Proteomics and bioinformatics guided discovery of microalgal multifunctional peptides for novel nutraceutical applications.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-06-25 DOI:10.1007/s00449-025-03192-8

Montassar Romdhani, Jihen Dhaouafi, Barbara Deracinois, Christophe Flahaut, Naïma Nedjar, Rafik Balti

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引用次数: 0

Abstract

This study aimed to identify and characterize bioactive peptides derived from protein hydrolysates of Arthrospira platensis (APPH) and Tetraselmis chuii (TCPH) using an integrated peptidomics and bioinformatics approach. Proteins extracted from the microalgae were hydrolyzed using pepsin (EC 3.4.23.1) at various enzyme/substrate (E/S) ratios. APPH and TCPH, prepared at an E/S ratio of 1/10 (w/w), were analyzed using peptidomics through reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with tandem mass spectrometry (MS/MS). Using the UniProtKB database, a total of 265 unique peptides were identified, including 187 peptides from APPH and 78 peptides from TCPH. Subsequent in silico analysis of these peptides revealed favorable physicochemical properties, with a notable distribution of hydrophobic (APPH: 26; TCPH: 5), amphipathic (APPH: 70; TCPH: 16), and hydrophilic peptides (APPH: 59; TCPH: 17). Toxicity assessments confirmed that none of the peptides showed hemolytic or cytotoxic risks, except for one peptide identified in TCPH with potential cytotoxicity. Furthermore, bioactivity predictions demonstrated significant multifunctional properties (scores exceeding the 0.500 threshold), identifying peptides with antihypertensive (APPH: 2; TCPH: 1), anti-diabetic (APPH: 2), anti-inflammatory (APPH: 14; TCPH: 5) and antimicrobial (APPH: 7) activities. The current study thus establishes protein hydrolysates from A. platensis and T. chuii as promising sources of bioactive peptides suitable for nutraceutical applications. Our integrated analytical and computational strategy provides critical insights into peptide multifunctionality, supporting further research and development of microalgae-derived peptides.

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蛋白质组学和生物信息学指导了微藻多功能肽在新型营养保健应用中的发现。

本研究旨在利用综合肽组学和生物信息学方法鉴定和表征platarthrospira （APPH）和Tetraselmis chuii （TCPH）蛋白水解产物的生物活性肽。用胃蛋白酶（EC 3.4.23.1）以不同的酶/底物（E/S）比水解从微藻中提取的蛋白质。以E/S比为1/10 （w/w）制备的APPH和TCPH，通过反相高效液相色谱(RP-HPLC) -串联质谱（MS/MS）对其进行肽组学分析。利用UniProtKB数据库，共鉴定出265个独特肽，其中187个来自APPH， 78个来自TCPH。随后的硅分析显示这些肽具有良好的物理化学性质，具有显著的疏水性分布(APPH: 26；TCPH: 5), amphipathic (APPH: 70；TCPH: 16)，亲水性肽(APPH: 59；TCPH: 17)。毒性评估证实，除了在TCPH中发现的一种具有潜在细胞毒性的肽外，没有任何肽显示溶血或细胞毒性风险。此外，生物活性预测显示出显著的多功能特性（得分超过0.500阈值），识别出具有抗高血压作用的肽(APPH: 2；TCPH: 1)，抗糖尿病（APPH: 2），抗炎（APPH: 14）；TCPH: 5)和抗菌（APPH: 7）活性。因此，目前的研究确定了platensis和T. chuii的蛋白质水解产物是适合营养保健应用的生物活性肽的有前途的来源。我们的综合分析和计算策略提供了对多肽多功能性的关键见解，支持微藻衍生多肽的进一步研究和开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.