Bioprocess and Biosystems Engineering最新文献

{"title":"Bioprocess biomarker identification and diagnosis for industrial mAb production based on metabolic profiling and multivariate data analysis.","authors":"Yingting Shi, Yuxiang Wan, Jiayu Yang, Yuting Lu, Xinyuan Xie, Jianyang Pan, Haibin Wang, Haibin Qu","doi":"10.1007/s00449-025-03142-4","DOIUrl":"10.1007/s00449-025-03142-4","url":null,"abstract":"<p><p>Monoclonal antibody (mAb) production is a complex bioprocess influenced by various cellular and metabolic factors. Understanding these interactions is critical for optimizing manufacturing and improving yields. In this study, we proposed a diagnostic and identification strategy using quantitative proton nuclear magnetic resonance (¹H qNMR) technology-based pharmaceutical process-omics to analyze bioprocess variability and unveil significant metabolites affecting cell growth and yield during industrial mAb manufacturing. First, batch level model (BLM) and orthogonal partial least squares-discriminant analysis (OPLS-DA) identified glucose and lactate as primary contributors to culture run variability. Maintaining an optimal glucose set point was crucial for high-yield runs. Second, a partial least squares (PLS) regression model was established, which revealed viable cell density (VCD), along with glutamine, maltose, tyrosine, citrate, methionine, and lactate, as critical variables impacting mAb yield. Finally, hierarchical clustering analysis (HCA) highlighted one-carbon metabolism metabolites, such as choline, pyroglutamate, and formate, as closely associated with VCD. These findings provide a foundation for future bioprocess optimization through cell line engineering and media formulation adjustments, ultimately enhancing mAb production efficiency.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"771-783"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of oyster protein-enhanced scaffolds with seven-band grouper muscle satellite cells for cultured seafood production.","authors":"Selvakumari Ulagesan, Sathish Krishnan, Taek-Jeong Nam, Youn-Hee Choi","doi":"10.1007/s00449-025-03148-y","DOIUrl":"10.1007/s00449-025-03148-y","url":null,"abstract":"<p><p>Cell-based aquaculture holds significant promise for revolutionizing the seafood industry by addressing the limitations of traditional fishing and aquaculture practices. In this study muscle cells from Seven-band grouper (SBG) Epinephelus septemfasciatus were isolated using an enzymatic digestion method. Initial cell attachment and growth were monitored, showing robust proliferation when cultured in L-15 medium supplemented with 10% fetal bovine serum (FBS) and basic fibroblast growth factor (bFGF). We assessed the biocompatibility and cytotoxicity of two protein sources, oyster protein (OP) and soy protein (SP), for their effects on grouper muscle cell viability and growth. OP demonstrated strong biocompatibility, effectively supporting cell viability and significantly promoting muscle cell proliferation. At a concentration of 10,000 µg/mL, OP increased muscle cell proliferation by up to 90% after 48 h of incubation. SP, however, exhibited dose-dependent cytotoxic effects, with reduced cell viability observed at higher concentrations (10,000 µg/mL) over 48 h. This comparative analysis indicates that OP maintains cellular health and enhances cell growth, while SP may limit cell viability at elevated concentrations. Following these findings, we prepared a scaffold using Alginate-κ-Carrageenan (Alg-κ-Car) combined with oyster protein (Alg-κ-Car-OP), which showed enhanced gelation and printability properties. 3D bioprinting of grouper muscle satellite cells (GMSC) within Alg-κ-Car-OP scaffolds resulted in higher cell viability than Alg-κ-Car scaffolds alone. Taste sensory analysis using an electronic tongue revealed distinct taste profiles, with Alg-κ-Car-OP-GMSC scaffolds exhibiting the highest umami score. Flavor analysis using flash gas chromatography and an electronic nose differentiated between scaffold types and protein samples, highlighting potential flavor markers. These findings underscore the potential of cell-based aquaculture, especially with OP-incorporated scaffolds, to meet the demand for sustainable and nutritious seafood alternatives. Further research is warranted to optimize production processes and explore commercial applications.