Bioprocess and Biosystems Engineering最新文献_第2页

Current strategies for rutin nano-formulation; a promising bioactive compound with increased efficacy. 芦丁纳米配方研究现状一种有前景的生物活性化合物，具有更高的功效。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-03-28 DOI: 10.1007/s00449-025-03156-y

Maryam Malekpour, Alireza Ebrahiminezhad, Zeinab Karimi, Mahdiyar Iravani Saadi, Aydin Berenjian

{"title":"Current strategies for rutin nano-formulation; a promising bioactive compound with increased efficacy.","authors":"Maryam Malekpour, Alireza Ebrahiminezhad, Zeinab Karimi, Mahdiyar Iravani Saadi, Aydin Berenjian","doi":"10.1007/s00449-025-03156-y","DOIUrl":"10.1007/s00449-025-03156-y","url":null,"abstract":"Rutin is an herbal polyphenolic compound recognized for its numerous therapeutic benefits, including antioxidant, anticancer, and antimicrobial properties. However, its application in biomedical fields encounters significant challenges, such as low solubility, poor absorption, low bioavailability, short half-life, and rapid metabolism. In recent years, advancements in nanotechnology have presented promising solutions to these limitations. Consequently, various nano-formulation strategies have been developed to enhance rutin's solubility, absorption, and overall efficacy. These strategies can be broadly categorized into two approaches. The first involves transforming rutin into nanocrystals without the use of any secondary compounds. The second approach entails nano-formulating rutin with other compounds, including proteins, polysaccharides, lipids, polymers, and metals. This study offers a review of these approaches and their applications in biomedical sciences, focusing on their categories, preparation methods, and biomedical properties. Initially, the pharmacological potential of rutin, its application in recent clinical trials, and its mechanisms of action are outlined. Next, it explores how nano-carriers can enhance rutin's bioavailability. Subsequently, the types of nanostructures employed are categorized, along with a discussion of their fabrication methods and benefits. In some instances, certain drawbacks are also reported. The data provided can guide the selection of optimal strategies for rutin nano-formulation, based on the intended biomedical application.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"877-898"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the impact of polydopamine hydrogel electrodes with various doping methods on the performance of microbial fuel cells. 研究不同掺杂方式的聚多巴胺水凝胶电极对微生物燃料电池性能的影响。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-04-19 DOI: 10.1007/s00449-025-03154-0

Jinhu Ma, Ye Chen, Qing Wen

{"title":"Research on the impact of polydopamine hydrogel electrodes with various doping methods on the performance of microbial fuel cells.","authors":"Jinhu Ma, Ye Chen, Qing Wen","doi":"10.1007/s00449-025-03154-0","DOIUrl":"10.1007/s00449-025-03154-0","url":null,"abstract":"Microbial fuel cells (MFCs) have attracted considerable interest as a promising bioelectrochemical technology for directly converting chemical energy into electrical energy. However, their performance remains limited by the properties of anode materials and their interactions with microbial communities. In this study, PPy-MXene/PDA and PDA-PPy-MXene composite hydrogel electrodes (PMP and PPM) were fabricated on a conductive carbon felt substrate to systematically evaluate the influence of different PDA doping strategies on electrode performance. The PMP electrode exhibited a maximum power density of 3.62 W/m2, which represented a 34.6% increase compared to the PPM electrode (2.69 W/m2). Moreover, the protein content on the PMP electrode reached 38.05 ± 4.88 mg/cm2, 3.79 times higher than that on the PPM electrode (10.05 ± 3.05 mg/cm2). High-throughput sequencing of the 16S rRNA gene revealed that the relative abundance of Geobacter on the PMP electrode surface reached 73.66%, significantly higher than the 51.17% observed on the PPM electrode. These results are attributed to the PDA doping method involving secondary deposition on the electrode surface. This method optimizes the electron transfer pathways and significantly enhances the electrode's conductivity and electrochemical activity by altering the surface roughness of the electrode and increasing the content of hydrophilic functional groups. Consequently, it significantly promotes the enrichment of electroactive microorganisms and improves the efficiency of extracellular electron transfer. This study optimized PDA doping strategies to significantly enhance the electrochemical performance of MFCs, providing new insights and approaches for the rational design of high-performance bioelectrochemical electrodes.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"951-970"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-purpose Bacillus subtilis fermentation: enhanced nattokinase production via oxygen-enriched fed-batch cultivation and natto starter preparation from harvested biomass. 双重用途枯草芽孢杆菌发酵：通过富氧分批培养和从收获的生物质制备纳豆发酵剂来增强纳豆激酶的生产。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-05-01 DOI: 10.1007/s00449-025-03151-3

