Bioprocess and Biosystems Engineering最新文献

{"title":"Cost-effective sewage-powered microbial fuel cells with nitrogen-doped cobalt carbon nanofiber cathodes and biomass-derived graphitized anodes.","authors":"Nasser A M Barakat, Radwan A Almasri, Osama M Irfan","doi":"10.1007/s00449-025-03134-4","DOIUrl":"10.1007/s00449-025-03134-4","url":null,"abstract":"<p><p>This study presents the design and performance of microbial fuel cells (MFCs) utilizing sewage water as a renewable source for electricity generation. The proposed MFCs employ an air-cathode, single-chamber configuration that harnesses atmospheric oxygen as the electron acceptor, eliminating the need for consumable electron acceptor chemicals. Unlike traditional systems, no external microorganisms are introduced; instead, indigenous microbial communities present in sewage are utilized as efficient biocatalysts. The anode is constructed from graphitized corncob, a biomass-derived material that surpasses conventional anodes such as carbon cloth and carbon paper, achieving power densities of 450 ± 15 mW/m², outperforming 120 ± 7 and 105 ± 5 mW/m² of conventional anodes. For the cathode, N-doped and Co-incorporated carbon nanofibers (CNFs) are employed, representing a cost-effective alternative to precious metal-based catalysts. This cathode material demonstrates superior electrochemical performance, producing a power density of 750 ± 17 mW/m², a notable improvement over the Pt/C cathode. Optimization studies identified 5 wt% CNFs as the ideal loading for the cathode. These findings underscore the viability of this MFC configuration in harnessing sewage water for sustainable electricity generation while reducing costs and reliance on precious metals.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"647-663"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial, antibiofilm, and antiurease activities of green-synthesized Zn, Se, and ZnSe nanoparticles against Streptococcus salivarius and Proteus mirabilis.","authors":"Sumeyra Gurkok, Murat Ozdal, Tuba Cakici, Esabi Basaran Kurbanoglu","doi":"10.1007/s00449-025-03130-8","DOIUrl":"10.1007/s00449-025-03130-8","url":null,"abstract":"<p><p>This study assesses the antimicrobial, antibiofilm, and antiurease properties of selenium (Se), zinc (Zn), and zinc selenide (ZnSe) nanoparticles (NPs) against clinically pathogenic strains of Streptococcus salivarius and Proteus mirabilis. The Se, Zn, and ZnSe NPs, synthesized by Pseudomonas aeruginosa OG1, were characterized using transmission electron microscopy (TEM) revealing average sizes of approximately 30 ± 10 nm, 30 ± 15 nm, and 40 ± 10 nm, respectively. Atomic force microscopy (AFM) was used to examine the morphological and topological characteristics of the NPs. The structural and crystal characteristics were investigated using X-ray diffraction (XRD). Among the evaluated NPs, Zn NPs at a concentration of 200 mg/mL exhibited the most substantial growth inhibition zone against S. salivarius. Conversely, the highest antibiofilm activity was observed against P. mirabilis, notably with 200 µg/mL Zn NPs. In the context of antiurease activity, both 100 μg Zn and ZnSe NPs caused complete urease inhibition (100%) in P. mirabilis within the initial 5 h, with notable inhibition rates of 94% and 80%, respectively, observed against S. salivarius. Significantly, in the current landscape of NP research primarily focused on antimicrobial and antibiofilm properties, our study stands out due to its pioneering exploration of antiurease activities with these NPs. This distinctive emphasis on antiurease effects contributes original and unique value to our study, offering novel insights into the broader spectrum of NP applications, and paving the way for potential therapeutic advancements.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"589-603"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering of Escherichia coli for enhanced production of p-coumaric acid via L-phenylalanine biosynthesis pathway.","authors":"Chorok Jeong, Seung Hee Han, Chang Gyu Lim, Sun Chang Kim, Ki Jun Jeong","doi":"10.1007/s00449-025-03128-2","DOIUrl":"10.1007/s00449-025-03128-2","url":null,"abstract":"<p><p>p-Coumaric acid (p-CA), an invaluable phytochemical, has novel bioactivities, including antiproliferative, anxiolytic, and neuroprotective effects, and is the main precursor of various flavonoids, such as caffeic acid, naringenin, and resveratrol. Herein, we report the engineering of Escherichia coli for de novo production of p-CA via the PAL-C4H pathway. As the base strain, we used the E. coli H-02 strain, which was previously engineered for sufficient supplementation of L-phenylalanine, the main precursor of p-CA. For the bioconversion of L-Phe to p-CA, we constructed and optimized an expression system for phenylalanine ammonia lyase (SmPAL), codon-optimized cinnamate 4-hydroxylase (AtC4H), and its