Bioprocess and Biosystems Engineering最新文献_第2页

Clitoria ternatea flower extract assisted synthesis of Pluronic F127 and L-histidine coated SrO₂ as a multimodality nanocomposite for anti-cancer, anti-oxidant, and antimicrobial activities. 阴蒂花提取物辅助合成Pluronic F127和l -组氨酸包被SrO2作为具有抗癌、抗氧化和抗菌活性的多模态纳米复合材料。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-11-01 Epub Date: 2025-07-31 DOI: 10.1007/s00449-025-03213-6

Aakash Sharma, Suhas Ballal, Deeplata Sharma, Jaivik Pathak, AbdulAziz A AlGhamdi, Srinivas Tadepalli, Indumathi Thangavelu

{"title":"Clitoria ternatea flower extract assisted synthesis of Pluronic F127 and L-histidine coated SrO2 as a multimodality nanocomposite for anti-cancer, anti-oxidant, and antimicrobial activities.","authors":"Aakash Sharma, Suhas Ballal, Deeplata Sharma, Jaivik Pathak, AbdulAziz A AlGhamdi, Srinivas Tadepalli, Indumathi Thangavelu","doi":"10.1007/s00449-025-03213-6","DOIUrl":"10.1007/s00449-025-03213-6","url":null,"abstract":"Hepatocellular carcinoma (HepG2) is a highly aggressive liver cancer with poor prognosis, limited treatment options, and high mortality rates, making it a serious global health concern that demands urgent development of more effective and safer therapeutic approaches. In this context, the present study focuses on the green synthesis of SrO2 nanoparticles using Clitoria ternatea flower extract, followed by surface modification with Pluronic F127 (PF127) and L-histidine (LH), to fabricate SrO2-PF127-LH nanocomposites aimed at evaluating their potential anticancer efficacy against the HepG2 cell line. Various analytical techniques were used to characterize the nanocomposite, and then their anticancer activity against HePG2 liver cancer cells, antioxidant properties, and antimicrobial activity against the bacteria mentioned above were evaluated. XRD revealed the crystalline nature of SrO2 with a tetragonal phase. FTIR spectrum confirmed the Sr-O stretching band at 573 cm-1 for SrO2-PF127-LH nanocomposite. UV-visible analysis revealed the band gap energies of 4.13 eV for SrO2 and 4.07 eV for SrO2-PF127-LH nanocomposite. The surface defects including oxygen vacancies of SrO2-PF127-LH nanocomposite were investigated using PL analysis. The SrO2-PF127-LH nanocomposite exhibited excellent antibacterial and antioxidant activities when compared to SrO2 nanoparticles alone. In addition, the SrO2-PF127-LH nanocomposite had enhanced anticancer activity against liver cancer (HePG2) cell lines.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1801-1816"},"PeriodicalIF":3.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing hybrid modeling of Saccharomyces cerevisiae fermentation with mixed carbon sources and urea in a mini-stirred tank reactor. 微型搅拌槽反应器中混合碳源和尿素发酵酿酒酵母的混合建模。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-11-01 Epub Date: 2025-08-23 DOI: 10.1007/s00449-025-03222-5

Jhonatan Valencia-Velásquez, Hector Andres Yaker-Moreno, Alejandro Martínez-Guerrero, Francisco Ibáñez-Espinel, José Ricardo Pérez-Correa, Nelson H Caicedo-Ortega

{"title":"Advancing hybrid modeling of Saccharomyces cerevisiae fermentation with mixed carbon sources and urea in a mini-stirred tank reactor.","authors":"Jhonatan Valencia-Velásquez, Hector Andres Yaker-Moreno, Alejandro Martínez-Guerrero, Francisco Ibáñez-Espinel, José Ricardo Pérez-Correa, Nelson H Caicedo-Ortega","doi":"10.1007/s00449-025-03222-5","DOIUrl":"10.1007/s00449-025-03222-5","url":null,"abstract":"Saccharomyces cerevisiae is indispensable to industrial fermentation; however, many existing models fail to adequately represent the metabolic complexity of its growth on mixed carbon sources in defined media. In this study, we introduce a novel hybrid modeling framework for the batch cultivation of S. cerevisiae, utilizing sucrose, glucose, and fructose as carbon sources, and urea as a nitrogen source. The model decisively captures critical phenomena under aerobic conditions, including the Crabtree effect, diauxic shifts, and sequential sugar utilization-critical areas frequently oversimplified in current models. By integrating mechanistic kinetics with data-driven enhancements, the hybrid model significantly improves predictive accuracy relative to the purely mechanistic baseline, reducing the average prediction error by a factor of 1.9 during training and 2.0 during testing. This framework enables detailed simulation of culture dynamics and was carefully designed for modular integration into digital twin platforms and automated control systems, aligning perfectly with Industry 4.0 biomanufacturing trends. Furthermore, the model's validation under conditions pertinent to emerging bioeconomies, such as those in Latin America, underscores its industrial applicability. Overall, this work delivers a scalable and precise tool for optimizing yeast-based bioprocesses, carrying significant implications for defined media formulation, metabolic engineering, and digital fermentation technologies.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1919-1937"},"PeriodicalIF":3.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-field multiscale regulated 3D PVA-PPy conductive hydrogel for enhanced bio-electrocatalytic performance. 温度场多尺度调节三维PVA-PPy导电水凝胶增强生物电催化性能。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-13 DOI: 10.1007/s00449-025-03201-w

