Bioprocess and Biosystems Engineering最新文献

{"title":"Fed-batch bioreactor intensifies degradation and detoxification of anthraquinone dyes by an immobilized white-rot fungal consortium.","authors":"Pragnya Paramita Sahoo, Sangeeta Raut, Aswinee Kumar Panda, Adyasha Anapurba Sahoo, Sudip Kumar Sen","doi":"10.1007/s00449-026-03439-y","DOIUrl":"https://doi.org/10.1007/s00449-026-03439-y","url":null,"abstract":"<p><p>The textile industry produces enormous quantities of highly polluted wastewater that is usually contaminated with recalcitrant dyes, salts, and toxic metals; hence, it urgently requires efficient and sustainable treatment strategies. Considering the above scenario, mycoremediation using an immobilized white rot fungal consortium (WRFC) of Trametes cubensis OR394063, Polyporus umbellatus OR398647, Leiotrametes flavida MTCC 12927, and Pleurotus pulmonarius MTCC 1805 was used in the present study to treat Anthraquinone (AQ) dye-induced synthetic textile effluent (STE) in batch, continuous, and fed-batch bioreactors. The prepared STE was composed of 200 mg/L mixed AQ dyes (Acid Blue 129, Alizarin Cyanin Green, Remazol Brilliant Blue R) along with high levels of salts, surfactants and heavy metals to simulate real textile wastewater. Among the three reactor configurations, the highest decolorization efficiency (99.06 ± 3.5%) of AQ dyes was observed in the fed-batch reactor within 120 h, followed by continuous (93.68 ± 3.5%), and batch (91.99 ± 3.6%). Enhanced activities of laccase (0.67 ± 0.01 U/mL), Lignin Peroxidase (LiP) (79.25 ± 0.33 U/mL), and Manganese Peroxidase (MnP) (157.35 ± 0.53 U/mL) were observed within 64 h in the fed-batch reactor. The kinetic studies showed that the degradation of AQ dyes in all reactors followed pseudo-first-order kinetics. Also, significant reduction in COD (75.04 ± 0.50%), BOD (96.05 ± 0.25%), TDS (65.96 ± 0.15%) and heavy metals such as Cd (50.00 ± 2.40%) and Pb (39.47 ± 2.20%) were observed in the fed-batch reactor compared to the batch and continuous systems. Moreover, the toxicity assessments using Vigna radiata and Allium cepa showed significant decrease in the toxicity of the treated effluent, particularly for the fed-batch process as evidenced by increased germination index (99.39 ± 0.29%), decrease in the phytotoxicity % (14.51 ± 0.31%), relative toxicity (16.12 ± 0.33%) and mitotic index (8.89 ± 0.19%) and reduced chromosomal aberrations (7.35 ± 0.28%).</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fundamental aspects of lignocellulosic material as slow-release electron donor for biological sulfate reduction.","authors":"Raul Natale, Rachel B Costa, Tiago P Delforno, Kelly J Dussan, Guilherme Salvador Peres Neto, Denise Bevilaqua","doi":"10.1007/s00449-026-03440-5","DOIUrl":"https://doi.org/10.1007/s00449-026-03440-5","url":null,"abstract":"<p><p>Biological sulfate reduction combined with chemical precipitation of metal sulfides is an good alternative to mitigate environmental impacts of acid mine drainage (AMD) and to recover base metals. This process requires a consistent supply of electron donors, and this study investigated the use of Brewery Spent Grain (BSG) as a slow-release electron donor (SRED) to sustain sulfate reduction and enhance metal sulfide precipitation as settling particles. A microbial consortium was enriched in batch reactors using poultry manure as the primary inoculum to evaluate its performance, metabolic pathways, and resilience to sulfide toxicity. Consortium enrichment successfully promoted a synergistic consortium where fermentative bacteria, notably Macellibacteroides and Enterococcus, hydrolyzed the lignocellulosic BSG to release lactate, hydrogen, propionate, and butyrate. These substrates were subsequently utilized by SRB groups, including Bacteroides graminisolvens, Desulforhabdus sp., and Desulfomicrobium, to drive sulfate reduction. The enrichment process increased the sulfide concentration and the sulfate removal rate. Optimization assays revealed that a ratio of 8 g BSG/g SO₄^2- provided the best conditions for sulfate removal. Conversely, excess BSG led to reactor acidification, slowing performance and causing excessive gas production. Sulfide toxicity tests demonstrated that while high sulfide accumulation may increase the lag phase, it does not significantly interfere with the ultimate sulfate consumption rate. The sum of those results provides optimized parameters for biogenic sulfide generation using BSG, giving a new perspective on the integration of industrial by-products into circular AMD remediation, establishing a foundation for more sustainable mining practices aligned with future global demands.