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"857-875"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling up biofilm bioreactors for enhanced menaquinone-7 production.","authors":"Aydin Berenjian, Ehsan Mahdinia, Ali Demirci","doi":"10.1007/s00449-025-03155-z","DOIUrl":"https://doi.org/10.1007/s00449-025-03155-z","url":null,"abstract":"<p><p>The health benefits of menaquinone-7 (MK-7) are well-established, and its production through fermentation techniques is widespread. Our team developed an innovative biofilm reactor utilizing Bacillus subtilis natto cells to foster biofilm growth on plastic composite supports to produce MK-7. The scalability of this biofilm reactor from a 2-L benchtop scale in our laboratory and its potential for commercial applications pose significant unresolved questions. Therefore, the current research was aimed to scale up the biofilm reactor from bench scale (2-L) to the pilot scale (30-L) bioreactor. Three strategies were evaluated to understand their impact on MK-7 biosynthesis during bioreactor volume expansion: volumetric oxygen mass transfer coefficient (k_La), agitation power input per unit volume (P/V), and impeller tip velocity (V_tip). While k_La was successfully maintained during scaling, P/V and V_tip varied and were assessed for their influence on MK-7 production. After investigating these methods, it was found that the volumetric oxygen mass transfer coefficient (k_La) constant method proved to be the most effective one. The optimum MK-7 concentration achieved was 21.0 ± 1.0 mg/L, comparable to the highest MK-7 concentration of 20.6 ± 1.0 attained at the 2-L scale. This showcases the scalability of biofilm bioreactor technology and its promising potential for commercial production of MK-7. Furthermore, we explored the potential of fed-batch glucose addition to the base media in the biofilm reactor to enhance MK-7 concentration at the 30-L scale. Remarkably, results demonstrated that fed-batch strategy significantly increased MK-7 concentrations to 28.7 ± 0.3 mg/L, which made it almost 2.3-fold higher than levels produced in suspended-cell bioreactors. This finding highlights the potential of biofilm reactors as a promising replacement to the current static fermentation strategies for commercial production of MK-7.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the pretreatment and hydrolysis of a mixture of coffee husk, cowpea bean husk and cocoa pod for bacterial cellulose production.","authors":"Kátia Dos Santos Morais, Ederson Paulo Xavier Guilherme, Bruna Dos Santos Menezes, Marcus Bruno Soares Forte, Patrícia Lopes Leal, Elizama Aguiar-Oliveira","doi":"10.1007/s00449-025-03158-w","DOIUrl":"https://doi.org/10.1007/s00449-025-03158-w","url":null,"abstract":"<p><p>Agro-industrial solid residues (AISR) need to be valued as rich sources of nutrients and energy. This work aimed to obtain reducing sugars (RS) from a mixture of coffee husk (CF), cowpea bean husk (BE) and cocoa pod (CO) to produce bacterial cellulose (BC), a versatile alternative to plant cellulose. The most adequate conditions for pretreatment followed by enzymatic hydrolysis were selected with the Design of Experiments statistical tool for, respectively, a first solid load of 10% (w/v) of a mixture of CF:BE:CO = 2:8:2 in 0.25% (v/v) H₂SO₄, followed by a second solid load of 20% (w/v) in sodium citrate buffer [pH = 4.8 and] with 10 FPU/mg of cellulases. A hydrolysate was obtained, after 48 h of hydrolysis, containing 54.45 ± 2.61% (w/w) of glucose with a cellulose digestibility of almost 87%. This hydrolysate was added to nutrients and 20 g of pure glucose and was used in the cultivation of Komagataeibacter hansenii ATCC 23769 and resulted in BC = 179.00 ± 33.95 g/L (w. b.). These results encourage the biotechnological use of different AISR in mixtures to produce RS in order to obtain valuable materials, such as BC.