Bioprocess and Biosystems Engineering最新文献_第4页

Mixed sugars to a multipurpose chemical: perspectives of 2,3-butanediol fermentability in complex lignocellulose hydrolysates. 混合糖的多用途化学：2,3-丁二醇在复杂木质纤维素水解物中的发酵性的观点。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-01-03 DOI: 10.1007/s00449-025-03269-4

N Vignesh

{"title":"Mixed sugars to a multipurpose chemical: perspectives of 2,3-butanediol fermentability in complex lignocellulose hydrolysates.","authors":"N Vignesh","doi":"10.1007/s00449-025-03269-4","DOIUrl":"10.1007/s00449-025-03269-4","url":null,"abstract":"2,3-Butanediol (2,3-BDO) is recognized for its industrial competitiveness and is predominantly produced in its enantiomerically pure form via microbial fermentation. The economical production of 2,3-BDO relies on the effective use of complex lignocellulosic materials and the advancement of inhibitor-resistant microbial strains. The robust expression of 2,3-BDO dehydrogenase in bacteria enhances volumetric productivity in inhibitor-rich lignocellulose hydrolysate. For instance, isolation of an inhibitor-resistant Klebsiella pneumoniae from palm oil effluent facilitated the synthesis of 75 g L⁻¹ of 2,3-BDO through separate hydrolysis and fermentation process. Conversely, the electrochemical detoxification of sugarcane bagasse hydrolysate increased the production of 2,3-BDO to 114.3 g L⁻¹ in Enterobacter aerogenes. Furthermore, deletion of glucose transporter (ptsG) in 2,3-BDO-producing bacteria mitigated carbon catabolite repression (CCR). Adaptive evolution of Paenibacillus polymyxa in non-detoxified wheat straw hydrolysate enhanced 2,3-BDO productivity to 0.72 g L⁻¹ h⁻¹. However, the rational engineering of yeast is complex, encompassing the heterologous expressions of xylose metabolism, 2,3-BDO dehydrogenase, and the deletion of the Crabtree effect. Nevertheless, partial disruption of the Crabtree effect in polyploid Saccharomyces cerevisiae resulted in increased production of 2,3-BDO (132 g L⁻¹) during the fed-batch fermentation of cassava hydrolysate. This review paper discusses the benefits and drawbacks of 2,3-BDO metabolism in both bacteria and yeast. The paper seeks to clarify the differences in 2,3-BDO production between detoxified and non-detoxified lignocellulosic hydrolysates. Further, the study illustrates the importance of generating 2,3-BDO from untreated lignocellulose via the development of microbial consortia. Economic factors that facilitate the commercialization of 2,3-BDO fermentation have been discussed in detail.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"777-799"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofortification-driven process intensification in Qingxiangxing Baijiu fermentation: synergistic enhancement of liquor yield, flavor profile, and microbial community dynamics. 以生物强化为驱动的青香星白酒发酵过程强化：协同提高白酒产量、风味特征和微生物群落动态。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-24 DOI: 10.1007/s00449-026-03293-y

Xinrui Yin, Qiao Huang, Xiaotian Wu, Ying Han, Lei Tian, Ying Liu, Yu Li, Yicheng Mao, Xianlin Ni, Tongwei Guan

{"title":"Biofortification-driven process intensification in Qingxiangxing Baijiu fermentation: synergistic enhancement of liquor yield, flavor profile, and microbial community dynamics.","authors":"Xinrui Yin, Qiao Huang, Xiaotian Wu, Ying Han, Lei Tian, Ying Liu, Yu Li, Yicheng Mao, Xianlin Ni, Tongwei Guan","doi":"10.1007/s00449-026-03293-y","DOIUrl":"10.1007/s00449-026-03293-y","url":null,"abstract":"While bran-free fermentation contributes to enhancing the sensory quality of Sichuan Qingxiangxing Baijiu, its process efficiency often falls short of the optimum level, particularly manifested as a low liquor yield. To address this bioprocess limitation, this study implemented a targeted microbial biofortification strategy by introducing specific exogenous strains. This approach significantly improved starch utilization efficiency, increasing the liquor yield from 42 to 55%. Simultaneously, the concentrations of key flavor esters (such as ethyl acetate and ethyl lactate) were markedly elevated, achieving a synergistic optimization of production efficiency and product flavor. System-level analysis revealed that the introduction of exogenous strains served as a key driver in reshaping the succession dynamics of the microbial community. By modulating intraspecific competition and interspecific cooperation, they reconstructed the metabolic network, which was closely associated with the earlier and higher-level formation and accumulation of volatile flavor compounds. This study applies biofortification technology to bran-free Qingxiangxing Baijiu production, offering a novel bioprocess control strategy for the simultaneous enhancement of yield and flavor in traditional solid-state fermentation systems.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"891-903"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic process optimization for enhanced biosynthesis of 2-phenylethanol using Candida sorboxylosa. 利用山梨假丝酵母加强2-苯乙醇生物合成的系统工艺优化。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1007/s00449-026-03287-w

