Bioprocess and Biosystems Engineering最新文献

{"title":"Plasma substitute combination enhances in vitro expansion of blood cells by modulating redox status and signaling pathways.","authors":"Guofeng Zhang, Ruisheng Xu, Huimin Huang, Yuanyuan Zhao, Wen-Song Tan, Haibo Cai","doi":"10.1007/s00449-025-03204-7","DOIUrl":"10.1007/s00449-025-03204-7","url":null,"abstract":"<p><p>Plasma is the primary microenvironment, where red blood cells (RBCs) survive and function, with its components playing crucial roles in erythroid expansion and RBC functionality. This study aims to elucidate the relationship between the combination of critical components in plasma and the expansion and cell state of erythroid cells. Using Design of Experiment (DOE) methods, we screened and optimized the concentrations of plasma components that significantly impact the in vitro expansion of TF-1 cells. We identified a plasma substitute combination composed of hypoxanthine, dexamethasone, and vitamin B complex and, significantly enhancing TF-1 cell expansion in the serum-free medium supplemented with bovine serum albumin by 1012.41 folds, compared to 327.50 folds in the negative control. In addition, the proportion of CD34⁺ cells in the medium supplemented with this combination was 54.77%, comparable to the negative control, while hemoglobin expression was 0.64 pg/cell, significantly higher than that of the negative control. Given that various components of this formulation affect intracellular redox status and signaling pathway activation, we further investigated these aspects. Cells cultured with this combination showed improved mitochondrial membrane potential, lower intracellular reactive oxygen species (ROS) levels, reduced apoptosis rates, and enhanced STAT5 phosphorylation. These results indicated that the plasma substitute combination improves intracellular redox status and activates the JAK/STAT signaling pathway in TF-1 cells. This study provides valuable insights for developing serum-free media for the in vitro expansion of erythroid cells.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1693-1705"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fed-batch lipid production by Rhodosporidium toruloides-7191 using wood hydrolysate.","authors":"Lachi Wankhede, Bridget Signe Price, Carlos Saul Osorio-González, Rahul Saini, Satinder Kaur Brar","doi":"10.1007/s00449-025-03209-2","DOIUrl":"10.1007/s00449-025-03209-2","url":null,"abstract":"<p><p>The shift toward sustainable biofuels and bioproducts has increased interest in microbial production systems using renewable substrates. This study explores the use of wood hydrolysate, an abundant, cost-effective lignocellulosic substrate, as the primary carbon source for lipid and carotenoid production by Rhodosporidium toruloides-7191 under fed-batch cultivation in a 3-L bioreactor. The fed-batch strategy, chosen over batch and continuous modes, enables controlled nutrient supply, minimizes substrate inhibition, and maintains a favorable carbon-to-nitrogen ratio, thereby supporting prolonged biosynthesis and higher product yields. The process achieved a maximum lipid production of 22.33 g/L, a total lipid accumulation of 57.9% and a total carotenoid production of 4.23 mg/L. Fatty acid analysis shows a composition rich in linoleic acid (C18:2), oleic acid (C18:1), and palmitic acid (C16:0), indicating its suitability for biodiesel applications. The results emphasize R. toruloides-7191 as a promising candidate for industrial-scale applications, advancing sustainable production of biofuels and high-value bioproducts. The effectiveness of wood hydrolysate as a substrate further supports the feasibility of this approach, highlighting its potential in advancing industrial-scale processes for the production of biofuels and value-added compounds.