redox partner, cytochrome P450 reductase (AtCPR1). We confirmed that the engineered cell showed higher production of p-CA at 30 °C and the addition of 0.5 mM 5-aminolevulinic acid could increase the production titer further. Subsequently, the main pathways of acetic acid (poxB and pta-ackA) were eliminated to reduce its accumulation and restore cell growth. Next, to increase the available pool of cofactor (NADPH), the co-expression system of the zwf gene in the pentose phosphate pathway (PPP) was integrated into genome and the expression level was optimized with synthetic promoters. Finally, by optimizing fed-batch culture in a 5 L-scale bioreactor, the engineered strain achieved 1.5 g/L p-CA with a productivity of 31.8 mg/L/h.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"565-576"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient removal performance and microbial composition analysis in hybrid membrane bioreactor for municipal wastewater treatment.","authors":"Kamran Tari, Mohammad Reza Samarghandi, Reza Shokoohi, Ghorban Asgari, Eskandar Poorasgari, Pezhman Karami, Saeid Afshar","doi":"10.1007/s00449-025-03135-3","DOIUrl":"10.1007/s00449-025-03135-3","url":null,"abstract":"<p><p>The removal of nutrients from wastewater to reduce the toxicity of these compounds to the environment requires more space in wastewater treatment plants to establish anaerobic, anoxic and aerobic treatment stages. To address this limitation, researchers have developed practical, intensive hybrid treatment systems that enhance nutrient removal performance while requiring less space. However, the implementation of hybrid systems within a reactor introduces the interaction between the attached and suspended growth that can influence the microbial community structure and the performance of the system, so it is crucial to understand the composition of the microbial communities involved in hybrid growth to optimize control strategies in these systems. This study investigated the microbial community structure of the integrated moving bed membrane bioreactor (IMBMBR) system and its impact on nutrient removal in municipal wastewater. The findings demonstrated that the effluent quality was improved with the IMBMBR. The efficiency of removing COD, BOD₅, <math> <mrow><msubsup><mtext>NH</mtext> <mrow><mn>4</mn></mrow> <mo>+</mo></msubsup> <mtext>-N</mtext></mrow> </math> and <math> <mrow><msubsup><mtext>PO</mtext> <mrow><mn>4</mn></mrow> <mrow><mn>3</mn> <mo>-</mo></mrow> </msubsup> <mtext>-P</mtext></mrow> </math> in the IMBMBR were 91 ± 4.0%, 95 ± 4.0%, 99 ± 0.2% and 24 ± 3.0%, respectively. The IMBMBR had better nitrite oxidation and complete nitrification by increasing the diversity and abundance of effective bacteria. The abundance of Proteobacteria, Bacteroidetes and Nitrospira was enhanced in IMBMBR. Coexistence of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in IMBMBR led to increased nutrient removal. The study results suggest that IMBMBR can be an effective process for nutrient removal, achieving quality standards that comply with legal requirements for wastewater in municipal and industries with limited space for establishing treatment facilities. Additionally, this process can be quickly implemented as an upgrade to existing wastewater treatment plants, avoiding the need to develop an entirely new system.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"665-678"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesized ZnO and ZnO-based composites for wound healing applications.","authors":"Abdul Wafi, Mohammad Mansoob Khan","doi":"10.1007/s00449-024-03123-z","DOIUrl":"10.1007/s00449-024-03123-z","url":null,"abstract":"<p><p>In recent years, zinc oxide nanoparticles (ZnO NPs) have gained much attention in biomedical applications because of their distinctive physicochemical features such as low toxicity and biocompatible properties. Traditional methods to produce ZnO NPs sometimes include harmful substances and considerable energy consumption, causing environmental issues and potential health risks. Nowadays, the concern of ZnO production has moved toward environmentally friendly and sustainable synthesis methods, using natural extracts or plant-based precursors. This review discusses the green synthesis of ZnO NPs utilizing various plant extracts for wound healing applications. Moreover, ZnO NPs have antibacterial characteristics, which can prevent infection, a substantial obstacle in wound healing. Their ability to maintain inflammation, proliferation, oxidative stress, and promote angiogenesis proves their critical role in wound closure. In addition, ZnO NPs can also be easily and ideally incorporated with wound dressings and scaffolds such as hydrogel, chitosan, cellulose, alginate, and other materials, due to their exceptional mechanical properties. The latest publication of green synthesis of ZnO NPs and their applications for wound healing has been discussed. Therefore, this review provides a current update of knowledge on the sustainable and biocompatible ZnO NPs for specific applications, i.e., wound healing applications. In addition, the green synthesis of ZnO NPs using plant extracts also provides a particular approach in terms of material preparation, which is different from previous review articles.