Bioprocess and Biosystems Engineering最新文献

{"title":"Nutrient composition, microbial diversity, and functional prediction of Baijiu distiller's grains fermented feed via solid state fermentation at different temperatures.","authors":"Chun Wang, Xia Wang, Ling Ao, Shan Zeng, Jianping Yang, Mengyan Wang, Caihong Shen, Yingxue Sun","doi":"10.1007/s00449-026-03288-9","DOIUrl":"10.1007/s00449-026-03288-9","url":null,"abstract":"<p><p>The utilization of Baijiu distiller's grains (BDGs), commonly used as a feed ingredient, remains limited due to challenges such as elevated levels of anti-nutritional factors (ANFs), high fiber content, and low protein concentration. Microbial fermentation has been recognized as an effective strategy to improve the nutritional quality of feed substrates. However, its efficacy is highly dependent on process parameters such as temperature. Despite its significance, the effect of temperature on the interaction between microbial communities and the physicochemical properties of BDGs during fermentation remains poorly understood. To address this gap, BDGs were fermented using Bacillus subtilis, Candida utilis, and Geotrichum candidum under varying temperatures. Comprehensive physicochemical analyses combined with high-throughput sequencing of microorganisms were performed to investigate the dynamic changes in both fermentation products and microbial kinetics. The results revealed that the optimal fermentation performance was achieved at 34 °C. At this temperature, compared to untreated BDGs, phytic acid, tannin, and crude fiber levels were significantly reduced by 65.18%, 30.69%, and 15.34% respectively, whereas crude protein and amino acid contents increased by 15.5% and 7.13%. Furthermore, temperature was found to play a crucial role in shaping the dynamics of microbial community succession, with Stenotrophomonas, Bacillus, Pseudomonas, Paenibacillus, and Pediococcus identified as key bacterial genera influencing the nutritional composition of BDGs. Temperature variations drive shifts in microbial communities and consequently affect the nutritional quality during fermentation. These findings provide valuable experimental evidence and support the potential of fermented BDGs as a viable protein ingredient substitute in animal feed.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"831-843"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammonia stripping by in situ biogas self-circulation to mitigate ammonia Inhibition in high-solids anaerobic digestion of chicken manure.","authors":"Xinhui Yu, Shaojie Bi, Haipeng Wang, Fangtong Wei, Chunshuang Wang, Jiaxin Lu, Changjiang Zhao, Yanjie Wang","doi":"10.1007/s00449-026-03297-8","DOIUrl":"10.1007/s00449-026-03297-8","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"953-963"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146256813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined pretreatment of biogas slurry immersion followed by freeze-thaw cycle for methane production enhancement of rice straw.","authors":"Zhongjiang Wang, Xiwei Chen, Ziang Zhu, Lili Wang, Yu Zheng, Junwei Wang, Dandan Zong","doi":"10.1007/s00449-026-03298-7","DOIUrl":"10.1007/s00449-026-03298-7","url":null,"abstract":"<p><p>The challenge associated with agricultural straw pretreatment before anaerobic digestion (AD) lies in overcoming biomass recalcitrance at a low cost while minimizing the wastewater that requires additional purification. Biogas slurry from AD itself, rich in ammonia nitrogen and microorganisms, can pretreat straw instead of ammonia reagents or microbial agents. This study explored a synergistic pretreatment approach, particularly relevant in cold regions that offer natural cold resources, involving the immersion of straw in biogas slurry, followed by freeze-thaw cycles. Research findings indicated that freeze-thaw treatment significantly enhanced the lignin removal of biogas slurry immersion. Although sealing offered advantages for biogas slurry pretreatment, open was also feasible in terms of lignin removal and volatile fatty acids production, which helps further reduce operating costs. The hydrolytic acidification mediated by microorganisms, such as Clostridium sensu stricto 1 and Comamonas, dominated the biogas slurry immersion process, rather than the ammonolysis driven by free ammonia. The delignification process of rice straw underwent significantly interactive effects by several factors, reaching maximal lignin removal rates of 63.27% and 39.16% under sealed and open immersions, which corresponded to the optimal pretreatment conditions: immersion temperature of 30.70 and 43.20 °C, immersion durations of 4 days and 20 h, biogas slurry-to-straw ratios of 16.33:1 and 14.62:1, both followed by four freeze-thaw cycles. The specific methane yields under the two optimal conditions reached 281.37 and 262.18 L/kg VS. The combined pretreatment presents a promising low-cost operational strategy, especially in cold regions, while also facilitating on-site utilization of biogas slurry.