative substrates for microbial exopolysaccharide production has attracted worldwide attention, waste materials might be an ideal substitute due to its high-content nutrients. Present work discussed and reviewed the production of microbial exopolysaccharide from molasses, cheese whey, lignocellulosic biomass, fruit pomace and/or husk, crude glycerol and kitchen waste. It was found that commercial grade exopolysaccharides were mainly produced from waste materials via submerged fermentation, and pretreatment of waste materials is a commonly used strategy. Although industrial production of microbial exopolysaccharides with waste materials as substrate has not been reported, we hoped that this work could not only provide contribution for efficient utilization of waste materials, but also help for alleviating global food crises.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1239-1254"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of polyethylene glycol and glutaraldehyde as enhancers for lipase-immobilized hybrid organic-inorganic nanoflowers.","authors":"Jessica Siew Kiong Ling, Sie Yon Lau, Shamini Anboo, Murat Yılmaz","doi":"10.1007/s00449-025-03181-x","DOIUrl":"10.1007/s00449-025-03181-x","url":null,"abstract":"<p><p>The present study investigates the influence of polyethylene glycol (PEG) and glutaraldehyde (GA) on the synthesis and enzymatic activity of lipase hybrid nanoflowers. The effect of lipase concentration on hybrid nanoflower formation was first assessed, revealing that the optimum lipase concentration was 0.2 mg/mL. At this concentration, the encapsulation of lipase within the hybrid nanoflowers reached its maximum efficiency. Further, the effects of PEG and GA concentrations, as well as pH, on the enzymatic activity of the nanoflowers were evaluated. The results demonstrated that 2% (v/v) PEG and 3% (v/v) GA were the most effective concentrations, with the highest activity observed at pH 8. Comparative studies showed that GA-treated lipase hybrid nanoflowers exhibited a remarkable 160% increase in enzymatic activity over the free lipase, outperforming PEG in terms of catalytic performance. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) spectroscopy analyses confirmed that both PEG and GA treatments altered the morphology and structural characteristics of the hybrid nanoflowers, with GA inducing more pronounced changes. Despite these morphological alterations, the enzymatic activity was significantly enhanced, particularly in the GA-treated hybrid nanoflowers. In conclusion, this study highlights the superior performance of glutaraldehyde as an enhancer for the production of highly active lipase hybrid nanoflowers, offering promising applications in biocatalysis and enzyme immobilization.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1349-1362"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes and biochemical degradation during dark anoxic incubation of Nannochloropsis: implications for low-energy microalgal cell rupture.","authors":"Bhagya Yatipanthalawa, Esther Mienis, Ronald Halim, Imogen Foubert, Muthupandian Ashokkumar, Peter J Scales, Gregory J O Martin","doi":"10.1007/s00449-025-03185-7","DOIUrl":"10.1007/s00449-025-03185-7","url":null,"abstract":"<p><p>Dark anoxic incubation has been identified as a low-cost method to facilitate the mechanical rupture of microalgae such as Nannochloropsis via autolysis-induced cell wall thinning. During this process, concentrated slurries of cells are incubated in the dark at an elevated temperature, to deprive them of light and oxygen. This work analyzed the integrity of proteins and lipids during dark anoxic incubation and investigated the cellular responses of Nannochloropsis through an in-depth proteomic analysis. Proteomic analysis identified enzymes associated with cellulose hydrolysis and glycolytic and fermentative pathways that are presumably activated to produce energy in the absence of light and oxygen. Progressive biochemical degradation was observed during 48 h of incubation, including the proteolysis and leakage of proteins, and the lipolysis and subsequent peroxidation of lipids. This provides further evidence of autolytic processes occurring during prolonged incubation, which can be attributed to uncontrolled action of intracellular proteases and lipases. Importantly, the resultant formation of peptides and free fatty acids will affect their use in food and fuel applications. It is therefore important to optimise the incubation time and parameters to achieve cell weakening while minimising the unnecessary degradation of biomacromolecules.