Xiaofei Liu, Xingshuang Liu, Qing Wen, Ye Chen

{"title":"Temperature-field multiscale regulated 3D PVA-PPy conductive hydrogel for enhanced bio-electrocatalytic performance.","authors":"Xiaofei Liu, Xingshuang Liu, Qing Wen, Ye Chen","doi":"10.1007/s00449-025-03201-w","DOIUrl":"10.1007/s00449-025-03201-w","url":null,"abstract":"Microbial fuel cells (MFCs) have been proven to be a green technology for solving energy crises, but their low power density limits their large-scale practical applications. In this paper, a three-dimensional porous composite hydrogel polyvinyl alcohol/polypyrrole (PVA/PPy) with good biocompatibility was prepared by temperature-field regulation via alternating cycles between low temperature (- 20 °C) and room temperature (25 °C) and used as the anode in MFC. The three-dimensional network structure of PPy nanospheres compressed by ice crystal stress exhibited excellent charge conduction capability and ion transport performance, which significantly improved the interfacial charge transfer efficiency of PVA/PPy-5 bioanode. Besides, the addition of PVA endowed the hydrogel with mechanical properties to resist the external forces. As the results, the maximum power density of PVA/PPy-5 MFC was 1521.04 mW/m2, which was 1.76, 2.16 and 8.32 times higher than that of PVA/PPy-0, PPy-5 and carbon felt MFCs, respectively. Such enhancement could be attributed to the combined effects of three factors, including the FT process, biocompatibility of PVA, and bioelectrocatalytic activity of polypyrrole. The high-throughput sequencing technology revealed that the PVA/PPy-5 hydrogel anode, which facilitated the selective enrichment of electrogenic microbes, played a crucial role on the regulation of functional biofilm. This work provides a new approach for developing high-performance electrodes for MFC.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1649-1660"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced succinic acid production from lignocellulosic biomass using Actinobacillus succinogenes via semi-simultaneous saccharification and fermentation. 利用琥珀酸放线菌通过半同步糖化和发酵提高木质纤维素生物质琥珀酸产量。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-24 DOI: 10.1007/s00449-025-03211-8

Dwini Normayulisa Putri, Meka Saima Perdani, Ibnu Maulana Hidayatullah, Tania Surya Utami, Kenny Lischer, Apriliana Cahya Khayrani, Muhamad Sahlan, Heri Hermansyah

{"title":"Enhanced succinic acid production from lignocellulosic biomass using Actinobacillus succinogenes via semi-simultaneous saccharification and fermentation.","authors":"Dwini Normayulisa Putri, Meka Saima Perdani, Ibnu Maulana Hidayatullah, Tania Surya Utami, Kenny Lischer, Apriliana Cahya Khayrani, Muhamad Sahlan, Heri Hermansyah","doi":"10.1007/s00449-025-03211-8","DOIUrl":"10.1007/s00449-025-03211-8","url":null,"abstract":"The utilization of semi-simultaneous saccharification and fermentation (SSSF) as the novel configuration has resulted in enhanced succinic acid (SA) production from lignocellulose biomass by Actinobacillus succinogenes. The effect of inoculum concentration, biomass type, substrate concentration, and fermentation configuration on SA production was examined in this study. The pre-hydrolysis process was applied to the pre-treated biomass for 6 h to facilitate the simultaneous saccharification and fermentation (SSF) process, which was then carried out for 48 h to achieve the SSSF configuration. According to the results, the production of SA from oil palm empty fruit bunch (OPEFB) through SSF and SSSF was 0.93 and 1.18 g/L and from sugarcane bagasse (SB) was 0.98 and 1.19 g/L, respectively. Results revealed, SSSF resulted in a 21-26% higher SA concentration compared to SSF. Furthermore, the concentration of the inoculum and substrate significantly affected the generation of SA from OPEFB but not for SB. According to this study, SSSF significantly enhanced SA production from lignocellulose biomass compared to SSF.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1755-1764"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scale-down bioreactors-comparative analysis of configurations. 缩小生物反应器-配置的比较分析。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-06-05 DOI: 10.1007/s00449-025-03182-w