Siva Prasath Chellapan Sundaram, Ajay Gunasekaran, C Honey Chandran, N Swathy Gopal, T V Suchithra

引用次数: 0

Development of a stable biochar-bacteria hybrid system with a non-N₂O-accumulating denitrifier for efficient high-strength nitrate wastewater treatment. 具有非n2o积累反硝化剂的稳定生物炭-细菌混合系统的开发，用于高效高强度硝酸盐废水处理。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-04 DOI: 10.1007/s00449-026-03289-8

Dewei Dai, Shuyi Chu, Qiankun Wang, Jibo Xiao

{"title":"Development of a stable biochar-bacteria hybrid system with a non-N2O-accumulating denitrifier for efficient high-strength nitrate wastewater treatment.","authors":"Dewei Dai, Shuyi Chu, Qiankun Wang, Jibo Xiao","doi":"10.1007/s00449-026-03289-8","DOIUrl":"10.1007/s00449-026-03289-8","url":null,"abstract":"Biological treatment of high-strength (above 500 mg/L NO3--N) nitrate wastewater is often limited by process instability, nitrite accumulation and nitrous oxide (N2O) emissions. This study developed a synergistic biochar-bacteria hybrid system by coupling a non-N2O-accumulating denitrifier (Citrobacter freundii XY-1) with biochar (BC550) derived from spent mushroom substrate. Pyrolyzed at 550 ℃, BC550 exhibited high electron transfer capacity and served as a multifunctional carrier, facilitating biofilm formation and enabling high-rate nitrate removal. In a continuous-flow biofilter treating 1200 mg/L NO3--N, the system maintained a nitrate removal efficiency exceeding 97.5% for over 100 days at a hydraulic retention time of 15 h and C/N ratio of 10, with effluent nitrite consistently below 3 mg/L. Microbial community analysis confirmed the stable dominance of the inoculated XY-1 strain (39.7%), demonstrating successful bioaugmentation and system resilience. This work presents a stable and environmentally friendly hybrid system for high-strength nitrate removal, achieved through the rational coupling of functional biochar with a specific beneficial microorganism to ensure high treatment efficiency and mitigate N2O emission risk.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"845-858"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the efficiency of living and non-living macroalgae biomass in removing classical and emergent contaminants from complex multi-element mixtures. 比较活体和非活体大藻生物量去除复杂多元素混合物中传统污染物和新兴污染物的效率。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-25 DOI: 10.1007/s00449-026-03292-z

Jéssica Jacinto, Daniela Tavares, Nicole Ferreira, Thainara Viana, João Pinto, Nuno Lapa, Eduarda Pereira, Bruno Henriques

{"title":"Comparing the efficiency of living and non-living macroalgae biomass in removing classical and emergent contaminants from complex multi-element mixtures.","authors":"Jéssica Jacinto, Daniela Tavares, Nicole Ferreira, Thainara Viana, João Pinto, Nuno Lapa, Eduarda Pereira, Bruno Henriques","doi":"10.1007/s00449-026-03292-z","DOIUrl":"10.1007/s00449-026-03292-z","url":null,"abstract":"Although macroalgae are promising biosorbents for the removal of various contaminants, their effectiveness in complex mixtures requires comprehensive comparative evaluation under multi-contaminant conditions. The ability of living and non-living Ulva lactuca and Gracilaria gracilis to simultaneously uptake Rare Earth Elements (REEs) (Y, La, Nd, Eu, Gd, Dy) and classical contaminants (Hg, Cd, Pb, As) from equimolar mixtures was compared. Batch sorption experiments were conducted for 72 h under optimised conditions of salinity (10) and pH (7.8), in which 5 g of living biomass and an equivalent non-living biomass (0.60-0.85 g) were exposed to contaminated seawater (1 L) under constant stirring (800 rpm). The living biomass exhibited high removal rates (> 80%) for REEs, Hg, and Pb, while for As and Cd, lower removals were achieved. Non-living biomass showed significantly lower removal ability for REE (generally < 40%). These findings suggest a two-step approach, exploiting, first, non-living biomass to remove common contaminants and, after, living biomass for bioaccumulating REEs, which could later be recovered for reuse.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"877-889"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13124917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selecting Raman spectra filtering based on an exhaustive statistical approach for inline bioprocesses monitoring using Sf9 insect cells. 基于详尽统计方法的拉曼光谱滤波选择用于Sf9昆虫细胞的在线生物过程监测。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-03-26 DOI: 10.1007/s00449-026-03301-1