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1399-1420"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the biochemical and kinetic properties of Candida antarctica lipase immobilized on magnetized poly(styrene-co-ethylene glycol dimethacrylate) and the development of a mathematical model for emollient ester synthesis.","authors":"Gabrielle P Assis, Amanda B S Rangel, Vinicius S Sampaio, Gian F C Anjos, Mateus V C Silva, Leandro G Aguiar, Larissa Freitas","doi":"10.1007/s00449-025-03174-w","DOIUrl":"10.1007/s00449-025-03174-w","url":null,"abstract":"<p><p>The present study aimed to develop a biocatalyst through the immobilization of Candida antarctica lipase B (CALB) via physical adsorption onto magnetized poly(styrene-co-ethylene glycol dimethacrylate) (STY-EGDMA-M). Biochemical property characterization, apparent kinetic parameter determination, and thermal stability assessment were conducted using a methodology developed based on the hydrolysis of the ester methyl butyrate. The results demonstrated that immobilization expanded the enzyme's optimal pH range, with the best performance observed at pH 7.5 and 8, reaching approximately 730 U g⁻¹. Additionally, increasing the temperature to 55°C led to an enhancement in the biocatalyst's hydrolytic activity, achieving a maximum of 916.24 U g⁻¹. Kinetic parameter analysis yielded values of 321.38 ± 6.31 mM for Km and 4322.46± 75.73 U g⁻¹ for Vmax. Thermal stability tests were conducted at 55°C, revealing that 83% of the biocatalyst's initial activity was retained after 24 h of exposure. Furthermore, the biocatalyst's performance in the synthesis of emollient esters (butyl oleate, 2-ethylhexyl oleate, and octyl oleate) via solvent-free esterification was evaluated. The synthesis of emollient esters demonstrated conversions exceeding 55% for octyl oleate and 2-ethylhexyl oleate at 50 and 55°C, whereas the maximum conversion for butyl oleate was 42% at 55°C. Among the bioprocesses evaluated, the synthesis of octyl oleate was selected for kinetic modeling using the ping-pong bi-bi mechanism, with five different parameter arrangements constructed. The model with the lowest corrected Akaike information criterion (AIC_C = 129.649) was selected. The findings obtained in this work open new avenues for biotechnological applications, reinforcing the relevance of the biocatalyst as a promising tool for industrial processes and scientific research. Additionally, this study provides an alternative methodology for the biochemical characterization of immobilized lipases and employs mathematical modeling to enhance the kinetic understanding of enzymatic reactions conducted at different temperatures.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1267-1280"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of GNE mutant proteins increases CHO intracellular CMP-Neu5Ac levels without impact on bioprocess performance.","authors":"John J Scarcelli, Kathryn Beal, Robert Hartsough, Jennifer Schenk, Kaffa Cote, Joanna Ross, Nhat Quach, Anand Sitaram, Xiaotian Zhong","doi":"10.1007/s00449-025-03179-5","DOIUrl":"10.1007/s00449-025-03179-5","url":null,"abstract":"<p><p>Modulation of various nucleotide sugar levels in cells has been demonstrated as an effective way to alter the composition of N-glycans. Previous studies have demonstrated the ability to impact CMP-Neu5Ac levels by the addition of N-acetylated mannosamine (ManNAc) to culture media. In this study, the relationship between adding varying levels of ManNAc to cell cultures and the impact on both CMP-Neu5Ac levels and cell growth were examined. Increasing the concentration of ManNAc added resulted in higher levels of CMP-Neu5Ac, but negatively impacted cell growth. Through cellular genetic engineering, we sought to devise an alternative method of increasing ManNAc levels without impacting cell growth. The UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase (GNE) gene is the rate-limiting enzyme in which congenital mutations can cause Sialuria, a rare metabolic disorder characterized by cytoplasmic accumulation and urinary excretion of free sialic acid. A mutant form of the GNE gene, harboring three mutations (D53H, R263I, R266Q), was site-specifically integrated (SSI) into one locus in CHO cells. This mutant protein dramatically increased the intracellular concentrations of CMP-Neu5Ac, reaching the maximal level as with the addition of ManNAc. These data together indicate that the GNE mutants could provide an effective way for substituting the high-cost supplementation of ManNAc without impacting cell growth. The investigation has also demonstrated the feasibility of the dual-landing-pad SSI cell line engineering approach for improving product qualities of biotherapeutics.