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"521-542"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.","authors":"Jingchun Sun, Xing Jiang, Feng Xu, Xiwei Tian, Ju Chu","doi":"10.1007/s00449-025-03136-2","DOIUrl":"10.1007/s00449-025-03136-2","url":null,"abstract":"<p><p>To prevent the unique difficulty of hygromycin-based gene editing on industrial A. niger strain and increase the working efficiency, the local pyrG marker was removed by well-designed dual sgRNAs and repair template through Cas9-ribonucleoprotein (RNP) strategy in this study. The positive rate of the desired pyrG auxotroph construction was 100%, while no transformant was observed using the traditional methods. The complementation strain showed similar fermentation character as the starting strain. Moreover, an efficient and seamless knock out plasmid-based strategy was established, achieving positive rate at 90% and 50% for challenging Δku70 and Δku80 respectively. Further, combined hygromycin markers and miniaturization cultivation were conducted to select the poor growth strain. Finally, skillfully designed sgRNA and amdS counter-selection repair template were used to obtain ERG3^Tyr185 mutation. A highly-efficient precise strategy was established for A. niger through a diagnostic PCR method, with nearly 100% positive rate. Highly- precise desired point mutation was achieved by the developed gene toolbox.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"679-691"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recycling the recyclers: strategies for the immobilisation of a PET-degrading cutinase.","authors":"Stefanie Fritzsche, Marcus Popp, Lukas Spälter, Natalie Bonakdar, Nicolas Vogel, Kathrin Castiglione","doi":"10.1007/s00449-025-03131-7","DOIUrl":"10.1007/s00449-025-03131-7","url":null,"abstract":"<p><p>Enzymatic degradation of polyethylene terephthalate (PET) represents a sustainable approach to reducing plastic waste and protecting fossil resources. The cost efficiency of enzymatic PET degradation processes could be substantially improved by reusing the enzymes. However, conventional immobilisation strategies, such as binding to porous carriers, are challenging as the immobilised enzyme can only interact with the macromolecular solid PET substrate to a limited extent, thus reducing the degradation efficiency. To mitigate this challenge, this work compared different immobilisation strategies of the PET-degrading cutinase ICCG_DAQI. Immobilisation approaches included enzyme fixation via linkers to carriers, the synthesis of cross-linked enzyme aggregates with different porosities, and immobilisation on stimulus-responsive polymers. The highest degradation efficiencies were obtained with the pH-responsive material Kollicoat^®, where 80% of the initial enzyme activity could be recovered after immobilisation. Degradation of textile PET fibres by the cutinase-Kollicoat^® immobilisate was investigated in batch reactions on a 1 L-scale. In three consecutive reaction cycles, the product yield of the released terephthalic acid exceeded 97% in less than 14 h. Even in the fifth cycle, 78% of the maximum yield was achieved in the same reaction time. An advantage of this process is the efficient pH-dependent recovery of the immobilisate after the reaction, which integrates seamlessly into the terephthalic acid recovery by lowering the pH after hydrolysis. This integration therefore not only simplifies the downstream processing, but also provides a cost-effective and resource-efficient solution for both enzyme reuse and product separation after PET degradation, making it a promising approach for industrial application.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"605-619"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of torularhodin production by Rhodotorula glutinis through the stimulation of physicochemical stress and application of the bioproduct as an additive in the food industry.","authors":"Júlio Gabriel Oliveira de Lima, Nathalia Vieira Porphirio Veríssimo, Caio de Azevedo Lima, Flávio Pereira Picheli, Ariela Veloso de Paula, Valéria de Carvalho Santos-Ebinuma","doi":"10.1007/s00449-024-03126-w","DOIUrl":"10.1007/s00449-024-03126-w","url":null,"abstract":"<p><p>Carotenoids are pigments responsible for the red-orange colorations in valuable food products, and they can be produced via biotechnological means through microorganisms. Beyond their role as natural colorants, some carotenoids offer significant health benefits due