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualization of fluorescently labeled lipase distribution characteristics at the oil-water interface.","authors":"Xian Du, Chunxiao Zhang, Biyu Peng","doi":"10.1007/s00449-025-03157-x","DOIUrl":"https://doi.org/10.1007/s00449-025-03157-x","url":null,"abstract":"<p><p>A method based on fluorescently labeled enzyme proteins was established to visualize the absorption properties of lipase at the oil-water interface, and it can be used for the effective observation of the distribution characteristics of lipase at the oil-water interface. The optimal conditions for observation include the following: oil content of 10-20% (wt%), concentration of fluorescently labelled enzyme protein of 0.25 mg/mL, reaction temperature of 25-30 °C, emulsion dispersion and stirring time of 10 min, and emulsion resting time of 30-120 s. Based on this method, a preliminary analysis of the effects of oil and lipase species on the distribution characteristics of lipase at the oil-water interface was performed. The results reveal that differences in the distributions of lipase at the oil-water interface of various fats and oils had a certain degree of correspondence with their specificity and that the distribution characteristics of the lipases on the surface of olive oil enabled effective catalytic hydrolysis to a certain extent. This method is a more objective guide for the development of lipase application technology in the fields of tanning, fur making, glue making, detergents, and sewage treatment and so on.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-assisted eco-benevolent synthesis of oxidase-mimic Cu-Mn₃O₄ as an antibacterial and antiproliferative agent.","authors":"Ankita Shome, Salim Ali, Debadrita Roy, Sangita Dey, Shilpa Sinha, Partha Barman, Anoop Kumar, Ranadhir Chakroborty, Md Salman Haydar, Swarnendu Roy, Shibaji Ghosh, Mahendra Nath Roy","doi":"10.1007/s00449-025-03149-x","DOIUrl":"https://doi.org/10.1007/s00449-025-03149-x","url":null,"abstract":"<p><p>In recent years, the marked augment of antibiotic resistance hampered the development of antibacterial agent. Nanozymes by their in situ ROS production capability oxidize cellular substances of bacterial cell and eliminate MDR bacteria. Therefore, synthesis of effective nanozymes from green precursors is rarely reported, so the prime objective of this study was to synthesize Cu-Mn₃O₄ nanozymes from aqueous extracts of medicinal plant Azadirachta indica via co-precipitation approach and to endorse their biomedical applications. The synthesized materials were characterized by X-ray diffraction (XRD), Fourier Transform Infrared spectrometer (FTIR), Scanning Electron Images (SEM), and Field-Emission Scanning Electron Microscopy (FESEM) images. X-ray Diffraction (XRD) patterns revealed the formation of hausmannite Mn₃O₄ crystal system. Fourier Transform Infrared spectrometer (FTIR) spectra revealed functional groups on the surface nanoparticles for their stabilization. Energy-Dispersive X-ray spectroscopy (EDAX) profile confirmed the existence of desired elements in the synthesized nanozymes. B1 mimics oxidase enzyme most effectively with K_m = 0.175 mM and V_max = 10.34 µM/min. The low K_m and high V_max indicates the strong binding affinity and high catalytic activity. From the agar diffusion antibacterial assay, it can be concluded that B3 is the most potent antibacterial agent specifically against Gram-positive bacteria Bacillus subtilis with inhibition zone of 27 mm at 250 µg/mL. Their cytotoxic activities on neuroblastoma (SHSY5) cell line were investigated for the first time. The data revealed that synthesized nanooctahedrons possess a significant cytotoxicity against cancer cell lines SHSY5Y (IC₅₀ = 137.47 ± 14.11 µg/mL) and SKOV3 (IC₅₀ = 72.72 ± 9.33 µg/mL). Overall, with increasing Cu amount, the percentage growth inhibition of Mn₃O₄ crystal system enhanced. The improved antibacterial activity and cytotoxicity is due to synergy between metal and phytochemicals. Radical