Prasika Arulrajah, Anni Elina Lievonen, Dilara Subaşı, Subhashree Pagal, Dirk Weuster-Botz, Anna-Lena Heins

{"title":"Scale-down bioreactors-comparative analysis of configurations.","authors":"Prasika Arulrajah, Anni Elina Lievonen, Dilara Subaşı, Subhashree Pagal, Dirk Weuster-Botz, Anna-Lena Heins","doi":"10.1007/s00449-025-03182-w","DOIUrl":"10.1007/s00449-025-03182-w","url":null,"abstract":"In large-scale bioprocesses, gradients in pH, dissolved oxygen level (DO), and substrate concentrations can decrease bioprocess efficiency. Scale-down bioreactors, be it single stirred-tank bioreactors with a special feeding regime, multi-compartment bioreactors, or combinations of bioreactors, offer a promising lab-scale solution for comprehending these gradients, as they allow adjustment of gradients without incurring high costs. However, critical challenges arise when transitioning from large-scale to scale-down bioreactors. Chief among these is realistically approaching the gradient conditions of large-scale bioreactors and choosing appropriate scale-down bioreactor configurations. This review paper begins by addressing the gradients encountered in large-scale bioreactors. Afterward, various types of scale-down bioreactors are characterized and compared, highlighting their advantages and disadvantages. The suitability of scale-down bioreactors is analyzed by examples of bioprocesses with different microorganisms and mammalian cells to underscore the complexities inherent in scale-down bioprocesses and emphasize the influence of cellular responses to these conditions. Finally, the potential of miniaturized and microfluidic bioreactors is briefly discussed for future application in scale-down studies.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1619-1635"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process development for antifungal production by Bacillus subtilis BS20: nanoparticle supplementation, process optimization and preliminary scale-up studies. 枯草芽孢杆菌BS20抗真菌生产工艺开发：纳米颗粒补充、工艺优化和初步规模化研究。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-26 DOI: 10.1007/s00449-025-03205-6

Sikhulile N Nzimande, Isaac A Sanusi, Kwasi Yobo, Santosh O Ramchuran, Gueguim E B Kana

{"title":"Process development for antifungal production by Bacillus subtilis BS20: nanoparticle supplementation, process optimization and preliminary scale-up studies.","authors":"Sikhulile N Nzimande, Isaac A Sanusi, Kwasi Yobo, Santosh O Ramchuran, Gueguim E B Kana","doi":"10.1007/s00449-025-03205-6","DOIUrl":"10.1007/s00449-025-03205-6","url":null,"abstract":"The intensive agricultural practices used to meet global crop production demands have resulted in rigorous use of chemical pesticides. These ultimately compromise crop production as well as the environment. To alleviate these challenges, cheaper and environmentally friendly biocontrol agents have been considered as an alternative to chemical pesticides. Hence, this study was undertaken with the aim of enhancing antifungal production by Bacillus subtilis BS20 through process modeling, optimization, nanocatalysis and subsequent assessment of the scale up potential of the optimized process. The investigated process parameters included glucose concentration (10-30 g/L), incubation temperature (25-45 ℃) and incubation time (24-96 h). Optimized process conditions of 11.5 g/L glucose concentration, 24 h incubation time and 41 °C incubation temperature produced maximal antifungal activity of 68 mm zone of inhibition. Moreover, the inclusion of nanoparticles favored increased biomass yield but low antifungal activity. Additionally, constant power consumption, Reynolds number (Re) and impeller tip (Vtip) speed were implemented to scale up the antifungal production by B. subtilis BS20. Implementing constant Vtip value from the 1 L scale: 93 rpm, Re = 5.9E-04, Power (P) = 0.32 W, Power to Volume ratio (P/VL) = 160 W/m3, circulation time (tc) = 5.2 s and shear stress (γ) = 15.5 S-1, at 41 °C, gave the highest antifungal activity of 65 mm zone of inhibition in the 10 L scale bioreactor compared to the 1L bioreactors (57 mm). These findings have elucidated improved antifungal production by B. subtilis BS20 as well as provided a preliminary data for large scale production.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1707-1718"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative bacterial consortia for simulated dairy wastewater treatment: improving COD removal efficiency. 模拟乳品废水处理的创新菌群：提高COD去除效率。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-16 DOI: 10.1007/s00449-025-03202-9