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149163587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community succession and functional trait reorganization in a continuous aerobic denitrification reactor under repeated hydraulic retention time reductions.","authors":"Zhuzhong Yin, Zhaoyue Yang, Chenxiang Sun, Zhenghua Liu","doi":"10.1007/s00449-026-03432-5","DOIUrl":"https://doi.org/10.1007/s00449-026-03432-5","url":null,"abstract":"<p><p>Aerobic denitrification enables nitrogen removal under oxygen-rich conditions, yet its microbial response to hydraulic retention time (HRT) disturbance remains unclear. Here, a continuous aerobic denitrification reactor inoculated with activated sludge enriched in aerobic denitrifiers was operated by stepwise shortening of HRT from 108 h to 60 h. 16S rRNA gene amplicon sequencing and metagenomic sequencing were used to characterize microbial succession, functional adaptation, and co-occurrence network reorganization. Nitrogen removal efficiency remained above 80% under a high influent nitrogen load of 300 mg N/L, comprising about 150 mg/L NH₄^⁺-N and 150 mg/L NO₃^⁻-N, despite marked taxonomic turnover. The alpha diversity of the microbial community decreased from 3.01 ± 0.13 to 2.24 ± 0.38, whereas metagenomic gene diversity remained relatively stable at approximately 13.30 ± 0.12, showing that gene-level diversity did not decline in parallel with microbial diversity. Dominant aerobic denitrifiers shifted from Aquimonas to Pseudofulvimonas. Network analysis showed that Aquimonas contributed more to within-module connectivity, whereas Pseudofulvimonas exhibited stronger among-module connectivity under short HRT. Short HRT enriched genes related to biofilm formation, adhesion, fimbriae, membrane proteins, and pilus assembly. These results indicate that community succession, functional trait reorganization, and redistribution of network roles accompanied stable aerobic denitrification under hydraulic disturbance.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of basal media and feeds on CHO cell cultures: a full factorial experiment implicating tryptophan in medium-feed antagonism.","authors":"Daria Kalkova, Polina Maximova, Anna Kolokhina, Layaly Shkair, Manar Ammar, Mikhail Samsonov, Daniel Bayzigitov","doi":"10.1007/s00449-026-03442-3","DOIUrl":"https://doi.org/10.1007/s00449-026-03442-3","url":null,"abstract":"<p><p>Chinese Hamster Ovary (CHO) cells are the top choice for recombinant protein production in modern biomanufacturing. Selecting the optimal basal media and feeds during the upstream process development is vital for the target product yield and quality, an irrational screening strategy might often result in inefficiencies and development delays. Here we report a full factorial screening of basal media and feeds combinations on four monoclonal antibody expressing CHO cell lines. A statistically significant interaction of the \"medium × feed\" factors, observed in 48% of the analyzed responses (p < 0.05), confirms their non-additivity and the impracticality of single-factor approaches. We identified a critical combination demonstrating consistently low efficacy in all the analyzed antibodies. The liquid chromatography-mass spectrometry (LC-MS/MS) analysis of its composition revealed, that this combination creates a complex metabolic imbalance: a critical deficiency of tryptophan, a pronounced gradient of taurine (absence in the medium with a high content in the feed) and a similar imbalance of alanine. The findings confirm the need for full-factorial screening when choosing basal media and feeds, and also lay the foundation for a systematic design of culture media within the identified limitations.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-efficiency secretion expression of FAD-dependent glucose dehydrogenase in Pichia pastoris via signal peptide optimization and redox cofactor enhancement.","authors":"Chun Wei, Zhenao Zhang, Jiahao Xu, Jianyong Zheng, Tianhao He, Yuqi Wang, Dongchen Liu, Xueting Chen","doi":"10.1007/s00449-026-03443-2","DOIUrl":"https://doi.org/10.1007/s00449-026-03443-2","url":null,"abstract":"<p><p>FAD-dependent glucose dehydrogenase (FAD-GDH) is a promising glucose sensing enzymes owing to its excellent thermostability and substrate specificity. To produce recombinant FAD-GDH with higher enzymatic activity by Pichia pastoris to meet commercial demands, three complementary strategies were proposed. First, the OST signal peptide, which outperformed ADV and MSB, replaced α-MF to yield much more efficient secretion, enhancing enzyme activity and protein yield up to 201.4% and 214.0% compared to the levels obtained with the original secretion signal, respectively, in shake-flask cultures. Second, to address the low specific activity of FAD-GDH, mixed feeding with riboflavin, adenosine, and yeast extract during induction period of fermentation had improved enzyme activity and specific activity to196.6% and 