Milena Miyu Teruya, Júlia Públio Rabello, Luis Giovani Oliveira Guardalini, Fernanda Angela Correia Barrence, Eutimio Gustavo Fernández Núñez

引用次数: 0

Denitrification performance enhanced by optimizing the sites of external carbon addition in a full-scale anoxic tank. 通过优化全尺寸缺氧池外加碳位置，提高了脱氮性能。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-04 DOI: 10.1007/s00449-026-03286-x

Yang Zhou, Jie Zhang, QingQing Wang, Qingyuan Wang, Junhui Liu, Xiaotong Chen, Yingying Guo, Mingming Sun, Liyun Sun, Zhaoxu Peng

{"title":"Denitrification performance enhanced by optimizing the sites of external carbon addition in a full-scale anoxic tank.","authors":"Yang Zhou, Jie Zhang, QingQing Wang, Qingyuan Wang, Junhui Liu, Xiaotong Chen, Yingying Guo, Mingming Sun, Liyun Sun, Zhaoxu Peng","doi":"10.1007/s00449-026-03286-x","DOIUrl":"10.1007/s00449-026-03286-x","url":null,"abstract":"Low influent carbon-to-nitrogen (C/N) ratios often limit denitrification in municipal wastewater treatment systems. This study evaluated denitrification performance in a full-scale anaerobic-anoxic-oxic (AAO) process equipped with a 6 m-deep anoxic tank containing spherical fixed carriers. Sludge flocs and carrier-attached biofilms were sampled at depths of 1 m, 3 m and 5 m along the horizontal flow path. Denitrification kinetics were quantified using batch tests, and microbial community structures were analyzed by 16 S rRNA gene sequencing. Sludge flocs exhibited the highest denitrification rates at 1 m, whereas biofilms performed optimally at 3 m. Along the horizontal direction, sludge flocs near the influent and external carbon dosing site showed enhanced denitrification, while biofilms downstream of the propeller demonstrated improved denitrification. Elevated dissolved oxygen (DO) introduced by internal reflow reduced the effective utilization of the external carbon source. Nitrosomonas was more abundant in sludge flocs, whereas Thauera dominated denitrifying community and peaked at 3 m in biofilms. Based on the spatial distribution of denitrification kinetics and microbial communities, the conventional \"pre and top\" carbon dosing strategy was re-evaluated, and an optimized \"post and top\" dosing strategy was proposed. This strategy reduced chemical oxygen demand (COD) consumption per unit of total nitrogen (TN) removed by 16%, providing a practical approach to enhance denitrification efficiency and external carbon utilization in full-scale anoxic tanks.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"801-811"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprocess performance and COD partitioning for PHA/EPS production from activated sludge fed with lactate, ethanol and citrate. 乳酸、乙醇和柠檬酸盐投喂活性污泥生产PHA/EPS的生物工艺性能和COD分配。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-02-20 DOI: 10.1007/s00449-026-03295-w

Teng Sun, Quan Yuan, Mengjia Xiao, Xinyu Zhang, Yingxue Sun, Mingquan Huang, Jihong Wu, Xueting Li, Liang Qian

