Dual roles of anion exchange resin in green lignocellulose biorefinery system based on self-providing acid pretreatment.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-07-01 Epub Date: 2025-04-17 DOI:10.1007/s00449-025-03166-w

Yang Lv, Haijiong Lu, Alfred Elikem Kwami Afedzi, Ikram Ul Haq, Yong Xu

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引用次数: 0

Abstract

Resting cell-catalyzed xylonic acid (XA) offers a promising strategy for developing an efficient lignocellulose biorefinery that relies on a systematic self-providing acid pretreatment. Xylo-oligosaccharides, XA, and glucose are co-produced from hemicellulose and cellulose components through a combination of XA pretreatment corncob, resting cell catalysis of xylose, and enzymatic hydrolysis of cellulose. However, the inevitable formation of degraded inhibitors and XA can synergistically impede biological processes during XA recycling and pretreatment. An anion exchange resin serves a dual function role, acting as an intelligent regulator of inhibitors and XA in the resting cell catalysis of pretreated hydrolysates, and efficiently facilitating the centrifugal separation and recycling of bacterial cells for xylose conversion to XA. After five consecutive rounds of co-recovery of the resin and bacterial cells, 91.6% of the bacteria remained viable, and the xylose conversion to XA yield reached 82.2%.

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阴离子交换树脂在自备酸预处理绿色木质纤维素生物炼制体系中的双重作用。

静息细胞催化的木酮酸（XA）为开发高效的木质纤维素生物炼制提供了一种有前途的策略，该策略依赖于系统的自提供酸预处理。低聚木糖、XA和葡萄糖是由半纤维素和纤维素组分通过XA预处理玉米芯、木糖静息细胞催化和纤维素酶解的组合共同产生的。然而，在XA回收和预处理过程中，不可避免地形成降解抑制剂和XA可以协同阻碍生物过程。阴离子交换树脂具有双重功能，在预处理水解液静息细胞催化过程中，作为抑制剂和XA的智能调节剂，有效促进细菌细胞的离心分离和再循环，将木糖转化为XA。连续5轮树脂与细菌细胞共回收后，细菌存活率为91.6%，木糖转化为XA的产量达到82.2%。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.