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1745-1753"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher butanol titer and selectivity in electro-fermentation experiments with Clostridium acetobutylicum ATCC 824 are due mainly to methyl viologen rather than electrode polarization.","authors":"Edouard Beudy, Sandra Menir, Michel Laurent, Fadhel Ben Chaabane, Théodore Bouchez, Hélène Velly","doi":"10.1007/s00449-025-03212-7","DOIUrl":"10.1007/s00449-025-03212-7","url":null,"abstract":"<p><p>Butanol selectivity is crucial for the development of an industrial process targeting butanol as the main product of acetone-butanol-ethanol (ABE) fermentation by solventogenic Clostridia. This study evaluated electro-fermentation (EF), with an electron carrier, methyl viologen (MV), as a strategy to modify solvent production in Clostridium acetobutylicum ATCC 824 under optimized batch fermentation conditions. Cathodic EF was performed with or without 0.5 mM MV in single-compartment reactors with two-chamber and a negative potential was applied to the cathode. The greatest differences in final products concentrations were observed with the addition of MV compared to the control and EF fermentations. The fermentation with MV only was slowed down and resulted in 11% more butanol (control: 14.2 g.L^-1, MV: 15.8 g.L^-1) and half as much acetone, increasing the butanol/acetone (B/A) ratio by 137% from 1.93 to 4.57 g C.g C^-1. The pH profile was also modified with a final pH 0.5 unit higher. Conversely, EF did not appear to alter product formation significantly, even if slight differences were observed under our conditions. In addition, the reductase activity (RA) was measured by flow cytometry and showed that EF alone affected the intracellular redox state compared to the control fermentation, as did the addition of MV. However, electrode polarization had no effect on the extracellular oxidoreduction potential (ORP) profiles.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1765-1777"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of precursor MicroRNA (pre-miRNA) as a powerful tool for robust CHO production cell line platform development.","authors":"Li Gao, Xiaohong Li, Mengxin Zhang, Bin Li, Xiuxiu Miao, Chao Yu, Wei Guo, Cuiqiao Zhang, Xiangyuan He, Kaisheng Huang, Zichen Qian","doi":"10.1007/s00449-025-03200-x","DOIUrl":"10.1007/s00449-025-03200-x","url":null,"abstract":"<p><p>Chinese hamster ovary (CHO) cells are the most widely used host for the commercial production of recombinant therapeutic proteins. The rapidly growing demand for large quantities of biologics at controllable cost-of-goods requires continuous cell engineering and process optimization of the CHO host cells. MicroRNAs (miRNAs) have been shown to enhance recombinant protein production in CHO cells. While studies have demonstrated that transient overexpression of certain miRNAs can increase recombinant protein yields, systematic comparisons of different miRNA overexpression forms (primary, precursor, and mature) remain limited. Furthermore, their application in stable cell line development, particularly for difficult-to-express proteins, has yet to be thoroughly explored. This study evaluated three miRNA overexpression strategies: primary miRNAs (pri-miRNAs), precursor miRNAs (pre-miRNAs), and flanked mature miRNAs (incorporating the mature sequence plus reverse complementary and loop sequences), to enhance the expression of difficult-to-express proteins in stable CHO cell lines. Notably, these miRNA constructs were built-in with the gene of interest (GOI) on the same vector to simplify stable cell line generation. Our results indicate that the pre-miRNA overexpression strategy is the most effective. Overexpression of premiR-92a, premiR-200a, premiR-483, and premiR-106b significantly increased the expression level of a bispecific antibody (BsAb) and an Fc-fusion protein without compromising product quality. Further clone evaluation of the premiR-92a and premiR-483 overexpression groups revealed an improved proportion of high-productivity and stable clones. In conclusion, this study demonstrates that integrating pre-miRNA expression cassettes into therapeutic protein vectors for co-expression is a valuable and effective engineering strategy for developing a robust stable CHO expression platform.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1637-1647"},"PeriodicalIF":3.