to their antioxidant properties, making them valuable nutritional additives in the food industry. However, obtaining these compounds from natural sources with high quantity and purity poses challenges which reduces its market share when produced through a biotechnological route. This study proposes utilizing nutritional and physical stress to enhance carotenoid production, specifically torularhodin, using the yeast Rhodotorula glutinis CCT-2186. A Design of Experiments approach identified malt extract as the most suitable nitrogen source for maximizing carotenoid production. Furthermore, introducing a surfactant (Tween 80) in the culture medium, and extending the cultivation time to 96 h, led to an increase in torularhodin production, reaching a notable 2.097 mg/mL (377,68% more when compared to the initial condition) under the best condition [(%w/v): dextrose (1), KH₂PO₄ (0.052), MgSO₄.7H₂O (0.052) and NH₄NO₃ (0.4), malt extract with a pH of 5.0/ 96 h/30 °C]. Lastly, to demonstrate the viability of utilizing the carotenoid extract as a food colorant, it was applied in edible gelatin. These findings highlight the critical role of nutritional, physical, and mechanical stresses in optimizing torularhodin production, particularly the conversion of γ-carotene to torularhodin by R. glutinis.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"543-563"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current strategies for rutin nano-formulation; a promising bioactive compound with increased efficacy.","authors":"Maryam Malekpour, Alireza Ebrahiminezhad, Zeinab Karimi, Mahdiyar Iravani Saadi, Aydin Berenjian","doi":"10.1007/s00449-025-03156-y","DOIUrl":"https://doi.org/10.1007/s00449-025-03156-y","url":null,"abstract":"<p><p>Rutin is an herbal polyphenolic compound recognized for its numerous therapeutic benefits, including antioxidant, anticancer, and antimicrobial properties. However, its application in biomedical fields encounters significant challenges, such as low solubility, poor absorption, low bioavailability, short half-life, and rapid metabolism. In recent years, advancements in nanotechnology have presented promising solutions to these limitations. Consequently, various nano-formulation strategies have been developed to enhance rutin's solubility, absorption, and overall efficacy. These strategies can be broadly categorized into two approaches. The first involves transforming rutin into nanocrystals without the use of any secondary compounds. The second approach entails nano-formulating rutin with other compounds, including proteins, polysaccharides, lipids, polymers, and metals. This study offers a review of these approaches and their applications in biomedical sciences, focusing on their categories, preparation methods, and biomedical properties. Initially, the pharmacological potential of rutin, its application in recent clinical trials, and its mechanisms of action are outlined. Next, it explores how nano-carriers can enhance rutin's bioavailability. Subsequently, the types of nanostructures employed are categorized, along with a discussion of their fabrication methods and benefits. In some instances, certain drawbacks are also reported. The data provided can guide the selection of optimal strategies for rutin nano-formulation, based on the intended biomedical application.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.","authors":"Fucheng Zhu, Zixu Yan, Jingli Dai, Juwen Wang, Shiping Huang, Jingbo Ma, Naidong Chen, Yongjun Zang","doi":"10.1007/s00449-025-03153-1","DOIUrl":"https://doi.org/10.1007/s00449-025-03153-1","url":null,"abstract":"<p><p>Salidroside has been widely utilized in the food and cosmetics industries. However, the efficient synthesis of salidroside remains a challenge. In this study, a potential uridine diphosphate-dependent glycosyltransferase (UGT) from Bacillus subtilis 168 (named UGT_BS) was identified through evolutionary relationship analysis and molecular docking, with findings subsequently validated by experimental verification. The optimal conversion of UGT_BS for salidroside synthesis reached 98.4% (mol/mol). Additionally, a conversion exceeding 85% (mol/mol) was achieved using the UGT_BS-AtSuSy cascade reaction with tyrosol concentrations ranging from 1 to 10 mM, demonstrating the substrate tolerance of UGT_BS at high concentrations. Kinetic determination and molecular docking confirmed that the strengthened hydrogen bonds and suitable active center conformation between the enzyme and substrate may account for the efficient synthesis of salidroside. Furthermore, 43.5 mM of salidroside was obtained using a fed-batch cascade reaction strategy. The UGT_BS identified in this study shows significant potential for salidroside synthesis, and the strategy employed here serves as a reference for the discovery of related enzymes.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}