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"965-984"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocessing of tasar cocoon degumming water for sericin extraction: structural, thermal, and biofunctional characterization.","authors":"Karmabeer Jena, Ananta Sinha, Jabed Akhtar, N B Chowdary","doi":"10.1007/s00449-026-03296-9","DOIUrl":"10.1007/s00449-026-03296-9","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"941-952"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in amine-based pretreatment for the degradation of lignocellulosic biomass and its application in microbial fermentation for chemical production.","authors":"Jiabin Wang, Cheng Zhang, Yu Shao, Fang Xie, Rongling Yang, Hongzhen Luo","doi":"10.1007/s00449-025-03259-6","DOIUrl":"10.1007/s00449-025-03259-6","url":null,"abstract":"<p><p>Lignocellulosic biomass, a renewable raw material for producing biobased products, has attracted significant attention in biorefinery. To efficient deconstruct the complex components in lignocellulose, development of pretreatment technologies is a key research focus. Practically, use of cost-effective and easily recyclable solvent to achieve high fractionation and enzymatic hydrolysis efficiency could prompt the valorization of lignocellulose. With the excellent nucleophilicity and reactivity, amine compounds have shown remarkable advantages in lignocellulose pretreatment. Hence, this review summarizes recent amine-based pretreatment strategies to explore their key roles in lignocellulose structural deconstruction, enhancement of sugar yields, and fermentability of substrates for biobased chemical production. Firstly, the effects of amine compounds on lignin removal, cellulose crystalline structure transformation, and improvement in enzymatic hydrolysis are reviewed. Subsequently, progress in synergistic pretreatment combining amine solvents with green systems such as ionic liquids, surfactants, and mechanical assistance is summarized, demonstrating the potential of integrated approach to enhance biomass fractionation, preserve lignin structure, and increase fermentable sugar yields. Meanwhile, this review also discusses the application of solid and liquid fractions obtained from amine-based pretreatment in microbial fermentation to produce bioethanol, L-lactic acid, and biobutanol. Finally, challenges remain in by-product control and solvent recovery in amine-based pretreatment, and prospects for its development in future biorefinery are systematically provided. Overall, amine-based pretreatment offers a sustainable pathway for efficient deconstruction and valorization of lignocellulose.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"759-775"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145494431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing nutrient removal efficiency and biomass yield in Oedogonium: controlling nitrogen and phosphorus concentration, light-dark ratio, and initial biomass loading.","authors":"Jiaqing Xiong, Jiaxing Xu, Jiajia Zhou, Zhenyao Wang, Feichi Xun, Yanzheng Liu, Qionghua Zhang","doi":"10.1007/s00449-026-03303-z","DOIUrl":"10.1007/s00449-026-03303-z","url":null,"abstract":"<p><p>Reclaimed water with elevated nitrogen (N), phosphorus (P), and organics triggers eutrophication in replenished landscape waters. Conventional remediation methods face challenges of complexity and high costs. Filamentous algae ponds (FAPs) efficiently absorb N and P and produce harvestable biomass. Oedogonium is particularly adept at converting nutrients in reclaimed water into algal biomass. The effects of these cultivation conditions on Oedogonium sp. nutrient assimilation, photosynthetic parameters, and biomass recovery were evaluated with batch experiments using varying light-dark ratios (L: D ratios), nitrogen-phosphorus concentrations and their ratios (N/P mass ratios of 7, 14, 21, 28, and 35), and initial biomass loading. Specifically, Oedogonium sp. exhibits an optimal N/P mass ratio of 14. At a P concentration of 1 mg-P L^- 1, N removal efficiency reaches 0.56 mg-N L^- 1 d^- 1, with protein yield at 4.17 mg L^- 1 d^- 1 and polysaccharide yield at 11.21 mg L^- 1 d^- 1. However, when