Jiawen Zheng, Yaping Sun, Yunyu Liao, Peng Qin, Rongzhen Che, Jing-Yi Zhao, Zijun Xiao

{"title":"Dual-purpose Bacillus subtilis fermentation: enhanced nattokinase production via oxygen-enriched fed-batch cultivation and natto starter preparation from harvested biomass.","authors":"Jiawen Zheng, Yaping Sun, Yunyu Liao, Peng Qin, Rongzhen Che, Jing-Yi Zhao, Zijun Xiao","doi":"10.1007/s00449-025-03151-3","DOIUrl":"10.1007/s00449-025-03151-3","url":null,"abstract":"Nattokinase (NK) is one of the most important functional components in natto, but its content is low. In this study, the fermentation conditions using Bacillus subtilis JZ08-02 for high-yield NK production were investigated, and the residual bacterial pellets were used to prepare a natto starter. Batch fermentation of NK was conducted using a 5 L fermenter, and soybean milk and glucose were used as the substrates. When the stirring speed was increased from 450 to 650 rpm with air supply at 1.0 vvm, NK was increased from 4859 ± 142 to 12,294 ± 226 IU/mL. When pure oxygen was supplied, 15,013 ± 550 IU/mL of NK was obtained. When fed-batch fermentation was conducted, the titer was further elevated to 18,014 ± 112 IU/mL, which was increased by about 76% compared with the previous result. The experimental findings revealed that aeration control and nutrient feeding regimens exerted pronounced effects on NK productivity during submerged fermentation. The crude enzyme supernatant was obtained by centrifugation and the precipitate was collected. With optimized protectant, the bacterial pellets were freeze-dried with 90.1% cell survival rate. Using economical and edible feedstocks, this study achieved a significant enhancement in NK fermentation yield via oxygen-enriched fed-batch cultivation. At the same time, a natto starter was prepared as a by-product using the residual cell waste.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1039-1046"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesized ZnO-based bimetallic nanocomposite for anticancer, antimicrobial, and photocatalytic applications. 生物合成zno基双金属纳米复合材料在抗癌、抗菌和光催化方面的应用。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-03-23 DOI: 10.1007/s00449-025-03150-4

Nidal M Hussein, Sobhan Mortazavi-Drazkola

{"title":"Biosynthesized ZnO-based bimetallic nanocomposite for anticancer, antimicrobial, and photocatalytic applications.","authors":"Nidal M Hussein, Sobhan Mortazavi-Drazkola","doi":"10.1007/s00449-025-03150-4","DOIUrl":"10.1007/s00449-025-03150-4","url":null,"abstract":"Industrial wastewater, particularly antibiotic-laden effluents, poses a significant environmental threat, necessitating efficient and sustainable remediation strategies. In this study, ZnO/Au nanocomposites were synthesized using Urtica dioica extract (ZnO/Au@UDE NCs), offering an eco-friendly alternative to conventional chemical methods. The NCs exhibited well-defined spherical and oval morphologies (40-50 nm), as confirmed by FTIR, TEM, and XRD analyses. Their photocatalytic efficiency in degrading penicillin G was optimized by adjusting key parameters, achieving rapid degradation within 130 min. The incorporation of gold nanoparticles significantly enhanced the electron-hole separation, thereby improving photocatalytic performance. Furthermore, ZnO/Au@UDE NCs demonstrated potent antibacterial activity against Escherichia coli and Pseudomonas aeruginosa (MIC: 125 µg/ml) and exhibited strong antioxidant and anticancer properties, with IC50 values of 72.49 µg/ml for MCF-7 and 23.63 µg/ml for AGS cancer cells. The combined photocatalytic and biological functionalities highlight the potential of these NCs for environmental remediation and biomedical applications, demonstrating a sustainable and multifunctional approach to nanomaterial development.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"913-926"},"PeriodicalIF":3.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferritin-tagged ulva polysaccharide lyase for efficient degradation of biomass polysaccharides into reducing sugars. 铁蛋白标记的ulva多糖裂解酶有效地将生物质多糖降解为还原糖。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-28 DOI: 10.1007/s00449-025-03177-7