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of short-chain fatty acids production by biochar-supported zero-valent iron in anaerobic fermentation: focusing on metabolic reactions and electron transport.","authors":"Zhifang Ning, Jiale Liu, Jiaxing Zhang, Weizhang Zhong, Tianqi Yang, Yali Huang, Xue Qin, Xiaoxu Zhang, Xingdan Xu","doi":"10.1007/s00449-025-03236-z","DOIUrl":"https://doi.org/10.1007/s00449-025-03236-z","url":null,"abstract":"<p><p>Carbon-based and iron-based materials have been widely reported as effective promoters in biogas fermentation due to the promotion of electron transport. However, the effect of these materials, especially in combination, on short-chain fatty acids (SCFAs) production has been scarcely reported. In this study, the production of short-chain fatty acids (SCFAs) from green cabbage waste was promoted by adding biochar (BC) and biochar-supported zero-valent iron (BC@ZVI). The underlying mechanisms, focusing on metabolic pathways and electron transport, were subsequently investigated through metagenomic analysis. The optimal SCFAs yields were achieved with BC (5 g·L⁻1) and BC@ZVI (15 g·L⁻1). While BC notably enhanced n-butyrate production (89.4-fold), BC@ZVI balancedly promoted acetate and n-butyrate. Metagenomics revealed that BC@ZVI's superiority stemmed from its enhanced ability to enrich functional microbes and facilitate electron transfer. Metagenomic analysis revealed that BC@ZVI enriched Sphaerochaeta and Herbinix, which could participate in the direct interspecies electron transfer process. The abundance of almost all functional enzymes involved in carbohydrate hydrolysis and the synthesis of acetate and n-butyrate were remarkably increased by BC@ZVI. BC and BC@ZVI lead to a notable enrichment of conductive pili genes, including pilB, pilC, and pilM. BC@ZVI enriched both conductive pili and c-type cytochromes, which could be considered a more effective selection than BC. Notably, BC@ZVI was more effective than BC in stimulating n-butyrate-type fermentation, significantly shortening the lag phase and the overall fermentation cycle, thereby exhibiting better comprehensive performance, enhancing pH buffering capacity, and strengthening electron transfer and substrate hydrolysis. The results proved the potential of BC@ZVI in SCFAs fermentation and deciphered the underlying mechanisms, which provided a new perspective to promote resource recovery of organic waste by anaerobic system.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zika virus-like particle production in a stirred tank bioreactor using a baculovirus/insect cell system.","authors":"Thaissa Consoni Bernardino, Milena Miyu Teruya, Paulo Eduardo da Silva Cavalcante, Vinícius Aragão Tejo Dias, Júlia Públio Rabello, Fernanda Angela Correia Barrence, Jaci Leme, Luis Giovani de Oliveira Guardalini, Aldo Tonso, Soraia Attie Calil Jorge, Eutimio Gustavo Fernández Núñez","doi":"10.1007/s00449-025-03233-2","DOIUrl":"https://doi.org/10.1007/s00449-025-03233-2","url":null,"abstract":"<p><p>Zika virus (ZIKV) was declared a public health emergency in 2016, yet effective vaccines are still needed. Among the immunization platforms under evaluation, virus-like particles (VLP) are promising candidates. Growth, metabolism, and respiration are among the cell host processes that are essential for optimizing and characterizing VLP upstream production stage. These cell functions can be influenced by factors such as culture medium composition and the multiplicity of infection (MOI) in viral vector-based expression systems. This study investigated the effects of three MOIs (2, 6, and 10) in a baculovirus/Sf9 insect cell system on ZIKV VLP production with and without medium supplemented with 0.028 mM cholesterol and 6 nM albumin. Medium supplementation during the growth phase increased the cell growth rate from 0.357 × 10⁴ to 0.565 × 10⁴ <math><mfrac><mtext>cells</mtext> <mrow><mtext>mL</mtext> <mo>·</mo> <mtext>h</mtext></mrow> </mfrac> </math> . In addition, cholesterol and albumin supplementation increased the expression of ZIKV structural proteins during infection. Higher MOIs led to increased substrate uptake and metabolite production, suggesting intensified cellular metabolism. Western blot analysis revealed that under nonsupplemented conditions, the highest MOI resulted in increased ZIKV envelope production, with a maximum protein concentration range of 1.049 <math><mfrac><mtext>mg</mtext> <mtext>L</mtext></mfrac> </math> higher when comparing 6 to 2 <math><mfrac><mtext>PFU</mtext> <mtext>cell</mtext></mfrac> </math> MOI via SDS‒PAGE densitometry. However, a lower MOI, 2 <math><mfrac><mtext>PFU</mtext> <mtext>cell</mtext></mfrac> </math> , might be advantageous when a supplemented medium is used, which upper limit for ZIKV envelope protein concentration was 1.834 <math><mfrac><mtext>mg</mtext> <mtext>L</mtext></mfrac> </math> higher than that from the nonsupplemented assay in semiquantitative analysis, which reached 23.504 <math><mfrac><mtext>mg</mtext> <mtext>L</mtext></mfrac> </math> of ZIKV envelope protein. The resulting VLP had an average diameter of ~ 60 nm, making them suitable for vaccine applications.