scavenging activity of synthesized nanozymes is comparatively lower than their green source and the comparatively lower IC₅₀ values of B1, 234.12 ± 15.13 and 220.12 ± 10.37 respectively, which indicates that it is more active in scavenging DPPH and ABTS radical. B2 (IC₅₀ = 310.56 ± 5.92 µg/mL) and B3 (IC₅₀ = 43.56 ± 3.03 µg/mL) scavenge superoxide radicals and FRAP more effectively. It is noticed that synthesized nanozymes have greater antibacterial and anticancer activity but low scavenging ability compared to green extract. Thus, Cu-Mn₃O₄ NPs from Azadirachta indica leaf extract could be utilized as a replacement of potential antibiotic drug candidate against MDR bacteria and in cancer avenues.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation and detoxification of aflatoxin B1 by two peroxidase enzymes from Irpex lacteus F17.","authors":"Xiaping Xu, Peilin Lin, Yongming Lu, Rong Jia","doi":"10.1007/s00449-025-03137-1","DOIUrl":"10.1007/s00449-025-03137-1","url":null,"abstract":"<p><p>Aflatoxin B1 (AFB1), the most toxic mycotoxin produced by some Aspergillus species, is commonly found in agricultural products, especially grains, and poultry feeds. Enzymic degradation is considered to be the most promising detoxification method, because it is efficient, safe and causes minimal damage to the nutritional quality of treated foods. In this study, a recombinant manganese peroxidase (Il-MnP1) and a recombinant dye-decolorizing peroxidase (Il-DyP4) from Irpex lacteus F17 were used to degrade AFB1, either individually or in combination. The degree of degradation of AFB1 by the combined enzymes of Il-MnP1 + Il-DyP4 was higher than that of either enzyme acting alone. The half-life of AFB1 degradation by the combined enzymes was lower than that of either enzyme alone. Further analysis of the degradation products indicated that the use of the combination of Il-MnP1 + Il-DyP4 to degrade AFB1 resulted in a greater number of metabolites, including five new degradation products with the chemical formulas, C₁₆H₁₀O₈, C₁₅H₁₀O₅, C₁₅H₁₀O₆, C₁₆H₁₀O₇, and C₁₆H₈O₇. The system of Il-MnP1 + Il-DyP4 contained multiple enzyme activities that could act on different toxic sites of AFB1, thereby producing metabolites with lower toxicity and carcinogenicity, which was consistent with the results of the Ames test. These findings suggest that using the combined enzymes to convert AFB1 into non-toxic products is a good strategy for detoxifying contaminated foods and feeds.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"693-704"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanofiltration-based purification process for whole-cell transformed prebiotic galactooligosaccharides.","authors":"Anita Srivastava, Arjun Rastogi, Avijeet S Jaswal, Jatindra K Sahu, Gopal P Agarwal, Saroj Mishra","doi":"10.1007/s00449-025-03132-6","DOIUrl":"10.1007/s00449-025-03132-6","url":null,"abstract":"<p><p>The enrichment of galactooligosaccharides (GOS), synthesized by whole cells of Kluyveromyces marxianus 3551 in a 5.0-L bioreactor, was investigated in this study. The synthesized sugar mixture containing 17.89% (w/w of total carbohydrates) of GOS with 15.57% (w/w of total carbohydrates) of lactose, and 66.54% (w/w of total carbohydrates) monosaccharides as impurities, was subjected to nanofiltration for enrichment of GOS. Three distinct spiral wound membranes, namely, NFPS-01(polysulfone), NFCA-02 (cellulose acetate), and NFPES-03 (polyethersulfone) were tested out of which the NFPES-03 performed the best for fractionation of the GOS mixture. The polyethersulphone membrane (cut-off 400-1000 Da) was evaluated at 30 ℃ and 50 ℃, at different transmembrane pressures or TMP (15, 20, 25 bar) and a combination of high temperature (50 ℃) and low pressure (15 bar) gave the greatest difference in the trisaccharide and disaccharide/monosaccharide rejection percentages, resulting in enrichment of GOS. An analysis of the sugar concentrations in the retentate samples by high-performance liquid chromatography indicated the percentage recovery of GOS in the integrated process to be 88.8%. Measurement of the growth profile of several microbes on the nano-filtered GOS demonstrated its effectiveness as a prebiotic source.