Manjiri Patil, Pranav Kshirsagar, Prashant Dhakephalkar, Suneeti Gore, Vikram Lanjekar

{"title":"Innovative bacterial consortia for simulated dairy wastewater treatment: improving COD removal efficiency.","authors":"Manjiri Patil, Pranav Kshirsagar, Prashant Dhakephalkar, Suneeti Gore, Vikram Lanjekar","doi":"10.1007/s00449-025-03202-9","DOIUrl":"10.1007/s00449-025-03202-9","url":null,"abstract":"Dairy industry generates wastewater characterized by organic components, predominantly composed of proteins and fats, which can be effectively treated through biological processes. The present study aims to develop a bacterial consortium for bio-augmentation to enhance the treatment of simulated dairy wastewater. A total of 75 bacterial isolates were obtained using direct (DI) and enrichment-isolation (EI) methods. Among these, four strains exhibiting the highest proteolytic and lipolytic activities within 24 h were selected for further investigations. The isolates were screened based on their extracellular enzyme activities (proteinase and lipase), as well as their maximum lipolytic (0.3-0.7 mm/h) and proteolytic activity (0.67-0.83 mm/h) by a novel approach of rate of diffusion on TA and MSMA, respectively. The selected strains were identified by 16S rRNA gene sequencing as Massilia (DSSC1), Brevibacillus (ENAT1), Pseudomonas (ENOG5), and Lysinibacillus (ETOG2). The biodegradation potential of individual strains and their consortium was assessed through COD reduction in simulated dairy wastewater. The individual bacterial strains achieved COD reductions from an initial concentration of 3.82 g/L to 2.95, 2.81, 2.48, and 2.89 g/L. In contrast, bio-augmentation with the bacterial consortia reduced COD to 2.19 g/L, resulting in a 26-86% higher reduction compared to the individual strains. This study presents the first report on the use of a novel approach of diffusion-based assay to develop an effective and innovative bacterial consortium for efficient dairy wastewater treatment. These findings highlight the potential of this approach toward enhancing biodegradation efficiency and advancing sustainable wastewater management practice.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1661-1678"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing choline oxidase immobilization on Co₃O₄/rGO nanohybrid surface as a high-performance biosensor for diazinon detection. Co3O4/氧化石墨烯纳米杂化表面固定胆碱氧化酶作为检测重氮肼的高性能生物传感器。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-13 DOI: 10.1007/s00449-025-03206-5

Seyedeh Reyhaneh Jahandideh-Roudsari, Mostafa Shourian, Ahmad Homaei

{"title":"Developing choline oxidase immobilization on Co3O4/rGO nanohybrid surface as a high-performance biosensor for diazinon detection.","authors":"Seyedeh Reyhaneh Jahandideh-Roudsari, Mostafa Shourian, Ahmad Homaei","doi":"10.1007/s00449-025-03206-5","DOIUrl":"10.1007/s00449-025-03206-5","url":null,"abstract":"Co3O4/rGO nanoparticles were used to modify a glassy carbon electrode (GCE), where reduced graphene oxide (rGO) serves as an intermediate between graphene and graphene oxide, featuring a carbon framework enriched with oxygen-containing hydrophilic functional groups. The structural and morphological characterization of the modified electrode was carried out using Raman spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM-EDS). Electrochemical performance was evaluated through cyclic voltammetry (CV) and chronoamperometry, revealing effective electron transfer between the nanoparticles and immobilized choline oxidase (ChOx). The apparent heterogeneous electron transfer rate constants (Ks) were calculated as 0.99 s-1 for Co3O4/rGO and 5.89 s-1 for ChOx/Co3O4/rGO. The biosensor demonstrated excellent analytical performance for choline detection, with a linear response range of 5-60 µM, a sensitivity of 0.0216 µA µM-1, and a detection limit of 0.811 µM. Notably, the developed biosensor also exhibited a strong electrochemical response to the organophosphorus pesticide diazinon, indicating its potential for environmental monitoring. Given that diazinon is a widely used organophosphorus pesticide with high toxicity to humans and the environment, its sensitive detection is critical for monitoring and controlling pesticide contamination.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1719-1732"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers from a pre- fermented hardwood hydrolysate. 硬木预发酵水解产物聚（3-羟基丁酸酯-co-3-羟基戊酸酯）共聚物的制备及表征。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-18 DOI: 10.1007/s00449-025-03203-8

