Abdullah N Alotaibi, Abdullah Al-Dakhil, Hayam A Alwabsi, Ibrahim O Althobaiti, Reda M El-Shishtawy, Yaaser Q Almulaiky
{"title":"Sustainable synthesis of alginate-cobalt ferrite nanocomposites for horseradish peroxidase immobilization: enhanced stability, reusability, and catalytic efficiency.","authors":"Abdullah N Alotaibi, Abdullah Al-Dakhil, Hayam A Alwabsi, Ibrahim O Althobaiti, Reda M El-Shishtawy, Yaaser Q Almulaiky","doi":"10.1007/s00449-025-03171-z","DOIUrl":null,"url":null,"abstract":"<p><p>This study explores the green synthesis of an alginate-cobalt ferrite (Alg-CoFe<sub>2</sub>O<sub>4</sub>) composite using Brachychiton populneus extract for horseradish peroxidase (HRP) immobilization, targeting enhanced enzyme stability and reusability. The Alg-CoFe<sub>2</sub>O<sub>4</sub> composite, synthesized via co-precipitation and calcination at 600 °C, was characterized using XRD, VSM, SEM-EDX, BET, FT-IR, and zeta potential analysis, confirming its structural, magnetic, and surface properties. HRP immobilization via glutaraldehyde crosslinking achieved a high immobilization yield of 84%, attributed to the composite's high surface area and covalent binding efficiency. Kinetic analysis revealed an increased Km (30 mM) and Vmax (5.88 µmol/min) for HRP@Alg-CoFe<sub>2</sub>O<sub>4</sub>, indicating enhanced catalytic efficiency with reduced substrate affinity. The immobilized enzyme exhibited superior tolerance to organic solvents, retaining 149% activity in n-hexane. In addition, it retained 71% activity after 10 cycles and 74% after 8 weeks of storage at 4 °C, outperforming free HRP. Optimal conditions shifted from pH 6.5 to 7.0 and from 50 to 60 °C, reflecting improved environmental resilience. This study highlights HRP@Alg-CoFe<sub>2</sub>O<sub>4</sub> as a robust biocatalyst, offering promising applications in wastewater treatment and industrial bioprocesses.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-025-03171-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study explores the green synthesis of an alginate-cobalt ferrite (Alg-CoFe2O4) composite using Brachychiton populneus extract for horseradish peroxidase (HRP) immobilization, targeting enhanced enzyme stability and reusability. The Alg-CoFe2O4 composite, synthesized via co-precipitation and calcination at 600 °C, was characterized using XRD, VSM, SEM-EDX, BET, FT-IR, and zeta potential analysis, confirming its structural, magnetic, and surface properties. HRP immobilization via glutaraldehyde crosslinking achieved a high immobilization yield of 84%, attributed to the composite's high surface area and covalent binding efficiency. Kinetic analysis revealed an increased Km (30 mM) and Vmax (5.88 µmol/min) for HRP@Alg-CoFe2O4, indicating enhanced catalytic efficiency with reduced substrate affinity. The immobilized enzyme exhibited superior tolerance to organic solvents, retaining 149% activity in n-hexane. In addition, it retained 71% activity after 10 cycles and 74% after 8 weeks of storage at 4 °C, outperforming free HRP. Optimal conditions shifted from pH 6.5 to 7.0 and from 50 to 60 °C, reflecting improved environmental resilience. This study highlights HRP@Alg-CoFe2O4 as a robust biocatalyst, offering promising applications in wastewater treatment and industrial bioprocesses.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