161.4%, respectively, while maintaining cell viability, compared with the control without additional feeding. Third, to further enhance specific activity of FAD-GDH, endogenous FAD synthase was ectopically overexpressed under the control of either the GAP or AOX1 promoter via CRISPR-Cas9-mediated genomic integration. The strain with AOX1-drivenFAD synthase (WCG-AOX1-FAD1) exhibited the most significant improvements, with enzyme activity of 1023.46 ± 95.85 U/mL (239.4% of WCG-OST) and specific activity of 886.31 ± 25.42 U/mg (233.4% of WCG-OST), both representing relatively high levels among similar studies. The synergistic application of these three strategies provides a reference for the industrial production of various cofactor-dependent enzymes.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A three-step serum-free protocol for the robust generation of monocyte-derived dendritic cells from human hematopoietic stem/progenitor cells.","authors":"Tsung-Yu Tseng, Hsing-Fen Tsai, Yun Chen, Keng-Fu Hsu, Wei-Lun Huang, Chieh-Tsung Lo, Po-Ming Wu, Chao-Ling Yao","doi":"10.1007/s00449-026-03441-4","DOIUrl":"https://doi.org/10.1007/s00449-026-03441-4","url":null,"abstract":"<p><p>Dendritic cells (DCs) and their monocyte precursors are rare in peripheral blood, which limits their use in immunotherapy. Although hematopoietic stem/progenitor cells (HSPCs) provide an alternative source, they are even less abundant in vivo. To address this limitation and compensate for apoptosis during the generation of DCs from monocytes, we developed a three-step serum-free protocol (culture system) that combines HSPC expansion with sequential differentiation. The protocol comprises a 7-day HSPC expansion phase, a 14-day monocyte induction phase, and a 7-day DC generation phase-5 days of differentiation followed by 2 days of maturation with either tumor necrosis factor-α or lipopolysaccharide. Cell quantification revealed that the system generated approximately 5,000 monocytes and 50 DCs from a single HSPC. The generated DCs expressed the key surface markers CD40 and CD209 and exhibited antigen uptake capacity comparable to that of native CD14⁺ monocyte-derived DCs. Overall, this system provides a reproducible and scalable platform for studying DC biology and supports applications in preclinical and translational immunotherapy research.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of hyaluronic acid production through co-culture of mutant Streptococcus zooepidemicus and Streptococcus pyogenes under controlled uv and environmental conditions.","authors":"Hilal Girgin Öz, Hamdi Öğüt","doi":"10.1007/s00449-026-03436-1","DOIUrl":"https://doi.org/10.1007/s00449-026-03436-1","url":null,"abstract":"<p><p>This study aimed to investigate the effects of UV radiation intensity, exposure time, and distance on HA production in phenotypically selected UV-derived strains of Streptococcus zooepidemicus and Streptococcus pyogenes, and to determine the optimal environmental and co-culture conditions for maximum HA yield. Wild-type strains of S. zooepidemicus and S. pyogenes were exposed to varying UV intensities (8 W and 30 W), exposure times, and distances, followed by phenotypic screening of resulting isolates. Following sequential phenotypic screening and quantitative HA evaluation, the highest-producing UV-derived isolates were selected for subsequent fermentation optimization. Fermentations were then carried out at different temperatures and pH values to determine the optimal conditions for growth and HA synthesis. Additionally, competitive fermentations were conducted using co-cultures of phenotypically selected UV-derived S. zooepidemicus (MSz) and S. pyogenes (MSp) at varying inoculation ratios. Optimal HA production for S. zooepidemicus was observed at 37 °C and pH 7, with maximum yield achieved under 30 W UV radiation from a 30 cm distance for 12 s. S. pyogenes exhibited the highest HA production at 35 °C and pH 8 under 30 W UV applied from 25 cm for 8 s. Co-culturing MSz and MSp at a 1:1 inoculation ratio significantly enhanced HA yield compared to monocultures, suggesting a potential beneficial interaction associated with enhanced HA biosynthesis. The overall maximum HA concentration was 1421.38 mg/L, obtained from the 1:1 co-culture of MSz and MSp at 37 °C and pH 6.5, corresponding to an average volumetric productivity of 0.237 g/L/h over the 6-h fermentation period. Temperature, pH, and UV exposure parameters play a pivotal role in optimizing HA production. Moreover, the co-culture of MSz and MSp presents a promising strategy for further development of HA production processes, offering higher productivity than monoculture fermentations.