Qing Yang, Wenhui Jin, Hua Fang, Weizhu Chen, Quanling Xie, Hui Chen, Qian Liu, Xian Jiang, Shaohua Wang, Longtao Zhang, Yiping Zhang, Zhuan Hong

{"title":"Ferritin-tagged ulva polysaccharide lyase for efficient degradation of biomass polysaccharides into reducing sugars.","authors":"Qing Yang, Wenhui Jin, Hua Fang, Weizhu Chen, Quanling Xie, Hui Chen, Qian Liu, Xian Jiang, Shaohua Wang, Longtao Zhang, Yiping Zhang, Zhuan Hong","doi":"10.1007/s00449-025-03177-7","DOIUrl":"https://doi.org/10.1007/s00449-025-03177-7","url":null,"abstract":"Enhancing the stability and the reusability of ulva polysaccharide lyase (UPL) is crucial for the efficient production of reducing sugars from ulva polysaccharides, which are vital for their broad applications in functional foods. In this study, we innovatively developed a self-immobilized UPL by fusing the enzyme with ferritin, leading to the spontaneous formation of micron-sized ulva polysaccharide lyase supraparticles (mUPLSPs). This novel system streamlines the enzyme purification and immobilization process into a single step, effectively circumventing the need for conventional, laborious chromatographic methods. The mUPLSPs exhibited superior stability and reusability, maintaining over 80% of their initial activity after five cycles of use. When compared to free UPLs, mUPLSPs displayed enhanced thermal and pH stability, resulting in a 252% increase in the yield of reducing sugars after a 40-hour reaction period. The ferritin-tagged, self-immobilization strategy not only provides a scalable and cost-efficient approach to the sustainable production of reducing sugars from ulva polysaccharides but also holds significant potential for industrial-scale applications.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of GNE mutant proteins increases CHO intracellular CMP-Neu5Ac levels without impact on bioprocess performance. 表达GNE突变蛋白增加CHO细胞内CMP-Neu5Ac水平，但不影响生物工艺性能。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-25 DOI: 10.1007/s00449-025-03179-5

John J Scarcelli, Kathryn Beal, Robert Hartsough, Jennifer Schenk, Kaffa Cote, Joanna Ross, Nhat Quach, Anand Sitaram, Xiaotian Zhong

{"title":"Expression of GNE mutant proteins increases CHO intracellular CMP-Neu5Ac levels without impact on bioprocess performance.","authors":"John J Scarcelli, Kathryn Beal, Robert Hartsough, Jennifer Schenk, Kaffa Cote, Joanna Ross, Nhat Quach, Anand Sitaram, Xiaotian Zhong","doi":"10.1007/s00449-025-03179-5","DOIUrl":"https://doi.org/10.1007/s00449-025-03179-5","url":null,"abstract":"Modulation of various nucleotide sugar levels in cells has been demonstrated as an effective way to alter the composition of N-glycans. Previous studies have demonstrated the ability to impact CMP-Neu5Ac levels by the addition of N-acetylated mannosamine (ManNAc) to culture media. In this study, the relationship between adding varying levels of ManNAc to cell cultures and the impact on both CMP-Neu5Ac levels and cell growth were examined. Increasing the concentration of ManNAc added resulted in higher levels of CMP-Neu5Ac, but negatively impacted cell growth. Through cellular genetic engineering, we sought to devise an alternative method of increasing ManNAc levels without impacting cell growth. The UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase (GNE) gene is the rate-limiting enzyme in which congenital mutations can cause Sialuria, a rare metabolic disorder characterized by cytoplasmic accumulation and urinary excretion of free sialic acid. A mutant form of the GNE gene, harboring three mutations (D53H, R263I, R266Q), was site-specifically integrated (SSI) into one locus in CHO cells. This mutant protein dramatically increased the intracellular concentrations of CMP-Neu5Ac, reaching the maximal level as with the addition of ManNAc. These data together indicate that the GNE mutants could provide an effective way for substituting the high-cost supplementation of ManNAc without impacting cell growth. The investigation has also demonstrated the feasibility of the dual-landing-pad SSI cell line engineering approach for improving product qualities of biotherapeutics.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly synthesis and biomedical potential of zinc oxide nanoparticles using Mentha piperita aqueous extract: comparative analysis with chemically synthesized and commercial nanoparticles. 薄荷水萃取物氧化锌纳米粒子的生态合成及其生物医学潜力：与化学合成纳米粒子和商业纳米粒子的比较分析。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-24 DOI: 10.1007/s00449-025-03178-6

