Bioprocess and Biosystems Engineering最新文献

{"title":"Expanding the operational window of consolidated bioprocessing hydrogen production from non-detoxified alkali-pretreated tobacco stalks via microbial co-cultivation.","authors":"Ming-Hao Li, Ming-Jun Zhu, Bin-Bin Hu","doi":"10.1007/s00449-026-03330-w","DOIUrl":"https://doi.org/10.1007/s00449-026-03330-w","url":null,"abstract":"<p><p>This study investigated the feasibility of producing biohydrogen from non-detoxified alkali-pretreated tobacco stalks through separate hydrolysis and fermentation (SHF) and consolidated bioprocessing (CBP). In SHF, Thermoanaerobacterium thermosaccharolyticum MJ2 produced 215.26 ± 49.61 mM hydrogen from non-detoxified enzymatic hydrolysates, demonstrating substantial fermentative capacity under pretreatment-derived inhibitory stress. In CBP, hydrogen production by Acetivibrio thermocellus DSM1313 alone was severely inhibited by 90.71% when non-detoxified stalks were used, whereas co-cultivation with MJ2 markedly alleviated this inhibition and restored hydrogen production to 91.34% of the level obtained from detoxified stalks. To further characterize the tolerance of the co-culture system, a gradient of pretreatment liquor (0-100%, v/v) was introduced as an inhibitory stress factor. Kinetic analysis using the modified Gompertz model showed that the co-culture achieved a maximum hydrogen potential of 125.91 ± 0.54 mM at 40% (v/v) pretreatment liquor, corresponding to a 44.27% increase over the control. A hormetic effect was observed at 20% liquor concentration, whereas a critical threshold was identified at 60%, where hydrogen production sharply declined due to growth arrest of the primary cellulose degrader, DSM1313. These results demonstrate that microbial co-cultivation effectively enhances hydrogen production performance under non-detoxified conditions and expands the operational window of CBP using alkali-pretreated tobacco stalks. The beneficial effect is clearly supported at the functional level and suggests an improved consortium tolerance to pretreatment-derived inhibitory stress.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated dynamic characterization of CHO semi-perfusion processes using intensified design of experiments.","authors":"Shanshan Xu, Yuxiang Wan, Jingyu Jiao, Dong Gao, Zhenhua Chen, Haibin Wang, Haibin Qu","doi":"10.1007/s00449-026-03329-3","DOIUrl":"https://doi.org/10.1007/s00449-026-03329-3","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal dynamics in microalgae-based lead mitigation and contamination assessment in liquid biofuel production.","authors":"Jerin James, Afreen Parveen, Manisha Nanda, Sanjay Kumar, Afzal Hussain, Jin Huang, Krishna Kumar Jaiswal, Vinod Kumar, Jyoti Rawat","doi":"10.1007/s00449-026-03324-8","DOIUrl":"https://doi.org/10.1007/s00449-026-03324-8","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic metabolic engineering of Komagataella phaffii for methanol-to-lactate bioconversion.","authors":"Yujung Choi, Yejin Lin, Nam Kyu Kang","doi":"10.1007/s00449-026-03326-6","DOIUrl":"https://doi.org/10.1007/s00449-026-03326-6","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MXene-chitosan nanocomposites for structure interface and biofunctional applications in biosensing biocatalysis and regenerative biotechnology.","authors":"Lovepreet Singh, Sabin Aryal, Jagdish Verma, Aparajita Sharma, Himali Horo, Harshita Jain","doi":"10.1007/s00449-026-03328-4","DOIUrl":"https://doi.org/10.1007/s00449-026-03328-4","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing ε-poly-L-lysine production in Streptomyces albulus through tea polyphenols addition.","authors":"Di Zhang, Xiaofeng Yuan, Chang Xu, Shijie Zhu, Wenxuan Mao, Xiaoyu Zhou, Jing Bai, Liangzhi Li, Jiaolong Fu","doi":"10.1007/s00449-026-03327-5","DOIUrl":"https://doi.org/10.1007/s00449-026-03327-5","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Per- and polyfluoroalkyl substances (PFAS): persistence, toxicity, and emerging solutions.","authors":"Aris Ismanto, Tony Hadibarata, Candra Wahyuningsih, Lilik Maslukah, Yusuf Jati Wijaya, Novia Safinatunnajah, Afidhah Puspita Widyani","doi":"10.1007/s00449-026-03317-7","DOIUrl":"https://doi.org/10.1007/s00449-026-03317-7","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling of biohydrogen and polyhydroxyalkanoates production processes for next-generation biorefineries: process design, optimization and perspectives.","authors":"Amani Briki, Milos Kacanski, Romain Irague, Audrey Soric","doi":"10.1007/s00449-026-03325-7","DOIUrl":"https://doi.org/10.1007/s00449-026-03325-7","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential application of acidogenic effluent from dark fermentation process as a phosphate solubilizing organic liquid fertilizer.","authors":"Sulogna Chatterjee, Omprakash Sarkar, S Venkata Mohan","doi":"10.1007/s00449-026-03323-9","DOIUrl":"https://doi.org/10.1007/s00449-026-03323-9","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process-engineered immobilization of ureolytic biocatalysts in diatomite for in-situ biomineralization under high-alkalinity solid-phase conditions.","authors":"Guang-Zhu Zhang, Qing-Liang Xu, Hong-Feng Li, Zhuo Li","doi":"10.1007/s00449-026-03290-1","DOIUrl":"10.1007/s00449-026-03290-1","url":null,"abstract":"<p><p>Problematic clays are widely stabilized with lime to improve strength and durability, yet slow early-age strength development, pronounced brittle failure, and limited densification often constrain performance in high-alkalinity environments. This study explores a synergistic route that integrates diatomite-immobilized ureolytic microbially induced calcium carbonate precipitation with lime stabilization. Mixtures were prepared with 6% hydrated lime and 3 to 7% diatomite or a diatomite-based microbial curing agent; where applicable a 1.0 M urea-calcium chloride solution supplied substrates for mineralization. Mechanical properties were assessed by unconfined compressive strength, unconsolidated-undrained triaxial testing, and ultrasonic pulse velocity, and microstructure and phase assemblage were characterized using scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis. Results show clear dosage-dependent gains. At 28 d, unconfined compressive strength reached 1987.18, 2278.17, and 2563.00 kPa for 3%DE-B, 5%DE-B, and 7%DE-B, exceeding the corresponding diatomite-only groups by 75.65%, 83.17%, and 88.50%. Under 300 kPa confining pressure, cohesion increased to 382.52 to 498.72 kPa and the internal friction angle to 38.88 to 47.88°. Ultrasonic pulse velocity rose with curing age, with 7%DE-B increasing by 45.01% from 7 d to 28 d. Triaxial responses followed linear elasticity, strain hardening, peak strength, and softening, while failure shifted from through-crack brittleness to non-through diffuse cracking with pronounced bulging. Microstructural evidence indicates clustered calcium silicate hydrate (C-S-H) progressively encapsulating diatomite and abundant ellipsoidal CaCO₃ forming interconnected three-dimensional networks. These observations support a synergy between pozzolanic reaction and microbial mineralization that constructs a multi-scale cementation network, densifies the matrix, and strengthens interparticle contacts, yielding reproducible improvements in strength, ductility, and structural integrity. These results provide reference value for performance enhancement and process optimization of lime-stabilized clays and cementation-enhanced clay systems.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"859-876"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}