P concentration increases to 1.5 mg-P L^- 1, N removal efficiency decreases to 0.50 mg-N L^- 1 d^- 1. Correlation analysis indicates that N and P removal efficiency peaks when Oedogonium sp. is harvested on day 12. Furthermore, increasing the initial biomass loading does not yield better results. When the biomass is 1.5 g L^- 1 and the L: D ratio is 16:8, higher nutrient removal efficiency and biomass yields can be achieved. This study refined the theoretical framework governing the regulation of Oedogonium sp. growth by nutrients, light-dark ratios, and initial biomass, providing fundamental principles and regulatory thresholds for achieving efficient nutrient removal and biomass production in FAPs.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1031-1045"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of nitrogen availability and forms on the degradation pathway of lignocellulose by Bacillus subtilis.","authors":"Yanan Yu, Yixin Hao, Yaoxing Zhang, Wenxing He, Nayanci Portal-Gonzalez, Ramon Santos-Bermudez, Wenbo Wang","doi":"10.1007/s00449-026-03294-x","DOIUrl":"10.1007/s00449-026-03294-x","url":null,"abstract":"<p><p>Nitrogen availability and forms significantly influence the efficiency of microbial lignocellulose degradation. Using Bacillus subtilis LP-10, a strain with strong lignocellulose-degrading capacity, as a model, we evaluated the effects of three concentration gradients inorganic nitrogen sources (NaNO₃, NH₄Cl, NH₄NO₃) on lignocellulose degradation pathway. By conducting a 30-day microcosm experiment simulating the decomposition of Larch needle litter and measured litter mass-loss rate, the activities of cellulolytic and ligninolytic enzymes, and the residual lignocellulose components. Under low nitrogen conditions (N1), transcriptome sequencing was performed for all three nitrogen sources and the control to identify differentially expressed CAZyme genes; Key targets were validated by RT-qPCR. The results indicate that low nitrogen concentrations promote lignocellulose degradation, whereas higher concentrations exert the opposite effect. NH₄Cl-N1 was most effective, yielding the highest mass-loss rate (9.823%), significant increases in six key lignocellulose-degrading enzymes, and the greatest reductions in residual lignocellulose. Transcriptomics showed that NH₄Cl triggered 1,101 differentially expressed genes, with enrichment in two-component systems, glycolysis/gluconeogenesis, and ABC transporters. Within CAZymes, GH1 genes (bglC, bglA, bglH, GYO_RS25640) and AA genes (cotA, glcD) were significantly upregulated under NH₄Cl-N1; RT-qPCR corroborated these trends. Correlation analysis further demonstrated significant positive associations between the expression of bglH and GYO_RS25640 correlated positively with β-glucosidase, filter paperase, and lignin peroxidase activities, and negatively with hemicellulose and lignin contents. Overall, this study revealed the regulatory mechanisms by which nitrogen form and concentration modulate lignocellulose degradation pathways in Bacillus subtilis LP-10, providing important references for the microbial-mediated utilization of lignocellulosic biomass resources in forest litter.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"905-923"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TiO₂-based adsorption platform for non-invasive capture and quantification of cell-derived secretome biomarkers.","authors":"Jesus Jimenez-Osorio, Carolina Rivera-Crespo, William Meza-Morales, Maria J Buendia-Otero, Maria Ramos-Alamo, Deisy Jimenez-Corzo, Sahimy Ayus-Martinez, Maribella Domenech, Camilo Mora","doi":"10.1007/s00449-026-03306-w","DOIUrl":"10.1007/s00449-026-03306-w","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1047-1062"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic application of sludge biochar: achieving optimal pollution treatment and greenhouse gas suppression in constructed wetlands.","authors":"Xueting Zhang, Yanqin Yang, Zhi Rao, Jianguo Zhao, Haiming Wu, Shubiao Wu, Yuran Song, Jingjing Du","doi":"10.1007/s00449-026-03302-0","DOIUrl":"10.1007/s00449-026-03302-0","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1015-1029"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.","authors":"Tsung-Yu Tseng, Hsin-Ju Mai, Shuo-Ting Yan, Hsing-Fen Tsai, Yun Chen, Chao-Ling Yao","doi":"10.1007/s00449-026-03300-2","DOIUrl":"10.1007/s00449-026-03300-2","url":null,"abstract":"","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"985-997"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13124967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}