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"621-631"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community structure and functional characteristics in a membrane bioreactor used for real rural wastewater treatment.","authors":"Yanyan Wang, Shaoqing Su, Haojie Qiu, Liang Guo, Weihua Zhao, Yingying Qin, Chao Wang, Zhisheng Zhao, Xiang Ding, Guoli Liu, Tiantian Hu, Zenghua Wang","doi":"10.1007/s00449-025-03129-1","DOIUrl":"10.1007/s00449-025-03129-1","url":null,"abstract":"<p><p>Membrane bioreactors (MBRs) have been widely used in the field of wastewater treatment because of their small footprint and high treatment efficiency. In this research, 10 rural wastewater treatment sites in China that employ the MBR process were systematically studied. Specifically, treatment of actual domestic wastewater using MBRs was examined by high-throughput 16S rRNA gene sequencing to explore the microbial community composition and perform function prediction. The data of water quality parameters revealed high removal rates of chemical oxygen demand and NH₄⁺-N in all the sites. Proteobacteria were absolutely dominant in all the sites. Thauera, Nitrospira, Ferribacterium, and Dechloromonas were the main functional genera responsible for nitrogen and phosphorus removal at the tested sites. Nitrospira includes conventional nitrite-oxidizing bacteria and complete ammonia-oxidizing bacteria. Among them, 26 genes related to nitrogen metabolism were retrieved according to gene prediction, which verified the good NH₄⁺-N removal efficiency at the tested sites. This study focuses on the analysis of microbial community structure and functional characteristics of MBR-based treatment systems for rural wastewater treatment, thereby providing a microbial basis for improving rural wastewater treatment processes.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"577-588"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired synthesis of copper oxide nanoparticles using aqueous extracts of Cladophora glomerata (L.) Kuetz and their potential biomedical applications.","authors":"Vinay Kumar, Yadvinder Singh, Sandeep Kaushal, Ranvijay Kumar","doi":"10.1007/s00449-025-03133-5","DOIUrl":"10.1007/s00449-025-03133-5","url":null,"abstract":"<p><p>Present study deals with the green fabrication of copper oxide nanoparticles (CuO NPs) employing cell-free aqueous extract of Cladophora glomerata (L.) Kuetz, freshwater algal species. The UV-visible, FTIR, XRD, FESEM, HRTEM, EDX, BET, XPS and Raman spectroscopic techniques were used to confirm and characterize the biosynthesized CuO NPs. The UV-Vis analysis revealed a sharp peak at 264 nm with a band gap of 3.7 eV, which was attributable to the fabrication of CuO NPs. FESEM and HRTEM detect the spherical-shaped morphology with size between 40 and 50 nm. The biochemical profiling of cell free extract of the C. glomerata by Gas chromatography-mass spectrometry (GC-MS) revealed the presence of various bioactive biomolecules that may acts as a precursor for the fabrication of CuO NPs. The antibacterial study of fabricated CuO NPs revealed significant growth inhibitory potential against selected bacterial strains Klebsiella pneumoniae and Bacillus cereus with an IC₅₀ value of 10 μg/ml. The synthesized CuO NPs also displayed strong DPPH radical scavenging (IC₅₀ value 11.25 mg/L) and total antioxidant (IC₅₀ value 11 mg/L) properties. Further, the anticancer activity of fabricated CuO NPs was studied employing a human hepatocellular carcinoma (HepG2) cell line by MTT assay, which marks their ability to diminish the 50% cell with IC₅₀ value of 168.6 µg/ml. Overall, the findings confirmed that CuO NPs fabricated employing cell-free extract of C. glomerata have the potential to be used as active agent in various biomedical applications after further detailed clinical investigations.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"633-646"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}