{"title":"Bioprocess performance and COD partitioning for PHA/EPS production from activated sludge fed with lactate, ethanol and citrate.","authors":"Teng Sun, Quan Yuan, Mengjia Xiao, Xinyu Zhang, Yingxue Sun, Mingquan Huang, Jihong Wu, Xueting Li, Liang Qian","doi":"10.1007/s00449-026-03295-w","DOIUrl":"10.1007/s00449-026-03295-w","url":null,"abstract":"Lactate-, ethanol- and citrate-rich side streams are increasingly available from waste fermentation and organic-acid industries, yet their effects on carbon allocation in mixed-culture activated sludge remain insufficiently compared under identical conditions. Here, we operated three parallel sequencing batch reactors fed with lactate, ethanol, or citrate and quantified treatment performance together with chemical oxygen demand (COD) partitioning to intracellular polyhydroxyalkanoates (PHA) and extracellular polymeric substances (EPS). All reactors maintained stable removals of COD, total nitrogen, and total phosphorus above 80%. However, carbon allocation differed strongly. Biomass accumulated about 20 to 30 mg/g volatile suspended solids, corresponding to COD to PHA recoveries of 60.7% for lactate, 37.9% for ethanol, and 39.6% for citrate. Lactate minimized the non-PHA COD fraction and produced polyhydroxybutyrate-valerate with the highest hydroxyvalerate share, whereas ethanol favored polyhydroxybutyrate rich PHA and diverted more carbon to EPS and respiration. The EPS matrix was protein-dominated and more humified under ethanol, moderately protein-rich under lactate, and more polysaccharide-biased with weaker humic-like signals under citrate. Community profiling supported these shifts, with lactate enriching storage-oriented guilds, ethanol enriching denitrifying and EPS-producing guilds, and citrate supporting an enhanced biological phosphorus removal like community. Together, these results show that substrate chemistry beyond conventional volatile fatty acids can be used to steer the balance between PHA storage and EPS formation while maintaining nutrient removal.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"925-940"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146256983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of protein purity in etanercept production through process optimization in recombinant CHO cell-based transient gene expression system. 重组CHO细胞瞬时基因表达系统工艺优化提高依那西普蛋白纯度。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-04-01 Epub Date: 2026-03-09 DOI: 10.1007/s00449-026-03307-9

Hyun-Seung Kim, Hoon-Min Lee, Tae-Ho Kim, So-Hui Ryu, Mi-Jung Kang, Jungmok You, Yeon-Gu Kim

{"title":"Improvement of protein purity in etanercept production through process optimization in recombinant CHO cell-based transient gene expression system.","authors":"Hyun-Seung Kim, Hoon-Min Lee, Tae-Ho Kim, So-Hui Ryu, Mi-Jung Kang, Jungmok You, Yeon-Gu Kim","doi":"10.1007/s00449-026-03307-9","DOIUrl":"10.1007/s00449-026-03307-9","url":null,"abstract":"Maintaining high protein purity is crucial for ensuring the efficacy, safety, and productivity of therapeutic glycoproteins. Given that proteins produced within transient gene expression (TGE) systems are ultimately intended for incorporation into production cell lines developed using stable gene expression (SGE) systems, it is crucial to evaluate protein purity across both systems and explore strategies for improvement. In this study, elevated levels of both high- and low-molecular-weight impurities were observed in etanercept (ETN) produced under the evaluated TGE conditions compared with the SGE system. To address these purity concerns, we investigated the effects of various process modifications, including chemical treatments, temperature downshifts, and adjustments to kit components within the TGE system. Results indicated that rapamycin treatment, a temperature downshift to 30 °C, and the omission of Enhancer material increased the monomer proportion of ETN. Combining a temperature downshift to 30 °C with omission of the Enhancer material reduced overall ETN production but increased the monomer ratio to levels comparable to those in the SGE system. Moreover, the combination of rapamycin treatment, a temperature downshift to 30 °C, and an extended culture duration significantly enhanced both total and monomer ETN production while maintaining higher purity. These improvements were similarly observed in the HEK293 cell-based TGE system, demonstrating that the optimized culture conditions possess broad applicability across mammalian TGE systems. These findings demonstrate that comprehensive optimization of culture parameters can significantly enhance protein purity in TGE-based protein production, enabling purity levels comparable to those obtained from SGE systems under the conditions evaluated in this study.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1063-1076"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensing applications of gold-functionalized carbon-based composites. 金功能化碳基复合材料的生物传感应用。

IF 3.6 3区生物学

Bioprocess and Biosystems Engineering Pub Date : 2026-03-26 DOI: 10.1007/s00449-026-03318-6

Timothy O Ajiboye, Abolaji A Mafolasire, Idris O Sanusi, Lawrence Sawunyama, Joshua Oyetade, Sheriff A Balogun, Lebea N Nthunya, Subhendu Dhibar, Agnes Pholosi

引用次数: 0