Efficient pretreatment of Phragmites australis biomass using glutamic acid for bioethanol production by a hybrid hydrolysis and fermentation strategy.

Abstract

Microbial fermentation of renewable lignocellulosic biomass to produce biofuels presents significant environmental advantages. The conversion of cellulose and hemicellulose into fermentable sugars provides essential carbon sources for microbial metabolism. However, the recalcitrance of biomass limits enzymatic accessibility. In this study, mild L-glutamic acid (GA) pretreatment was applied to Phragmites australis residues (reed straw) to fractionate lignin and polysaccharides for enhancing enzymatic hydrolysis. Pretreatment with 0.20 mol/L GA at 180 °C for 50 min (logRo = 4.1) achieved glucan recovery and xylan removal rates of 84.2% and 87.8%. Consequently, glucose and total sugar yields reached 75.5 and 71.2%, representing 5.35- and 5.18-fold increases compared to untreated reed. The 28.7 g fermentable sugars with a high glucose-to-xylose ratio (18.1 g/g) were obtained from 100 g reed. The hydrolysates were subsequently used as substrates for bioethanol production by Saccharomyces cerevisiae, which yielded 12.4-32.3 g/L ethanol via separate hydrolysis and fermentation (SHF). By analyzing bioethanol production of SHF and simultaneous saccharification and fermentation (SSF), an optimized hybrid hydrolysis and fermentation (HHF) strategy was developed. Under HHF process, 48.5 g/L of ethanol was achieved from 20 wt% solid loads. This study demonstrates an efficient approach to convert abundant lignocellulosic waste into fermentable sugars and biofuels.