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elicitation and scale-up of Bacopa saponin production by plant cell suspension culture in stirred tank bioreactor.","authors":"Jayashree Nanda, Binod B Sahu, V R Singh, Nivedita Patra","doi":"10.1007/s00449-026-03435-2","DOIUrl":"https://doi.org/10.1007/s00449-026-03435-2","url":null,"abstract":"<p><p>Cell suspension (CS) culture of Bacopa monnieri produces potential anti-Alzheimer's drug bacoside A. Various elicitors at different concentrations (salicylic acid, methyl jasmonate, Yeast extract, copper sulphate and sodium chloride) were added in cell suspension culture of B. monnieri on day 5. Salicylic acid, methyl jasmonate and copper sulphate showed the highest increase in bacoside A content which was 3-5 folds higher than the control. The effect of a combination of these three elicitors was studied on the bacoside yield using Response Surface Methodology. The cultivation of B. monnieri cell suspension culture was carried out using optimized elicitor combination in a stirred tank bioreactor. This led to 2.62-fold improvement in bacoside A yield (123.63 ± 5.14 mg/L) as compared to control batch bioreactor culture (47.25 mg/L) in 10 days of cultivation period. This study not only shows the role of elicitors for enhancement of bacoside A production but also shows the synergistic effect of elicitors on Bacopa monnieri CS culture.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streamlined stable CHO cell line development using droplet microfluidics with image-based monoclonality assessment.","authors":"Kumar Vishven Naveen, Akanksha Tyagi, Omnia Mohammed Hamid Ibrahium, Rainer E A W Fischer, Raluca Ostafe","doi":"10.1007/s00449-026-03406-7","DOIUrl":"10.1007/s00449-026-03406-7","url":null,"abstract":"<p><p>Rapid generation of monoclonal CHO cell lines remains a key bottleneck in mammalian cell line development (CLD), often requiring months of iterative screening and cloning. Here, we present an integrated droplet microfluidic workflow for high-throughput screening and isolation of antibody-producing ExpiCHO-S cells using a FRET-based secretion assay. The FRET assay was developed and optimized for picodroplet-based screening by evaluating probe configuration, donor-to-acceptor ratio, antibody concentration range, and signal saturation behavior. The platform enables picodroplet-based detection of secreted antibodies, sequential enrichment of antibody-producing cells, and image-verified single-cell isolation. Application of this approach yielded image-verified monoclonal antibody-producing clones that maintained consistent antibody production during the stability assessment performed in this study, including in the absence of selection pressure. By reducing the early clone-identification phase from DNA transfection to validated 96-well antibody-producing clones to approximately 5 weeks, this workflow addresses key limitations of conventional approaches and provides both a practical FRET assay-development framework and a scalable strategy for streamlining early CLD workflows in biomanufacturing.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2703-2719"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemico-biological cascade catalysis of fructose into 2,5-bis(hydroxymethyl)furan in aqueous solution of polyethylene glycol-1000.","authors":"Jun Xia, Yun Wang, Yan Huang, Sijia Jiang, Minyu Zhu, Chen Shen, Yuanfang Deng, Kai Sun, Zhongyang Qiu, Jiaxing Xu, Xiaoyan Liu","doi":"10.1007/s00449-026-03408-5","DOIUrl":"10.1007/s00449-026-03408-5","url":null,"abstract":"<p><p>Chemico-biological cascade catalysis of hexose into 2,5-bis(hydroxymethyl)furan (BHMF) is desirable but limited by the incompatibility between the solvent and whole-cell catalyst. In this work, aqueous polyethylene glycol (PEG-1000) solution was proven to facilitate both chemical dehydration of fructose and bioreduction of 5-hydroxymethylfurfural (HMF). A high HMF yield of 0.80 was obtained from fructose using 50 mol% HCl in 60 wt% PEG-1000, in which PEG-1000 significantly restrained the formation of by-products. Nuclear magnetic resonance (NMR) analysis revealed that PEG formed hydrogen bonds with HMF and disrupted the original water-water hydrogen bond network. Such dual regulatory effects restricted the nucleophilic attack of water molecules, thereby efficiently inhibiting HMF rehydration. On the other hand, PEG-1000 was biocompatible to E. cloacae HA188, which retained 91% relative activity when it was exposed to 15 wt% PEG-1000 solution, thus the diluted crude HMF was reduced to BHMF at 0.89 yield by E. cloacae HA188 at 35℃. Finally, 454 mM of fructose was converted to BHMF with an overall yield of 0.71 by a tandem chemobiocatalytic approach under mild conditions, providing a sustainable strategy for the production of valuable furan-based chemicals.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"2733-2745"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}