Roberta A Dos Reis, Leonardo L da Silva, Joana C Pieretti, Benjamin Creusot, Sephora Lahouari, Gregory Francius, Ricardo A G da Silva, Igor Clarot, Ariane Boudier, Amedea B Seabra

{"title":"Eco-friendly synthesis and biomedical potential of zinc oxide nanoparticles using Mentha piperita aqueous extract: comparative analysis with chemically synthesized and commercial nanoparticles.","authors":"Roberta A Dos Reis, Leonardo L da Silva, Joana C Pieretti, Benjamin Creusot, Sephora Lahouari, Gregory Francius, Ricardo A G da Silva, Igor Clarot, Ariane Boudier, Amedea B Seabra","doi":"10.1007/s00449-025-03178-6","DOIUrl":"https://doi.org/10.1007/s00449-025-03178-6","url":null,"abstract":"Phytosynthesis of zinc oxide nanoparticles (ZnO NPs) using Mentha piperita extract offers a sustainable alternative for biomedical applications. This study investigates the synthesis, characterization, and biological properties of biosynthesized ZnO (ZnO Bio NPs) compared to chemically synthesized (ZnO Chem NPs) and commercial ZnO (ZnO Commercial NPs). We explored how peppermint's phytochemicals influence ZnO NP synthesis and biological interactions. Peppermint-derived phytochemicals act as reducing and stabilizing agents while providing antioxidant and anticarcinogenic benefits, potentially enhancing ZnO Bio NPs' therapeutic effects. Our findings reveal that ZnO Bio NPs exhibit superior stability and bioactivity due to plant-based capping agents. ZnO Bio NPs inhibited 52% of DPPH radicals at 15.63 µg/mL, outperforming ZnO Chem and Commercial NPs. In hemocompatibility studies, ZnO Bio NPs showed minimal hemolysis, both with and without protein corona (albumin and fibrinogen), ensuring safer blood interactions. Cytotoxicity assays demonstrated that ZnO Bio NPs had an IC50 of 49.91 µg/mL in human fibroblasts, threefold less cytotoxic than ZnO Commercial NPs. These results highlight the potential of peppermint-extract-based ZnO NPs for biomedical applications, offering lower cytotoxicity and greater biocompatibility while providing an eco-friendly alternative to conventional synthesis methods.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of antioxidant, antibacterial, anticancer and wound healing properties of eco-friendly synthesized copper oxide nanoparticles from Plumeria rubra leaf extract. 黄花鸡蛋花叶提取物合成的环保氧化铜纳米颗粒的抗氧化、抗菌、抗癌和伤口愈合性能研究。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-21 DOI: 10.1007/s00449-025-03176-8

Annapoorani Angusamy, Subramanian Palanisamy, Sangeetha Ravi, Manikandan Kumaresan, Sonaimuthu Mohandoss, SangGuan You, Manikandan Ramar

{"title":"Investigation of antioxidant, antibacterial, anticancer and wound healing properties of eco-friendly synthesized copper oxide nanoparticles from Plumeria rubra leaf extract.","authors":"Annapoorani Angusamy, Subramanian Palanisamy, Sangeetha Ravi, Manikandan Kumaresan, Sonaimuthu Mohandoss, SangGuan You, Manikandan Ramar","doi":"10.1007/s00449-025-03176-8","DOIUrl":"https://doi.org/10.1007/s00449-025-03176-8","url":null,"abstract":"Plants serve as a rich source of bioactive agents and coupling them with carriers using nanotechnology has recently become an effective therapeutic approach in pharmacognosy. Metal oxides, especially copper oxide (CuO), have been employed in synthesizing nanoparticles due to their efficient reducing properties. The purpose of this work was to examine the physicochemical, antioxidant, antibacterial, anticancer, and wound healing abilities of copper oxide nanoparticles (CuONPs) synthesized using Plumeria rubra leaf extract. FTIR, XRD, FESEM, EDX, AFM, and UV-vis spectroscopy were used to confirm the formation of CuONPs, and the results showed that they were spherical in shape and 35 nm in size. DPPH and nitric oxide antioxidant assays revealed that they possess effective free radical scavenging ability. CuONPs showed bactericidal activity against human pathogenic bacteria. The anticancer effect of CuONPs was assessed on the Neuro-2a (N2a) neuroblastoma cells. Both P. rubra leaf extract and CuONPs exhibited dose-dependent cytotoxicity with morphological distortions and apoptosis, along with a loss of membrane integrity. In vivo analysis of CuONPs for their wound healing ability in Wistar albino rats showed a better wound closure percentage compared to that of the control animals. Based on our findings, CuONPs may be applied as a potential therapeutic agent in developing treatments for a spectrum of various diseases.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and bioactive functionalization of bioinks for 3D bioprinting. 用于3D生物打印的生物墨水的遗传和生物活性功能化。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-20 DOI: 10.1007/s00449-025-03180-y

Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Nagaraju Sunnam, Anil Sindhu, Seepana Praveenkumar

{"title":"Genetic and bioactive functionalization of bioinks for 3D bioprinting.","authors":"Pawan Kumar, Jitender Sharma, Ravinder Kumar, Jan Najser, Jaroslav Frantik, Nagaraju Sunnam, Anil Sindhu, Seepana Praveenkumar","doi":"10.1007/s00449-025-03180-y","DOIUrl":"https://doi.org/10.1007/s00449-025-03180-y","url":null,"abstract":"3D bioprinting is revolutionizing tissue engineering and regenerative medicine by enabling the precise fabrication of biologically functional constructs. At its core, the success of 3D bioprinting hinges on the development of bioinks, hydrogel-based materials that support cellular viability, proliferation, and differentiation. However, conventional bioinks face limitations in mechanical strength, biological activity, and customization. Recent advancements in genetic engineering have addressed these challenges by enhancing the properties of bioinks through genetic modifications. These innovations allow the integration of stimuli-responsive elements, bioactive molecules, and extracellular matrix (ECM) components, significantly improving the mechanical integrity, biocompatibility, and functional adaptability of bioinks. This review explores the state-of-the-art genetic approaches to bioink development, emphasizing microbial engineering, genetic functionalization, and the encapsulation of growth factors. It highlights the transformative potential of genetically modified bioinks in various applications, including bone and cartilage regeneration, cardiac and liver tissue engineering, neural tissue reconstruction, and vascularization. While these advances hold promise for personalized and adaptive therapeutic solutions, challenges in scalability, reproducibility, and integration with multi-material systems persist. By bridging genetics and bioprinting, this interdisciplinary field paves the way for sophisticated constructs and innovative therapies in tissue engineering and regenerative medicine.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440. 通过删除工程恶臭假单胞菌KT2440中乙酰丙酸转录调控因子（lvaR），提高了微生物乙酰丙酸的产量。

IF 3.5 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2025-05-19 DOI: 10.1007/s00449-025-03175-9

Hyun Jin Kim, Byung Chan Kim, Gaeun Lim, Yebin Han, Yunhee Jeong, Hee Taek Kim, Woo-Young Jeon, Jungoh Ahn, Shashi Kant Bhatia, Yung-Hun Yang

{"title":"Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440.","authors":"Hyun Jin Kim, Byung Chan Kim, Gaeun Lim, Yebin Han, Yunhee Jeong, Hee Taek Kim, Woo-Young Jeon, Jungoh Ahn, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.1007/s00449-025-03175-9","DOIUrl":"https://doi.org/10.1007/s00449-025-03175-9","url":null,"abstract":"Levulinic acid (LA) is a platform compound regarded as a promising organic intermediate for the synthesis of various chemicals such as fuel additives, plasticizers, solvents, and pharmaceuticals. Traditionally, LA is produced via acid-catalyzed dehydration and hydrolysis of lignocellulosic biomass, but this process involves challenges such as high temperatures and pressures, the use of strong acids, byproducts formation, and limitations in recovery and purification. To provide an alternative for chemical synthesis, we previously designed an integrated process to produce LA from glucose using genetically engineered Pseudomonas putida KT2440. However, as the consumption of the produced LA could not be completely prevented, its overall yield was limited. Therefore, in this study we constructed P. putida strains with additional knock-out of the lva operon genes (lvaAB, lvaE, and lvaR) in a pcaIJ knock-out strain, and introduced the aroG, asbF, and adc genes to design an LA production pathway. The pcaIJ, lvaR double knock-out strain P. putida HP205 produced 20.42 mM of LA from glycerol, and culture condition including temperature, glucose concentration, and nitrogen source were optimized. Under optimal conditions, P. putida HP205 produced 73.9 mM (8.58 g/L) LA in fed-batch fermentation. When crude glycerol was used as the substrate, both LA production and cell growth were enhanced. This study presents the impact of the LA transcriptional regulator and demonstrates a strategy for enhanced LA production in P. putida.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0