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and effective treatment of drug-resistant Pseudomonas aeruginosa biofilm using flavonoid naringin.","authors":"Subramanian Palaniappan, Vinothini Gunasekaran, Thavasumani Muthu, Lekha Sree Venkatesan, Palanivel Sathishkumar","doi":"10.1007/s00449-025-03232-3","DOIUrl":"https://doi.org/10.1007/s00449-025-03232-3","url":null,"abstract":"<p><p>Pseudomonas aeruginosa infections and biofilms are difficult to treat due to their remarkable ability of antibiotic resistance. Consequently, the incidence of drug-resistant P. aeruginosa infection cases is steadily increasing in hospitals worldwide. Herein, the frequency of drug-resistant P. aeruginosa wound infection cases among hospitalized patients is examined. In addition, suitable natural components with potent antibacterial activity against the drug-resistant P. aeruginosa strains are screened. Among 27 specimens, three P. aeruginosa strains (including carbapenem and multidrug-resistant) are isolated. The antibacterial efficacy of natural components, such as curcumin, naringin, quercetin, tannic acid, and rutin, is evaluated against the isolated drug-resistant P. aeruginosa strains. Comparatively, naringin showed great antibacterial potential against drug-resistant P. aeruginosa strains. The zone of inhibition is found between 13 and 16 mm for naringin (20 mM) against P. aeruginosa strains; while, the minimum inhibitory concentration is found between 10 and 14 mM. A complete eradication of bacterial cells in P. aeruginosa mature biofilm is achieved by naringin at 28 mM within 24 h. Naringin interacts with the most important amino acids found in the MexR and RlpA, which confirms its role in the targeted treatment of drug-resistant P. aeruginosa. Remarkably, naringin is found to be hemocompatible up to 30 mM. Overall, this study suggests that naringin might be an outstanding biocompatible natural component to effectively treat multidrug-resistant P. aeruginosa infections and biofilms.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic insights into glutamate-induced γ-PGA biosynthesis and process optimization in Bacillus subtilis SCP017-03 for scalable production.","authors":"Wenshan Cai, Shuai Jing, Laiying Yang, Yun Wu, Wei Li, Yao Ren, Jiao Li, Fanglan Ge","doi":"10.1007/s00449-025-03234-1","DOIUrl":"https://doi.org/10.1007/s00449-025-03234-1","url":null,"abstract":"<p><p>This study focuses on the glutamate-dependent strain Bacillus subtilis SCP017-03, systematically investigating its metabolic mechanism for synthesizing γ-polyglutamic acid (γ-PGA) in the presence of exogenous glutamate, as well as optimizing its fermentation conditions. Metabolomic analysis revealed that glutamate addition significantly altered the cellular metabolic profile, with 480 out of 1674 metabolites showing differential expression. Notably, pathways such as the TCA cycle, glycolysis, glutathione metabolism, and amino acid metabolism were significantly upregulated, enhancing precursor supply and energy metabolism, thereby promoting γ-PGA synthesis. Based on these findings, fermentation conditions were optimized in a 5-L bioreactor. Yeast extract was identified as the optimal nitrogen-rich nutrient, and at an addition level of 7.5 g/L, the γ-PGA yield reached 87 g/L. The optimal conversion efficiency and yield were achieved with a 5% addition of monosodium glutamate. Molecular weight analysis showed that the resulting γ-PGA predominantly ranged from 1071 to 4897 kDa, making it suitable for agricultural applications. In a 30-L scale-up fermentation, γ-PGA production reached 71 g/L through optimized aeration, agitation, and feeding strategies, demonstrating the scalability of the process. Finally, optimized spray-drying conditions (inlet temperature of 160 °C) resulted in a 67% recovery rate with a desirable product appearance. This study provides important metabolic regulation strategies and engineering optimization foundations for the efficient industrial production of γ-PGA.