Warren Blunt, Purnank Shah, Vinicio Vasquez, Mengwei Ye, Christopher Doyle, Yali Liu, Sajjad Saeidlou, Fanny Monteil-Rivera

{"title":"Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers from a pre- fermented hardwood hydrolysate.","authors":"Warren Blunt, Purnank Shah, Vinicio Vasquez, Mengwei Ye, Christopher Doyle, Yali Liu, Sajjad Saeidlou, Fanny Monteil-Rivera","doi":"10.1007/s00449-025-03203-8","DOIUrl":"10.1007/s00449-025-03203-8","url":null,"abstract":"Economically viable production of poly(3-hydoxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers remains a challenge. The objective of this work was to produce low-cost PHBV copolymers from lignocellulose-derived mixed sugars without genetic engineering or addition of chemical precursors. A hardwood hydrolysate was first pre-fermented using the facultative anaerobe Propionibacterium acidipropionici, and the resulting propionate-rich effluent was used for subsequent PHBV biosynthesis in Paraburkholderia sacchari or Hydrogenophaga pseudoflava. P. acidipropionici displayed a high tolerance to the hardwood hydrolysate and produced up to 11 g L-1 propionate (with varying amounts of lactate and acetate) under batch conditions. Propionic acid exerted significant toxicity toward P. sacchari and H. pseudoflava, so dilution of the pre-fermentation effluent was required prior to the PHBV production step. When P. sacchari and H. pseudoflava were grown on the pre-fermented mixture of glucose, xylose, lactate, acetate, and propionate (diluted to 15 mM propionate), the organic acids were consumed preferentially. H. pseudoflava accumulated up to 41.7 ± 7.0% cell dry mass (CDM) as PHBV that contained 13.7 ± 2.4 mol % 3-HV subunits. Meanwhile, P. sacchari accumulated up to 56.0 ± 5.8% CDM as PHA, but with lower 3-HV contents (1.2-5.1%). The PHBV copolymers resulting from this integrated process showed a desirable crystallinity, but the molecular weights were lower and the melt temperatures were higher than expected in all cases. Future work should focus on tuning the cultivation parameters to target higher molecular weight polymers while designing a feeding strategy of the pre-fermented stream that circumvents toxicity issues and allows a better control of the formation of random vs. block copolymers.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1679-1692"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of low nitrate nitrogen availability on nutrient removal and microbial community structure in a denitrifying phosphorus removal (DPR) system. 低硝态氮有效性对反硝化除磷系统中营养物去除和微生物群落结构的影响

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-10-01 Epub Date: 2025-07-22 DOI: 10.1007/s00449-025-03208-3

Yuange Zheng, Hao Zheng, Ruitao Gao, Xin Jiang, Hongbin Zhu, Ji Zhao, Jiaxiang Nie, Xiaoxia Wang

{"title":"Impact of low nitrate nitrogen availability on nutrient removal and microbial community structure in a denitrifying phosphorus removal (DPR) system.","authors":"Yuange Zheng, Hao Zheng, Ruitao Gao, Xin Jiang, Hongbin Zhu, Ji Zhao, Jiaxiang Nie, Xiaoxia Wang","doi":"10.1007/s00449-025-03208-3","DOIUrl":"10.1007/s00449-025-03208-3","url":null,"abstract":"Combining partial nitrification and anammox with denitrifying phosphorus removal (DPR) is considered a promising strategy for nitrogen and phosphorus removal. However, the low nitrate nitrogen availability (produced from anammox) in the side-stream DPR system could affect nutrient removal and the competition between denitrifying phosphate-accumulating organisms (DPAOs) and denitrifying glycogen-accumulating organisms (DGAOs). In this study, the nitrogen and phosphorus removal performance, microbial structure shifts, and key functional groups in a DPR reactor were investigated under long-term nitrate-limited conditions. Over 205 days of DPR operation, with the nitrate concentration at the beginning of the anoxic stage gradually decreasing from 15 to 7.5 mg/L, stable and efficient nitrogen removal was maintained, while phosphorus removal efficiency reached 96.7 ± 1.6%, despite a reduction in phosphorus release amount. Microbial community analysis revealed that Candidatus_Competibacter became dominated, increasing from 2.3% to 42.2%, which contributed to efficient nitrogen removal. Meanwhile, DPAOs declined to a certain abundance but still maintained phosphorus removal performance. The result indicated that carbon and nitrate availability are the key factors driving microbial succession in the DPR system. Additionally, short-term batch tests demonstrated that the DPR system remained its capability to handle higher nitrate concentrations after long-term nitrate-limited conditions.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1733-1744"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0