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1339-1347"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly synthesis and biomedical potential of zinc oxide nanoparticles using Mentha piperita aqueous extract: comparative analysis with chemically synthesized and commercial nanoparticles.","authors":"Roberta A Dos Reis, Leonardo L da Silva, Joana C Pieretti, Benjamin Creusot, Sephora Lahouari, Gregory Francius, Ricardo A G da Silva, Igor Clarot, Ariane Boudier, Amedea B Seabra","doi":"10.1007/s00449-025-03178-6","DOIUrl":"10.1007/s00449-025-03178-6","url":null,"abstract":"<p><p>Phytosynthesis of zinc oxide nanoparticles (ZnO NPs) using Mentha piperita extract offers a sustainable alternative for biomedical applications. This study investigates the synthesis, characterization, and biological properties of biosynthesized ZnO (ZnO Bio NPs) compared to chemically synthesized (ZnO Chem NPs) and commercial ZnO (ZnO Commercial NPs). We explored how peppermint's phytochemicals influence ZnO NP synthesis and biological interactions. Peppermint-derived phytochemicals act as reducing and stabilizing agents while providing antioxidant and anticarcinogenic benefits, potentially enhancing ZnO Bio NPs' therapeutic effects. Our findings reveal that ZnO Bio NPs exhibit superior stability and bioactivity due to plant-based capping agents. ZnO Bio NPs inhibited 52% of DPPH radicals at 15.63 µg/mL, outperforming ZnO Chem and Commercial NPs. In hemocompatibility studies, ZnO Bio NPs showed minimal hemolysis, both with and without protein corona (albumin and fibrinogen), ensuring safer blood interactions. Cytotoxicity assays demonstrated that ZnO Bio NPs had an IC50 of 49.91 µg/mL in human fibroblasts, threefold less cytotoxic than ZnO Commercial NPs. These results highlight the potential of peppermint-extract-based ZnO NPs for biomedical applications, offering lower cytotoxicity and greater biocompatibility while providing an eco-friendly alternative to conventional synthesis methods.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1323-1338"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferritin-tagged ulva polysaccharide lyase for efficient degradation of biomass polysaccharides into reducing sugars.","authors":"Qing Yang, Wenhui Jin, Hua Fang, Weizhu Chen, Quanling Xie, Hui Chen, Qian Liu, Xian Jiang, Shaohua Wang, Longtao Zhang, Yiping Zhang, Zhuan Hong","doi":"10.1007/s00449-025-03177-7","DOIUrl":"10.1007/s00449-025-03177-7","url":null,"abstract":"<p><p>Enhancing the stability and the reusability of ulva polysaccharide lyase (UPL) is crucial for the efficient production of reducing sugars from ulva polysaccharides, which are vital for their broad applications in functional foods. In this study, we innovatively developed a self-immobilized UPL by fusing the enzyme with ferritin, leading to the spontaneous formation of micron-sized ulva polysaccharide lyase supraparticles (mUPLSPs). This novel system streamlines the enzyme purification and immobilization process into a single step, effectively circumventing the need for conventional, laborious chromatographic methods. The mUPLSPs exhibited superior stability and reusability, maintaining over 80% of their initial activity after five cycles of use. When compared to free UPLs, mUPLSPs displayed enhanced thermal and pH stability, resulting in a 252% increase in the yield of reducing sugars after a 40-hour reaction period. The ferritin-tagged, self-immobilization strategy not only provides a scalable and cost-efficient approach to the sustainable production of reducing sugars from ulva polysaccharides but also holds significant potential for industrial-scale applications.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1311-1321"},"PeriodicalIF":3.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}