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Operational efficiencies and sustainable bioprocessing in electro-fermentation and microbial fuel cells.","authors":"Bhanu Kushwaha, Ravi Shukla, Rakesh Kumar Sharma","doi":"10.1007/s00449-025-03228-z","DOIUrl":"https://doi.org/10.1007/s00449-025-03228-z","url":null,"abstract":"<p><p>The development of innovative bioprocessing technologies has resulted from the growing global need for sustainable forms of energy and environmentally friendly waste treatment. In this review, we focus on the combined electro-fermentation and microbial fuel cells, as they form a hybrid system that simultaneously addresses wastewater treatment, bioenergy production, and bioplastics. Even though microbial fuel cells produce electricity out of the organic waste by the use of electroactive microorganisms, electro-fermentation improves the microbial pathways through the external electrochemical management. The novelty of the review is that it compares the two technologies in detail and identifies the synergistic potential of the technologies as well as assesses the efficiencies of their operations, scalability, and impact on the environment. The research utilizing Scopus and PubMed directories was done by means of a systematic literature review that included 147 peer-reviewed experimentation and technology-oriented studies published during the period of 2012-2024. The main results lead to the conclusion that integrated systems imply significant increase in power densities (up to 2000 mW/m²), the enhancement of electron transfer efficiency (increased by 30-40%), large-scale production of useful products such as methane, hydrogen and organic acids. In spite of this promise, there are still difficulties regarding microbial stability, material costs, and energy balance. The review identifies the existing gaps and future opportunities, which include the development of novel electrode materials, the employment of better reactor designs and designer microbial consortia. The combination of such systems may become an interesting strategy of the next generation of biorefineries and have a good prospect to become a part of the circular economy and climate as a whole.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel algae-assisted sequencing batch and intermittent air-lift bioreactor (A-SBIAB) using polyester filament-based carriers for piggery wastewater treatment.","authors":"Jia Liu, Cong Du, Nan Xu, Chuanqi Shi, Baoling Liu, Bingjie Tu, Keyuan Zhang, Kang Gao","doi":"10.1007/s00449-025-03231-4","DOIUrl":"10.1007/s00449-025-03231-4","url":null,"abstract":"<p><p>Algae-assisted biological wastewater treatment technology has been widely applied in wastewater treatment due to its low cost and great removal performance. However, the stabilization and sustainability of the alga-bacteria symbiosis system still need to be developed. In this work, an algae-assisted sequencing batch and intermittent air-lift bioreactor (A-SBIAB) system was constructed for removing the nutrients from the piggery wastewater. A strengthened algae-bacterial symbiosis system was also achieved with the aid of a suspended bio-carrier composed of polyester filament fixed on concentric plastic rings, which provided enhanced surface area and illumination access for microbial attachment. The removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were up to 92.0%, 81.7% and 89.3%, respectively, at the optimum parameters (Chl-a concentration of 1000 mg/m³, light intensity of 6000 lx and lighting time 10 h). The Campylobacteria (72.05%), Desulfuromonadia (11.16%), Spirochaetia (3.10%) and Bacteroidia (1.73%) as the dominant bacterial communities would be responsible for the nitrate ammonification, nitrogen fixation, nitrate reduction and organics degradation, respectively. Meanwhile, Chlorophyceae (98.21%) became the overwhelming algal community, playing a positive effect on the nutrients removal.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}